MD14G1187100.v1.1

alpha-N-acetylglucosaminidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000933
Physical Location & Seq
Forward (+)
27941769 .. 27947032
5264 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1187100.v1.1.491

Sequence Viewer

Length: 2445 bp
ATGGCCTCCAAATTTCCCACCTTTTCTCCGGCCTTCACAATACTCCTTACTATCCTCTTCCTCGACTTGGCTTACTCGTCCACCGTCGGAGTCCGACACATTTCGCGCCTTCTCGAAATCCAGGACCGCGAGAAGGCGCCGCCGCACGTTCAAGTCGCTGCCGCTCGTGGAGTCCTCCGCCGCCTCCTCCCCTCTCACTCTTCCAGCTTCGACTTCCAAATCGTCTCCAAGGAGCAATGTGGGGGTGCATCTTGCTTTATGATCAAGAACAATCCTTCTTTTCGCAGACGCGGGGATCCTCAAATTCTAATTTCTGGGGTCACTGGAGTGGAGATTTTGGCTGGTCTGCACTGGTACTTGAAGAATTTGTGTAATTCACATATATCTTGGGATAAAACCGGCGGTGCGCAACTGTTTTCAGTGCCGAAATCAGGGTTGCTCACCCGGGTACAAGATGCCGGCATTTTGGTCCAGAGACCTGTTCCTTGGAACTACTACCAGAATGCTGTTACTTCCAGCTATACATTCGCTTGGTGGGATTGGGAAAGATGGGAGAAGGAGATTGACTGGATGGCCCTTCAAGGGATCAATTTGCCACTAGCATTTACAGGGCATGAGGCTATTTGGCAGAAAGTTTTCCAGAAATTTAATATGAGCAAATCAGACCTGGATGATTTTTTTGGGGGCCCCGCTTTCCTTGCTTGGTCTCGTATGGGAAATTTGCATGGATGGGGCGGACCTCTACCTCAAAGTTGGTTGGATCAACAGCTGATTCTGCAGAAAAAGATTCTGGTCAGAATGTATGAACTTGGAATGACTCCAGTCCTTCCAGCCTTTTCCGGAAATGTTCCTGCAGCGCTCAAAACTACATATCCATCAGCGAAGATAACACACTTAGGAAATTGGTTTTCTGTTAAGAGTGATCCTAGATGGACCTGCACTTACCTTCTTGATGCAACTGATCCGTTGTTTGTTGAAATTGGGAGAGCATTCATTGAGCAACAAGTAAAAGAGTACGGCAGGACTAGTCACATATACAACTGTGATACTTTTGATGAGAATACTCCACCTGACGATGCTCCGGAGTACATATCTTCATTAGGTGCAGCAATCTTTAGGGGTATGCAAAGTGGAGACAAGGAAGGTGTCTGGTTAATGCAGGGGTGGTTATTTTCATACGATCCATTCTGGAGGCCTCCACAAATGAAGGCACTTTTACACTCCGTTCCCACGGGGAAGCTGGTAGTACTTGATCTGTTTGCTGAAGTGAAACCTATATGGAGTACTACGGAGCAGTGTATGCTGCACAATTTTGCAGGAAATGTTGAGATGTACGGAGTTTTGGATGCAATTGCATCTGGACCAATCAATGCTCGTATAAGTGAAAACTCAACAATGGTTGGTGTCGGAATGTCAATGGAAGGTATAGAACAGAATCCTGTTGTTTATGATCTCATGTCTGAGATGGCTTTTCAACACAACAAAGTTGATGCTAAGGCCTGGATTGATCAATATTCTGCCAGACGTTATGGCCGTTCAACTCCTTCAATACAAGATGCCTGGAATATACTATATCATACCATCTATAACTGCACTGATGGTGCCTATGACAAAAACAGGGATGTGATTGTGGCATTTCCCGACGTTGATCCATCCTTAATATCAACACTGTCAGAAGGTCGTCATCAAAATGAGAAACCAGTAGCAGGAAGTGCAGTCCTCAAGGAAGTAACTGATTCGTTCGATCGACCTCATTTATGGTATTCAACTTCTGAAGTAATACATGCATTAGAACTCTTTATTGCAAGTGGAGATGAGCTCTCAGAAAGTAGTGCTTACAGGTATGATCTTGTTGATCTGACTAGACAAGCCTTATCTAAATATGCAAACCAGCTCTTTCTAAAGGTCATAGAGGCCTATCACTCAAATGATGTCCTCGGAGTGGCACGCGACAGCAAGAAGTTTTTAGACCTAGTTGAAGACATGGACGTACTCTTAGCCTGCCACGATGGATTTCTCCTAGGACCCTGGTTGGAAAGTGCGAAGAAACTCGCACAGGACGAAGAACAGGAAAAACAGTTTGAGTGGAATGCTAGAACACAGATAACAATGTGGTTCGACAACACGGAGGAGGAAGCAAGTCTGCTTCGCGACTATGGAAACAAGTACTGGAGCGGGCTTCTACGCGATTATTATGGCCCTCGAGCAGCTATATATTTCAAGTATTTAACGAAAAGCTTGGAGGAGGGCGGCGAGTTTGGATTGAAGGATTGGAGGAGGGAGTGGATAAAGCTGACGAATGATTGGCAAAACAGCAGGAAGCCGTTTCCTGTGAAAAGTACTGGAAACGCTCTCAACACATCCCGGTGGCTTTTTGATAAGTACTTATTAGGAAGTGCTTCTGACATACAACCACTTAATGAAGCTGCTTATCCGAGATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
NAGLU NAGLU_C NAGLU_N

Protein Analysis

815

Amino Acids

92.52

Weight (kDa)

5.65

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAGLU_N PF12971 53 - 141 1.8e-21 Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain
NAGLU PF05089 165 - 492 4.2e-134 Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
NAGLU_C PF12972 501 - 794 8e-90 Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 408
Acc36I ACCTGC 1 cut(s) 944
AccB1I GGYRCC 2 cut(s) 136, 1601
AccBSI CCGCTC 2 cut(s) 164, 2175
AccII CGCG 6 cut(s) 106, 129, 291, 1950, 2151, 2187
AccIII TCCGGA 2 cut(s) 839, 1081
AclWI GGATC 8 cut(s) 290, 303, 593, 768, 917, 956, 1175, 1643
AcoI YGGCCR 1 cut(s) 1531
AcsI RAATTY 5 cut(s) 11, 303, 364, 644, 718
AcuI CTGAAG 2 cut(s) 1284, 1794
AcyI GRCGYC 1 cut(s) 137
AfeI AGCGCT 1 cut(s) 858
AfiI CCNNNNNNNGG 6 cut(s) 67, 133, 431, 582, 1706, 1942
AhlI ACTAGT 1 cut(s) 1025
AjnI CCWGG 5 cut(s) 120, 666, 1499, 1559, 2027
AleI CACNNNNGTG 1 cut(s) 326
Alw21I GWGCWC 1 cut(s) 1821
Alw26I GTCTC 4 cut(s) 229, 469, 711, 1128
AlwI GGATC 8 cut(s) 290, 303, 593, 768, 917, 956, 1175, 1643
Ama87I CYCGRG 2 cut(s) 444, 2202
Aor13HI TCCGGA 2 cut(s) 839, 1081
Aor51HI AGCGCT 1 cut(s) 858
AoxI GGCC 9 cut(s) 3, 30, 573, 685, 1193, 1497, 1531, 1914, 2197
ApaI GGGCCC 1 cut(s) 689
ApeKI GCWGC 6 cut(s) 158, 854, 1106, 1303, 2207, 2426
ApoI RAATTY 5 cut(s) 11, 303, 364, 644, 718
Asp700I GAANNNNTTC 2 cut(s) 635, 2398
AspA2I CCTAGG 1 cut(s) 2020
