MD01G1227500.v1.1

IQ-DOMAIN 14-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001398
Physical Location & Seq
Reverse (-)
31723323 .. 31726153
2831 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1227500.v1.1.491

Sequence Viewer

Length: 1635 bp
ATGGGGAAGACTGGAGGGAGCTCCTGGTTGACCGCGGTCAAAAGGGTTTTCAGGTCTCCTACCAAGAGTGACAGAAGCAACACTAGGAGGAGAGAAGAACTGCAGGAACAAGAGGAAGAAGAAAAGAAGAGAGGAAAACGGAGATGGATTTTTAGGAAGCCTACGATCCAAGAAACGTTTATACAAGTACAACATAGAAACTGTCCAAGATCTGCTGCTGATGGCGGAGCAGCCACTCAAAATGCAACTATGGCAGCTCATGATGAGGCCCGTGATGCTCCTAGAATGGCGGTGGCCCCGACAGCAGCTGCCACAGTGCAAATGGCGGTCAAAGCTGTTCGTCTTACTAAGTCTAACTGCAGGCCTTCCATTTTCGTTAGAGACTATGCTGCCATTGTTATTCAGACTGCTTTCAGAGGATGTCTGGCTCGAAGAGCTCTTCGGGCCCTGAAGGGGCTGGTGAAGCTTCAAGCACTGGTGAGAGGCCACAATGTTAGAAAGAGGGCAAAGATGACTCTGCAATGCATGCAGGCTCTGGTTCGAGTGCAAGCTCGGGTTCTTGACCAACGAACGAAGAGGCTTTCGCATGAAGGCAGCGCAGATTCCATTTTCAGTGATCCCAATGGCTTATGTGGAGCACATTTCTCAGATAGAAGGAAGTCCATATCGAAAGACGAAGAGAGCAGCGCCTTGGATGATCTGATCCATTGGGACAACTTGAAACCGAAAACATTGGAGCAGATTGAAGCCATGTTACAGAAAACCAAGCAAGCTGCCTTGAAGAGTGAAAGGAGTACTAGCCTTGCTTATGCCTTCTCTAGCCAGAAATTGAGTAGTGGTAGCAGCGACGAAGATGCTAGTGAGCATGAGGAGTTAGAGGAGAAATTTAATTTGCTTGATGGTTGGACAAGAAGAAGGCAACGTGAGATCAATCCAGGCAGAGCTTCGTTTGATCAGAGAGATCCTATCAAAACAGTTGAAATCGACACTTCTCGACCTTACTCTTATTCGAGTACTCCGAATTCCAATTCCAGAATCAAAAGATCAGATCAATATAATTATCAGAGCCACCATCTTCAGTACAATTATCAACAGCAAAGAGCTCCTCCTCAGTCATATCCAGTTGCCTCTCCTCAATCACCCGTCACGCCATTTACTTCCATGGCAAGAAATCTACAAGTGCACTCGGCTAGTCCTCGTTGCCTTCGGGGAGATCAGAGGATTAACCACCCAATGCCACCTCCTCATACTCCCAACCAGAACTTAGGCTCAACTAATTACAACCATGGATTGATGGGGGTGCCAAACTACATGGCTACTACAGCATCAGCCAAGGCTCGGATTCGGTCTCAAAGCACACCAAGGCAAAGGCATTCAGCAAGCGAGATGGAAAAGCCAGGATCCGCAAGGAAACGCTTGTTTTTTCCAGCTCCTCATCCATGTGGTGATAGCTGTGCTAATGCTAATGTTAATGGTGGTTCGGATTATAACTTGGAGAGCCCTAGATATAACAACGGCATCAAGGGAGGCCTTGCAGTTCGAATGCCGCAGAGGCCTAGCGTGTCTTCTTGTTACACAGATGGTGAAACCTCTCCAACTTCCAGCAGCGAGCACAGAAGGTACTGGTTGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

545

Amino Acids

61.12

Weight (kDa)

10.08

Isoelectric Point (pI)

66.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 398 - 488 5.9e-16 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1488
