FvH4_7g17520

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001398
Physical Location & Seq
Reverse (-)
14849287 .. 14852177
2891 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g17520.t1

Sequence Viewer

Length: 1590 bp
ATGGGGAAGACGGGAGGCTCCTCTTGGTTGACTGCTGTGAAAAGGGCTTTCAGATCTCCTACTAAGGACGATGAAGATCATAAGAAAAGAGAGAAGCGTCGGTGGATTTTTAGGAAGCCAACGAGTTTAAGTCAAGAAAGTTCAAAGGAGAAAACAGAGAATAATGTATCAGTTGCTACTGCAGAGCAGAGGCATGCTCTTGATGTTGCTGTGGCCACAGCCGAGGCAGCAATGGCGACGGCCCAAGCGGCCGCCGTCGAAGTGGCTCGGCTGACCAGGCCATCTAACCATGCCAGAGAGCAACAACTTCAGTTTGCAGCCATTGTCATCCAGACAGCTTTTAGAGGGTACTTGGCAAGGAGGGCGCTTCGTGCGCTTAAAGGGCTTGTGAAGCTGCAAGCTTTGGTGAGAGGACACAATGTGAGGAAGCAAGCGAAGATGACTCTTAGGTGTATGCAAGCTCTGGTTCGAGTTCAAGCTCGGGTTCTTGATCAGAGGACTAGGCTTTCCCACGAAGGGAGTAGAAAATCGGCTTTTAGTGACACTAATAGCAATAGCGCTTGGGAATCGCGGTATCATCAAGACATTTCAGACCGAAAATCCATTTCTAGAGAGGGAAGTAGTATAGCAGATGATTGGGACGAAAGGCCTCACACTATTGAGGAAGTAAAAGCAATGTTGCAGCATAGGAAAGAAGCTGCAATGAAGCGTGAGAAAGCTTTGTCCCACGCCTTCTCTCAACAGATATGGAGAAATGGGAGGAGTCCATCAATGGGGAATGAGGATGAGCTTGAAGAGAAACCCAAATGGCTAGACAGATGGATGGCCCCAAAGCCATGGGAAAGCAGCAGAGCAAGGGCTTCCACTGACCATAGAGATCCCATCAAAACTGTTGAAATTGACACCGCGCAGCCTTATTCAAGTTTATCATCCAATTTCCGAAGATCGAATGCCAACCAATACCACCAACAAAACCAACAGCAAAGACCTAGTTCGCCGCTCCATAGAGATAGATCCCACCAAAACCAACACTTCCACCACTCCCCCATCACACCCTCCCCATCAAAAAGCCGCCCTCCTATCCAAGTTCGCTCTGCGAGCCCTCGATGCGTTAGAGAAGATCGAAGCTCCAATGCGTCTCAAACCCCTAGCCTGAGGTCCAATTATTACTACAATGGCGGTGTTGTGCACCAACATAGCAGGGGCAGCACTAGCACTAGTGGTGGCAACGTTGGTGCCACATTGCCTAATTACATGGCGGCAACTGAATCTGCCAAGGCTCGTATTCGCTCCCAAAGTGCCCCTAGGCAAAGGCCTTCGACACCTGACAGGGACCGAGGAGGAGGAGTTGGATCGGTGAAGAAAAGGCTTTCGTTTCCAGCTCCTGATCCTTATGGTGCTGTTGGAATGGGAGCTTATGGAGGAGGAGGTTATGTGCATAGCTTAAGGAGTCCAAGCTTTAAGAGTGTGGTGAGTGGATCACACTTTGGGTTGGAGCAACAATCTAATTATTCTTCATGTTGTACTGAGAGTCATGGTGGTGAGGTATCTCCTTCTTCTACTAGTGACCTTAGAAGGTGGTTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

530

Amino Acids

59.12

Weight (kDa)

10.41

Isoelectric Point (pI)

66.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 367 - 469 2.7e-30 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1235
AccBSI CCGCTC 1 cut(s) 1000
