pycom14g11290

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001398
Physical Location & Seq
Reverse (-)
14260347 .. 14261808
1462 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g11290.2

Sequence Viewer

Length: 900 bp
ATGCAAGCTCTGGTTCGAGTACAAGATCAGGTTCACACCGAGAGATCAAGGCTTTCGCATGATGCTGGTCGAAACTCCATGTTTGCTGAAACAGGCCTTCACTTATGGGAATCCAGAATTCAGGATTTTAGTGACAGGAAGTCAATAAGTCGAAAGGAAGCTGCTTTAAAGCGCGAAAAGGCTCTTGCTTATGCTTTCTCTCATCAGATATGGAGACCTGGGAGGAACCTGTCTGCAGGGGGTGATGCAGAGATGGAAGAGAGAACAAGATGGCTAGAAAGATGGATGGCCACAAAGCAATTCGAAAACAGCAGCCGAGCTTCAATTGACAAAAGGGACTCCATAAAAACAGTTGAAATCGACACGAATTCCAGGCCTTCCTTATACACGTCTCCAAATGTTCGAATTTCGCATTCGCAACATCAAAAACAACCAGCCTCCTACGCCATTGCTTCAGCTTCACCCCTACACAAATCACAGAACATGGATCATCTCTCGCCCGCAACACCCTCTCCTTCAAAACCAAAAACTCTGAAAGTGCGGTCGGCGAGTCCAAGATGCCCAAAAGAGGAAAAATACTATTGCTCATCAGCTGCACACACACCAAGCCTATCAGGCTTCAATGCTTATTGCGGCTTCAAGGGTGCAATGTGCCGTTCTGGAACACCAAATTACATGGCTGCTACTGAATCCGCAAAAGCCAAGGCTCAATCTCTTAGTGCACCTAGAACGAGGCCTTCGACACCCGAGAGAGAAAGAGGCGGCGGTTCAGCCAAGAAACGCCTTTCGTACACTGTCCCGGCTGAGCCACAACCACATAATTGTGTTGGCATTGGTTATAGCAGCTTTAGCCATAACTTGAAAAGTCCTAGCTTCAAGAGTGTACAAAATAATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

300

Amino Acids

33.23

Weight (kDa)

9.96

Isoelectric Point (pI)

