Rh7AG027300

alpha-N-acetylglucosaminidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000933
Physical Location & Seq
Forward (+)
1806322 .. 1808416
2095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG027300.1

Sequence Viewer

Length: 1140 bp
ATGCTGCACAACTTTGCAGGAAACGTTGAGATGTACGGCGTTATGGATGCAATAGCATCTGGACCAATTGATGCTTGGACAAGTGAAAACTCAACCATGGTTGGGGTTGGAATGTCAATGGAAGGTATAGAACAGAACCCTGTTGTTTATGATCTCATGTCTGAGATGGCTTTTCAACAGAACAAAGTTGATGTTAAGGACTGGATTAATCTGTATTCAACGAGACGTTATGGTCGTGCAGTTCCTTTAGTGCAACGTGCCTGGAGTATATTACATCATACCATCTATAATTGCACTGATGGTGCCTACGACAAAAATAGGGATGTGATTGTGGCATTTCCGGACGTTGATCCATCCTTTATAGCAAGACCGTCTCAAGGTTATTACCAAAATGATAAATCATTAGTATCAAGAAGATCAGTCCTGAAGGAAATAACTGATTCATTTGAGCGACCTCATTTATGGTACTCGACTTCTGAAGTAGTACATGCATTAGAACTTTTTATTGCAAGTGGAGATGAACTCTCAGAAAGCAACACTTACAGGTATGACCTTGTTGACCTGACCAGACAAGCTTTAGCTAAGTATGCAAACCAGCTGTTTCTGAAGGTCGTAGAAGCCTATCACTTAAATGATACCCTTGAAGTAGTTGGCCTCAGCCAGAAGTTTTTGGAACTGGTTGAAGATATGGACACACTTTTAGCATGCCATGATGGATTTCTTCTTGGACCATGGTTGGAAAGCGCAAAGAAACTCGCCCAAGACAAAGAGCAGCAAATACAGTTTGAATGGAATGCTAGAACTCAAATAACTATGTGGTTTGACAACACAGAAGAGGAAGCAAGTCTTCTTCGCGACTATGGAAACAAGTATTGGAGTGGGCTTCTGCGAGACTACTATGGTCCCCGAGCAGCCATATACTTCAAGTATCTGATAAAAAGCTTGGACGAGGGTTATGATTTTGACTTGAAGAACTGGAGGAGGGAGTGGATCAAGCTCACCAATGACTGGCAAAACAGCAGGAACACATTCCCGGTGAAAAGCACCGGAAATGCGGTGACTACTTCAAGGTTGCTTTTCGAAAAGTACTTACGAAGCGCTGACATAGAATCACTGGATGAAGCTGCTATTATGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
NAGLU NAGLU_C NAGLU_N

Protein Analysis

379

Amino Acids

43.86

Weight (kDa)

