pycom01g11180

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001398
Physical Location & Seq
Reverse (-)
12071235 .. 12073467
2233 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g11180.3

Sequence Viewer

Length: 1506 bp
ATGCAGCTGGGTTTTAGTTTTACTGTATTATCTGATGGGGAGGAAGGGCGGCACTTCCTGGTTGACTGCTGTCAAAAGGGCTTTCAGATCGATCCCACTAAAGACGACGAAGATCAGAAGAAAAGAGAGAAACGAAGGTGGATTTTCAGAAAACCCACAAGTTTAAACCAACAAGAAACAGTGACCCAAGAAGCAGCCGCGGAGCAAAGGCATGTTTCAGCAGCTGCAGATCAAAAGGACGTGCTTTCTGTGGCGGTGGCCACGGCGGAGGCAGCGATGGCCACCGCCCACGCAGCCGCGGAAGTGGTTCGGCTGACTAGGCCATCCAATCACGCCAGAGAAAATTACTACGCCATTGTTATCCAGACAGCTTTCAGAGGATACTTGGCAAGGAGGGCGCTTCGTGCGCTTAAAGGGCTTGTGAAGTTGCAGGCACTGGTGAGAGGCCATAATGTCAGGAAACAAGCAAAGATGACGCTCAAGTGCATGCAGGCTCTGGTTCGAGTACAGTCTCGGGTGCTCGATCAGCGAACCAGGCTTTCTCATGAAGGCAGCAGAAAGTCTGCGTTTAGTGACACCACCAGTAGGGTTTGGGAATCGCAGTATTTTCAAGAAATTTCGGATAGGAAGTCCCTGCAGTCTAGAGAGGGAAGTAGCATAGCAGATGATTGGGACGAACGTCCTCACACCATCGAGGAAGTAAAAGCCATGTTGCAGCACAGGAAAGAAGCTGCCATGAAGCGAGAGAAATCGTTGTCCCAAGCCTTTTCTCAACAGATATGGAGGAACGGCAAGACCCCATCTATGGGAAATGAGGATGAGCTGGAAGAGAGACCGAAATGGCTGGATAGATGGATGGCCCCAAAGCCGTGGGAGAGCAGAGGAAGAGCTTCAACTGACCAGAGAGACCACATTAAAACAGTCGAAATCGACACTTCTCAGCCTTATTCAAGTTTAGCACCAAACTACAGAAGATCAAGCGCAAGCCAATACCACCAAAACCAACAACAAAGACCCAGTTCACCAAAGCAAAGACCCGGCTCACCTAAACAAAGACCCAGCTCGCCACTCCATAGAGATAGAGCCTACCAAAGCCAACCCTTTCATCATCACTCCCCTATCACACCCTCCCCATCGAAAACCCGGCCTGTCCAAGTCCGGTCTGCCAGCCCTCGTTGCATTAGAGAAGATAGAAGTTCCAATGCATCTCAAACTCCAAGCCTTAGGTCCAACTATTTCTACACTGGTGCTGTGCACCAACAACATAGCAGCAGGGCTGGTAGCACAAGCAATAGTAGCATTGCCAATTCTGGTGTTGCATTGCCTAATTACATGGCTGCAACTGAGTCTGCCAAGGCTCGGATACGCTCTCAGAGTGCACCCCGGCAAAGGCCATCTACACCAGACCGGGAACGAGCAGCCGGATCGAGCAAAGAAAAGGCTCTCGTTTCCAGCTCGATCCTTATGGTGTGGCAATTGGATATGGAGGTTATGGACACAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

502

Amino Acids

56.74

Weight (kDa)

10.06

Isoelectric Point (pI)

63.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Tree File
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 200, 299
AciI CCGC 8 cut(s) 49, 198, 200, 254, 266, 285, 297, 299
AclWI GGATC 3 cut(s) 86, 1432, 1453
AcoI YGGCCR 2 cut(s) 258, 279
