Rroxscaffold_3G00267450

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001398
Physical Location & Seq
Forward (+)
60487759 .. 60489709
1951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267450.1

Sequence Viewer

Length: 1461 bp
ATGGGAAAGAAAAGTGGTAGTACTTCATGGTTGACTATAGTCAAAAGAGCCTTCAGGTCTCCGTCCAAGGATCATGACCAAAAGAGCAGCCGAAGAAGAGAAGACCATGAACCAGAAGAGGAAGAAAAGAAGAGAGAGAAGAGAAGATGGCTATTCCGAAAGCCTACTACACATGTTCAACAATGTGTAGCAACAAAGACAGAAACAACCATAGCTGTGACTCCTGTTTTGGCTGCTGAACAAAAACATGCAATTGCTGTGGCCGTAGCCACTGCAGCTGCGGCCGAAGCAGCTGTTGCAACTGCCCAAGCGGCAGTAGAAATTGTTCGTCTCACTAGGCCGAACAATTTTGTCAAAGAGCATTATGCTGCTACACTCATTCAAACAGCTTTCAGAGGTTACTTAGCGAGGAGAGCTCTGCGTGCGCTTAAGGGGCTTGTAAAGCTCCAAGCTTTAGTGAGAGGTCACAATGTTCGAAAGCAAGCAAAGTTGACACTGAAGTGCATGCAAGCTCTGGTTCGAGTTCAAGATCAGGTTCGGAACGAAAGAGCCAGGCTTTCACATGAAGGAGGCAGAAACTCCATGTTTTCCGAAACAGACATGAGCCTATGGGAGTGCAGATACCTTCAAGACATTCGAGACAGAAGGTCGATTTCTAGAGAGAAAGTGAGTGAAGGAGTTGACCAATTCTTGTTGCAAAATCAAAAGGAAGCTTCTTTAAAACGTGAAAAAGCACTTGCTTATGCTTTTTCTCACCAGATATGGAGGCCTAGAAGACACCCATCTGTAGGGGACGAAGCAGAGCTAGAAGAGAGAACAAAATGGCTTGATCGGTGGATGGCTACAAAAAATTGGGAAAATTACAGAGCATCAACCGACAAAAGAGACTCCATAAAAACTGTTGAAATGGACACTTCCAGGCCTTACATTTCATCCCAACACCAAAAACAGCCAAACCCAAATGCTGCAATGGCTTCACCTTTGCACAAATCACATTACAATAACATGAACATCTCTCTCAGCCACTCACATGCAACACCCTCTCCTTCAAAACCCAGATCGCTAAACGTGCGTTCAGCTAGTCCAAGATGCTCTTCAGCAGCACACACACCAAACTTGGGCTCTGCTTACTGTTTCAGGGGTGGTATGTGTAGATATGGAGCGGGCTCAAATGGGGTTACTAATAATGTCATTGGTTCAGTGCCTAATTACATGGCTGCAACCGAGTCTGCAAAGGCCAAGGCTCGATCTCTAAGTGCACCACGGTCAAGGCCATCGACACCAGATAGAGACCGGGTTGGTTCGGCCAAGAAACGGCTTTCGTACCCGGCCCCTGAGTCACATAGCTTGAGAAGTCCTAGCTTCAAGAGTGTGCAAAATGGGTACTATGGAAGGGGGAACTTGTCTACTTACACTGATAGCCTAGGGGAGAAATTTCTCCATGTTCAACCACTGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

486

Amino Acids

54.67

Weight (kDa)

10.24

Isoelectric Point (pI)

58.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 364 - 450 1.7e-19 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1163
AccI GTMKAC 1 cut(s) 1406
AciI CCGC 3 cut(s) 281, 311, 1163
AclWI GGATC 1 cut(s) 78
AcoI YGGCCR 3 cut(s) 261, 282, 1305
AcsI RAATTY 1 cut(s) 1433