AspLEI GCGC 4 cut(s) 108, 139, 409, 859
AsuC2I CCSGG 3 cut(s) 445, 446, 2365
AsuHPI GGTGA 1 cut(s) 433
AvaI CYCGRG 2 cut(s) 444, 2202
AvaII GGWCC 6 cut(s) 124, 469, 737, 933, 1361, 2024
AvrII CCTAGG 1 cut(s) 2020
BaeGI GKGCMC 1 cut(s) 689
BamHI GGATCC 1 cut(s) 295
BanI GGYRCC 2 cut(s) 136, 1601
BanII GRGCYC 2 cut(s) 689, 1821
BauI CACGAG 1 cut(s) 165
BbsI GAAGAC 1 cut(s) 1986
Bbv12I GWGCWC 1 cut(s) 1821
BbvI GCAGC 6 cut(s) 145, 866, 1118, 1290, 2219, 2413
BceAI ACGGC 3 cut(s) 1033, 1518, 2308
BciT130I CCWGG 5 cut(s) 122, 668, 1501, 1561, 2029
BclI TGATCA 2 cut(s) 261, 1507
BcnI CCSGG 3 cut(s) 445, 446, 2365
BcoDI GTCTC 4 cut(s) 229, 469, 711, 1128
BcuI ACTAGT 1 cut(s) 1025
BfaI CTAG 7 cut(s) 599, 927, 1026, 1863, 1973, 2021, 2094
BfmI CTRYAG 2 cut(s) 776, 852
BfoI RGCGCY 2 cut(s) 140, 860
BfuAI ACCTGC 1 cut(s) 944
BlnI CCTAGG 1 cut(s) 2020
BmcAI AGTACT 5 cut(s) 1248, 1285, 2168, 2341, 2384
Bme1390I CCNGG 8 cut(s) 122, 445, 446, 668, 1501, 1561, 2029, 2365
Bme18I GGWCC 6 cut(s) 124, 469, 737, 933, 1361, 2024
BmeT110I CYCGRG 2 cut(s) 444, 2202
BmiI GGNNCC 7 cut(s) 138, 297, 686, 687, 688, 1603, 2026
BmrFI CCNGG 8 cut(s) 122, 445, 446, 668, 1501, 1561, 2029, 2365
BmsI GCATC 8 cut(s) 257, 445, 943, 1066, 1336, 1364, 1480, 1546
BoxI GACNNNNGTC 1 cut(s) 821
BpiI GAAGAC 1 cut(s) 1986
BplI GAGNNNNNCTC 2 cut(s) 1803, 1835
BpmI CTGGAG 4 cut(s) 345, 804, 1210, 2191
Bpu10I CCTNAGC 1 cut(s) 1494
BpuEI CTTGAG 1 cut(s) 1706
BpuMI CCSGG 3 cut(s) 445, 446, 2365
BsaHI GRCGYC 1 cut(s) 137
BsaI GGTCTC 2 cut(s) 469, 711
BsaJI CCNNGG 7 cut(s) 228, 444, 485, 1230, 1936, 2020, 2027
BsaWI WCCGGW 2 cut(s) 839, 1081
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 6 cut(s) 67, 133, 431, 582, 1706, 1942
Bse118I RCCGGY 2 cut(s) 398, 458
Bse1I ACTGG 7 cut(s) 328, 356, 572, 821, 1700, 2174, 2347
Bse3DI GCAATG 1 cut(s) 242
BseAI TCCGGA 2 cut(s) 839, 1081
BseBI CCWGG 5 cut(s) 122, 668, 1501, 1561, 2029
BseDI CCNNGG 7 cut(s) 228, 444, 485, 1230, 1936, 2020, 2027
BseGI GGATG 7 cut(s) 576, 676, 734, 1351, 1627, 1652, 2360
BseLI CCNNNNNNNGG 6 cut(s) 67, 133, 431, 582, 1706, 1942