4.89

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAGLU PF05089 1 - 59 7.1e-17 Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
NAGLU_C PF12972 68 - 362 1.6e-87 Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 231
AccB1I GGYRCC 1 cut(s) 302
AccB7I CCANNNNNTGG 1 cut(s) 1008
AccII CGCG 1 cut(s) 855
AccIII TCCGGA 1 cut(s) 340
AciI CCGC 1 cut(s) 1055
AclI AACGTT 1 cut(s) 24
AclWI GGATC 2 cut(s) 344, 998
AcuI CTGAAG 3 cut(s) 446, 498, 626
AfaI GTAC 4 cut(s) 35, 467, 486, 1088
AfeI AGCGCT 1 cut(s) 1099
AfiI CCNNNNNNNGG 3 cut(s) 102, 377, 1008
AgsI TTSAA 8 cut(s) 176, 219, 644, 683, 788, 925, 970, 1068
AjnI CCWGG 1 cut(s) 260
AjuI GAANNNNNNNTTGG 2 cut(s) 856, 888
AluBI AGCT 6 cut(s) 575, 581, 598, 942, 997, 1124
AluI AGCT 6 cut(s) 575, 581, 598, 942, 997, 1124
Alw26I GTCTC 3 cut(s) 217, 378, 885
AlwI GGATC 2 cut(s) 344, 998
Ama87I CYCGRG 1 cut(s) 906
Aor13HI TCCGGA 1 cut(s) 340
Aor51HI AGCGCT 1 cut(s) 1099
AoxI GGCC 1 cut(s) 652
ApeKI GCWGC 4 cut(s) 4, 772, 911, 1124
AseI ATTAAT 1 cut(s) 207
Asp700I GAANNNNTTC 1 cut(s) 1028
AspLEI GCGC 2 cut(s) 746, 1100
AspS9I GGNCC 3 cut(s) 62, 728, 902
AsuC2I CCSGG 1 cut(s) 1034
AsuHPI GGTGA 3 cut(s) 991, 1048, 1069
AsuII TTCGAA 1 cut(s) 1080
AvaI CYCGRG 1 cut(s) 906
AvaII GGWCC 3 cut(s) 62, 728, 902
BanI GGYRCC 1 cut(s) 302
BbsI GAAGAC 1 cut(s) 839
BbvCI CCTCAGC 1 cut(s) 656
BbvI GCAGC 3 cut(s) 784, 923, 1111
BccI CCATC 5 cut(s) 160, 290, 293, 361, 707
BceAI ACGGC 1 cut(s) 52
BciT130I CCWGG 1 cut(s) 262
BcnI CCSGG 1 cut(s) 1034
BcoDI GTCTC 3 cut(s) 217, 378, 885
BfaI CTAG 1 cut(s) 798
BfoI RGCGCY 1 cut(s) 1101
BisI GCNGC 4 cut(s) 5, 773, 912, 1125
BlsI GCNGC 4 cut(s) 6, 774, 913, 1126
BmcAI AGTACT 1 cut(s) 1088
Bme1390I CCNGG 2 cut(s) 262, 1034
Bme18I GGWCC 3 cut(s) 62, 728, 902
BmeT110I CYCGRG 1 cut(s) 906
BmgT120I GGNCC 3 cut(s) 62, 728, 902
BmiI GGNNCC 2 cut(s) 304, 904
BmrFI CCNGG 2 cut(s) 262, 1034
BmsI GCATC 3 cut(s) 37, 61, 65
BpiI GAAGAC 1 cut(s) 839
BplI GAGNNNNNCTC 2 cut(s) 507, 539
BpmI CTGGAG 2 cut(s) 283, 997
Bpu10I CCTNAGC 1 cut(s) 656
Bpu14I TTCGAA 1 cut(s) 1080
BpuEI CTTGAG 1 cut(s) 360
BpuMI CCSGG 1 cut(s) 1034
BsaJI CCNNGG 2 cut(s) 96, 731
BsaWI WCCGGW 2 cut(s) 340, 1046
Bsc4I CCNNNNNNNGG 3 cut(s) 102, 377, 1008
Bse1I ACTGG 5 cut(s) 206, 681, 980, 1013, 1119
BseAI TCCGGA 1 cut(s) 340
BseBI CCWGG 1 cut(s) 262
BseDI CCNNGG 2 cut(s) 96, 731