AcsI RAATTY 1 cut(s) 615
AfaI GTAC 1 cut(s) 507
AfiI CCNNNNNNNGG 5 cut(s) 585, 805, 806, 1359, 1389
AgsI TTSAA 3 cut(s) 611, 894, 951
AjiI CACGTC 1 cut(s) 241
AjnI CCWGG 2 cut(s) 57, 533
AluBI AGCT 9 cut(s) 7, 224, 371, 731, 823, 890, 1062, 1455, 1502
AluI AGCT 9 cut(s) 7, 224, 371, 731, 823, 890, 1062, 1455, 1502
Alw21I GWGCWC 3 cut(s) 522, 1257, 1381
Alw26I GTCTC 3 cut(s) 516, 826, 900
Alw44I GTGCAC 2 cut(s) 1253, 1377
AlwI GGATC 3 cut(s) 86, 1432, 1453
AlwNI CAGNNNCTG 3 cut(s) 224, 436, 496
Ama87I CYCGRG 1 cut(s) 513
AoxI GGCC 7 cut(s) 258, 279, 320, 445, 858, 1145, 1391
ApaLI GTGCAC 2 cut(s) 1253, 1377
ApoI RAATTY 1 cut(s) 615
Asp700I GAANNNNTTC 2 cut(s) 306, 889
AspLEI GCGC 3 cut(s) 400, 409, 983
AspS9I GGNCC 2 cut(s) 859, 1227
AsuC2I CCSGG 4 cut(s) 1038, 1144, 1384, 1409
AsuHPI GGTGA 3 cut(s) 451, 1014, 1035
AvaI CYCGRG 1 cut(s) 513
AvaII GGWCC 1 cut(s) 1227
AxyI CCTNAGG 1 cut(s) 1223
BaeGI GKGCMC 2 cut(s) 1257, 1381
BalI TGGCCA 2 cut(s) 260, 281
Bbv12I GWGCWC 3 cut(s) 522, 1257, 1381
BccI CCATC 9 cut(s) 29, 271, 331, 698, 808, 846, 850, 1141, 1402
BceAI ACGGC 3 cut(s) 279, 805, 853
BcgI CGANNNNNNTGC 2 cut(s) 1407, 1441
BciT130I CCWGG 2 cut(s) 59, 535
BciVI GTATCC 2 cut(s) 374, 1356
BcnI CCSGG 4 cut(s) 1038, 1144, 1384, 1409
BcoDI GTCTC 3 cut(s) 516, 826, 900
BfaI CTAG 2 cut(s) 318, 642
BfmI CTRYAG 3 cut(s) 225, 635, 967
BfoI RGCGCY 1 cut(s) 401
BfuI GTATCC 2 cut(s) 374, 1356
Bme1390I CCNGG 6 cut(s) 59, 535, 1038, 1144, 1384, 1409
Bme18I GGWCC 1 cut(s) 1227
BmeT110I CYCGRG 1 cut(s) 513
BmgBI CACGTC 1 cut(s) 241
BmgT120I GGNCC 2 cut(s) 859, 1227
BmiI GGNNCC 1 cut(s) 861
BmrFI CCNGG 6 cut(s) 59, 535, 1038, 1144, 1384, 1409
BmrI ACTGGG 1 cut(s) 1011
BmsI GCATC 1 cut(s) 1214
BmuI ACTGGG 1 cut(s) 1011
BoxI GACNNNNGTC 2 cut(s) 69, 678
BpuEI CTTGAG 1 cut(s) 464
BpuMI CCSGG 4 cut(s) 1038, 1144, 1384, 1409
Bsa29I ATCGAT 1 cut(s) 90
BsaI GGTCTC 2 cut(s) 826, 900
BsaJI CCNNGG 6 cut(s) 198, 261, 297, 869, 1353, 1382
BsaWI WCCGGW 1 cut(s) 1158
Bsc4I CCNNNNNNNGG 5 cut(s) 585, 805, 806, 1359, 1389
Bse1I ACTGG 4 cut(s) 441, 582, 1017, 1249
Bse21I CCTNAGG 1 cut(s) 1223
