MD06G1121100.v1.1

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001398
Physical Location & Seq
Reverse (-)
26146785 .. 26149198
2414 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1121100.v1.1.491

Sequence Viewer

Length: 1449 bp
ATGAACATGAACCAAAAGAGGAAGAAAAGAGAAAAGAGAAGGTGGCTTTTCCGGAAGCCTACTCTGCATGTTCAACAATGTGAAGCAAGGACAGTGACTGTAGATGCAAGCGCGCCTGTGAATCCTGTTTTGGATGCTGAGCAAAGGCATGCAATTGCTGTGGCCGCAGCAACTGCAGCAGTGGCTACTGCCCAAGCGGCAGTAGATATTGTTCGGCTCACAAGACCGAATAACTTTGCTAGAGAACACTATGCTGCTACACTCATTCAAACGGCATTCAGAGGTTACTTGGCAAGGAGAGCTCTACATGCACTCAAGGGGCTGGTAAAGCTTCAAGCTTTAGTGAGAGGTCATAACGTTAGGAAGCAAGCAAAGCTGACACTGAAATGCATGCAAGCTCTGGTTCGAGTACAAGATCAGGTTCGCAACGAAAGATCAAGGCTTTCGCATGACGCCGGTCGAAACTCCATGTTTGCTGAAACAGGCCTTCACTTGTGGGAATCCAGATATCTTCAGGACATTCGTGACAGAAAGTCAATATGTAGGGAGAAAAGTCGTGGTGAGGACTATTGGGACAAAGGGCTTGAACCCGTATTGCATAGTAGAAAGGAAGCTGCTTTAAAACGTGAAAAGGCTCTGGCTTATGCTTTCTCTCATCAGATATGGAGGCCTGAGAGGAACCCATCTTCAGGGGATGATGCAGAGCTAGAAGAGAGAACAAGATGGCTAGAAAGATGGATGGCCACGAAGCAATTTGAGAACAGAAGCCGAGCTTCAATTGACAAAAGGAACTCCATAAAAACAGTTGAGATCGATACGAATTCAAGGCCTTCCTCGTGCACATCTTCAAATGTCCCAATTTCATCGTCACATTCGCAACATCAAAAGCAGCTAGCCTCCTACGCCATTGCTTCAGCTTCACCCCTGCACAAATCACAGTACATGAATCATCTCTCGCCTGCAACACCCTCTCCTTCAAAACCAAAAGCTCTGAAAGTGCGGACGGCGAGTCCAAGATGCCCGAAAGAGGAAAAATACTATTGTTCATCAGCTGCACACACACCAAGCTTATCAGGCACCAATGCTTATTGGGGCTTCAGGGGTGCAATGTGCCGTTCTGGAACACCAAATTACATGGCTGCTACTGAATCCGCAAAGGCCAAGGCTCGATCGCTTAGCACACCAAAAACAAGGCCTTCTACACCCGAGAGAGAAAGAGGCAGCGGCTCAGCCAAGAAGCGCCTTTTGTACCCTGTCTCGGCTGAGCCACAACACCATAATTGTGTTGGCATTGATTGTAGTAGCTTTAGCCAGAACTCGAGAAGTCCTAGCTTCAAGAGTGTACAAAATAATGGCTACGATGGGAGGGAGAACTTGTCGTCGTACGCCGATACCCTTGGGGGAGAGATTTCTCCATGTTCAACCACTGATCTGTGGTGGTTAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

483

Amino Acids

54.19

Weight (kDa)

10.07

Isoelectric Point (pI)

56.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 337 - 447 9.2e-22 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1076
AccII CGCG 1 cut(s) 113
AccIII TCCGGA 1 cut(s) 51
AciI CCGC 5 cut(s) 165, 197, 1000, 1152, 1224
AclI AACGTT 1 cut(s) 357