60.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 174
AciI CCGC 6 cut(s) 501, 541, 633, 693, 762, 765
AclWI GGATC 1 cut(s) 495
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 3 cut(s) 117, 367, 405
AcuI CTGAAG 1 cut(s) 438
AfaI GTAC 3 cut(s) 21, 791, 885
AfiI CCNNNNNNNGG 1 cut(s) 568
AflIII ACRYGT 1 cut(s) 387
AgsI TTSAA 7 cut(s) 324, 356, 519, 622, 640, 862, 877
AhdI GACNNNNNGTC 1 cut(s) 139
AjiI CACGTC 1 cut(s) 390
AjnI CCWGG 2 cut(s) 217, 371
AluBI AGCT 7 cut(s) 8, 161, 320, 458, 593, 846, 873
AluI AGCT 7 cut(s) 8, 161, 320, 458, 593, 846, 873
Alw21I GWGCWC 1 cut(s) 724
Alw26I GTCTC 2 cut(s) 208, 396
Alw44I GTGCAC 1 cut(s) 720
AlwI GGATC 1 cut(s) 495
Ama87I CYCGRG 1 cut(s) 746
AoxI GGCC 4 cut(s) 94, 288, 374, 734
ApaLI GTGCAC 1 cut(s) 720
ApeKI GCWGC 5 cut(s) 161, 312, 593, 680, 843
ApoI RAATTY 3 cut(s) 117, 367, 405
AspLEI GCGC 1 cut(s) 174
AsuC2I CCSGG 1 cut(s) 800
AsuHPI GGTGA 2 cut(s) 254, 453
AsuII TTCGAA 2 cut(s) 303, 403
AvaI CYCGRG 1 cut(s) 746
BaeGI GKGCMC 1 cut(s) 724
BalI TGGCCA 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 724
BbvI GCAGC 5 cut(s) 148, 324, 580, 667, 855
BccI CCATC 4 cut(s) 247, 264, 276, 280
BceAI ACGGC 1 cut(s) 639
BciT130I CCWGG 2 cut(s) 219, 373
BcnI CCSGG 1 cut(s) 800
BcoDI GTCTC 2 cut(s) 208, 396
BfaI CTAG 3 cut(s) 275, 726, 870
BfmI CTRYAG 1 cut(s) 234
BglI GCCNNNNNGGC 1 cut(s) 615
BisI GCNGC 7 cut(s) 162, 313, 594, 634, 681, 763, 844
BlpI GCTNAGC 1 cut(s) 804
BlsI GCNGC 7 cut(s) 163, 314, 595, 635, 682, 764, 845
Bme1390I CCNGG 3 cut(s) 219, 373, 800
BmeRI GACNNNNNGTC 1 cut(s) 139
BmeT110I CYCGRG 1 cut(s) 746
BmgBI CACGTC 1 cut(s) 390
BmiI GGNNCC 1 cut(s) 227
BmrFI CCNGG 3 cut(s) 219, 373, 800
BmsI GCATC 3 cut(s) 52, 235, 548
Bpu1102I GCTNAGC 1 cut(s) 804
Bpu14I TTCGAA 2 cut(s) 303, 403
BpuMI CCSGG 1 cut(s) 800
BsaI GGTCTC 1 cut(s) 208
BsaJI CCNNGG 2 cut(s) 218, 702
BsaXI ACNNNNNCTCC 2 cut(s) 496, 526
Bsc4I CCNNNNNNNGG 1 cut(s) 568
Bse3DI GCAATG 2 cut(s) 447, 654
BseBI CCWGG 2 cut(s) 219, 373
BseDI CCNNGG 2 cut(s) 218, 702
BseGI GGATG 1 cut(s) 291
BseLI CCNNNNNNNGG 1 cut(s) 568
BseMI GCAATG 2 cut(s) 447, 654
BseMII CTCAG 1 cut(s) 795
BseSI GKGCMC 1 cut(s) 724
BseXI GCAGC 5 cut(s) 148, 324, 580, 667, 855
BsgI GTGCAG 1 cut(s) 579
Bsh1236I CGCG 1 cut(s) 174
Bsh1285I CGRYCG 1 cut(s) 545
BshFI GGCC 4 cut(s) 96, 290, 376, 736
BsiEI CGRYCG 1 cut(s) 545
BsiHKAI GWGCWC 1 cut(s) 724
BsiHKCI CYCGRG 1 cut(s) 746
BsiSI CCGG 1 cut(s) 800
BslFI GGGAC 2 cut(s) 350, 782
BslI CCNNNNNNNGG 1 cut(s) 568
BsmAI GTCTC 2 cut(s) 208, 396
BsmBI CGTCTC 1 cut(s) 396
BsmFI GGGAC 2 cut(s) 350, 782
BsmI GAATGC 1 cut(s) 412
BsnI GGCC 4 cut(s) 96, 290, 376, 736
Bso31I GGTCTC 1 cut(s) 208
BsoBI CYCGRG 1 cut(s) 746
Bsp119I TTCGAA 2 cut(s) 303, 403
Bsp1286I GDGCHC 1 cut(s) 724
Bsp1407I TGTACA 1 cut(s) 883
Bsp143I GATC 3 cut(s) 25, 44, 487
Bsp1720I GCTNAGC 1 cut(s) 804
BspACI CCGC 6 cut(s) 501, 541, 633, 693, 762, 765
BspANI GGCC 4 cut(s) 96, 290, 376, 736
