Rh7BG087500

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001398
Physical Location & Seq
Reverse (-)
6453166 .. 6455272
2107 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG087500.1

Sequence Viewer

Length: 1488 bp
ATGGGAAAGAAAAGTGGTAGTACTTCATGGTTGACTATAGTCAAAAGAGCCTTCTGGTCTCCGTCTAAGGATCATGACCAAAAGAGCAGCCGAAGAAGAGAAGACCATGAACCAGAAGAGGAGGAAAAGAAGAGAGAGAAGAGAAGATGGCTATTCCGAAAGCCTACTACACATGTTCAACAATGTGAAGCAACAAAGACAGAAACAACCATAGCTGTGACTCCTGTTTTGGCTGCTGACCAAAAACATGCAATTGCTGTGGCCGTAGCCACTGCAGCTGCGGCCGAAGCAGCTGTTGCAACTGCCCAAGCGGCAGTAGAAATTGTTCGTCTCACTAGGCCGAACAATTTTGTCAAAGAGCATTATGCTGCTACACTCATTCAAACAGCTTTCAGAGGTTACTTGGCGAGGAGAGCTCTGCGTGCGCTTAAGGGGCTTGTAAAGCTCCAAGCTTTAGTGAGAGGTCACAATGTTCGAAAGCAAGCAAAGTTGACACTGAAGTGCATGCAAGCTCTGGTTCGAGTTCAAGATCAGGTTCGGAACGAAAGAGCCAGGCTTTCGCATGAAGGAGGCAGAAACTCCATGTTTTCCGAAACAGACATGAGCCTATGGGAGTGCAGATACCTTCAAGACATTCGCGACAGAAAGTCGATTTCTAGAGAGAAAGTGAGTGAAGGAGTTGACCAATTCTTGTTGCAAAATCGAAAGGAAGCTTCTTTAAAACGTGAAAAAGCACTTGCTTATGCCTTTTCTCACCAGATATGGAGGCCTAGAAGACACCCATCTGTAGGGGACGAAGCAGAGATAGAAGAGAGAACAAAATGGCTTGATCGGTGGATGGCTACAAAAAATTGGGAAAATTACAGAGCTTCAACCGACAAAAGAGACTCCATAAAAACTGTTGAAATGGACACTTCTAGGCCTTACGTTTCATCCCAACACCAAAAACAGCCAAACCCAAATGCTGCAATGGCTTCACCTTTGCACAAATCACATTACAATAACATGAACATCTCTCCCAGCCACTCACCTGCAACACCCTCTCCTTCAAAACCCAGATCGCTAAACGTGCGTTCAGCTAGTCCAAGATGCTCTTCAGCAGCACACACACCAAACTTGGGCTCTGCTTACTGCTTCAGGGGTGGTATGTGTAGATATGGAGTGGGCTCAAATGGGGTTACTAATAATGTCATTGGTTCAGTGCCTAATTACATGGCTGCAACCGAGTCTGCAAAGGCCAAGGCTCGATCTCTAAGTGCACCACGGTCAAGGCCATCGACACCAGATAGAGACCGGGTTGGTTCGGCCAAGAAACGGCTTTCGTACCCGGCCCCTGAGTCACATAGCTTGAGAAGTCCTAGCTTCAAGAGTGTGCAAAATGGGTACTATGGAAGGGACTCAAGGGAGAACTTGTCTACTTACACTGATAGCCTAGGGGGAGAAATTTCTCCATGTTCAACCACTGACTTAAGGTGGTTACAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

495

Amino Acids

55.73

Weight (kDa)

10.1

Isoelectric Point (pI)

63.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 364 - 451 5e-18 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1039
Acc36I ACCTGC 1 cut(s) 1039
AccI GTMKAC 1 cut(s) 1415
AccII CGCG 1 cut(s) 639
AciI CCGC 2 cut(s) 281, 311
AclWI GGATC 1 cut(s) 78
AcoI YGGCCR 3 cut(s) 261, 282, 1305
AcsI RAATTY 1 cut(s) 1443