AcoI YGGCCR 2 cut(s) 162, 741
AcsI RAATTY 1 cut(s) 820
AcuI CTGAAG 4 cut(s) 497, 672, 897, 1081
AcyI GRCGYC 1 cut(s) 453
AfaI GTAC 5 cut(s) 411, 941, 1250, 1344, 1385
AfiI CCNNNNNNNGG 3 cut(s) 689, 1027, 1258
AhdI GACNNNNNGTC 2 cut(s) 532, 1008
AjuI GAANNNNNNNTTGG 2 cut(s) 113, 145
Alw21I GWGCWC 2 cut(s) 304, 842
Alw26I GTCTC 1 cut(s) 1261
Alw44I GTGCAC 1 cut(s) 838
AlwNI CAGNNNCTG 2 cut(s) 98, 173
Ama87I CYCGRG 2 cut(s) 1205, 1318
Aor13HI TCCGGA 1 cut(s) 51
AoxI GGCC 7 cut(s) 162, 484, 668, 741, 827, 1158, 1193
ApaLI GTGCAC 1 cut(s) 838
ApeKI GCWGC 8 cut(s) 167, 176, 254, 614, 889, 1052, 1139, 1221
ApoI RAATTY 1 cut(s) 820
AspLEI GCGC 3 cut(s) 113, 115, 1242
AsuHPI GGTGA 2 cut(s) 572, 912
AsuNHI GCTAGC 1 cut(s) 892
AvaI CYCGRG 2 cut(s) 1205, 1318
BaeGI GKGCMC 1 cut(s) 842
BalI TGGCCA 1 cut(s) 743
BanI GGYRCC 1 cut(s) 1076
BanII GRGCYC 1 cut(s) 304
BauI CACGAG 1 cut(s) 835
Bbv12I GWGCWC 2 cut(s) 304, 842
BbvI GCAGC 8 cut(s) 179, 188, 241, 601, 901, 1039, 1126, 1233
BccI CCATC 5 cut(s) 691, 717, 729, 733, 1355
BceAI ACGGC 3 cut(s) 288, 1020, 1098
BcoDI GTCTC 1 cut(s) 1261
BfaI CTAG 5 cut(s) 240, 707, 728, 893, 1329
BfmI CTRYAG 2 cut(s) 99, 174
BfoI RGCGCY 1 cut(s) 1243
BglI GCCNNNNNGGC 1 cut(s) 197
BlpI GCTNAGC 4 cut(s) 138, 1175, 1228, 1263
BmeRI GACNNNNNGTC 2 cut(s) 532, 1008
BmeT110I CYCGRG 2 cut(s) 1205, 1318
BmiI GGNNCC 2 cut(s) 680, 1078
BmsI GCATC 4 cut(s) 94, 124, 688, 1007
BmtI GCTAGC 1 cut(s) 896
BoxI GACNNNNGTC 1 cut(s) 456
Bpu1102I GCTNAGC 4 cut(s) 138, 1175, 1228, 1263
BpuEI CTTGAG 1 cut(s) 299
Bsa29I ATCGAT 1 cut(s) 813
BsaHI GRCGYC 1 cut(s) 453
BsaJI CCNNGG 2 cut(s) 1161, 1396
BsaWI WCCGGW 1 cut(s) 51
BsaXI ACNNNNNCTCC 4 cut(s) 955, 985, 1361, 1391
Bsc4I CCNNNNNNNGG 3 cut(s) 689, 1027, 1258
Bse118I RCCGGY 1 cut(s) 455
Bse3DI GCAATG 2 cut(s) 906, 1113
BseAI TCCGGA 1 cut(s) 51
BseCI ATCGAT 1 cut(s) 813
BseDI CCNNGG 2 cut(s) 1161, 1396
BseGI GGATG 3 cut(s) 139, 700, 744
BseLI CCNNNNNNNGG 3 cut(s) 689, 1027, 1258
BseMI GCAATG 2 cut(s) 906, 1113
BseMII CTCAG 4 cut(s) 129, 663, 1242, 1254
BsePI GCGCGC 1 cut(s) 111
