Rorug06G0427700

alpha-N-acetylglucosaminidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000933
Physical Location & Seq
Forward (+)
56426977 .. 56428269
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0427700.1

Sequence Viewer

Length: 1293 bp
ATGGCTCAACTGAATCTTGCAATTGTTGGACATGTCGATTCGGGAAAATCGACACTTTCAGGTAGATTGCTTCATCTATTGGGAAGGATATCCAAAAAAGAAATGCACAAAAATGAGAAAGAGGCAAAGTCACAGGGCAAGGGGTCATTTGCATATGCGTGGGCATTGGATGAGAGCGTAGAAGAAAGAGAGAGAGGAATAACTATGAGTGTAGCTGTTGCATATTTTGACTCCAAAAAATACAAGGTTGTTGTGCTGGACTCTCCTGGCCACAGAGACTTTGTCCCGAACATGATCTCCGGCGCAACACAAGCCGATGCTGCAGTACTTGTTGTTGATGGTTCACATGGTGCATTTGAAGCCGGTATGAAGGGAGGACAGACTAAGGAGCATGCACAGATCATCAAAAGCTTCGGCGTCAAGCAGATTATAGTTGCTGTTAACAAGATGGACGTTGTTGGCTACTCCAAGGATAGATTTGATGAAATCAAACACACACTAGGAACATTTCTCTGGCGTTCCTGTCGTTTCGAAAATTCTTCGGTGTGTTGGGTTCCATTGAGTGCCATGGAGAATCAGAACTTGGTGTCAGCTCCTTCTGATGACCGTTTGTCTTGGTATAGCGGACTTTGCTTCTTGGATGCAGTTGATTCCCTTCAACCTCCGAAGAGAGAGTCTGAGAAGTCACTGCTCATGCCTATATGTGATGTCATTAAATCTTCTAAAGGGCAGGTTTCTGCTTGTGGTAAATTGCAAGCTGGAGCTCTTCAAAATGGACTTAAGGTTCGGGTTATGCCTTTGGGGGAATTAGGGACTGTCCGGTCTTTAGAACGTGACGGCTCTCAGTCATGCTCAACTGCCACAGCTGGTGACAATGTGGCTGTTACTCTACAAGGGATTGATGATCGTCATGTGATGGCAGGAGGCGTGCTATGTGACCCTGATGTTCCAGTTGCATTTGCGAAACATTTGGAATTGAATGTGCAACTTTTGGACGGGGCAAAGCCTATGTTGGTTGGCTCTCAGCTGGAGTTTCACATACACCACGCAAAGGAGGCAGCAAGAGTCGTGAAAATAGCTTCATTACTTGATCCGAAGACTCTTAAAGTGGCGAAAAGGTTTCCTCGCTGTCTTCTCGCAAAGCAGGTAGCTGTTATAGAGGTAGTTCTGCAAGGGCCAGTTTGTGTGGTGGAGTACTCCATGTGTAGAGCTCTAGGGAACGTTTCTTTGAGAGCGTTAGGAAACACTGTGGCCCTCGGCAAGGTAACACGAATAATCGAGAAGCAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
NAGLU NAGLU_C NAGLU_N

Protein Analysis

430

Amino Acids

46.54

Weight (kDa)

