Rh1AG093700

alpha-N-acetylglucosaminidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000933
Physical Location & Seq
Reverse (-)
16318572 .. 16332936
14365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG093700.1

Sequence Viewer

Length: 528 bp
ATGAGAGCTGACCTCAAGATCTCTTTGATTTCGTCAGCAGCAAACACTTTCAAGTCATGGGAAAGGCTTGATGTAGGTGGCATTATATCCCACTCTTTTGAATTGGAGGCCAAGACGAAAGGACTGTTTAGTGAATGTGGAAGGACCAGTCACATATACAATTGTTATTCTTTCGATGAGAATACTCCACTTGTTGATGATCCAGAGTACATCTCTGCATTGGGTGAAACAATATTCAAGGGACTACAAAGCGGAGATAAGGATGGGATATGGCTAATGCAGGGTTGCTGTGAGGCCTATGCTAGAGCTATAGGCAGGTGTCAACAGCTAGAGAGGCTAGTTGTGGGGAGCTGTGCGCAGGACTCGACAGGAGCTGTCGGCAGGGGGCAGGCACAAGGCAGGGGCCGGTTCAGATTTTCCGATGGCCGGTTTAGGAGGATTTCGGCCGGTTCCGGTGGTTCCGCCACCAGTTCTGGGCTCCTTAGGACTAGGGCTTCCATATGTGGGGCGGTGGTTGCAGGATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
NAGLU NAGLU_C NAGLU_N

Protein Analysis

175

Amino Acids

18.78

Weight (kDa)

8.51

Isoelectric Point (pI)