BspCNI CTCAG 1 cut(s) 796
BspFNI CGCG 1 cut(s) 174
BspLI GGNNCC 1 cut(s) 227
BspMAI CTGCAG 1 cut(s) 238
BspPI GGATC 1 cut(s) 495
BspT104I TTCGAA 2 cut(s) 303, 403
BspTNI GGTCTC 1 cut(s) 208
BsrDI GCAATG 2 cut(s) 447, 654
BsrGI TGTACA 1 cut(s) 883
BssECI CCNNGG 2 cut(s) 218, 702
BssMI GATC 3 cut(s) 25, 44, 487
BssT1I CCWWGG 1 cut(s) 702
Bst2UI CCWGG 2 cut(s) 219, 373
Bst4CI ACNGT 2 cut(s) 352, 796
Bst6I CTCTTC 1 cut(s) 252
BstAUI TGTACA 1 cut(s) 883
BstBI TTCGAA 2 cut(s) 303, 403
BstC8I GCNNGC 2 cut(s) 6, 501
BstDEI CTNAG 2 cut(s) 716, 804
BstF5I GGATG 1 cut(s) 291
BstFNI CGCG 1 cut(s) 174
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 3 cut(s) 28, 47, 490
BstMAI GTCTC 2 cut(s) 208, 396
BstMBI GATC 3 cut(s) 25, 44, 487
BstMCI CGRYCG 1 cut(s) 545
BstMWI GCNNNNNNNGC 3 cut(s) 443, 615, 849
BstNI CCWGG 2 cut(s) 219, 373
BstSCI CCNGG 3 cut(s) 217, 371, 798
BstSFI CTRYAG 1 cut(s) 234
BstSLI GKGCMC 1 cut(s) 724
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 5 cut(s) 148, 324, 580, 667, 855
BsuRI GGCC 4 cut(s) 96, 290, 376, 736
BtrI CACGTC 1 cut(s) 390
BtsCI GGATG 1 cut(s) 291
BtsIMutI CAGTG 1 cut(s) 792
Cac8I GCNNGC 2 cut(s) 6, 501
CfoI GCGC 1 cut(s) 174
Csp6I GTAC 3 cut(s) 20, 790, 884
CviAII CATG 4 cut(s) 59, 79, 484, 676
CviQI GTAC 3 cut(s) 20, 790, 884
DdeI CTNAG 2 cut(s) 716, 804
DpnI GATC 3 cut(s) 27, 46, 489
DpnII GATC 3 cut(s) 25, 44, 487
DraI TTTAAA 1 cut(s) 168
DriI GACNNNNNGTC 1 cut(s) 139
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 252
Eam1105I GACNNNNNGTC 1 cut(s) 139
EarI CTCTTC 1 cut(s) 252
Eco130I CCWWGG 1 cut(s) 702
Eco147I AGGCCT 3 cut(s) 96, 376, 736
Eco31I GGTCTC 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 438
Eco88I CYCGRG 1 cut(s) 746
EcoRI GAATTC 2 cut(s) 117, 367
EcoRII CCWGG 2 cut(s) 217, 371
EcoT14I CCWWGG 1 cut(s) 702
EcoT22I ATGCAT 1 cut(s) 898
ErhI CCWWGG 1 cut(s) 702
Esp3I CGTCTC 1 cut(s) 396
FaeI CATG 4 cut(s) 62, 82, 487, 679
FaqI GGGAC 2 cut(s) 350, 782
FatI CATG 4 cut(s) 58, 78, 483, 675
FauI CCCGC 1 cut(s) 508
Fnu4HI GCNGC 7 cut(s) 162, 313, 594, 634, 681, 763, 844
FokI GGATG 1 cut(s) 298
Fsp4HI GCNGC 7 cut(s) 162, 313, 594, 634, 681, 763, 844
FspBI CTAG 3 cut(s) 275, 726, 870
GlaI GCGC 1 cut(s) 173
GluI GCNGC 7 cut(s) 162, 313, 594, 634, 681, 763, 844
HaeIII GGCC 4 cut(s) 96, 290, 376, 736
HapII CCGG 1 cut(s) 800
HhaI GCGC 1 cut(s) 174
Hin1II CATG 4 cut(s) 62, 82, 487, 679
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HinfI GANTC 4 cut(s) 110, 338, 550, 689
HpaII CCGG 1 cut(s) 800
HphI GGTGA 2 cut(s) 254, 453
Hpy166II GTNNAC 4 cut(s) 34, 722, 792, 884
Hpy188I TCNGA 2 cut(s) 207, 534
Hpy188III TCNNGA 4 cut(s) 114, 122, 660, 877
Hpy8I GTNNAC 4 cut(s) 34, 722, 792, 884
HpyAV CCTTC 4 cut(s) 107, 387, 525, 747
HpyCH4III ACNGT 2 cut(s) 352, 796
HpyCH4IV ACGT 1 cut(s) 389
HpyCH4V TGCA 7 cut(s) 4, 236, 248, 596, 647, 722, 896
HpyF10VI GCNNNNNNNGC 3 cut(s) 443, 615, 849
HpyF3I CTNAG 2 cut(s) 716, 804
