RchiOBHm_Chr7g0179731

alpha-N-acetylglucosaminidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000933
Physical Location & Seq
Forward (+)
1926609 .. 1930107
3499 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16023

Sequence Viewer

Length: 1368 bp
ATGGCTCCCACCACATTTCCCGCCATTTCTACCATTACCATCTTCACGCTCTTCTTCTTCTTCACCTTGTGCGTCCTCTCCTCATCATCATCCACCATCGGAGTCGGCTACATATCGAGCGTTCTGGAACTTCAGGACCGCGAGAGGGCCTCTCCCTATGTCCAAGTCGCCGCCGCTCGCGGAGTACTCCGCCGCCTTCTTCCTTCCCAATCCTCCAGCTTCGACTTCCAAATCGTCTCTAAGGAGCAATGTGGTGGTGCTTCTTGCTTTATGGTTAAGAACCATCCTTCTTTTCGTAGACGAGGGGATCCACAGATTCTGATTTCCGGAGTCACTGGAGTGGAGATTTTGGCTGGTCTGCATTGGTATTTGAAGAATTGGTGTGGCTCACATATATCTTGGGATAAAACTGGTGGTGCGCAACTATTTTCAGTCCCAAAAGCAGGGTTTCTATCTCGTGTAGGAGATGGTGGCATTTTAGTCCAGAGACCTGTTCCTTGGAACTATTACCAAAATGCTGTCACATCTAGCTATTCTTTGGCTTGGTGGGACTGGAATAGATGGGAAAAGGAGATTGATATGATGGCCCTCCAGGGTATCAATTTGCCACTAGCATTTACAGGTCAAGAGGCTATATGGCAGAAAGTTTTCGAGAAATTTAATATGAGCAAATCAGATTTGGATGATTTCTTTGGAGGCCCTGCATTCCTTGCATGGTCTCGAATGGGAAATTTGCATGGATGGGGTGGACCTCTCCCGCAAAGTTGGCTTGATCAACAGCTGATTCTGCAGAAAAAAATTCTCAACAGAATGTATGAACTTGGAATGACTCCAGTCCTTCCTGCATTTTCTGGGAATGTTCCTGCAGCACTCAAAACTATATATCCATCAGCAAAGATTACACAGTTAGGAAACTGGTTTTCTGTTAAGAGTGACCCTAGATGGACCTGCACTTACCTTCTTGATGCAACTGATCCCTTATTTGTTGAAATTGGGAAAACATTCATTGAGGAACAACTAAAAGAATATGGAAGGACCAGTCACATTTACAACTGTGATACTTTTGATGAGAATACTCCACCTGTTGATGATCCGGAGTACATCTCTGCATTAGGTAAAACAATATTTAAGGGACTGAAAAGTGGAGATAAGGACGGTGTATGGCTAATGCAGGGATGGTTATTTTCATATGATCCATTCTGGAGGCCTGCACAGATGAAGGCACTCTTACATTCTGTTCCTGCAGGGAGGATGGTAGTCCTTGATCTCTTTGCTGAAGTGAAACCGATATGGACTACTTCAGAGCAGTTTTATGGTGTTCCTTACATCTGGAAAGTGTATGCTGCACAACTTTGCAGGAAACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
NAGLU NAGLU_C NAGLU_N

Protein Analysis

455

Amino Acids

51.4

Weight (kDa)

8.52

Isoelectric Point (pI)

