pycom14g15500

alpha-N-acetylglucosaminidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0000933
Physical Location & Seq
Forward (+)
18121714 .. 18124734
3021 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g15500.6

Sequence Viewer

Length: 1356 bp
ATGGCCTCCAAATTTCCCACCTTTTCGCCGGCCTTCACAATCCTCCTTACTATCCCCTTCCTCGACTTGGCTTACTCGTCCACCGTCGGTGTCCGACACATTTCGCGCCTTCTCGAAATCCAGGACCGCGAGAAGGCGCCGCCGTACGTTCAAGTCGCTGCCGCTCGCGGAGTCCTCCGCCGCCTTCTCCCCTCTCACTCTTCCAGCTTCGACTTCCAAATCGTCTCCAAGGAGCAATGTGGGGGTGCATCTTGCTTTATGATCGATCACAATCCTTCTTTTCGCAGACGCGGGGATCCTCGAATTCTAATTTCTGGGGTCACTGGAGTGGAGATTTTGGCTGGTCTGCACTGGTACTTGAAGAATTTGTGTAATTCACATATATCTTGGGATAAAACTGGTGGTGCGCAACTATTTTCAGTCCCGAAATCAGGGTTGCTCACCCCGGTGCAAGATGCCGGCATTTTGGTCCAGAGACCTGTTCCTTGGAACTACTACCAGAATGCTGTTACTTCCAGCTATACATTCGCCTGGTGGGATTGGGAAAGATGGGAGAAGGAGATTGACTGGATGGCCCTTCAAGGGATCAATTTGCCACTAGCATTTACAGGGCAAGAGGCTATTTGGCAGAAAGTTTTCCAGAAATTTAATATGAGCAAATCAGAACTGGATGAATTTTTTGGAGGCCCCGCTTTCCTTGCTTGGTCTCGTATGGGAAATTTGCATGGATGGGGCGGACCTCTACCTCAAAGTTGGGTGGATCAACAGCTGATTCTGCAGAAAAAGATTCTGGTCAGAATGTATGAACTTGGAATGACTCCAGTCCTTCCAGCCTTTTCCGGAAATGTTCCTGCAGCGCTCAAAACTACATATCCATCAGCGAAGATAACACGCTTAGGAAATTGGTTTTCTGTTAAGAGTGATCCTAGATGGACCTGCACTTACCTTCTTGATGCAACTGATCCGTTGTTTGTTGAAATTGGGAGAGCATTCATTGATCAACAAGTAAAAGAGTACGGCAGGACTAGTCACATATACAACTGTGATACTTTTGATGAGAATACTCCACCTGACGATGCTCCGGAGTACATATCTTCGTTAGGTGCAGCAATCTTTAGGGGTATGCAAAGTGGAGACAAGGACGGTGTCTGGTTAATGCAGGGGTGGTTATTTTCATACGATCCATTCTGGAGGCCTCCGCAAATGAAGGCACTTTTACACTCTGTTCCCGCGGGGAAGCTGGTAGTACTTGATCTGTTTGCTGAAGTGAAACCGATATGGAGTACTACGGAGCAGTTTTATGGTGTTCCTTACATCTGGAAAGTGTATGCTGCACAATTTTGCAGGAAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
NAGLU NAGLU_C NAGLU_N

Protein Analysis

452

Amino Acids

51.18

Weight (kDa)

8.34

Isoelectric Point (pI)