Bse3DI GCAATG 2 cut(s) 1299, 1319
BseBI CCWGG 2 cut(s) 59, 535
BseCI ATCGAT 1 cut(s) 90
BseDI CCNNGG 6 cut(s) 198, 261, 297, 869, 1353, 1382
BseGI GGATG 3 cut(s) 323, 823, 861
BseLI CCNNNNNNNGG 5 cut(s) 585, 805, 806, 1359, 1389
BseMI GCAATG 2 cut(s) 1299, 1319
BseMII CTCAG 3 cut(s) 953, 1335, 1385
BseNI ACTGG 4 cut(s) 441, 582, 1017, 1249
BseSI GKGCMC 2 cut(s) 1257, 1381
BseYI CCCAGC 2 cut(s) 7, 1058
Bsh1236I CGCG 2 cut(s) 200, 299
BshFI GGCC 7 cut(s) 260, 281, 322, 447, 860, 1147, 1393
BshVI ATCGAT 1 cut(s) 90
BsiHKAI GWGCWC 3 cut(s) 522, 1257, 1381
BsiHKCI CYCGRG 1 cut(s) 513
BsiSI CCGG 6 cut(s) 1038, 1144, 1159, 1384, 1408, 1422
BslFI GGGAC 3 cut(s) 616, 686, 742
BslI CCNNNNNNNGG 5 cut(s) 585, 805, 806, 1359, 1389
BsmAI GTCTC 3 cut(s) 516, 826, 900
BsmFI GGGAC 3 cut(s) 616, 686, 742
BsnI GGCC 7 cut(s) 260, 281, 322, 447, 860, 1147, 1393
Bso31I GGTCTC 2 cut(s) 826, 900
BsoBI CYCGRG 1 cut(s) 513
Bsp1286I GDGCHC 3 cut(s) 522, 1257, 1381
Bsp143I GATC 8 cut(s) 87, 91, 112, 229, 523, 974, 1424, 1458
BspACI CCGC 8 cut(s) 49, 198, 200, 254, 266, 285, 297, 299
BspANI GGCC 7 cut(s) 260, 281, 322, 447, 860, 1147, 1393
BspCNI CTCAG 3 cut(s) 952, 1336, 1384
BspDI ATCGAT 1 cut(s) 90
BspFNI CGCG 2 cut(s) 200, 299
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 1 cut(s) 861
BspMAI CTGCAG 2 cut(s) 229, 639
BspPI GGATC 3 cut(s) 86, 1432, 1453
BspQI GCTCTTC 1 cut(s) 880
BspTNI GGTCTC 2 cut(s) 826, 900
BsrDI GCAATG 2 cut(s) 1299, 1319
BsrI ACTGG 4 cut(s) 441, 582, 1017, 1249
BssECI CCNNGG 6 cut(s) 198, 261, 297, 869, 1353, 1382
BssMI GATC 8 cut(s) 87, 91, 112, 229, 523, 974, 1424, 1458
BssT1I CCWWGG 1 cut(s) 1353
Bst2UI CCWGG 2 cut(s) 59, 535
Bst4CI ACNGT 4 cut(s) 25, 181, 510, 922
Bst6I CTCTTC 2 cut(s) 822, 880
BstC8I GCNNGC 6 cut(s) 432, 488, 492, 985, 1064, 1168
BstDEI CTNAG 4 cut(s) 939, 1223, 1344, 1371
BstDSI CCRYGG 4 cut(s) 198, 261, 297, 869
BstF5I GGATG 3 cut(s) 323, 823, 861
BstFNI CGCG 2 cut(s) 200, 299
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 3 cut(s) 400, 409, 983
BstKTI GATC 8 cut(s) 90, 94, 115, 232, 526, 977, 1427, 1461
BstMAI GTCTC 3 cut(s) 516, 826, 900