BseMI GCAATG 1 cut(s) 242
BseMII CTCAG 2 cut(s) 1452, 1836
BseNI ACTGG 7 cut(s) 328, 356, 572, 821, 1700, 2174, 2347
BseRI GAGGAG 4 cut(s) 176, 2144, 2258, 2290
BseSI GKGCMC 1 cut(s) 689
BseXI GCAGC 6 cut(s) 145, 866, 1118, 1290, 2219, 2413
BsgI GTGCAG 6 cut(s) 332, 922, 1125, 1289, 1576, 1734
Bsh1236I CGCG 6 cut(s) 106, 129, 291, 1950, 2151, 2187
Bsh1285I CGRYCG 1 cut(s) 1747
BshFI GGCC 9 cut(s) 5, 32, 575, 687, 1195, 1499, 1533, 1916, 2199
BshNI GGYRCC 2 cut(s) 136, 1601
BsiEI CGRYCG 1 cut(s) 1747
BsiHKAI GWGCWC 1 cut(s) 1821
BsiHKCI CYCGRG 2 cut(s) 444, 2202
BsiSI CCGG 7 cut(s) 29, 399, 445, 459, 840, 1082, 2365
BslI CCNNNNNNNGG 6 cut(s) 67, 133, 431, 582, 1706, 1942
BsmAI GTCTC 4 cut(s) 229, 469, 711, 1128
BsmBI CGTCTC 1 cut(s) 229
BsmI GAATGC 3 cut(s) 508, 989, 2095
BsnI GGCC 9 cut(s) 5, 32, 575, 687, 1195, 1499, 1533, 1916, 2199
Bso31I GGTCTC 2 cut(s) 469, 711
BsoBI CYCGRG 2 cut(s) 444, 2202
Bsp120I GGGCCC 1 cut(s) 685
Bsp1286I GDGCHC 2 cut(s) 689, 1821
Bsp13I TCCGGA 2 cut(s) 839, 1081
Bsp68I TCGCGA 1 cut(s) 2151
BspANI GGCC 9 cut(s) 5, 32, 575, 687, 1195, 1499, 1533, 1916, 2199
BspCNI CTCAG 2 cut(s) 1453, 1835
BspEI TCCGGA 2 cut(s) 839, 1081
BspFNI CGCG 6 cut(s) 106, 129, 291, 1950, 2151, 2187
BspLI GGNNCC 7 cut(s) 138, 297, 686, 687, 688, 1603, 2026
BspMAI CTGCAG 2 cut(s) 780, 856
BspMI ACCTGC 1 cut(s) 944
BspPI GGATC 8 cut(s) 290, 303, 593, 768, 917, 956, 1175, 1643
BspT107I GGYRCC 2 cut(s) 136, 1601
BspTNI GGTCTC 2 cut(s) 469, 711
BsrBI CCGCTC 2 cut(s) 164, 2175
BsrDI GCAATG 1 cut(s) 242
BsrFI RCCGGY 2 cut(s) 398, 458
BsrI ACTGG 7 cut(s) 328, 356, 572, 821, 1700, 2174, 2347
BssAI RCCGGY 2 cut(s) 398, 458
BssECI CCNNGG 7 cut(s) 228, 444, 485, 1230, 1936, 2020, 2027
BssNI GRCGYC 1 cut(s) 137
BssSI CACGAG 1 cut(s) 165
BssT1I CCWWGG 3 cut(s) 228, 485, 2020
Bst2BI CACGAG 1 cut(s) 165
Bst2UI CCWGG 5 cut(s) 122, 668, 1501, 1561, 2029
Bst4CI ACNGT 5 cut(s) 85, 414, 1043, 1671, 2079
Bst6I CTCTTC 2 cut(s) 62, 205
BstACI GRCGYC 1 cut(s) 137
BstAPI GCANNNNNTGC 2 cut(s) 1300, 1712
BstC8I GCNNGC 4 cut(s) 460, 1948, 2002, 2177
BstDEI CTNAG 5 cut(s) 895, 1461, 1494, 1822, 1996
