pycom07g27030

IQ-DOMAIN 14-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001398
Physical Location & Seq
Reverse (-)
26872220 .. 26873586
1367 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g27030.3

Sequence Viewer

Length: 1032 bp
ATGGCAGCTCATGGTGAGGCTGGTGATGCTCTTGCAATGGCGGTGGCCACGAAAGCGGCGGCTCAGGCAGCAGTTGTCATGGTGCAAGCGGCTGTCAAAGCTGTTCGTCTTACAAAGTCTAATTGCAGGCCTTCCATTTTCGTTAGAGACAATGCTGCCATTGTTATTCAGACTGCCTTCAGAGGATATCTGGCGAGAAGAGCTCTTCGGGCGCTAAAGGGGCTGGTGAAGCTTCAAGCACTGGTGAGAGGCCACAATGTTAGAAAGAGGGCAAAGATGACTCTGCAATGCATGCTGGCTCTGGTTCGAGTACAAGCTCGTGTTCTTGACCAACGAACGAAGAGGCTTTCGCACGAAGGCAGTGCAGATTCCATTTTCAGTGATCCCACTGGCTTGTGGGGATCACATTTCTCAGACAGAAGGGAGTCCATTTCGAAAAACGAAGAGAGGAGCGCCGCGGATGATTTGATCCGTTGGGACGATGTCAAACCGAAAACATTGGAGCAGATTCAAGCCATGTTGCAGAAAACAAAACAAGCTGCCTTGAAGCGTGAAAGGAGTACCAGCCTTGCTTATGCCTTCTCCAACCAGAAATGGAGAAGTAGTGGTAGCGGCGACGAAGATACTGGTGAGCATGAGGAGCTAGAGGAGAAGTTTAATTTGCTTGATGGTTGGACAAGAAGAAAGCAATGTGAGATCGATCCAGGCAGAGCTTCATGTGATCAGAGAGATCCCATCAAGACCGTTGAAATCGACACTTCTCGACCTCACTCTTATTCGAGTACCCCGAATTCCAATTCCAGAACCAAACGATCAGATCAATGCAATTATCAGAGCCATCATCTGCAGTATATTTATCAACAGCAAAGAGTTCATCCTCAGTCATATCCAGTTGCCTCTCCTCGATCACCCGTCACGCCATTTACTTCCGTGGCAAGAAATCTACAAGTGCACTCGGCTAGTCCCCGTTGCCTTCGTGGATCGATCATAGGATTAACCACCCAATGCCGGCATCACATACCCCAAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

344

Amino Acids

38.41

Weight (kDa)

9.96

Isoelectric Point (pI)