BstMBI GATC 8 cut(s) 87, 91, 112, 229, 523, 974, 1424, 1458
BstNI CCWGG 2 cut(s) 59, 535
BstNSI RCATGY 2 cut(s) 215, 490
BstPAI GACNNNNGTC 2 cut(s) 69, 678
BstSCI CCNGG 6 cut(s) 57, 533, 1036, 1142, 1382, 1407
BstSFI CTRYAG 3 cut(s) 225, 635, 967
BstSLI GKGCMC 2 cut(s) 1257, 1381
BstUI CGCG 2 cut(s) 200, 299
BstXI CCANNNNNNTGG 1 cut(s) 870
Bsu15I ATCGAT 1 cut(s) 90
Bsu36I CCTNAGG 1 cut(s) 1223
BsuI GTATCC 2 cut(s) 374, 1356
BsuRI GGCC 7 cut(s) 260, 281, 322, 447, 860, 1147, 1393
BsuTUI ATCGAT 1 cut(s) 90
BtgI CCRYGG 4 cut(s) 198, 261, 297, 869
BtgZI GCGATG 1 cut(s) 290
BtrI CACGTC 1 cut(s) 241
BtsCI GGATG 3 cut(s) 323, 823, 861
BtsIMutI CAGTG 3 cut(s) 186, 434, 1242
Cac8I GCNNGC 6 cut(s) 432, 488, 492, 985, 1064, 1168
CaiI CAGNNNCTG 3 cut(s) 224, 436, 496
CciI TCATGA 1 cut(s) 544
CfoI GCGC 3 cut(s) 400, 409, 983
Cfr13I GGNCC 2 cut(s) 859, 1227
Cfr42I CCGCGG 2 cut(s) 201, 300
ClaI ATCGAT 1 cut(s) 90
CseI GACGC 1 cut(s) 484
Csp6I GTAC 1 cut(s) 506
CviAII CATG 6 cut(s) 212, 487, 545, 709, 736, 1333
CviQI GTAC 1 cut(s) 506
DdeI CTNAG 4 cut(s) 939, 1223, 1344, 1371
DpnI GATC 8 cut(s) 89, 93, 114, 231, 525, 976, 1426, 1460
DpnII GATC 8 cut(s) 87, 91, 112, 229, 523, 974, 1424, 1458
DraI TTTAAA 1 cut(s) 165
EaeI YGGCCR 2 cut(s) 258, 279
Eam1104I CTCTTC 2 cut(s) 822, 880
EarI CTCTTC 2 cut(s) 822, 880
EciI GGCGGA 1 cut(s) 281
Eco130I CCWWGG 1 cut(s) 1353
Eco31I GGTCTC 2 cut(s) 826, 900
Eco47I GGWCC 1 cut(s) 1227
Eco81I CCTNAGG 1 cut(s) 1223
Eco88I CYCGRG 1 cut(s) 513
EcoRII CCWGG 2 cut(s) 57, 533
EcoT14I CCWWGG 1 cut(s) 1353
EcoT22I ATGCAT 1 cut(s) 1207
ErhI CCWWGG 1 cut(s) 1353
FaeI CATG 6 cut(s) 215, 490, 548, 712, 739, 1336
FaqI GGGAC 3 cut(s) 616, 686, 742
FatI CATG 6 cut(s) 211, 486, 544, 708, 735, 1332
FokI GGATG 3 cut(s) 310, 830, 868
FspBI CTAG 2 cut(s) 318, 642
GlaI GCGC 3 cut(s) 399, 408, 982
GsaI CCCAGC 2 cut(s) 11, 1062
HaeII RGCGCY 1 cut(s) 401
HaeIII GGCC 7 cut(s) 260, 281, 322, 447, 860, 1147, 1393
HapII CCGG 6 cut(s) 1038, 1144, 1159, 1384, 1408, 1422
HgaI GACGC 1 cut(s) 484
HhaI GCGC 3 cut(s) 400, 409, 983
Hin1II CATG 6 cut(s) 215, 490, 548, 712, 739, 1336