AcuI CTGAAG 3 cut(s) 518, 1080, 1120
AfaI GTAC 3 cut(s) 22, 1325, 1385
AfiI CCNNNNNNNGG 3 cut(s) 788, 1118, 1314
AflII CTTAAG 2 cut(s) 428, 1468
AflIII ACRYGT 1 cut(s) 172
AgsI TTSAA 9 cut(s) 179, 383, 527, 629, 873, 905, 1050, 1366, 1458
AhdI GACNNNNNGTC 1 cut(s) 646
AjnI CCWGG 1 cut(s) 551
AjuI GAANNNNNNNTTGG 2 cut(s) 1078, 1110
AleI CACNNNNGTG 1 cut(s) 499
Alw21I GWGCWC 2 cut(s) 418, 1261
Alw26I GTCTC 4 cut(s) 63, 335, 879, 1284
Alw44I GTGCAC 1 cut(s) 1257
AlwI GGATC 1 cut(s) 78
AoxI GGCC 9 cut(s) 261, 282, 338, 767, 920, 1236, 1271, 1305, 1329
ApaLI GTGCAC 1 cut(s) 1257
ApeKI GCWGC 9 cut(s) 87, 233, 275, 278, 290, 368, 965, 1100, 1217
ApoI RAATTY 1 cut(s) 1443
ArsI GACNNNNNNTTYG 2 cut(s) 211, 243
Asp700I GAANNNNTTC 1 cut(s) 324
AspA2I CCTAGG 1 cut(s) 1432
AspLEI GCGC 1 cut(s) 427
AspS9I GGNCC 1 cut(s) 1330
AsuC2I CCSGG 2 cut(s) 1295, 1328
AsuHPI GGTGA 3 cut(s) 746, 969, 1020
AsuII TTCGAA 1 cut(s) 475
AvrII CCTAGG 1 cut(s) 1432
BaeGI GKGCMC 1 cut(s) 1261
BanII GRGCYC 3 cut(s) 418, 1124, 1169
BbsI GAAGAC 2 cut(s) 108, 781
Bbv12I GWGCWC 2 cut(s) 418, 1261
BbvI GCAGC 9 cut(s) 99, 220, 265, 287, 302, 355, 952, 1112, 1204
BccI CCATC 4 cut(s) 141, 790, 832, 1282
BceAI ACGGC 2 cut(s) 248, 1331
BciT130I CCWGG 1 cut(s) 553
BcnI CCSGG 2 cut(s) 1295, 1328
BcoDI GTCTC 4 cut(s) 63, 335, 879, 1284
BfaI CTAG 7 cut(s) 336, 657, 771, 918, 1080, 1359, 1433
BfmI CTRYAG 3 cut(s) 36, 273, 786
BfrI CTTAAG 2 cut(s) 428, 1468
BfuAI ACCTGC 1 cut(s) 1039
BglI GCCNNNNNGGC 1 cut(s) 311
BlnI CCTAGG 1 cut(s) 1432
BmcAI AGTACT 1 cut(s) 22
Bme1390I CCNGG 3 cut(s) 553, 1295, 1328
BmeRI GACNNNNNGTC 1 cut(s) 646
BmgT120I GGNCC 1 cut(s) 1330
BmiI GGNNCC 1 cut(s) 1332
BmrFI CCNGG 3 cut(s) 553, 1295, 1328
BmsI GCATC 1 cut(s) 1079
BoxI GACNNNNGTC 1 cut(s) 38
BpiI GAAGAC 2 cut(s) 108, 781
BplI GAGNNNNNCTC 2 cut(s) 400, 432
Bpu14I TTCGAA 1 cut(s) 475
BpuEI CTTGAG 2 cut(s) 1369, 1384
BpuMI CCSGG 2 cut(s) 1295, 1328
BsaI GGTCTC 2 cut(s) 63, 1284
BsaJI CCNNGG 3 cut(s) 1239, 1262, 1432
BsaXI ACNNNNNCTCC 4 cut(s) 1027, 1057, 1397, 1427
Bsc4I CCNNNNNNNGG 3 cut(s) 788, 1118, 1314
Bse3DI GCAATG 1 cut(s) 975
BseBI CCWGG 1 cut(s) 553
BseDI CCNNGG 3 cut(s) 1239, 1262, 1432
