Rh1DG449700

IQ-DOMAIN 14-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001398
Physical Location & Seq
Reverse (-)
65811320 .. 65814163
2844 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG449700.1

Sequence Viewer

Length: 1281 bp
ATGGCCGTGGCGACGACAGCAACTGCTCAGGCAGCGGTTGCAACTGTCCAAGCAGCGGTGGAAGCTGTTCGTCTCAGTACTAATTCTAGTTCTAGTAACTGTAATCTCTATAGGCCACGGCCTTCTATTTTCGTTAGAGATTATGCTGCCATTGCAATTCAGACTGCCTTCAGAGGATATCTGGCGAGAAGAGCTCTTCGGGCGTTGAAGGCGCTGGTGAAGCTTCAAGCATTAGTGAGAGGCCACAATGTTAGAAAGCGAGCAAAGACGACTCTACAATGCATGCAAGCACTGGTTCGAGTCCAAGCTCGGGTTCTTGACCAACGAACTAAGAGGCTTTCGCATCAAGGAAGCCTAGATTCCATGTTCAGTACAGATCATCCCAATTCTAGCTTATGGGGGTCACATTTCTCAGACAGGAAGTCCATTTCAAGAGAAGAAGAAAGTAGTACCTTGGATGATGATGAGGATGATTGGGTCCATTGGGATGATCTCCAGCCAAAAACACTAGAGCATATTCAAGCCATGTTGCAGAAAGCGAAAAAAGCTGCAGCATCAAATCGCGAAAGGACTACTCTTGCTTATGCCTTGTCCAACACAAACCAGGAGGAGCTAGAGGAGAAGTTTAATATGCTTGATGGGTGGACAAGAAGAAAGCAGTGGGAGAATGGGAGAGCTTCTTGTGATCAGCAAAGAGCAGACCCTATTGTAAAAACAGTTGAAATGGACACCTCTAGACCTTACTCTTATAGTCCAACCCCCAATTTGAAACGAGCAGAATATCAATACCAGAGCCATCACTACCAACAACAAGAAAGACCTCATCAGTACTCATATCCGGTTGCCTCTCCACAAGCACCAACCACACCATTTGCATCCCTTACAAGAATTCCGCAAGTGCACTCGGCTAGCCCTCGTTGCTACATTAGAGAAACCGGTCATGGAGTTGGAATGGGGGAGAGTGAAGTGCCAAACTACATGGCTGCAACAGCTTCAGCCAAGGCTAGGATTCGGTCACAGAGCGCACCAAGGCACAGACCTGCAACACAGGACAGAGAAAAACAGTGCTCAGCAAGGAAGCGCCTTTCATTCCCGGTTCCATCTGAGCCATCTGGTACTAATTGCACTTCAGACATCGAGTTGAAGAGCCCGAATTATAAGGGGATTAATGGAGGCCAAGCAATCCGAAGAGAGCACAAAGCAAGCAATGTGTCTTATTATGTAACAGACAGTGAGACATCTCCAATTTCCAGAGGTGAACTAAGAAGGTGGTTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

426

Amino Acids

48.08

Weight (kDa)

9.74

Isoelectric Point (pI)