Hin6I GCGC 3 cut(s) 398, 407, 981
HinP1I GCGC 3 cut(s) 398, 407, 981
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HinfI GANTC 2 cut(s) 596, 1346
HpaII CCGG 6 cut(s) 1038, 1144, 1159, 1384, 1408, 1422
HphI GGTGA 3 cut(s) 451, 1014, 1035
Hpy166II GTNNAC 4 cut(s) 64, 1022, 1255, 1379
Hpy188I TCNGA 8 cut(s) 34, 87, 117, 149, 377, 622, 1362, 1374
Hpy188III TCNNGA 5 cut(s) 364, 457, 545, 611, 642
Hpy8I GTNNAC 4 cut(s) 64, 1022, 1255, 1379
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 3 cut(s) 38, 129, 542
HpyCH4III ACNGT 4 cut(s) 25, 181, 510, 922
HpyCH4IV ACGT 2 cut(s) 240, 679
HpyF3I CTNAG 4 cut(s) 939, 1223, 1344, 1371
HpySE526I ACGT 2 cut(s) 240, 679
Hsp92II CATG 6 cut(s) 215, 490, 548, 712, 739, 1336
HspAI GCGC 3 cut(s) 398, 407, 981
KspI CCGCGG 2 cut(s) 201, 300
Kzo9I GATC 8 cut(s) 87, 91, 112, 229, 523, 974, 1424, 1458
LguI GCTCTTC 1 cut(s) 880
LmnI GCTCC 1 cut(s) 202
LweI GCATC 1 cut(s) 1214
MaeI CTAG 2 cut(s) 318, 642
MaeII ACGT 2 cut(s) 240, 679
MaeIII GTNAC 2 cut(s) 181, 572
MalI GATC 8 cut(s) 89, 93, 114, 231, 525, 976, 1426, 1460
MboI GATC 8 cut(s) 87, 91, 112, 229, 523, 974, 1424, 1458
MboII GAAGA 6 cut(s) 122, 130, 839, 897, 984, 1199
MfeI CAATTG 1 cut(s) 1475
MhlI GDGCHC 3 cut(s) 522, 1257, 1381
MlsI TGGCCA 2 cut(s) 260, 281
MluCI AATT 5 cut(s) 343, 615, 1306, 1327, 1475
MluNI TGGCCA 2 cut(s) 260, 281
MlyI GAGTC 1 cut(s) 1355
MmeI TCCRAC 1 cut(s) 1254
Mox20I TGGCCA 2 cut(s) 260, 281
Mph1103I ATGCAT 1 cut(s) 1207
MroXI GAANNNNTTC 2 cut(s) 306, 889
MscI TGGCCA 2 cut(s) 260, 281
MseI TTAA 3 cut(s) 164, 411, 915
Msp20I TGGCCA 2 cut(s) 260, 281
MspA1I CMGCKG 4 cut(s) 7, 200, 224, 299
MspI CCGG 6 cut(s) 1038, 1144, 1159, 1384, 1408, 1422
MspR9I CCNGG 6 cut(s) 59, 535, 1038, 1144, 1384, 1409
MssI GTTTAAAC 1 cut(s) 165
MunI CAATTG 1 cut(s) 1475
MvaI CCWGG 2 cut(s) 59, 535
MvnI CGCG 2 cut(s) 200, 299
NciI CCSGG 4 cut(s) 1038, 1144, 1384, 1409
NdeII GATC 8 cut(s) 87, 91, 112, 229, 523, 974, 1424, 1458
NlaIII CATG 6 cut(s) 215, 490, 548, 712, 739, 1336
NlaIV GGNNCC 1 cut(s) 861
NmuCI GTSAC 2 cut(s) 181, 572
NsiI ATGCAT 1 cut(s) 1207
NspI RCATGY 2 cut(s) 215, 490
