RLG00000005326

alpha-N-acetylglucosaminidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000933
Physical Location & Seq
Reverse (-)
66807138 .. 66812568
5431 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005326

Sequence Viewer

Length: 2508 bp
ATGGCTCCCACCACATTTCCCGCCATTTCTACCATTACCATCTTCACGCTCTTCTTCTTCTTCACCTTGTGTGTCCTCTCCTCATCATCATCCACCATCGGAGTCGGCTACATATCGAGCGTTCTGGAACTTCAGGACCGCGAGAGGGCCTCTCCCTATGTCCAAGTCGCCGCCGCTCGCGGAGTACTCCGCCGCTTTCTTCCTTCCCAATCCTCCAGCTTCGACTTCCAAATCGTCTCTAAGGAGCAATGTGGTGGTGCATCCTGCTTTATGATTAAGAACCATCCTTCTTTTCGTAGACGAGGGGATCCACAGATTCTGATTTCCGGAGTCACTGGAGTGGAGATTTTGGCTGGTCTGCATTGGTATTTGAAGAATTGGTGTGGCTCACATATATCTTGGGATAAAACTGGTGGTGCGCAACTATTTTCAGTCCCAAAAGCAGGGTTTCTCTCTCGTGTAGGAGATGGTGGCATTTTAGTCCAGAGACCTGTTCCTTGGAACTATTACCAGAATGCTGTCACATCTAGCTATTCTTTGGCTTGGTGGGACTGGAATAGATGGGAAAAGGAGATTGATATGATGGCCCTCCAGGGTATCAATCTGCCACTAGCATTTACAGGTCAAGAGGCTATATGGCAGAAAGTTTTCCAGAAATTTAATATGAGCAAATCAGATTTGGATGATTTCTTTGGAGGCCCTGCTTTCCTTGCATGGTCTCGAATGGGAAATTTGCATGGATGGGGTGGACCTCTCCCGCAAAGTTGGCTTGATCAACAGCTGATTCTGCAGAAAAAAATTCTCAACAGAATGTATGACCTTGGAATGACTCCAGTCCTTCCAGCATTTTCTGGGAATGTTCCTGCAGCACTCAAAACTATATATCCATCAGCAAAGATTACACAGTTAGGAAACTGGTTTTCTGTTAAGAGTGACCCTAGATGGACCTGCACTTACCTTCTTGATGCAACTGATCCCTTATTTGTTGAAATTGGGAAAACATTCATTGAGGAACAACTAAAAGAATATGGAAGGACCAGTCACATTTACAACTGTGATACTTTTGATGAGAATACTCCACCTGTTGATGATCCAGAGTACATCTCTGCATTAGGTAAAACAATATTTAAGGGACTGCAAAGTGGAGATAAGGACGGTGTATGGCTAATGCAGGGATGGTTATTTTCATATGATCCATTCTGGAGGCCTGCACAGATGAAGGCACTCTTACATTCTGTTCCTGCAGGGAGGATGGTAGTCCTTGATCTGTTTGCTGAAGTGAAACCGATATGGACTACTTCAGAGCAGTTTTATGGTGTTCCTTACATCTGGAAAATCCTTACTTACTTTGTTATTCATTACAGGTGTATGCTGCACAACTTTGCAGGAAACGTTGAGATGTACGGCGTTATGGATGCAATAGCATCTGGACCAATTGATGCTTGGACAAGTGAAAACTCAACCATGGTTGGGGTTGGAATGTCAATGGAAGGTATAGAACAGAACCCTGTTGTTTATGATCTCATGTCTGAGATGGCTTTTCAACAGAACAAAGTTGATGTTAAGGACTGGATTAATCTGTATTCAACGAGACGTTATGGTCGTGCAGTTCCTTTAGTGCAACGTGCCTGGAGTATATTACATCATACCATCTATAATTGCACTGATGGTGCCTACGACAAAAATAGGGATGTGATTGTGGCATTTCCGGACGTTGATCCATCCTTTATAGCAAGACCGTCTCAAGGTTATTACCAAAATGATAAATCATTAGTATCAAGAAGATCAGTCCTGAAGGAAATAACTGATTCATTTGAGCAACCTCATTTATGGTACTCGACTTCTGAAGTAGTACATGCATTAGAACTTTTTATTGCAAGTGGAGATGAACTCTCAGAAAGCAACACTTACAGGTATGACCTTGTTGACCTGACTAGACAAGCTTTGGCCAAGTATGCAAATCAGCTGTTTCTGAAGGTCATAGAAGCCTATCATTTAAATGATATCCTTGAAGTAGTTGGCCTCAGCCAGAAGTTTTTGGAACTGGTTGAAGACATGGACACACTCTTAGCATGCCATGATGGATTTCTTCTTGGACCATGGTTGGAAAGTTCAAAGAAAGTCGCCCAAGACAAAGAGCAGGAAATAAAGTTTGAATGGAATGCTAGAACTCAAATAACTATGTGGTTTGACAACACAGAAGAGGAAGCAAGTCTTCTTCGCGACTATGGAAACAAGTATTGGAGTGGGCTTCTGCGAGACTACTATGGTCCCCGAGCAGCCATATACTTCAAGTATCTGATTAAAAGCTTGGACGAGGGTAATGATTTTGACTTGAAGAACTGGAGGAGGGAGTGGATCAAGCTCACAAATGACTGGCAAAACAGCAGGAACACATTCCCGGTGAAAAGCACCGGAAATGCGGTGACTACTTCTCGGTTGCTTTTCGATAAGTACCTGCGAAGCGCTGACATAGAATCACTGGATGAAGCTGCTATTATGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
NAGLU NAGLU_C NAGLU_N

Protein Analysis

836

Amino Acids

95.44

Weight (kDa)

5.48

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAGLU_N PF12971 57 - 142 2.5e-20 Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain
NAGLU PF05089 169 - 515 3.9e-136 Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
NAGLU_C PF12972 524 - 818 1.2e-85 Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1599
Acc16I TGCGCA 1 cut(s) 420
Acc36I ACCTGC 2 cut(s) 956, 2466
AccB1I GGYRCC 1 cut(s) 1670
AccBSI CCGCTC 1 cut(s) 176
AccI GTMKAC 1 cut(s) 298
AccII CGCG 3 cut(s) 141, 180, 2223
AccIII TCCGGA 2 cut(s) 326, 1708
AciI CCGC 9 cut(s) 21, 139, 171, 174, 180, 190, 193, 758, 2423
AclI AACGTT 1 cut(s) 1392
AclWI GGATC 7 cut(s) 302, 315, 968, 1085, 1187, 1712, 2366
AcoI YGGCCR 1 cut(s) 1947
AcsI RAATTY 3 cut(s) 656, 730, 798
AcuI CTGAAG 6 cut(s) 116, 1284, 1296, 1814, 1866, 1994
AdeI CACNNNGTG 1 cut(s) 69
AfaI GTAC 6 cut(s) 186, 1100, 1403, 1835, 1854, 2456
AfeI AGCGCT 1 cut(s) 2467
AfiI CCNNNNNNNGG 4 cut(s) 145, 443, 1470, 1745
AjnI CCWGG 2 cut(s) 591, 1628
AjuI GAANNNNNNNTTGG 2 cut(s) 2224, 2256
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 8 cut(s) 219, 531, 781, 1943, 1966, 2310, 2365, 2492
AluI AGCT 8 cut(s) 219, 531, 781, 1943, 1966, 2310, 2365, 2492
Alw26I GTCTC 6 cut(s) 241, 481, 723, 1585, 1746, 2253
AlwI GGATC 7 cut(s) 302, 315, 968, 1085, 1187, 1712, 2366
AlwNI CAGNNNCTG 1 cut(s) 319
Ama87I CYCGRG 1 cut(s) 2274
Aor13HI TCCGGA 2 cut(s) 326, 1708
Aor51HI AGCGCT 1 cut(s) 2467
AoxI GGCC 6 cut(s) 147, 585, 697, 1205, 1947, 2020
ApeKI GCWGC 4 cut(s) 866, 1372, 2279, 2492
ApoI RAATTY 3 cut(s) 656, 730, 798
AseI ATTAAT 1 cut(s) 1575
Asp700I GAANNNNTTC 3 cut(s) 647, 1001, 2396
AspLEI GCGC 2 cut(s) 421, 2468
AsuC2I CCSGG 1 cut(s) 2402