48.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAGLU_N PF12971 57 - 142 2.4e-21 Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain
NAGLU PF05089 169 - 445 7.2e-117 Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 420
Acc36I ACCTGC 1 cut(s) 956
AccBSI CCGCTC 1 cut(s) 176
AccI GTMKAC 1 cut(s) 298
AccII CGCG 2 cut(s) 141, 180
AccIII TCCGGA 2 cut(s) 326, 1093
AciI CCGC 8 cut(s) 21, 139, 171, 174, 180, 190, 193, 758
AclI AACGTT 1 cut(s) 1363
AclWI GGATC 5 cut(s) 302, 315, 968, 1085, 1187
AcsI RAATTY 3 cut(s) 656, 730, 798
AcuI CTGAAG 3 cut(s) 116, 1284, 1296
AdeI CACNNNGTG 1 cut(s) 69
AfaI GTAC 2 cut(s) 186, 1100
AfiI CCNNNNNNNGG 2 cut(s) 145, 443
AgsI TTSAA 2 cut(s) 373, 989
AjnI CCWGG 1 cut(s) 591
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 3 cut(s) 219, 531, 781
AluI AGCT 3 cut(s) 219, 531, 781
Alw26I GTCTC 3 cut(s) 241, 481, 723
AlwI GGATC 5 cut(s) 302, 315, 968, 1085, 1187
AlwNI CAGNNNCTG 1 cut(s) 319
Aor13HI TCCGGA 2 cut(s) 326, 1093
AoxI GGCC 4 cut(s) 147, 585, 697, 1205
ApeKI GCWGC 2 cut(s) 866, 1343
ApoI RAATTY 3 cut(s) 656, 730, 798
Asp700I GAANNNNTTC 2 cut(s) 647, 1001
AspLEI GCGC 1 cut(s) 421
AspS9I GGNCC 7 cut(s) 136, 147, 586, 698, 749, 945, 1035
AsuHPI GGTGA 1 cut(s) 55
AvaII GGWCC 4 cut(s) 136, 749, 945, 1035
BamHI GGATCC 1 cut(s) 307
BauI CACGAG 1 cut(s) 456
BbvI GCAGC 2 cut(s) 878, 1330
BciT130I CCWGG 1 cut(s) 593
BclI TGATCA 1 cut(s) 772
BcoDI GTCTC 3 cut(s) 241, 481, 723
BfaI CTAG 3 cut(s) 528, 611, 939
BfmI CTRYAG 3 cut(s) 788, 864, 1242
BfuAI ACCTGC 1 cut(s) 956
BisI GCNGC 5 cut(s) 171, 174, 193, 867, 1344
BlsI GCNGC 5 cut(s) 172, 175, 194, 868, 1345
BmcAI AGTACT 1 cut(s) 186
Bme1390I CCNGG 1 cut(s) 593
Bme18I GGWCC 4 cut(s) 136, 749, 945, 1035
BmgT120I GGNCC 7 cut(s) 136, 147, 586, 698, 749, 945, 1035
BmiI GGNNCC 2 cut(s) 6, 309
BmrFI CCNGG 1 cut(s) 593
BmsI GCATC 1 cut(s) 955
BoxI GACNNNNGTC 1 cut(s) 833
BplI GAGNNNNNCTC 6 cut(s) 134, 166, 136, 168, 1088, 1120
BpmI CTGGAG 5 cut(s) 199, 357, 575, 816, 1222
BsaI GGTCTC 2 cut(s) 481, 723
BsaJI CCNNGG 2 cut(s) 497, 592
BsaWI WCCGGW 2 cut(s) 326, 1093
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 443
Bse1I ACTGG 6 cut(s) 340, 415, 557, 833, 920, 1038
Bse3DI GCAATG 1 cut(s) 254
BseAI TCCGGA 2 cut(s) 326, 1093
BseBI CCWGG 1 cut(s) 593
BseDI CCNNGG 2 cut(s) 497, 592
BseGI GGATG 6 cut(s) 89, 283, 688, 746, 1181, 1257
BseLI CCNNNNNNNGG 2 cut(s) 145, 443
BseMI GCAATG 1 cut(s) 254
BseNI ACTGG 6 cut(s) 340, 415, 557, 833, 920, 1038
BseRI GAGGAG 1 cut(s) 70