PaeI GCATGC 1 cut(s) 490
PagI TCATGA 1 cut(s) 544
PciSI GCTCTTC 1 cut(s) 880
PdmI GAANNNNTTC 2 cut(s) 306, 889
PfeI GAWTC 1 cut(s) 596
PleI GAGTC 1 cut(s) 1354
PmeI GTTTAAAC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 1354
PshAI GACNNNNGTC 2 cut(s) 69, 678
Psp6I CCWGG 2 cut(s) 57, 533
PspFI CCCAGC 2 cut(s) 7, 1058
PspGI CCWGG 2 cut(s) 57, 533
PspN4I GGNNCC 1 cut(s) 861
PspPI GGNCC 2 cut(s) 859, 1227
PstI CTGCAG 2 cut(s) 229, 639
PstNI CAGNNNCTG 3 cut(s) 224, 436, 496
PvuII CAGCTG 2 cut(s) 7, 224
RsaI GTAC 1 cut(s) 507
RsaNI GTAC 1 cut(s) 506
SacII CCGCGG 2 cut(s) 201, 300
SapI GCTCTTC 1 cut(s) 880
SaqAI TTAA 3 cut(s) 164, 411, 915
Sau3AI GATC 8 cut(s) 87, 91, 112, 229, 523, 974, 1424, 1458
Sau96I GGNCC 2 cut(s) 859, 1227
SchI GAGTC 1 cut(s) 1355
ScrFI CCNGG 6 cut(s) 59, 535, 1038, 1144, 1384, 1409
SduI GDGCHC 3 cut(s) 522, 1257, 1381
SfaNI GCATC 1 cut(s) 1214
SfcI CTRYAG 3 cut(s) 225, 635, 967
Sfr303I CCGCGG 2 cut(s) 201, 300
SgrBI CCGCGG 2 cut(s) 201, 300
SinI GGWCC 1 cut(s) 1227
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
SphI GCATGC 1 cut(s) 490
Sse9I AATT 5 cut(s) 343, 615, 1306, 1327, 1475
SsiI CCGC 8 cut(s) 49, 198, 200, 254, 266, 285, 297, 299
SspMI CTAG 2 cut(s) 318, 642
StyD4I CCNGG 6 cut(s) 57, 533, 1036, 1142, 1382, 1407
StyI CCWWGG 1 cut(s) 1353
TaaI ACNGT 4 cut(s) 25, 181, 510, 922
TaiI ACGT 2 cut(s) 243, 682
TaqI TCGA 9 cut(s) 90, 502, 522, 693, 924, 930, 1136, 1427, 1457
TaqII GACCGA 1 cut(s) 850
TasI AATT 5 cut(s) 343, 615, 1306, 1327, 1475
TatI WGTACW 1 cut(s) 505
TauI GCSGC 3 cut(s) 52, 200, 299
TfiI GAWTC 1 cut(s) 596
Tru1I TTAA 3 cut(s) 164, 411, 915
Tru9I TTAA 3 cut(s) 164, 411, 915
TscAI CASTG 3 cut(s) 186, 441, 1249
TseFI GTSAC 2 cut(s) 181, 572
Tsp45I GTSAC 2 cut(s) 181, 572
TspDTI ATGAA 3 cut(s) 561, 752, 1094
TspRI CASTG 3 cut(s) 186, 441, 1249
VneI GTGCAC 2 cut(s) 1253, 1377
VpaK11BI GGWCC 1 cut(s) 1227
XapI RAATTY 1 cut(s) 615
XbaI TCTAGA 1 cut(s) 641
XceI RCATGY 2 cut(s) 215, 490
XmnI GAANNNNTTC 2 cut(s) 306, 889
XspI CTAG 2 cut(s) 318, 642
Zsp2I ATGCAT 1 cut(s) 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.