AsuHPI GGTGA 3 cut(s) 55, 2416, 2437
AvaI CYCGRG 1 cut(s) 2274
AvaII GGWCC 7 cut(s) 136, 749, 945, 1035, 1430, 2096, 2270
BalI TGGCCA 1 cut(s) 1949
BamHI GGATCC 1 cut(s) 307
BanI GGYRCC 1 cut(s) 1670
BauI CACGAG 1 cut(s) 456
BbsI GAAGAC 2 cut(s) 2058, 2207
BbvCI CCTCAGC 1 cut(s) 2024
BbvI GCAGC 4 cut(s) 878, 1359, 2291, 2479
BceAI ACGGC 1 cut(s) 1420
BciT130I CCWGG 2 cut(s) 593, 1630
BclI TGATCA 1 cut(s) 772
BcnI CCSGG 1 cut(s) 2402
BcoDI GTCTC 6 cut(s) 241, 481, 723, 1585, 1746, 2253
BfaI CTAG 5 cut(s) 528, 611, 939, 1935, 2166
BfmI CTRYAG 3 cut(s) 788, 864, 1242
BfoI RGCGCY 1 cut(s) 2469
BfuAI ACCTGC 2 cut(s) 956, 2466
BisI GCNGC 7 cut(s) 171, 174, 193, 867, 1373, 2280, 2493
BlsI GCNGC 7 cut(s) 172, 175, 194, 868, 1374, 2281, 2494
BmcAI AGTACT 1 cut(s) 186
Bme1390I CCNGG 3 cut(s) 593, 1630, 2402
Bme18I GGWCC 7 cut(s) 136, 749, 945, 1035, 1430, 2096, 2270
BmeT110I CYCGRG 1 cut(s) 2274
BmiI GGNNCC 4 cut(s) 6, 309, 1672, 2272
BmrFI CCNGG 3 cut(s) 593, 1630, 2402
BmsI GCATC 5 cut(s) 269, 955, 1405, 1429, 1433
BoxI GACNNNNGTC 1 cut(s) 833
BpiI GAAGAC 2 cut(s) 2058, 2207
BplI GAGNNNNNCTC 8 cut(s) 134, 166, 136, 168, 1088, 1120, 1875, 1907
BpmI CTGGAG 7 cut(s) 199, 357, 575, 816, 1222, 1651, 2365
Bpu10I CCTNAGC 1 cut(s) 2024
BpuEI CTTGAG 1 cut(s) 1728
BpuMI CCSGG 1 cut(s) 2402
BsaI GGTCTC 2 cut(s) 481, 723
BsaJI CCNNGG 5 cut(s) 497, 592, 820, 1464, 2099
BsaWI WCCGGW 3 cut(s) 326, 1708, 2414
Bsc4I CCNNNNNNNGG 4 cut(s) 145, 443, 1470, 1745
Bse3DI GCAATG 1 cut(s) 254
BseAI TCCGGA 2 cut(s) 326, 1708
BseBI CCWGG 2 cut(s) 593, 1630
BseDI CCNNGG 5 cut(s) 497, 592, 820, 1464, 2099
BseLI CCNNNNNNNGG 4 cut(s) 145, 443, 1470, 1745
BseMI GCAATG 1 cut(s) 254
BseMII CTCAG 3 cut(s) 1521, 1908, 2038
BseRI GAGGAG 2 cut(s) 70, 2362
BseXI GCAGC 4 cut(s) 878, 1359, 2291, 2479
BsgI GTGCAG 4 cut(s) 934, 1194, 1358, 1626
Bsh1236I CGCG 3 cut(s) 141, 180, 2223
BshFI GGCC 6 cut(s) 149, 587, 699, 1207, 1949, 2022
BshNI GGYRCC 1 cut(s) 1670
BsiHKCI CYCGRG 1 cut(s) 2274
BsiSI CCGG 4 cut(s) 327, 1709, 2402, 2415
BslFI GGGAC 4 cut(s) 419, 563, 1146, 2256
BslI CCNNNNNNNGG 4 cut(s) 145, 443, 1470, 1745
BsmAI GTCTC 6 cut(s) 241, 481, 723, 1585, 1746, 2253
BsmBI CGTCTC 3 cut(s) 241, 1585, 1746
BsmFI GGGAC 4 cut(s) 419, 563, 1146, 2256
BsmI GAATGC 2 cut(s) 520, 2167
