FvH4_5g16380

alpha-N-acetylglucosaminidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000933
Physical Location & Seq
Reverse (-)
9317749 .. 9323472
5724 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16380.t2

Sequence Viewer

Length: 2649 bp
ATGGCTTCCGCCACTTCTCCCGCCATTTCTACCGTTACCATCTTCACACTCTTCTTCTTCTTCACCTTGTGCGTCCTCTCCTCCTCATCACCATCCACCATCGGTGTCGGCTACATTTCGAACGTTCTAGAACTTCAGGACCGCGAGAGGGCCCCGCCCTATGTCCAAGTCGCCGCCGCTCGCGGAGTCCTCCACCGCCTTCTTCCTTCCCACTCCTCCAGCTTCGACTTCCAAATCGTCTCGAAGGAGCAATGTGGTGGTGCTTCCTGTTTTATGATTAAGAACCATCCTTCTTTTCGTAGACGAGGAATCGATCCACAGATTCTGATTTCCGGAGTCACTGGAGTGGAGATTTTGGCTGGTCTGCACTGGTATTTGAAGAATTGGTGCGGCTCACATATATCTTGGGATAAAACTGGCGGTGCGCAACTATTTTCAGTCCCAAAAGCAGGTTTACTCTCTTGTGTAGGAGATGGTGGCATTTTAGTCCAGAGACCTGTTCCTTGGAACTATTACCAGAATGCTGTTACATCTAGCTATTCTTTGGCCTGGTGGGACTGGATTAGATGGGAAAAGGAGATTGATATGATGGCCCTCCAGGGTATCAATTTGCCATTAGCATTTACAGGTCAAGAGGCTATATGGCAGAAAGTTTTCCAGAAATTTAACATAAGCAAATCAGATTTGGATGATTTCTTTGGAGGCCCTGCATTCCTTTCATGGTCTCGAATGGGAAATCTGCACGGATGGGGTGGACCTCTCCCACAAAGTTGGCTTGATCAACAGCTGATTCTGCAGAAAAGAATTCTTGACAGAATGTATGAACTTGGAATGACTCCAGTTCTTCCAGCATTTTCCGGGAATGTTCCAGCAGCACTCAAAACTATATATCCAGCAGCAAAGATAACACAGTTAGGAAACTGGTTTTCTGTTAAGAGTGACCCTAGATGGACGTGCACTTACCTTCTTGATGCAACTGATCCCTTATTTGTTGAAATTGGGAAAACATTCATTGAGGAACAACTAAAAGAATACGGAAGGACAAGTCACATATACAACTGTGATACTTTCGATGAGAATACTCCACCCGTTGATGATCCAGAGTACATCTCTGCATTAGGTAAAACAATATTTAAGGGACTGCAAAGTGGAGATAAGGACGGGGTCTGGCTAATGCAGGGGTGGTTATTTTCATATGATCCATTCTGGAGGCCTGCACAAATGAAGGCACTTTTACATTCTGTTCCTGCAGGGAGGATGGTAGTCCTTGATCTGTTTGCTGAAGTGAAACCAATTTGGACTACTTCAGAGCAGTTCTATGGTGTTCCTTACATCTGGAAAGTCATTGCTCCTTTTCTTGATTGGGAATTAGCATTTTTTGGTATTCATTACAGGTGTATGCTGCACAACTTTGCAGGAAACGTTGAGATGTACGGCGTTTTGGATGCAATAGCATCCGGACCAATTGATGCTTGGACAAGTGAAAACTCAACCATGGTTGGGGTTGGAATGTCAATGGAAGGTATAGAACAGAATCCTGTTGTTTATGATCTAATGTCTGAGATGGCTTTTCAGCAGAACAAAGTTGATGTTAAGGACTGGATTAATCTGTACTCAACGAGACGTTATGGTCGTGCAGTTCCTTTAGTGCAAGACGCCTGGAGTATATTGCGTCATACCACCTATAATTGCACTGATGGTGCCTACAATTGTGTCTTCTCCATTCTCAATTTGAAGAGGGCCTTAAGGTCTTTGAACTCTTCAACTTTGCAAGATTGCACGGCTGGATCTTGTTCCAAAAAGCATGCATACACTGACAAAAATAGGGATGTGATTGTGGCATTTCCGGATGTTGATCCATCCTTTATAGCAAGACCGCCTCAAGGTTATTACCAAAATGAGAAATCATTAGTGTCAAGAAGAGCAGAACTCCTGGAGGAAGTAACTGATTCATTCGAGCGACCTCATTTATGGTATTCGACTTCTGAAGTAGTACATGCATTAGAACTTTTCATTGCAAGTGGAGATGAACTCTCAGGAAGCAACACTTACAGGTATGACCTTGTAGACCTGACAAGACAAGCTTTAGCCAAGTATGCAAATGAGCTGTTTCTGAAGGTCATAGAAGCCTATCATTTAAATGATACCCTTGAAGTAGTTGGCCTCAGCCAGAAGTTTTTGGAACTGGTTGAAGACATGGACACACTCTTAGCATGCCATGATGGATTTCTTCTAGGACCGTGGTTGGAAAGCGCAAAGAAACTTGCCCAAGACAAAGAGCAGGAAATACAGTTTGAATGGAATGCTAGAACTCAAATAACTATGTGGTTCGACAACACAGAAGAGGAGGCAAGTCTTCTTCGCGACTACGGAAACAAGTACTGGAGCGGGCTTCTGCGAGACTACTATGGTCCCCGAGCAGCCATATACTTCAAGTACCTGATAAAAAGCTTGGACGAGGGTAGTGATTTTGACTTGAAGAACTGGAGGAGGGAGTGGATCAAGCTCACAAATGACTGGCAAAGCAGCCGGAACACATTCCCGGTGAAAAGCACCGGAAATGCGGTGACTACTTCTCGGTTGCTTTTCGAAAAGTACTTACGAAGCGCTGACATAGAATCACCGGATGAAGCTGCTATTATGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
NAGLU NAGLU_C NAGLU_N

Protein Analysis

883

Amino Acids

100.22

Weight (kDa)

5.34

Isoelectric Point (pI)

46.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAGLU_N PF12971 58 - 145 2.2e-21 Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain
NAGLU PF05089 171 - 452 4.9e-116 Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
NAGLU PF05089 464 - 525 4.1e-18 Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
NAGLU_C PF12972 534 - 829 3.5e-86 Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1629
Acc16I TGCGCA 1 cut(s) 426
Acc36I ACCTGC 1 cut(s) 440
AccB1I GGYRCC 1 cut(s) 1700
AccBSI CCGCTC 2 cut(s) 179, 2388
AccI GTMKAC 2 cut(s) 301, 2067
AccII CGCG 3 cut(s) 144, 183, 2364
AccIII TCCGGA 3 cut(s) 332, 1457, 1846
AclI AACGTT 2 cut(s) 123, 1422
AclWI GGATC 7 cut(s) 308, 974, 1091, 1193, 1795, 1850, 2507
AcsI RAATTY 2 cut(s) 662, 804
AcuI CTGAAG 5 cut(s) 119, 1290, 1302, 2007, 2135
AcyI GRCGYC 1 cut(s) 1656
AdeI CACNNNGTG 1 cut(s) 69
AfaI GTAC 7 cut(s) 1106, 1433, 1613, 1995, 2381, 2438, 2597
AfeI AGCGCT 1 cut(s) 2608
AfiI CCNNNNNNNGG 4 cut(s) 148, 449, 1500, 1883
AflII CTTAAG 1 cut(s) 1744
AjiI CACGTC 1 cut(s) 954
AjnI CCWGG 4 cut(s) 548, 597, 1658, 1932
AleI CACNNNNGTG 1 cut(s) 344
AluBI AGCT 8 cut(s) 222, 537, 787, 2084, 2107, 2451, 2506, 2633
AluI AGCT 8 cut(s) 222, 537, 787, 2084, 2107, 2451, 2506, 2633
Alw21I GWGCWC 1 cut(s) 959
Alw26I GTCTC 5 cut(s) 244, 487, 729, 1615, 2394
Alw44I GTGCAC 1 cut(s) 955