54.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 458
AciI CCGC 7 cut(s) 41, 56, 59, 89, 456, 458, 612
AclWI GGATC 6 cut(s) 377, 409, 463, 695, 725, 988
AcoI YGGCCR 1 cut(s) 45
AcsI RAATTY 1 cut(s) 790
AcuI CTGAAG 1 cut(s) 163
AfaI GTAC 3 cut(s) 312, 562, 784
AfiI CCNNNNNNNGG 1 cut(s) 1008
AgsI TTSAA 4 cut(s) 236, 512, 547, 749
AjnI CCWGG 1 cut(s) 703
AluBI AGCT 8 cut(s) 8, 101, 203, 232, 317, 539, 643, 713
AluI AGCT 8 cut(s) 8, 101, 203, 232, 317, 539, 643, 713
Alw21I GWGCWC 2 cut(s) 205, 954
Alw26I GTCTC 1 cut(s) 141
Alw44I GTGCAC 1 cut(s) 950
AlwI GGATC 6 cut(s) 377, 409, 463, 695, 725, 988
AoxI GGCC 3 cut(s) 45, 128, 250
ApaLI GTGCAC 1 cut(s) 950
ApeKI GCWGC 4 cut(s) 5, 68, 155, 539
ApoI RAATTY 1 cut(s) 790
AspLEI GCGC 2 cut(s) 214, 455
AsuHPI GGTGA 6 cut(s) 26, 35, 238, 256, 641, 900
AsuII TTCGAA 1 cut(s) 434
BaeGI GKGCMC 1 cut(s) 954
BalI TGGCCA 1 cut(s) 47
BanII GRGCYC 1 cut(s) 205
BauI CACGAG 1 cut(s) 318
Bbv12I GWGCWC 2 cut(s) 205, 954
BbvI GCAGC 4 cut(s) 17, 80, 142, 526
BccI CCATC 3 cut(s) 662, 743, 846
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BciT130I CCWGG 1 cut(s) 705
BclI TGATCA 1 cut(s) 721
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 2 cut(s) 644, 960
BfmI CTRYAG 1 cut(s) 845
BfoI RGCGCY 2 cut(s) 215, 456
BisI GCNGC 9 cut(s) 6, 57, 60, 69, 90, 156, 456, 540, 613
BlsI GCNGC 9 cut(s) 7, 58, 61, 70, 91, 157, 457, 541, 614
Bme1390I CCNGG 1 cut(s) 705
BmrFI CCNGG 1 cut(s) 705
BmsI GCATC 2 cut(s) 16, 1021
BplI GAGNNNNNCTC 2 cut(s) 187, 219
Bpu10I CCTNAGC 1 cut(s) 63
Bpu14I TTCGAA 1 cut(s) 434
Bsa29I ATCGAT 2 cut(s) 699, 983
BsaJI CCNNGG 2 cut(s) 456, 930
BsaXI ACNNNNNCTCC 2 cut(s) 589, 619
Bsc4I CCNNNNNNNGG 1 cut(s) 1008
Bse118I RCCGGY 1 cut(s) 1008
Bse1I ACTGG 4 cut(s) 246, 394, 631, 890
Bse3DI GCAATG 3 cut(s) 42, 293, 695
BseBI CCWGG 1 cut(s) 705
BseCI ATCGAT 2 cut(s) 699, 983
BseDI CCNNGG 2 cut(s) 456, 930
BseGI GGATG 2 cut(s) 466, 874
BseLI CCNNNNNNNGG 1 cut(s) 1008
BseMI GCAATG 3 cut(s) 42, 293, 695
BseMII CTCAG 3 cut(s) 77, 426, 893
BseNI ACTGG 4 cut(s) 246, 394, 631, 890
BseRI GAGGAG 4 cut(s) 463, 653, 662, 891
BseSI GKGCMC 1 cut(s) 954
BseXI GCAGC 4 cut(s) 17, 80, 142, 526
BsgI GTGCAG 1 cut(s) 384
Bsh1236I CGCG 1 cut(s) 458
BshFI GGCC 3 cut(s) 47, 130, 252