AccB1I GGYRCC 1 cut(s) 1300
AccII CGCG 1 cut(s) 35
AciI CCGC 7 cut(s) 33, 35, 225, 290, 326, 1404, 1547
AclI AACGTT 1 cut(s) 176
AclWI GGATC 6 cut(s) 160, 611, 697, 956, 1395, 1408
AcsI RAATTY 2 cut(s) 884, 1021
AcuI CTGAAG 2 cut(s) 470, 1061
AfaI GTAC 5 cut(s) 189, 796, 1015, 1082, 1622
AfiI CCNNNNNNNGG 2 cut(s) 453, 1338
AgsI TTSAA 5 cut(s) 470, 721, 746, 781, 980
AjnI CCWGG 3 cut(s) 23, 934, 1396
Alw21I GWGCWC 6 cut(s) 23, 439, 640, 1105, 1185, 1614
Alw26I GTCTC 3 cut(s) 60, 375, 1353
Alw44I GTGCAC 1 cut(s) 1181
AlwI GGATC 6 cut(s) 160, 611, 697, 956, 1395, 1408
AlwNI CAGNNNCTG 2 cut(s) 308, 535
Ama87I CYCGRG 1 cut(s) 552
AoxI GGCC 7 cut(s) 267, 294, 362, 444, 484, 1528, 1553
ApaI GGGCCC 1 cut(s) 448
ApaLI GTGCAC 1 cut(s) 1181
ApoI RAATTY 2 cut(s) 884, 1021
AspLEI GCGC 2 cut(s) 599, 689
AspS9I GGNCC 4 cut(s) 268, 295, 444, 445
AsuHPI GGTGA 5 cut(s) 472, 490, 1131, 1457, 1595
AsuII TTCGAA 1 cut(s) 1540
AvaI CYCGRG 1 cut(s) 552
BaeGI GKGCMC 2 cut(s) 448, 1185
BamHI GGATCC 1 cut(s) 1400
BanI GGYRCC 1 cut(s) 1300
BanII GRGCYC 5 cut(s) 23, 439, 448, 1105, 1502
BarI GAAGNNNNNNTAC 2 cut(s) 738, 770
BbsI GAAGAC 2 cut(s) 14, 1557
Bbv12I GWGCWC 6 cut(s) 23, 439, 640, 1105, 1185, 1614
BccI CCATC 7 cut(s) 138, 215, 893, 1080, 1288, 1381, 1574
BceAI ACGGC 1 cut(s) 1531
BcgI CGANNNNNNTGC 2 cut(s) 836, 870
BciT130I CCWGG 3 cut(s) 25, 936, 1398
BclI TGATCA 1 cut(s) 952
BcoDI GTCTC 3 cut(s) 60, 375, 1353
BfaI CTAG 8 cut(s) 84, 282, 798, 819, 858, 1191, 1503, 1557
BfmI CTRYAG 3 cut(s) 101, 358, 1320
BfoI RGCGCY 1 cut(s) 690
BglI GCCNNNNNGGC 1 cut(s) 1552
BglII AGATCT 1 cut(s) 209
BmcAI AGTACT 2 cut(s) 796, 1015
Bme1390I CCNGG 3 cut(s) 25, 936, 1398
BmeT110I CYCGRG 1 cut(s) 552
BmgT120I GGNCC 4 cut(s) 268, 295, 444, 445
BmiI GGNNCC 4 cut(s) 297, 446, 1302, 1402
BmrFI CCNGG 3 cut(s) 25, 936, 1398
BmsI GCATC 4 cut(s) 265, 844, 1334, 1527
BoxI GACNNNNGTC 1 cut(s) 35
BpiI GAAGAC 2 cut(s) 14, 1557
BpmI CTGGAG 1 cut(s) 33
Bpu14I TTCGAA 1 cut(s) 1540
BsaI GGTCTC 2 cut(s) 60, 1353
BsaJI CCNNGG 6 cut(s) 33, 690, 1161, 1285, 1332, 1361
Bsc4I CCNNNNNNNGG 2 cut(s) 453, 1338
Bse1I ACTGG 4 cut(s) 16, 480, 1121, 1628
Bse3DI GCAATG 1 cut(s) 527
