Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0000933

Physical Location & Seq

Genomic Coordinates Forward (+)

40575544 .. 40585888

Gene Structure 10345 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000029569

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2412 bp

ATGTCGAATTCCATAACCCAGTTGATGATTCTGATTCTTGTCTTTATACCGATAGCTGTTTCAGAACCACAACAACCAGTTGAGGCTTTGCTGCGTCGTCTGGACTCCAAACGCTCCTCTGCTTCAGTTCAAGAAGCTGCAGCTAAAGCTGTTCTCAAACGATTGCTGCCTACCCATGTCGACAGTTTCGACTTCAAGATTGTTGACAAGGATGTTTGTGGTGGACATAGTTGCTTTGTGATAAACAATCACAACCTGTCAAGTCGAAATGGCCCCGAGATACAGATTAAAGGTACCACAGCAGTTGAAATTACTTCTGGCCTCCATTGGTATTTGAAGTATTGGTGTGGTGCTCATGTTTCTTGGGACAAGACTGGTGGCGTGCAATTAGCTTCCATCCCCAATCCAGGATCACTACCTCGTGTAAAAGACAAGGGGTTGAAAATTCAACGGCCGGTCCCATGGAACTATTACCAAAATGTCGTTACATCCAGTTATTCATTTGTTTGGTGGGATTGGGAAAGATGGCAGAAAGAAATAGACTGGATGGCTCTTCAGGGGATCAACCTGCCTTTAGCATTCACAGGGCAAGAATCAATTTGGCAGAAAGTTTTCATGGATTTCAATATTAGTAAGGGAGACTTGAATGATTTCTTTGGTGGACCTGCCTTCCTTGCTTGGGCTCGCATGGGGAATTTACATGCGTGGGGTGGACCATTATCACAGAATTGGTTGGATCAACAATTGGTTTTACAGAAACAGATATTATCCCAGATGTTAGATCTAGGCATGACTCCAGTGCTGCCGTCATTCTCTGGAAATGTCCCAGCAACACTGAAGAAATTTTATCCTTCAGCTAATGTAACCAGGCTTGGAGACTGGAACACTGTCAATGGTGATCGCCGCTGGTGCTGTACTTACCTTCTTGATCCTTCTGATCCTTTATTTGTTGAAATTGGGACTGCTTTTATTAGGCGACAAGTTGAAGAATACGGGGATGTGACAGACATCTATAACTGTGATACATTCAATGAAAATTCTCCGCCTACAAATGATCCAGCTTATATTTCTTCACTTGGAGCTGCTGTATACAAAGCAATGTCCAAAGGTGATACAGATGCAGTGTGGTTGATGCAAGGTTGGCTCTTCTACTCAGACTCTTCTTTTTGGAAACCACCTCAAATGAAGGCTCTTCTACATTCTGTTCCATTCGGGAAAATGATTGTACTTGATCTATTTGCTGATGTGAAACCAATATGGAACACGTCATCTCAATTTTACGACACTCCCTATATCTGGTGTCTCCTGCACAATTTTGGTGGGAACCTTGAAATGTATGGGATATTGGATGCAATTTCTTCAGGTCCAGTTGATGCCCGAACTAGTGAAAATTCTACTATGGTTGGGGTTGGCATGTGCATGGAAGGAATCGAGCACAATCCAGTTATCTATGAATTGACGTCTGAAATGGCATTTCGCAGTGAAAGGGTTCCAGTTCAGGATTGGCTGAGGAACTACTCCCATAGACGTTATGGTAATGCAGTTCATCAAGTTGAGGAGGCTTGGGAAATTCTTCATCGCACAATTTATAACTGCACAGATGGAATTGCTGACCATAACACAGATTTCATAGTCAAATTACCTGACTGGGATCCATCACTAGAGAGTGTATCGAACACGTCAGAACCTAACAAGATGCAAATGCTTTTCACACTAGATATGAAGAGGAGATTTTTACTCCAAAAAACCAGCTCTCAATTTCCTAAGGCACATTTGTGGTATTCAACTCAGGATGTGATTAATGCTTTACGGCTATTCCTTGATGCTGGAAATGATCTTTCCGGAAGCCTTACGTACAGATATGATTTGGTTGATTTAACACGCCAAGTGCTGTCAAAGCTAGCAAACCAAGTATATGTGAATGCAGTGACTGCTTTTCAGAGGAAGGATGTAAAAGCTTTTGATCTCAATAGTAAAAAGTTTGTTCAACTCATAAGGGATATTGATGTACTTCTTGCTTCTGATGATAATTTTTTACTTGGAACTTGGCTTGAAAGTGCAAAAAAGCTGGCAACCAGTCCTACGGAAATGAGGCAGTATGAGTGGAATGCAAGAACGCAGGTGACAATGTGGTATGATACCACAAAAACCAACCAGAGCCAGCTTCATGATTATGCAAATAAGTTCTGGAGTGGCCTATTGGAAAGTTACTATCTACCACGAGCTTCAACCTACTTTCATTATTTGTCAAAAAGCTTAAGAGAAAATAAAGACTTTGAAGTGGAAAGATGGAGAATAGAATGGATATCCTTTTCAAACAATTGGCAAGCAGGTACAGAGCTTTACCCAGTGAAGGCAAAAGGAGATGCACTTGCTATTTCTAAAGCATTGTATGAAAAATATTTCGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051302 GO:0051781 GO:0061458 GO:0065007