BshVI ATCGAT 2 cut(s) 699, 983
BsiHKAI GWGCWC 2 cut(s) 205, 954
BsiSI CCGG 1 cut(s) 1009
BslFI GGGAC 2 cut(s) 491, 948
BslI CCNNNNNNNGG 1 cut(s) 1008
BsmAI GTCTC 1 cut(s) 141
BsmFI GGGAC 2 cut(s) 491, 948
BsnI GGCC 3 cut(s) 47, 130, 252
Bsp119I TTCGAA 1 cut(s) 434
Bsp1286I GDGCHC 2 cut(s) 205, 954
BspACI CCGC 7 cut(s) 41, 56, 59, 89, 456, 458, 612
BspANI GGCC 3 cut(s) 47, 130, 252
BspCNI CTCAG 3 cut(s) 76, 425, 892
BspDI ATCGAT 2 cut(s) 699, 983
BspFNI CGCG 1 cut(s) 458
BspMAI CTGCAG 1 cut(s) 849
BspPI GGATC 6 cut(s) 377, 409, 463, 695, 725, 988
BspQI GCTCTTC 2 cut(s) 193, 210
BspT104I TTCGAA 1 cut(s) 434
BsrDI GCAATG 3 cut(s) 42, 293, 695
BsrFI RCCGGY 1 cut(s) 1008
BsrI ACTGG 4 cut(s) 246, 394, 631, 890
BssAI RCCGGY 1 cut(s) 1008
BssECI CCNNGG 2 cut(s) 456, 930
BssSI CACGAG 1 cut(s) 318
Bst2BI CACGAG 1 cut(s) 318
Bst2UI CCWGG 1 cut(s) 705
Bst4CI ACNGT 1 cut(s) 745
Bst6I CTCTTC 4 cut(s) 193, 210, 335, 438
BstAPI GCANNNNNTGC 1 cut(s) 292
BstBI TTCGAA 1 cut(s) 434
BstC8I GCNNGC 5 cut(s) 87, 128, 293, 297, 1010
BstDEI CTNAG 3 cut(s) 63, 412, 879
BstDSI CCRYGG 2 cut(s) 456, 930
BstF5I GGATG 2 cut(s) 466, 874
BstFNI CGCG 1 cut(s) 458
BstH2I RGCGCY 2 cut(s) 215, 456
BstHHI GCGC 2 cut(s) 214, 455
BstMAI GTCTC 1 cut(s) 141
BstNI CCWGG 1 cut(s) 705
BstNSI RCATGY 1 cut(s) 295
BstSCI CCNGG 1 cut(s) 703
BstSFI CTRYAG 1 cut(s) 845
BstSLI GKGCMC 1 cut(s) 954
BstUI CGCG 1 cut(s) 458
BstV1I GCAGC 4 cut(s) 17, 80, 142, 526
BstX2I RGATCY 1 cut(s) 730
BstYI RGATCY 1 cut(s) 730
Bsu15I ATCGAT 2 cut(s) 699, 983
BsuRI GGCC 3 cut(s) 47, 130, 252
BsuTUI ATCGAT 2 cut(s) 699, 983
BtgI CCRYGG 2 cut(s) 456, 930
BtsCI GGATG 2 cut(s) 466, 874
BtsI GCAGTG 1 cut(s) 367
BtsIMutI CAGTG 4 cut(s) 239, 367, 385, 387
Cac8I GCNNGC 5 cut(s) 87, 128, 293, 297, 1010
CfoI GCGC 2 cut(s) 214, 455
Cfr10I RCCGGY 1 cut(s) 1008
Cfr42I CCGCGG 1 cut(s) 459
ClaI ATCGAT 2 cut(s) 699, 983
Csp6I GTAC 3 cut(s) 311, 561, 783
CspCI CAANNNNNGTGG 2 cut(s) 24, 59
CviAII CATG 6 cut(s) 11, 79, 292, 517, 635, 717
CviQI GTAC 3 cut(s) 311, 561, 783
DdeI CTNAG 3 cut(s) 63, 412, 879
EaeI YGGCCR 1 cut(s) 45
Eam1104I CTCTTC 4 cut(s) 193, 210, 335, 438
EarI CTCTTC 4 cut(s) 193, 210, 335, 438
Ecl136II GAGCTC 1 cut(s) 203
Eco147I AGGCCT 1 cut(s) 130