AccII CGCG 2 cut(s) 571, 908
AciI CCGC 8 cut(s) 248, 252, 571, 906, 998, 1072, 1179, 1259
AclI AACGTT 1 cut(s) 1230
AclWI GGATC 5 cut(s) 872, 1008, 1360, 1382, 1486
AcoI YGGCCR 2 cut(s) 213, 249
AcuI CTGAAG 1 cut(s) 293
AdeI CACNNNGTG 1 cut(s) 421
AfaI GTAC 2 cut(s) 350, 1525
AfeI AGCGCT 1 cut(s) 559
AfiI CCNNNNNNNGG 2 cut(s) 516, 773
AflII CTTAAG 1 cut(s) 1444
AgsI TTSAA 5 cut(s) 144, 476, 794, 896, 921
AhlI ACTAGT 2 cut(s) 1217, 1562
AjnI CCWGG 1 cut(s) 275
Alw21I GWGCWC 1 cut(s) 1191
Alw26I GTCTC 1 cut(s) 1143
Alw44I GTGCAC 1 cut(s) 1187
AlwI GGATC 5 cut(s) 872, 1008, 1360, 1382, 1486
AlwNI CAGNNNCTG 1 cut(s) 1385
Ama87I CYCGRG 1 cut(s) 480
Aor51HI AGCGCT 1 cut(s) 559
AoxI GGCC 7 cut(s) 213, 240, 249, 278, 647, 825, 1313
ApaLI GTGCAC 1 cut(s) 1187
ApeKI GCWGC 8 cut(s) 227, 317, 394, 682, 698, 846, 910, 1206
AspA2I CCTAGG 1 cut(s) 1304
AspLEI GCGC 4 cut(s) 367, 376, 560, 910
AspS9I GGNCC 4 cut(s) 241, 826, 1158, 1333
AsuHPI GGTGA 4 cut(s) 418, 1369, 1483, 1553
AvaI CYCGRG 1 cut(s) 480
AvaII GGWCC 2 cut(s) 1158, 1333
AvrII CCTAGG 1 cut(s) 1304
AxyI CCTNAGG 1 cut(s) 1154
BaeGI GKGCMC 2 cut(s) 1191, 1303
BalI TGGCCA 1 cut(s) 215
BanI GGYRCC 1 cut(s) 1235
BanII GRGCYC 1 cut(s) 1103
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 1191
BbvI GCAGC 8 cut(s) 239, 329, 381, 685, 694, 858, 922, 1218
BccI CCATC 7 cut(s) 289, 775, 813, 817, 890, 1055, 1069
BceAI ACGGC 2 cut(s) 239, 255
BciT130I CCWGG 1 cut(s) 277
BclI TGATCA 1 cut(s) 490
BcoDI GTCTC 1 cut(s) 1143
BcuI ACTAGT 2 cut(s) 1217, 1562
BfaI CTAG 9 cut(s) 501, 609, 812, 990, 1149, 1212, 1218, 1305, 1563
BfmI CTRYAG 1 cut(s) 180
BfoI RGCGCY 2 cut(s) 368, 561
BfrI CTTAAG 1 cut(s) 1444
BglI GCCNNNNNGGC 1 cut(s) 248
BglII AGATCT 1 cut(s) 53
BlnI CCTAGG 1 cut(s) 1304
Bme1390I CCNGG 1 cut(s) 277
Bme18I GGWCC 2 cut(s) 1158, 1333
BmeT110I CYCGRG 1 cut(s) 480
BmgT120I GGNCC 4 cut(s) 241, 826, 1158, 1333
BmiI GGNNCC 4 cut(s) 19, 828, 1237, 1334
BmrFI CCNGG 1 cut(s) 277
BmsI GCATC 1 cut(s) 1097
BpiI GAAGAC 1 cut(s) 14
BplI GAGNNNNNCTC 2 cut(s) 181, 213
BsaBI GATNNNNATC 1 cut(s) 75
