Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0000933

Physical Location & Seq

Genomic Coordinates Forward (+)

64199157 .. 64209740

Gene Structure 10584 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00330500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2091 bp

ATGTCGAATTTCGTAACCCAGTTGATGATTCTGATTCTTGTCTTTGTACCAGTAGCTGTTTCAGAACCACGACAACCAGTTGAGGCTTTGCTTCGTCGTCTGGACTCCAAACGCTCCTCTGCTTCAGTTCAAGAAGCTGCAGCTAAAGCTGTTCTCAAACGATTGCTGCCTACCCATGTCGACAGTTTCGACTTCAAGATTGTTGACAAGGATGTTTGTGGTGGACATAGTTGCTTTGTGATAAACAATCATAACCTGTCAAGTCGAAATGGCCCCGAGATACAGATTAAAGGTACCACAGCAGTTGAAATTGCTTCTGGCCTCCATTGGTATTTGAAGTATTGGTGTGGTGCTCATGTTTCTTGGGACAAGACTGGTGGCGTGCAATTAGTTTCCATCCCCAATCCAGGATCACTACCTCGTGTAAAAGACAAGGGGTTGAAAATTCAACGGCCGGTCCCATGGAACTATTACCAAAATGTCGTTACATCCAGTTATTCATTTGTTTGGTGGGATTGGGAAAGATGGCAGAAAGAAATAGACTGGATGGCTCTTCAGGGGATCAACCTGCCTTTAGCATTCACAGGGCAAGAGTCAATTTGGCAGAAAGTTTTCATGGATTTCAATATTAGTAAGGGAGACTTGAATGATTTCTTTGGTGGACCTGCCTTCCTTGCATGGGCTCGCATGGGGAATTTACATGCGTGGGGTGGACCATTATCACAGAATTGGTTGGATCAACAGTTGGTTTTGCAGAAACAGATATTATCCCGGATGTTAGAGCTGGGCATGACTCCAGTGCTGCCGTCATTCTCTGGAAATGTCCCAGCGACACTGAAGAAAATTTATCCTTCAGCTAATATAACCAGGCTTGGAGACTGGAACACTGTCAATGGTGATCGCCGCTGGTGCTGTACTTACCTTCTTGATCCTTCTGATCCTTTATTTGTTGAAATTGGGACTGCTTTTATTAGGCGACAAGTTGAAGAATACGGGGATGTGACAGACATCTATAACTGTGATACATTCAATGAAAATTCTCCACCTACAAATGATCCGGCTTATATTTCTTCACTTGGAGCTGCTGTATACAAAGCAATGTCCAAAGGGTTGGCTCTTCTACTCAGACTCTGGTTTTTGGAAACCACCTCAAATGAAGAATTTGTGGAGTCAACAATCTTTTCTAGATTTCTGTTTGAACGAGCTCTTTTACATTCTGTTCCATTCGGGAAAATGATTGTACTTGATTTATTTGCTGATGTGAAACCAATATGGAACACGTCATCTCAATTTTACGACACTCCCTATATCTGGTGTCTCCTGCACAATTTTGGTGGGAACCTTGAAATGTATGGGATATTGGATGCAATTTCTTCAGGTCCAGTTGATGCCCGAACTAGTGAAAATTCTACTATGGTTGGGGTTGGCATGTGCATGGAAGGAATCGAGCACAATCCAGTTATCTATGAATTGACATCTGAAATGGCATTTCGCAGTGAAAGGGTTCCAGTTCAGGATTGGCTGAGGACCTACTCCCGTAGACGTTATGGTAATGCAATTCATCAAGTTGAGGAGGCTTGGGAAATTCTTCATCGCACAATTTATAACTGCACAGATGGAATTGCTGATGTGATTAATGCTTTACGGCTATTCCTTGATGCTGGAAATGATCTTTCCGGAAGCCTTACATACAGATATGATTTGGTTGATTTAACACGCCAAGTGCTGTCAAAGCTAGCAAACCAAGTATATGTGGATGCAGTGACTGCTTTTCAGAGGAAGGATGTAAAAGCTTTTGATCTCAATAGTCAAAAGTTTGTTCAACTCATAAGGGATATTGATGCACTTCTTGCTTCTGATGATAATTTTTTACTTGGAACTTGGCTTGAAAGTGCAAAAAAGCTGGCAACCAGTCCTACGGAAATGAGGCAGTATGAGTGGAATGCAAGAACGCAGGTGACAATGTGGTATGATACCACAAAAACCAACCAGAGCCAGCTTCATGATTATGGTACAGAGCTTTACCCAGTGAAGGCAAAAGGAGATGCACTTGCTATTTCTAAAGCATTGTATGAAAAATATTTCGCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051302 GO:0051781 GO:0061458 GO:0065007