57.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 269 - 377 9.2e-19 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1158
Acc36I ACCTGC 1 cut(s) 1048
AccII CGCG 1 cut(s) 564
AciI CCGC 3 cut(s) 35, 56, 893
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 888
AcuI CTGAAG 3 cut(s) 154, 978, 1113
AfaI GTAC 5 cut(s) 79, 373, 451, 830, 1117
AfiI CCNNNNNNNGG 3 cut(s) 55, 310, 1005
AgeI ACCGGT 1 cut(s) 935
AgsI TTSAA 7 cut(s) 208, 227, 432, 521, 722, 769, 1144
AhdI GACNNNNNGTC 1 cut(s) 421
AjnI CCWGG 1 cut(s) 603
AjuI GAANNNNNNNTTGG 2 cut(s) 297, 329
AluBI AGCT 9 cut(s) 65, 194, 223, 308, 393, 548, 613, 677, 992
AluI AGCT 9 cut(s) 65, 194, 223, 308, 393, 548, 613, 677, 992
Alw21I GWGCWC 4 cut(s) 196, 903, 1070, 1197
Alw26I GTCTC 2 cut(s) 77, 1229
Alw44I GTGCAC 1 cut(s) 899
AlwNI CAGNNNCTG 1 cut(s) 23
Ama87I CYCGRG 1 cut(s) 309
AoxI GGCC 5 cut(s) 3, 113, 119, 241, 1174
ApaLI GTGCAC 1 cut(s) 899
ApeKI GCWGC 6 cut(s) 32, 53, 146, 548, 551, 983
ApoI RAATTY 1 cut(s) 888
AseI ATTAAT 1 cut(s) 1167
AsiGI ACCGGT 1 cut(s) 935
Asp700I GAANNNNTTC 1 cut(s) 66
AspLEI GCGC 3 cut(s) 214, 1025, 1083
AspS9I GGNCC 1 cut(s) 478
AsuC2I CCSGG 1 cut(s) 1094
AsuHPI GGTGA 2 cut(s) 229, 1268
AsuNHI GCTAGC 1 cut(s) 908
AvaI CYCGRG 1 cut(s) 309
AvaII GGWCC 1 cut(s) 478
BaeGI GKGCMC 1 cut(s) 903
BanII GRGCYC 2 cut(s) 196, 1151
Bbv12I GWGCWC 4 cut(s) 196, 903, 1070, 1197
BbvI GCAGC 6 cut(s) 44, 65, 133, 535, 563, 970
BccI CCATC 4 cut(s) 632, 804, 1108, 1117
BceAI ACGGC 1 cut(s) 134
BciT130I CCWGG 1 cut(s) 605
BclI TGATCA 1 cut(s) 685
BcnI CCSGG 1 cut(s) 1094
BcoDI GTCTC 2 cut(s) 77, 1229
BfaI CTAG 9 cut(s) 87, 93, 356, 390, 509, 614, 735, 909, 1005
BfmI CTRYAG 2 cut(s) 109, 549
BfoI RGCGCY 2 cut(s) 215, 1084
BfuAI ACCTGC 1 cut(s) 1048
BisI GCNGC 6 cut(s) 33, 54, 147, 549, 552, 984
BlpI GCTNAGC 1 cut(s) 1069
BlsI GCNGC 6 cut(s) 34, 55, 148, 550, 553, 985
BmcAI AGTACT 2 cut(s) 79, 830
Bme1390I CCNGG 2 cut(s) 605, 1094
Bme18I GGWCC 1 cut(s) 478
BmeRI GACNNNNNGTC 1 cut(s) 421
BmeT110I CYCGRG 1 cut(s) 309
BmgT120I GGNCC 1 cut(s) 478
BmiI GGNNCC 2 cut(s) 479, 1098
BmrFI CCNGG 2 cut(s) 605, 1094
BmsI GCATC 3 cut(s) 352, 563, 884
BmtI GCTAGC 1 cut(s) 912
BplI GAGNNNNNCTC 2 cut(s) 178, 210
BpmI CTGGAG 1 cut(s) 479
Bpu10I CCTNAGC 1 cut(s) 27
Bpu1102I GCTNAGC 1 cut(s) 1069
BpuMI CCSGG 1 cut(s) 1094
BsaJI CCNNGG 5 cut(s) 6, 116, 453, 999, 1028
BsaWI WCCGGW 2 cut(s) 838, 935
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 310, 1005
Bse118I RCCGGY 1 cut(s) 935
Bse1I ACTGG 1 cut(s) 297
Bse3DI GCAATG 2 cut(s) 150, 1213
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 5 cut(s) 6, 116, 453, 999, 1028
BseGI GGATG 5 cut(s) 379, 463, 475, 493, 875
BseLI CCNNNNNNNGG 3 cut(s) 55, 310, 1005