BsaJI CCNNGG 5 cut(s) 222, 836, 1275, 1304, 1336
BsaXI ACNNNNNCTCC 4 cut(s) 1332, 1362, 1419, 1449
Bsc4I CCNNNNNNNGG 2 cut(s) 516, 773
Bse21I CCTNAGG 1 cut(s) 1154
Bse3DI GCAATG 4 cut(s) 237, 681, 708, 1241
Bse8I GATNNNNATC 1 cut(s) 75
BseBI CCWGG 1 cut(s) 277
BseDI CCNNGG 5 cut(s) 222, 836, 1275, 1304, 1336
BseGI GGATG 4 cut(s) 327, 790, 828, 929
BseJI GATNNNNATC 1 cut(s) 75
BseLI CCNNNNNNNGG 2 cut(s) 516, 773
BseMI GCAATG 4 cut(s) 237, 681, 708, 1241
BseMII CTCAG 2 cut(s) 1145, 1518
BseRI GAGGAG 7 cut(s) 10, 775, 1353, 1356, 1359, 1437, 1440
BseSI GKGCMC 2 cut(s) 1191, 1303
BseX3I CGGCCG 1 cut(s) 249
BseXI GCAGC 8 cut(s) 239, 329, 381, 685, 694, 858, 922, 1218
Bsh1236I CGCG 2 cut(s) 571, 908
Bsh1285I CGRYCG 1 cut(s) 252
BshFI GGCC 7 cut(s) 215, 242, 251, 280, 649, 827, 1315
BshNI GGYRCC 1 cut(s) 1235
BsiEI CGRYCG 1 cut(s) 252
BsiHKAI GWGCWC 1 cut(s) 1191
BsiHKCI CYCGRG 1 cut(s) 480
BslFI GGGAC 3 cut(s) 653, 709, 1346
BslI CCNNNNNNNGG 2 cut(s) 516, 773
BsmAI GTCTC 1 cut(s) 1143
BsmBI CGTCTC 1 cut(s) 1143
BsmFI GGGAC 3 cut(s) 653, 709, 1346
BsmI GAATGC 1 cut(s) 955
BsnI GGCC 7 cut(s) 215, 242, 251, 280, 649, 827, 1315
BsoBI CYCGRG 1 cut(s) 480
Bsp1286I GDGCHC 3 cut(s) 1103, 1191, 1303
Bsp19I CCATGG 1 cut(s) 836
BspACI CCGC 8 cut(s) 248, 252, 571, 906, 998, 1072, 1179, 1259
BspANI GGCC 7 cut(s) 215, 242, 251, 280, 649, 827, 1315
BspCNI CTCAG 2 cut(s) 1146, 1519
BspFNI CGCG 2 cut(s) 571, 908
BspLI GGNNCC 4 cut(s) 19, 828, 1237, 1334
BspMAI CTGCAG 1 cut(s) 184
BspPI GGATC 5 cut(s) 872, 1008, 1360, 1382, 1486
BspT107I GGYRCC 1 cut(s) 1235
BspTI CTTAAG 1 cut(s) 1444
BsrBI CCGCTC 1 cut(s) 1000
BsrDI GCAATG 4 cut(s) 237, 681, 708, 1241
BssECI CCNNGG 5 cut(s) 222, 836, 1275, 1304, 1336
BssT1I CCWWGG 3 cut(s) 836, 1275, 1304
Bst2UI CCWGG 1 cut(s) 277
Bst4CI ACNGT 1 cut(s) 892
Bst6I CTCTTC 1 cut(s) 789
BstAFI CTTAAG 1 cut(s) 1444
BstC8I GCNNGC 5 cut(s) 195, 399, 432, 459, 1099
BstDEI CTNAG 5 cut(s) 63, 446, 1154, 1527, 1571
BstDSI CCRYGG 1 cut(s) 836
BstF5I GGATG 4 cut(s) 327, 790, 828, 929
BstFNI CGCG 2 cut(s) 571, 908
BstH2I RGCGCY 2 cut(s) 368, 561
BstHHI GCGC 4 cut(s) 367, 376, 560, 910