KEGG Pathways

Metabolic & Signaling

ko00531 ko01100 ko04142 map00531 map01100 map04142

Pfam Domains

Protein Families

NAGLU NAGLU_C NAGLU_N

Protein Analysis

696

Amino Acids

79.09

Weight (kDa)

5.74

Isoelectric Point (pI)

42.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAGLU_N	PF12971	46 - 140	4.3e-24	Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain
NAGLU	PF05089	157 - 498	1e-128	Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
NAGLU_C	PF12972	507 - 540	2.1e-08	Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain
NAGLU_C	PF12972	542 - 672	4e-44	Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000933)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13690 AT5G13690
fragaria_vesca	FvH4_1g18460 FvH4_1g19570 FvH4_1g19580 FvH4_5g16380 FvH4_5g16380 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470
malus_domestica	MD07G1018500.v1.1 MD07G1018700.v1.1 MD14G1187100.v1.1
prunus_persica	Prupe.2G020300_v2.0.a1 Prupe.2G020300_v2.0.a1 Prupe.2G020300_v2.0.a1 Prupe.2G020300_v2.0.a1 Prupe.5G179300_v2.0.a1 Prupe.5G179300_v2.0.a1
pyrus_communis	pycom07g01480 pycom14g15500
rosa_chinensis	RchiOBHm_Chr1g0317311 RchiOBHm_Chr7g0179731 RchiOBHm_Chr7g0179741
rosa_laevigata	RLG00000005326 RLG00000017711 RLG00000029569 RLG00000030607
rosa_multiflora	Rmu_sc0000186.1_g000002 Rmu_sc0004156.1_g000003 Rmu_sc0005092.1_g000001 Rmu_sc0013869.1_g000005 Rmu_sc0026282.1_g000001
rosa_roxburghii	Rroxscaffold_3G00273510 Rroxscaffold_4G00330500
rosa_rugosa	Rorug01G0015800 Rorug02G0157200 Rorug06G0427700
rosa_samantha	Rh1AG027200 Rh1AG093700 Rh1BG023100 Rh1CG025200 Rh1DG046000 Rh2AG208700 Rh4CG293700 Rh7AG027200 Rh7AG027300 Rh7BG026300 Rh7BG026400 Rh7CG028400 Rh7CG170000 Rh7DG028000
rosa_wichuraiana	Rw1G001880 Rw7G002190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1605
AarI	CACCTGC	1 cut(s)	1945
Acc36I	ACCTGC	3 cut(s)	576, 673, 1945
Acc65I	GGTACC	1 cut(s)	293
AccB1I	GGYRCC	1 cut(s)	293
AccI	GTMKAC	3 cut(s)	180, 1089, 1540
AccII	CGCG	1 cut(s)	2087