BseMI GCAATG 2 cut(s) 150, 1213
BseMII CTCAG 5 cut(s) 41, 88, 426, 1083, 1095
BseNI ACTGG 1 cut(s) 297
BseRI GAGGAG 2 cut(s) 623, 632
BseSI GKGCMC 1 cut(s) 903
BseXI GCAGC 6 cut(s) 44, 65, 133, 535, 563, 970
Bsh1236I CGCG 1 cut(s) 564
BshFI GGCC 5 cut(s) 5, 115, 121, 243, 1176
BshTI ACCGGT 1 cut(s) 935
BsiHKAI GWGCWC 4 cut(s) 196, 903, 1070, 1197
BsiHKCI CYCGRG 1 cut(s) 309
BsiSI CCGG 3 cut(s) 839, 936, 1094
BslI CCNNNNNNNGG 3 cut(s) 55, 310, 1005
BsmAI GTCTC 2 cut(s) 77, 1229
BsmBI CGTCTC 1 cut(s) 77
BsnI GGCC 5 cut(s) 5, 115, 121, 243, 1176
BsoBI CYCGRG 1 cut(s) 309
Bsp1286I GDGCHC 5 cut(s) 196, 903, 1070, 1151, 1197
Bsp143I GATC 3 cut(s) 376, 490, 685
Bsp1720I GCTNAGC 1 cut(s) 1069
Bsp68I TCGCGA 1 cut(s) 564
BspACI CCGC 3 cut(s) 35, 56, 893
BspANI GGCC 5 cut(s) 5, 115, 121, 243, 1176
BspCNI CTCAG 5 cut(s) 40, 87, 425, 1082, 1096
BspFNI CGCG 1 cut(s) 564
BspLI GGNNCC 2 cut(s) 479, 1098
BspMAI CTGCAG 1 cut(s) 553
BspMI ACCTGC 1 cut(s) 1048
BspOI GCTAGC 1 cut(s) 912
BspQI GCTCTTC 3 cut(s) 184, 201, 1139
BsrDI GCAATG 2 cut(s) 150, 1213
BsrFI RCCGGY 1 cut(s) 935
BsrI ACTGG 1 cut(s) 297
BssAI RCCGGY 1 cut(s) 935
BssECI CCNNGG 5 cut(s) 6, 116, 453, 999, 1028
BssMI GATC 3 cut(s) 376, 490, 685
BssT1I CCWWGG 3 cut(s) 453, 999, 1028
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 5 cut(s) 46, 101, 718, 1065, 1232
Bst6I CTCTTC 4 cut(s) 184, 201, 1139, 1183
BstAPI GCANNNNNTGC 1 cut(s) 38
BstC8I GCNNGC 5 cut(s) 261, 284, 288, 910, 1204
BstDEI CTNAG 7 cut(s) 27, 74, 330, 412, 1069, 1104, 1262
BstDSI CCRYGG 2 cut(s) 6, 116
BstF5I GGATG 5 cut(s) 379, 463, 475, 493, 875
BstFNI CGCG 1 cut(s) 564
BstH2I RGCGCY 2 cut(s) 215, 1084
BstHHI GCGC 3 cut(s) 214, 1025, 1083
BstKTI GATC 3 cut(s) 379, 493, 688
BstMAI GTCTC 2 cut(s) 77, 1229
BstMBI GATC 3 cut(s) 376, 490, 685
BstNI CCWGG 1 cut(s) 605
BstNSI RCATGY 1 cut(s) 286
BstSCI CCNGG 2 cut(s) 603, 1092
BstSFI CTRYAG 2 cut(s) 109, 549
BstSLI GKGCMC 1 cut(s) 903
BstUI CGCG 1 cut(s) 564
BstV1I GCAGC 6 cut(s) 44, 65, 133, 535, 563, 970
BsuRI GGCC 5 cut(s) 5, 115, 121, 243, 1176
BtgI CCRYGG 2 cut(s) 6, 116
BtsCI GGATG 5 cut(s) 379, 463, 475, 493, 875
BtsI GCAGTG 1 cut(s) 665
BtsIMutI CAGTG 4 cut(s) 290, 665, 1070, 1237
BtuMI TCGCGA 1 cut(s) 564
BveI ACCTGC 1 cut(s) 1048
Cac8I GCNNGC 5 cut(s) 261, 284, 288, 910, 1204
CaiI CAGNNNCTG 1 cut(s) 23
CfoI GCGC 3 cut(s) 214, 1025, 1083
Cfr10I RCCGGY 1 cut(s) 935
Cfr13I GGNCC 1 cut(s) 478
Csp6I GTAC 5 cut(s) 78, 372, 450, 829, 1116
CspAI ACCGGT 1 cut(s) 935
CspCI CAANNNNNGTGG 2 cut(s) 853, 888
CviAII CATG 5 cut(s) 283, 364, 526, 941, 979
CviQI GTAC 5 cut(s) 78, 372, 450, 829, 1116
DdeI CTNAG 7 cut(s) 27, 74, 330, 412, 1069, 1104, 1262
DpnI GATC 3 cut(s) 378, 492, 687
DpnII GATC 3 cut(s) 376, 490, 685