24.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAGLU PF05089 47 - 104 1.9e-08 Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 306
Acc16I TGCGCA 1 cut(s) 357
Acc36I ACCTGC 1 cut(s) 306
AciI CCGC 3 cut(s) 252, 462, 509
AclWI GGATC 1 cut(s) 194
AcoI YGGCCR 2 cut(s) 424, 444
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 3 cut(s) 426, 474, 504
AgsI TTSAA 3 cut(s) 52, 101, 238
AluBI AGCT 5 cut(s) 8, 308, 328, 351, 374
AluI AGCT 5 cut(s) 8, 308, 328, 351, 374
AlwI GGATC 1 cut(s) 194
AlwNI CAGNNNCTG 1 cut(s) 374
AoxI GGCC 5 cut(s) 108, 294, 403, 424, 444
ApeKI GCWGC 1 cut(s) 38
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 2 cut(s) 144, 403
AsuHPI GGTGA 1 cut(s) 236
AvaII GGWCC 1 cut(s) 144
AxyI CCTNAGG 1 cut(s) 482
BanII GRGCYC 1 cut(s) 480
BbvI GCAGC 1 cut(s) 50
BccI CCATC 2 cut(s) 257, 416
BfaI CTAG 4 cut(s) 303, 329, 338, 489
BfmI CTRYAG 1 cut(s) 309
BfuAI ACCTGC 1 cut(s) 306
BglII AGATCT 1 cut(s) 18
BisI GCNGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 40
Bme18I GGWCC 1 cut(s) 144
BmgT120I GGNCC 2 cut(s) 144, 403
BmiI GGNNCC 4 cut(s) 404, 451, 460, 479
BplI GAGNNNNNCTC 3 cut(s) 29, 197, 229
BsaWI WCCGGW 1 cut(s) 452
Bsc4I CCNNNNNNNGG 3 cut(s) 426, 474, 504
Bse118I RCCGGY 3 cut(s) 405, 426, 446
Bse1I ACTGG 2 cut(s) 147, 468
Bse21I CCTNAGG 1 cut(s) 482
BseGI GGATG 1 cut(s) 268
BseLI CCNNNNNNNGG 3 cut(s) 426, 474, 504
BseNI ACTGG 2 cut(s) 147, 468
BseX3I CGGCCG 1 cut(s) 444
BseXI GCAGC 1 cut(s) 50
Bsh1285I CGRYCG 1 cut(s) 447
BshFI GGCC 5 cut(s) 110, 296, 405, 426, 446
BsiEI CGRYCG 1 cut(s) 447
BsiSI CCGG 4 cut(s) 406, 427, 447, 453
BslFI GGGAC 1 cut(s) 255
BslI CCNNNNNNNGG 3 cut(s) 426, 474, 504
BsmFI GGGAC 1 cut(s) 255
BsnI GGCC 5 cut(s) 110, 296, 405, 426, 446
Bsp1286I GDGCHC 1 cut(s) 480
Bsp143I GATC 2 cut(s) 18, 199
BspACI CCGC 3 cut(s) 252, 462, 509
BspANI GGCC 5 cut(s) 110, 296, 405, 426, 446
BspLI GGNNCC 4 cut(s) 404, 451, 460, 479
BspMI ACCTGC 1 cut(s) 306
BspPI GGATC 1 cut(s) 194
BsrFI RCCGGY 3 cut(s) 405, 426, 446
BsrI ACTGG 2 cut(s) 147, 468
BssAI RCCGGY 3 cut(s) 405, 426, 446
BssMI GATC 2 cut(s) 18, 199
Bst4CI ACNGT 1 cut(s) 126
BstC8I GCNNGC 1 cut(s) 390
BstDEI CTNAG 1 cut(s) 482
BstF5I GGATG 1 cut(s) 268
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 2 cut(s) 21, 202
BstMBI GATC 2 cut(s) 18, 199
BstMCI CGRYCG 1 cut(s) 447
BstMWI GCNNNNNNNGC 2 cut(s) 334, 515
BstSFI CTRYAG 1 cut(s) 309
BstV1I GCAGC 1 cut(s) 50
BstX2I RGATCY 1 cut(s) 18
BstYI RGATCY 1 cut(s) 18
BstZI CGGCCG 1 cut(s) 444
Bsu36I CCTNAGG 1 cut(s) 482
BsuRI GGCC 5 cut(s) 110, 296, 405, 426, 446
BtsCI GGATG 1 cut(s) 268
BveI ACCTGC 1 cut(s) 306
Cac8I GCNNGC 1 cut(s) 390
CaiI CAGNNNCTG 1 cut(s) 374
CfoI GCGC 1 cut(s) 358
Cfr10I RCCGGY 3 cut(s) 405, 426, 446
Cfr13I GGNCC 2 cut(s) 144, 403
Csp6I GTAC 1 cut(s) 208
CviAII CATG 1 cut(s) 57
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 1 cut(s) 482
DpnI GATC 2 cut(s) 20, 201
DpnII GATC 2 cut(s) 18, 199
EaeI YGGCCR 2 cut(s) 424, 444
EagI CGGCCG 1 cut(s) 444
