Rh7AG027200

alpha-N-acetylglucosaminidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000933
Physical Location & Seq
Forward (+)
1802991 .. 1806187
3197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG027200.1

Sequence Viewer

Length: 1284 bp
ATGGCTCCCACCACATTTCCCGCCACTTCTACCATTACCATCTTCACGCTCTTCTTCTTCTTCTTCTTCACCTTGTGTGTCCTCTCCTCATCATCATCCACCATCGGAGTCGGCTACATATCCAGCGTTCTGGAACTTCAGGACCGCGAGAGGGCCTCTCCCTATGTCCAAGTCGCCGCCGCTCGCGGAGTACTCCGCCGCCTTCTTCCTTCCCAATCCTCCAGCTTCGACTTCCAAATCGTCTCTAAGGAGCAATGTGGTGGTGCTTCTTGCTTTATGATTAAGAACCATCCTTCTTTTCGTAGACGAGGGGATCCACAGATTCTGATTTCCGGAGTCACTGGAGTGGAGATTTTGGCTGGTCTGCATTGGTATTTGAAGAATTGGTGTGGCTCACATATATCTTGGGATAAAACTGGTGGTGCGCAACTATTTTCAGTCCCAAAAGCAGGGTTTCTATCTCGTGTAGGAGATGGTGGCATTTTAGTCCAGAGACCTGTTCCTTGGAACTATTACCAGAATGCTGTCACATCTAGCTATTCTTTGGCTTGGTGGGACTGGAATAGATGGGAAAAGGAGATTGATATGATGGCCCTCCAGGGTATCAATTTGCCACTAGCATTTACAGGTCAAGAGGCTATATGGCAGAAAGTTTTCGAGAAATTTAATATGAGCAAATCAGATTTGGATGATTTCTTTGGAGGCCCTGCATTCCTTGCATGGTCTCGAATGGGAAATTTGCATGGATGGGGTGGACCTCTCCCACAAAGTTGGCTTGATCAACAGCTGATTCTGCAGAAAAAAATTCTCAACAGAATGTATGAACTTGGAATGACTCCAGTCCTTCCAGCATTTTCTGGGAATGTTCCTGCAGCACTCAAAACTATATATCCATCAGCAAAGATTACACAGTTAGGAAACTGGTTTTCTGTTAAGAGTGACCCTAGATGGACCTGCACTTACCTTCTTGATGCAACTGATCCCTTATTTGTTGAAATTGGGAAAACATTCATTGAGGAACAACTAAAAGGTTGGTCGCATGAATCATTTGCCCTCCTCCGAAATGGATGGTTATTTTCATATGATCCATTCTGGAGGCCTGCACAGATGAAGGCACTCTTACATTCTGTTCCTGCAGGGAGGATGGTAGTCCTTGATCTGTTTGCTGAAGTGAAACCGATATGGACTACTTCAGAGCAGTTTTATGGTGTTCCTTACATCTGGAAAGTCATTGCTCCTTTTTATGATTGGGAAATCCCACAGTGTGAATATTTAATTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
NAGLU NAGLU_C NAGLU_N

Protein Analysis

427

Amino Acids

48.47

Weight (kDa)

7.6

Isoelectric Point (pI)