BseGI GGATG 4 cut(s) 52, 328, 353, 1123
BseLI CCNNNNNNNGG 3 cut(s) 102, 377, 1008
BseMII CTCAG 3 cut(s) 153, 540, 670
BseNI ACTGG 5 cut(s) 206, 681, 980, 1013, 1119
BseRI GAGGAG 1 cut(s) 994
BseXI GCAGC 3 cut(s) 784, 923, 1111
BsgI GTGCAG 1 cut(s) 258
Bsh1236I CGCG 1 cut(s) 855
BshFI GGCC 1 cut(s) 654
BshNI GGYRCC 1 cut(s) 302
BsiHKCI CYCGRG 1 cut(s) 906
BsiSI CCGG 3 cut(s) 341, 1034, 1047
BslFI GGGAC 1 cut(s) 888
BslI CCNNNNNNNGG 3 cut(s) 102, 377, 1008
BsmAI GTCTC 3 cut(s) 217, 378, 885
BsmBI CGTCTC 2 cut(s) 217, 378
BsmFI GGGAC 1 cut(s) 888
BsmI GAATGC 1 cut(s) 799
BsnI GGCC 1 cut(s) 654
BsoBI CYCGRG 1 cut(s) 906
Bsp119I TTCGAA 1 cut(s) 1080
Bsp13I TCCGGA 1 cut(s) 340
Bsp143I GATC 4 cut(s) 151, 349, 416, 990
Bsp19I CCATGG 2 cut(s) 96, 731
Bsp68I TCGCGA 1 cut(s) 855
BspACI CCGC 1 cut(s) 1055
BspANI GGCC 1 cut(s) 654
BspCNI CTCAG 3 cut(s) 154, 539, 669
BspEI TCCGGA 1 cut(s) 340
BspFNI CGCG 1 cut(s) 855
BspLI GGNNCC 2 cut(s) 304, 904
BspPI GGATC 2 cut(s) 344, 998
BspT104I TTCGAA 1 cut(s) 1080
BspT107I GGYRCC 1 cut(s) 302
BsrI ACTGG 5 cut(s) 206, 681, 980, 1013, 1119
BssECI CCNNGG 2 cut(s) 96, 731
BssMI GATC 4 cut(s) 151, 349, 416, 990
BssT1I CCWWGG 2 cut(s) 96, 731
Bst2UI CCWGG 1 cut(s) 262
Bst4CI ACNGT 2 cut(s) 372, 783
Bst6I CTCTTC 1 cut(s) 828
BstBI TTCGAA 1 cut(s) 1080
BstC8I GCNNGC 1 cut(s) 706
BstDEI CTNAG 4 cut(s) 162, 526, 582, 656
BstDSI CCRYGG 2 cut(s) 96, 731
BstF5I GGATG 4 cut(s) 52, 328, 353, 1123
BstFNI CGCG 1 cut(s) 855
BstH2I RGCGCY 1 cut(s) 1101
BstHHI GCGC 2 cut(s) 746, 1100
BstKTI GATC 4 cut(s) 154, 352, 419, 993
BstMAI GTCTC 3 cut(s) 217, 378, 885
BstMBI GATC 4 cut(s) 151, 349, 416, 990
BstMWI GCNNNNNNNGC 1 cut(s) 587
BstNI CCWGG 1 cut(s) 262
BstNSI RCATGY 2 cut(s) 491, 708
BstSCI CCNGG 2 cut(s) 260, 1032
BstUI CGCG 1 cut(s) 855
BstV1I GCAGC 3 cut(s) 784, 923, 1111
BstV2I GAAGAC 1 cut(s) 839
BsuRI GGCC 1 cut(s) 654
BtgI CCRYGG 2 cut(s) 96, 731
BtsCI GGATG 4 cut(s) 52, 328, 353, 1123
BtsIMutI CAGTG 2 cut(s) 294, 1112
BtuMI TCGCGA 1 cut(s) 855
Cac8I GCNNGC 1 cut(s) 706
CfoI GCGC 2 cut(s) 746, 1100
Cfr13I GGNCC 3 cut(s) 62, 728, 902
Csp6I GTAC 4 cut(s) 34, 466, 485, 1087
CviAII CATG 6 cut(s) 97, 157, 488, 705, 710, 732
CviQI GTAC 4 cut(s) 34, 466, 485, 1087
DdeI CTNAG 4 cut(s) 162, 526, 582, 656
DpnI GATC 4 cut(s) 153, 351, 418, 992
DpnII GATC 4 cut(s) 151, 349, 416, 990