AccIII	TCCGGA	1 cut(s)	1676
AciI	CCGC	1 cut(s)	904
AclWI	GGATC	6 cut(s)	418, 569, 744, 923, 932, 1049
AcoI	YGGCCR	1 cut(s)	452
AcsI	RAATTY	8 cut(s)	7, 444, 694, 843, 1036, 1160, 1405, 1584
AcuI	CTGAAG	5 cut(s)	108, 539, 837, 857, 1359
AfaI	GTAC	5 cut(s)	48, 295, 916, 1242, 2014
AfiI	CCNNNNNNNGG	4 cut(s)	407, 679, 1311, 2032
AflIII	ACRYGT	1 cut(s)	1278
AhlI	ACTAGT	1 cut(s)	1397
AjiI	CACGTC	1 cut(s)	1281
AjnI	CCWGG	2 cut(s)	406, 866
Alw21I	GWGCWC	3 cut(s)	355, 1207, 1452
Alw26I	GTCTC	3 cut(s)	633, 870, 1322
AlwI	GGATC	6 cut(s)	418, 569, 744, 923, 932, 1049
AlwNI	CAGNNNCTG	3 cut(s)	56, 1131, 1766
Ama87I	CYCGRG	1 cut(s)	275
Aor13HI	TCCGGA	1 cut(s)	1676
AoxI	GGCC	3 cut(s)	271, 319, 452
ApeKI	GCWGC	5 cut(s)	137, 140, 166, 802, 1082
ApoI	RAATTY	8 cut(s)	7, 444, 694, 843, 1036, 1160, 1405, 1584
AseI	ATTAAT	1 cut(s)	1635
Asp700I	GAANNNNTTC	4 cut(s)	611, 650, 1503, 1587
Asp718I	GGTACC	1 cut(s)	293
AspS9I	GGNCC	6 cut(s)	272, 457, 662, 713, 1379, 1527
AsuC2I	CCSGG	1 cut(s)	772
AsuHPI	GGTGA	2 cut(s)	908, 1969
AsuNHI	GCTAGC	1 cut(s)	1735
AvaI	CYCGRG	1 cut(s)	275
AvaII	GGWCC	5 cut(s)	457, 662, 713, 1379, 1527
BanI	GGYRCC	1 cut(s)	293
BanII	GRGCYC	2 cut(s)	685, 1207
BauI	CACGAG	1 cut(s)	420
Bbv12I	GWGCWC	3 cut(s)	355, 1207, 1452
BbvCI	CCTCAGC	1 cut(s)	1523
BbvI	GCAGC	5 cut(s)	124, 152, 153, 789, 1069
BccI	CCATC	4 cut(s)	404, 519, 541, 1610
BceAI	ACGGC	3 cut(s)	467, 790, 1661
BciT130I	CCWGG	2 cut(s)	408, 868
BcnI	CCSGG	1 cut(s)	772
BcoDI	GTCTC	3 cut(s)	633, 870, 1322
BcuI	ACTAGT	1 cut(s)	1397
BfaI	CTAG	3 cut(s)	1185, 1398, 1736
BfmI	CTRYAG	1 cut(s)	138
BfuAI	ACCTGC	3 cut(s)	576, 673, 1945
BisI	GCNGC	6 cut(s)	138, 141, 167, 803, 904, 1083
BlsI	GCNGC	6 cut(s)	139, 142, 168, 804, 905, 1084
Bme1390I	CCNGG	3 cut(s)	408, 772, 868
Bme18I	GGWCC	5 cut(s)	457, 662, 713, 1379, 1527
BmeT110I	CYCGRG	1 cut(s)	275
BmgBI	CACGTC	1 cut(s)	1281
BmgT120I	GGNCC	6 cut(s)	272, 457, 662, 713, 1379, 1527
BmiI	GGNNCC	5 cut(s)	274, 295, 459, 1340, 1506
BmrFI	CCNGG	3 cut(s)	408, 772, 868