BsnI GGCC 6 cut(s) 149, 587, 699, 1207, 1949, 2022
Bso31I GGTCTC 2 cut(s) 481, 723
BsoBI CYCGRG 1 cut(s) 2274
Bsp13I TCCGGA 2 cut(s) 326, 1708
Bsp19I CCATGG 2 cut(s) 1464, 2099
Bsp68I TCGCGA 1 cut(s) 2223
BspACI CCGC 9 cut(s) 21, 139, 171, 174, 180, 190, 193, 758, 2423
BspANI GGCC 6 cut(s) 149, 587, 699, 1207, 1949, 2022
BspCNI CTCAG 3 cut(s) 1522, 1907, 2037
BspEI TCCGGA 2 cut(s) 326, 1708
BspFNI CGCG 3 cut(s) 141, 180, 2223
BspLI GGNNCC 4 cut(s) 6, 309, 1672, 2272
BspMAI CTGCAG 3 cut(s) 792, 868, 1246
BspMI ACCTGC 2 cut(s) 956, 2466
BspPI GGATC 7 cut(s) 302, 315, 968, 1085, 1187, 1712, 2366
BspQI GCTCTTC 1 cut(s) 56
BspT107I GGYRCC 1 cut(s) 1670
BspTNI GGTCTC 2 cut(s) 481, 723
BsrBI CCGCTC 1 cut(s) 176
BsrDI GCAATG 1 cut(s) 254
BssECI CCNNGG 5 cut(s) 497, 592, 820, 1464, 2099
BssSI CACGAG 1 cut(s) 456
BssT1I CCWWGG 4 cut(s) 497, 820, 1464, 2099
Bst2BI CACGAG 1 cut(s) 456
Bst2UI CCWGG 2 cut(s) 593, 1630
Bst4CI ACNGT 4 cut(s) 906, 1055, 1157, 1740
Bst6I CTCTTC 2 cut(s) 56, 2196
BstC8I GCNNGC 3 cut(s) 178, 1209, 2074
BstDEI CTNAG 5 cut(s) 240, 1530, 1894, 2024, 2068
BstDSI CCRYGG 2 cut(s) 1464, 2099
BstFNI CGCG 3 cut(s) 141, 180, 2223
BstH2I RGCGCY 1 cut(s) 2469
BstHHI GCGC 2 cut(s) 421, 2468
BstMAI GTCTC 6 cut(s) 241, 481, 723, 1585, 1746, 2253
BstMWI GCNNNNNNNGC 4 cut(s) 710, 766, 787, 1955
BstNI CCWGG 2 cut(s) 593, 1630
BstNSI RCATGY 2 cut(s) 1859, 2076
BstPAI GACNNNNGTC 1 cut(s) 833
BstSCI CCNGG 3 cut(s) 591, 1628, 2400
BstSFI CTRYAG 3 cut(s) 788, 864, 1242
BstUI CGCG 3 cut(s) 141, 180, 2223
BstV1I GCAGC 4 cut(s) 878, 1359, 2291, 2479
BstV2I GAAGAC 2 cut(s) 2058, 2207
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BsuRI GGCC 6 cut(s) 149, 587, 699, 1207, 1949, 2022
BtgI CCRYGG 2 cut(s) 1464, 2099
BtsIMutI CAGTG 3 cut(s) 333, 1662, 2480
BtuMI TCGCGA 1 cut(s) 2223
BveI ACCTGC 2 cut(s) 956, 2466
Cac8I GCNNGC 3 cut(s) 178, 1209, 2074
CaiI CAGNNNCTG 1 cut(s) 319
CfoI GCGC 2 cut(s) 421, 2468
Csp6I GTAC 6 cut(s) 185, 1099, 1402, 1834, 1853, 2455
CviAII CATG 9 cut(s) 714, 737, 1465, 1525, 1856, 2056, 2073, 2078, 2100
CviQI GTAC 6 cut(s) 185, 1099, 1402, 1834, 1853, 2455
DdeI CTNAG 5 cut(s) 240, 1530, 1894, 2024, 2068
DraI TTTAAA 1 cut(s) 1998
DraIII CACNNNGTG 1 cut(s) 69
DrdI GACNNNNNNGTC 1 cut(s) 1599
DseDI GACNNNNNNGTC 1 cut(s) 1599
EaeI YGGCCR 1 cut(s) 1947
