Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0000933

Physical Location & Seq

Genomic Coordinates Reverse (-)

3334274 .. 3343544

Gene Structure 9271 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G001880.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1956 bp

ATGTCGAATTCCATAGCCCAGTTGATGTTTCTGATTCTTGTCTTTGTACCAATAGCTTTATCATCAGAATCACAACAACCAGTCGAGGCTTTGCTGCGTCGTCTGGACTCCAAACGCTCCTCTGCTTCAGTTCAAGAAGCTGCAGCTAAAGCTGTTCTCAAACGATTGCTTCCTACCCATGTCGACAGTTTCGACTTCAAGATTGTTGACAAGGATGTTTGTGGTGGACATAGTTGCTTTGTGATAAACAATCATAACCTGTCAAGTCGAAATGGCCCCGAGATACAGATTAAAGGTACCACAGCAGTTGAAATTGCTTCTGGCCTCCATTGGTATTTGAAGTATTGGTGTGGTGCTCATGTTTCTTGGGACAAGACTGGTGGCGTGCAATTAGCTTCCATCCCCAATCCAGGATCACTACCTCGTGTAAAAGACAAGGGGTTGAAAATTCAACGGCCGGTCCCATGGAACTATTACCAAAATGTCGTTACATCCAGTTATTCATTTGTTTGGTGGGATTGGGAAAGATGGCAGAAAGAAATAGACTGGATGGCTCTTCAGGGGATCAACCTGCCTTTAGCATTCACAGGGCAAGAATCAATTTGGCAGAAAGTTTTCATGGATTTCAATATTAGTAAGGGAGACTTGAATGATTTCTTTGGTGGACCTGCCTTCCTTGCATGGGCTCGCATGGGGAATTTACATGCGTGGGGTGGACCATTATCACAGAATTGGTTGGATCAACAATTGGTTTTGCAGAAACAGATATTATCCCGGATGTTAGAGCTAGGCATGACTCCAGTGCTGCCATCATTCTCTGGAAATGTCCCAGCGACACTGAAGAAATTTTATCCTTCAGCTAATATAACCAGGCTTGGAGACTGGAACACTGTCAATGGTGATCGCCGCTGGTGCTGTACTTACCTTCTTGATCCTTCTGATCCTTTATTTGTTGAAATTGGGACTGCTTTTATTAGGCGACAAGTTGAAGAATACGGGGATGTGACGGACATCTATAACTGTGATACATTCAATGAAAATTCTCCACCTACAAATGATCCGGCTTATATTTCTTCACTTGGAGCTGCTGTCTACAAAGCAATGTCCAAAGGTGATACGGATGCAGTGTGGTTGATGCAAGGTTGGCTCTTCTACTCAGACTCTGCTTTTTGGAAACCACCTCAAATGAAGGCCCTTCTACATTCTGTTCCATTCGGGAAAATGATTGTACTTGATCTATTTGCTGATGTGAAACCAATATGGAACACGTCATCTCAATTTTACGACACTCCCTATATCTGGTGTCTCCTGCACAATTTTGGTGGGAACCTTGAAATGTATGGGATATTGGATGCAATTTCTTCAGGTCCAGTTGACGCCCGAACTAGTGAAAATTCTACTATGGTTGGGGTTGGCATGTGCATGGAAGGAATCGAGCACAATCCAGTTATCTATGAATTGACGTCTGAAATGGCATTTCGCAGTGAAAGGGTTCCAGTTCAGGATTGGCTGAGGACCTACTCCCGTAGACGTTACGGTAATGCAGTTCATCAAGTTGAGGAGGCTTGGGAAATTCTTCATCGCACAATTTATAACTGCACAGATGGAATTGCTGACCATAACACAGATTTCATAGTCAAATTACCTGACTGGGATCCATCACTAGAGAGTGTATCGAACACAACAGAACCTAAGAAGACGCAAATGCTTTTCACACTAGATATAAAGAGGAGATTTTTACTCCAAAAAAACAGCTCTCAATTTCCCAAGGCACATTTGTGGTATTCAACTCAGGATGTGATTAATGCTTTACGGTTATTTCTTGATGCTGGAAATGATCTTTCCGGAAGCCTTACATACAGATATGATTTGGTTGATTTAACACGCCAAGTGCTATCAAAGCTAGCAAACCAAGTATATGTGGACGCAGTGACTGCTTTTCAGAGGAAGATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051302 GO:0051781 GO:0061458 GO:0065007