8.95

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 4 - 219 2.8e-49 Elongation factor Tu GTP binding domain
GTP-eEF1A_C PF22594 324 - 423 1.3e-22 GTP-eEF1A C-terminal domain-like
GTP_EFTU_D3 PF03143 335 - 425 1.6e-06 Elongation factor Tu C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 820
Acc36I ACCTGC 2 cut(s) 721, 1135
AciI CCGC 1 cut(s) 624
AclI AACGTT 1 cut(s) 1221
AclWI GGATC 1 cut(s) 1085
AcoI YGGCCR 1 cut(s) 268
AcsI RAATTY 1 cut(s) 535
AcyI GRCGYC 1 cut(s) 417
AdeI CACNNNGTG 1 cut(s) 350
AfaI GTAC 2 cut(s) 327, 1196
AfiI CCNNNNNNNGG 2 cut(s) 1051, 1261
AflII CTTAAG 1 cut(s) 779
AflIII ACRYGT 1 cut(s) 31
AgsI TTSAA 4 cut(s) 359, 659, 770, 979
AhdI GACNNNNNGTC 1 cut(s) 610
AjnI CCWGG 1 cut(s) 265
AjuI GAANNNNNNNTTGG 2 cut(s) 566, 598
AloI GAACNNNNNNTCC 4 cut(s) 281, 313, 768, 800
Alw21I GWGCWC 2 cut(s) 766, 1213
Alw26I GTCTC 1 cut(s) 270
AlwI GGATC 1 cut(s) 1085
AoxI GGCC 3 cut(s) 268, 1175, 1251
ApeKI GCWGC 2 cut(s) 320, 1058
ApoI RAATTY 1 cut(s) 535
ArsI GACNNNNNNTTYG 4 cut(s) 659, 691, 768, 800
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 2 cut(s) 1175, 1252
AsuHPI GGTGA 1 cut(s) 881
AsuII TTCGAA 1 cut(s) 531
BalI TGGCCA 1 cut(s) 270
BanII GRGCYC 2 cut(s) 766, 1213
BbsI GAAGAC 2 cut(s) 1103, 1124
Bbv12I GWGCWC 2 cut(s) 766, 1213
BbvI GCAGC 2 cut(s) 307, 1070
BccI CCATC 3 cut(s) 332, 442, 910
BceAI ACGGC 1 cut(s) 853
BciT130I CCWGG 1 cut(s) 267
BcoDI GTCTC 1 cut(s) 270
BfaI CTAG 2 cut(s) 500, 1214
BfmI CTRYAG 1 cut(s) 321
BfrI CTTAAG 1 cut(s) 779
BfuAI ACCTGC 2 cut(s) 721, 1135
BisI GCNGC 2 cut(s) 321, 1059
BlsI GCNGC 2 cut(s) 322, 1060
BmcAI AGTACT 2 cut(s) 327, 1196
Bme1390I CCNGG 1 cut(s) 267
BmeRI GACNNNNNGTC 1 cut(s) 610
BmgT120I GGNCC 2 cut(s) 1175, 1252
BmiI GGNNCC 1 cut(s) 555
BmrFI CCNGG 1 cut(s) 267
BmsI GCATC 2 cut(s) 307, 631
BpiI GAAGAC 2 cut(s) 1103, 1124
BpmI CTGGAG 2 cut(s) 780, 1049
Bpu14I TTCGAA 1 cut(s) 531
BsaHI GRCGYC 1 cut(s) 417
BsaJI CCNNGG 3 cut(s) 468, 567, 1255
BsaWI WCCGGW 1 cut(s) 819
BsaXI ACNNNNNCTCC 2 cut(s) 281, 311
Bsc4I CCNNNNNNNGG 2 cut(s) 1051, 1261
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 2 cut(s) 950, 1178
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 3 cut(s) 468, 567, 1255
BseGI GGATG 2 cut(s) 175, 646
BseLI CCNNNNNNNGG 2 cut(s) 1051, 1261
BseMII CTCAG 3 cut(s) 669, 857, 1037
BseNI ACTGG 2 cut(s) 950, 1178
BseXI GCAGC 2 cut(s) 307, 1070
BshFI GGCC 3 cut(s) 270, 1177, 1253
BsiHKAI GWGCWC 2 cut(s) 766, 1213
BsiSI CCGG 3 cut(s) 300, 363, 820
BslFI GGGAC 2 cut(s) 269, 826
BslI CCNNNNNNNGG 2 cut(s) 1051, 1261
BsmAI GTCTC 1 cut(s) 270