49.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 408
Acc36I ACCTGC 1 cut(s) 944
AccB1I GGYRCC 1 cut(s) 136
AccBSI CCGCTC 1 cut(s) 164
AccII CGCG 5 cut(s) 106, 129, 168, 291, 1232
AccIII TCCGGA 2 cut(s) 839, 1081
AclWI GGATC 7 cut(s) 290, 303, 593, 768, 917, 956, 1175
AcsI RAATTY 6 cut(s) 11, 303, 364, 644, 674, 718
AcuI CTGAAG 1 cut(s) 1284
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 6 cut(s) 146, 356, 1016, 1088, 1248, 1285
AfeI AGCGCT 1 cut(s) 858
AfiI CCNNNNNNNGG 4 cut(s) 67, 133, 431, 582
AgsI TTSAA 4 cut(s) 152, 361, 581, 977
AhlI ACTAGT 1 cut(s) 1025
AjnI CCWGG 2 cut(s) 120, 530
AleI CACNNNNGTG 2 cut(s) 326, 446
AluBI AGCT 4 cut(s) 207, 519, 769, 1240
AluI AGCT 4 cut(s) 207, 519, 769, 1240
Alw26I GTCTC 4 cut(s) 229, 469, 711, 1128
AlwI GGATC 7 cut(s) 290, 303, 593, 768, 917, 956, 1175
Aor13HI TCCGGA 2 cut(s) 839, 1081
Aor51HI AGCGCT 1 cut(s) 858
AoxI GGCC 5 cut(s) 3, 30, 573, 685, 1193
ApeKI GCWGC 4 cut(s) 158, 854, 1106, 1331
ApoI RAATTY 6 cut(s) 11, 303, 364, 644, 674, 718
Asp700I GAANNNNTTC 1 cut(s) 635
AspLEI GCGC 4 cut(s) 108, 139, 409, 859
AspS9I GGNCC 6 cut(s) 124, 469, 574, 686, 737, 933
AsuC2I CCSGG 1 cut(s) 446
AsuHPI GGTGA 1 cut(s) 433
AvaII GGWCC 4 cut(s) 124, 469, 737, 933
BamHI GGATCC 1 cut(s) 295
BanI GGYRCC 1 cut(s) 136
BbvI GCAGC 4 cut(s) 145, 866, 1118, 1318
BccI CCATC 5 cut(s) 543, 565, 723, 883, 924
BceAI ACGGC 2 cut(s) 127, 1033
BciT130I CCWGG 2 cut(s) 122, 532
BclI TGATCA 1 cut(s) 997
BcnI CCSGG 1 cut(s) 446
BcoDI GTCTC 4 cut(s) 229, 469, 711, 1128
BcuI ACTAGT 1 cut(s) 1025
BfaI CTAG 3 cut(s) 599, 927, 1026
BfmI CTRYAG 2 cut(s) 776, 852
BfoI RGCGCY 2 cut(s) 140, 860
BfuAI ACCTGC 1 cut(s) 944
BisI GCNGC 7 cut(s) 140, 159, 162, 181, 855, 1107, 1332
BlsI GCNGC 7 cut(s) 141, 160, 163, 182, 856, 1108, 1333
BmcAI AGTACT 2 cut(s) 1248, 1285
Bme1390I CCNGG 3 cut(s) 122, 446, 532
Bme18I GGWCC 4 cut(s) 124, 469, 737, 933
BmgT120I GGNCC 6 cut(s) 124, 469, 574, 686, 737, 933
BmiI GGNNCC 3 cut(s) 138, 297, 688
BmrFI CCNGG 3 cut(s) 122, 446, 532
BmsI GCATC 4 cut(s) 257, 445, 943, 1066
BoxI GACNNNNGTC 1 cut(s) 821
BpmI CTGGAG 3 cut(s) 345, 804, 1210
Bpu10I CCTNAGC 1 cut(s) 895
BpuMI CCSGG 1 cut(s) 446