BseBI CCWGG 3 cut(s) 25, 936, 1398
BseDI CCNNGG 6 cut(s) 33, 690, 1161, 1285, 1332, 1361
BseGI GGATG 3 cut(s) 425, 700, 1435
BseLI CCNNNNNNNGG 2 cut(s) 453, 1338
BseMI GCAATG 1 cut(s) 527
BseMII CTCAG 2 cut(s) 660, 1124
BseNI ACTGG 4 cut(s) 16, 480, 1121, 1628
BseRI GAGGAG 8 cut(s) 103, 884, 893, 1095, 1098, 1122, 1233, 1422
BseSI GKGCMC 2 cut(s) 448, 1185
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 7 cut(s) 269, 296, 364, 446, 486, 1530, 1555
BshNI GGYRCC 1 cut(s) 1300
BsiHKAI GWGCWC 6 cut(s) 23, 439, 640, 1105, 1185, 1614
BsiHKCI CYCGRG 1 cut(s) 552
BslFI GGGAC 1 cut(s) 725
BslI CCNNNNNNNGG 2 cut(s) 453, 1338
BsmAI GTCTC 3 cut(s) 60, 375, 1353
BsmFI GGGAC 1 cut(s) 725
BsmI GAATGC 2 cut(s) 1372, 1548
BsnI GGCC 7 cut(s) 269, 296, 364, 446, 486, 1530, 1555
Bso31I GGTCTC 2 cut(s) 60, 1353
BsoBI CYCGRG 1 cut(s) 552
Bsp119I TTCGAA 1 cut(s) 1540
Bsp120I GGGCCC 1 cut(s) 444
Bsp1286I GDGCHC 8 cut(s) 23, 439, 448, 640, 1105, 1185, 1502, 1614
Bsp19I CCATGG 2 cut(s) 1161, 1285
BspACI CCGC 7 cut(s) 33, 35, 225, 290, 326, 1404, 1547
BspANI GGCC 7 cut(s) 269, 296, 364, 446, 486, 1530, 1555
BspCNI CTCAG 2 cut(s) 659, 1123
BspFNI CGCG 1 cut(s) 35
BspHI TCATGA 1 cut(s) 259
BspLI GGNNCC 4 cut(s) 297, 446, 1302, 1402
BspMAI CTGCAG 2 cut(s) 105, 362
BspPI GGATC 6 cut(s) 160, 611, 697, 956, 1395, 1408
BspQI GCTCTTC 2 cut(s) 427, 444
BspT104I TTCGAA 1 cut(s) 1540
BspT107I GGYRCC 1 cut(s) 1300
BspTNI GGTCTC 2 cut(s) 60, 1353
BsrDI GCAATG 1 cut(s) 527
BsrI ACTGG 4 cut(s) 16, 480, 1121, 1628
BssECI CCNNGG 6 cut(s) 33, 690, 1161, 1285, 1332, 1361
BssT1I CCWWGG 5 cut(s) 690, 1161, 1285, 1332, 1361
Bst2UI CCWGG 3 cut(s) 25, 936, 1398
Bst4CI ACNGT 3 cut(s) 203, 316, 976
Bst6I CTCTTC 6 cut(s) 122, 427, 444, 569, 672, 776
BstAPI GCANNNNNTGC 1 cut(s) 526
BstBI TTCGAA 1 cut(s) 1540
BstC8I GCNNGC 7 cut(s) 362, 527, 531, 549, 771, 1381, 1610
BstDEI CTNAG 4 cut(s) 348, 646, 1110, 1264
BstDSI CCRYGG 3 cut(s) 33, 1161, 1285
BstF5I GGATG 3 cut(s) 425, 700, 1435
BstFNI CGCG 1 cut(s) 35
BstH2I RGCGCY 1 cut(s) 690
BstHHI GCGC 2 cut(s) 599, 689
BstMAI GTCTC 3 cut(s) 60, 375, 1353
BstNI CCWGG 3 cut(s) 25, 936, 1398
BstNSI RCATGY 1 cut(s) 529