AlwI GGATC 7 cut(s) 308, 974, 1091, 1193, 1795, 1850, 2507
AlwNI CAGNNNCTG 1 cut(s) 325
Ama87I CYCGRG 1 cut(s) 2415
Aor13HI TCCGGA 3 cut(s) 332, 1457, 1846
Aor51HI AGCGCT 1 cut(s) 2608
AoxI GGCC 7 cut(s) 150, 546, 591, 703, 1211, 1740, 2161
ApaI GGGCCC 1 cut(s) 154
ApaLI GTGCAC 1 cut(s) 955
ApeKI GCWGC 6 cut(s) 872, 896, 1402, 2420, 2526, 2633
ApoI RAATTY 2 cut(s) 662, 804
AseI ATTAAT 1 cut(s) 1605
Asp700I GAANNNNTTC 3 cut(s) 653, 1007, 2537
AspLEI GCGC 3 cut(s) 427, 2255, 2609
AsuC2I CCSGG 2 cut(s) 859, 2543
AsuHPI GGTGA 5 cut(s) 55, 81, 2557, 2578, 2613
AsuII TTCGAA 2 cut(s) 119, 2589
AvaI CYCGRG 1 cut(s) 2415
AvaII GGWCC 5 cut(s) 139, 755, 1460, 2237, 2411
BaeGI GKGCMC 2 cut(s) 154, 959
BanI GGYRCC 1 cut(s) 1700
BanII GRGCYC 1 cut(s) 154
BbsI GAAGAC 3 cut(s) 1708, 2199, 2348
Bbv12I GWGCWC 1 cut(s) 959
BbvCI CCTCAGC 1 cut(s) 2165
BbvI GCAGC 6 cut(s) 884, 908, 1389, 2432, 2538, 2620
BceAI ACGGC 2 cut(s) 1450, 1797
BciT130I CCWGG 4 cut(s) 550, 599, 1660, 1934
BclI TGATCA 1 cut(s) 778
BcnI CCSGG 2 cut(s) 859, 2543
BcoDI GTCTC 5 cut(s) 244, 487, 729, 1615, 2394
BfaI CTAG 5 cut(s) 128, 534, 945, 2234, 2307
BfmI CTRYAG 2 cut(s) 794, 1248
BfoI RGCGCY 1 cut(s) 2610
BfrI CTTAAG 1 cut(s) 1744
BfuAI ACCTGC 1 cut(s) 440
BisI GCNGC 9 cut(s) 174, 177, 391, 873, 897, 1403, 2421, 2527, 2634
BlsI GCNGC 9 cut(s) 175, 178, 392, 874, 898, 1404, 2422, 2528, 2635
BmcAI AGTACT 2 cut(s) 2381, 2597
Bme1390I CCNGG 6 cut(s) 550, 599, 859, 1660, 1934, 2543
Bme18I GGWCC 5 cut(s) 139, 755, 1460, 2237, 2411
BmeT110I CYCGRG 1 cut(s) 2415
BmgBI CACGTC 1 cut(s) 954
BmiI GGNNCC 4 cut(s) 152, 153, 1702, 2413
BmrFI CCNGG 6 cut(s) 550, 599, 859, 1660, 1934, 2543
BmsI GCATC 4 cut(s) 961, 1435, 1459, 1463
BpiI GAAGAC 3 cut(s) 1708, 2199, 2348
BplI GAGNNNNNCTC 6 cut(s) 1094, 1126, 1914, 1946, 2016, 2048
BpmI CTGGAG 9 cut(s) 202, 363, 581, 822, 1228, 1681, 1955, 2404, 2506
Bpu10I CCTNAGC 1 cut(s) 2165
Bpu14I TTCGAA 2 cut(s) 119, 2589
BpuEI CTTGAG 1 cut(s) 1866
BpuMI CCSGG 2 cut(s) 859, 2543
Bsa29I ATCGAT 1 cut(s) 312
BsaBI GATNNNNATC 1 cut(s) 1854
BsaHI GRCGYC 1 cut(s) 1656
BsaI GGTCTC 2 cut(s) 487, 729
BsaJI CCNNGG 4 cut(s) 503, 598, 1494, 2240
BsaWI WCCGGW 5 cut(s) 332, 1457, 1846, 2555, 2623
