Rroxscaffold_3G00273510

alpha-N-acetylglucosaminidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000933
Physical Location & Seq
Reverse (-)
65258255 .. 65264173
5919 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00273510.1

Sequence Viewer

Length: 2205 bp
ATGATTAAGAACCATCCTTCTTTTCGTAGACGAGGGGATCCACAGATTCTGATTTCCGGAGTCACTGGAGTGGAGATTTTGGCTGGTCTACATTGGTATTTGAAGAATTGGTGTGGCTCACATATATCTTGGGATAAAACTGGTGGTGCGCAACTATTTTCAGTCCCAAAAGCAGGGTTTCTCTCTCGTGTAGGAGATGGTGGCATTTTAGTCCAGAGACCTGTTCCTTGGAACTATTACCAGAATGCTGTCACATCTAGCTATTCTTTGGCTTGGTGGGACTGGAATAGATGGGAGAAGGAGATTGATATGATGGCCCTCCAGGGTATCAATTTGCCACTAGCATTTACAGGTCAAGAGGCTATATGGCAGAAAGTTTTCCAGAAATTTAATATGAGCAAATCAGATTTGGATGATTTCTTTGGAGGCCCTGCATTCCTTGCATGGTCTCGAATGGGAAATTTGCATGGATGGGGTGGACCTCTCCCGCAAAGTTGGCTTGATCAACAGCTGATTCTGCAGAAAAAAATTCTCGACAGAATGTATGAACTTGGAATGACTCCAGTCCTTCCAGCATTTTCTGGGAATGTTCCTGCAGCACTCAAAACTACATATCCATCAGCAAAGATTATACAGTTAGGAAACTGGTTTTCTGTTAGGAGTGACCCTAGATGGACCTGCACTTACCTTCTTGATGCAACTGATCCCTTATTTGTTGAAATTGGGAAAACATTCATTGAGGAACAACTAAAAGAATATGGAAGGACCAGTCACATTTACAACTGTGATACTTTTGATGAGAATACTCCACCTGTTGATGATCCAGAGTACATCTCTGCATTAGGTAAAACAATATTTAAGGGACTGCAAAGTGGAGATAAGGACGGTGTCTGGCTAATGCAGGGATGGTTATTTTCATATGATCCGTTCTGGAGACCTGCACAGATGAAGGCACTCTTACATTCTGTTCCTGCAGGGAGGATGGTAGTCCTTGATCTGTTTGCTGAAGTGAAACCAATATGGACTACTTCAGAGCAGTTTTATGGTGTTCCTTACATCTGGTGTATGCTGCACAACTTTGCAGGAAACGTTGAGATGTACGGCGTTATGGATGCAATTGCATCTGGACCAATTGATGCTTGGACAAGTGAAAACTCAACCATGGTTGGGGTTGGAATGTCAATGGAAGGTATAGAACAGAACCCTGTTGTTTATGATCTCATGTCTGAGATGGCTTTTCAACAGAACAAAGTTGATGTTAAGGACTGGATTAATCTGTATTCAACGAGACGTTATGGTCGTGCAGTTCCTTTAGTGCAACATGCCTGGAGTATCTTACATCATACCATCTATAATTGCACTGATGGTGCCTACGACAAAAATAGGGATGTGATTGTGGCATTTCCGGACGTTGATCCATCCTTTATAGCAAGACCGTCTCAAGGTTATTACCAAAATGATAAACCATTAGTATCAAGAAGATCAGTCCTGAAGGAAATAACTGATTCATTTGAGCGACCTCATTTATGGTACTCGACTTCTGAAGTAGTACATGCATTAGAACTTTTTATTGCAAGTGGCGATGAACTCTCAGAAAGCAACACTTACAGGTATGACCTTGTTGACCTGACTAGACAAGCTTTGGCTAAGTATGCAAATCAGCTGTTTCTGAAGGTCATAGAAGCCTATCATTTAAATGATATCCTTGAAGTAGTTGGTCTCAGCCAGAAGTTTTTGGAACTGGTTGAAGATATGGACACACTCTTAGCATGCCATGATGGATTTCTTCTTGGACCATGGTTGGAAAGCGCAAAGAAACTCGCCCAAGACAAAGAGCAGGAAATACAGTTTGAATGGAATGCTAGAACTCAAATAACTATGTGGTTTGACAACACAGAAGAGGAAGCAAGTCTTCTTCGCGACTATGGAAACAAGTATTGGAGTGGGCTTCTGCGAGACTACTATGGTCCCCGAGCAGCCATATACTTCAAGTATCTGATAAAAAGCTTGGACGAGGGTAATGATTTTGACTTGAAGAACTGGAGGAGGGAGTGGATCAAGCTCACAAATGACTGGCAAAACAGCAGGAGCACATTCCCAGTGAAAAGCACCGGAAATGCGGTGACTACTTCTCGGTTGCTTTTTGATAAGTACTTGCGAAGCGCTGACATAGAATCACTGGATGAAGCTGCTATTATGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
NAGLU NAGLU_C NAGLU_N