KEGG Pathways

Metabolic & Signaling

ko00531 ko01100 ko04142 map00531 map01100 map04142

Pfam Domains

Protein Families

NAGLU NAGLU_C NAGLU_N

Protein Analysis

804

Amino Acids

91.97

Weight (kDa)

6.02

Isoelectric Point (pI)

43.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAGLU_N	PF12971	46 - 140	1.3e-23	Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain
NAGLU	PF05089	157 - 493	6.1e-145	Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
NAGLU_C	PF12972	502 - 800	1.1e-90	Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000933)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13690 AT5G13690
fragaria_vesca	FvH4_1g18460 FvH4_1g19570 FvH4_1g19580 FvH4_5g16380 FvH4_5g16380 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470
malus_domestica	MD07G1018500.v1.1 MD07G1018700.v1.1 MD14G1187100.v1.1
prunus_persica	Prupe.2G020300_v2.0.a1 Prupe.2G020300_v2.0.a1 Prupe.2G020300_v2.0.a1 Prupe.2G020300_v2.0.a1 Prupe.5G179300_v2.0.a1 Prupe.5G179300_v2.0.a1
pyrus_communis	pycom07g01480 pycom14g15500
rosa_chinensis	RchiOBHm_Chr1g0317311 RchiOBHm_Chr7g0179731 RchiOBHm_Chr7g0179741
rosa_laevigata	RLG00000005326 RLG00000017711 RLG00000029569 RLG00000030607
rosa_multiflora	Rmu_sc0000186.1_g000002 Rmu_sc0004156.1_g000003 Rmu_sc0005092.1_g000001 Rmu_sc0013869.1_g000005 Rmu_sc0026282.1_g000001
rosa_roxburghii	Rroxscaffold_3G00273510 Rroxscaffold_4G00330500
rosa_rugosa	Rorug01G0015800 Rorug02G0157200 Rorug06G0427700
rosa_samantha	Rh1AG027200 Rh1AG093700 Rh1BG023100 Rh1CG025200 Rh1DG046000 Rh2AG208700 Rh4CG293700 Rh7AG027200 Rh7AG027300 Rh7BG026300 Rh7BG026400 Rh7CG028400 Rh7CG170000 Rh7DG028000
rosa_wichuraiana	Rw1G001880 Rw7G002190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1590
AarI	CACCTGC	1 cut(s)	2110
AatII	GACGTC	1 cut(s)	1463
Acc36I	ACCTGC	4 cut(s)	576, 673, 2110, 2321
Acc65I	GGTACC	1 cut(s)	293
AccB1I	GGYRCC	1 cut(s)	293
AccI	GTMKAC	2 cut(s)	180, 1089
AccIII	TCCGGA	1 cut(s)	1841
AciI	CCGC	2 cut(s)	904, 1043