BseSI GKGCMC 1 cut(s) 842
BseXI GCAGC 8 cut(s) 179, 188, 241, 601, 901, 1039, 1126, 1233
BsgI GTGCAG 2 cut(s) 911, 1038
Bsh1236I CGCG 1 cut(s) 113
Bsh1285I CGRYCG 2 cut(s) 460, 1172
BshFI GGCC 7 cut(s) 164, 486, 670, 743, 829, 1160, 1195
BshNI GGYRCC 1 cut(s) 1076
BshVI ATCGAT 1 cut(s) 813
BsiEI CGRYCG 2 cut(s) 460, 1172
BsiHKAI GWGCWC 2 cut(s) 304, 842
BsiHKCI CYCGRG 2 cut(s) 1205, 1318
BsiSI CCGG 2 cut(s) 52, 456
BsiWI CGTACG 1 cut(s) 1383
BslFI GGGAC 2 cut(s) 587, 839
BslI CCNNNNNNNGG 3 cut(s) 689, 1027, 1258
BsmAI GTCTC 1 cut(s) 1261
BsmFI GGGAC 2 cut(s) 587, 839
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 7 cut(s) 164, 486, 670, 743, 829, 1160, 1195
BsoBI CYCGRG 2 cut(s) 1205, 1318
Bsp1286I GDGCHC 2 cut(s) 304, 842
Bsp13I TCCGGA 1 cut(s) 51
Bsp1407I TGTACA 1 cut(s) 1342
Bsp143I GATC 5 cut(s) 415, 434, 810, 1169, 1429
Bsp1720I GCTNAGC 4 cut(s) 138, 1175, 1228, 1263
BspACI CCGC 5 cut(s) 165, 197, 1000, 1152, 1224
BspANI GGCC 7 cut(s) 164, 486, 670, 743, 829, 1160, 1195
BspCNI CTCAG 4 cut(s) 130, 664, 1241, 1255
BspDI ATCGAT 1 cut(s) 813
BspEI TCCGGA 1 cut(s) 51
BspFNI CGCG 1 cut(s) 113
BspLI GGNNCC 2 cut(s) 680, 1078
BspMAI CTGCAG 1 cut(s) 178
BspOI GCTAGC 1 cut(s) 896
BspT107I GGYRCC 1 cut(s) 1076
BsrDI GCAATG 2 cut(s) 906, 1113
BsrFI RCCGGY 1 cut(s) 455
BsrGI TGTACA 1 cut(s) 1342
BssAI RCCGGY 1 cut(s) 455
BssECI CCNNGG 2 cut(s) 1161, 1396
BssHII GCGCGC 1 cut(s) 111
BssMI GATC 5 cut(s) 415, 434, 810, 1169, 1429
BssNI GRCGYC 1 cut(s) 453
BssSI CACGAG 1 cut(s) 835
BssT1I CCWWGG 2 cut(s) 1161, 1396
Bst2BI CACGAG 1 cut(s) 835
Bst4CI ACNGT 4 cut(s) 94, 100, 805, 939
Bst6I CTCTTC 1 cut(s) 705
BstACI GRCGYC 1 cut(s) 453
BstAPI GCANNNNNTGC 1 cut(s) 173
BstAUI TGTACA 1 cut(s) 1342
BstC8I GCNNGC 8 cut(s) 109, 113, 150, 369, 392, 396, 894, 960
BstDEI CTNAG 5 cut(s) 138, 672, 1175, 1228, 1263
BstF5I GGATG 3 cut(s) 139, 700, 744
BstFNI CGCG 1 cut(s) 113
BstH2I RGCGCY 1 cut(s) 1243
BstHHI GCGC 3 cut(s) 113, 115, 1242
BstKTI GATC 5 cut(s) 418, 437, 813, 1172, 1432
BstMAI GTCTC 1 cut(s) 1261
BstMBI GATC 5 cut(s) 415, 434, 810, 1169, 1429