BstDSI CCRYGG 1 cut(s) 1230
BstF5I GGATG 7 cut(s) 576, 676, 734, 1351, 1627, 1652, 2360
BstFNI CGCG 6 cut(s) 106, 129, 291, 1950, 2151, 2187
BstH2I RGCGCY 2 cut(s) 140, 860
BstHHI GCGC 4 cut(s) 108, 139, 409, 859
BstMAI GTCTC 4 cut(s) 229, 469, 711, 1128
BstMCI CGRYCG 1 cut(s) 1747
BstMWI GCNNNNNNNGC 4 cut(s) 698, 775, 1300, 1712
BstNI CCWGG 5 cut(s) 122, 668, 1501, 1561, 2029
BstNSI RCATGY 1 cut(s) 1787
BstPAI GACNNNNGTC 1 cut(s) 821
BstSCI CCNGG 8 cut(s) 120, 443, 444, 666, 1499, 1559, 2027, 2363
BstSFI CTRYAG 2 cut(s) 776, 852
BstSLI GKGCMC 1 cut(s) 689
BstUI CGCG 6 cut(s) 106, 129, 291, 1950, 2151, 2187
BstV1I GCAGC 6 cut(s) 145, 866, 1118, 1290, 2219, 2413
BstV2I GAAGAC 1 cut(s) 1986
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BsuRI GGCC 9 cut(s) 5, 32, 575, 687, 1195, 1499, 1533, 1916, 2199
BtgI CCRYGG 1 cut(s) 1230
BtsCI GGATG 7 cut(s) 576, 676, 734, 1351, 1627, 1652, 2360
BtsI GCAGTG 1 cut(s) 1301
BtsIMutI CAGTG 6 cut(s) 321, 349, 426, 1301, 1593, 1667
BtuMI TCGCGA 1 cut(s) 2151
BveI ACCTGC 1 cut(s) 944
Cac8I GCNNGC 4 cut(s) 460, 1948, 2002, 2177
CfoI GCGC 4 cut(s) 108, 139, 409, 859
Cfr10I RCCGGY 2 cut(s) 398, 458
Cfr9I CCCGGG 1 cut(s) 444
CseI GACGC 1 cut(s) 297
CviAII CATG 5 cut(s) 614, 725, 1456, 1784, 1984
DdeI CTNAG 5 cut(s) 895, 1461, 1494, 1822, 1996
DinI GGCGCC 1 cut(s) 138
EaeI YGGCCR 1 cut(s) 1531
Eam1104I CTCTTC 2 cut(s) 62, 205
EarI CTCTTC 2 cut(s) 62, 205
EciI GGCGGA 2 cut(s) 167, 750
Ecl136II GAGCTC 1 cut(s) 1819
Eco130I CCWWGG 3 cut(s) 228, 485, 2020
Eco147I AGGCCT 3 cut(s) 1195, 1499, 1916
Eco24I GRGCYC 2 cut(s) 689, 1821
Eco31I GGTCTC 2 cut(s) 469, 711
Eco47I GGWCC 6 cut(s) 124, 469, 737, 933, 1361, 2024
Eco47III AGCGCT 1 cut(s) 858
Eco53kI GAGCTC 1 cut(s) 1819
Eco57I CTGAAG 2 cut(s) 1284, 1794
Eco88I CYCGRG 2 cut(s) 444, 2202
EcoICRI GAGCTC 1 cut(s) 1819
EcoO109I RGGNCCY 3 cut(s) 685, 686, 2024
EcoRII CCWGG 5 cut(s) 120, 666, 1499, 1559, 2027
EcoT14I CCWWGG 3 cut(s) 228, 485, 2020
EcoT22I ATGCAT 1 cut(s) 1789
EcoT38I GRGCYC 2 cut(s) 689, 1821
EgeI GGCGCC 1 cut(s) 138
EheI GGCGCC 1 cut(s) 138
ErhI CCWWGG 3 cut(s) 228, 485, 2020