KEGG Pathways

Metabolic & Signaling

ko00531 ko01100 ko04142 map00531 map01100 map04142

Pfam Domains

Protein Families

NAGLU NAGLU_C NAGLU_N

Protein Analysis

651

Amino Acids

73.96

Weight (kDa)

6.04

Isoelectric Point (pI)

47.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAGLU_N	PF12971	47 - 141	2.4e-24	Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain
NAGLU	PF05089	158 - 494	2.8e-146	Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
NAGLU_C	PF12972	503 - 650	3.8e-34	Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000933)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13690 AT5G13690
fragaria_vesca	FvH4_1g18460 FvH4_1g19570 FvH4_1g19580 FvH4_5g16380 FvH4_5g16380 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470
malus_domestica	MD07G1018500.v1.1 MD07G1018700.v1.1 MD14G1187100.v1.1
prunus_persica	Prupe.2G020300_v2.0.a1 Prupe.2G020300_v2.0.a1 Prupe.2G020300_v2.0.a1 Prupe.2G020300_v2.0.a1 Prupe.5G179300_v2.0.a1 Prupe.5G179300_v2.0.a1
pyrus_communis	pycom07g01480 pycom14g15500
rosa_chinensis	RchiOBHm_Chr1g0317311 RchiOBHm_Chr7g0179731 RchiOBHm_Chr7g0179741
rosa_laevigata	RLG00000005326 RLG00000017711 RLG00000029569 RLG00000030607
rosa_multiflora	Rmu_sc0000186.1_g000002 Rmu_sc0004156.1_g000003 Rmu_sc0005092.1_g000001 Rmu_sc0013869.1_g000005 Rmu_sc0026282.1_g000001
rosa_roxburghii	Rroxscaffold_3G00273510 Rroxscaffold_4G00330500
rosa_rugosa	Rorug01G0015800 Rorug02G0157200 Rorug06G0427700
rosa_samantha	Rh1AG027200 Rh1AG093700 Rh1BG023100 Rh1CG025200 Rh1DG046000 Rh2AG208700 Rh4CG293700 Rh7AG027200 Rh7AG027300 Rh7BG026300 Rh7BG026400 Rh7CG028400 Rh7CG170000 Rh7DG028000
rosa_wichuraiana	Rw1G001880 Rw7G002190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1593
AatII	GACGTC	1 cut(s)	1466
Acc36I	ACCTGC	2 cut(s)	579, 676
Acc65I	GGTACC	1 cut(s)	296
AccB1I	GGYRCC	1 cut(s)	296
AccI	GTMKAC	3 cut(s)	183, 1092, 1528
AccIII	TCCGGA	1 cut(s)	1844
AciI	CCGC	1 cut(s)	907
AclWI	GGATC	8 cut(s)	421, 572, 747, 926, 935, 1052, 1649, 1662
AcoI	YGGCCR	1 cut(s)	455
AcsI	RAATTY	7 cut(s)	7, 447, 697, 845, 1039, 1393, 1572
AcuI	CTGAAG	5 cut(s)	111, 542, 840, 860, 1347
AcyI	GRCGYC	2 cut(s)	1377, 1463
AfaI	GTAC	4 cut(s)	48, 298, 919, 1230