Eam1104I CTCTTC 2 cut(s) 56, 2196
EarI CTCTTC 2 cut(s) 56, 2196
EciI GGCGGA 1 cut(s) 179
Eco130I CCWWGG 4 cut(s) 497, 820, 1464, 2099
Eco147I AGGCCT 1 cut(s) 1207
Eco31I GGTCTC 2 cut(s) 481, 723
Eco32I GATATC 1 cut(s) 2005
Eco47I GGWCC 7 cut(s) 136, 749, 945, 1035, 1430, 2096, 2270
Eco47III AGCGCT 1 cut(s) 2467
Eco57I CTGAAG 6 cut(s) 116, 1284, 1296, 1814, 1866, 1994
Eco88I CYCGRG 1 cut(s) 2274
EcoO109I RGGNCCY 2 cut(s) 147, 698
EcoRII CCWGG 2 cut(s) 591, 1628
EcoRV GATATC 1 cut(s) 2005
EcoT14I CCWWGG 4 cut(s) 497, 820, 1464, 2099
EcoT22I ATGCAT 1 cut(s) 1861
ErhI CCWWGG 4 cut(s) 497, 820, 1464, 2099
Esp3I CGTCTC 3 cut(s) 241, 1585, 1746
FaeI CATG 9 cut(s) 717, 740, 1468, 1528, 1859, 2059, 2076, 2081, 2103
FalI AAGNNNNNCTT 6 cut(s) 1211, 1243, 1891, 1923, 2199, 2231
FaqI GGGAC 4 cut(s) 419, 563, 1146, 2256
FatI CATG 9 cut(s) 713, 736, 1464, 1524, 1855, 2055, 2072, 2077, 2099
FauI CCCGC 2 cut(s) 28, 765
FauNDI CATATG 1 cut(s) 1189
FbaI TGATCA 1 cut(s) 772
FblI GTMKAC 1 cut(s) 298
Fnu4HI GCNGC 7 cut(s) 171, 174, 193, 867, 1373, 2280, 2493
Fsp4HI GCNGC 7 cut(s) 171, 174, 193, 867, 1373, 2280, 2493
FspBI CTAG 5 cut(s) 528, 611, 939, 1935, 2166
FspI TGCGCA 1 cut(s) 420
GlaI GCGC 2 cut(s) 420, 2467
GluI GCNGC 7 cut(s) 171, 174, 193, 867, 1373, 2280, 2493
GsuI CTGGAG 7 cut(s) 199, 357, 575, 816, 1222, 1651, 2365
HaeII RGCGCY 1 cut(s) 2469
HaeIII GGCC 6 cut(s) 149, 587, 699, 1207, 1949, 2022
HapII CCGG 4 cut(s) 327, 1709, 2402, 2415
HhaI GCGC 2 cut(s) 421, 2468
Hin1II CATG 9 cut(s) 717, 740, 1468, 1528, 1859, 2059, 2076, 2081, 2103
Hin6I GCGC 2 cut(s) 419, 2466
HinP1I GCGC 2 cut(s) 419, 2466
HincII GTYRAC 1 cut(s) 1927
HindII GTYRAC 1 cut(s) 1927
HindIII AAGCTT 2 cut(s) 1941, 2308
HinfI GANTC 7 cut(s) 102, 316, 330, 784, 829, 1808, 2477
HpaII CCGG 4 cut(s) 327, 1709, 2402, 2415
HphI GGTGA 3 cut(s) 55, 2416, 2437
Hpy166II GTNNAC 3 cut(s) 299, 749, 1927
Hpy188I TCNGA 9 cut(s) 101, 321, 676, 1303, 1531, 1846, 1897, 1974, 2301
Hpy8I GTNNAC 3 cut(s) 299, 749, 1927
HpyAV CCTTC 9 cut(s) 213, 297, 848, 968, 1026, 1213, 1484, 1789, 1969
HpyCH4III ACNGT 4 cut(s) 906, 1055, 1157, 1740
HpyCH4IV ACGT 4 cut(s) 1392, 1594, 1624, 1713
HpyF10VI GCNNNNNNNGC 4 cut(s) 710, 766, 787, 1955
HpyF3I CTNAG 5 cut(s) 240, 1530, 1894, 2024, 2068