52.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAGLU_N PF12971 59 - 144 2.3e-21 Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain
NAGLU PF05089 171 - 346 2.3e-76 Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
NAGLU PF05089 354 - 414 3.7e-15 Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 426
Acc36I ACCTGC 1 cut(s) 962
AccBSI CCGCTC 1 cut(s) 182
AccI GTMKAC 1 cut(s) 304
AccII CGCG 2 cut(s) 147, 186
AccIII TCCGGA 1 cut(s) 332
AciI CCGC 7 cut(s) 21, 145, 177, 180, 186, 196, 199
AclWI GGATC 4 cut(s) 308, 321, 974, 1079
AcsI RAATTY 3 cut(s) 662, 736, 804
AcuI CTGAAG 3 cut(s) 122, 1176, 1188
AdeI CACNNNGTG 2 cut(s) 75, 1265
AfaI GTAC 1 cut(s) 192
AfiI CCNNNNNNNGG 2 cut(s) 151, 449
AgsI TTSAA 2 cut(s) 379, 995
AjnI CCWGG 1 cut(s) 597
AleI CACNNNNGTG 1 cut(s) 344
AluBI AGCT 3 cut(s) 225, 537, 787
AluI AGCT 3 cut(s) 225, 537, 787
Alw26I GTCTC 3 cut(s) 247, 487, 729
AlwI GGATC 4 cut(s) 308, 321, 974, 1079
AlwNI CAGNNNCTG 1 cut(s) 325
Aor13HI TCCGGA 1 cut(s) 332
AoxI GGCC 4 cut(s) 153, 591, 703, 1097
ApeKI GCWGC 1 cut(s) 872
ApoI RAATTY 3 cut(s) 662, 736, 804
Asp700I GAANNNNTTC 2 cut(s) 653, 1007
AspLEI GCGC 1 cut(s) 427
AspS9I GGNCC 6 cut(s) 142, 153, 592, 704, 755, 951
AsuHPI GGTGA 1 cut(s) 61
AvaII GGWCC 3 cut(s) 142, 755, 951
BamHI GGATCC 1 cut(s) 313
BauI CACGAG 1 cut(s) 462
BbvI GCAGC 1 cut(s) 884
BciT130I CCWGG 1 cut(s) 599
BclI TGATCA 1 cut(s) 778
BcoDI GTCTC 3 cut(s) 247, 487, 729
BfaI CTAG 3 cut(s) 534, 617, 945
BfmI CTRYAG 3 cut(s) 794, 870, 1134
BfuAI ACCTGC 1 cut(s) 962
BisI GCNGC 4 cut(s) 177, 180, 199, 873
BlsI GCNGC 4 cut(s) 178, 181, 200, 874
BmcAI AGTACT 1 cut(s) 192
Bme1390I CCNGG 1 cut(s) 599
Bme18I GGWCC 3 cut(s) 142, 755, 951
BmgT120I GGNCC 6 cut(s) 142, 153, 592, 704, 755, 951
BmiI GGNNCC 2 cut(s) 6, 315
BmrFI CCNGG 1 cut(s) 599
BmsI GCATC 1 cut(s) 961
BoxI GACNNNNGTC 1 cut(s) 839
BplI GAGNNNNNCTC 4 cut(s) 140, 172, 142, 174
BpmI CTGGAG 5 cut(s) 205, 363, 581, 822, 1114
BsaI GGTCTC 2 cut(s) 487, 729
BsaJI CCNNGG 2 cut(s) 503, 598
BsaWI WCCGGW 1 cut(s) 332
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 449
Bse1I ACTGG 5 cut(s) 346, 421, 563, 839, 926
Bse3DI GCAATG 2 cut(s) 260, 1230
BseAI TCCGGA 1 cut(s) 332
BseBI CCWGG 1 cut(s) 599
BseDI CCNNGG 2 cut(s) 503, 598
BseGI GGATG 6 cut(s) 95, 289, 694, 752, 1073, 1149
BseLI CCNNNNNNNGG 2 cut(s) 151, 449
BseMI GCAATG 2 cut(s) 260, 1230