AfiI	CCNNNNNNNGG	3 cut(s)	410, 682, 1299
AflIII	ACRYGT	1 cut(s)	1266
AhlI	ACTAGT	1 cut(s)	1385
AjiI	CACGTC	1 cut(s)	1269
AjnI	CCWGG	2 cut(s)	409, 869
AleI	CACNNNNGTG	1 cut(s)	1777
Alw21I	GWGCWC	2 cut(s)	358, 1440
Alw26I	GTCTC	3 cut(s)	636, 873, 1310
AlwI	GGATC	8 cut(s)	421, 572, 747, 926, 935, 1052, 1649, 1662
AlwNI	CAGNNNCTG	2 cut(s)	1163, 1934
Ama87I	CYCGRG	1 cut(s)	278
Aor13HI	TCCGGA	1 cut(s)	1844
AoxI	GGCC	4 cut(s)	274, 322, 455, 1191
ApeKI	GCWGC	5 cut(s)	94, 140, 143, 805, 1085
ApoI	RAATTY	7 cut(s)	7, 447, 697, 845, 1039, 1393, 1572
AseI	ATTAAT	1 cut(s)	1803
Asp700I	GAANNNNTTC	4 cut(s)	614, 653, 1491, 1575
Asp718I	GGTACC	1 cut(s)	296
AspS9I	GGNCC	7 cut(s)	275, 460, 665, 716, 1192, 1367, 1515
AsuC2I	CCSGG	1 cut(s)	775
AsuHPI	GGTGA	2 cut(s)	911, 1124
AsuNHI	GCTAGC	1 cut(s)	1903
AvaI	CYCGRG	1 cut(s)	278
AvaII	GGWCC	5 cut(s)	460, 665, 716, 1367, 1515
BamHI	GGATCC	1 cut(s)	1654
BanI	GGYRCC	1 cut(s)	296
BanII	GRGCYC	1 cut(s)	688
BauI	CACGAG	1 cut(s)	423
BbsI	GAAGAC	1 cut(s)	1703
Bbv12I	GWGCWC	2 cut(s)	358, 1440
BbvCI	CCTCAGC	1 cut(s)	1511
BbvI	GCAGC	5 cut(s)	81, 127, 155, 792, 1072
BccI	CCATC	6 cut(s)	407, 522, 544, 817, 1598, 1666
BceAI	ACGGC	1 cut(s)	470
BciT130I	CCWGG	2 cut(s)	411, 871
BcnI	CCSGG	1 cut(s)	775
BcoDI	GTCTC	3 cut(s)	636, 873, 1310
BcuI	ACTAGT	1 cut(s)	1385
BfaI	CTAG	5 cut(s)	788, 1386, 1664, 1718, 1904
BfmI	CTRYAG	1 cut(s)	141
BfuAI	ACCTGC	2 cut(s)	579, 676
BisI	GCNGC	6 cut(s)	95, 141, 144, 806, 907, 1086
BlsI	GCNGC	6 cut(s)	96, 142, 145, 807, 908, 1087
Bme1390I	CCNGG	3 cut(s)	411, 775, 871
Bme18I	GGWCC	5 cut(s)	460, 665, 716, 1367, 1515
BmeT110I	CYCGRG	1 cut(s)	278
BmgBI	CACGTC	1 cut(s)	1269
BmgT120I	GGNCC	7 cut(s)	275, 460, 665, 716, 1192, 1367, 1515
BmiI	GGNNCC	6 cut(s)	277, 298, 462, 1328, 1494, 1656
BmrFI	CCNGG	3 cut(s)	411, 775, 871
BmrI	ACTGGG	2 cut(s)	13, 1660
BmsI	GCATC	4 cut(s)	1111, 1125, 1342, 1816
BmtI	GCTAGC	1 cut(s)	1907
BmuI	ACTGGG	2 cut(s)	13, 1660
BpiI	GAAGAC	1 cut(s)	1703
BpmI	CTGGAG	1 cut(s)	783
Bpu10I	CCTNAGC	1 cut(s)	1511
BpuMI	CCSGG	1 cut(s)	775
BsaHI	GRCGYC	2 cut(s)	1377, 1463
BsaJI	CCNNGG	2 cut(s)	464, 1767
BsaWI	WCCGGW	1 cut(s)	1844
BsaXI	ACNNNNNCTCC	2 cut(s)	1074, 1104
Bsc4I	CCNNNNNNNGG	3 cut(s)	410, 682, 1299