BseGI GGATG 2 cut(s) 843, 932
BseLI CCNNNNNNNGG 3 cut(s) 788, 1118, 1314
BseMI GCAATG 1 cut(s) 975
BseMII CTCAG 1 cut(s) 1326
BseRI GAGGAG 2 cut(s) 134, 424
BseSI GKGCMC 1 cut(s) 1261
BseX3I CGGCCG 1 cut(s) 282
BseXI GCAGC 9 cut(s) 99, 220, 265, 287, 302, 355, 952, 1112, 1204
BseYI CCCAGC 1 cut(s) 1019
BsgI GTGCAG 1 cut(s) 637
Bsh1236I CGCG 1 cut(s) 639
Bsh1285I CGRYCG 1 cut(s) 285
BshFI GGCC 9 cut(s) 263, 284, 340, 769, 922, 1238, 1273, 1307, 1331
BsiEI CGRYCG 1 cut(s) 285
BsiHKAI GWGCWC 2 cut(s) 418, 1261
BsiSI CCGG 2 cut(s) 1294, 1328
BslFI GGGAC 2 cut(s) 806, 1409
BslI CCNNNNNNNGG 3 cut(s) 788, 1118, 1314
BsmAI GTCTC 4 cut(s) 63, 335, 879, 1284
BsmBI CGTCTC 1 cut(s) 335
BsmFI GGGAC 2 cut(s) 806, 1409
BsnI GGCC 9 cut(s) 263, 284, 340, 769, 922, 1238, 1273, 1307, 1331
Bso31I GGTCTC 2 cut(s) 63, 1284
Bsp119I TTCGAA 1 cut(s) 475
Bsp1286I GDGCHC 4 cut(s) 418, 1124, 1169, 1261
Bsp143I GATC 5 cut(s) 70, 529, 829, 1058, 1247
Bsp68I TCGCGA 1 cut(s) 639
BspACI CCGC 2 cut(s) 281, 311
BspANI GGCC 9 cut(s) 263, 284, 340, 769, 922, 1238, 1273, 1307, 1331
BspCNI CTCAG 1 cut(s) 1327
BspFNI CGCG 1 cut(s) 639
BspHI TCATGA 1 cut(s) 73
BspLI GGNNCC 1 cut(s) 1332
BspMAI CTGCAG 1 cut(s) 277
BspMI ACCTGC 1 cut(s) 1039
BspPI GGATC 1 cut(s) 78
BspQI GCTCTTC 1 cut(s) 1099
BspT104I TTCGAA 1 cut(s) 475
BspTI CTTAAG 2 cut(s) 428, 1468
BspTNI GGTCTC 2 cut(s) 63, 1284
BsrDI GCAATG 1 cut(s) 975
BssECI CCNNGG 3 cut(s) 1239, 1262, 1432
BssMI GATC 5 cut(s) 70, 529, 829, 1058, 1247
BssT1I CCWWGG 2 cut(s) 1239, 1432
Bst2UI CCWGG 1 cut(s) 553
Bst4CI ACNGT 2 cut(s) 901, 1266
Bst6I CTCTTC 6 cut(s) 91, 111, 125, 134, 804, 1099
BstAFI CTTAAG 2 cut(s) 428, 1468
BstAPI GCANNNNNTGC 1 cut(s) 296
BstBI TTCGAA 1 cut(s) 475
BstC8I GCNNGC 4 cut(s) 423, 483, 506, 510
BstDEI CTNAG 3 cut(s) 66, 1253, 1335
BstDSI CCRYGG 1 cut(s) 1262
BstF5I GGATG 2 cut(s) 843, 932
BstFNI CGCG 1 cut(s) 639
BstHHI GCGC 1 cut(s) 427
BstKTI GATC 5 cut(s) 73, 532, 832, 1061, 1250
BstMAI GTCTC 4 cut(s) 63, 335, 879, 1284
BstMBI GATC 5 cut(s) 70, 529, 829, 1058, 1247
BstMCI CGRYCG 1 cut(s) 285
BstNI CCWGG 1 cut(s) 553
BstNSI RCATGY 3 cut(s) 176, 251, 508
BstPAI GACNNNNGTC 1 cut(s) 38