AcuI CTGAAG 3 cut(s) 37, 518, 1080
AfaI GTAC 3 cut(s) 22, 1325, 1385
AfiI CCNNNNNNNGG 3 cut(s) 788, 1118, 1314
AflII CTTAAG 1 cut(s) 428
AflIII ACRYGT 1 cut(s) 172
AgsI TTSAA 8 cut(s) 179, 383, 527, 629, 905, 1050, 1366, 1448
AhdI GACNNNNNGTC 1 cut(s) 646
AjnI CCWGG 2 cut(s) 551, 917
AjuI GAANNNNNNNTTGG 2 cut(s) 1078, 1110
AleI CACNNNNGTG 1 cut(s) 499
Alw21I GWGCWC 2 cut(s) 418, 1261
Alw26I GTCTC 5 cut(s) 63, 335, 633, 879, 1284
Alw44I GTGCAC 1 cut(s) 1257
AlwI GGATC 1 cut(s) 78
AoxI GGCC 9 cut(s) 261, 282, 338, 767, 920, 1236, 1271, 1305, 1329
ApaLI GTGCAC 1 cut(s) 1257
ApeKI GCWGC 9 cut(s) 87, 233, 275, 278, 290, 368, 965, 1100, 1217
ApoI RAATTY 1 cut(s) 1433
ArsI GACNNNNNNTTYG 2 cut(s) 211, 243
Asp700I GAANNNNTTC 1 cut(s) 324
AspA2I CCTAGG 1 cut(s) 1423
AspLEI GCGC 1 cut(s) 427
AspS9I GGNCC 1 cut(s) 1330
AsuC2I CCSGG 2 cut(s) 1295, 1328
AsuHPI GGTGA 2 cut(s) 746, 969
AsuII TTCGAA 1 cut(s) 475
AvrII CCTAGG 1 cut(s) 1423
BaeGI GKGCMC 1 cut(s) 1261
BanII GRGCYC 3 cut(s) 418, 1124, 1169
BbsI GAAGAC 2 cut(s) 108, 781
Bbv12I GWGCWC 2 cut(s) 418, 1261
BbvI GCAGC 9 cut(s) 99, 220, 265, 287, 302, 355, 952, 1112, 1204
BccI CCATC 4 cut(s) 141, 790, 832, 1282
BceAI ACGGC 2 cut(s) 248, 1331
BciT130I CCWGG 2 cut(s) 553, 919
BcnI CCSGG 2 cut(s) 1295, 1328
BcoDI GTCTC 5 cut(s) 63, 335, 633, 879, 1284
BfaI CTAG 7 cut(s) 336, 657, 771, 806, 1080, 1359, 1424
BfmI CTRYAG 3 cut(s) 36, 273, 786
BfrI CTTAAG 1 cut(s) 428
BglI GCCNNNNNGGC 1 cut(s) 311
BlnI CCTAGG 1 cut(s) 1423
BmcAI AGTACT 1 cut(s) 22
Bme1390I CCNGG 4 cut(s) 553, 919, 1295, 1328
BmeRI GACNNNNNGTC 1 cut(s) 646
BmgT120I GGNCC 1 cut(s) 1330
BmiI GGNNCC 1 cut(s) 1332
BmrFI CCNGG 4 cut(s) 553, 919, 1295, 1328
BmsI GCATC 2 cut(s) 878, 1079
BoxI GACNNNNGTC 1 cut(s) 38
BpiI GAAGAC 2 cut(s) 108, 781
BplI GAGNNNNNCTC 2 cut(s) 400, 432
Bpu14I TTCGAA 1 cut(s) 475
BpuEI CTTGAG 1 cut(s) 1369
BpuMI CCSGG 2 cut(s) 1295, 1328
BsaI GGTCTC 2 cut(s) 63, 1284
BsaJI CCNNGG 4 cut(s) 66, 1239, 1262, 1423
BsaXI ACNNNNNCTCC 2 cut(s) 1027, 1057
Bsc4I CCNNNNNNNGG 3 cut(s) 788, 1118, 1314
Bse3DI GCAATG 1 cut(s) 975
BseBI CCWGG 2 cut(s) 553, 919