Bse118I	RCCGGY	1 cut(s)	457
Bse3DI	GCAATG	1 cut(s)	1107
BseAI	TCCGGA	1 cut(s)	1844
BseBI	CCWGG	2 cut(s)	411, 871
BseDI	CCNNGG	2 cut(s)	464, 1767
BseGI	GGATG	9 cut(s)	220, 399, 491, 555, 783, 1006, 1126, 1357, 1801
BseLI	CCNNNNNNNGG	3 cut(s)	410, 682, 1299
BseMI	GCAATG	1 cut(s)	1107
BseMII	CTCAG	3 cut(s)	1170, 1502, 1805
BseRI	GAGGAG	3 cut(s)	109, 1574, 1744
BseX3I	CGGCCG	1 cut(s)	455
BseXI	GCAGC	5 cut(s)	81, 127, 155, 792, 1072
BseYI	CCCAGC	1 cut(s)	829
BsgI	GTGCAG	2 cut(s)	1295, 1582
Bsh1285I	CGRYCG	1 cut(s)	458
BshFI	GGCC	4 cut(s)	276, 324, 457, 1193
BshNI	GGYRCC	1 cut(s)	296
BsiEI	CGRYCG	1 cut(s)	458
BsiHKAI	GWGCWC	2 cut(s)	358, 1440
BsiHKCI	CYCGRG	1 cut(s)	278
BsiSI	CCGG	4 cut(s)	458, 775, 1061, 1845
BslFI	GGGAC	4 cut(s)	383, 446, 812, 976
BslI	CCNNNNNNNGG	3 cut(s)	410, 682, 1299
BsmAI	GTCTC	3 cut(s)	636, 873, 1310
BsmFI	GGGAC	4 cut(s)	383, 446, 812, 976
BsmI	GAATGC	1 cut(s)	581
BsnI	GGCC	4 cut(s)	276, 324, 457, 1193
BsoBI	CYCGRG	1 cut(s)	278
Bsp1286I	GDGCHC	3 cut(s)	358, 688, 1440
Bsp13I	TCCGGA	1 cut(s)	1844
Bsp19I	CCATGG	1 cut(s)	464
BspACI	CCGC	1 cut(s)	907
BspANI	GGCC	4 cut(s)	276, 324, 457, 1193
BspCNI	CTCAG	3 cut(s)	1169, 1503, 1804
BspEI	TCCGGA	1 cut(s)	1844
BspLI	GGNNCC	6 cut(s)	277, 298, 462, 1328, 1494, 1656
BspMAI	CTGCAG	1 cut(s)	145
BspMI	ACCTGC	2 cut(s)	579, 676
BspOI	GCTAGC	1 cut(s)	1907
BspPI	GGATC	8 cut(s)	421, 572, 747, 926, 935, 1052, 1649, 1662
BspQI	GCTCTTC	2 cut(s)	561, 1154
BspT107I	GGYRCC	1 cut(s)	296
BsrDI	GCAATG	1 cut(s)	1107
BsrFI	RCCGGY	1 cut(s)	457
BssAI	RCCGGY	1 cut(s)	457
BssECI	CCNNGG	2 cut(s)	464, 1767
BssNI	GRCGYC	2 cut(s)	1377, 1463
BssSI	CACGAG	1 cut(s)	423
BssT1I	CCWWGG	2 cut(s)	464, 1767
Bst2BI	CACGAG	1 cut(s)	423
Bst2UI	CCWGG	2 cut(s)	411, 871
Bst4CI	ACNGT	5 cut(s)	188, 892, 1022, 1538, 1815
Bst6I	CTCTTC	2 cut(s)	561, 1154
BstACI	GRCGYC	2 cut(s)	1377, 1463
BstAPI	GCANNNNNTGC	1 cut(s)	1934
BstC8I	GCNNGC	3 cut(s)	386, 688, 1905
BstDEI	CTNAG	4 cut(s)	1156, 1511, 1692, 1791
BstDSI	CCRYGG	1 cut(s)	464
BstF5I	GGATG	9 cut(s)	220, 399, 491, 555, 783, 1006, 1126, 1357, 1801
BstMAI	GTCTC	3 cut(s)	636, 873, 1310
BstMCI	CGRYCG	1 cut(s)	458
BstMWI	GCNNNNNNNGC	6 cut(s)	149, 677, 912, 1144, 1900, 1934
BstNI	CCWGG	2 cut(s)	411, 871
