Prupe.5G131700_v2.0.a1

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001398
Physical Location & Seq
Reverse (-)
12675538 .. 12678057
2520 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G131700.1

Sequence Viewer

Length: 1572 bp
ATGGGAAAGAAAAGTGGTACCTCATGGTTGACTATAGTCAAAAGGGCATTTAGGTCTCCAACTAAAGATAACGATCAGCAAAAGATCAGCAGAAGAAGAGATGAGCATGAACCAGAAGAGGAAGAAAAGAAGAGAGAAAAGAGAAGATGGCTTTTCCGAAAGCCAACTATTCAGGTTCAACAATGTGAAGCAAAGACAGTGACAATAAATGCAACAACCACACCTGTGAATCCTGTTTTGGTTGCTGAGCAAAGGCGTGCAATCGCTGTGGCCGCAGCCACTGCAGCTGCGGCCGAAGCAGCTGTTGCTACTGCCCAAGCGGCAGTAAAAGTTGTTCGGCTTACTAGGCCGAACAATTTTGCTAAAGAACAGTATGCTGCCACCCTCATTCAAACAGCATTCAGAGGTTACTTAGCAAGGAGAGCTCTACGTGCACTGAAGGGGCTGGTAAAGCTTCAAGCTTTAGTGAGAGGTCATAATGTAAGGAAGCAAGCAAAGCTGACACTGAAATGCATGCAAGCTCTGCTTCGTGTACAAGATCAGGTTCGCAATGAAAGATCAAGGCTTTCACATGATGGGGGTAGAAAGTCCATGTTTGCCGAAACAGACTTGCCCTTGTGGGAATCGAGATATCTTCATGACAGTCGTGACAGAAAGTCAATGTCAAGAGAGAAAAGTCGTGTTGGGGATGATTGGGATGAAGGGGTGGAAGCCATGTTGCAGAGTAGAAGGGAAGCTGCTTTAAAACGTGAAAAGGCTCTTGCTTATGCTTTCTCTCACCAGATATGGAAGCCTCGGAGGAACCCATCTGCAGGGGATGAAGCAGAGCTAGAAGAGAGAACAAAATGGCTTGACAGATGGATGGCCACAAAGCAATTTGAGCACAGCAGCAGGGCTTCAATTGACAAAAGGGACACCATAAAAACAGTTGAAATTGACACCAATTCAAGGCCTCACTCTTACTCTTACTCTGCCCCAAATGTTCGAATTTCGACATCACATTCACAACTTCAAAAACAGCCAGCCCCATATCCAATTGCTTCACCTTCACCCCTGCACAAATCGCACTACATTAATCATCTCTCACCTGCCACACCCTCTCCTTCAAAACCCAAATATCTGAAAGTCCGATCAGCAAGCCCAAGATGCCTGAAGGAGGAAAAGTATTGCTGCTCATCAGCAGCACACACACCAAACTTAGGCTCTAATTCTTATTGTTTCAGGGGTTCAATGTGTCGTTCTGCGATACCAAATTACATGGCTGCCACTGAATCTGCAAAGGCAAAGGCTCGATCTCTTAGTGCACCCAGAACAAGACCCTCGACACCCGAGAGAGAAAGAGGTGGTGGTTCAGCCAAGAAGAGACTTTTATATCCAGTCCCAGCTGAGGCACTGCAACATAACTCTGTTGGCATTGGTTGTAGTAGTTTCAGCCATAACTTGAGAAGTCCCAGCTTCAAGAGTGTACAAAATAATGGCTACTATGGAAGGGAGAATTTGTCTTCATATACTGATAGCCTAGGGGGAGAGATTTCTCCTTGTTCAACCACTGATCTAAGGTGGTTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

524

Amino Acids

58.89

Weight (kDa)

10.12

Isoelectric Point (pI)