BsmFI GGGAC 2 cut(s) 269, 826
BsnI GGCC 3 cut(s) 270, 1177, 1253
Bsp119I TTCGAA 1 cut(s) 531
Bsp1286I GDGCHC 2 cut(s) 766, 1213
Bsp143I GATC 4 cut(s) 294, 399, 904, 1090
Bsp19I CCATGG 1 cut(s) 567
BspACI CCGC 1 cut(s) 624
BspANI GGCC 3 cut(s) 270, 1177, 1253
BspCNI CTCAG 3 cut(s) 670, 856, 1036
BspLI GGNNCC 1 cut(s) 555
BspMAI CTGCAG 1 cut(s) 325
BspMI ACCTGC 2 cut(s) 721, 1135
BspPI GGATC 1 cut(s) 1085
BspQI GCTCTTC 1 cut(s) 771
BspT104I TTCGAA 1 cut(s) 531
BspTI CTTAAG 1 cut(s) 779
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 2 cut(s) 950, 1178
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 3 cut(s) 468, 567, 1255
BssMI GATC 4 cut(s) 294, 399, 904, 1090
BssNI GRCGYC 1 cut(s) 417
BssT1I CCWWGG 2 cut(s) 468, 567
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 3 cut(s) 608, 817, 1249
Bst6I CTCTTC 2 cut(s) 662, 771
BstACI GRCGYC 1 cut(s) 417
BstAFI CTTAAG 1 cut(s) 779
BstBI TTCGAA 1 cut(s) 531
BstC8I GCNNGC 3 cut(s) 393, 756, 929
BstDEI CTNAG 4 cut(s) 384, 678, 843, 1023
BstDSI CCRYGG 1 cut(s) 567
BstENI CCTNNNNNAGG 1 cut(s) 1259
BstF5I GGATG 2 cut(s) 175, 646
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 4 cut(s) 297, 402, 907, 1093
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 4 cut(s) 294, 399, 904, 1090
BstMWI GCNNNNNNNGC 5 cut(s) 311, 320, 359, 630, 1055
BstNI CCWGG 1 cut(s) 267
BstNSI RCATGY 2 cut(s) 35, 395
BstSCI CCNGG 1 cut(s) 265
BstSFI CTRYAG 1 cut(s) 321
BstV1I GCAGC 2 cut(s) 307, 1070
BstV2I GAAGAC 2 cut(s) 1103, 1124
BsuRI GGCC 3 cut(s) 270, 1177, 1253
BtgI CCRYGG 1 cut(s) 567
BtsCI GGATG 2 cut(s) 175, 646
BtsI GCAGTG 1 cut(s) 686
BtsIMutI CAGTG 2 cut(s) 686, 1245
BveI ACCTGC 2 cut(s) 721, 1135
Cac8I GCNNGC 3 cut(s) 393, 756, 929
CfoI GCGC 1 cut(s) 305
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 2 cut(s) 1175, 1252
CseI GACGC 1 cut(s) 406
Csp6I GTAC 2 cut(s) 326, 1195
CviAII CATG 9 cut(s) 32, 292, 347, 392, 568, 694, 849, 911, 1201
CviQI GTAC 2 cut(s) 326, 1195
DdeI CTNAG 4 cut(s) 384, 678, 843, 1023
DpnI GATC 4 cut(s) 296, 401, 906, 1092
DpnII GATC 4 cut(s) 294, 399, 904, 1090
DraIII CACNNNGTG 1 cut(s) 350
DrdI GACNNNNNNGTC 1 cut(s) 820
DriI GACNNNNNGTC 1 cut(s) 610
DseDI GACNNNNNNGTC 1 cut(s) 820
EaeI YGGCCR 1 cut(s) 268
Eam1104I CTCTTC 2 cut(s) 662, 771
Eam1105I GACNNNNNGTC 1 cut(s) 610
EarI CTCTTC 2 cut(s) 662, 771
Ecl136II GAGCTC 2 cut(s) 764, 1211
Eco130I CCWWGG 2 cut(s) 468, 567
Eco24I GRGCYC 2 cut(s) 766, 1213
Eco32I GATATC 1 cut(s) 90
Eco53kI GAGCTC 2 cut(s) 764, 1211
EcoICRI GAGCTC 2 cut(s) 764, 1211
EcoNI CCTNNNNNAGG 1 cut(s) 1259
EcoRII CCWGG 1 cut(s) 265