AclWI	GGATC	8 cut(s)	418, 569, 744, 923, 932, 1049, 1646, 1659
AcoI	YGGCCR	1 cut(s)	452
AcsI	RAATTY	7 cut(s)	7, 444, 694, 842, 1036, 1390, 1569
AcuI	CTGAAG	5 cut(s)	108, 539, 837, 857, 1344
AcyI	GRCGYC	1 cut(s)	1460
AfaI	GTAC	6 cut(s)	295, 916, 1227, 1856, 2010, 2335
AfiI	CCNNNNNNNGG	4 cut(s)	407, 679, 1296, 2353
AflII	CTTAAG	1 cut(s)	2257
AflIII	ACRYGT	2 cut(s)	1263, 1677
AhlI	ACTAGT	1 cut(s)	1382
AjiI	CACGTC	2 cut(s)	1266, 1680
AjnI	CCWGG	2 cut(s)	406, 866
AleI	CACNNNNGTG	1 cut(s)	1774
Alw21I	GWGCWC	2 cut(s)	355, 1437
Alw26I	GTCTC	3 cut(s)	633, 870, 1307
AlwI	GGATC	8 cut(s)	418, 569, 744, 923, 932, 1049, 1646, 1659
AlwNI	CAGNNNCTG	1 cut(s)	1931
Ama87I	CYCGRG	1 cut(s)	275
Aor13HI	TCCGGA	1 cut(s)	1841
AoxI	GGCC	4 cut(s)	271, 319, 452, 2194
ApeKI	GCWGC	6 cut(s)	91, 137, 140, 166, 802, 1082
ApoI	RAATTY	7 cut(s)	7, 444, 694, 842, 1036, 1390, 1569
AseI	ATTAAT	1 cut(s)	1800
Asp700I	GAANNNNTTC	4 cut(s)	611, 650, 1488, 1572
Asp718I	GGTACC	1 cut(s)	293
AspS9I	GGNCC	5 cut(s)	272, 457, 662, 713, 1364
AsuHPI	GGTGA	3 cut(s)	908, 1121, 2134
AsuNHI	GCTAGC	1 cut(s)	1900
AvaI	CYCGRG	1 cut(s)	275
AvaII	GGWCC	4 cut(s)	457, 662, 713, 1364
AxyI	CCTNAGG	1 cut(s)	1764
BamHI	GGATCC	1 cut(s)	1651
BanI	GGYRCC	1 cut(s)	293
BanII	GRGCYC	1 cut(s)	685
BauI	CACGAG	2 cut(s)	420, 2220
Bbv12I	GWGCWC	2 cut(s)	355, 1437
BbvCI	CCTCAGC	1 cut(s)	1508
BbvI	GCAGC	6 cut(s)	78, 124, 152, 153, 789, 1069
BccI	CCATC	6 cut(s)	404, 519, 541, 1595, 1663, 2283
BceAI	ACGGC	3 cut(s)	467, 790, 1826
BciT130I	CCWGG	2 cut(s)	408, 868
BcoDI	GTCTC	3 cut(s)	633, 870, 1307
BcuI	ACTAGT	1 cut(s)	1382
BfaI	CTAG	5 cut(s)	785, 1383, 1661, 1715, 1901
BfmI	CTRYAG	1 cut(s)	138
BfrI	CTTAAG	1 cut(s)	2257
BfuAI	ACCTGC	4 cut(s)	576, 673, 2110, 2321
BglII	AGATCT	1 cut(s)	781
BisI	GCNGC	7 cut(s)	92, 138, 141, 167, 803, 904, 1083
BlsI	GCNGC	7 cut(s)	93, 139, 142, 168, 804, 905, 1084
Bme1390I	CCNGG	2 cut(s)	408, 868
Bme18I	GGWCC	4 cut(s)	457, 662, 713, 1364
BmeT110I	CYCGRG	1 cut(s)	275
BmgBI	CACGTC	2 cut(s)	1266, 1680