64.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1096
Acc36I ACCTGC 1 cut(s) 1096
Acc65I GGTACC 1 cut(s) 17
AccB1I GGYRCC 1 cut(s) 17
AciI CCGC 3 cut(s) 273, 290, 320
AcoI YGGCCR 3 cut(s) 270, 291, 864
AcsI RAATTY 2 cut(s) 987, 1495
AcuI CTGAAG 2 cut(s) 458, 1172
AfaI GTAC 3 cut(s) 19, 534, 1467
AfiI CCNNNNNNNGG 5 cut(s) 812, 948, 1156, 1199, 1387
AhdI GACNNNNNGTC 1 cut(s) 655
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AleI CACNNNNGTG 1 cut(s) 224
Alw21I GWGCWC 4 cut(s) 427, 436, 885, 1306
Alw26I GTCTC 2 cut(s) 60, 1357
Alw44I GTGCAC 2 cut(s) 432, 1302
AlwNI CAGNNNCTG 1 cut(s) 281
Ama87I CYCGRG 1 cut(s) 1328
AoxI GGCC 5 cut(s) 270, 291, 347, 864, 950
ApaLI GTGCAC 2 cut(s) 432, 1302
ApoI RAATTY 2 cut(s) 987, 1495
AseI ATTAAT 1 cut(s) 1074
Asp718I GGTACC 1 cut(s) 17
AspA2I CCTAGG 1 cut(s) 1519
AsuHPI GGTGA 4 cut(s) 770, 1035, 1041, 1077
AsuII TTCGAA 1 cut(s) 985
AvaI CYCGRG 1 cut(s) 1328
AvrII CCTAGG 1 cut(s) 1519
BaeGI GKGCMC 2 cut(s) 436, 1306
BalI TGGCCA 1 cut(s) 866
BanI GGYRCC 1 cut(s) 17
BanII GRGCYC 1 cut(s) 427
BbsI GAAGAC 1 cut(s) 1494
Bbv12I GWGCWC 4 cut(s) 427, 436, 885, 1306
BbvCI CCTCAGC 1 cut(s) 1386
BccI CCATC 5 cut(s) 141, 569, 814, 852, 856
BcoDI GTCTC 2 cut(s) 60, 1357
BfaI CTAG 3 cut(s) 345, 830, 1520
BfmI CTRYAG 3 cut(s) 33, 282, 810
BfuAI ACCTGC 1 cut(s) 1096
BglI GCCNNNNNGGC 1 cut(s) 320
BlnI CCTAGG 1 cut(s) 1519
BlpI GCTNAGC 1 cut(s) 246
BmeRI GACNNNNNGTC 1 cut(s) 655
BmeT110I CYCGRG 1 cut(s) 1328
BmiI GGNNCC 2 cut(s) 19, 803
BmsI GCATC 1 cut(s) 1136
BoxI GACNNNNGTC 1 cut(s) 35
BpiI GAAGAC 1 cut(s) 1494
Bpu10I CCTNAGC 1 cut(s) 1386
Bpu1102I GCTNAGC 1 cut(s) 246
Bpu14I TTCGAA 1 cut(s) 985
BpuEI CTTGAG 1 cut(s) 1462
BsaAI YACGTR 1 cut(s) 431
BsaBI GATNNNNATC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 60
BsaJI CCNNGG 2 cut(s) 794, 1519
BsaXI ACNNNNNCTCC 6 cut(s) 1084, 1114, 1148, 1178, 1484, 1514
Bsc4I CCNNNNNNNGG 5 cut(s) 812, 948, 1156, 1199, 1387
Bse1I ACTGG 1 cut(s) 1376
Bse3DI GCAATG 1 cut(s) 556
Bse8I GATNNNNATC 1 cut(s) 72
BseDI CCNNGG 2 cut(s) 794, 1519
BseGI GGATG 4 cut(s) 694, 703, 823, 867
BseJI GATNNNNATC 1 cut(s) 72
BseLI CCNNNNNNNGG 5 cut(s) 812, 948, 1156, 1199, 1387
BseMI GCAATG 1 cut(s) 556
BseMII CTCAG 2 cut(s) 237, 1377
BseNI ACTGG 1 cut(s) 1376
BseSI GKGCMC 2 cut(s) 436, 1306
BseX3I CGGCCG 1 cut(s) 291
BseYI CCCAGC 2 cut(s) 1381, 1451
BsgI GTGCAG 1 cut(s) 1040
Bsh1285I CGRYCG 1 cut(s) 294