BstNSI	RCATGY	2 cut(s)	707, 1420
BstSCI	CCNGG	3 cut(s)	409, 773, 869
BstSFI	CTRYAG	1 cut(s)	141
BstV1I	GCAGC	5 cut(s)	81, 127, 155, 792, 1072
BstV2I	GAAGAC	1 cut(s)	1703
BstX2I	RGATCY	1 cut(s)	1654
BstYI	RGATCY	1 cut(s)	1654
BstZI	CGGCCG	1 cut(s)	455
BsuRI	GGCC	4 cut(s)	276, 324, 457, 1193
BtgI	CCRYGG	1 cut(s)	464
BtgZI	GCGATG	1 cut(s)	1565
BtrI	CACGTC	1 cut(s)	1269
BtsCI	GGATG	9 cut(s)	220, 399, 491, 555, 783, 1006, 1126, 1357, 1801
BtsI	GCAGTG	3 cut(s)	1131, 1489, 1935
BtsIMutI	CAGTG	6 cut(s)	807, 836, 888, 1131, 1489, 1935
BveI	ACCTGC	2 cut(s)	579, 676
Cac8I	GCNNGC	3 cut(s)	386, 688, 1905
CaiI	CAGNNNCTG	2 cut(s)	1163, 1934
Cfr10I	RCCGGY	1 cut(s)	457
Cfr13I	GGNCC	7 cut(s)	275, 460, 665, 716, 1192, 1367, 1515
CseI	GACGC	4 cut(s)	86, 1385, 1708, 1934
Csp6I	GTAC	4 cut(s)	47, 297, 918, 1229
CspCI	CAANNNNNGTGG	6 cut(s)	289, 324, 361, 396, 1303, 1338
CviQI	GTAC	4 cut(s)	47, 297, 918, 1229
DdeI	CTNAG	4 cut(s)	1156, 1511, 1692, 1791
EaeI	YGGCCR	1 cut(s)	455
EagI	CGGCCG	1 cut(s)	455
Eam1104I	CTCTTC	2 cut(s)	561, 1154
EarI	CTCTTC	2 cut(s)	561, 1154
EclXI	CGGCCG	1 cut(s)	455
Eco130I	CCWWGG	2 cut(s)	464, 1767
Eco24I	GRGCYC	1 cut(s)	688
Eco47I	GGWCC	5 cut(s)	460, 665, 716, 1367, 1515
Eco52I	CGGCCG	1 cut(s)	455
Eco57I	CTGAAG	5 cut(s)	111, 542, 840, 860, 1347
Eco88I	CYCGRG	1 cut(s)	278
EcoO109I	RGGNCCY	2 cut(s)	1192, 1515
EcoRI	GAATTC	1 cut(s)	7
EcoRII	CCWGG	2 cut(s)	409, 869
EcoT14I	CCWWGG	2 cut(s)	464, 1767
EcoT38I	GRGCYC	1 cut(s)	688
ErhI	CCWWGG	2 cut(s)	464, 1767
FalI	AAGNNNNNCTT	2 cut(s)	629, 661
FaqI	GGGAC	4 cut(s)	383, 446, 812, 976
FblI	GTMKAC	3 cut(s)	183, 1092, 1528
Fnu4HI	GCNGC	6 cut(s)	95, 141, 144, 806, 907, 1086
FokI	GGATG	9 cut(s)	227, 386, 478, 562, 790, 1013, 1133, 1364, 1808
FriOI	GRGCYC	1 cut(s)	688
Fsp4HI	GCNGC	6 cut(s)	95, 141, 144, 806, 907, 1086
FspBI	CTAG	5 cut(s)	788, 1386, 1664, 1718, 1904
GluI	GCNGC	6 cut(s)	95, 141, 144, 806, 907, 1086
GsaI	CCCAGC	1 cut(s)	833
GsuI	CTGGAG	1 cut(s)	783
HaeIII	GGCC	4 cut(s)	276, 324, 457, 1193
HapII	CCGG	4 cut(s)	458, 775, 1061, 1845
HgaI	GACGC	4 cut(s)	86, 1385, 1708, 1934
Hin1I	GRCGYC	2 cut(s)	1377, 1463
HincII	GTYRAC	3 cut(s)	184, 208, 1375
HindII	GTYRAC	3 cut(s)	184, 208, 1375