Eco24I GRGCYC 1 cut(s) 205
Eco32I GATATC 1 cut(s) 188
Eco53kI GAGCTC 1 cut(s) 203
Eco57I CTGAAG 1 cut(s) 163
EcoICRI GAGCTC 1 cut(s) 203
EcoRI GAATTC 1 cut(s) 790
EcoRII CCWGG 1 cut(s) 703
EcoRV GATATC 1 cut(s) 188
EcoT22I ATGCAT 1 cut(s) 293
EcoT38I GRGCYC 1 cut(s) 205
FaeI CATG 6 cut(s) 14, 82, 295, 520, 638, 720
FaqI GGGAC 2 cut(s) 491, 948
FatI CATG 6 cut(s) 10, 78, 291, 516, 634, 716
FbaI TGATCA 1 cut(s) 721
Fnu4HI GCNGC 9 cut(s) 6, 57, 60, 69, 90, 156, 456, 540, 613
FokI GGATG 2 cut(s) 473, 861
FriOI GRGCYC 1 cut(s) 205
Fsp4HI GCNGC 9 cut(s) 6, 57, 60, 69, 90, 156, 456, 540, 613
FspBI CTAG 2 cut(s) 644, 960
GlaI GCGC 2 cut(s) 213, 454
GluI GCNGC 9 cut(s) 6, 57, 60, 69, 90, 156, 456, 540, 613
HaeII RGCGCY 2 cut(s) 215, 456
HaeIII GGCC 3 cut(s) 47, 130, 252
HapII CCGG 1 cut(s) 1009
HhaI GCGC 2 cut(s) 214, 455
Hin1II CATG 6 cut(s) 14, 82, 295, 520, 638, 720
Hin6I GCGC 2 cut(s) 212, 453
HinP1I GCGC 2 cut(s) 212, 453
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 4 cut(s) 280, 368, 425, 508
HpaII CCGG 1 cut(s) 1009
HphI GGTGA 6 cut(s) 26, 35, 238, 256, 641, 900
Hpy166II GTNNAC 1 cut(s) 952
Hpy188I TCNGA 6 cut(s) 171, 182, 415, 726, 817, 834
Hpy188III TCNNGA 4 cut(s) 326, 739, 762, 801
Hpy8I GTNNAC 1 cut(s) 952
Hpy99I CGWCG 1 cut(s) 620
HpyAV CCTTC 6 cut(s) 141, 187, 350, 414, 589, 983
HpyCH4III ACNGT 1 cut(s) 745
HpyF3I CTNAG 3 cut(s) 63, 412, 879
Hsp92II CATG 6 cut(s) 14, 82, 295, 520, 638, 720
HspAI GCGC 2 cut(s) 212, 453
KroI GCCGGC 1 cut(s) 1008
KroNI GCCGGC 1 cut(s) 1010
Ksp22I TGATCA 1 cut(s) 721
KspI CCGCGG 1 cut(s) 459
LguI GCTCTTC 2 cut(s) 193, 210
LmnI GCTCC 3 cut(s) 450, 502, 640
Lsp1109I GCAGC 4 cut(s) 17, 80, 142, 526
LweI GCATC 2 cut(s) 16, 1021
MaeI CTAG 2 cut(s) 644, 960
MaeIII GTNAC 1 cut(s) 913
MboII GAAGA 6 cut(s) 197, 210, 352, 455, 632, 693
MflI RGATCY 1 cut(s) 730
MhlI GDGCHC 2 cut(s) 205, 954
MlsI TGGCCA 1 cut(s) 47
MluCI AATT 5 cut(s) 121, 658, 790, 796, 826
MluNI TGGCCA 1 cut(s) 47
MlyI GAGTC 2 cut(s) 274, 434
MmeI TCCRAC 2 cut(s) 609, 653
Mox20I TGGCCA 1 cut(s) 47
Mph1103I ATGCAT 1 cut(s) 293
MroNI GCCGGC 1 cut(s) 1008
MscI TGGCCA 1 cut(s) 47
MseI TTAA 2 cut(s) 657, 995
Msp20I TGGCCA 1 cut(s) 47
MspA1I CMGCKG 1 cut(s) 458
MspI CCGG 1 cut(s) 1009
MspR9I CCNGG 1 cut(s) 705