BstPAI GACNNNNGTC 1 cut(s) 35
BstSCI CCNGG 3 cut(s) 23, 934, 1396
BstSFI CTRYAG 3 cut(s) 101, 358, 1320
BstSLI GKGCMC 2 cut(s) 448, 1185
BstUI CGCG 1 cut(s) 35
BstV2I GAAGAC 2 cut(s) 14, 1557
BstX2I RGATCY 3 cut(s) 209, 961, 1400
BstYI RGATCY 3 cut(s) 209, 961, 1400
BsuRI GGCC 7 cut(s) 269, 296, 364, 446, 486, 1530, 1555
BtgI CCRYGG 3 cut(s) 33, 1161, 1285
BtsCI GGATG 3 cut(s) 425, 700, 1435
BtsIMutI CAGTG 3 cut(s) 321, 473, 619
Cac8I GCNNGC 7 cut(s) 362, 527, 531, 549, 771, 1381, 1610
CaiI CAGNNNCTG 2 cut(s) 308, 535
CciI TCATGA 1 cut(s) 259
CfoI GCGC 2 cut(s) 599, 689
Cfr13I GGNCC 4 cut(s) 268, 295, 444, 445
Cfr42I CCGCGG 1 cut(s) 36
Csp6I GTAC 5 cut(s) 188, 795, 1014, 1081, 1621
CviAII CATG 9 cut(s) 260, 526, 587, 751, 866, 1162, 1286, 1312, 1440
CviQI GTAC 5 cut(s) 188, 795, 1014, 1081, 1621
DdeI CTNAG 4 cut(s) 348, 646, 1110, 1264
Eam1104I CTCTTC 6 cut(s) 122, 427, 444, 569, 672, 776
EarI CTCTTC 6 cut(s) 122, 427, 444, 569, 672, 776
EciI GGCGGA 1 cut(s) 240
Ecl136II GAGCTC 3 cut(s) 21, 437, 1103
Eco130I CCWWGG 5 cut(s) 690, 1161, 1285, 1332, 1361
Eco147I AGGCCT 3 cut(s) 364, 1530, 1555
Eco24I GRGCYC 5 cut(s) 23, 439, 448, 1105, 1502
Eco31I GGTCTC 2 cut(s) 60, 1353
Eco53kI GAGCTC 3 cut(s) 21, 437, 1103
Eco57I CTGAAG 2 cut(s) 470, 1061
Eco88I CYCGRG 1 cut(s) 552
EcoICRI GAGCTC 3 cut(s) 21, 437, 1103
EcoO109I RGGNCCY 1 cut(s) 445
EcoRI GAATTC 1 cut(s) 1021
EcoRII CCWGG 3 cut(s) 23, 934, 1396
EcoT14I CCWWGG 5 cut(s) 690, 1161, 1285, 1332, 1361
EcoT22I ATGCAT 1 cut(s) 527
EcoT38I GRGCYC 5 cut(s) 23, 439, 448, 1105, 1502
ErhI CCWWGG 5 cut(s) 690, 1161, 1285, 1332, 1361
FaeI CATG 9 cut(s) 263, 529, 590, 754, 869, 1165, 1289, 1315, 1443
FaqI GGGAC 1 cut(s) 725
FatI CATG 9 cut(s) 259, 525, 586, 750, 865, 1161, 1285, 1311, 1439
FbaI TGATCA 1 cut(s) 952
FokI GGATG 3 cut(s) 432, 707, 1422
FriOI GRGCYC 5 cut(s) 23, 439, 448, 1105, 1502
FspBI CTAG 8 cut(s) 84, 282, 798, 819, 858, 1191, 1503, 1557
GlaI GCGC 2 cut(s) 598, 688
GsuI CTGGAG 1 cut(s) 33
HaeII RGCGCY 1 cut(s) 690
HaeIII GGCC 7 cut(s) 269, 296, 364, 446, 486, 1530, 1555
HhaI GCGC 2 cut(s) 599, 689
Hin1II CATG 9 cut(s) 263, 529, 590, 754, 869, 1165, 1289, 1315, 1443