BstMAI GTCTC 1 cut(s) 1143
BstMCI CGRYCG 1 cut(s) 252
BstNI CCWGG 1 cut(s) 277
BstNSI RCATGY 1 cut(s) 197
BstSCI CCNGG 1 cut(s) 275
BstSFI CTRYAG 1 cut(s) 180
BstSLI GKGCMC 2 cut(s) 1191, 1303
BstUI CGCG 2 cut(s) 571, 908
BstV1I GCAGC 8 cut(s) 239, 329, 381, 685, 694, 858, 922, 1218
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 3 cut(s) 53, 877, 1013
BstXI CCANNNNNNTGG 1 cut(s) 837
BstYI RGATCY 3 cut(s) 53, 877, 1013
BstZI CGGCCG 1 cut(s) 249
Bsu36I CCTNAGG 1 cut(s) 1154
BsuRI GGCC 7 cut(s) 215, 242, 251, 280, 649, 827, 1315
BtgI CCRYGG 1 cut(s) 836
BtsCI GGATG 4 cut(s) 327, 790, 828, 929
BtsIMutI CAGTG 1 cut(s) 864
Cac8I GCNNGC 5 cut(s) 195, 399, 432, 459, 1099
CaiI CAGNNNCTG 1 cut(s) 1385
CciNI GCGGCCGC 1 cut(s) 249
CfoI GCGC 4 cut(s) 367, 376, 560, 910
Cfr13I GGNCC 4 cut(s) 241, 826, 1158, 1333
CseI GACGC 2 cut(s) 86, 1125
Csp6I GTAC 2 cut(s) 349, 1524
CviAII CATG 6 cut(s) 194, 290, 837, 1255, 1518, 1535
CviQI GTAC 2 cut(s) 349, 1524
DdeI CTNAG 5 cut(s) 63, 446, 1154, 1527, 1571
DraIII CACNNNGTG 1 cut(s) 421
EaeI YGGCCR 2 cut(s) 213, 249
EagI CGGCCG 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 789
EarI CTCTTC 1 cut(s) 789
EclXI CGGCCG 1 cut(s) 249
Eco130I CCWWGG 3 cut(s) 836, 1275, 1304
Eco147I AGGCCT 2 cut(s) 649, 1315
Eco24I GRGCYC 1 cut(s) 1103
Eco47I GGWCC 2 cut(s) 1158, 1333
Eco47III AGCGCT 1 cut(s) 559
Eco52I CGGCCG 1 cut(s) 249
Eco57I CTGAAG 1 cut(s) 293
Eco81I CCTNAGG 1 cut(s) 1154
Eco88I CYCGRG 1 cut(s) 480
EcoRII CCWGG 1 cut(s) 275
EcoT14I CCWWGG 3 cut(s) 836, 1275, 1304
EcoT38I GRGCYC 1 cut(s) 1103
ErhI CCWWGG 3 cut(s) 836, 1275, 1304
Esp3I CGTCTC 1 cut(s) 1143
FaeI CATG 6 cut(s) 197, 293, 840, 1258, 1521, 1538
FaqI GGGAC 3 cut(s) 653, 709, 1346
FatI CATG 6 cut(s) 193, 289, 836, 1254, 1517, 1534
FbaI TGATCA 1 cut(s) 490
FokI GGATG 4 cut(s) 314, 797, 835, 916
FriOI GRGCYC 1 cut(s) 1103
FspBI CTAG 9 cut(s) 501, 609, 812, 990, 1149, 1212, 1218, 1305, 1563
GlaI GCGC 4 cut(s) 366, 375, 559, 909
HaeII RGCGCY 2 cut(s) 368, 561
HaeIII GGCC 7 cut(s) 215, 242, 251, 280, 649, 827, 1315
HgaI GACGC 2 cut(s) 86, 1125