Protein Analysis

734

Amino Acids

84.28

Weight (kDa)

5.21

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAGLU_N PF12971 11 - 52 4.2e-11 Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain
NAGLU PF05089 79 - 414 3.1e-143 Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
NAGLU_C PF12972 423 - 717 3e-87 Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1296
Acc16I TGCGCA 1 cut(s) 150
Acc36I ACCTGC 2 cut(s) 686, 946
AccB1I GGYRCC 1 cut(s) 1367
AccI GTMKAC 2 cut(s) 28, 88
AccII CGCG 1 cut(s) 1920
AccIII TCCGGA 2 cut(s) 56, 1405
AciI CCGC 2 cut(s) 488, 2120
AclI AACGTT 1 cut(s) 1089
AclWI GGATC 7 cut(s) 32, 45, 698, 815, 917, 1409, 2063
AcsI RAATTY 3 cut(s) 386, 460, 528
AcuI CTGAAG 5 cut(s) 1014, 1026, 1511, 1563, 1691
AfaI GTAC 5 cut(s) 830, 1100, 1532, 1551, 2153
AfeI AGCGCT 1 cut(s) 2164
AfiI CCNNNNNNNGG 3 cut(s) 173, 1167, 1442
AgsI TTSAA 9 cut(s) 103, 719, 1241, 1284, 1709, 1748, 1853, 1990, 2035
AjnI CCWGG 2 cut(s) 321, 1325
AjuI GAANNNNNNNTTGG 2 cut(s) 1921, 1953
AleI CACNNNNGTG 1 cut(s) 68
AluBI AGCT 7 cut(s) 261, 511, 1640, 1663, 2007, 2062, 2189
AluI AGCT 7 cut(s) 261, 511, 1640, 1663, 2007, 2062, 2189
Alw21I GWGCWC 1 cut(s) 2093
Alw26I GTCTC 7 cut(s) 211, 453, 928, 1282, 1443, 1724, 1950
AlwI GGATC 7 cut(s) 32, 45, 698, 815, 917, 1409, 2063
AlwNI CAGNNNCTG 1 cut(s) 49
Ama87I CYCGRG 1 cut(s) 1971
Aor13HI TCCGGA 2 cut(s) 56, 1405
Aor51HI AGCGCT 1 cut(s) 2164
AoxI GGCC 2 cut(s) 315, 427
ApeKI GCWGC 4 cut(s) 596, 1069, 1976, 2189
ApoI RAATTY 3 cut(s) 386, 460, 528
AseI ATTAAT 1 cut(s) 1272
Asp700I GAANNNNTTC 2 cut(s) 377, 731
AspLEI GCGC 3 cut(s) 151, 1811, 2165
AspS9I GGNCC 8 cut(s) 316, 428, 479, 675, 765, 1127, 1793, 1967
AsuHPI GGTGA 1 cut(s) 2134
AvaI CYCGRG 1 cut(s) 1971
AvaII GGWCC 6 cut(s) 479, 675, 765, 1127, 1793, 1967
BamHI GGATCC 1 cut(s) 37
BanI GGYRCC 1 cut(s) 1367
BauI CACGAG 1 cut(s) 186
BbsI GAAGAC 1 cut(s) 1904
Bbv12I GWGCWC 1 cut(s) 2093
BbvI GCAGC 4 cut(s) 608, 1056, 1988, 2176
BceAI ACGGC 1 cut(s) 1117
BciT130I CCWGG 2 cut(s) 323, 1327
BclI TGATCA 1 cut(s) 502
BcoDI GTCTC 7 cut(s) 211, 453, 928, 1282, 1443, 1724, 1950
BfaI CTAG 5 cut(s) 258, 341, 669, 1632, 1863
BfmI CTRYAG 3 cut(s) 518, 594, 972
BfoI RGCGCY 1 cut(s) 2166
BfuAI ACCTGC 2 cut(s) 686, 946
BisI GCNGC 4 cut(s) 597, 1070, 1977, 2190