Hin6I GCGC 2 cut(s) 597, 687
HinP1I GCGC 2 cut(s) 597, 687
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 4 cut(s) 514, 602, 1035, 1342
HphI GGTGA 5 cut(s) 472, 490, 1131, 1457, 1595
Hpy166II GTNNAC 2 cut(s) 30, 1183
Hpy188III TCNNGA 4 cut(s) 260, 560, 993, 1032
Hpy8I GTNNAC 2 cut(s) 30, 1183
Hpy99I CGWCG 1 cut(s) 851
HpyAV CCTTC 8 cut(s) 375, 445, 584, 648, 823, 909, 1214, 1611
HpyCH4III ACNGT 3 cut(s) 203, 316, 976
HpyCH4IV ACGT 2 cut(s) 176, 922
HpyF3I CTNAG 4 cut(s) 348, 646, 1110, 1264
HpySE526I ACGT 2 cut(s) 176, 922
Hsp92II CATG 9 cut(s) 263, 529, 590, 754, 869, 1165, 1289, 1315, 1443
HspAI GCGC 2 cut(s) 597, 687
Ksp22I TGATCA 1 cut(s) 952
KspI CCGCGG 1 cut(s) 36
LguI GCTCTTC 2 cut(s) 427, 444
LmnI GCTCC 8 cut(s) 18, 26, 227, 283, 635, 736, 1108, 1435
LweI GCATC 4 cut(s) 265, 844, 1334, 1527
MaeI CTAG 8 cut(s) 84, 282, 798, 819, 858, 1191, 1503, 1557
MaeII ACGT 2 cut(s) 176, 922
MaeIII GTNAC 4 cut(s) 68, 753, 1144, 1571
MflI RGATCY 3 cut(s) 209, 961, 1400
MhlI GDGCHC 8 cut(s) 23, 439, 448, 640, 1105, 1185, 1502, 1614
MluCI AATT 8 cut(s) 827, 884, 889, 1021, 1027, 1057, 1084, 1276
MlyI GAGTC 1 cut(s) 508
MmeI TCCRAC 2 cut(s) 884, 1619
Mph1103I ATGCAT 1 cut(s) 527
MseI TTAA 3 cut(s) 888, 1224, 1470
MslI CAYNNNNRTG 1 cut(s) 1440
MspA1I CMGCKG 2 cut(s) 35, 308
MspR9I CCNGG 3 cut(s) 25, 936, 1398
Mva1269I GAATGC 2 cut(s) 1372, 1548
MvaI CCWGG 3 cut(s) 25, 936, 1398
MvnI CGCG 1 cut(s) 35
NcoI CCATGG 2 cut(s) 1161, 1285
NlaIII CATG 9 cut(s) 263, 529, 590, 754, 869, 1165, 1289, 1315, 1443
NlaIV GGNNCC 4 cut(s) 297, 446, 1302, 1402
NmeAIII GCCGAG 1 cut(s) 1166
NmuCI GTSAC 2 cut(s) 68, 1144
NsiI ATGCAT 1 cut(s) 527
NspI RCATGY 1 cut(s) 529
NspV TTCGAA 1 cut(s) 1540
PaeI GCATGC 1 cut(s) 529
PagI TCATGA 1 cut(s) 259
PceI AGGCCT 3 cut(s) 364, 1530, 1555
PciSI GCTCTTC 2 cut(s) 427, 444
PcsI WCGNNNNNNNCGW 1 cut(s) 1016
PctI GAATGC 2 cut(s) 1372, 1548
PfeI GAWTC 3 cut(s) 602, 1035, 1342
PleI GAGTC 1 cut(s) 508
PpsI GAGTC 1 cut(s) 508
PshAI GACNNNNGTC 1 cut(s) 35
PsiI TTATAA 1 cut(s) 1488
Psp124BI GAGCTC 3 cut(s) 23, 439, 1105
Psp1406I AACGTT 1 cut(s) 176
Psp6I CCWGG 3 cut(s) 23, 934, 1396
PspGI CCWGG 3 cut(s) 23, 934, 1396