BseDI CCNNGG 4 cut(s) 66, 1239, 1262, 1423
BseGI GGATG 2 cut(s) 843, 932
BseLI CCNNNNNNNGG 3 cut(s) 788, 1118, 1314
BseMI GCAATG 1 cut(s) 975
BseMII CTCAG 2 cut(s) 1033, 1326
BseRI GAGGAG 1 cut(s) 424
BseSI GKGCMC 1 cut(s) 1261
BseX3I CGGCCG 1 cut(s) 282
BseXI GCAGC 9 cut(s) 99, 220, 265, 287, 302, 355, 952, 1112, 1204
BsgI GTGCAG 1 cut(s) 637
Bsh1285I CGRYCG 1 cut(s) 285
BshFI GGCC 9 cut(s) 263, 284, 340, 769, 922, 1238, 1273, 1307, 1331
BsiEI CGRYCG 1 cut(s) 285
BsiHKAI GWGCWC 2 cut(s) 418, 1261
BsiSI CCGG 2 cut(s) 1294, 1328
BslFI GGGAC 1 cut(s) 806
BslI CCNNNNNNNGG 3 cut(s) 788, 1118, 1314
BsmAI GTCTC 5 cut(s) 63, 335, 633, 879, 1284
BsmBI CGTCTC 1 cut(s) 335
BsmFI GGGAC 1 cut(s) 806
BsnI GGCC 9 cut(s) 263, 284, 340, 769, 922, 1238, 1273, 1307, 1331
Bso31I GGTCTC 2 cut(s) 63, 1284
Bsp119I TTCGAA 1 cut(s) 475
Bsp1286I GDGCHC 4 cut(s) 418, 1124, 1169, 1261
Bsp143I GATC 5 cut(s) 70, 529, 829, 1058, 1247
BspACI CCGC 3 cut(s) 281, 311, 1163
BspANI GGCC 9 cut(s) 263, 284, 340, 769, 922, 1238, 1273, 1307, 1331
BspCNI CTCAG 2 cut(s) 1032, 1327
BspHI TCATGA 1 cut(s) 73
BspLI GGNNCC 1 cut(s) 1332
BspMAI CTGCAG 1 cut(s) 277
BspPI GGATC 1 cut(s) 78
BspQI GCTCTTC 1 cut(s) 1099
BspT104I TTCGAA 1 cut(s) 475
BspTI CTTAAG 1 cut(s) 428
BspTNI GGTCTC 2 cut(s) 63, 1284
BsrBI CCGCTC 1 cut(s) 1163
BsrDI GCAATG 1 cut(s) 975
BssECI CCNNGG 4 cut(s) 66, 1239, 1262, 1423
BssMI GATC 5 cut(s) 70, 529, 829, 1058, 1247
BssT1I CCWWGG 3 cut(s) 66, 1239, 1423
Bst2UI CCWGG 2 cut(s) 553, 919
Bst4CI ACNGT 3 cut(s) 901, 1133, 1266
Bst6I CTCTTC 6 cut(s) 91, 111, 125, 134, 804, 1099
BstAFI CTTAAG 1 cut(s) 428
BstAPI GCANNNNNTGC 1 cut(s) 296
BstBI TTCGAA 1 cut(s) 475
BstC8I GCNNGC 5 cut(s) 423, 483, 506, 510, 1165
BstDEI CTNAG 4 cut(s) 403, 1019, 1253, 1335
BstDSI CCRYGG 1 cut(s) 1262
BstF5I GGATG 2 cut(s) 843, 932
BstHHI GCGC 1 cut(s) 427
BstKTI GATC 5 cut(s) 73, 532, 832, 1061, 1250
BstMAI GTCTC 5 cut(s) 63, 335, 633, 879, 1284
BstMBI GATC 5 cut(s) 70, 529, 829, 1058, 1247
BstMCI CGRYCG 1 cut(s) 285
BstNI CCWGG 2 cut(s) 553, 919
BstNSI RCATGY 4 cut(s) 176, 251, 508, 1034
BstPAI GACNNNNGTC 1 cut(s) 38