Esp3I CGTCTC 1 cut(s) 229
FaeI CATG 5 cut(s) 617, 728, 1459, 1787, 1987
FalI AAGNNNNNCTT 2 cut(s) 1819, 1851
FatI CATG 5 cut(s) 613, 724, 1455, 1783, 1983
FauI CCCGC 3 cut(s) 284, 697, 2168
FbaI TGATCA 2 cut(s) 261, 1507
FokI GGATG 7 cut(s) 583, 683, 741, 1358, 1634, 1639, 2347
FriOI GRGCYC 2 cut(s) 689, 1821
FspBI CTAG 7 cut(s) 599, 927, 1026, 1863, 1973, 2021, 2094
FspI TGCGCA 1 cut(s) 408
GlaI GCGC 4 cut(s) 107, 138, 408, 858
GsuI CTGGAG 4 cut(s) 345, 804, 1210, 2191
HaeII RGCGCY 2 cut(s) 140, 860
HaeIII GGCC 9 cut(s) 5, 32, 575, 687, 1195, 1499, 1533, 1916, 2199
HapII CCGG 7 cut(s) 29, 399, 445, 459, 840, 1082, 2365
HgaI GACGC 1 cut(s) 297
HhaI GCGC 4 cut(s) 108, 139, 409, 859
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 5 cut(s) 617, 728, 1459, 1787, 1987
Hin6I GCGC 4 cut(s) 106, 137, 407, 857
HinP1I GCGC 4 cut(s) 106, 137, 407, 857
HindIII AAGCTT 1 cut(s) 2236
HinfI GANTC 7 cut(s) 90, 171, 772, 787, 817, 1435, 1736
HpaII CCGG 7 cut(s) 29, 399, 445, 459, 840, 1082, 2365
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 1 cut(s) 81
Hpy8I GTNNAC 1 cut(s) 81
Hpy99I CGWCG 2 cut(s) 89, 1646
HpyCH4III ACNGT 5 cut(s) 85, 414, 1043, 1671, 2079
HpyCH4IV ACGT 4 cut(s) 147, 1525, 1644, 1989
HpyF10VI GCNNNNNNNGC 4 cut(s) 698, 775, 1300, 1712
HpyF3I CTNAG 5 cut(s) 895, 1461, 1494, 1822, 1996
HpySE526I ACGT 4 cut(s) 147, 1525, 1644, 1989
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 5 cut(s) 617, 728, 1459, 1787, 1987
HspAI GCGC 4 cut(s) 106, 137, 407, 857
KasI GGCGCC 1 cut(s) 136
Kpn2I TCCGGA 2 cut(s) 839, 1081
KroI GCCGGC 1 cut(s) 458
KroNI GCCGGC 1 cut(s) 460
Ksp22I TGATCA 2 cut(s) 261, 1507
LmnI GCTCC 4 cut(s) 232, 1084, 1291, 2172
Lsp1109I GCAGC 6 cut(s) 145, 866, 1118, 1290, 2219, 2413
LweI GCATC 8 cut(s) 257, 445, 943, 1066, 1336, 1364, 1480, 1546
MaeI CTAG 7 cut(s) 599, 927, 1026, 1863, 1973, 2021, 2094
MaeII ACGT 4 cut(s) 147, 1525, 1644, 1989
MaeIII GTNAC 4 cut(s) 319, 508, 1028, 1729
MbiI CCGCTC 2 cut(s) 164, 2175
MboII GAAGA 8 cut(s) 49, 192, 373, 895, 1086, 1991, 2056, 2075
MfeI CAATTG 1 cut(s) 1350