BshFI GGCC 5 cut(s) 272, 293, 349, 866, 952
BshNI GGYRCC 1 cut(s) 17
BsiEI CGRYCG 1 cut(s) 294
BsiHKAI GWGCWC 4 cut(s) 427, 436, 885, 1306
BsiHKCI CYCGRG 1 cut(s) 1328
BslFI GGGAC 3 cut(s) 926, 1364, 1434
BslI CCNNNNNNNGG 5 cut(s) 812, 948, 1156, 1199, 1387
BsmAI GTCTC 2 cut(s) 60, 1357
BsmFI GGGAC 3 cut(s) 926, 1364, 1434
BsmI GAATGC 1 cut(s) 398
BsnI GGCC 5 cut(s) 272, 293, 349, 866, 952
Bso31I GGTCTC 1 cut(s) 60
BsoBI CYCGRG 1 cut(s) 1328
Bsp119I TTCGAA 1 cut(s) 985
Bsp1286I GDGCHC 4 cut(s) 427, 436, 885, 1306
Bsp1407I TGTACA 2 cut(s) 532, 1465
Bsp143I GATC 7 cut(s) 73, 84, 538, 557, 1130, 1292, 1552
Bsp1720I GCTNAGC 1 cut(s) 246
BspACI CCGC 3 cut(s) 273, 290, 320
BspANI GGCC 5 cut(s) 272, 293, 349, 866, 952
BspCNI CTCAG 2 cut(s) 238, 1378
BspHI TCATGA 1 cut(s) 637
BspLI GGNNCC 2 cut(s) 19, 803
BspMAI CTGCAG 2 cut(s) 286, 814
BspMI ACCTGC 1 cut(s) 1096
BspT104I TTCGAA 1 cut(s) 985
BspT107I GGYRCC 1 cut(s) 17
BspTNI GGTCTC 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 556
BsrGI TGTACA 2 cut(s) 532, 1465
BsrI ACTGG 1 cut(s) 1376
BssECI CCNNGG 2 cut(s) 794, 1519
BssMI GATC 7 cut(s) 73, 84, 538, 557, 1130, 1292, 1552
BssT1I CCWWGG 1 cut(s) 1519
Bst4CI ACNGT 4 cut(s) 199, 372, 644, 928
Bst6I CTCTTC 5 cut(s) 91, 111, 125, 828, 1355
BstAPI GCANNNNNTGC 3 cut(s) 281, 305, 523
BstAUI TGTACA 2 cut(s) 532, 1465
BstBAI YACGTR 1 cut(s) 431
BstBI TTCGAA 1 cut(s) 985
BstC8I GCNNGC 6 cut(s) 258, 492, 515, 519, 1023, 1138
BstDEI CTNAG 6 cut(s) 246, 412, 1198, 1298, 1386, 1556
BstENI CCTNNNNNAGG 1 cut(s) 1154
BstF5I GGATG 4 cut(s) 694, 703, 823, 867
BstKTI GATC 7 cut(s) 76, 87, 541, 560, 1133, 1295, 1555
BstMAI GTCTC 2 cut(s) 60, 1357
BstMBI GATC 7 cut(s) 73, 84, 538, 557, 1130, 1292, 1552
BstMCI CGRYCG 1 cut(s) 294
BstNSI RCATGY 1 cut(s) 517
BstPAI GACNNNNGTC 1 cut(s) 35
BstSFI CTRYAG 3 cut(s) 33, 282, 810
BstSLI GKGCMC 2 cut(s) 436, 1306
BstV2I GAAGAC 1 cut(s) 1494
BstZI CGGCCG 1 cut(s) 291
BsuRI GGCC 5 cut(s) 272, 293, 349, 866, 952
BtsCI GGATG 4 cut(s) 694, 703, 823, 867
BtsI GCAGTG 2 cut(s) 279, 1391
BtsIMutI CAGTG 7 cut(s) 204, 279, 434, 503, 1266, 1391, 1548
BveI ACCTGC 1 cut(s) 1096
Cac8I GCNNGC 6 cut(s) 258, 492, 515, 519, 1023, 1138
CaiI CAGNNNCTG 1 cut(s) 281
CciI TCATGA 1 cut(s) 637
Csp6I GTAC 3 cut(s) 18, 533, 1466
CspCI CAANNNNNGTGG 2 cut(s) 249, 284
CviAII CATG 8 cut(s) 24, 107, 514, 572, 592, 638, 715, 1258
CviQI GTAC 3 cut(s) 18, 533, 1466
DdeI CTNAG 6 cut(s) 246, 412, 1198, 1298, 1386, 1556