BstSCI CCNGG 4 cut(s) 551, 917, 1293, 1326
BstSFI CTRYAG 3 cut(s) 36, 273, 786
BstSLI GKGCMC 1 cut(s) 1261
BstV1I GCAGC 9 cut(s) 99, 220, 265, 287, 302, 355, 952, 1112, 1204
BstV2I GAAGAC 2 cut(s) 108, 781
BstZI CGGCCG 1 cut(s) 282
BsuRI GGCC 9 cut(s) 263, 284, 340, 769, 922, 1238, 1273, 1307, 1331
BtgI CCRYGG 1 cut(s) 1262
BtsCI GGATG 2 cut(s) 843, 932
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 5 cut(s) 270, 494, 1206, 1413, 1451
Cac8I GCNNGC 5 cut(s) 423, 483, 506, 510, 1165
CciI TCATGA 1 cut(s) 73
CfoI GCGC 1 cut(s) 427
Cfr13I GGNCC 1 cut(s) 1330
Csp6I GTAC 3 cut(s) 21, 1324, 1384
CspCI CAANNNNNGTGG 2 cut(s) 240, 275
CviQI GTAC 3 cut(s) 21, 1324, 1384
DdeI CTNAG 4 cut(s) 403, 1019, 1253, 1335
DpnI GATC 5 cut(s) 72, 531, 831, 1060, 1249
DpnII GATC 5 cut(s) 70, 529, 829, 1058, 1247
DraI TTTAAA 1 cut(s) 720
DriI GACNNNNNGTC 1 cut(s) 646
EaeI YGGCCR 3 cut(s) 261, 282, 1305
EagI CGGCCG 1 cut(s) 282
Eam1104I CTCTTC 6 cut(s) 91, 111, 125, 134, 804, 1099
Eam1105I GACNNNNNGTC 1 cut(s) 646
EarI CTCTTC 6 cut(s) 91, 111, 125, 134, 804, 1099
Ecl136II GAGCTC 1 cut(s) 416
EclXI CGGCCG 1 cut(s) 282
Eco130I CCWWGG 3 cut(s) 66, 1239, 1423
Eco147I AGGCCT 2 cut(s) 769, 922
Eco24I GRGCYC 3 cut(s) 418, 1124, 1169
Eco31I GGTCTC 2 cut(s) 63, 1284
Eco52I CGGCCG 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 416
Eco57I CTGAAG 3 cut(s) 37, 518, 1080
EcoICRI GAGCTC 1 cut(s) 416
EcoRII CCWGG 2 cut(s) 551, 917
EcoT14I CCWWGG 3 cut(s) 66, 1239, 1423
EcoT38I GRGCYC 3 cut(s) 418, 1124, 1169
ErhI CCWWGG 3 cut(s) 66, 1239, 1423
Esp3I CGTCTC 1 cut(s) 335
FalI AAGNNNNNCTT 2 cut(s) 1078, 1110
FaqI GGGAC 1 cut(s) 806
FauI CCCGC 1 cut(s) 1156
FblI GTMKAC 1 cut(s) 1406
FokI GGATG 2 cut(s) 850, 919
FriOI GRGCYC 3 cut(s) 418, 1124, 1169
FspBI CTAG 7 cut(s) 336, 657, 771, 806, 1080, 1359, 1424
GlaI GCGC 1 cut(s) 426
HaeIII GGCC 9 cut(s) 263, 284, 340, 769, 922, 1238, 1273, 1307, 1331
HapII CCGG 2 cut(s) 1294, 1328
HhaI GCGC 1 cut(s) 427
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HincII GTYRAC 3 cut(s) 33, 492, 682
HindII GTYRAC 3 cut(s) 33, 492, 682
HindIII AAGCTT 2 cut(s) 450, 711