BstMCI CGRYCG 2 cut(s) 460, 1172
BstNSI RCATGY 4 cut(s) 71, 152, 311, 394
BstPAI GACNNNNGTC 1 cut(s) 456
BstSFI CTRYAG 2 cut(s) 99, 174
BstSLI GKGCMC 1 cut(s) 842
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 8 cut(s) 179, 188, 241, 601, 901, 1039, 1126, 1233
Bsu15I ATCGAT 1 cut(s) 813
BsuRI GGCC 7 cut(s) 164, 486, 670, 743, 829, 1160, 1195
BsuTUI ATCGAT 1 cut(s) 813
BtsCI GGATG 3 cut(s) 139, 700, 744
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 4 cut(s) 99, 186, 380, 1425
Cac8I GCNNGC 8 cut(s) 109, 113, 150, 369, 392, 396, 894, 960
CaiI CAGNNNCTG 2 cut(s) 98, 173
CfoI GCGC 3 cut(s) 113, 115, 1242
Cfr10I RCCGGY 1 cut(s) 455
ClaI ATCGAT 1 cut(s) 813
CseI GACGC 1 cut(s) 461
Csp6I GTAC 5 cut(s) 410, 940, 1249, 1343, 1384
CspCI CAANNNNNGTGG 2 cut(s) 141, 176
CviQI GTAC 5 cut(s) 410, 940, 1249, 1343, 1384
DdeI CTNAG 5 cut(s) 138, 672, 1175, 1228, 1263
DpnI GATC 5 cut(s) 417, 436, 812, 1171, 1431
DpnII GATC 5 cut(s) 415, 434, 810, 1169, 1429
DraI TTTAAA 1 cut(s) 621
DriI GACNNNNNGTC 2 cut(s) 532, 1008
EaeI YGGCCR 2 cut(s) 162, 741
Eam1104I CTCTTC 1 cut(s) 705
Eam1105I GACNNNNNGTC 2 cut(s) 532, 1008
EarI CTCTTC 1 cut(s) 705
Ecl136II GAGCTC 1 cut(s) 302
Eco130I CCWWGG 2 cut(s) 1161, 1396
Eco147I AGGCCT 4 cut(s) 486, 670, 829, 1195
Eco24I GRGCYC 1 cut(s) 304
Eco32I GATATC 1 cut(s) 509
Eco53kI GAGCTC 1 cut(s) 302
Eco57I CTGAAG 4 cut(s) 497, 672, 897, 1081
Eco88I CYCGRG 2 cut(s) 1205, 1318
EcoICRI GAGCTC 1 cut(s) 302
EcoRI GAATTC 1 cut(s) 820
EcoRV GATATC 1 cut(s) 509
EcoT14I CCWWGG 2 cut(s) 1161, 1396
EcoT22I ATGCAT 1 cut(s) 392
EcoT38I GRGCYC 1 cut(s) 304
ErhI CCWWGG 2 cut(s) 1161, 1396
FalI AAGNNNNNCTT 2 cut(s) 757, 789
FaqI GGGAC 2 cut(s) 587, 839
FokI GGATG 3 cut(s) 146, 707, 751
FriOI GRGCYC 1 cut(s) 304
FspBI CTAG 5 cut(s) 240, 707, 728, 893, 1329
GlaI GCGC 3 cut(s) 112, 114, 1241
HaeII RGCGCY 1 cut(s) 1243
HaeIII GGCC 7 cut(s) 164, 486, 670, 743, 829, 1160, 1195
HapII CCGG 2 cut(s) 52, 456
HgaI GACGC 1 cut(s) 461
HhaI GCGC 3 cut(s) 113, 115, 1242
Hin1I GRCGYC 1 cut(s) 453
Hin6I GCGC 3 cut(s) 111, 113, 1240