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 2 cut(s) 689, 1821
Mly113I GGCGCC 1 cut(s) 137
MlyI GAGTC 3 cut(s) 99, 180, 811
MmeI TCCRAC 5 cut(s) 67, 118, 738, 1387, 2013
Mph1103I ATGCAT 1 cut(s) 1789
MroI TCCGGA 2 cut(s) 839, 1081
MroNI GCCGGC 1 cut(s) 458
MroXI GAANNNNTTC 2 cut(s) 635, 2398
MseI TTAA 6 cut(s) 648, 915, 1154, 1658, 2228, 2418
MslI CAYNNNNRTG 4 cut(s) 326, 1689, 1926, 2365
MspA1I CMGCKG 1 cut(s) 769
MspI CCGG 7 cut(s) 29, 399, 445, 459, 840, 1082, 2365
MspR9I CCNGG 8 cut(s) 122, 445, 446, 668, 1501, 1561, 2029, 2365
MunI CAATTG 1 cut(s) 1350
Mva1269I GAATGC 3 cut(s) 508, 989, 2095
MvaI CCWGG 5 cut(s) 122, 668, 1501, 1561, 2029
MvnI CGCG 6 cut(s) 106, 129, 291, 1950, 2151, 2187
MwoI GCNNNNNNNGC 4 cut(s) 698, 775, 1300, 1712
NaeI GCCGGC 1 cut(s) 460
NarI GGCGCC 1 cut(s) 137
NciI CCSGG 3 cut(s) 445, 446, 2365
NgoMIV GCCGGC 1 cut(s) 458
NlaIII CATG 5 cut(s) 617, 728, 1459, 1787, 1987
NlaIV GGNNCC 7 cut(s) 138, 297, 686, 687, 688, 1603, 2026
NmuCI GTSAC 2 cut(s) 319, 1028
NruI TCGCGA 1 cut(s) 2151
NsbI TGCGCA 1 cut(s) 408
NsiI ATGCAT 1 cut(s) 1789
NspI RCATGY 1 cut(s) 1787
OliI CACNNNNGTG 1 cut(s) 326
PaeR7I CTCGAG 1 cut(s) 2202
PceI AGGCCT 3 cut(s) 1195, 1499, 1916
PcsI WCGNNNNNNNCGW 2 cut(s) 1531, 2058
PctI GAATGC 3 cut(s) 508, 989, 2095
PdiI GCCGGC 1 cut(s) 460
PdmI GAANNNNTTC 2 cut(s) 635, 2398
PfeI GAWTC 4 cut(s) 772, 787, 1435, 1736
PfoI TCCNGGA 1 cut(s) 120
Ple19I CGATCG 1 cut(s) 1747
PleI GAGTC 3 cut(s) 98, 179, 811
PluTI GGCGCC 1 cut(s) 140
PpsI GAGTC 3 cut(s) 98, 179, 811
PpuMI RGGWCCY 1 cut(s) 2024
PshAI GACNNNNGTC 1 cut(s) 821
Psp124BI GAGCTC 1 cut(s) 1821
Psp5II RGGWCCY 1 cut(s) 2024
Psp6I CCWGG 5 cut(s) 120, 666, 1499, 1559, 2027
PspGI CCWGG 5 cut(s) 120, 666, 1499, 1559, 2027
PspN4I GGNNCC 7 cut(s) 138, 297, 686, 687, 688, 1603, 2026
PspOMI GGGCCC 1 cut(s) 685
PspPPI RGGWCCY 1 cut(s) 2024
PspXI VCTCGAGB 1 cut(s) 2202
PstI CTGCAG 2 cut(s) 780, 856
PsuI RGATCY 1 cut(s) 295
PvuI CGATCG 1 cut(s) 1747
PvuII CAGCTG 1 cut(s) 769
RruI TCGCGA 1 cut(s) 2151
RseI CAYNNNNRTG 4 cut(s) 326, 1689, 1926, 2365
SacI GAGCTC 1 cut(s) 1821
SaqAI TTAA 6 cut(s) 648, 915, 1154, 1658, 2228, 2418