Bsc4I CCNNNNNNNGG 4 cut(s) 148, 449, 1500, 1883
Bse3DI GCAATG 3 cut(s) 257, 1344, 2013
Bse8I GATNNNNATC 1 cut(s) 1854
BseAI TCCGGA 3 cut(s) 332, 1457, 1846
BseBI CCWGG 4 cut(s) 550, 599, 1660, 1934
BseCI ATCGAT 1 cut(s) 312
BseDI CCNNGG 4 cut(s) 503, 598, 1494, 2240
BseJI GATNNNNATC 1 cut(s) 1854
BseLI CCNNNNNNNGG 4 cut(s) 148, 449, 1500, 1883
BseMI GCAATG 3 cut(s) 257, 1344, 2013
BseMII CTCAG 3 cut(s) 1551, 2049, 2179
BseRI GAGGAG 5 cut(s) 70, 73, 205, 2360, 2503
BseSI GKGCMC 2 cut(s) 154, 959
BseXI GCAGC 6 cut(s) 884, 908, 1389, 2432, 2538, 2620
BsgI GTGCAG 5 cut(s) 350, 725, 1200, 1388, 1656
Bsh1236I CGCG 3 cut(s) 144, 183, 2364
BshFI GGCC 7 cut(s) 152, 548, 593, 705, 1213, 1742, 2163
BshNI GGYRCC 1 cut(s) 1700
BshVI ATCGAT 1 cut(s) 312
BsiHKAI GWGCWC 1 cut(s) 959
BsiHKCI CYCGRG 1 cut(s) 2415
BsiSI CCGG 8 cut(s) 333, 858, 1458, 1847, 2530, 2543, 2556, 2624
BslFI GGGAC 4 cut(s) 425, 569, 1152, 2397
BslI CCNNNNNNNGG 4 cut(s) 148, 449, 1500, 1883
BsmAI GTCTC 5 cut(s) 244, 487, 729, 1615, 2394
BsmBI CGTCTC 2 cut(s) 244, 1615
BsmFI GGGAC 4 cut(s) 425, 569, 1152, 2397
BsmI GAATGC 3 cut(s) 526, 710, 2308
BsnI GGCC 7 cut(s) 152, 548, 593, 705, 1213, 1742, 2163
Bso31I GGTCTC 2 cut(s) 487, 729
BsoBI CYCGRG 1 cut(s) 2415
Bsp119I TTCGAA 2 cut(s) 119, 2589
Bsp120I GGGCCC 1 cut(s) 150
Bsp1286I GDGCHC 2 cut(s) 154, 959
Bsp13I TCCGGA 3 cut(s) 332, 1457, 1846
Bsp19I CCATGG 1 cut(s) 1494
Bsp68I TCGCGA 1 cut(s) 2364
BspANI GGCC 7 cut(s) 152, 548, 593, 705, 1213, 1742, 2163
BspCNI CTCAG 3 cut(s) 1552, 2048, 2178
BspDI ATCGAT 1 cut(s) 312
BspEI TCCGGA 3 cut(s) 332, 1457, 1846
BspFNI CGCG 3 cut(s) 144, 183, 2364
BspLI GGNNCC 4 cut(s) 152, 153, 1702, 2413
BspMAI CTGCAG 2 cut(s) 798, 1252
BspMI ACCTGC 1 cut(s) 440
BspPI GGATC 7 cut(s) 308, 974, 1091, 1193, 1795, 1850, 2507
BspQI GCTCTTC 1 cut(s) 1915
BspT104I TTCGAA 2 cut(s) 119, 2589
BspT107I GGYRCC 1 cut(s) 1700
BspTI CTTAAG 1 cut(s) 1744
BspTNI GGTCTC 2 cut(s) 487, 729
BsrBI CCGCTC 2 cut(s) 179, 2388
BsrDI GCAATG 3 cut(s) 257, 1344, 2013
BssECI CCNNGG 4 cut(s) 503, 598, 1494, 2240
BssNI GRCGYC 1 cut(s) 1656
BssT1I CCWWGG 2 cut(s) 503, 1494
Bst2UI CCWGG 4 cut(s) 550, 599, 1660, 1934
Bst4CI ACNGT 5 cut(s) 34, 912, 1061, 2241, 2292