BseNI ACTGG 5 cut(s) 346, 421, 563, 839, 926
BseRI GAGGAG 2 cut(s) 76, 1046
BseXI GCAGC 1 cut(s) 884
BsgI GTGCAG 2 cut(s) 940, 1086
Bsh1236I CGCG 2 cut(s) 147, 186
BshFI GGCC 4 cut(s) 155, 593, 705, 1099
BsiSI CCGG 1 cut(s) 333
BslFI GGGAC 2 cut(s) 425, 569
BslI CCNNNNNNNGG 2 cut(s) 151, 449
BsmAI GTCTC 3 cut(s) 247, 487, 729
BsmBI CGTCTC 1 cut(s) 247
BsmFI GGGAC 2 cut(s) 425, 569
BsmI GAATGC 2 cut(s) 526, 710
BsnI GGCC 4 cut(s) 155, 593, 705, 1099
Bso31I GGTCTC 2 cut(s) 487, 729
Bsp13I TCCGGA 1 cut(s) 332
Bsp143I GATC 5 cut(s) 313, 778, 979, 1084, 1156
BspACI CCGC 7 cut(s) 21, 145, 177, 180, 186, 196, 199
BspANI GGCC 4 cut(s) 155, 593, 705, 1099
BspEI TCCGGA 1 cut(s) 332
BspFNI CGCG 2 cut(s) 147, 186
BspLI GGNNCC 2 cut(s) 6, 315
BspMAI CTGCAG 3 cut(s) 798, 874, 1138
BspMI ACCTGC 1 cut(s) 962
BspPI GGATC 4 cut(s) 308, 321, 974, 1079
BspQI GCTCTTC 1 cut(s) 56
BspTNI GGTCTC 2 cut(s) 487, 729
BsrBI CCGCTC 1 cut(s) 182
BsrDI GCAATG 2 cut(s) 260, 1230
BsrI ACTGG 5 cut(s) 346, 421, 563, 839, 926
BssECI CCNNGG 2 cut(s) 503, 598
BssMI GATC 5 cut(s) 313, 778, 979, 1084, 1156
BssSI CACGAG 1 cut(s) 462
BssT1I CCWWGG 1 cut(s) 503
Bst2BI CACGAG 1 cut(s) 462
Bst2UI CCWGG 1 cut(s) 599
Bst4CI ACNGT 2 cut(s) 912, 1263
Bst6I CTCTTC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 716
BstC8I GCNNGC 2 cut(s) 184, 1101
BstDEI CTNAG 1 cut(s) 246
BstF5I GGATG 6 cut(s) 95, 289, 694, 752, 1073, 1149
BstFNI CGCG 2 cut(s) 147, 186
BstHHI GCGC 1 cut(s) 427
BstKTI GATC 5 cut(s) 316, 781, 982, 1087, 1159
BstMAI GTCTC 3 cut(s) 247, 487, 729
BstMBI GATC 5 cut(s) 313, 778, 979, 1084, 1156
BstMWI GCNNNNNNNGC 2 cut(s) 716, 793
BstNI CCWGG 1 cut(s) 599
BstPAI GACNNNNGTC 1 cut(s) 839
BstSCI CCNGG 1 cut(s) 597
BstSFI CTRYAG 3 cut(s) 794, 870, 1134
BstUI CGCG 2 cut(s) 147, 186
BstV1I GCAGC 1 cut(s) 884
BstX2I RGATCY 1 cut(s) 313
BstXI CCANNNNNNTGG 2 cut(s) 130, 771
BstYI RGATCY 1 cut(s) 313
BsuRI GGCC 4 cut(s) 155, 593, 705, 1099
BtsCI GGATG 6 cut(s) 95, 289, 694, 752, 1073, 1149
BtsIMutI CAGTG 2 cut(s) 339, 1268
BveI ACCTGC 1 cut(s) 962
Cac8I GCNNGC 2 cut(s) 184, 1101
CaiI CAGNNNCTG 1 cut(s) 325
CfoI GCGC 1 cut(s) 427
Cfr13I GGNCC 6 cut(s) 142, 153, 592, 704, 755, 951
Csp6I GTAC 1 cut(s) 191
CviAII CATG 3 cut(s) 720, 743, 1040
CviQI GTAC 1 cut(s) 191
DdeI CTNAG 1 cut(s) 246
DpnI GATC 5 cut(s) 315, 780, 981, 1086, 1158
DpnII GATC 5 cut(s) 313, 778, 979, 1084, 1156
DraIII CACNNNGTG 2 cut(s) 75, 1265