BmgT120I	GGNCC	5 cut(s)	272, 457, 662, 713, 1364
BmiI	GGNNCC	6 cut(s)	274, 295, 459, 1325, 1491, 1653
BmrFI	CCNGG	2 cut(s)	408, 868
BmrI	ACTGGG	3 cut(s)	13, 1657, 2342
BmsI	GCATC	7 cut(s)	1108, 1122, 1339, 1363, 1686, 1813, 2356
BmtI	GCTAGC	1 cut(s)	1904
BmuI	ACTGGG	3 cut(s)	13, 1657, 2342
BpmI	CTGGAG	2 cut(s)	780, 2209
Bpu10I	CCTNAGC	1 cut(s)	1508
BsaAI	YACGTR	1 cut(s)	1854
BsaHI	GRCGYC	1 cut(s)	1460
BsaJI	CCNNGG	1 cut(s)	461
BsaWI	WCCGGW	1 cut(s)	1841
BsaXI	ACNNNNNCTCC	2 cut(s)	1071, 1101
Bsc4I	CCNNNNNNNGG	4 cut(s)	407, 679, 1296, 2353
Bse118I	RCCGGY	1 cut(s)	454
Bse21I	CCTNAGG	1 cut(s)	1764
Bse3DI	GCAATG	1 cut(s)	1104
BseAI	TCCGGA	1 cut(s)	1841
BseBI	CCWGG	2 cut(s)	408, 868
BseDI	CCNNGG	1 cut(s)	461
BseGI	GGATG	8 cut(s)	217, 396, 488, 552, 1003, 1354, 1798, 1954
BseLI	CCNNNNNNNGG	4 cut(s)	407, 679, 1296, 2353
BseMI	GCAATG	1 cut(s)	1104
BseMII	CTCAG	3 cut(s)	1167, 1499, 1802
BseRI	GAGGAG	3 cut(s)	106, 1571, 1741
BseX3I	CGGCCG	1 cut(s)	452
BseXI	GCAGC	6 cut(s)	78, 124, 152, 153, 789, 1069
BseYI	CCCAGC	1 cut(s)	826
BsgI	GTGCAG	2 cut(s)	1292, 1579
Bsh1285I	CGRYCG	1 cut(s)	455
BshFI	GGCC	4 cut(s)	273, 321, 454, 2196
BshNI	GGYRCC	1 cut(s)	293
BsiEI	CGRYCG	1 cut(s)	455
BsiHKAI	GWGCWC	2 cut(s)	355, 1437
BsiHKCI	CYCGRG	1 cut(s)	275
BsiSI	CCGG	2 cut(s)	455, 1842
BslFI	GGGAC	4 cut(s)	380, 443, 809, 973
BslI	CCNNNNNNNGG	4 cut(s)	407, 679, 1296, 2353
BsmAI	GTCTC	3 cut(s)	633, 870, 1307
BsmFI	GGGAC	4 cut(s)	380, 443, 809, 973
BsmI	GAATGC	3 cut(s)	578, 1927, 2113
BsnI	GGCC	4 cut(s)	273, 321, 454, 2196
BsoBI	CYCGRG	1 cut(s)	275
Bsp1286I	GDGCHC	3 cut(s)	355, 685, 1437
Bsp13I	TCCGGA	1 cut(s)	1841
Bsp19I	CCATGG	1 cut(s)	461
BspACI	CCGC	2 cut(s)	904, 1043
BspANI	GGCC	4 cut(s)	273, 321, 454, 2196
BspCNI	CTCAG	3 cut(s)	1166, 1500, 1801
BspEI	TCCGGA	1 cut(s)	1841
BspHI	TCATGA	1 cut(s)	2167
BspLI	GGNNCC	6 cut(s)	274, 295, 459, 1325, 1491, 1653
BspMAI	CTGCAG	1 cut(s)	142
BspMI	ACCTGC	4 cut(s)	576, 673, 2110, 2321
BspOI	GCTAGC	1 cut(s)	1904
BspPI	GGATC	8 cut(s)	418, 569, 744, 923, 932, 1049, 1646, 1659