HinfI GANTC 4 cut(s) 220, 887, 1226, 1337
HpaII CCGG 2 cut(s) 1294, 1328
HphI GGTGA 2 cut(s) 746, 969
Hpy166II GTNNAC 5 cut(s) 33, 492, 682, 1259, 1407
Hpy188I TCNGA 4 cut(s) 158, 395, 540, 592
Hpy188III TCNNGA 6 cut(s) 74, 527, 629, 638, 657, 1366
Hpy8I GTNNAC 5 cut(s) 33, 492, 682, 1259, 1407
HpyAV CCTTC 7 cut(s) 61, 560, 635, 639, 668, 1056, 1386
HpyCH4III ACNGT 3 cut(s) 901, 1133, 1266
HpyCH4IV ACGT 2 cut(s) 724, 1068
HpyF3I CTNAG 4 cut(s) 403, 1019, 1253, 1335
HpySE526I ACGT 2 cut(s) 724, 1068
HspAI GCGC 1 cut(s) 425
Kzo9I GATC 5 cut(s) 70, 529, 829, 1058, 1247
LguI GCTCTTC 1 cut(s) 1099
LmnI GCTCC 2 cut(s) 450, 1160
Lsp1109I GCAGC 9 cut(s) 99, 220, 265, 287, 302, 355, 952, 1112, 1204
LweI GCATC 2 cut(s) 878, 1079
MaeI CTAG 7 cut(s) 336, 657, 771, 806, 1080, 1359, 1424
MaeII ACGT 2 cut(s) 724, 1068
MaeIII GTNAC 5 cut(s) 217, 398, 464, 1177, 1338
MalI GATC 5 cut(s) 72, 531, 831, 1060, 1249
MbiI CCGCTC 1 cut(s) 1163
MboI GATC 5 cut(s) 70, 529, 829, 1058, 1247
MfeI CAATTG 1 cut(s) 252
MhlI GDGCHC 4 cut(s) 418, 1124, 1169, 1261
MluCI AATT 8 cut(s) 252, 321, 346, 686, 850, 859, 1207, 1433
MlyI GAGTC 4 cut(s) 214, 881, 1235, 1346
MnlI CCTC 7 cut(s) 112, 389, 402, 455, 563, 759, 1051
MroXI GAANNNNTTC 1 cut(s) 324
MseI TTAA 3 cut(s) 429, 719, 1459
MslI CAYNNNNRTG 3 cut(s) 215, 499, 1029
MspA1I CMGCKG 2 cut(s) 278, 293
MspCI CTTAAG 1 cut(s) 428
MspI CCGG 2 cut(s) 1294, 1328
MspR9I CCNGG 4 cut(s) 553, 919, 1295, 1328
MunI CAATTG 1 cut(s) 252
MvaI CCWGG 2 cut(s) 553, 919
NciI CCSGG 2 cut(s) 1295, 1328
NdeII GATC 5 cut(s) 70, 529, 829, 1058, 1247
NlaIV GGNNCC 1 cut(s) 1332
NmuCI GTSAC 3 cut(s) 217, 464, 1338
NspI RCATGY 4 cut(s) 176, 251, 508, 1034
NspV TTCGAA 1 cut(s) 475
OliI CACNNNNGTG 1 cut(s) 499
PaeI GCATGC 1 cut(s) 508
PagI TCATGA 1 cut(s) 73
PceI AGGCCT 2 cut(s) 769, 922
PciI ACATGT 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 1099
PdmI GAANNNNTTC 1 cut(s) 324
PleI GAGTC 4 cut(s) 214, 881, 1234, 1345
PpsI GAGTC 4 cut(s) 214, 881, 1234, 1345
PscI ACATGT 1 cut(s) 172
PshAI GACNNNNGTC 1 cut(s) 38
Psp124BI GAGCTC 1 cut(s) 418
Psp6I CCWGG 2 cut(s) 551, 917