BseXI GCAGC 2 cut(s) 878, 1330
BsgI GTGCAG 3 cut(s) 934, 1194, 1329
Bsh1236I CGCG 2 cut(s) 141, 180
BshFI GGCC 4 cut(s) 149, 587, 699, 1207
BsiSI CCGG 2 cut(s) 327, 1094
BslFI GGGAC 3 cut(s) 419, 563, 1146
BslI CCNNNNNNNGG 2 cut(s) 145, 443
BsmAI GTCTC 3 cut(s) 241, 481, 723
BsmBI CGTCTC 1 cut(s) 241
BsmFI GGGAC 3 cut(s) 419, 563, 1146
BsmI GAATGC 1 cut(s) 704
BsnI GGCC 4 cut(s) 149, 587, 699, 1207
Bso31I GGTCTC 2 cut(s) 481, 723
Bsp13I TCCGGA 2 cut(s) 326, 1093
Bsp143I GATC 6 cut(s) 307, 772, 973, 1090, 1192, 1264
BspACI CCGC 8 cut(s) 21, 139, 171, 174, 180, 190, 193, 758
BspANI GGCC 4 cut(s) 149, 587, 699, 1207
BspEI TCCGGA 2 cut(s) 326, 1093
BspFNI CGCG 2 cut(s) 141, 180
BspLI GGNNCC 2 cut(s) 6, 309
BspMAI CTGCAG 3 cut(s) 792, 868, 1246
BspMI ACCTGC 1 cut(s) 956
BspPI GGATC 5 cut(s) 302, 315, 968, 1085, 1187
BspQI GCTCTTC 1 cut(s) 56
BspTNI GGTCTC 2 cut(s) 481, 723
BsrBI CCGCTC 1 cut(s) 176
BsrDI GCAATG 1 cut(s) 254
BsrI ACTGG 6 cut(s) 340, 415, 557, 833, 920, 1038
BssECI CCNNGG 2 cut(s) 497, 592
BssMI GATC 6 cut(s) 307, 772, 973, 1090, 1192, 1264
BssSI CACGAG 1 cut(s) 456
BssT1I CCWWGG 1 cut(s) 497
Bst2BI CACGAG 1 cut(s) 456
Bst2UI CCWGG 1 cut(s) 593
Bst4CI ACNGT 3 cut(s) 906, 1055, 1157
Bst6I CTCTTC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 710
BstC8I GCNNGC 2 cut(s) 178, 1209
BstDEI CTNAG 1 cut(s) 240
BstF5I GGATG 6 cut(s) 89, 283, 688, 746, 1181, 1257
BstFNI CGCG 2 cut(s) 141, 180
BstHHI GCGC 1 cut(s) 421
BstKTI GATC 6 cut(s) 310, 775, 976, 1093, 1195, 1267
BstMAI GTCTC 3 cut(s) 241, 481, 723
BstMBI GATC 6 cut(s) 307, 772, 973, 1090, 1192, 1264
BstMWI GCNNNNNNNGC 3 cut(s) 710, 766, 787
BstNI CCWGG 1 cut(s) 593
BstPAI GACNNNNGTC 1 cut(s) 833
BstSCI CCNGG 1 cut(s) 591
BstSFI CTRYAG 3 cut(s) 788, 864, 1242
BstUI CGCG 2 cut(s) 141, 180
BstV1I GCAGC 2 cut(s) 878, 1330
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BsuRI GGCC 4 cut(s) 149, 587, 699, 1207
BtsCI GGATG 6 cut(s) 89, 283, 688, 746, 1181, 1257
BtsIMutI CAGTG 1 cut(s) 333
BveI ACCTGC 1 cut(s) 956
Cac8I GCNNGC 2 cut(s) 178, 1209
CaiI CAGNNNCTG 1 cut(s) 319
CfoI GCGC 1 cut(s) 421
Cfr13I GGNCC 7 cut(s) 136, 147, 586, 698, 749, 945, 1035
CseI GACGC 1 cut(s) 61
Csp6I GTAC 2 cut(s) 185, 1099
CviAII CATG 2 cut(s) 714, 737
CviQI GTAC 2 cut(s) 185, 1099
DdeI CTNAG 1 cut(s) 240
DpnI GATC 6 cut(s) 309, 774, 975, 1092, 1194, 1266
DpnII GATC 6 cut(s) 307, 772, 973, 1090, 1192, 1264
DraIII CACNNNGTG 1 cut(s) 69
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