BstSCI CCNGG 3 cut(s) 551, 1293, 1326
BstSFI CTRYAG 3 cut(s) 36, 273, 786
BstSLI GKGCMC 1 cut(s) 1261
BstUI CGCG 1 cut(s) 639
BstV1I GCAGC 9 cut(s) 99, 220, 265, 287, 302, 355, 952, 1112, 1204
BstV2I GAAGAC 2 cut(s) 108, 781
BstZI CGGCCG 1 cut(s) 282
BsuRI GGCC 9 cut(s) 263, 284, 340, 769, 922, 1238, 1273, 1307, 1331
BtgI CCRYGG 1 cut(s) 1262
BtsCI GGATG 2 cut(s) 843, 932
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 5 cut(s) 270, 494, 1206, 1422, 1461
BtuMI TCGCGA 1 cut(s) 639
BveI ACCTGC 1 cut(s) 1039
Cac8I GCNNGC 4 cut(s) 423, 483, 506, 510
CciI TCATGA 1 cut(s) 73
CfoI GCGC 1 cut(s) 427
Cfr13I GGNCC 1 cut(s) 1330
Csp6I GTAC 3 cut(s) 21, 1324, 1384
CspCI CAANNNNNGTGG 2 cut(s) 240, 275
CviQI GTAC 3 cut(s) 21, 1324, 1384
DdeI CTNAG 3 cut(s) 66, 1253, 1335
DpnI GATC 5 cut(s) 72, 531, 831, 1060, 1249
DpnII GATC 5 cut(s) 70, 529, 829, 1058, 1247
DraI TTTAAA 1 cut(s) 720
DriI GACNNNNNGTC 1 cut(s) 646
EaeI YGGCCR 3 cut(s) 261, 282, 1305
EagI CGGCCG 1 cut(s) 282
Eam1104I CTCTTC 6 cut(s) 91, 111, 125, 134, 804, 1099
Eam1105I GACNNNNNGTC 1 cut(s) 646
EarI CTCTTC 6 cut(s) 91, 111, 125, 134, 804, 1099
Ecl136II GAGCTC 1 cut(s) 416
EclXI CGGCCG 1 cut(s) 282
Eco130I CCWWGG 2 cut(s) 1239, 1432
Eco147I AGGCCT 2 cut(s) 769, 922
Eco24I GRGCYC 3 cut(s) 418, 1124, 1169
Eco31I GGTCTC 2 cut(s) 63, 1284
Eco52I CGGCCG 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 416
Eco57I CTGAAG 3 cut(s) 518, 1080, 1120
EcoICRI GAGCTC 1 cut(s) 416
EcoRII CCWGG 1 cut(s) 551
EcoT14I CCWWGG 2 cut(s) 1239, 1432
EcoT38I GRGCYC 3 cut(s) 418, 1124, 1169
ErhI CCWWGG 2 cut(s) 1239, 1432
Esp3I CGTCTC 1 cut(s) 335
FalI AAGNNNNNCTT 2 cut(s) 1078, 1110
FaqI GGGAC 2 cut(s) 806, 1409
FblI GTMKAC 1 cut(s) 1415
FokI GGATG 2 cut(s) 850, 919
FriOI GRGCYC 3 cut(s) 418, 1124, 1169
FspBI CTAG 7 cut(s) 336, 657, 771, 918, 1080, 1359, 1433
GlaI GCGC 1 cut(s) 426
GsaI CCCAGC 1 cut(s) 1023
HaeIII GGCC 9 cut(s) 263, 284, 340, 769, 922, 1238, 1273, 1307, 1331
HapII CCGG 2 cut(s) 1294, 1328
HhaI GCGC 1 cut(s) 427
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HincII GTYRAC 3 cut(s) 33, 492, 682
HindII GTYRAC 3 cut(s) 33, 492, 682
HindIII AAGCTT 2 cut(s) 450, 711