Bsa29I ATCGAT 1 cut(s) 264
BsaBI GATNNNNATC 1 cut(s) 270
BsaHI GRCGYC 1 cut(s) 137
BsaI GGTCTC 2 cut(s) 469, 711
BsaJI CCNNGG 4 cut(s) 228, 444, 485, 1230
BsaWI WCCGGW 2 cut(s) 839, 1081
Bsc4I CCNNNNNNNGG 4 cut(s) 67, 133, 431, 582
Bse118I RCCGGY 2 cut(s) 28, 458
Bse1I ACTGG 6 cut(s) 328, 356, 403, 572, 672, 821
Bse3DI GCAATG 1 cut(s) 242
Bse8I GATNNNNATC 1 cut(s) 270
BseAI TCCGGA 2 cut(s) 839, 1081
BseBI CCWGG 2 cut(s) 122, 532
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 4 cut(s) 228, 444, 485, 1230
BseGI GGATG 3 cut(s) 576, 676, 734
BseJI GATNNNNATC 1 cut(s) 270
BseLI CCNNNNNNNGG 4 cut(s) 67, 133, 431, 582
BseMI GCAATG 1 cut(s) 242
BseNI ACTGG 6 cut(s) 328, 356, 403, 572, 672, 821
BseXI GCAGC 4 cut(s) 145, 866, 1118, 1318
BsgI GTGCAG 4 cut(s) 332, 922, 1125, 1317
Bsh1236I CGCG 5 cut(s) 106, 129, 168, 291, 1232
BshFI GGCC 5 cut(s) 5, 32, 575, 687, 1195
BshNI GGYRCC 1 cut(s) 136
BshVI ATCGAT 1 cut(s) 264
BsiSI CCGG 5 cut(s) 29, 446, 459, 840, 1082
BsiWI CGTACG 1 cut(s) 144
BslFI GGGAC 1 cut(s) 407
BslI CCNNNNNNNGG 4 cut(s) 67, 133, 431, 582
BsmAI GTCTC 4 cut(s) 229, 469, 711, 1128
BsmBI CGTCTC 1 cut(s) 229
BsmFI GGGAC 1 cut(s) 407
BsmI GAATGC 2 cut(s) 508, 989
BsnI GGCC 5 cut(s) 5, 32, 575, 687, 1195
Bso31I GGTCTC 2 cut(s) 469, 711
Bsp13I TCCGGA 2 cut(s) 839, 1081
BspANI GGCC 5 cut(s) 5, 32, 575, 687, 1195
BspDI ATCGAT 1 cut(s) 264
BspEI TCCGGA 2 cut(s) 839, 1081
BspFNI CGCG 5 cut(s) 106, 129, 168, 291, 1232
BspLI GGNNCC 3 cut(s) 138, 297, 688
BspMAI CTGCAG 2 cut(s) 780, 856
BspMI ACCTGC 1 cut(s) 944
BspPI GGATC 7 cut(s) 290, 303, 593, 768, 917, 956, 1175
BspT107I GGYRCC 1 cut(s) 136
BspTNI GGTCTC 2 cut(s) 469, 711
BsrBI CCGCTC 1 cut(s) 164
BsrDI GCAATG 1 cut(s) 242
BsrFI RCCGGY 2 cut(s) 28, 458
BsrI ACTGG 6 cut(s) 328, 356, 403, 572, 672, 821
BssAI RCCGGY 2 cut(s) 28, 458
BssECI CCNNGG 4 cut(s) 228, 444, 485, 1230
BssNI GRCGYC 1 cut(s) 137
BssT1I CCWWGG 2 cut(s) 228, 485
Bst2UI CCWGG 2 cut(s) 122, 532
Bst4CI ACNGT 3 cut(s) 85, 1043, 1145
Bst6I CTCTTC 1 cut(s) 205
BstACI GRCGYC 1 cut(s) 137
BstC8I GCNNGC 3 cut(s) 30, 166, 460
BstDEI CTNAG 1 cut(s) 895
BstDSI CCRYGG 1 cut(s) 1230
BstF5I GGATG 3 cut(s) 576, 676, 734