HinP1I GCGC 3 cut(s) 111, 113, 1240
HindIII AAGCTT 3 cut(s) 329, 336, 1066
HinfI GANTC 5 cut(s) 121, 500, 946, 1009, 1148
HpaII CCGG 2 cut(s) 52, 456
HphI GGTGA 2 cut(s) 572, 912
Hpy166II GTNNAC 2 cut(s) 840, 1343
Hpy188I TCNGA 3 cut(s) 281, 660, 993
Hpy188III TCNNGA 7 cut(s) 52, 504, 515, 524, 1119, 1320, 1336
Hpy8I GTNNAC 2 cut(s) 840, 1343
Hpy99I CGWCG 1 cut(s) 1384
HpyAV CCTTC 5 cut(s) 33, 497, 840, 984, 1206
HpyCH4III ACNGT 4 cut(s) 94, 100, 805, 939
HpyCH4IV ACGT 2 cut(s) 357, 625
HpyF3I CTNAG 5 cut(s) 138, 672, 1175, 1228, 1263
HpySE526I ACGT 2 cut(s) 357, 625
Hsp92I GRCGYC 1 cut(s) 453
HspAI GCGC 3 cut(s) 111, 113, 1240
Kpn2I TCCGGA 1 cut(s) 51
Kzo9I GATC 5 cut(s) 415, 434, 810, 1169, 1429
Lsp1109I GCAGC 8 cut(s) 179, 188, 241, 601, 901, 1039, 1126, 1233
LweI GCATC 4 cut(s) 94, 124, 688, 1007
MaeI CTAG 5 cut(s) 240, 707, 728, 893, 1329
MaeII ACGT 2 cut(s) 357, 625
MaeIII GTNAC 4 cut(s) 94, 284, 524, 867
MalI GATC 5 cut(s) 417, 436, 812, 1171, 1431
MboI GATC 5 cut(s) 415, 434, 810, 1169, 1429
MboII GAAGA 5 cut(s) 34, 503, 678, 722, 837
MfeI CAATTG 2 cut(s) 153, 777
MhlI GDGCHC 2 cut(s) 304, 842
MlsI TGGCCA 1 cut(s) 743
MluCI AATT 7 cut(s) 153, 752, 777, 820, 858, 1129, 1279
MluNI TGGCCA 1 cut(s) 743
MlyI GAGTC 1 cut(s) 1018
Mox20I TGGCCA 1 cut(s) 743
Mph1103I ATGCAT 1 cut(s) 392
MroI TCCGGA 1 cut(s) 51
MscI TGGCCA 1 cut(s) 743
MseI TTAA 2 cut(s) 620, 1442
MslI CAYNNNNRTG 2 cut(s) 385, 1281
Msp20I TGGCCA 1 cut(s) 743
MspA1I CMGCKG 2 cut(s) 1052, 1224
MspI CCGG 2 cut(s) 52, 456
MunI CAATTG 2 cut(s) 153, 777
Mva1269I GAATGC 1 cut(s) 275
MvnI CGCG 1 cut(s) 113
NdeII GATC 5 cut(s) 415, 434, 810, 1169, 1429
NheI GCTAGC 1 cut(s) 892
NlaIV GGNNCC 2 cut(s) 680, 1078
NmeAIII GCCGAG 2 cut(s) 794, 1238
NmuCI GTSAC 3 cut(s) 94, 524, 867
NsiI ATGCAT 1 cut(s) 392
NspI RCATGY 4 cut(s) 71, 152, 311, 394
PaeI GCATGC 2 cut(s) 152, 394
PaeR7I CTCGAG 1 cut(s) 1318
PauI GCGCGC 1 cut(s) 111
PceI AGGCCT 4 cut(s) 486, 670, 829, 1195
PctI GAATGC 1 cut(s) 275
PfeI GAWTC 4 cut(s) 121, 500, 946, 1148
Pfl23II CGTACG 1 cut(s) 1383
Ple19I CGATCG 1 cut(s) 1172