HinfI GANTC 5 cut(s) 220, 887, 1226, 1337, 1397
HpaII CCGG 2 cut(s) 1294, 1328
HphI GGTGA 3 cut(s) 746, 969, 1020
Hpy166II GTNNAC 5 cut(s) 33, 492, 682, 1259, 1416
Hpy188I TCNGA 4 cut(s) 158, 395, 540, 592
Hpy188III TCNNGA 6 cut(s) 74, 527, 629, 638, 657, 1366
Hpy8I GTNNAC 5 cut(s) 33, 492, 682, 1259, 1416
HpyAV CCTTC 6 cut(s) 61, 560, 635, 668, 1056, 1386
HpyCH4III ACNGT 2 cut(s) 901, 1266
HpyCH4IV ACGT 3 cut(s) 724, 927, 1068
HpyF3I CTNAG 3 cut(s) 66, 1253, 1335
HpySE526I ACGT 3 cut(s) 724, 927, 1068
HspAI GCGC 1 cut(s) 425
Kzo9I GATC 5 cut(s) 70, 529, 829, 1058, 1247
LguI GCTCTTC 1 cut(s) 1099
LmnI GCTCC 1 cut(s) 450
Lsp1109I GCAGC 9 cut(s) 99, 220, 265, 287, 302, 355, 952, 1112, 1204
LweI GCATC 1 cut(s) 1079
MaeI CTAG 7 cut(s) 336, 657, 771, 918, 1080, 1359, 1433
MaeII ACGT 3 cut(s) 724, 927, 1068
MaeIII GTNAC 6 cut(s) 217, 398, 464, 1177, 1338, 1476
MalI GATC 5 cut(s) 72, 531, 831, 1060, 1249
MboI GATC 5 cut(s) 70, 529, 829, 1058, 1247
MfeI CAATTG 1 cut(s) 252
MhlI GDGCHC 4 cut(s) 418, 1124, 1169, 1261
MluCI AATT 8 cut(s) 252, 321, 346, 686, 850, 859, 1207, 1443
MlyI GAGTC 5 cut(s) 214, 881, 1235, 1346, 1391
MnlI CCTC 8 cut(s) 112, 115, 389, 402, 455, 563, 759, 1051
MroXI GAANNNNTTC 1 cut(s) 324
MseI TTAA 3 cut(s) 429, 719, 1469
MslI CAYNNNNRTG 2 cut(s) 215, 499
MspA1I CMGCKG 2 cut(s) 278, 293
MspCI CTTAAG 2 cut(s) 428, 1468
MspI CCGG 2 cut(s) 1294, 1328
MspR9I CCNGG 3 cut(s) 553, 1295, 1328
MunI CAATTG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 553
MvnI CGCG 1 cut(s) 639
NciI CCSGG 2 cut(s) 1295, 1328
NdeII GATC 5 cut(s) 70, 529, 829, 1058, 1247
NlaIV GGNNCC 1 cut(s) 1332
NmuCI GTSAC 3 cut(s) 217, 464, 1338
NruI TCGCGA 1 cut(s) 639
NspI RCATGY 3 cut(s) 176, 251, 508
NspV TTCGAA 1 cut(s) 475
OliI CACNNNNGTG 1 cut(s) 499
PaeI GCATGC 1 cut(s) 508
PagI TCATGA 1 cut(s) 73
PaqCI CACCTGC 1 cut(s) 1039
PceI AGGCCT 2 cut(s) 769, 922
PciI ACATGT 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 1099
PdmI GAANNNNTTC 1 cut(s) 324
PleI GAGTC 5 cut(s) 214, 881, 1234, 1345, 1391
PpsI GAGTC 5 cut(s) 214, 881, 1234, 1345, 1391
PscI ACATGT 1 cut(s) 172
PshAI GACNNNNGTC 1 cut(s) 38
Psp124BI GAGCTC 1 cut(s) 418
Psp6I CCWGG 1 cut(s) 551