MvaI CCWGG 1 cut(s) 705
MvnI CGCG 1 cut(s) 458
NaeI GCCGGC 1 cut(s) 1010
NgoMIV GCCGGC 1 cut(s) 1008
NlaIII CATG 6 cut(s) 14, 82, 295, 520, 638, 720
NmeAIII GCCGAG 1 cut(s) 935
NmuCI GTSAC 1 cut(s) 913
NsiI ATGCAT 1 cut(s) 293
NspI RCATGY 1 cut(s) 295
NspV TTCGAA 1 cut(s) 434
PaeI GCATGC 1 cut(s) 295
PceI AGGCCT 1 cut(s) 130
PciSI GCTCTTC 2 cut(s) 193, 210
PcsI WCGNNNNNNNCGW 1 cut(s) 785
PdiI GCCGGC 1 cut(s) 1010
PfeI GAWTC 2 cut(s) 368, 508
PflFI GACNNNGTC 1 cut(s) 482
PkrI GCNGC 9 cut(s) 7, 58, 61, 70, 91, 157, 457, 541, 614
PleI GAGTC 2 cut(s) 274, 433
PpsI GAGTC 2 cut(s) 274, 433
Psp124BI GAGCTC 1 cut(s) 205
Psp6I CCWGG 1 cut(s) 703
PspGI CCWGG 1 cut(s) 703
PstI CTGCAG 1 cut(s) 849
PsuI RGATCY 1 cut(s) 730
PsyI GACNNNGTC 1 cut(s) 482
RsaI GTAC 3 cut(s) 312, 562, 784
RsaNI GTAC 3 cut(s) 311, 561, 783
SacI GAGCTC 1 cut(s) 205
SacII CCGCGG 1 cut(s) 459
SapI GCTCTTC 2 cut(s) 193, 210
SaqAI TTAA 2 cut(s) 657, 995
SatI GCNGC 9 cut(s) 6, 57, 60, 69, 90, 156, 456, 540, 613
SchI GAGTC 2 cut(s) 274, 434
ScrFI CCNGG 1 cut(s) 705
SduI GDGCHC 2 cut(s) 205, 954
SetI ASST 9 cut(s) 10, 103, 205, 234, 319, 541, 645, 715, 769
SfaNI GCATC 2 cut(s) 16, 1021
SfcI CTRYAG 1 cut(s) 845
Sfr303I CCGCGG 1 cut(s) 459
SfuI TTCGAA 1 cut(s) 434
SgrBI CCGCGG 1 cut(s) 459
SphI GCATGC 1 cut(s) 295
Sse9I AATT 5 cut(s) 121, 658, 790, 796, 826
SseBI AGGCCT 1 cut(s) 130
SsiI CCGC 7 cut(s) 41, 56, 59, 89, 456, 458, 612
SspMI CTAG 2 cut(s) 644, 960
SstI GAGCTC 1 cut(s) 205
StuI AGGCCT 1 cut(s) 130
StyD4I CCNGG 1 cut(s) 703
TaaI ACNGT 1 cut(s) 745
TaqI TCGA 8 cut(s) 307, 434, 699, 753, 763, 779, 904, 983
TasI AATT 5 cut(s) 121, 658, 790, 796, 826
TatI WGTACW 1 cut(s) 310
TauI GCSGC 5 cut(s) 59, 62, 92, 458, 615
TfiI GAWTC 2 cut(s) 368, 508
Tru1I TTAA 2 cut(s) 657, 995
Tru9I TTAA 2 cut(s) 657, 995
TscAI CASTG 4 cut(s) 246, 367, 385, 394
TseFI GTSAC 1 cut(s) 913
TseI GCWGC 4 cut(s) 5, 68, 155, 539
Tsp45I GTSAC 1 cut(s) 913
TspDTI ATGAA 2 cut(s) 705, 863
TspGWI ACGGA 2 cut(s) 461, 919
TspRI CASTG 4 cut(s) 246, 367, 385, 394
Tth111I GACNNNGTC 1 cut(s) 482
VneI GTGCAC 1 cut(s) 950
XapI RAATTY 1 cut(s) 790
XceI RCATGY 1 cut(s) 295
XspI CTAG 2 cut(s) 644, 960
Zsp2I ATGCAT 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.