BlsI GCNGC 4 cut(s) 598, 1071, 1978, 2191
BmcAI AGTACT 1 cut(s) 2153
Bme1390I CCNGG 2 cut(s) 323, 1327
Bme18I GGWCC 6 cut(s) 479, 675, 765, 1127, 1793, 1967
BmeT110I CYCGRG 1 cut(s) 1971
BmgT120I GGNCC 8 cut(s) 316, 428, 479, 675, 765, 1127, 1793, 1967
BmiI GGNNCC 3 cut(s) 39, 1369, 1969
BmrFI CCNGG 2 cut(s) 323, 1327
BmrI ACTGGG 1 cut(s) 2093
BmsI GCATC 4 cut(s) 685, 1102, 1126, 1130
BmuI ACTGGG 1 cut(s) 2093
BoxI GACNNNNGTC 1 cut(s) 563
BpiI GAAGAC 1 cut(s) 1904
BplI GAGNNNNNCTC 2 cut(s) 818, 850
BpmI CTGGAG 6 cut(s) 87, 305, 546, 952, 1348, 2062
BpuEI CTTGAG 1 cut(s) 1425
BsaI GGTCTC 4 cut(s) 211, 453, 928, 1724
BsaJI CCNNGG 4 cut(s) 227, 322, 1161, 1796
BsaWI WCCGGW 3 cut(s) 56, 1405, 2111
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 1167, 1442
BseAI TCCGGA 2 cut(s) 56, 1405
BseBI CCWGG 2 cut(s) 323, 1327
BseDI CCNNGG 4 cut(s) 227, 322, 1161, 1796
BseGI GGATG 9 cut(s) 13, 418, 476, 911, 987, 1117, 1393, 1418, 2188
BseLI CCNNNNNNNGG 3 cut(s) 173, 1167, 1442
BseMII CTCAG 3 cut(s) 1218, 1605, 1735
BseRI GAGGAG 1 cut(s) 2059
BseXI GCAGC 4 cut(s) 608, 1056, 1988, 2176
BsgI GTGCAG 4 cut(s) 664, 924, 1055, 1323
Bsh1236I CGCG 1 cut(s) 1920
BshFI GGCC 2 cut(s) 317, 429
BshNI GGYRCC 1 cut(s) 1367
BsiHKAI GWGCWC 1 cut(s) 2093
BsiHKCI CYCGRG 1 cut(s) 1971
BsiSI CCGG 3 cut(s) 57, 1406, 2112
BslFI GGGAC 4 cut(s) 149, 293, 876, 1953
BslI CCNNNNNNNGG 3 cut(s) 173, 1167, 1442
BsmAI GTCTC 7 cut(s) 211, 453, 928, 1282, 1443, 1724, 1950
BsmBI CGTCTC 2 cut(s) 1282, 1443
BsmFI GGGAC 4 cut(s) 149, 293, 876, 1953
BsmI GAATGC 3 cut(s) 250, 434, 1864
BsnI GGCC 2 cut(s) 317, 429
Bso31I GGTCTC 4 cut(s) 211, 453, 928, 1724
BsoBI CYCGRG 1 cut(s) 1971
Bsp1286I GDGCHC 1 cut(s) 2093
Bsp13I TCCGGA 2 cut(s) 56, 1405
Bsp19I CCATGG 2 cut(s) 1161, 1796
Bsp68I TCGCGA 1 cut(s) 1920
BspACI CCGC 2 cut(s) 488, 2120
BspANI GGCC 2 cut(s) 317, 429
BspCNI CTCAG 3 cut(s) 1219, 1604, 1734
BspEI TCCGGA 2 cut(s) 56, 1405
BspFNI CGCG 1 cut(s) 1920
BspLI GGNNCC 3 cut(s) 39, 1369, 1969
BspMAI CTGCAG 3 cut(s) 522, 598, 976
BspMI ACCTGC 2 cut(s) 686, 946
BspPI GGATC 7 cut(s) 32, 45, 698, 815, 917, 1409, 2063
BspT107I GGYRCC 1 cut(s) 1367
BspTNI GGTCTC 4 cut(s) 211, 453, 928, 1724
BssECI CCNNGG 4 cut(s) 227, 322, 1161, 1796
BssSI CACGAG 1 cut(s) 186
BssT1I CCWWGG 3 cut(s) 227, 1161, 1796