DrdI GACNNNNNNGTC 1 cut(s) 231
DseDI GACNNNNNNGTC 1 cut(s) 231
Eam1104I CTCTTC 1 cut(s) 828
EarI CTCTTC 1 cut(s) 828
Eco130I CCWWGG 2 cut(s) 96, 731
Eco47I GGWCC 3 cut(s) 62, 728, 902
Eco47III AGCGCT 1 cut(s) 1099
Eco57I CTGAAG 3 cut(s) 446, 498, 626
Eco88I CYCGRG 1 cut(s) 906
EcoRII CCWGG 1 cut(s) 260
EcoT14I CCWWGG 2 cut(s) 96, 731
EcoT22I ATGCAT 1 cut(s) 493
ErhI CCWWGG 2 cut(s) 96, 731
Esp3I CGTCTC 2 cut(s) 217, 378
FaeI CATG 6 cut(s) 100, 160, 491, 708, 713, 735
FalI AAGNNNNNCTT 4 cut(s) 523, 555, 831, 863
FaqI GGGAC 1 cut(s) 888
FatI CATG 6 cut(s) 96, 156, 487, 704, 709, 731
Fnu4HI GCNGC 4 cut(s) 5, 773, 912, 1125
FokI GGATG 4 cut(s) 59, 335, 340, 1130
Fsp4HI GCNGC 4 cut(s) 5, 773, 912, 1125
FspBI CTAG 1 cut(s) 798
GlaI GCGC 2 cut(s) 745, 1099
GluI GCNGC 4 cut(s) 5, 773, 912, 1125
GsuI CTGGAG 2 cut(s) 283, 997
HaeII RGCGCY 1 cut(s) 1101
HaeIII GGCC 1 cut(s) 654
HapII CCGG 3 cut(s) 341, 1034, 1047
HhaI GCGC 2 cut(s) 746, 1100
Hin1II CATG 6 cut(s) 100, 160, 491, 708, 713, 735
Hin6I GCGC 2 cut(s) 744, 1098
HinP1I GCGC 2 cut(s) 744, 1098
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HindIII AAGCTT 2 cut(s) 573, 940
HinfI GANTC 2 cut(s) 440, 1109
HpaII CCGG 3 cut(s) 341, 1034, 1047
HphI GGTGA 3 cut(s) 991, 1048, 1069
Hpy166II GTNNAC 1 cut(s) 559
Hpy188I TCNGA 5 cut(s) 163, 478, 529, 606, 933
Hpy188III TCNNGA 5 cut(s) 60, 341, 411, 424, 854
Hpy8I GTNNAC 1 cut(s) 559
HpyAV CCTTC 3 cut(s) 116, 421, 601
HpyCH4III ACNGT 2 cut(s) 372, 783
HpyCH4IV ACGT 4 cut(s) 24, 226, 256, 345
HpyF10VI GCNNNNNNNGC 1 cut(s) 587
HpyF3I CTNAG 4 cut(s) 162, 526, 582, 656
HpySE526I ACGT 4 cut(s) 24, 226, 256, 345
Hsp92II CATG 6 cut(s) 100, 160, 491, 708, 713, 735
HspAI GCGC 2 cut(s) 744, 1098
Kpn2I TCCGGA 1 cut(s) 340
Kzo9I GATC 4 cut(s) 151, 349, 416, 990
Lsp1109I GCAGC 3 cut(s) 784, 923, 1111
LweI GCATC 3 cut(s) 37, 61, 65
MaeI CTAG 1 cut(s) 798
MaeII ACGT 4 cut(s) 24, 226, 256, 345
MaeIII GTNAC 1 cut(s) 1057
MalI GATC 4 cut(s) 153, 351, 418, 992
MboI GATC 4 cut(s) 151, 349, 416, 990
MboII GAAGA 7 cut(s) 426, 695, 713, 839, 842, 845, 982
MfeI CAATTG 1 cut(s) 66
MluCI AATT 2 cut(s) 66, 289
MmeI TCCRAC 2 cut(s) 88, 717
MnlI CCTC 6 cut(s) 465, 665, 829, 943, 972, 975
Mph1103I ATGCAT 1 cut(s) 493
MroI TCCGGA 1 cut(s) 340
MroXI GAANNNNTTC 1 cut(s) 1028
MseI TTAA 3 cut(s) 195, 207, 629
MslI CAYNNNNRTG 1 cut(s) 630