EcoRV GATATC 1 cut(s) 90
EcoT14I CCWWGG 2 cut(s) 468, 567
EcoT38I GRGCYC 2 cut(s) 766, 1213
ErhI CCWWGG 2 cut(s) 468, 567
FaeI CATG 9 cut(s) 35, 295, 350, 395, 571, 697, 852, 914, 1204
FaqI GGGAC 2 cut(s) 269, 826
FatI CATG 9 cut(s) 31, 291, 346, 391, 567, 693, 848, 910, 1200
FauNDI CATATG 1 cut(s) 154
Fnu4HI GCNGC 2 cut(s) 321, 1059
FokI GGATG 2 cut(s) 182, 653
FriOI GRGCYC 2 cut(s) 766, 1213
Fsp4HI GCNGC 2 cut(s) 321, 1059
FspBI CTAG 2 cut(s) 500, 1214
GlaI GCGC 1 cut(s) 304
GluI GCNGC 2 cut(s) 321, 1059
GsuI CTGGAG 2 cut(s) 780, 1049
HaeIII GGCC 3 cut(s) 270, 1177, 1253
HapII CCGG 3 cut(s) 300, 363, 820
HgaI GACGC 1 cut(s) 406
HhaI GCGC 1 cut(s) 305
Hin1I GRCGYC 1 cut(s) 417
Hin1II CATG 9 cut(s) 35, 295, 350, 395, 571, 697, 852, 914, 1204
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HindIII AAGCTT 1 cut(s) 409
HinfI GANTC 9 cut(s) 13, 38, 230, 260, 574, 650, 674, 1065, 1099
HpaI GTTAAC 1 cut(s) 442
HpaII CCGG 3 cut(s) 300, 363, 820
HphI GGTGA 1 cut(s) 881
Hpy166II GTNNAC 2 cut(s) 344, 442
Hpy188I TCNGA 5 cut(s) 579, 601, 666, 679, 1095
Hpy188III TCNNGA 4 cut(s) 42, 286, 1069, 1279
Hpy8I GTNNAC 2 cut(s) 344, 442
HpyAV CCTTC 4 cut(s) 78, 364, 606, 665
HpyCH4III ACNGT 3 cut(s) 608, 817, 1249
HpyCH4IV ACGT 3 cut(s) 453, 832, 1221
HpyF10VI GCNNNNNNNGC 5 cut(s) 311, 320, 359, 630, 1055
HpyF3I CTNAG 4 cut(s) 384, 678, 843, 1023
HpySE526I ACGT 3 cut(s) 453, 832, 1221
Hsp92I GRCGYC 1 cut(s) 417
Hsp92II CATG 9 cut(s) 35, 295, 350, 395, 571, 697, 852, 914, 1204
HspAI GCGC 1 cut(s) 303
KspAI GTTAAC 1 cut(s) 442
Kzo9I GATC 4 cut(s) 294, 399, 904, 1090
LguI GCTCTTC 1 cut(s) 771
LmnI GCTCC 3 cut(s) 388, 598, 761
Lsp1109I GCAGC 2 cut(s) 307, 1070
LweI GCATC 2 cut(s) 307, 631
MaeI CTAG 2 cut(s) 500, 1214
MaeII ACGT 3 cut(s) 453, 832, 1221
MaeIII GTNAC 7 cut(s) 129, 684, 833, 869, 883, 935, 1264
MalI GATC 4 cut(s) 296, 401, 906, 1092
MboI GATC 4 cut(s) 294, 399, 904, 1090
MboII GAAGA 7 cut(s) 194, 531, 679, 711, 758, 1108, 1124
MfeI CAATTG 1 cut(s) 21
MhlI GDGCHC 2 cut(s) 766, 1213
MlsI TGGCCA 1 cut(s) 270
MluCI AATT 5 cut(s) 21, 535, 749, 806, 974
MluNI TGGCCA 1 cut(s) 270
MlyI GAGTC 5 cut(s) 224, 254, 683, 1074, 1093
MmeI TCCRAC 1 cut(s) 7
MnlI CCTC 9 cut(s) 115, 188, 368, 672, 917, 1048, 1134, 1153, 1265
Mox20I TGGCCA 1 cut(s) 270
MscI TGGCCA 1 cut(s) 270
MseI TTAA 4 cut(s) 441, 714, 780, 1104
MslI CAYNNNNRTG 2 cut(s) 111, 157
Msp20I TGGCCA 1 cut(s) 270
MspA1I CMGCKG 2 cut(s) 866, 1027
MspCI CTTAAG 1 cut(s) 779
MspI CCGG 3 cut(s) 300, 363, 820
MspR9I CCNGG 1 cut(s) 267
MunI CAATTG 1 cut(s) 21
MvaI CCWGG 1 cut(s) 267