BmrI	ACTGGG	2 cut(s)	13, 2021
BmsI	GCATC	6 cut(s)	1354, 1378, 1648, 1747, 1831, 2035
BmtI	GCTAGC	1 cut(s)	1739
BmuI	ACTGGG	2 cut(s)	13, 2021
BpmI	CTGGAG	1 cut(s)	780
Bpu10I	CCTNAGC	1 cut(s)	1523
BpuMI	CCSGG	1 cut(s)	772
BsaJI	CCNNGG	1 cut(s)	461
BsaWI	WCCGGW	1 cut(s)	1676
BsaXI	ACNNNNNCTCC	2 cut(s)	1071, 1101
Bsc4I	CCNNNNNNNGG	4 cut(s)	407, 679, 1311, 2032
Bse118I	RCCGGY	1 cut(s)	454
Bse3DI	GCAATG	1 cut(s)	1104
BseAI	TCCGGA	1 cut(s)	1676
BseBI	CCWGG	2 cut(s)	408, 868
BseDI	CCNNGG	1 cut(s)	461
BseGI	GGATG	9 cut(s)	217, 396, 488, 552, 780, 1003, 1369, 1762, 1789
BseLI	CCNNNNNNNGG	4 cut(s)	407, 679, 1311, 2032
BseMI	GCAATG	1 cut(s)	1104
BseMII	CTCAG	2 cut(s)	1138, 1514
BseRI	GAGGAG	2 cut(s)	106, 1586
BseX3I	CGGCCG	1 cut(s)	452
BseXI	GCAGC	5 cut(s)	124, 152, 153, 789, 1069
BseYI	CCCAGC	2 cut(s)	784, 826
BsgI	GTGCAG	2 cut(s)	1307, 1594
Bsh1236I	CGCG	1 cut(s)	2087
Bsh1285I	CGRYCG	1 cut(s)	455
BshFI	GGCC	3 cut(s)	273, 321, 454
BshNI	GGYRCC	1 cut(s)	293
BsiEI	CGRYCG	1 cut(s)	455
BsiHKAI	GWGCWC	3 cut(s)	355, 1207, 1452
BsiHKCI	CYCGRG	1 cut(s)	275
BsiSI	CCGG	4 cut(s)	455, 772, 1058, 1677
BslFI	GGGAC	4 cut(s)	380, 443, 809, 973
BslI	CCNNNNNNNGG	4 cut(s)	407, 679, 1311, 2032
BsmAI	GTCTC	3 cut(s)	633, 870, 1322
BsmFI	GGGAC	4 cut(s)	380, 443, 809, 973
BsmI	GAATGC	2 cut(s)	578, 1948
BsnI	GGCC	3 cut(s)	273, 321, 454
BsoBI	CYCGRG	1 cut(s)	275
Bsp1286I	GDGCHC	4 cut(s)	355, 685, 1207, 1452
Bsp13I	TCCGGA	1 cut(s)	1676
Bsp143I	GATC	9 cut(s)	410, 561, 736, 898, 928, 937, 1054, 1669, 1798
Bsp19I	CCATGG	1 cut(s)	461
BspACI	CCGC	1 cut(s)	904
BspANI	GGCC	3 cut(s)	273, 321, 454
BspCNI	CTCAG	2 cut(s)	1137, 1515
BspEI	TCCGGA	1 cut(s)	1676
BspFNI	CGCG	1 cut(s)	2087
BspHI	TCATGA	1 cut(s)	2002
BspLI	GGNNCC	5 cut(s)	274, 295, 459, 1340, 1506
BspMAI	CTGCAG	1 cut(s)	142
BspMI	ACCTGC	3 cut(s)	576, 673, 1945
BspOI	GCTAGC	1 cut(s)	1739
BspPI	GGATC	6 cut(s)	418, 569, 744, 923, 932, 1049
BspQI	GCTCTTC	2 cut(s)	558, 1122
BspT107I	GGYRCC	1 cut(s)	293
BsrDI	GCAATG	1 cut(s)	1104
BsrFI	RCCGGY	1 cut(s)	454