DpnI GATC 7 cut(s) 75, 86, 540, 559, 1132, 1294, 1554
DpnII GATC 7 cut(s) 73, 84, 538, 557, 1130, 1292, 1552
DraI TTTAAA 1 cut(s) 744
DriI GACNNNNNGTC 1 cut(s) 655
EaeI YGGCCR 3 cut(s) 270, 291, 864
EagI CGGCCG 1 cut(s) 291
Eam1104I CTCTTC 5 cut(s) 91, 111, 125, 828, 1355
Eam1105I GACNNNNNGTC 1 cut(s) 655
EarI CTCTTC 5 cut(s) 91, 111, 125, 828, 1355
Ecl136II GAGCTC 1 cut(s) 425
EclXI CGGCCG 1 cut(s) 291
Eco130I CCWWGG 1 cut(s) 1519
Eco147I AGGCCT 1 cut(s) 952
Eco24I GRGCYC 1 cut(s) 427
Eco31I GGTCTC 1 cut(s) 60
Eco32I GATATC 1 cut(s) 632
Eco52I CGGCCG 1 cut(s) 291
Eco53kI GAGCTC 1 cut(s) 425
Eco57I CTGAAG 2 cut(s) 458, 1172
Eco88I CYCGRG 1 cut(s) 1328
EcoICRI GAGCTC 1 cut(s) 425
EcoNI CCTNNNNNAGG 1 cut(s) 1154
EcoRV GATATC 1 cut(s) 632
EcoT14I CCWWGG 1 cut(s) 1519
EcoT22I ATGCAT 1 cut(s) 515
EcoT38I GRGCYC 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 1519
FaeI CATG 8 cut(s) 27, 110, 517, 575, 595, 641, 718, 1261
FalI AAGNNNNNCTT 2 cut(s) 510, 542
FaqI GGGAC 3 cut(s) 926, 1364, 1434
FatI CATG 8 cut(s) 23, 106, 513, 571, 591, 637, 714, 1257
FokI GGATG 4 cut(s) 701, 710, 830, 874
FriOI GRGCYC 1 cut(s) 427
FspBI CTAG 3 cut(s) 345, 830, 1520
GsaI CCCAGC 2 cut(s) 1385, 1455
HaeIII GGCC 5 cut(s) 272, 293, 349, 866, 952
Hin1II CATG 8 cut(s) 27, 110, 517, 575, 595, 641, 718, 1261
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HindIII AAGCTT 2 cut(s) 452, 459
HinfI GANTC 3 cut(s) 229, 623, 1271
HphI GGTGA 4 cut(s) 770, 1035, 1041, 1077
Hpy166II GTNNAC 5 cut(s) 30, 434, 533, 1304, 1466
Hpy188I TCNGA 5 cut(s) 158, 404, 798, 1122, 1130
Hpy188III TCNNGA 5 cut(s) 627, 638, 647, 666, 1459
Hpy8I GTNNAC 5 cut(s) 30, 434, 533, 1304, 1466
HpyAV CCTTC 7 cut(s) 433, 695, 723, 1056, 1113, 1147, 1482
HpyCH4III ACNGT 4 cut(s) 199, 372, 644, 928
HpyCH4IV ACGT 2 cut(s) 430, 748
HpyF3I CTNAG 6 cut(s) 246, 412, 1198, 1298, 1386, 1556
HpySE526I ACGT 2 cut(s) 430, 748
Hsp92II CATG 8 cut(s) 27, 110, 517, 575, 595, 641, 718, 1261
KpnI GGTACC 1 cut(s) 21
Kzo9I GATC 7 cut(s) 73, 84, 538, 557, 1130, 1292, 1552
LweI GCATC 1 cut(s) 1136
MaeI CTAG 3 cut(s) 345, 830, 1520
MaeII ACGT 2 cut(s) 430, 748
MaeIII GTNAC 3 cut(s) 199, 407, 647
MalI GATC 7 cut(s) 75, 86, 540, 559, 1132, 1294, 1554
MboI GATC 7 cut(s) 73, 84, 538, 557, 1130, 1292, 1552
MfeI CAATTG 2 cut(s) 900, 1035
MhlI GDGCHC 4 cut(s) 427, 436, 885, 1306
MlsI TGGCCA 1 cut(s) 866
MluNI TGGCCA 1 cut(s) 866
MmeI TCCRAC 1 cut(s) 83