ScaI AGTACT 5 cut(s) 1248, 1285, 2168, 2341, 2384
SchI GAGTC 3 cut(s) 99, 180, 811
ScrFI CCNGG 8 cut(s) 122, 445, 446, 668, 1501, 1561, 2029, 2365
SduI GDGCHC 2 cut(s) 689, 1821
SfaNI GCATC 8 cut(s) 257, 445, 943, 1066, 1336, 1364, 1480, 1546
SfcI CTRYAG 2 cut(s) 776, 852
SfoI GGCGCC 1 cut(s) 138
Sfr274I CTCGAG 1 cut(s) 2202
SinI GGWCC 6 cut(s) 124, 469, 737, 933, 1361, 2024
SlaI CTCGAG 1 cut(s) 2202
SmaI CCCGGG 1 cut(s) 446
SmiMI CAYNNNNRTG 4 cut(s) 326, 1689, 1926, 2365
SmlI CTYRAG 2 cut(s) 1721, 2202
SmoI CTYRAG 2 cut(s) 1721, 2202
SpeI ACTAGT 1 cut(s) 1025
SseBI AGGCCT 3 cut(s) 1195, 1499, 1916
SspDI GGCGCC 1 cut(s) 136
SspI AATATT 1 cut(s) 1514
SspMI CTAG 7 cut(s) 599, 927, 1026, 1863, 1973, 2021, 2094
SstI GAGCTC 1 cut(s) 1821
StuI AGGCCT 3 cut(s) 1195, 1499, 1916
StyD4I CCNGG 8 cut(s) 120, 443, 444, 666, 1499, 1559, 2027, 2363
StyI CCWWGG 3 cut(s) 228, 485, 2020
TaaI ACNGT 5 cut(s) 85, 414, 1043, 1671, 2079
TaiI ACGT 4 cut(s) 150, 1528, 1647, 1992
TaqI TCGA 7 cut(s) 63, 114, 210, 1743, 1747, 2118, 2203
TatI WGTACW 6 cut(s) 1086, 1246, 1283, 2166, 2339, 2382
TauI GCSGC 5 cut(s) 142, 145, 164, 183, 2253
TfiI GAWTC 4 cut(s) 772, 787, 1435, 1736
Tru1I TTAA 6 cut(s) 648, 915, 1154, 1658, 2228, 2418
Tru9I TTAA 6 cut(s) 648, 915, 1154, 1658, 2228, 2418
TscAI CASTG 6 cut(s) 328, 356, 426, 1301, 1600, 1674
TseFI GTSAC 2 cut(s) 319, 1028
TseI GCWGC 6 cut(s) 158, 854, 1106, 1303, 2207, 2426
Tsp45I GTSAC 2 cut(s) 319, 1028
TspDTI ATGAA 6 cut(s) 819, 982, 1086, 1164, 1220, 2436
TspGWI ACGGA 5 cut(s) 954, 1213, 1304, 1350, 2141
TspMI CCCGGG 1 cut(s) 444
TspRI CASTG 6 cut(s) 328, 356, 426, 1301, 1600, 1674
VpaK11BI GGWCC 6 cut(s) 124, 469, 737, 933, 1361, 2024
XapI RAATTY 5 cut(s) 11, 303, 364, 644, 718
XceI RCATGY 1 cut(s) 1787
XcmI CCANNNNNNNNNTGG 1 cut(s) 1237
XhoI CTCGAG 1 cut(s) 2202
XmaI CCCGGG 1 cut(s) 444
XmaJI CCTAGG 1 cut(s) 2020
XmnI GAANNNNTTC 2 cut(s) 635, 2398
XspI CTAG 7 cut(s) 599, 927, 1026, 1863, 1973, 2021, 2094
ZrmI AGTACT 5 cut(s) 1248, 1285, 2168, 2341, 2384
Zsp2I ATGCAT 1 cut(s) 1789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.