HhaI GCGC 4 cut(s) 367, 376, 560, 910
Hin1II CATG 6 cut(s) 197, 293, 840, 1258, 1521, 1538
Hin6I GCGC 4 cut(s) 365, 374, 558, 908
HinP1I GCGC 4 cut(s) 365, 374, 558, 908
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HindIII AAGCTT 3 cut(s) 399, 717, 1456
HinfI GANTC 6 cut(s) 442, 566, 763, 1268, 1450, 1531
HphI GGTGA 4 cut(s) 418, 1369, 1483, 1553
Hpy166II GTNNAC 2 cut(s) 30, 1189
Hpy188I TCNGA 4 cut(s) 53, 495, 592, 941
Hpy188III TCNNGA 7 cut(s) 134, 200, 331, 488, 581, 609, 1385
Hpy8I GTNNAC 2 cut(s) 30, 1189
Hpy99I CGWCG 3 cut(s) 102, 241, 260
HpyAV CCTTC 5 cut(s) 509, 742, 1326, 1563, 1569
HpyCH4III ACNGT 1 cut(s) 892
HpyCH4IV ACGT 1 cut(s) 1230
HpyCH4V TGCA 8 cut(s) 182, 317, 397, 457, 682, 701, 1189, 1438
HpyF3I CTNAG 5 cut(s) 63, 446, 1154, 1527, 1571
HpySE526I ACGT 1 cut(s) 1230
Hsp92II CATG 6 cut(s) 197, 293, 840, 1258, 1521, 1538
HspAI GCGC 4 cut(s) 365, 374, 558, 908
Ksp22I TGATCA 1 cut(s) 490
LmnI GCTCC 7 cut(s) 23, 1005, 1133, 1295, 1387, 1412, 1495
Lsp1109I GCAGC 8 cut(s) 239, 329, 381, 685, 694, 858, 922, 1218
LweI GCATC 1 cut(s) 1097
MaeI CTAG 9 cut(s) 501, 609, 812, 990, 1149, 1212, 1218, 1305, 1563
MaeII ACGT 1 cut(s) 1230
MaeIII GTNAC 2 cut(s) 539, 1565
MbiI CCGCTC 1 cut(s) 1000
MboII GAAGA 9 cut(s) 19, 86, 448, 806, 954, 1130, 1372, 1506, 1548
MflI RGATCY 3 cut(s) 53, 877, 1013
MhlI GDGCHC 3 cut(s) 1103, 1191, 1303
MlsI TGGCCA 1 cut(s) 215
MluCI AATT 5 cut(s) 897, 934, 1162, 1249, 1507
MluNI TGGCCA 1 cut(s) 215
MlyI GAGTC 4 cut(s) 436, 772, 1459, 1540
MmeI TCCRAC 3 cut(s) 1330, 1384, 1473
Mox20I TGGCCA 1 cut(s) 215
MscI TGGCCA 1 cut(s) 215
MseI TTAA 4 cut(s) 128, 378, 1445, 1461
MslI CAYNNNNRTG 1 cut(s) 1539
Msp20I TGGCCA 1 cut(s) 215
MspCI CTTAAG 1 cut(s) 1444
MspR9I CCNGG 1 cut(s) 277
Mva1269I GAATGC 1 cut(s) 955
MvaI CCWGG 1 cut(s) 277
MvnI CGCG 2 cut(s) 571, 908
NcoI CCATGG 1 cut(s) 836
NlaIII CATG 6 cut(s) 197, 293, 840, 1258, 1521, 1538
NlaIV GGNNCC 4 cut(s) 19, 828, 1237, 1334
NmeAIII GCCGAG 2 cut(s) 247, 247
NmuCI GTSAC 2 cut(s) 539, 1565
NotI GCGGCCGC 1 cut(s) 249
NspI RCATGY 1 cut(s) 197
PaeI GCATGC 1 cut(s) 197
PceI AGGCCT 2 cut(s) 649, 1315