BssAI	RCCGGY	1 cut(s)	454
BssECI	CCNNGG	1 cut(s)	461
BssMI	GATC	9 cut(s)	410, 561, 736, 898, 928, 937, 1054, 1669, 1798
BssNAI	GTATAC	1 cut(s)	1090
BssSI	CACGAG	1 cut(s)	420
BssT1I	CCWWGG	1 cut(s)	461
Bst1107I	GTATAC	1 cut(s)	1090
Bst2BI	CACGAG	1 cut(s)	420
Bst2UI	CCWGG	2 cut(s)	408, 868
Bst4CI	ACNGT	4 cut(s)	185, 744, 889, 1019
Bst6I	CTCTTC	2 cut(s)	558, 1122
BstAPI	GCANNNNNTGC	2 cut(s)	1766, 1850
BstC8I	GCNNGC	5 cut(s)	383, 685, 1737, 1905, 1997
BstDEI	CTNAG	2 cut(s)	1124, 1523
BstDSI	CCRYGG	1 cut(s)	461
BstF5I	GGATG	9 cut(s)	217, 396, 488, 552, 780, 1003, 1369, 1762, 1789
BstFNI	CGCG	1 cut(s)	2087
BstKTI	GATC	9 cut(s)	413, 564, 739, 901, 931, 940, 1057, 1672, 1801
BstMAI	GTCTC	3 cut(s)	633, 870, 1322
BstMBI	GATC	9 cut(s)	410, 561, 736, 898, 928, 937, 1054, 1669, 1798
BstMCI	CGRYCG	1 cut(s)	455
BstMWI	GCNNNNNNNGC	6 cut(s)	146, 674, 909, 1732, 1766, 1850
BstNI	CCWGG	2 cut(s)	408, 868
BstNSI	RCATGY	2 cut(s)	704, 1432
BstSCI	CCNGG	3 cut(s)	406, 770, 866
BstSFI	CTRYAG	1 cut(s)	138
BstUI	CGCG	1 cut(s)	2087
BstV1I	GCAGC	5 cut(s)	124, 152, 153, 789, 1069
BstXI	CCANNNNNNTGG	1 cut(s)	1111
BstZ17I	GTATAC	1 cut(s)	1090
BstZI	CGGCCG	1 cut(s)	452
BsuRI	GGCC	3 cut(s)	273, 321, 454
BtgI	CCRYGG	1 cut(s)	461
BtgZI	GCGATG	1 cut(s)	1577
BtrI	CACGTC	1 cut(s)	1281
BtsCI	GGATG	9 cut(s)	217, 396, 488, 552, 780, 1003, 1369, 1762, 1789
BtsI	GCAGTG	2 cut(s)	1501, 1767
BtsIMutI	CAGTG	6 cut(s)	804, 833, 885, 1501, 1767, 2034
BveI	ACCTGC	3 cut(s)	576, 673, 1945
Cac8I	GCNNGC	5 cut(s)	383, 685, 1737, 1905, 1997
CaiI	CAGNNNCTG	3 cut(s)	56, 1131, 1766
CciI	TCATGA	1 cut(s)	2002
Cfr10I	RCCGGY	1 cut(s)	454
Cfr13I	GGNCC	6 cut(s)	272, 457, 662, 713, 1379, 1527
Csp6I	GTAC	5 cut(s)	47, 294, 915, 1241, 2013
CspCI	CAANNNNNGTGG	6 cut(s)	286, 321, 358, 393, 1315, 1350
CviQI	GTAC	5 cut(s)	47, 294, 915, 1241, 2013
DdeI	CTNAG	2 cut(s)	1124, 1523
DpnI	GATC	9 cut(s)	412, 563, 738, 900, 930, 939, 1056, 1671, 1800
DpnII	GATC	9 cut(s)	410, 561, 736, 898, 928, 937, 1054, 1669, 1798