PspFI CCCAGC 1 cut(s) 1019
PspGI CCWGG 1 cut(s) 551
PspN4I GGNNCC 1 cut(s) 1332
PspPI GGNCC 1 cut(s) 1330
PsrI GAACNNNNNNTAC 2 cut(s) 309, 341
PstI CTGCAG 1 cut(s) 277
PvuII CAGCTG 2 cut(s) 278, 293
RruI TCGCGA 1 cut(s) 639
RsaI GTAC 3 cut(s) 22, 1325, 1385
RsaNI GTAC 3 cut(s) 21, 1324, 1384
RseI CAYNNNNRTG 2 cut(s) 215, 499
SacI GAGCTC 1 cut(s) 418
SapI GCTCTTC 1 cut(s) 1099
SaqAI TTAA 3 cut(s) 429, 719, 1469
Sau3AI GATC 5 cut(s) 70, 529, 829, 1058, 1247
Sau96I GGNCC 1 cut(s) 1330
ScaI AGTACT 1 cut(s) 22
SchI GAGTC 5 cut(s) 214, 881, 1235, 1346, 1391
ScrFI CCNGG 3 cut(s) 553, 1295, 1328
SduI GDGCHC 4 cut(s) 418, 1124, 1169, 1261
SfaNI GCATC 1 cut(s) 1079
SfcI CTRYAG 3 cut(s) 36, 273, 786
SfuI TTCGAA 1 cut(s) 475
SmiMI CAYNNNNRTG 2 cut(s) 215, 499
SmlI CTYRAG 4 cut(s) 428, 1348, 1399, 1468
SmoI CTYRAG 4 cut(s) 428, 1348, 1399, 1468
SphI GCATGC 1 cut(s) 508
Sse9I AATT 8 cut(s) 252, 321, 346, 686, 850, 859, 1207, 1443
SseBI AGGCCT 2 cut(s) 769, 922
SsiI CCGC 2 cut(s) 281, 311
SspMI CTAG 7 cut(s) 336, 657, 771, 918, 1080, 1359, 1433
SstI GAGCTC 1 cut(s) 418
StuI AGGCCT 2 cut(s) 769, 922
StyD4I CCNGG 3 cut(s) 551, 1293, 1326
StyI CCWWGG 2 cut(s) 1239, 1432
TaaI ACNGT 2 cut(s) 901, 1266
TaiI ACGT 3 cut(s) 727, 930, 1071
TaqI TCGA 6 cut(s) 475, 520, 650, 703, 1246, 1277
TasI AATT 8 cut(s) 252, 321, 346, 686, 850, 859, 1207, 1443
TatI WGTACW 1 cut(s) 20
TauI GCSGC 2 cut(s) 284, 314
Tru1I TTAA 3 cut(s) 429, 719, 1469
Tru9I TTAA 3 cut(s) 429, 719, 1469
TscAI CASTG 5 cut(s) 277, 501, 1206, 1429, 1468
TseFI GTSAC 3 cut(s) 217, 464, 1338
TseI GCWGC 9 cut(s) 87, 233, 275, 278, 290, 368, 965, 1100, 1217
Tsp45I GTSAC 3 cut(s) 217, 464, 1338
TspDTI ATGAA 5 cut(s) 15, 123, 579, 921, 1022
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 5 cut(s) 277, 501, 1206, 1429, 1468
Vha464I CTTAAG 2 cut(s) 428, 1468
VneI GTGCAC 1 cut(s) 1257
XapI RAATTY 1 cut(s) 1443
XbaI TCTAGA 1 cut(s) 656
XceI RCATGY 3 cut(s) 176, 251, 508
XmaJI CCTAGG 1 cut(s) 1432
XmiI GTMKAC 1 cut(s) 1415
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 7 cut(s) 336, 657, 771, 918, 1080, 1359, 1433
ZrmI AGTACT 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.