EciI GGCGGA 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 497
Eco147I AGGCCT 1 cut(s) 1207
Eco31I GGTCTC 2 cut(s) 481, 723
Eco47I GGWCC 4 cut(s) 136, 749, 945, 1035
Eco57I CTGAAG 3 cut(s) 116, 1284, 1296
EcoO109I RGGNCCY 2 cut(s) 147, 698
EcoRII CCWGG 1 cut(s) 591
EcoT14I CCWWGG 1 cut(s) 497
ErhI CCWWGG 1 cut(s) 497
Esp3I CGTCTC 1 cut(s) 241
FaeI CATG 2 cut(s) 717, 740
FalI AAGNNNNNCTT 2 cut(s) 1211, 1243
FaqI GGGAC 3 cut(s) 419, 563, 1146
FatI CATG 2 cut(s) 713, 736
FauI CCCGC 2 cut(s) 28, 765
FauNDI CATATG 1 cut(s) 1189
FbaI TGATCA 1 cut(s) 772
FblI GTMKAC 1 cut(s) 298
Fnu4HI GCNGC 5 cut(s) 171, 174, 193, 867, 1344
FokI GGATG 6 cut(s) 76, 270, 695, 753, 1188, 1264
Fsp4HI GCNGC 5 cut(s) 171, 174, 193, 867, 1344
FspBI CTAG 3 cut(s) 528, 611, 939
FspI TGCGCA 1 cut(s) 420
GlaI GCGC 1 cut(s) 420
GluI GCNGC 5 cut(s) 171, 174, 193, 867, 1344
GsuI CTGGAG 5 cut(s) 199, 357, 575, 816, 1222
HaeIII GGCC 4 cut(s) 149, 587, 699, 1207
HapII CCGG 2 cut(s) 327, 1094
HgaI GACGC 1 cut(s) 61
HhaI GCGC 1 cut(s) 421
Hin1II CATG 2 cut(s) 717, 740
Hin6I GCGC 1 cut(s) 419
HinP1I GCGC 1 cut(s) 419
HinfI GANTC 5 cut(s) 102, 316, 330, 784, 829
HpaII CCGG 2 cut(s) 327, 1094
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 2 cut(s) 299, 749
Hpy188I TCNGA 4 cut(s) 101, 321, 676, 1303
Hpy8I GTNNAC 2 cut(s) 299, 749
HpyAV CCTTC 7 cut(s) 206, 213, 297, 848, 968, 1026, 1213
HpyCH4III ACNGT 3 cut(s) 906, 1055, 1157
HpyCH4IV ACGT 1 cut(s) 1363
HpyF10VI GCNNNNNNNGC 3 cut(s) 710, 766, 787
HpyF3I CTNAG 1 cut(s) 240
HpySE526I ACGT 1 cut(s) 1363
Hsp92II CATG 2 cut(s) 717, 740
HspAI GCGC 1 cut(s) 419
Kpn2I TCCGGA 2 cut(s) 326, 1093
Ksp22I TGATCA 1 cut(s) 772
Kzo9I GATC 6 cut(s) 307, 772, 973, 1090, 1192, 1264
LguI GCTCTTC 1 cut(s) 56
LmnI GCTCC 2 cut(s) 10, 244
Lsp1109I GCAGC 2 cut(s) 878, 1330
LweI GCATC 1 cut(s) 955
MaeI CTAG 3 cut(s) 528, 611, 939
MaeII ACGT 1 cut(s) 1363
MaeIII GTNAC 4 cut(s) 331, 520, 932, 1040
MalI GATC 6 cut(s) 309, 774, 975, 1092, 1194, 1266
MbiI CCGCTC 1 cut(s) 176
MboI GATC 6 cut(s) 307, 772, 973, 1090, 1192, 1264
MboII GAAGA 7 cut(s) 34, 43, 46, 49, 52, 191, 385
MflI RGATCY 1 cut(s) 307
MluCI AATT 6 cut(s) 376, 601, 656, 730, 798, 990
MlyI GAGTC 3 cut(s) 111, 339, 823
MroI TCCGGA 2 cut(s) 326, 1093
MroXI GAANNNNTTC 2 cut(s) 647, 1001
MseI TTAA 4 cut(s) 276, 660, 927, 1128
MslI CAYNNNNRTG 1 cut(s) 338
MspA1I CMGCKG 1 cut(s) 781
MspI CCGG 2 cut(s) 327, 1094
MspR9I CCNGG 1 cut(s) 593
Mva1269I GAATGC 1 cut(s) 704
MvaI CCWGG 1 cut(s) 593
MvnI CGCG 2 cut(s) 141, 180