EaeI	YGGCCR	1 cut(s)	452
EagI	CGGCCG	1 cut(s)	452
Eam1104I	CTCTTC	2 cut(s)	558, 1122
EarI	CTCTTC	2 cut(s)	558, 1122
Ecl136II	GAGCTC	1 cut(s)	1205
EclXI	CGGCCG	1 cut(s)	452
Eco130I	CCWWGG	1 cut(s)	461
Eco24I	GRGCYC	2 cut(s)	685, 1207
Eco47I	GGWCC	5 cut(s)	457, 662, 713, 1379, 1527
Eco52I	CGGCCG	1 cut(s)	452
Eco53kI	GAGCTC	1 cut(s)	1205
Eco57I	CTGAAG	5 cut(s)	108, 539, 837, 857, 1359
Eco88I	CYCGRG	1 cut(s)	275
EcoICRI	GAGCTC	1 cut(s)	1205
EcoO109I	RGGNCCY	1 cut(s)	1527
EcoRII	CCWGG	2 cut(s)	406, 866
EcoT14I	CCWWGG	1 cut(s)	461
EcoT38I	GRGCYC	2 cut(s)	685, 1207
ErhI	CCWWGG	1 cut(s)	461
FalI	AAGNNNNNCTT	2 cut(s)	626, 658
FaqI	GGGAC	4 cut(s)	380, 443, 809, 973
FblI	GTMKAC	3 cut(s)	180, 1089, 1540
Fnu4HI	GCNGC	6 cut(s)	138, 141, 167, 803, 904, 1083
FokI	GGATG	9 cut(s)	224, 383, 475, 559, 787, 1010, 1376, 1769, 1796
FriOI	GRGCYC	2 cut(s)	685, 1207
Fsp4HI	GCNGC	6 cut(s)	138, 141, 167, 803, 904, 1083
FspBI	CTAG	3 cut(s)	1185, 1398, 1736
GluI	GCNGC	6 cut(s)	138, 141, 167, 803, 904, 1083
GsaI	CCCAGC	2 cut(s)	788, 830
GsuI	CTGGAG	1 cut(s)	780
HaeIII	GGCC	3 cut(s)	273, 321, 454
HapII	CCGG	4 cut(s)	455, 772, 1058, 1677
HincII	GTYRAC	3 cut(s)	181, 205, 1173
HindII	GTYRAC	3 cut(s)	181, 205, 1173
HindIII	AAGCTT	1 cut(s)	1791
HinfI	GANTC	8 cut(s)	28, 34, 104, 593, 793, 1128, 1169, 1443
HpaII	CCGG	4 cut(s)	455, 772, 1058, 1677
HphI	GGTGA	2 cut(s)	908, 1969
Hpy166II	GTNNAC	8 cut(s)	181, 205, 224, 662, 713, 1090, 1173, 1541
Hpy188I	TCNGA	7 cut(s)	33, 64, 937, 1127, 1480, 1776, 1858
Hpy8I	GTNNAC	8 cut(s)	181, 205, 224, 662, 713, 1090, 1173, 1541
Hpy99I	CGWCG	1 cut(s)	99
HpyAV	CCTTC	7 cut(s)	679, 861, 932, 942, 1433, 1774, 2026
HpyCH4III	ACNGT	4 cut(s)	185, 744, 889, 1019
HpyCH4IV	ACGT	2 cut(s)	1280, 1543
HpyF10VI	GCNNNNNNNGC	6 cut(s)	146, 674, 909, 1732, 1766, 1850
HpyF3I	CTNAG	2 cut(s)	1124, 1523
HpySE526I	ACGT	2 cut(s)	1280, 1543
Kpn2I	TCCGGA	1 cut(s)	1676
KpnI	GGTACC	1 cut(s)	297
Kzo9I	GATC	9 cut(s)	410, 561, 736, 898, 928, 937, 1054, 1669, 1798
LguI	GCTCTTC	2 cut(s)	558, 1122