Bst6I CTCTTC 5 cut(s) 56, 1730, 1765, 1915, 2337
BstACI GRCGYC 1 cut(s) 1656
BstAFI CTTAAG 1 cut(s) 1744
BstBI TTCGAA 2 cut(s) 119, 2589
BstC8I GCNNGC 5 cut(s) 181, 1215, 1806, 2215, 2390
BstDEI CTNAG 4 cut(s) 1560, 2035, 2165, 2209
BstDSI CCRYGG 2 cut(s) 1494, 2240
BstFNI CGCG 3 cut(s) 144, 183, 2364
BstH2I RGCGCY 1 cut(s) 2610
BstHHI GCGC 3 cut(s) 427, 2255, 2609
BstMAI GTCTC 5 cut(s) 244, 487, 729, 1615, 2394
BstMWI GCNNNNNNNGC 2 cut(s) 793, 2096
BstNI CCWGG 4 cut(s) 550, 599, 1660, 1934
BstNSI RCATGY 3 cut(s) 1808, 2000, 2217
BstSCI CCNGG 6 cut(s) 548, 597, 857, 1658, 1932, 2541
BstSFI CTRYAG 2 cut(s) 794, 1248
BstSLI GKGCMC 2 cut(s) 154, 959
BstUI CGCG 3 cut(s) 144, 183, 2364
BstV1I GCAGC 6 cut(s) 884, 908, 1389, 2432, 2538, 2620
BstV2I GAAGAC 3 cut(s) 1708, 2199, 2348
BstX2I RGATCY 1 cut(s) 1787
BstXI CCANNNNNNTGG 1 cut(s) 771
BstYI RGATCY 1 cut(s) 1787
Bsu15I ATCGAT 1 cut(s) 312
BsuRI GGCC 7 cut(s) 152, 548, 593, 705, 1213, 1742, 2163
BsuTUI ATCGAT 1 cut(s) 312
BtgI CCRYGG 2 cut(s) 1494, 2240
BtrI CACGTC 1 cut(s) 954
BtsIMutI CAGTG 4 cut(s) 339, 367, 1692, 1812
BtuMI TCGCGA 1 cut(s) 2364
BveI ACCTGC 1 cut(s) 440
Cac8I GCNNGC 5 cut(s) 181, 1215, 1806, 2215, 2390
CaiI CAGNNNCTG 1 cut(s) 325
CfoI GCGC 3 cut(s) 427, 2255, 2609
ClaI ATCGAT 1 cut(s) 312
CseI GACGC 3 cut(s) 61, 1661, 1664
Csp6I GTAC 7 cut(s) 1105, 1432, 1612, 1994, 2380, 2437, 2596
CviAII CATG 7 cut(s) 720, 1495, 1805, 1997, 2197, 2214, 2219
CviQI GTAC 7 cut(s) 1105, 1432, 1612, 1994, 2380, 2437, 2596
DdeI CTNAG 4 cut(s) 1560, 2035, 2165, 2209
DraI TTTAAA 1 cut(s) 2139
DraIII CACNNNGTG 1 cut(s) 69
DrdI GACNNNNNNGTC 1 cut(s) 1629
DseDI GACNNNNNNGTC 1 cut(s) 1629
Eam1104I CTCTTC 5 cut(s) 56, 1730, 1765, 1915, 2337
EarI CTCTTC 5 cut(s) 56, 1730, 1765, 1915, 2337
Eco130I CCWWGG 2 cut(s) 503, 1494
Eco147I AGGCCT 1 cut(s) 1213
Eco24I GRGCYC 1 cut(s) 154
Eco31I GGTCTC 2 cut(s) 487, 729
Eco47I GGWCC 5 cut(s) 139, 755, 1460, 2237, 2411
Eco47III AGCGCT 1 cut(s) 2608
Eco57I CTGAAG 5 cut(s) 119, 1290, 1302, 2007, 2135
Eco88I CYCGRG 1 cut(s) 2415
EcoO109I RGGNCCY 4 cut(s) 150, 151, 704, 1740
EcoRI GAATTC 1 cut(s) 804
EcoRII CCWGG 4 cut(s) 548, 597, 1658, 1932
EcoT14I CCWWGG 2 cut(s) 503, 1494
EcoT22I ATGCAT 2 cut(s) 1810, 2002