EciI GGCGGA 1 cut(s) 451
EclXI CGGCCG 1 cut(s) 444
Eco147I AGGCCT 1 cut(s) 296
Eco24I GRGCYC 1 cut(s) 480
Eco47I GGWCC 1 cut(s) 144
Eco52I CGGCCG 1 cut(s) 444
Eco81I CCTNAGG 1 cut(s) 482
EcoT38I GRGCYC 1 cut(s) 480
FaeI CATG 1 cut(s) 60
FaiI YATR 9 cut(s) 58, 86, 155, 157, 271, 300, 311, 500, 502
FaqI GGGAC 1 cut(s) 255
FatI CATG 1 cut(s) 56
FauNDI CATATG 1 cut(s) 500
Fnu4HI GCNGC 1 cut(s) 39
FokI GGATG 1 cut(s) 275
FriOI GRGCYC 1 cut(s) 480
Fsp4HI GCNGC 1 cut(s) 39
FspBI CTAG 4 cut(s) 303, 329, 338, 489
FspI TGCGCA 1 cut(s) 357
GlaI GCGC 1 cut(s) 357
GluI GCNGC 1 cut(s) 39
HaeIII GGCC 5 cut(s) 110, 296, 405, 426, 446
HapII CCGG 4 cut(s) 406, 427, 447, 453
HhaI GCGC 1 cut(s) 358
Hin1II CATG 1 cut(s) 60
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HincII GTYRAC 1 cut(s) 323
HindII GTYRAC 1 cut(s) 323
HinfI GANTC 1 cut(s) 362
HpaII CCGG 4 cut(s) 406, 427, 447, 453
HphI GGTGA 1 cut(s) 236
Hpy166II GTNNAC 1 cut(s) 323
Hpy188I TCNGA 2 cut(s) 413, 421
Hpy188III TCNNGA 2 cut(s) 16, 203
Hpy8I GTNNAC 1 cut(s) 323
HpyAV CCTTC 1 cut(s) 135
HpyCH4III ACNGT 1 cut(s) 126
HpyCH4V TGCA 3 cut(s) 218, 280, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 334, 515
HpyF3I CTNAG 1 cut(s) 482
Hsp92II CATG 1 cut(s) 60
HspAI GCGC 1 cut(s) 356
Kzo9I GATC 2 cut(s) 18, 199
LmnI GCTCC 3 cut(s) 348, 371, 483
Lsp1109I GCAGC 1 cut(s) 50
MaeI CTAG 4 cut(s) 303, 329, 338, 489
MaeIII GTNAC 1 cut(s) 149
MalI GATC 2 cut(s) 20, 201
MboI GATC 2 cut(s) 18, 199
MfeI CAATTG 1 cut(s) 160
MflI RGATCY 1 cut(s) 18
MhlI GDGCHC 1 cut(s) 480
MluCI AATT 2 cut(s) 101, 160
MlyI GAGTC 1 cut(s) 356
MnlI CCTC 5 cut(s) 23, 100, 286, 327, 429
MspI CCGG 4 cut(s) 406, 427, 447, 453
MunI CAATTG 1 cut(s) 160
MwoI GCNNNNNNNGC 2 cut(s) 334, 515
NdeI CATATG 1 cut(s) 500
NdeII GATC 2 cut(s) 18, 199
NlaIII CATG 1 cut(s) 60
NlaIV GGNNCC 4 cut(s) 404, 451, 460, 479
NmuCI GTSAC 1 cut(s) 149
NsbI TGCGCA 1 cut(s) 357
PaqCI CACCTGC 1 cut(s) 306
PceI AGGCCT 1 cut(s) 296
PkrI GCNGC 1 cut(s) 40
PleI GAGTC 1 cut(s) 356
PpsI GAGTC 1 cut(s) 356
PspN4I GGNNCC 4 cut(s) 404, 451, 460, 479
PspPI GGNCC 2 cut(s) 144, 403
PstNI CAGNNNCTG 1 cut(s) 374
PsuI RGATCY 1 cut(s) 18
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
SatI GCNGC 1 cut(s) 39
Sau3AI GATC 2 cut(s) 18, 199
Sau96I GGNCC 2 cut(s) 144, 403
SchI GAGTC 1 cut(s) 356
SduI GDGCHC 1 cut(s) 480
SetI ASST 8 cut(s) 10, 15, 79, 310, 320, 330, 353, 376
SfcI CTRYAG 1 cut(s) 309
SinI GGWCC 1 cut(s) 144
SmlI CTYRAG 1 cut(s) 14
SmoI CTYRAG 1 cut(s) 14
Sse9I AATT 2 cut(s) 101, 160
SseBI AGGCCT 1 cut(s) 296
SsiI CCGC 3 cut(s) 252, 462, 509
SspI AATATT 1 cut(s) 234
SspMI CTAG 4 cut(s) 303, 329, 338, 489
StuI AGGCCT 1 cut(s) 296
TaaI ACNGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 174, 365
TasI AATT 2 cut(s) 101, 160
TatI WGTACW 1 cut(s) 207
TseFI GTSAC 1 cut(s) 149
TseI GCWGC 1 cut(s) 38
Tsp45I GTSAC 1 cut(s) 149
VpaK11BI GGWCC 1 cut(s) 144
XspI CTAG 4 cut(s) 303, 329, 338, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.