LmnI	GCTCC	2 cut(s)	119, 1079
Lsp1109I	GCAGC	5 cut(s)	124, 152, 153, 789, 1069
LweI	GCATC	6 cut(s)	1354, 1378, 1648, 1747, 1831, 2035
MaeI	CTAG	3 cut(s)	1185, 1398, 1736
MaeII	ACGT	2 cut(s)	1280, 1543
MaeIII	GTNAC	5 cut(s)	13, 484, 1000, 1762, 1957
MalI	GATC	9 cut(s)	412, 563, 738, 900, 930, 939, 1056, 1671, 1800
MboI	GATC	9 cut(s)	410, 561, 736, 898, 928, 937, 1054, 1669, 1798
MboII	GAAGA	8 cut(s)	545, 850, 998, 1062, 1109, 1169, 1365, 1580
MhlI	GDGCHC	4 cut(s)	355, 685, 1207, 1452
MlyI	GAGTC	5 cut(s)	98, 602, 787, 1122, 1178
MmeI	TCCRAC	1 cut(s)	714
MroI	TCCGGA	1 cut(s)	1676
MroXI	GAANNNNTTC	4 cut(s)	611, 650, 1503, 1587
MseI	TTAA	3 cut(s)	288, 1635, 1712
MslI	CAYNNNNRTG	2 cut(s)	1433, 2007
MspA1I	CMGCKG	1 cut(s)	906
MspI	CCGG	4 cut(s)	455, 772, 1058, 1677
MspR9I	CCNGG	3 cut(s)	408, 772, 868
Mva1269I	GAATGC	2 cut(s)	578, 1948
MvaI	CCWGG	2 cut(s)	408, 868
MvnI	CGCG	1 cut(s)	2087
MwoI	GCNNNNNNNGC	6 cut(s)	146, 674, 909, 1732, 1766, 1850
NciI	CCSGG	1 cut(s)	772
NcoI	CCATGG	1 cut(s)	461
NdeII	GATC	9 cut(s)	410, 561, 736, 898, 928, 937, 1054, 1669, 1798
NheI	GCTAGC	1 cut(s)	1735
NlaIV	GGNNCC	5 cut(s)	274, 295, 459, 1340, 1506
NmuCI	GTSAC	3 cut(s)	1000, 1762, 1957
NspI	RCATGY	2 cut(s)	704, 1432
PagI	TCATGA	1 cut(s)	2002
PaqCI	CACCTGC	1 cut(s)	1945
PciSI	GCTCTTC	2 cut(s)	558, 1122
PcsI	WCGNNNNNNNCGW	1 cut(s)	186
PctI	GAATGC	2 cut(s)	578, 1948
PdmI	GAANNNNTTC	4 cut(s)	611, 650, 1503, 1587
PfeI	GAWTC	3 cut(s)	28, 34, 1443
PfoI	TCCNGGA	2 cut(s)	406, 770
PkrI	GCNGC	6 cut(s)	139, 142, 168, 804, 905, 1084
PleI	GAGTC	5 cut(s)	98, 601, 787, 1122, 1177
PpsI	GAGTC	5 cut(s)	98, 601, 787, 1122, 1177
PpuMI	RGGWCCY	1 cut(s)	1527
PshBI	ATTAAT	1 cut(s)	1635
PsiI	TTATAA	1 cut(s)	1605
Psp124BI	GAGCTC	1 cut(s)	1207
Psp5II	RGGWCCY	1 cut(s)	1527
Psp6I	CCWGG	2 cut(s)	406, 866
PspFI	CCCAGC	2 cut(s)	784, 826
PspGI	CCWGG	2 cut(s)	406, 866
PspN4I	GGNNCC	5 cut(s)	274, 295, 459, 1340, 1506
PspPI	GGNCC	6 cut(s)	272, 457, 662, 713, 1379, 1527
PspPPI	RGGWCCY	1 cut(s)	1527