MspA1I CMGCKG 1 cut(s) 598
MspI CCGG 3 cut(s) 341, 1034, 1047
MspR9I CCNGG 2 cut(s) 262, 1034
MunI CAATTG 1 cut(s) 66
Mva1269I GAATGC 1 cut(s) 799
MvaI CCWGG 1 cut(s) 262
MvnI CGCG 1 cut(s) 855
MwoI GCNNNNNNNGC 1 cut(s) 587
NciI CCSGG 1 cut(s) 1034
NcoI CCATGG 2 cut(s) 96, 731
NdeII GATC 4 cut(s) 151, 349, 416, 990
NlaIII CATG 6 cut(s) 100, 160, 491, 708, 713, 735
NlaIV GGNNCC 2 cut(s) 304, 904
NmuCI GTSAC 1 cut(s) 1057
NruI TCGCGA 1 cut(s) 855
NsiI ATGCAT 1 cut(s) 493
NspI RCATGY 2 cut(s) 491, 708
NspV TTCGAA 1 cut(s) 1080
PaeI GCATGC 1 cut(s) 708
PcsI WCGNNNNNNNCGW 1 cut(s) 232
PctI GAATGC 1 cut(s) 799
PdmI GAANNNNTTC 1 cut(s) 1028
PfeI GAWTC 2 cut(s) 440, 1109
PflMI CCANNNNNTGG 1 cut(s) 1008
PkrI GCNGC 4 cut(s) 6, 774, 913, 1126
PshBI ATTAAT 1 cut(s) 207
Psp1406I AACGTT 1 cut(s) 24
Psp6I CCWGG 1 cut(s) 260
PspGI CCWGG 1 cut(s) 260
PspN4I GGNNCC 2 cut(s) 304, 904
PspPI GGNCC 3 cut(s) 62, 728, 902
PvuII CAGCTG 1 cut(s) 598
RruI TCGCGA 1 cut(s) 855
RsaI GTAC 4 cut(s) 35, 467, 486, 1088
RsaNI GTAC 4 cut(s) 34, 466, 485, 1087
RseI CAYNNNNRTG 1 cut(s) 630
SaqAI TTAA 3 cut(s) 195, 207, 629
SatI GCNGC 4 cut(s) 5, 773, 912, 1125
Sau3AI GATC 4 cut(s) 151, 349, 416, 990
Sau96I GGNCC 3 cut(s) 62, 728, 902
ScaI AGTACT 1 cut(s) 1088
ScrFI CCNGG 2 cut(s) 262, 1034
SfaNI GCATC 3 cut(s) 37, 61, 65
SfuI TTCGAA 1 cut(s) 1080
SinI GGWCC 3 cut(s) 62, 728, 902
SmiMI CAYNNNNRTG 1 cut(s) 630
SmlI CTYRAG 1 cut(s) 375
SmoI CTYRAG 1 cut(s) 375
SphI GCATGC 1 cut(s) 708
Sse9I AATT 2 cut(s) 66, 289
SsiI CCGC 1 cut(s) 1055
SspMI CTAG 1 cut(s) 798
StyD4I CCNGG 2 cut(s) 260, 1032
StyI CCWWGG 2 cut(s) 96, 731
TaaI ACNGT 2 cut(s) 372, 783
TaiI ACGT 4 cut(s) 27, 229, 259, 348
TaqI TCGA 2 cut(s) 470, 1080
TasI AATT 2 cut(s) 66, 289
TatI WGTACW 2 cut(s) 484, 1086
TfiI GAWTC 2 cut(s) 440, 1109
Tru1I TTAA 3 cut(s) 195, 207, 629
Tru9I TTAA 3 cut(s) 195, 207, 629
TscAI CASTG 2 cut(s) 301, 1119
TseFI GTSAC 1 cut(s) 1057
TseI GCWGC 4 cut(s) 4, 772, 911, 1124
Tsp45I GTSAC 1 cut(s) 1057
TspDTI ATGAA 3 cut(s) 432, 534, 1134
TspRI CASTG 2 cut(s) 301, 1119
Van91I CCANNNNNTGG 1 cut(s) 1008
VpaK11BI GGWCC 3 cut(s) 62, 728, 902
VspI ATTAAT 1 cut(s) 207
XceI RCATGY 2 cut(s) 491, 708
XcmI CCANNNNNNNNNTGG 1 cut(s) 72
XmnI GAANNNNTTC 1 cut(s) 1028
XspI CTAG 1 cut(s) 798
ZrmI AGTACT 1 cut(s) 1088
Zsp2I ATGCAT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.