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
EciI GGCGGA 1 cut(s) 185
Eco130I CCWWGG 1 cut(s) 503
Eco147I AGGCCT 1 cut(s) 1099
Eco31I GGTCTC 2 cut(s) 487, 729
Eco47I GGWCC 3 cut(s) 142, 755, 951
Eco57I CTGAAG 3 cut(s) 122, 1176, 1188
EcoO109I RGGNCCY 2 cut(s) 153, 704
EcoRII CCWGG 1 cut(s) 597
EcoT14I CCWWGG 1 cut(s) 503
ErhI CCWWGG 1 cut(s) 503
Esp3I CGTCTC 1 cut(s) 247
FaeI CATG 3 cut(s) 723, 746, 1043
FalI AAGNNNNNCTT 2 cut(s) 1103, 1135
FaqI GGGAC 2 cut(s) 425, 569
FatI CATG 3 cut(s) 719, 742, 1039
FauI CCCGC 1 cut(s) 28
FauNDI CATATG 1 cut(s) 1081
FbaI TGATCA 1 cut(s) 778
FblI GTMKAC 1 cut(s) 304
Fnu4HI GCNGC 4 cut(s) 177, 180, 199, 873
FokI GGATG 6 cut(s) 82, 276, 701, 759, 1080, 1156
Fsp4HI GCNGC 4 cut(s) 177, 180, 199, 873
FspBI CTAG 3 cut(s) 534, 617, 945
FspI TGCGCA 1 cut(s) 426
GlaI GCGC 1 cut(s) 426
GluI GCNGC 4 cut(s) 177, 180, 199, 873
GsuI CTGGAG 5 cut(s) 205, 363, 581, 822, 1114
HaeIII GGCC 4 cut(s) 155, 593, 705, 1099
HapII CCGG 1 cut(s) 333
HhaI GCGC 1 cut(s) 427
Hin1II CATG 3 cut(s) 723, 746, 1043
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HinfI GANTC 6 cut(s) 108, 322, 336, 790, 835, 1043
HpaII CCGG 1 cut(s) 333
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 2 cut(s) 305, 755
Hpy188I TCNGA 5 cut(s) 107, 327, 682, 1061, 1195
Hpy8I GTNNAC 2 cut(s) 305, 755
HpyAV CCTTC 6 cut(s) 212, 219, 303, 854, 974, 1105
HpyCH4III ACNGT 2 cut(s) 912, 1263
HpyF10VI GCNNNNNNNGC 2 cut(s) 716, 793
HpyF3I CTNAG 1 cut(s) 246
Hsp92II CATG 3 cut(s) 723, 746, 1043
HspAI GCGC 1 cut(s) 425
Kpn2I TCCGGA 1 cut(s) 332
Ksp22I TGATCA 1 cut(s) 778
Kzo9I GATC 5 cut(s) 313, 778, 979, 1084, 1156
LguI GCTCTTC 1 cut(s) 56
LmnI GCTCC 3 cut(s) 10, 250, 1240
Lsp1109I GCAGC 1 cut(s) 884
LweI GCATC 1 cut(s) 961
MaeI CTAG 3 cut(s) 534, 617, 945
MaeIII GTNAC 3 cut(s) 337, 526, 938
MalI GATC 5 cut(s) 315, 780, 981, 1086, 1158
MbiI CCGCTC 1 cut(s) 182
MboI GATC 5 cut(s) 313, 778, 979, 1084, 1156
MboII GAAGA 9 cut(s) 34, 43, 46, 49, 52, 55, 58, 197, 391
MflI RGATCY 1 cut(s) 313
MluCI AATT 7 cut(s) 382, 607, 662, 736, 804, 996, 1275
MlyI GAGTC 3 cut(s) 117, 345, 829
MroI TCCGGA 1 cut(s) 332
MroXI GAANNNNTTC 2 cut(s) 653, 1007
MseI TTAA 4 cut(s) 282, 666, 933, 1274
MslI CAYNNNNRTG 1 cut(s) 344
MspA1I CMGCKG 1 cut(s) 787
MspI CCGG 1 cut(s) 333
MspR9I CCNGG 1 cut(s) 599
Mva1269I GAATGC 2 cut(s) 526, 710
MvaI CCWGG 1 cut(s) 599
MvnI CGCG 2 cut(s) 147, 186