EcoT38I GRGCYC 1 cut(s) 154
ErhI CCWWGG 2 cut(s) 503, 1494
Esp3I CGTCTC 2 cut(s) 244, 1615
FaeI CATG 7 cut(s) 723, 1498, 1808, 2000, 2200, 2217, 2222
FalI AAGNNNNNCTT 6 cut(s) 1727, 1759, 2032, 2064, 2068, 2100
FaqI GGGAC 4 cut(s) 425, 569, 1152, 2397
FatI CATG 7 cut(s) 719, 1494, 1804, 1996, 2196, 2213, 2218
FauI CCCGC 3 cut(s) 28, 162, 2381
FauNDI CATATG 1 cut(s) 1195
FbaI TGATCA 1 cut(s) 778
FblI GTMKAC 2 cut(s) 301, 2067
Fnu4HI GCNGC 9 cut(s) 174, 177, 391, 873, 897, 1403, 2421, 2527, 2634
FriOI GRGCYC 1 cut(s) 154
Fsp4HI GCNGC 9 cut(s) 174, 177, 391, 873, 897, 1403, 2421, 2527, 2634
FspBI CTAG 5 cut(s) 128, 534, 945, 2234, 2307
FspI TGCGCA 1 cut(s) 426
GlaI GCGC 3 cut(s) 426, 2254, 2608
GluI GCNGC 9 cut(s) 174, 177, 391, 873, 897, 1403, 2421, 2527, 2634
GsuI CTGGAG 9 cut(s) 202, 363, 581, 822, 1228, 1681, 1955, 2404, 2506
HaeII RGCGCY 1 cut(s) 2610
HaeIII GGCC 7 cut(s) 152, 548, 593, 705, 1213, 1742, 2163
HapII CCGG 8 cut(s) 333, 858, 1458, 1847, 2530, 2543, 2556, 2624
HgaI GACGC 3 cut(s) 61, 1661, 1664
HhaI GCGC 3 cut(s) 427, 2255, 2609
Hin1I GRCGYC 1 cut(s) 1656
Hin1II CATG 7 cut(s) 723, 1498, 1808, 2000, 2200, 2217, 2222
Hin6I GCGC 3 cut(s) 425, 2253, 2607
HinP1I GCGC 3 cut(s) 425, 2253, 2607
HindIII AAGCTT 2 cut(s) 2082, 2449
HinfI GANTC 9 cut(s) 186, 309, 322, 336, 790, 835, 1534, 1949, 2618
HpaII CCGG 8 cut(s) 333, 858, 1458, 1847, 2530, 2543, 2556, 2624
HphI GGTGA 5 cut(s) 55, 81, 2557, 2578, 2613
Hpy166II GTNNAC 5 cut(s) 302, 455, 755, 957, 2068
Hpy188I TCNGA 6 cut(s) 327, 682, 1309, 1561, 1987, 2115
Hpy8I GTNNAC 5 cut(s) 302, 455, 755, 957, 2068
HpyAV CCTTC 9 cut(s) 209, 216, 238, 300, 974, 1032, 1219, 1514, 2110
HpyCH4III ACNGT 5 cut(s) 34, 912, 1061, 2241, 2292
HpyCH4IV ACGT 4 cut(s) 123, 953, 1422, 1624
HpyF10VI GCNNNNNNNGC 2 cut(s) 793, 2096
HpyF3I CTNAG 4 cut(s) 1560, 2035, 2165, 2209
HpySE526I ACGT 4 cut(s) 123, 953, 1422, 1624
Hsp92I GRCGYC 1 cut(s) 1656
Hsp92II CATG 7 cut(s) 723, 1498, 1808, 2000, 2200, 2217, 2222
HspAI GCGC 3 cut(s) 425, 2253, 2607
Kpn2I TCCGGA 3 cut(s) 332, 1457, 1846
Ksp22I TGATCA 1 cut(s) 778
LguI GCTCTTC 1 cut(s) 1915
LmnI GCTCC 3 cut(s) 247, 1354, 2385
Lsp1109I GCAGC 6 cut(s) 884, 908, 1389, 2432, 2538, 2620