PctI GAATGC 1 cut(s) 955
PfeI GAWTC 2 cut(s) 566, 1268
PleI GAGTC 4 cut(s) 436, 771, 1458, 1539
PpsI GAGTC 4 cut(s) 436, 771, 1458, 1539
Psp1406I AACGTT 1 cut(s) 1230
Psp6I CCWGG 1 cut(s) 275
PspGI CCWGG 1 cut(s) 275
PspN4I GGNNCC 4 cut(s) 19, 828, 1237, 1334
PspPI GGNCC 4 cut(s) 241, 826, 1158, 1333
PstI CTGCAG 1 cut(s) 184
PstNI CAGNNNCTG 1 cut(s) 1385
PsuI RGATCY 3 cut(s) 53, 877, 1013
RsaI GTAC 2 cut(s) 350, 1525
RsaNI GTAC 2 cut(s) 349, 1524
RseI CAYNNNNRTG 1 cut(s) 1539
SaqAI TTAA 4 cut(s) 128, 378, 1445, 1461
Sau96I GGNCC 4 cut(s) 241, 826, 1158, 1333
SchI GAGTC 4 cut(s) 436, 772, 1459, 1540
ScrFI CCNGG 1 cut(s) 277
SduI GDGCHC 3 cut(s) 1103, 1191, 1303
SfaNI GCATC 1 cut(s) 1097
SfcI CTRYAG 1 cut(s) 180
SfiI GGCCNNNNNGGCC 1 cut(s) 248
SinI GGWCC 2 cut(s) 1158, 1333
SmiMI CAYNNNNRTG 1 cut(s) 1539
SmlI CTYRAG 1 cut(s) 1444
SmoI CTYRAG 1 cut(s) 1444
SpeI ACTAGT 2 cut(s) 1217, 1562
SphI GCATGC 1 cut(s) 197
Sse9I AATT 5 cut(s) 897, 934, 1162, 1249, 1507
SseBI AGGCCT 2 cut(s) 649, 1315
SsiI CCGC 8 cut(s) 248, 252, 571, 906, 998, 1072, 1179, 1259
SspMI CTAG 9 cut(s) 501, 609, 812, 990, 1149, 1212, 1218, 1305, 1563
StuI AGGCCT 2 cut(s) 649, 1315
StyD4I CCNGG 1 cut(s) 275
StyI CCWWGG 3 cut(s) 836, 1275, 1304
TaaI ACNGT 1 cut(s) 892
TaiI ACGT 1 cut(s) 1233
TaqI TCGA 6 cut(s) 258, 469, 947, 1105, 1123, 1319
TaqII GACCGA 2 cut(s) 609, 1350
TasI AATT 5 cut(s) 897, 934, 1162, 1249, 1507
TatI WGTACW 1 cut(s) 1523
TauI GCSGC 5 cut(s) 251, 254, 1000, 1074, 1262
TfiI GAWTC 2 cut(s) 566, 1268
Tru1I TTAA 4 cut(s) 128, 378, 1445, 1461
Tru9I TTAA 4 cut(s) 128, 378, 1445, 1461
TscAI CASTG 1 cut(s) 871
TseFI GTSAC 2 cut(s) 539, 1565
TseI GCWGC 8 cut(s) 227, 317, 394, 682, 698, 846, 910, 1206
Tsp45I GTSAC 2 cut(s) 539, 1565
TspDTI ATGAA 3 cut(s) 87, 719, 1506
TspRI CASTG 1 cut(s) 871
Vha464I CTTAAG 1 cut(s) 1444
VneI GTGCAC 1 cut(s) 1187
VpaK11BI GGWCC 2 cut(s) 1158, 1333
XbaI TCTAGA 1 cut(s) 608
XceI RCATGY 1 cut(s) 197
XmaJI CCTAGG 1 cut(s) 1304
XspI CTAG 9 cut(s) 501, 609, 812, 990, 1149, 1212, 1218, 1305, 1563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.