PstI	CTGCAG	1 cut(s)	142
PstNI	CAGNNNCTG	3 cut(s)	56, 1131, 1766
RsaI	GTAC	5 cut(s)	48, 295, 916, 1242, 2014
RsaNI	GTAC	5 cut(s)	47, 294, 915, 1241, 2013
RseI	CAYNNNNRTG	2 cut(s)	1433, 2007
SacI	GAGCTC	1 cut(s)	1207
SalI	GTCGAC	1 cut(s)	179
SapI	GCTCTTC	2 cut(s)	558, 1122
SaqAI	TTAA	3 cut(s)	288, 1635, 1712
SatI	GCNGC	6 cut(s)	138, 141, 167, 803, 904, 1083
Sau3AI	GATC	9 cut(s)	410, 561, 736, 898, 928, 937, 1054, 1669, 1798
Sau96I	GGNCC	6 cut(s)	272, 457, 662, 713, 1379, 1527
SchI	GAGTC	5 cut(s)	98, 602, 787, 1122, 1178
ScrFI	CCNGG	3 cut(s)	408, 772, 868
SduI	GDGCHC	4 cut(s)	355, 685, 1207, 1452
SfaNI	GCATC	6 cut(s)	1354, 1378, 1648, 1747, 1831, 2035
SfcI	CTRYAG	1 cut(s)	138
SinI	GGWCC	5 cut(s)	457, 662, 713, 1379, 1527
SmiMI	CAYNNNNRTG	2 cut(s)	1433, 2007
SpeI	ACTAGT	1 cut(s)	1397
SsiI	CCGC	1 cut(s)	904
SspI	AATATT	2 cut(s)	628, 2081
SspMI	CTAG	3 cut(s)	1185, 1398, 1736
SstI	GAGCTC	1 cut(s)	1207
StyD4I	CCNGG	3 cut(s)	406, 770, 866
StyI	CCWWGG	1 cut(s)	461
TaaI	ACNGT	4 cut(s)	185, 744, 889, 1019
TaiI	ACGT	2 cut(s)	1283, 1546
TaqI	TCGA	5 cut(s)	5, 180, 189, 265, 1446
TatI	WGTACW	2 cut(s)	914, 1240
TauI	GCSGC	1 cut(s)	906
TfiI	GAWTC	3 cut(s)	28, 34, 1443
Tru1I	TTAA	3 cut(s)	288, 1635, 1712
Tru9I	TTAA	3 cut(s)	288, 1635, 1712
TscAI	CASTG	6 cut(s)	804, 840, 892, 1501, 1767, 2034
TseFI	GTSAC	3 cut(s)	1000, 1762, 1957
TseI	GCWGC	5 cut(s)	137, 140, 166, 802, 1082
Tsp45I	GTSAC	3 cut(s)	1000, 1762, 1957
TspDTI	ATGAA	9 cut(s)	489, 604, 1047, 1170, 1482, 1550, 1580, 1991, 2088
TspGWI	ACGGA	1 cut(s)	1934
TspRI	CASTG	6 cut(s)	804, 840, 892, 1501, 1767, 2034
VpaK11BI	GGWCC	5 cut(s)	457, 662, 713, 1379, 1527
VspI	ATTAAT	1 cut(s)	1635
XapI	RAATTY	8 cut(s)	7, 444, 694, 843, 1036, 1160, 1405, 1584
XbaI	TCTAGA	1 cut(s)	1184
XceI	RCATGY	2 cut(s)	704, 1432
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1515
XmiI	GTMKAC	3 cut(s)	180, 1089, 1540
XmnI	GAANNNNTTC	4 cut(s)	611, 650, 1503, 1587
XspI	CTAG	3 cut(s)	1185, 1398, 1736

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.