LweI GCATC 4 cut(s) 961, 1435, 1459, 1463
MaeI CTAG 5 cut(s) 128, 534, 945, 2234, 2307
MaeII ACGT 4 cut(s) 123, 953, 1422, 1624
MaeIII GTNAC 7 cut(s) 34, 337, 526, 938, 1046, 1942, 2566
MbiI CCGCTC 2 cut(s) 179, 2388
MfeI CAATTG 2 cut(s) 1464, 1708
MflI RGATCY 1 cut(s) 1787
MhlI GDGCHC 2 cut(s) 154, 959
MlyI GAGTC 3 cut(s) 195, 345, 829
MmeI TCCRAC 2 cut(s) 1486, 2226
Mph1103I ATGCAT 2 cut(s) 1810, 2002
MroI TCCGGA 3 cut(s) 332, 1457, 1846
MroXI GAANNNNTTC 3 cut(s) 653, 1007, 2537
MseI TTAA 8 cut(s) 279, 666, 933, 1134, 1593, 1605, 1745, 2138
MslI CAYNNNNRTG 2 cut(s) 344, 2139
MspA1I CMGCKG 1 cut(s) 787
MspCI CTTAAG 1 cut(s) 1744
MspI CCGG 8 cut(s) 333, 858, 1458, 1847, 2530, 2543, 2556, 2624
MspR9I CCNGG 6 cut(s) 550, 599, 859, 1660, 1934, 2543
MunI CAATTG 2 cut(s) 1464, 1708
Mva1269I GAATGC 3 cut(s) 526, 710, 2308
MvaI CCWGG 4 cut(s) 550, 599, 1660, 1934
MvnI CGCG 3 cut(s) 144, 183, 2364
MwoI GCNNNNNNNGC 2 cut(s) 793, 2096
NciI CCSGG 2 cut(s) 859, 2543
NcoI CCATGG 1 cut(s) 1494
NdeI CATATG 1 cut(s) 1195
NlaIII CATG 7 cut(s) 723, 1498, 1808, 2000, 2200, 2217, 2222
NlaIV GGNNCC 4 cut(s) 152, 153, 1702, 2413
NmuCI GTSAC 4 cut(s) 337, 938, 1046, 2566
NruI TCGCGA 1 cut(s) 2364
NsbI TGCGCA 1 cut(s) 426
NsiI ATGCAT 2 cut(s) 1810, 2002
NspI RCATGY 3 cut(s) 1808, 2000, 2217
NspV TTCGAA 2 cut(s) 119, 2589
OliI CACNNNNGTG 1 cut(s) 344
PaeI GCATGC 2 cut(s) 1808, 2217
PceI AGGCCT 1 cut(s) 1213
PciSI GCTCTTC 1 cut(s) 1915
PcsI WCGNNNNNNNCGW 1 cut(s) 1630
PctI GAATGC 3 cut(s) 526, 710, 2308
PdmI GAANNNNTTC 3 cut(s) 653, 1007, 2537
PfeI GAWTC 6 cut(s) 309, 322, 790, 1534, 1949, 2618
PflFI GACNNNGTC 1 cut(s) 1163
PfoI TCCNGGA 2 cut(s) 857, 1932
PkrI GCNGC 9 cut(s) 175, 178, 392, 874, 898, 1404, 2422, 2528, 2635
PleI GAGTC 3 cut(s) 194, 344, 829
PpsI GAGTC 3 cut(s) 194, 344, 829
PshBI ATTAAT 1 cut(s) 1605
Psp1406I AACGTT 2 cut(s) 123, 1422
Psp6I CCWGG 4 cut(s) 548, 597, 1658, 1932
PspGI CCWGG 4 cut(s) 548, 597, 1658, 1932
PspN4I GGNNCC 4 cut(s) 152, 153, 1702, 2413
PspOMI GGGCCC 1 cut(s) 150
PstI CTGCAG 2 cut(s) 798, 1252
PstNI CAGNNNCTG 1 cut(s) 325
PsuI RGATCY 1 cut(s) 1787
PsyI GACNNNGTC 1 cut(s) 1163
PvuII CAGCTG 1 cut(s) 787
RruI TCGCGA 1 cut(s) 2364
RsaI GTAC 7 cut(s) 1106, 1433, 1613, 1995, 2381, 2438, 2597