PspGI CCWGG 2 cut(s) 551, 917
PspN4I GGNNCC 1 cut(s) 1332
PspPI GGNCC 1 cut(s) 1330
PsrI GAACNNNNNNTAC 2 cut(s) 309, 341
PstI CTGCAG 1 cut(s) 277
PvuII CAGCTG 2 cut(s) 278, 293
RsaI GTAC 3 cut(s) 22, 1325, 1385
RsaNI GTAC 3 cut(s) 21, 1324, 1384
RseI CAYNNNNRTG 3 cut(s) 215, 499, 1029
SacI GAGCTC 1 cut(s) 418
SapI GCTCTTC 1 cut(s) 1099
SaqAI TTAA 3 cut(s) 429, 719, 1459
Sau3AI GATC 5 cut(s) 70, 529, 829, 1058, 1247
Sau96I GGNCC 1 cut(s) 1330
ScaI AGTACT 1 cut(s) 22
SchI GAGTC 4 cut(s) 214, 881, 1235, 1346
ScrFI CCNGG 4 cut(s) 553, 919, 1295, 1328
SduI GDGCHC 4 cut(s) 418, 1124, 1169, 1261
SfaNI GCATC 2 cut(s) 878, 1079
SfcI CTRYAG 3 cut(s) 36, 273, 786
SfuI TTCGAA 1 cut(s) 475
SmiMI CAYNNNNRTG 3 cut(s) 215, 499, 1029
SmlI CTYRAG 2 cut(s) 428, 1348
SmoI CTYRAG 2 cut(s) 428, 1348
SphI GCATGC 1 cut(s) 508
Sse9I AATT 8 cut(s) 252, 321, 346, 686, 850, 859, 1207, 1433
SseBI AGGCCT 2 cut(s) 769, 922
SsiI CCGC 3 cut(s) 281, 311, 1163
SspMI CTAG 7 cut(s) 336, 657, 771, 806, 1080, 1359, 1424
SstI GAGCTC 1 cut(s) 418
StuI AGGCCT 2 cut(s) 769, 922
StyD4I CCNGG 4 cut(s) 551, 917, 1293, 1326
StyI CCWWGG 3 cut(s) 66, 1239, 1423
TaaI ACNGT 3 cut(s) 901, 1133, 1266
TaiI ACGT 2 cut(s) 727, 1071
TaqI TCGA 6 cut(s) 475, 520, 637, 650, 1246, 1277
TasI AATT 8 cut(s) 252, 321, 346, 686, 850, 859, 1207, 1433
TatI WGTACW 1 cut(s) 20
TauI GCSGC 2 cut(s) 284, 314
Tru1I TTAA 3 cut(s) 429, 719, 1459
Tru9I TTAA 3 cut(s) 429, 719, 1459
TscAI CASTG 5 cut(s) 277, 501, 1206, 1420, 1458
TseFI GTSAC 3 cut(s) 217, 464, 1338
TseI GCWGC 9 cut(s) 87, 233, 275, 278, 290, 368, 965, 1100, 1217
Tsp45I GTSAC 3 cut(s) 217, 464, 1338
TspDTI ATGAA 5 cut(s) 15, 123, 579, 921, 1022
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 5 cut(s) 277, 501, 1206, 1420, 1458
Vha464I CTTAAG 1 cut(s) 428
VneI GTGCAC 1 cut(s) 1257
XapI RAATTY 1 cut(s) 1433
XbaI TCTAGA 1 cut(s) 656
XceI RCATGY 4 cut(s) 176, 251, 508, 1034
XmaJI CCTAGG 1 cut(s) 1423
XmiI GTMKAC 1 cut(s) 1406
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 7 cut(s) 336, 657, 771, 806, 1080, 1359, 1424
ZrmI AGTACT 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.