BspQI	GCTCTTC	3 cut(s)	558, 1151, 1197
BspT107I	GGYRCC	1 cut(s)	293
BspTI	CTTAAG	1 cut(s)	2257
BsrDI	GCAATG	1 cut(s)	1104
BsrFI	RCCGGY	1 cut(s)	454
BssAI	RCCGGY	1 cut(s)	454
BssECI	CCNNGG	1 cut(s)	461
BssNAI	GTATAC	1 cut(s)	1090
BssNI	GRCGYC	1 cut(s)	1460
BssSI	CACGAG	2 cut(s)	420, 2220
BssT1I	CCWWGG	1 cut(s)	461
Bst1107I	GTATAC	1 cut(s)	1090
Bst2BI	CACGAG	2 cut(s)	420, 2220
Bst2UI	CCWGG	2 cut(s)	408, 868
Bst4CI	ACNGT	3 cut(s)	185, 889, 1019
Bst6I	CTCTTC	5 cut(s)	558, 1151, 1165, 1197, 1718
BstACI	GRCGYC	1 cut(s)	1460
BstAFI	CTTAAG	1 cut(s)	2257
BstAPI	GCANNNNNTGC	1 cut(s)	1931
BstBAI	YACGTR	1 cut(s)	1854
BstC8I	GCNNGC	6 cut(s)	383, 685, 1902, 2070, 2162, 2328
BstDEI	CTNAG	4 cut(s)	1153, 1508, 1764, 1788
BstDSI	CCRYGG	1 cut(s)	461
BstF5I	GGATG	8 cut(s)	217, 396, 488, 552, 1003, 1354, 1798, 1954
BstMAI	GTCTC	3 cut(s)	633, 870, 1307
BstMCI	CGRYCG	1 cut(s)	455
BstMWI	GCNNNNNNNGC	6 cut(s)	146, 674, 909, 1141, 1897, 1931
BstNI	CCWGG	2 cut(s)	408, 868
BstNSI	RCATGY	2 cut(s)	704, 1417
BstSCI	CCNGG	2 cut(s)	406, 866
BstSFI	CTRYAG	1 cut(s)	138
BstSNI	TACGTA	1 cut(s)	1854
BstV1I	GCAGC	6 cut(s)	78, 124, 152, 153, 789, 1069
BstX2I	RGATCY	2 cut(s)	781, 1651
BstYI	RGATCY	2 cut(s)	781, 1651
BstZ17I	GTATAC	1 cut(s)	1090
BstZI	CGGCCG	1 cut(s)	452
Bsu36I	CCTNAGG	1 cut(s)	1764
BsuRI	GGCC	4 cut(s)	273, 321, 454, 2196
BtgI	CCRYGG	1 cut(s)	461
BtgZI	GCGATG	1 cut(s)	1562
BtrI	CACGTC	2 cut(s)	1266, 1680
BtsCI	GGATG	8 cut(s)	217, 396, 488, 552, 1003, 1354, 1798, 1954
BtsI	GCAGTG	3 cut(s)	1128, 1486, 1932
BtsIMutI	CAGTG	7 cut(s)	804, 833, 885, 1128, 1486, 1932, 2355
BveI	ACCTGC	4 cut(s)	576, 673, 2110, 2321
Cac8I	GCNNGC	6 cut(s)	383, 685, 1902, 2070, 2162, 2328
CaiI	CAGNNNCTG	1 cut(s)	1931
CciI	TCATGA	1 cut(s)	2167
Cfr10I	RCCGGY	1 cut(s)	454
Cfr13I	GGNCC	5 cut(s)	272, 457, 662, 713, 1364
CseI	GACGC	1 cut(s)	83
Csp6I	GTAC	6 cut(s)	294, 915, 1226, 1855, 2009, 2334
CspCI	CAANNNNNGTGG	6 cut(s)	286, 321, 358, 393, 1300, 1335