HpySE526I ACGT 4 cut(s) 1392, 1594, 1624, 1713
Hsp92II CATG 9 cut(s) 717, 740, 1468, 1528, 1859, 2059, 2076, 2081, 2103
HspAI GCGC 2 cut(s) 419, 2466
Kpn2I TCCGGA 2 cut(s) 326, 1708
Ksp22I TGATCA 1 cut(s) 772
LguI GCTCTTC 1 cut(s) 56
LmnI GCTCC 2 cut(s) 10, 244
Lsp1109I GCAGC 4 cut(s) 878, 1359, 2291, 2479
LweI GCATC 5 cut(s) 269, 955, 1405, 1429, 1433
MaeI CTAG 5 cut(s) 528, 611, 939, 1935, 2166
MaeII ACGT 4 cut(s) 1392, 1594, 1624, 1713
MaeIII GTNAC 5 cut(s) 331, 520, 932, 1040, 2425
MbiI CCGCTC 1 cut(s) 176
MfeI CAATTG 1 cut(s) 1434
MflI RGATCY 1 cut(s) 307
MlsI TGGCCA 1 cut(s) 1949
MluCI AATT 7 cut(s) 376, 656, 730, 798, 990, 1434, 1657
MluNI TGGCCA 1 cut(s) 1949
MlyI GAGTC 3 cut(s) 111, 339, 823
MmeI TCCRAC 2 cut(s) 1456, 2085
Mox20I TGGCCA 1 cut(s) 1949
Mph1103I ATGCAT 1 cut(s) 1861
MroI TCCGGA 2 cut(s) 326, 1708
MroXI GAANNNNTTC 3 cut(s) 647, 1001, 2396
MscI TGGCCA 1 cut(s) 1949
MseI TTAA 8 cut(s) 276, 660, 927, 1128, 1563, 1575, 1997, 2304
MslI CAYNNNNRTG 2 cut(s) 338, 1998
Msp20I TGGCCA 1 cut(s) 1949
MspA1I CMGCKG 2 cut(s) 781, 1966
MspI CCGG 4 cut(s) 327, 1709, 2402, 2415
MspR9I CCNGG 3 cut(s) 593, 1630, 2402
MunI CAATTG 1 cut(s) 1434
Mva1269I GAATGC 2 cut(s) 520, 2167
MvaI CCWGG 2 cut(s) 593, 1630
MvnI CGCG 3 cut(s) 141, 180, 2223
MwoI GCNNNNNNNGC 4 cut(s) 710, 766, 787, 1955
NciI CCSGG 1 cut(s) 2402
NcoI CCATGG 2 cut(s) 1464, 2099
NdeI CATATG 1 cut(s) 1189
NlaIII CATG 9 cut(s) 717, 740, 1468, 1528, 1859, 2059, 2076, 2081, 2103
NlaIV GGNNCC 4 cut(s) 6, 309, 1672, 2272
NmuCI GTSAC 5 cut(s) 331, 520, 932, 1040, 2425
NruI TCGCGA 1 cut(s) 2223
NsbI TGCGCA 1 cut(s) 420
NsiI ATGCAT 1 cut(s) 1861
NspI RCATGY 2 cut(s) 1859, 2076
OliI CACNNNNGTG 1 cut(s) 338
PaeI GCATGC 1 cut(s) 2076
PceI AGGCCT 1 cut(s) 1207
PciSI GCTCTTC 1 cut(s) 56
PcsI WCGNNNNNNNCGW 1 cut(s) 1600
PctI GAATGC 2 cut(s) 520, 2167
PdmI GAANNNNTTC 3 cut(s) 647, 1001, 2396
PfeI GAWTC 4 cut(s) 316, 784, 1808, 2477
PkrI GCNGC 7 cut(s) 172, 175, 194, 868, 1374, 2281, 2494
PleI GAGTC 3 cut(s) 110, 338, 823
PpsI GAGTC 3 cut(s) 110, 338, 823
PshAI GACNNNNGTC 1 cut(s) 833
PshBI ATTAAT 1 cut(s) 1575
Psp1406I AACGTT 1 cut(s) 1392
Psp6I CCWGG 2 cut(s) 591, 1628
PspGI CCWGG 2 cut(s) 591, 1628
PspN4I GGNNCC 4 cut(s) 6, 309, 1672, 2272
PstI CTGCAG 3 cut(s) 792, 868, 1246
PstNI CAGNNNCTG 1 cut(s) 319