MwoI GCNNNNNNNGC 3 cut(s) 710, 766, 787
NdeI CATATG 1 cut(s) 1189
NdeII GATC 6 cut(s) 307, 772, 973, 1090, 1192, 1264
NlaIII CATG 2 cut(s) 717, 740
NlaIV GGNNCC 2 cut(s) 6, 309
NmuCI GTSAC 4 cut(s) 331, 520, 932, 1040
NsbI TGCGCA 1 cut(s) 420
OliI CACNNNNGTG 1 cut(s) 338
PceI AGGCCT 1 cut(s) 1207
PciSI GCTCTTC 1 cut(s) 56
PctI GAATGC 1 cut(s) 704
PdmI GAANNNNTTC 2 cut(s) 647, 1001
PfeI GAWTC 2 cut(s) 316, 784
PkrI GCNGC 5 cut(s) 172, 175, 194, 868, 1345
PleI GAGTC 3 cut(s) 110, 338, 823
PpsI GAGTC 3 cut(s) 110, 338, 823
PshAI GACNNNNGTC 1 cut(s) 833
Psp1406I AACGTT 1 cut(s) 1363
Psp6I CCWGG 1 cut(s) 591
PspGI CCWGG 1 cut(s) 591
PspN4I GGNNCC 2 cut(s) 6, 309
PspPI GGNCC 7 cut(s) 136, 147, 586, 698, 749, 945, 1035
PstI CTGCAG 3 cut(s) 792, 868, 1246
PstNI CAGNNNCTG 1 cut(s) 319
PsuI RGATCY 1 cut(s) 307
PvuII CAGCTG 1 cut(s) 781
RsaI GTAC 2 cut(s) 186, 1100
RsaNI GTAC 2 cut(s) 185, 1099
RseI CAYNNNNRTG 1 cut(s) 338
SapI GCTCTTC 1 cut(s) 56
SaqAI TTAA 4 cut(s) 276, 660, 927, 1128
SatI GCNGC 5 cut(s) 171, 174, 193, 867, 1344
Sau3AI GATC 6 cut(s) 307, 772, 973, 1090, 1192, 1264
Sau96I GGNCC 7 cut(s) 136, 147, 586, 698, 749, 945, 1035
SbfI CCTGCAGG 1 cut(s) 1246
ScaI AGTACT 1 cut(s) 186
SchI GAGTC 3 cut(s) 111, 339, 823
ScrFI CCNGG 1 cut(s) 593
SdaI CCTGCAGG 1 cut(s) 1246
SfaNI GCATC 1 cut(s) 955
SfcI CTRYAG 3 cut(s) 788, 864, 1242
SinI GGWCC 4 cut(s) 136, 749, 945, 1035
SmiMI CAYNNNNRTG 1 cut(s) 338
Sse8387I CCTGCAGG 1 cut(s) 1246
Sse9I AATT 6 cut(s) 376, 601, 656, 730, 798, 990
SseBI AGGCCT 1 cut(s) 1207
SsiI CCGC 8 cut(s) 21, 139, 171, 174, 180, 190, 193, 758
SspI AATATT 1 cut(s) 1125
SspMI CTAG 3 cut(s) 528, 611, 939
StuI AGGCCT 1 cut(s) 1207
StyD4I CCNGG 1 cut(s) 591
StyI CCWWGG 1 cut(s) 497
TaaI ACNGT 3 cut(s) 906, 1055, 1157
TaiI ACGT 1 cut(s) 1366
TaqI TCGA 4 cut(s) 116, 222, 651, 721
TasI AATT 6 cut(s) 376, 601, 656, 730, 798, 990
TatI WGTACW 2 cut(s) 184, 1098
TauI GCSGC 3 cut(s) 173, 176, 195
TfiI GAWTC 2 cut(s) 316, 784
Tru1I TTAA 4 cut(s) 276, 660, 927, 1128
Tru9I TTAA 4 cut(s) 276, 660, 927, 1128
TscAI CASTG 1 cut(s) 340
TseFI GTSAC 4 cut(s) 331, 520, 932, 1040
TseI GCWGC 2 cut(s) 866, 1343
Tsp45I GTSAC 4 cut(s) 331, 520, 932, 1040
TspDTI ATGAA 4 cut(s) 831, 994, 1176, 1232
TspRI CASTG 1 cut(s) 340
VpaK11BI GGWCC 4 cut(s) 136, 749, 945, 1035
XapI RAATTY 3 cut(s) 656, 730, 798
XmiI GTMKAC 1 cut(s) 298
XmnI GAANNNNTTC 2 cut(s) 647, 1001
XspI CTAG 3 cut(s) 528, 611, 939
ZrmI AGTACT 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.