CviQI	GTAC	6 cut(s)	294, 915, 1226, 1855, 2009, 2334
DdeI	CTNAG	4 cut(s)	1153, 1508, 1764, 1788
EaeI	YGGCCR	1 cut(s)	452
EagI	CGGCCG	1 cut(s)	452
Eam1104I	CTCTTC	5 cut(s)	558, 1151, 1165, 1197, 1718
EarI	CTCTTC	5 cut(s)	558, 1151, 1165, 1197, 1718
EciI	GGCGGA	1 cut(s)	1032
EclXI	CGGCCG	1 cut(s)	452
Eco105I	TACGTA	1 cut(s)	1854
Eco130I	CCWWGG	1 cut(s)	461
Eco24I	GRGCYC	1 cut(s)	685
Eco32I	GATATC	1 cut(s)	2307
Eco47I	GGWCC	4 cut(s)	457, 662, 713, 1364
Eco52I	CGGCCG	1 cut(s)	452
Eco57I	CTGAAG	5 cut(s)	108, 539, 837, 857, 1344
Eco81I	CCTNAGG	1 cut(s)	1764
Eco88I	CYCGRG	1 cut(s)	275
EcoRI	GAATTC	1 cut(s)	7
EcoRII	CCWGG	2 cut(s)	406, 866
EcoRV	GATATC	1 cut(s)	2307
EcoT14I	CCWWGG	1 cut(s)	461
EcoT38I	GRGCYC	1 cut(s)	685
ErhI	CCWWGG	1 cut(s)	461
FalI	AAGNNNNNCTT	2 cut(s)	626, 658
FaqI	GGGAC	4 cut(s)	380, 443, 809, 973
FblI	GTMKAC	2 cut(s)	180, 1089
Fnu4HI	GCNGC	7 cut(s)	92, 138, 141, 167, 803, 904, 1083
FokI	GGATG	8 cut(s)	224, 383, 475, 559, 1010, 1361, 1805, 1961
FriOI	GRGCYC	1 cut(s)	685
Fsp4HI	GCNGC	7 cut(s)	92, 138, 141, 167, 803, 904, 1083
FspBI	CTAG	5 cut(s)	785, 1383, 1661, 1715, 1901
GluI	GCNGC	7 cut(s)	92, 138, 141, 167, 803, 904, 1083
GsaI	CCCAGC	1 cut(s)	830
GsuI	CTGGAG	2 cut(s)	780, 2209
HaeIII	GGCC	4 cut(s)	273, 321, 454, 2196
HapII	CCGG	2 cut(s)	455, 1842
HgaI	GACGC	1 cut(s)	83
Hin1I	GRCGYC	1 cut(s)	1460
HincII	GTYRAC	2 cut(s)	181, 205
HindII	GTYRAC	2 cut(s)	181, 205
HindIII	AAGCTT	2 cut(s)	1956, 2254
HinfI	GANTC	7 cut(s)	28, 34, 104, 593, 793, 1157, 1428
HpaII	CCGG	2 cut(s)	455, 1842
HphI	GGTGA	3 cut(s)	908, 1121, 2134
Hpy166II	GTNNAC	6 cut(s)	181, 205, 224, 662, 713, 1090
Hpy188I	TCNGA	8 cut(s)	33, 64, 937, 1156, 1465, 1684, 1941, 2023
Hpy8I	GTNNAC	6 cut(s)	181, 205, 224, 662, 713, 1090
Hpy99I	CGWCG	1 cut(s)	99
HpyAV	CCTTC	8 cut(s)	679, 861, 932, 942, 1180, 1418, 1939, 2347
HpyCH4III	ACNGT	3 cut(s)	185, 889, 1019
HpyCH4IV	ACGT	5 cut(s)	1265, 1460, 1528, 1679, 1853
HpyF10VI	GCNNNNNNNGC	6 cut(s)	146, 674, 909, 1141, 1897, 1931
HpyF3I	CTNAG	4 cut(s)	1153, 1508, 1764, 1788