HinfI	GANTC	7 cut(s)	34, 68, 107, 596, 796, 1160, 1431
HpaII	CCGG	4 cut(s)	458, 775, 1061, 1845
HphI	GGTGA	2 cut(s)	911, 1124
Hpy166II	GTNNAC	9 cut(s)	184, 208, 227, 665, 716, 1093, 1375, 1529, 1924
Hpy188I	TCNGA	6 cut(s)	33, 67, 940, 1159, 1468, 1944
Hpy8I	GTNNAC	9 cut(s)	184, 208, 227, 665, 716, 1093, 1375, 1529, 1924
Hpy99I	CGWCG	1 cut(s)	102
HpyAV	CCTTC	7 cut(s)	682, 864, 935, 945, 1183, 1205, 1421
HpyCH4III	ACNGT	5 cut(s)	188, 892, 1022, 1538, 1815
HpyCH4IV	ACGT	3 cut(s)	1268, 1463, 1531
HpyF10VI	GCNNNNNNNGC	6 cut(s)	149, 677, 912, 1144, 1900, 1934
HpyF3I	CTNAG	4 cut(s)	1156, 1511, 1692, 1791
HpySE526I	ACGT	3 cut(s)	1268, 1463, 1531
Hsp92I	GRCGYC	2 cut(s)	1377, 1463
Kpn2I	TCCGGA	1 cut(s)	1844
KpnI	GGTACC	1 cut(s)	300
LguI	GCTCTTC	2 cut(s)	561, 1154
LmnI	GCTCC	2 cut(s)	122, 1082
Lsp1109I	GCAGC	5 cut(s)	81, 127, 155, 792, 1072
LweI	GCATC	4 cut(s)	1111, 1125, 1342, 1816
MaeI	CTAG	5 cut(s)	788, 1386, 1664, 1718, 1904
MaeII	ACGT	3 cut(s)	1268, 1463, 1531
MaeIII	GTNAC	4 cut(s)	487, 1003, 1532, 1930
MboII	GAAGA	8 cut(s)	548, 853, 1001, 1065, 1141, 1353, 1568, 1708
MfeI	CAATTG	1 cut(s)	746
MflI	RGATCY	1 cut(s)	1654
MhlI	GDGCHC	3 cut(s)	358, 688, 1440
MlyI	GAGTC	3 cut(s)	101, 790, 1154
MmeI	TCCRAC	1 cut(s)	717
MroI	TCCGGA	1 cut(s)	1844
MroXI	GAANNNNTTC	4 cut(s)	614, 653, 1491, 1575
MseI	TTAA	3 cut(s)	291, 1803, 1880
MslI	CAYNNNNRTG	2 cut(s)	1421, 1777
MspA1I	CMGCKG	1 cut(s)	909
MspI	CCGG	4 cut(s)	458, 775, 1061, 1845
MspR9I	CCNGG	3 cut(s)	411, 775, 871
MunI	CAATTG	1 cut(s)	746
Mva1269I	GAATGC	1 cut(s)	581
MvaI	CCWGG	2 cut(s)	411, 871
MwoI	GCNNNNNNNGC	6 cut(s)	149, 677, 912, 1144, 1900, 1934
NciI	CCSGG	1 cut(s)	775
NcoI	CCATGG	1 cut(s)	464
NheI	GCTAGC	1 cut(s)	1903
NlaIV	GGNNCC	6 cut(s)	277, 298, 462, 1328, 1494, 1656
NmuCI	GTSAC	2 cut(s)	1003, 1930
NspI	RCATGY	2 cut(s)	707, 1420
OliI	CACNNNNGTG	1 cut(s)	1777
PciSI	GCTCTTC	2 cut(s)	561, 1154
PcsI	WCGNNNNNNNCGW	1 cut(s)	189
PctI	GAATGC	1 cut(s)	581
PdmI	GAANNNNTTC	4 cut(s)	614, 653, 1491, 1575
PfeI	GAWTC	4 cut(s)	34, 68, 596, 1431
PfoI	TCCNGGA	2 cut(s)	409, 773
PkrI	GCNGC	6 cut(s)	96, 142, 145, 807, 908, 1087
PleI	GAGTC	3 cut(s)	101, 790, 1154
PpsI	GAGTC	3 cut(s)	101, 790, 1154
PpuMI	RGGWCCY	1 cut(s)	1515
PshBI	ATTAAT	1 cut(s)	1803