Mox20I TGGCCA 1 cut(s) 866
Mph1103I ATGCAT 1 cut(s) 515
MscI TGGCCA 1 cut(s) 866
MseI TTAA 2 cut(s) 743, 1074
MslI CAYNNNNRTG 2 cut(s) 224, 508
Msp20I TGGCCA 1 cut(s) 866
MspA1I CMGCKG 3 cut(s) 287, 302, 1385
MunI CAATTG 2 cut(s) 900, 1035
Mva1269I GAATGC 1 cut(s) 398
NdeII GATC 7 cut(s) 73, 84, 538, 557, 1130, 1292, 1552
NlaIII CATG 8 cut(s) 27, 110, 517, 575, 595, 641, 718, 1261
NlaIV GGNNCC 2 cut(s) 19, 803
NmuCI GTSAC 2 cut(s) 199, 647
NsiI ATGCAT 1 cut(s) 515
NspI RCATGY 1 cut(s) 517
NspV TTCGAA 1 cut(s) 985
OliI CACNNNNGTG 1 cut(s) 224
PaeI GCATGC 1 cut(s) 517
PagI TCATGA 1 cut(s) 637
PaqCI CACCTGC 1 cut(s) 1096
PceI AGGCCT 1 cut(s) 952
PctI GAATGC 1 cut(s) 398
PfeI GAWTC 3 cut(s) 229, 623, 1271
Ppu21I YACGTR 1 cut(s) 431
PshAI GACNNNNGTC 1 cut(s) 35
PshBI ATTAAT 1 cut(s) 1074
Psp124BI GAGCTC 1 cut(s) 427
PspFI CCCAGC 2 cut(s) 1381, 1451
PspN4I GGNNCC 2 cut(s) 19, 803
PsrI GAACNNNNNNTAC 2 cut(s) 318, 350
PstI CTGCAG 2 cut(s) 286, 814
PstNI CAGNNNCTG 1 cut(s) 281
PvuII CAGCTG 3 cut(s) 287, 302, 1385
RsaI GTAC 3 cut(s) 19, 534, 1467
RsaNI GTAC 3 cut(s) 18, 533, 1466
RseI CAYNNNNRTG 2 cut(s) 224, 508
SacI GAGCTC 1 cut(s) 427
SaqAI TTAA 2 cut(s) 743, 1074
Sau3AI GATC 7 cut(s) 73, 84, 538, 557, 1130, 1292, 1552
SduI GDGCHC 4 cut(s) 427, 436, 885, 1306
SfaNI GCATC 1 cut(s) 1136
SfcI CTRYAG 3 cut(s) 33, 282, 810
SfuI TTCGAA 1 cut(s) 985
SmiMI CAYNNNNRTG 2 cut(s) 224, 508
SmlI CTYRAG 1 cut(s) 1441
SmoI CTYRAG 1 cut(s) 1441
SphI GCATGC 1 cut(s) 517
SseBI AGGCCT 1 cut(s) 952
SsiI CCGC 3 cut(s) 273, 290, 320
SspMI CTAG 3 cut(s) 345, 830, 1520
SstI GAGCTC 1 cut(s) 427
StuI AGGCCT 1 cut(s) 952
StyI CCWWGG 1 cut(s) 1519
TaaI ACNGT 4 cut(s) 199, 372, 644, 928
TaiI ACGT 2 cut(s) 433, 751
TaqI TCGA 5 cut(s) 626, 985, 992, 1291, 1322
TatI WGTACW 2 cut(s) 532, 1465
TauI GCSGC 3 cut(s) 275, 293, 323
TfiI GAWTC 3 cut(s) 229, 623, 1271
Tru1I TTAA 2 cut(s) 743, 1074
Tru9I TTAA 2 cut(s) 743, 1074
TscAI CASTG 7 cut(s) 204, 286, 441, 510, 1273, 1398, 1555
TseFI GTSAC 2 cut(s) 199, 647
Tsp45I GTSAC 2 cut(s) 199, 647
TspDTI ATGAA 6 cut(s) 123, 567, 626, 714, 834, 1494
TspRI CASTG 7 cut(s) 204, 286, 441, 510, 1273, 1398, 1555
VneI GTGCAC 2 cut(s) 432, 1302
VspI ATTAAT 1 cut(s) 1074
XagI CCTNNNNNAGG 1 cut(s) 1154
XapI RAATTY 2 cut(s) 987, 1495
XceI RCATGY 1 cut(s) 517
XmaJI CCTAGG 1 cut(s) 1519
XspI CTAG 3 cut(s) 345, 830, 1520
Zsp2I ATGCAT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.