HpySE526I	ACGT	5 cut(s)	1265, 1460, 1528, 1679, 1853
Hsp92I	GRCGYC	1 cut(s)	1460
Kpn2I	TCCGGA	1 cut(s)	1841
KpnI	GGTACC	1 cut(s)	297
LguI	GCTCTTC	3 cut(s)	558, 1151, 1197
LmnI	GCTCC	2 cut(s)	119, 1079
Lsp1109I	GCAGC	6 cut(s)	78, 124, 152, 153, 789, 1069
LweI	GCATC	7 cut(s)	1108, 1122, 1339, 1363, 1686, 1813, 2356
MaeI	CTAG	5 cut(s)	785, 1383, 1661, 1715, 1901
MaeII	ACGT	5 cut(s)	1265, 1460, 1528, 1679, 1853
MaeIII	GTNAC	6 cut(s)	484, 862, 1000, 1927, 2122, 2207
MfeI	CAATTG	2 cut(s)	743, 2320
MflI	RGATCY	2 cut(s)	781, 1651
MhlI	GDGCHC	3 cut(s)	355, 685, 1437
MlyI	GAGTC	3 cut(s)	98, 787, 1151
MmeI	TCCRAC	1 cut(s)	714
MroI	TCCGGA	1 cut(s)	1841
MroXI	GAANNNNTTC	4 cut(s)	611, 650, 1488, 1572
MseI	TTAA	4 cut(s)	288, 1800, 1877, 2258
MslI	CAYNNNNRTG	3 cut(s)	1418, 1774, 2172
MspA1I	CMGCKG	1 cut(s)	906
MspCI	CTTAAG	1 cut(s)	2257
MspI	CCGG	2 cut(s)	455, 1842
MspR9I	CCNGG	2 cut(s)	408, 868
MunI	CAATTG	2 cut(s)	743, 2320
Mva1269I	GAATGC	3 cut(s)	578, 1927, 2113
MvaI	CCWGG	2 cut(s)	408, 868
MwoI	GCNNNNNNNGC	6 cut(s)	146, 674, 909, 1141, 1897, 1931
NcoI	CCATGG	1 cut(s)	461
NheI	GCTAGC	1 cut(s)	1900
NlaIV	GGNNCC	6 cut(s)	274, 295, 459, 1325, 1491, 1653
NmuCI	GTSAC	3 cut(s)	1000, 1927, 2122
NspI	RCATGY	2 cut(s)	704, 1417
OliI	CACNNNNGTG	1 cut(s)	1774
PagI	TCATGA	1 cut(s)	2167
PaqCI	CACCTGC	1 cut(s)	2110
PciSI	GCTCTTC	3 cut(s)	558, 1151, 1197
PcsI	WCGNNNNNNNCGW	1 cut(s)	186
PctI	GAATGC	3 cut(s)	578, 1927, 2113
PdmI	GAANNNNTTC	4 cut(s)	611, 650, 1488, 1572
PfeI	GAWTC	4 cut(s)	28, 34, 593, 1428
PfoI	TCCNGGA	1 cut(s)	406
PkrI	GCNGC	7 cut(s)	93, 139, 142, 168, 804, 905, 1084
PleI	GAGTC	3 cut(s)	98, 787, 1151
PpsI	GAGTC	3 cut(s)	98, 787, 1151
Ppu21I	YACGTR	1 cut(s)	1854
PshBI	ATTAAT	1 cut(s)	1800
PsiI	TTATAA	1 cut(s)	1590
Psp6I	CCWGG	2 cut(s)	406, 866
PspFI	CCCAGC	1 cut(s)	826
PspGI	CCWGG	2 cut(s)	406, 866
PspN4I	GGNNCC	6 cut(s)	274, 295, 459, 1325, 1491, 1653
PspPI	GGNCC	5 cut(s)	272, 457, 662, 713, 1364
PstI	CTGCAG	1 cut(s)	142
PstNI	CAGNNNCTG	1 cut(s)	1931