BstFNI CGCG 5 cut(s) 106, 129, 168, 291, 1232
BstH2I RGCGCY 2 cut(s) 140, 860
BstHHI GCGC 4 cut(s) 108, 139, 409, 859
BstMAI GTCTC 4 cut(s) 229, 469, 711, 1128
BstMWI GCNNNNNNNGC 2 cut(s) 698, 775
BstNI CCWGG 2 cut(s) 122, 532
BstPAI GACNNNNGTC 1 cut(s) 821
BstSCI CCNGG 3 cut(s) 120, 444, 530
BstSFI CTRYAG 2 cut(s) 776, 852
BstUI CGCG 5 cut(s) 106, 129, 168, 291, 1232
BstV1I GCAGC 4 cut(s) 145, 866, 1118, 1318
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
Bsu15I ATCGAT 1 cut(s) 264
BsuRI GGCC 5 cut(s) 5, 32, 575, 687, 1195
BsuTUI ATCGAT 1 cut(s) 264
BtgI CCRYGG 1 cut(s) 1230
BtsCI GGATG 3 cut(s) 576, 676, 734
BtsIMutI CAGTG 2 cut(s) 321, 349
BveI ACCTGC 1 cut(s) 944
Cac8I GCNNGC 3 cut(s) 30, 166, 460
CfoI GCGC 4 cut(s) 108, 139, 409, 859
Cfr10I RCCGGY 2 cut(s) 28, 458
Cfr13I GGNCC 6 cut(s) 124, 469, 574, 686, 737, 933
Cfr42I CCGCGG 1 cut(s) 1233
ClaI ATCGAT 1 cut(s) 264
CseI GACGC 1 cut(s) 297
Csp6I GTAC 6 cut(s) 145, 355, 1015, 1087, 1247, 1284
CviAII CATG 1 cut(s) 725
CviQI GTAC 6 cut(s) 145, 355, 1015, 1087, 1247, 1284
DdeI CTNAG 1 cut(s) 895
DinI GGCGCC 1 cut(s) 138
Eam1104I CTCTTC 1 cut(s) 205
EarI CTCTTC 1 cut(s) 205
EciI GGCGGA 2 cut(s) 167, 750
Eco130I CCWWGG 2 cut(s) 228, 485
Eco147I AGGCCT 1 cut(s) 1195
Eco31I GGTCTC 2 cut(s) 469, 711
Eco47I GGWCC 4 cut(s) 124, 469, 737, 933
Eco47III AGCGCT 1 cut(s) 858
Eco57I CTGAAG 1 cut(s) 1284
EcoO109I RGGNCCY 1 cut(s) 686
EcoRI GAATTC 1 cut(s) 303
EcoRII CCWGG 2 cut(s) 120, 530
EcoT14I CCWWGG 2 cut(s) 228, 485
EgeI GGCGCC 1 cut(s) 138
EheI GGCGCC 1 cut(s) 138
ErhI CCWWGG 2 cut(s) 228, 485
Esp3I CGTCTC 1 cut(s) 229
FaeI CATG 1 cut(s) 728
FaqI GGGAC 1 cut(s) 407
FatI CATG 1 cut(s) 724
FauI CCCGC 4 cut(s) 284, 697, 1225, 1237
FbaI TGATCA 1 cut(s) 997
Fnu4HI GCNGC 7 cut(s) 140, 159, 162, 181, 855, 1107, 1332
FokI GGATG 3 cut(s) 583, 683, 741
Fsp4HI GCNGC 7 cut(s) 140, 159, 162, 181, 855, 1107, 1332
FspBI CTAG 3 cut(s) 599, 927, 1026
FspI TGCGCA 1 cut(s) 408
GlaI GCGC 4 cut(s) 107, 138, 408, 858
GluI GCNGC 7 cut(s) 140, 159, 162, 181, 855, 1107, 1332
GsuI CTGGAG 3 cut(s) 345, 804, 1210
HaeII RGCGCY 2 cut(s) 140, 860
HaeIII GGCC 5 cut(s) 5, 32, 575, 687, 1195