PspN4I GGNNCC 4 cut(s) 297, 446, 1302, 1402
PspOMI GGGCCC 1 cut(s) 444
PspPI GGNCC 4 cut(s) 268, 295, 444, 445
PstI CTGCAG 2 cut(s) 105, 362
PstNI CAGNNNCTG 2 cut(s) 308, 535
PsuI RGATCY 3 cut(s) 209, 961, 1400
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 5 cut(s) 189, 796, 1015, 1082, 1622
RsaNI GTAC 5 cut(s) 188, 795, 1014, 1081, 1621
RseI CAYNNNNRTG 1 cut(s) 1440
SacI GAGCTC 3 cut(s) 23, 439, 1105
SacII CCGCGG 1 cut(s) 36
SapI GCTCTTC 2 cut(s) 427, 444
SaqAI TTAA 3 cut(s) 888, 1224, 1470
Sau96I GGNCC 4 cut(s) 268, 295, 444, 445
ScaI AGTACT 2 cut(s) 796, 1015
SchI GAGTC 1 cut(s) 508
ScrFI CCNGG 3 cut(s) 25, 936, 1398
SduI GDGCHC 8 cut(s) 23, 439, 448, 640, 1105, 1185, 1502, 1614
SfaNI GCATC 4 cut(s) 265, 844, 1334, 1527
SfcI CTRYAG 3 cut(s) 101, 358, 1320
Sfr303I CCGCGG 1 cut(s) 36
SfuI TTCGAA 1 cut(s) 1540
SgrBI CCGCGG 1 cut(s) 36
SmiMI CAYNNNNRTG 1 cut(s) 1440
SphI GCATGC 1 cut(s) 529
Sse9I AATT 8 cut(s) 827, 884, 889, 1021, 1027, 1057, 1084, 1276
SseBI AGGCCT 3 cut(s) 364, 1530, 1555
SsiI CCGC 7 cut(s) 33, 35, 225, 290, 326, 1404, 1547
SspMI CTAG 8 cut(s) 84, 282, 798, 819, 858, 1191, 1503, 1557
SstI GAGCTC 3 cut(s) 23, 439, 1105
StuI AGGCCT 3 cut(s) 364, 1530, 1555
StyD4I CCNGG 3 cut(s) 23, 934, 1396
StyI CCWWGG 5 cut(s) 690, 1161, 1285, 1332, 1361
TaaI ACNGT 3 cut(s) 203, 316, 976
TaiI ACGT 2 cut(s) 179, 925
TaqI TCGA 7 cut(s) 430, 541, 668, 984, 994, 1010, 1540
TaqII GACCGA 1 cut(s) 1335
TasI AATT 8 cut(s) 827, 884, 889, 1021, 1027, 1057, 1084, 1276
TatI WGTACW 4 cut(s) 187, 794, 1013, 1080
TauI GCSGC 1 cut(s) 1549
TfiI GAWTC 3 cut(s) 602, 1035, 1342
Tru1I TTAA 3 cut(s) 888, 1224, 1470
Tru9I TTAA 3 cut(s) 888, 1224, 1470
TscAI CASTG 3 cut(s) 321, 480, 619
TseFI GTSAC 2 cut(s) 68, 1144
Tsp45I GTSAC 2 cut(s) 68, 1144
TspDTI ATGAA 1 cut(s) 603
TspGWI ACGGA 1 cut(s) 154
TspRI CASTG 3 cut(s) 321, 480, 619
VneI GTGCAC 1 cut(s) 1181
XapI RAATTY 2 cut(s) 884, 1021
XceI RCATGY 1 cut(s) 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 319
XspI CTAG 8 cut(s) 84, 282, 798, 819, 858, 1191, 1503, 1557
ZrmI AGTACT 2 cut(s) 796, 1015
Zsp2I ATGCAT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.