PsuI	RGATCY	2 cut(s)	781, 1651
RsaI	GTAC	6 cut(s)	295, 916, 1227, 1856, 2010, 2335
RsaNI	GTAC	6 cut(s)	294, 915, 1226, 1855, 2009, 2334
RseI	CAYNNNNRTG	3 cut(s)	1418, 1774, 2172
SalI	GTCGAC	1 cut(s)	179
SapI	GCTCTTC	3 cut(s)	558, 1151, 1197
SaqAI	TTAA	4 cut(s)	288, 1800, 1877, 2258
SatI	GCNGC	7 cut(s)	92, 138, 141, 167, 803, 904, 1083
Sau96I	GGNCC	5 cut(s)	272, 457, 662, 713, 1364
SchI	GAGTC	3 cut(s)	98, 787, 1151
ScrFI	CCNGG	2 cut(s)	408, 868
SduI	GDGCHC	3 cut(s)	355, 685, 1437
SfaNI	GCATC	7 cut(s)	1108, 1122, 1339, 1363, 1686, 1813, 2356
SfcI	CTRYAG	1 cut(s)	138
SinI	GGWCC	4 cut(s)	457, 662, 713, 1364
SmiMI	CAYNNNNRTG	3 cut(s)	1418, 1774, 2172
SmlI	CTYRAG	1 cut(s)	2257
SmoI	CTYRAG	1 cut(s)	2257
SnaBI	TACGTA	1 cut(s)	1854
SpeI	ACTAGT	1 cut(s)	1382
SsiI	CCGC	2 cut(s)	904, 1043
SspI	AATATT	2 cut(s)	628, 2402
SspMI	CTAG	5 cut(s)	785, 1383, 1661, 1715, 1901
StyD4I	CCNGG	2 cut(s)	406, 866
StyI	CCWWGG	1 cut(s)	461
TaaI	ACNGT	3 cut(s)	185, 889, 1019
TaiI	ACGT	5 cut(s)	1268, 1463, 1531, 1682, 1856
TaqI	TCGA	6 cut(s)	5, 180, 189, 265, 1431, 1673
TatI	WGTACW	3 cut(s)	914, 1225, 2008
TauI	GCSGC	1 cut(s)	906
TfiI	GAWTC	4 cut(s)	28, 34, 593, 1428
Tru1I	TTAA	4 cut(s)	288, 1800, 1877, 2258
Tru9I	TTAA	4 cut(s)	288, 1800, 1877, 2258
TscAI	CASTG	7 cut(s)	804, 840, 892, 1128, 1486, 1932, 2355
TseFI	GTSAC	3 cut(s)	1000, 1927, 2122
TseI	GCWGC	6 cut(s)	91, 137, 140, 166, 802, 1082
Tsp45I	GTSAC	3 cut(s)	1000, 1927, 2122
TspGWI	ACGGA	1 cut(s)	2099
TspRI	CASTG	7 cut(s)	804, 840, 892, 1128, 1486, 1932, 2355
Vha464I	CTTAAG	1 cut(s)	2257
VpaK11BI	GGWCC	4 cut(s)	457, 662, 713, 1364
VspI	ATTAAT	1 cut(s)	1800
XapI	RAATTY	7 cut(s)	7, 444, 694, 842, 1036, 1390, 1569
XceI	RCATGY	2 cut(s)	704, 1417
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1500, 1529
XmiI	GTMKAC	2 cut(s)	180, 1089
XmnI	GAANNNNTTC	4 cut(s)	611, 650, 1488, 1572
XspI	CTAG	5 cut(s)	785, 1383, 1661, 1715, 1901
ZraI	GACGTC	1 cut(s)	1461

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.