Bst2BI CACGAG 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 323, 1327
Bst4CI ACNGT 5 cut(s) 636, 785, 887, 1437, 1848
Bst6I CTCTTC 1 cut(s) 1893
BstAPI GCANNNNNTGC 1 cut(s) 440
BstC8I GCNNGC 1 cut(s) 1771
BstDEI CTNAG 5 cut(s) 1227, 1591, 1647, 1721, 1765
BstDSI CCRYGG 2 cut(s) 1161, 1796
BstF5I GGATG 9 cut(s) 13, 418, 476, 911, 987, 1117, 1393, 1418, 2188
BstFNI CGCG 1 cut(s) 1920
BstH2I RGCGCY 1 cut(s) 2166
BstHHI GCGC 3 cut(s) 151, 1811, 2165
BstMAI GTCTC 7 cut(s) 211, 453, 928, 1282, 1443, 1724, 1950
BstMWI GCNNNNNNNGC 4 cut(s) 440, 496, 517, 1652
BstNI CCWGG 2 cut(s) 323, 1327
BstNSI RCATGY 3 cut(s) 1325, 1556, 1773
BstPAI GACNNNNGTC 1 cut(s) 563
BstSCI CCNGG 2 cut(s) 321, 1325
BstSFI CTRYAG 3 cut(s) 518, 594, 972
BstUI CGCG 1 cut(s) 1920
BstV1I GCAGC 4 cut(s) 608, 1056, 1988, 2176
BstV2I GAAGAC 1 cut(s) 1904
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuRI GGCC 2 cut(s) 317, 429
BtgI CCRYGG 2 cut(s) 1161, 1796
BtgZI GCGATG 1 cut(s) 1596
BtsCI GGATG 9 cut(s) 13, 418, 476, 911, 987, 1117, 1393, 1418, 2188
BtsIMutI CAGTG 4 cut(s) 63, 1359, 2106, 2177
BtuMI TCGCGA 1 cut(s) 1920
BveI ACCTGC 2 cut(s) 686, 946
Cac8I GCNNGC 1 cut(s) 1771
CaiI CAGNNNCTG 1 cut(s) 49
CfoI GCGC 3 cut(s) 151, 1811, 2165
Cfr13I GGNCC 8 cut(s) 316, 428, 479, 675, 765, 1127, 1793, 1967
Csp6I GTAC 5 cut(s) 829, 1099, 1531, 1550, 2152
CviAII CATG 9 cut(s) 444, 467, 1162, 1222, 1322, 1553, 1770, 1775, 1797
CviQI GTAC 5 cut(s) 829, 1099, 1531, 1550, 2152
DdeI CTNAG 5 cut(s) 1227, 1591, 1647, 1721, 1765
DraI TTTAAA 1 cut(s) 1695
DrdI GACNNNNNNGTC 1 cut(s) 1296
DseDI GACNNNNNNGTC 1 cut(s) 1296
Eam1104I CTCTTC 1 cut(s) 1893
EarI CTCTTC 1 cut(s) 1893
Eco130I CCWWGG 3 cut(s) 227, 1161, 1796
Eco31I GGTCTC 4 cut(s) 211, 453, 928, 1724
Eco32I GATATC 1 cut(s) 1702
Eco47I GGWCC 6 cut(s) 479, 675, 765, 1127, 1793, 1967
Eco47III AGCGCT 1 cut(s) 2164
Eco57I CTGAAG 5 cut(s) 1014, 1026, 1511, 1563, 1691
Eco88I CYCGRG 1 cut(s) 1971
EcoO109I RGGNCCY 1 cut(s) 428
EcoRII CCWGG 2 cut(s) 321, 1325
EcoRV GATATC 1 cut(s) 1702
EcoT14I CCWWGG 3 cut(s) 227, 1161, 1796
EcoT22I ATGCAT 1 cut(s) 1558
ErhI CCWWGG 3 cut(s) 227, 1161, 1796
Esp3I CGTCTC 2 cut(s) 1282, 1443
FaeI CATG 9 cut(s) 447, 470, 1165, 1225, 1325, 1556, 1773, 1778, 1800
FalI AAGNNNNNCTT 6 cut(s) 941, 973, 1588, 1620, 1896, 1928