MwoI GCNNNNNNNGC 5 cut(s) 311, 320, 359, 630, 1055
NcoI CCATGG 1 cut(s) 567
NdeI CATATG 1 cut(s) 154
NdeII GATC 4 cut(s) 294, 399, 904, 1090
NlaIII CATG 9 cut(s) 35, 295, 350, 395, 571, 697, 852, 914, 1204
NlaIV GGNNCC 1 cut(s) 555
NmeAIII GCCGAG 1 cut(s) 1236
NmuCI GTSAC 5 cut(s) 129, 684, 833, 869, 935
NspI RCATGY 2 cut(s) 35, 395
NspV TTCGAA 1 cut(s) 531
PaeI GCATGC 1 cut(s) 395
PciI ACATGT 1 cut(s) 31
PciSI GCTCTTC 1 cut(s) 771
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PfeI GAWTC 4 cut(s) 13, 38, 574, 650
PflFI GACNNNGTC 1 cut(s) 281
PkrI GCNGC 2 cut(s) 322, 1060
PleI GAGTC 5 cut(s) 224, 254, 682, 1073, 1093
PpsI GAGTC 5 cut(s) 224, 254, 682, 1073, 1093
PscI ACATGT 1 cut(s) 31
Psp124BI GAGCTC 2 cut(s) 766, 1213
Psp1406I AACGTT 1 cut(s) 1221
Psp6I CCWGG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 265
PspN4I GGNNCC 1 cut(s) 555
PspPI GGNCC 2 cut(s) 1175, 1252
PstI CTGCAG 1 cut(s) 325
PsyI GACNNNGTC 1 cut(s) 281
PvuII CAGCTG 2 cut(s) 866, 1027
RsaI GTAC 2 cut(s) 327, 1196
RsaNI GTAC 2 cut(s) 326, 1195
RseI CAYNNNNRTG 2 cut(s) 111, 157
SacI GAGCTC 2 cut(s) 766, 1213
SapI GCTCTTC 1 cut(s) 771
SaqAI TTAA 4 cut(s) 441, 714, 780, 1104
SatI GCNGC 2 cut(s) 321, 1059
Sau3AI GATC 4 cut(s) 294, 399, 904, 1090
Sau96I GGNCC 2 cut(s) 1175, 1252
ScaI AGTACT 2 cut(s) 327, 1196
SchI GAGTC 5 cut(s) 224, 254, 683, 1074, 1093
ScrFI CCNGG 1 cut(s) 267
SduI GDGCHC 2 cut(s) 766, 1213
SfaNI GCATC 2 cut(s) 307, 631
SfcI CTRYAG 1 cut(s) 321
SfuI TTCGAA 1 cut(s) 531
SmiMI CAYNNNNRTG 2 cut(s) 111, 157
SmlI CTYRAG 1 cut(s) 779
SmoI CTYRAG 1 cut(s) 779
SphI GCATGC 1 cut(s) 395
Sse9I AATT 5 cut(s) 21, 535, 749, 806, 974
SsiI CCGC 1 cut(s) 624
SspMI CTAG 2 cut(s) 500, 1214
SstI GAGCTC 2 cut(s) 766, 1213
StyD4I CCNGG 1 cut(s) 265
StyI CCWWGG 2 cut(s) 468, 567
TaaI ACNGT 3 cut(s) 608, 817, 1249
TaiI ACGT 3 cut(s) 456, 835, 1224
TaqI TCGA 4 cut(s) 36, 50, 531, 1278
TasI AATT 5 cut(s) 21, 535, 749, 806, 974
TatI WGTACW 2 cut(s) 325, 1194
TfiI GAWTC 4 cut(s) 13, 38, 574, 650
Tru1I TTAA 4 cut(s) 441, 714, 780, 1104
Tru9I TTAA 4 cut(s) 441, 714, 780, 1104
TscAI CASTG 2 cut(s) 693, 1252
TseFI GTSAC 5 cut(s) 129, 684, 833, 869, 935
TseI GCWGC 2 cut(s) 320, 1058
Tsp45I GTSAC 5 cut(s) 129, 684, 833, 869, 935
TspDTI ATGAA 4 cut(s) 62, 383, 498, 1071
TspRI CASTG 2 cut(s) 693, 1252
Tth111I GACNNNGTC 1 cut(s) 281
Vha464I CTTAAG 1 cut(s) 779
XagI CCTNNNNNAGG 1 cut(s) 1259
XapI RAATTY 1 cut(s) 535
XceI RCATGY 2 cut(s) 35, 395
XspI CTAG 2 cut(s) 500, 1214
ZrmI AGTACT 2 cut(s) 327, 1196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.