HapII CCGG 5 cut(s) 29, 446, 459, 840, 1082
HgaI GACGC 1 cut(s) 297
HhaI GCGC 4 cut(s) 108, 139, 409, 859
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 1 cut(s) 728
Hin6I GCGC 4 cut(s) 106, 137, 407, 857
HinP1I GCGC 4 cut(s) 106, 137, 407, 857
HinfI GANTC 4 cut(s) 171, 772, 787, 817
HpaII CCGG 5 cut(s) 29, 446, 459, 840, 1082
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 1 cut(s) 81
Hpy188I TCNGA 3 cut(s) 95, 664, 797
Hpy188III TCNNGA 9 cut(s) 113, 424, 472, 640, 840, 950, 1082, 1189, 1318
Hpy8I GTNNAC 1 cut(s) 81
Hpy99I CGWCG 1 cut(s) 89
HpyCH4III ACNGT 3 cut(s) 85, 1043, 1145
HpyCH4IV ACGT 1 cut(s) 147
HpyF10VI GCNNNNNNNGC 2 cut(s) 698, 775
HpyF3I CTNAG 1 cut(s) 895
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 1 cut(s) 728
HspAI GCGC 4 cut(s) 106, 137, 407, 857
KasI GGCGCC 1 cut(s) 136
Kpn2I TCCGGA 2 cut(s) 839, 1081
KroI GCCGGC 2 cut(s) 28, 458
KroNI GCCGGC 2 cut(s) 30, 460
Ksp22I TGATCA 1 cut(s) 997
KspI CCGCGG 1 cut(s) 1233
LmnI GCTCC 3 cut(s) 232, 1084, 1291
Lsp1109I GCAGC 4 cut(s) 145, 866, 1118, 1318
LweI GCATC 4 cut(s) 257, 445, 943, 1066
MaeI CTAG 3 cut(s) 599, 927, 1026
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 3 cut(s) 319, 508, 1028
MbiI CCGCTC 1 cut(s) 164
MboII GAAGA 4 cut(s) 192, 373, 895, 1086
MflI RGATCY 1 cut(s) 295
Mly113I GGCGCC 1 cut(s) 137
MlyI GAGTC 2 cut(s) 180, 811
MmeI TCCRAC 1 cut(s) 118
MroI TCCGGA 2 cut(s) 839, 1081
MroNI GCCGGC 2 cut(s) 28, 458
MroXI GAANNNNTTC 1 cut(s) 635
MseI TTAA 3 cut(s) 648, 915, 1154
MslI CAYNNNNRTG 2 cut(s) 326, 446
MspA1I CMGCKG 2 cut(s) 769, 1232
MspI CCGG 5 cut(s) 29, 446, 459, 840, 1082
MspR9I CCNGG 3 cut(s) 122, 446, 532
Mva1269I GAATGC 2 cut(s) 508, 989
MvaI CCWGG 2 cut(s) 122, 532
MvnI CGCG 5 cut(s) 106, 129, 168, 291, 1232
MwoI GCNNNNNNNGC 2 cut(s) 698, 775
NaeI GCCGGC 2 cut(s) 30, 460
NarI GGCGCC 1 cut(s) 137
NciI CCSGG 1 cut(s) 446
NgoMIV GCCGGC 2 cut(s) 28, 458
NlaIII CATG 1 cut(s) 728
NlaIV GGNNCC 3 cut(s) 138, 297, 688
NmuCI GTSAC 2 cut(s) 319, 1028
NsbI TGCGCA 1 cut(s) 408
OliI CACNNNNGTG 2 cut(s) 326, 446
PceI AGGCCT 1 cut(s) 1195
PctI GAATGC 2 cut(s) 508, 989
PdiI GCCGGC 2 cut(s) 30, 460
PdmI GAANNNNTTC 1 cut(s) 635
PfeI GAWTC 2 cut(s) 772, 787