PsuI RGATCY 1 cut(s) 307
PvuII CAGCTG 2 cut(s) 781, 1966
RruI TCGCGA 1 cut(s) 2223
RsaI GTAC 6 cut(s) 186, 1100, 1403, 1835, 1854, 2456
RsaNI GTAC 6 cut(s) 185, 1099, 1402, 1834, 1853, 2455
RseI CAYNNNNRTG 2 cut(s) 338, 1998
SapI GCTCTTC 1 cut(s) 56
SaqAI TTAA 8 cut(s) 276, 660, 927, 1128, 1563, 1575, 1997, 2304
SatI GCNGC 7 cut(s) 171, 174, 193, 867, 1373, 2280, 2493
SbfI CCTGCAGG 1 cut(s) 1246
ScaI AGTACT 1 cut(s) 186
SchI GAGTC 3 cut(s) 111, 339, 823
ScrFI CCNGG 3 cut(s) 593, 1630, 2402
SdaI CCTGCAGG 1 cut(s) 1246
SfaNI GCATC 5 cut(s) 269, 955, 1405, 1429, 1433
SfcI CTRYAG 3 cut(s) 788, 864, 1242
SinI GGWCC 7 cut(s) 136, 749, 945, 1035, 1430, 2096, 2270
SmiI ATTTAAAT 1 cut(s) 1998
SmiMI CAYNNNNRTG 2 cut(s) 338, 1998
SmlI CTYRAG 1 cut(s) 1743
SmoI CTYRAG 1 cut(s) 1743
SphI GCATGC 1 cut(s) 2076
Sse8387I CCTGCAGG 1 cut(s) 1246
Sse9I AATT 7 cut(s) 376, 656, 730, 798, 990, 1434, 1657
SseBI AGGCCT 1 cut(s) 1207
SsiI CCGC 9 cut(s) 21, 139, 171, 174, 180, 190, 193, 758, 2423
SspI AATATT 1 cut(s) 1125
SspMI CTAG 5 cut(s) 528, 611, 939, 1935, 2166
StuI AGGCCT 1 cut(s) 1207
StyD4I CCNGG 3 cut(s) 591, 1628, 2400
StyI CCWWGG 4 cut(s) 497, 820, 1464, 2099
SwaI ATTTAAAT 1 cut(s) 1998
TaaI ACNGT 4 cut(s) 906, 1055, 1157, 1740
TaiI ACGT 4 cut(s) 1395, 1597, 1627, 1716
TaqI TCGA 5 cut(s) 116, 222, 721, 1838, 2448
TasI AATT 7 cut(s) 376, 656, 730, 798, 990, 1434, 1657
TatI WGTACW 3 cut(s) 184, 1098, 1852
TauI GCSGC 3 cut(s) 173, 176, 195
TfiI GAWTC 4 cut(s) 316, 784, 1808, 2477
Tru1I TTAA 8 cut(s) 276, 660, 927, 1128, 1563, 1575, 1997, 2304
Tru9I TTAA 8 cut(s) 276, 660, 927, 1128, 1563, 1575, 1997, 2304
TscAI CASTG 3 cut(s) 340, 1669, 2487
TseFI GTSAC 5 cut(s) 331, 520, 932, 1040, 2425
TseI GCWGC 4 cut(s) 866, 1372, 2279, 2492
Tsp45I GTSAC 5 cut(s) 331, 520, 932, 1040, 2425
TspDTI ATGAA 7 cut(s) 994, 1176, 1232, 1346, 1800, 1902, 2502
TspRI CASTG 3 cut(s) 340, 1669, 2487
VpaK11BI GGWCC 7 cut(s) 136, 749, 945, 1035, 1430, 2096, 2270
VspI ATTAAT 1 cut(s) 1575
XapI RAATTY 3 cut(s) 656, 730, 798
XceI RCATGY 2 cut(s) 1859, 2076
XcmI CCANNNNNNNNNTGG 1 cut(s) 1440
XmiI GTMKAC 1 cut(s) 298
XmnI GAANNNNTTC 3 cut(s) 647, 1001, 2396
XspI CTAG 5 cut(s) 528, 611, 939, 1935, 2166
ZrmI AGTACT 1 cut(s) 186
Zsp2I ATGCAT 1 cut(s) 1861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.