HpySE526I ACGT 1 cut(s) 389
Hsp92II CATG 4 cut(s) 62, 82, 487, 679
HspAI GCGC 1 cut(s) 172
Kzo9I GATC 3 cut(s) 25, 44, 487
Lsp1109I GCAGC 5 cut(s) 148, 324, 580, 667, 855
LweI GCATC 3 cut(s) 52, 235, 548
MaeI CTAG 3 cut(s) 275, 726, 870
MaeII ACGT 1 cut(s) 389
MaeIII GTNAC 1 cut(s) 131
MalI GATC 3 cut(s) 27, 46, 489
MboI GATC 3 cut(s) 25, 44, 487
MboII GAAGA 1 cut(s) 269
MfeI CAATTG 1 cut(s) 324
MhlI GDGCHC 1 cut(s) 724
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 7 cut(s) 117, 299, 324, 367, 405, 670, 820
MluNI TGGCCA 1 cut(s) 290
MlyI GAGTC 2 cut(s) 332, 559
MnlI CCTC 6 cut(s) 216, 448, 520, 562, 726, 752
Mox20I TGGCCA 1 cut(s) 290
Mph1103I ATGCAT 1 cut(s) 898
MscI TGGCCA 1 cut(s) 290
MseI TTAA 1 cut(s) 167
MslI CAYNNNNRTG 1 cut(s) 822
Msp20I TGGCCA 1 cut(s) 290
MspA1I CMGCKG 1 cut(s) 593
MspI CCGG 1 cut(s) 800
MspR9I CCNGG 3 cut(s) 219, 373, 800
MunI CAATTG 1 cut(s) 324
Mva1269I GAATGC 1 cut(s) 412
MvaI CCWGG 2 cut(s) 219, 373
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 3 cut(s) 443, 615, 849
NciI CCSGG 1 cut(s) 800
NdeII GATC 3 cut(s) 25, 44, 487
NlaIII CATG 4 cut(s) 62, 82, 487, 679
NlaIV GGNNCC 1 cut(s) 227
NmeAIII GCCGAG 1 cut(s) 341
NmuCI GTSAC 1 cut(s) 131
NsiI ATGCAT 1 cut(s) 898
NspV TTCGAA 2 cut(s) 303, 403
PceI AGGCCT 3 cut(s) 96, 376, 736
PcsI WCGNNNNNNNCGW 1 cut(s) 737
PctI GAATGC 1 cut(s) 412
PfeI GAWTC 2 cut(s) 110, 689
PkrI GCNGC 7 cut(s) 163, 314, 595, 635, 682, 764, 845
PleI GAGTC 2 cut(s) 332, 558
PpsI GAGTC 2 cut(s) 332, 558
Psp6I CCWGG 2 cut(s) 217, 371
PspGI CCWGG 2 cut(s) 217, 371
PspN4I GGNNCC 1 cut(s) 227
PstI CTGCAG 1 cut(s) 238
PvuII CAGCTG 1 cut(s) 593
RsaI GTAC 3 cut(s) 21, 791, 885
RsaNI GTAC 3 cut(s) 20, 790, 884
RseI CAYNNNNRTG 1 cut(s) 822
SaqAI TTAA 1 cut(s) 167
SatI GCNGC 7 cut(s) 162, 313, 594, 634, 681, 763, 844
Sau3AI GATC 3 cut(s) 25, 44, 487
SchI GAGTC 2 cut(s) 332, 559
ScrFI CCNGG 3 cut(s) 219, 373, 800
SduI GDGCHC 1 cut(s) 724
SfaNI GCATC 3 cut(s) 52, 235, 548
SfcI CTRYAG 1 cut(s) 234
SfuI TTCGAA 2 cut(s) 303, 403
SmiMI CAYNNNNRTG 1 cut(s) 822
Sse9I AATT 7 cut(s) 117, 299, 324, 367, 405, 670, 820
SseBI AGGCCT 3 cut(s) 96, 376, 736
SsiI CCGC 6 cut(s) 501, 541, 633, 693, 762, 765
SspMI CTAG 3 cut(s) 275, 726, 870
StuI AGGCCT 3 cut(s) 96, 376, 736
StyD4I CCNGG 3 cut(s) 217, 371, 798
StyI CCWWGG 1 cut(s) 702
TaaI ACNGT 2 cut(s) 352, 796
TaiI ACGT 1 cut(s) 392
TaqI TCGA 7 cut(s) 16, 70, 151, 303, 360, 403, 740
TasI AATT 7 cut(s) 117, 299, 324, 367, 405, 670, 820
TatI WGTACW 2 cut(s) 19, 883
TauI GCSGC 2 cut(s) 636, 765
TfiI GAWTC 2 cut(s) 110, 689
Tru1I TTAA 1 cut(s) 167
Tru9I TTAA 1 cut(s) 167
TscAI CASTG 1 cut(s) 799
TseFI GTSAC 1 cut(s) 131
TseI GCWGC 5 cut(s) 161, 312, 593, 680, 843
Tsp45I GTSAC 1 cut(s) 131
TspRI CASTG 1 cut(s) 799
VneI GTGCAC 1 cut(s) 720
XapI RAATTY 3 cut(s) 117, 367, 405
XspI CTAG 3 cut(s) 275, 726, 870
Zsp2I ATGCAT 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.