DriI GACNNNNNGTC 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 184, 201, 1139, 1183
Eam1105I GACNNNNNGTC 1 cut(s) 421
EarI CTCTTC 4 cut(s) 184, 201, 1139, 1183
Ecl136II GAGCTC 1 cut(s) 194
Eco130I CCWWGG 3 cut(s) 453, 999, 1028
Eco24I GRGCYC 2 cut(s) 196, 1151
Eco32I GATATC 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 478
Eco53kI GAGCTC 1 cut(s) 194
Eco57I CTGAAG 3 cut(s) 154, 978, 1113
Eco88I CYCGRG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 194
EcoRI GAATTC 1 cut(s) 888
EcoRII CCWGG 1 cut(s) 603
EcoRV GATATC 1 cut(s) 179
EcoT14I CCWWGG 3 cut(s) 453, 999, 1028
EcoT22I ATGCAT 1 cut(s) 284
EcoT38I GRGCYC 2 cut(s) 196, 1151
ErhI CCWWGG 3 cut(s) 453, 999, 1028
Esp3I CGTCTC 1 cut(s) 77
FaeI CATG 5 cut(s) 286, 367, 529, 944, 982
FatI CATG 5 cut(s) 282, 363, 525, 940, 978
FbaI TGATCA 1 cut(s) 685
Fnu4HI GCNGC 6 cut(s) 33, 54, 147, 549, 552, 984
FokI GGATG 5 cut(s) 366, 470, 482, 500, 862
FriOI GRGCYC 2 cut(s) 196, 1151
Fsp4HI GCNGC 6 cut(s) 33, 54, 147, 549, 552, 984
FspBI CTAG 9 cut(s) 87, 93, 356, 390, 509, 614, 735, 909, 1005
GlaI GCGC 3 cut(s) 213, 1024, 1082
GluI GCNGC 6 cut(s) 33, 54, 147, 549, 552, 984
GsuI CTGGAG 1 cut(s) 479
HaeII RGCGCY 2 cut(s) 215, 1084
HaeIII GGCC 5 cut(s) 5, 115, 121, 243, 1176
HapII CCGG 3 cut(s) 839, 936, 1094
HhaI GCGC 3 cut(s) 214, 1025, 1083
Hin1II CATG 5 cut(s) 286, 367, 529, 944, 982
Hin6I GCGC 3 cut(s) 212, 1023, 1081
HinP1I GCGC 3 cut(s) 212, 1023, 1081
HindIII AAGCTT 1 cut(s) 221
HinfI GANTC 4 cut(s) 271, 300, 359, 1009
HpaII CCGG 3 cut(s) 839, 936, 1094
HphI GGTGA 2 cut(s) 229, 1268
Hpy166II GTNNAC 3 cut(s) 645, 901, 1259
Hpy188I TCNGA 6 cut(s) 162, 173, 415, 1105, 1132, 1187
Hpy188III TCNNGA 5 cut(s) 317, 432, 563, 735, 1251
Hpy8I GTNNAC 3 cut(s) 645, 901, 1259
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 4 cut(s) 132, 178, 202, 1260
HpyCH4III ACNGT 5 cut(s) 46, 101, 718, 1065, 1232
HpyF3I CTNAG 7 cut(s) 27, 74, 330, 412, 1069, 1104, 1262
Hsp92II CATG 5 cut(s) 286, 367, 529, 944, 982
HspAI GCGC 3 cut(s) 212, 1023, 1081
Ksp22I TGATCA 1 cut(s) 685
Kzo9I GATC 3 cut(s) 376, 490, 685
LguI GCTCTTC 3 cut(s) 184, 201, 1139
LmnI GCTCC 1 cut(s) 610
Lsp1109I GCAGC 6 cut(s) 44, 65, 133, 535, 563, 970
LweI GCATC 3 cut(s) 352, 563, 884
MaeI CTAG 9 cut(s) 87, 93, 356, 390, 509, 614, 735, 909, 1005
MaeIII GTNAC 4 cut(s) 95, 402, 1014, 1222
MalI GATC 3 cut(s) 378, 492, 687
MboI GATC 3 cut(s) 376, 490, 685
MboII GAAGA 7 cut(s) 188, 201, 449, 452, 663, 1156, 1200
MhlI GDGCHC 5 cut(s) 196, 903, 1070, 1151, 1197
MluCI AATT 8 cut(s) 82, 156, 385, 763, 888, 1120, 1153, 1245
MlyI GAGTC 2 cut(s) 265, 309
MmeI TCCRAC 3 cut(s) 618, 779, 928
Mph1103I ATGCAT 1 cut(s) 284
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 2 cut(s) 627, 1167