Pfl23II CGTACG 1 cut(s) 144
PflFI GACNNNGTC 1 cut(s) 1145
PfoI TCCNGGA 1 cut(s) 120
PkrI GCNGC 7 cut(s) 141, 160, 163, 182, 856, 1108, 1333
PleI GAGTC 2 cut(s) 179, 811
PluTI GGCGCC 1 cut(s) 140
PpsI GAGTC 2 cut(s) 179, 811
PshAI GACNNNNGTC 1 cut(s) 821
Psp6I CCWGG 2 cut(s) 120, 530
PspGI CCWGG 2 cut(s) 120, 530
PspLI CGTACG 1 cut(s) 144
PspN4I GGNNCC 3 cut(s) 138, 297, 688
PspPI GGNCC 6 cut(s) 124, 469, 574, 686, 737, 933
PstI CTGCAG 2 cut(s) 780, 856
PsuI RGATCY 1 cut(s) 295
PsyI GACNNNGTC 1 cut(s) 1145
PvuII CAGCTG 1 cut(s) 769
RsaI GTAC 6 cut(s) 146, 356, 1016, 1088, 1248, 1285
RsaNI GTAC 6 cut(s) 145, 355, 1015, 1087, 1247, 1284
RseI CAYNNNNRTG 2 cut(s) 326, 446
SacII CCGCGG 1 cut(s) 1233
SaqAI TTAA 3 cut(s) 648, 915, 1154
SatI GCNGC 7 cut(s) 140, 159, 162, 181, 855, 1107, 1332
Sau96I GGNCC 6 cut(s) 124, 469, 574, 686, 737, 933
ScaI AGTACT 2 cut(s) 1248, 1285
SchI GAGTC 2 cut(s) 180, 811
ScrFI CCNGG 3 cut(s) 122, 446, 532
SfaNI GCATC 4 cut(s) 257, 445, 943, 1066
SfcI CTRYAG 2 cut(s) 776, 852
SfoI GGCGCC 1 cut(s) 138
Sfr303I CCGCGG 1 cut(s) 1233
SgrBI CCGCGG 1 cut(s) 1233
SinI GGWCC 4 cut(s) 124, 469, 737, 933
SmiMI CAYNNNNRTG 2 cut(s) 326, 446
SpeI ACTAGT 1 cut(s) 1025
SseBI AGGCCT 1 cut(s) 1195
SspDI GGCGCC 1 cut(s) 136
SspMI CTAG 3 cut(s) 599, 927, 1026
StuI AGGCCT 1 cut(s) 1195
StyD4I CCNGG 3 cut(s) 120, 444, 530
StyI CCWWGG 2 cut(s) 228, 485
TaaI ACNGT 3 cut(s) 85, 1043, 1145
TaiI ACGT 1 cut(s) 150
TaqI TCGA 5 cut(s) 63, 114, 210, 264, 301
TatI WGTACW 3 cut(s) 1086, 1246, 1283
TauI GCSGC 3 cut(s) 142, 164, 183
TfiI GAWTC 2 cut(s) 772, 787
Tru1I TTAA 3 cut(s) 648, 915, 1154
Tru9I TTAA 3 cut(s) 648, 915, 1154
TscAI CASTG 2 cut(s) 328, 356
TseFI GTSAC 2 cut(s) 319, 1028
TseI GCWGC 4 cut(s) 158, 854, 1106, 1331
Tsp45I GTSAC 2 cut(s) 319, 1028
TspDTI ATGAA 5 cut(s) 687, 819, 982, 1164, 1220
TspGWI ACGGA 2 cut(s) 954, 1304
TspRI CASTG 2 cut(s) 328, 356
Tth111I GACNNNGTC 1 cut(s) 1145
VpaK11BI GGWCC 4 cut(s) 124, 469, 737, 933
XapI RAATTY 6 cut(s) 11, 303, 364, 644, 674, 718
XmnI GAANNNNTTC 1 cut(s) 635
XspI CTAG 3 cut(s) 599, 927, 1026
ZrmI AGTACT 2 cut(s) 1248, 1285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.