PleI GAGTC 1 cut(s) 1017
PpsI GAGTC 1 cut(s) 1017
PshAI GACNNNNGTC 1 cut(s) 456
Psp124BI GAGCTC 1 cut(s) 304
Psp1406I AACGTT 1 cut(s) 357
PspLI CGTACG 1 cut(s) 1383
PspN4I GGNNCC 2 cut(s) 680, 1078
PsrI GAACNNNNNNTAC 2 cut(s) 195, 227
PstI CTGCAG 1 cut(s) 178
PstNI CAGNNNCTG 2 cut(s) 98, 173
PteI GCGCGC 1 cut(s) 111
PvuI CGATCG 1 cut(s) 1172
PvuII CAGCTG 1 cut(s) 1052
RsaI GTAC 5 cut(s) 411, 941, 1250, 1344, 1385
RsaNI GTAC 5 cut(s) 410, 940, 1249, 1343, 1384
RseI CAYNNNNRTG 2 cut(s) 385, 1281
SacI GAGCTC 1 cut(s) 304
SaqAI TTAA 2 cut(s) 620, 1442
Sau3AI GATC 5 cut(s) 415, 434, 810, 1169, 1429
SchI GAGTC 1 cut(s) 1018
SduI GDGCHC 2 cut(s) 304, 842
SfaNI GCATC 4 cut(s) 94, 124, 688, 1007
SfcI CTRYAG 2 cut(s) 99, 174
Sfr274I CTCGAG 1 cut(s) 1318
SlaI CTCGAG 1 cut(s) 1318
SmiMI CAYNNNNRTG 2 cut(s) 385, 1281
SmlI CTYRAG 2 cut(s) 314, 1318
SmoI CTYRAG 2 cut(s) 314, 1318
SphI GCATGC 2 cut(s) 152, 394
Sse9I AATT 7 cut(s) 153, 752, 777, 820, 858, 1129, 1279
SseBI AGGCCT 4 cut(s) 486, 670, 829, 1195
SsiI CCGC 5 cut(s) 165, 197, 1000, 1152, 1224
SspMI CTAG 5 cut(s) 240, 707, 728, 893, 1329
SstI GAGCTC 1 cut(s) 304
StuI AGGCCT 4 cut(s) 486, 670, 829, 1195
StyI CCWWGG 2 cut(s) 1161, 1396
TaaI ACNGT 4 cut(s) 94, 100, 805, 939
TaiI ACGT 2 cut(s) 360, 628
TaqI TCGA 5 cut(s) 406, 460, 813, 1168, 1319
TaqII GACCGA 1 cut(s) 241
TasI AATT 7 cut(s) 153, 752, 777, 820, 858, 1129, 1279
TatI WGTACW 3 cut(s) 409, 939, 1342
TauI GCSGC 3 cut(s) 167, 200, 1227
TfiI GAWTC 4 cut(s) 121, 500, 946, 1148
Tru1I TTAA 2 cut(s) 620, 1442
Tru9I TTAA 2 cut(s) 620, 1442
TscAI CASTG 4 cut(s) 99, 186, 387, 1432
TseFI GTSAC 3 cut(s) 94, 524, 867
TseI GCWGC 8 cut(s) 167, 176, 254, 614, 889, 1052, 1139, 1221
Tsp45I GTSAC 3 cut(s) 94, 524, 867
TspDTI ATGAA 5 cut(s) 17, 23, 852, 959, 1035
TspRI CASTG 4 cut(s) 99, 186, 387, 1432
VneI GTGCAC 1 cut(s) 838
XapI RAATTY 1 cut(s) 820
XceI RCATGY 4 cut(s) 71, 152, 311, 394
XcmI CCANNNNNNNNNTGG 1 cut(s) 1283
XhoI CTCGAG 1 cut(s) 1318
XspI CTAG 5 cut(s) 240, 707, 728, 893, 1329
Zsp2I ATGCAT 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.