RsaNI GTAC 7 cut(s) 1105, 1432, 1612, 1994, 2380, 2437, 2596
RseI CAYNNNNRTG 2 cut(s) 344, 2139
SapI GCTCTTC 1 cut(s) 1915
SaqAI TTAA 8 cut(s) 279, 666, 933, 1134, 1593, 1605, 1745, 2138
SatI GCNGC 9 cut(s) 174, 177, 391, 873, 897, 1403, 2421, 2527, 2634
SbfI CCTGCAGG 1 cut(s) 1252
ScaI AGTACT 2 cut(s) 2381, 2597
SchI GAGTC 3 cut(s) 195, 345, 829
ScrFI CCNGG 6 cut(s) 550, 599, 859, 1660, 1934, 2543
SdaI CCTGCAGG 1 cut(s) 1252
SduI GDGCHC 2 cut(s) 154, 959
SfaNI GCATC 4 cut(s) 961, 1435, 1459, 1463
SfcI CTRYAG 2 cut(s) 794, 1248
SfuI TTCGAA 2 cut(s) 119, 2589
SinI GGWCC 5 cut(s) 139, 755, 1460, 2237, 2411
SmiI ATTTAAAT 1 cut(s) 2139
SmiMI CAYNNNNRTG 2 cut(s) 344, 2139
SmlI CTYRAG 2 cut(s) 1744, 1881
SmoI CTYRAG 2 cut(s) 1744, 1881
SphI GCATGC 2 cut(s) 1808, 2217
Sse8387I CCTGCAGG 1 cut(s) 1252
SseBI AGGCCT 1 cut(s) 1213
SspI AATATT 1 cut(s) 1131
SspMI CTAG 5 cut(s) 128, 534, 945, 2234, 2307
StuI AGGCCT 1 cut(s) 1213
StyD4I CCNGG 6 cut(s) 548, 597, 857, 1658, 1932, 2541
StyI CCWWGG 2 cut(s) 503, 1494
SwaI ATTTAAAT 1 cut(s) 2139
TaaI ACNGT 5 cut(s) 34, 912, 1061, 2241, 2292
TaiI ACGT 4 cut(s) 126, 956, 1425, 1627
TatI WGTACW 5 cut(s) 1104, 1611, 1993, 2379, 2595
TauI GCSGC 3 cut(s) 176, 179, 393
TfiI GAWTC 6 cut(s) 309, 322, 790, 1534, 1949, 2618
Tru1I TTAA 8 cut(s) 279, 666, 933, 1134, 1593, 1605, 1745, 2138
Tru9I TTAA 8 cut(s) 279, 666, 933, 1134, 1593, 1605, 1745, 2138
TscAI CASTG 4 cut(s) 346, 374, 1699, 1819
TseFI GTSAC 4 cut(s) 337, 938, 1046, 2566
TseI GCWGC 6 cut(s) 872, 896, 1402, 2420, 2526, 2633
Tsp45I GTSAC 4 cut(s) 337, 938, 1046, 2566
TspGWI ACGGA 3 cut(s) 759, 1050, 2385
TspRI CASTG 4 cut(s) 346, 374, 1699, 1819
Tth111I GACNNNGTC 1 cut(s) 1163
Vha464I CTTAAG 1 cut(s) 1744
VneI GTGCAC 1 cut(s) 955
VpaK11BI GGWCC 5 cut(s) 139, 755, 1460, 2237, 2411
VspI ATTAAT 1 cut(s) 1605
XapI RAATTY 2 cut(s) 662, 804
XbaI TCTAGA 1 cut(s) 127
XceI RCATGY 3 cut(s) 1808, 2000, 2217
XcmI CCANNNNNNNNNTGG 1 cut(s) 1470
XmiI GTMKAC 2 cut(s) 301, 2067
XmnI GAANNNNTTC 3 cut(s) 653, 1007, 2537
XspI CTAG 5 cut(s) 128, 534, 945, 2234, 2307
ZrmI AGTACT 2 cut(s) 2381, 2597
Zsp2I ATGCAT 2 cut(s) 1810, 2002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.