MwoI GCNNNNNNNGC 2 cut(s) 716, 793
NdeI CATATG 1 cut(s) 1081
NdeII GATC 5 cut(s) 313, 778, 979, 1084, 1156
NlaIII CATG 3 cut(s) 723, 746, 1043
NlaIV GGNNCC 2 cut(s) 6, 315
NmuCI GTSAC 3 cut(s) 337, 526, 938
NsbI TGCGCA 1 cut(s) 426
OliI CACNNNNGTG 1 cut(s) 344
PceI AGGCCT 1 cut(s) 1099
PciSI GCTCTTC 1 cut(s) 56
PctI GAATGC 2 cut(s) 526, 710
PdmI GAANNNNTTC 2 cut(s) 653, 1007
PfeI GAWTC 3 cut(s) 322, 790, 1043
PkrI GCNGC 4 cut(s) 178, 181, 200, 874
PleI GAGTC 3 cut(s) 116, 344, 829
PpsI GAGTC 3 cut(s) 116, 344, 829
PshAI GACNNNNGTC 1 cut(s) 839
Psp6I CCWGG 1 cut(s) 597
PspGI CCWGG 1 cut(s) 597
PspN4I GGNNCC 2 cut(s) 6, 315
PspPI GGNCC 6 cut(s) 142, 153, 592, 704, 755, 951
PstI CTGCAG 3 cut(s) 798, 874, 1138
PstNI CAGNNNCTG 1 cut(s) 325
PsuI RGATCY 1 cut(s) 313
PvuII CAGCTG 1 cut(s) 787
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
RseI CAYNNNNRTG 1 cut(s) 344
SapI GCTCTTC 1 cut(s) 56
SaqAI TTAA 4 cut(s) 282, 666, 933, 1274
SatI GCNGC 4 cut(s) 177, 180, 199, 873
Sau3AI GATC 5 cut(s) 313, 778, 979, 1084, 1156
Sau96I GGNCC 6 cut(s) 142, 153, 592, 704, 755, 951
SbfI CCTGCAGG 1 cut(s) 1138
ScaI AGTACT 1 cut(s) 192
SchI GAGTC 3 cut(s) 117, 345, 829
ScrFI CCNGG 1 cut(s) 599
SdaI CCTGCAGG 1 cut(s) 1138
SfaNI GCATC 1 cut(s) 961
SfcI CTRYAG 3 cut(s) 794, 870, 1134
SinI GGWCC 3 cut(s) 142, 755, 951
SmiMI CAYNNNNRTG 1 cut(s) 344
Sse8387I CCTGCAGG 1 cut(s) 1138
Sse9I AATT 7 cut(s) 382, 607, 662, 736, 804, 996, 1275
SseBI AGGCCT 1 cut(s) 1099
SsiI CCGC 7 cut(s) 21, 145, 177, 180, 186, 196, 199
SspI AATATT 1 cut(s) 1271
SspMI CTAG 3 cut(s) 534, 617, 945
StuI AGGCCT 1 cut(s) 1099
StyD4I CCNGG 1 cut(s) 597
StyI CCWWGG 1 cut(s) 503
TaaI ACNGT 2 cut(s) 912, 1263
TaqI TCGA 3 cut(s) 228, 657, 727
TasI AATT 7 cut(s) 382, 607, 662, 736, 804, 996, 1275
TatI WGTACW 1 cut(s) 190
TauI GCSGC 3 cut(s) 179, 182, 201
TfiI GAWTC 3 cut(s) 322, 790, 1043
Tru1I TTAA 4 cut(s) 282, 666, 933, 1274
Tru9I TTAA 4 cut(s) 282, 666, 933, 1274
TscAI CASTG 2 cut(s) 346, 1268
TseFI GTSAC 3 cut(s) 337, 526, 938
TseI GCWGC 1 cut(s) 872
Tsp45I GTSAC 3 cut(s) 337, 526, 938
TspDTI ATGAA 5 cut(s) 837, 1000, 1056, 1068, 1124
TspRI CASTG 2 cut(s) 346, 1268
VpaK11BI GGWCC 3 cut(s) 142, 755, 951
XapI RAATTY 3 cut(s) 662, 736, 804
XmiI GTMKAC 1 cut(s) 304
XmnI GAANNNNTTC 2 cut(s) 653, 1007
XspI CTAG 3 cut(s) 534, 617, 945
ZrmI AGTACT 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.