MslI CAYNNNNRTG 1 cut(s) 486
MspA1I CMGCKG 2 cut(s) 35, 56
MspI CCGG 3 cut(s) 839, 936, 1094
MspR9I CCNGG 2 cut(s) 605, 1094
MvaI CCWGG 1 cut(s) 605
MvnI CGCG 1 cut(s) 564
NciI CCSGG 1 cut(s) 1094
NdeII GATC 3 cut(s) 376, 490, 685
NheI GCTAGC 1 cut(s) 908
NlaIII CATG 5 cut(s) 286, 367, 529, 944, 982
NlaIV GGNNCC 2 cut(s) 479, 1098
NmeAIII GCCGAG 1 cut(s) 884
NmuCI GTSAC 2 cut(s) 402, 1014
NruI TCGCGA 1 cut(s) 564
NsiI ATGCAT 1 cut(s) 284
NspI RCATGY 1 cut(s) 286
PaeI GCATGC 1 cut(s) 286
PciSI GCTCTTC 3 cut(s) 184, 201, 1139
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 2 cut(s) 359, 1009
PinAI ACCGGT 1 cut(s) 935
PkrI GCNGC 6 cut(s) 34, 55, 148, 550, 553, 985
PleI GAGTC 2 cut(s) 265, 308
PpsI GAGTC 2 cut(s) 265, 308
PshBI ATTAAT 1 cut(s) 1167
PsiI TTATAA 1 cut(s) 1158
Psp124BI GAGCTC 1 cut(s) 196
Psp6I CCWGG 1 cut(s) 603
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 2 cut(s) 479, 1098
PspPI GGNCC 1 cut(s) 478
PstI CTGCAG 1 cut(s) 553
PstNI CAGNNNCTG 1 cut(s) 23
RruI TCGCGA 1 cut(s) 564
RsaI GTAC 5 cut(s) 79, 373, 451, 830, 1117
RsaNI GTAC 5 cut(s) 78, 372, 450, 829, 1116
RseI CAYNNNNRTG 1 cut(s) 486
SacI GAGCTC 1 cut(s) 196
SapI GCTCTTC 3 cut(s) 184, 201, 1139
SaqAI TTAA 2 cut(s) 627, 1167
SatI GCNGC 6 cut(s) 33, 54, 147, 549, 552, 984
Sau3AI GATC 3 cut(s) 376, 490, 685
Sau96I GGNCC 1 cut(s) 478
ScaI AGTACT 2 cut(s) 79, 830
SchI GAGTC 2 cut(s) 265, 309
ScrFI CCNGG 2 cut(s) 605, 1094
SduI GDGCHC 5 cut(s) 196, 903, 1070, 1151, 1197
SfaNI GCATC 3 cut(s) 352, 563, 884
SfcI CTRYAG 2 cut(s) 109, 549
SinI GGWCC 1 cut(s) 478
SmiMI CAYNNNNRTG 1 cut(s) 486
SphI GCATGC 1 cut(s) 286
Sse9I AATT 8 cut(s) 82, 156, 385, 763, 888, 1120, 1153, 1245
SsiI CCGC 3 cut(s) 35, 56, 893
SspMI CTAG 9 cut(s) 87, 93, 356, 390, 509, 614, 735, 909, 1005
SstI GAGCTC 1 cut(s) 196
StyD4I CCNGG 2 cut(s) 603, 1092
StyI CCWWGG 3 cut(s) 453, 999, 1028
TaaI ACNGT 5 cut(s) 46, 101, 718, 1065, 1232
TaqI TCGA 2 cut(s) 298, 1137
TaqII GACCGA 1 cut(s) 1002
TasI AATT 8 cut(s) 82, 156, 385, 763, 888, 1120, 1153, 1245
TatI WGTACW 3 cut(s) 77, 371, 828
TfiI GAWTC 2 cut(s) 359, 1009
Tru1I TTAA 2 cut(s) 627, 1167
Tru9I TTAA 2 cut(s) 627, 1167
TscAI CASTG 4 cut(s) 297, 665, 1070, 1237
TseFI GTSAC 2 cut(s) 402, 1014
TseI GCWGC 6 cut(s) 32, 53, 146, 548, 551, 983
Tsp45I GTSAC 2 cut(s) 402, 1014
TspDTI ATGAA 1 cut(s) 1077
TspRI CASTG 4 cut(s) 297, 665, 1070, 1237
VneI GTGCAC 1 cut(s) 899
VpaK11BI GGWCC 1 cut(s) 478
VspI ATTAAT 1 cut(s) 1167
XapI RAATTY 1 cut(s) 888
XbaI TCTAGA 1 cut(s) 734
XceI RCATGY 1 cut(s) 286
XmnI GAANNNNTTC 1 cut(s) 66
XspI CTAG 9 cut(s) 87, 93, 356, 390, 509, 614, 735, 909, 1005
ZrmI AGTACT 2 cut(s) 79, 830
Zsp2I ATGCAT 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.