PsiI	TTATAA	1 cut(s)	1593
Psp5II	RGGWCCY	1 cut(s)	1515
Psp6I	CCWGG	2 cut(s)	409, 869
PspFI	CCCAGC	1 cut(s)	829
PspGI	CCWGG	2 cut(s)	409, 869
PspN4I	GGNNCC	6 cut(s)	277, 298, 462, 1328, 1494, 1656
PspPI	GGNCC	7 cut(s)	275, 460, 665, 716, 1192, 1367, 1515
PspPPI	RGGWCCY	1 cut(s)	1515
PstI	CTGCAG	1 cut(s)	145
PstNI	CAGNNNCTG	2 cut(s)	1163, 1934
PsuI	RGATCY	1 cut(s)	1654
RsaI	GTAC	4 cut(s)	48, 298, 919, 1230
RsaNI	GTAC	4 cut(s)	47, 297, 918, 1229
RseI	CAYNNNNRTG	2 cut(s)	1421, 1777
SalI	GTCGAC	1 cut(s)	182
SapI	GCTCTTC	2 cut(s)	561, 1154
SaqAI	TTAA	3 cut(s)	291, 1803, 1880
SatI	GCNGC	6 cut(s)	95, 141, 144, 806, 907, 1086
Sau96I	GGNCC	7 cut(s)	275, 460, 665, 716, 1192, 1367, 1515
SchI	GAGTC	3 cut(s)	101, 790, 1154
ScrFI	CCNGG	3 cut(s)	411, 775, 871
SduI	GDGCHC	3 cut(s)	358, 688, 1440
SfaNI	GCATC	4 cut(s)	1111, 1125, 1342, 1816
SfcI	CTRYAG	1 cut(s)	141
SinI	GGWCC	5 cut(s)	460, 665, 716, 1367, 1515
SmiMI	CAYNNNNRTG	2 cut(s)	1421, 1777
SpeI	ACTAGT	1 cut(s)	1385
SsiI	CCGC	1 cut(s)	907
SspI	AATATT	1 cut(s)	631
SspMI	CTAG	5 cut(s)	788, 1386, 1664, 1718, 1904
StyD4I	CCNGG	3 cut(s)	409, 773, 869
StyI	CCWWGG	2 cut(s)	464, 1767
TaaI	ACNGT	5 cut(s)	188, 892, 1022, 1538, 1815
TaiI	ACGT	3 cut(s)	1271, 1466, 1534
TaqI	TCGA	7 cut(s)	5, 84, 183, 192, 268, 1434, 1676
TatI	WGTACW	2 cut(s)	917, 1228
TauI	GCSGC	1 cut(s)	909
TfiI	GAWTC	4 cut(s)	34, 68, 596, 1431
Tru1I	TTAA	3 cut(s)	291, 1803, 1880
Tru9I	TTAA	3 cut(s)	291, 1803, 1880
TscAI	CASTG	6 cut(s)	807, 843, 895, 1131, 1489, 1935
TseFI	GTSAC	2 cut(s)	1003, 1930
TseI	GCWGC	5 cut(s)	94, 140, 143, 805, 1085
Tsp45I	GTSAC	2 cut(s)	1003, 1930
TspDTI	ATGAA	8 cut(s)	492, 607, 1050, 1202, 1470, 1538, 1568, 1621
TspGWI	ACGGA	2 cut(s)	1022, 1133
TspRI	CASTG	6 cut(s)	807, 843, 895, 1131, 1489, 1935
VpaK11BI	GGWCC	5 cut(s)	460, 665, 716, 1367, 1515
VspI	ATTAAT	1 cut(s)	1803
XapI	RAATTY	7 cut(s)	7, 447, 697, 845, 1039, 1393, 1572
XceI	RCATGY	2 cut(s)	707, 1420
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1503
XmiI	GTMKAC	3 cut(s)	183, 1092, 1528
XmnI	GAANNNNTTC	4 cut(s)	614, 653, 1491, 1575
XspI	CTAG	5 cut(s)	788, 1386, 1664, 1718, 1904
ZraI	GACGTC	1 cut(s)	1464

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.