FaqI GGGAC 4 cut(s) 149, 293, 876, 1953
FatI CATG 9 cut(s) 443, 466, 1161, 1221, 1321, 1552, 1769, 1774, 1796
FauI CCCGC 1 cut(s) 495
FauNDI CATATG 1 cut(s) 919
FbaI TGATCA 1 cut(s) 502
FblI GTMKAC 2 cut(s) 28, 88
Fnu4HI GCNGC 4 cut(s) 597, 1070, 1977, 2190
FokI GGATG 8 cut(s) 425, 483, 918, 994, 1124, 1400, 1405, 2195
Fsp4HI GCNGC 4 cut(s) 597, 1070, 1977, 2190
FspBI CTAG 5 cut(s) 258, 341, 669, 1632, 1863
FspI TGCGCA 1 cut(s) 150
GlaI GCGC 3 cut(s) 150, 1810, 2164
GluI GCNGC 4 cut(s) 597, 1070, 1977, 2190
GsuI CTGGAG 6 cut(s) 87, 305, 546, 952, 1348, 2062
HaeII RGCGCY 1 cut(s) 2166
HaeIII GGCC 2 cut(s) 317, 429
HapII CCGG 3 cut(s) 57, 1406, 2112
HhaI GCGC 3 cut(s) 151, 1811, 2165
Hin1II CATG 9 cut(s) 447, 470, 1165, 1225, 1325, 1556, 1773, 1778, 1800
Hin6I GCGC 3 cut(s) 149, 1809, 2163
HinP1I GCGC 3 cut(s) 149, 1809, 2163
HincII GTYRAC 1 cut(s) 1624
HindII GTYRAC 1 cut(s) 1624
HindIII AAGCTT 2 cut(s) 1638, 2005
HinfI GANTC 6 cut(s) 46, 60, 514, 559, 1505, 2174
HpaII CCGG 3 cut(s) 57, 1406, 2112
HphI GGTGA 1 cut(s) 2134
Hpy166II GTNNAC 4 cut(s) 29, 89, 479, 1624
Hpy188I TCNGA 8 cut(s) 51, 406, 1033, 1228, 1543, 1594, 1671, 1998
Hpy8I GTNNAC 4 cut(s) 29, 89, 479, 1624
HpyAV CCTTC 9 cut(s) 27, 292, 578, 698, 756, 943, 1181, 1486, 1666
HpyCH4III ACNGT 5 cut(s) 636, 785, 887, 1437, 1848
HpyCH4IV ACGT 3 cut(s) 1089, 1291, 1410
HpyF10VI GCNNNNNNNGC 4 cut(s) 440, 496, 517, 1652
HpyF3I CTNAG 5 cut(s) 1227, 1591, 1647, 1721, 1765
HpySE526I ACGT 3 cut(s) 1089, 1291, 1410
Hsp92II CATG 9 cut(s) 447, 470, 1165, 1225, 1325, 1556, 1773, 1778, 1800
HspAI GCGC 3 cut(s) 149, 1809, 2163
Kpn2I TCCGGA 2 cut(s) 56, 1405
Ksp22I TGATCA 1 cut(s) 502
LmnI GCTCC 1 cut(s) 2088
Lsp1109I GCAGC 4 cut(s) 608, 1056, 1988, 2176
LweI GCATC 4 cut(s) 685, 1102, 1126, 1130
MaeI CTAG 5 cut(s) 258, 341, 669, 1632, 1863
MaeII ACGT 3 cut(s) 1089, 1291, 1410
MaeIII GTNAC 5 cut(s) 61, 250, 662, 770, 2122
MboII GAAGA 8 cut(s) 115, 1491, 1760, 1778, 1904, 1907, 1910, 2047
MfeI CAATTG 2 cut(s) 1116, 1131
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 2093
MluCI AATT 9 cut(s) 106, 331, 386, 460, 528, 720, 1116, 1131, 1354
MlyI GAGTC 2 cut(s) 69, 553
MmeI TCCRAC 2 cut(s) 1153, 1782
Mph1103I ATGCAT 1 cut(s) 1558
MroI TCCGGA 2 cut(s) 56, 1405
MroXI GAANNNNTTC 2 cut(s) 377, 731
MseI TTAA 6 cut(s) 6, 390, 858, 1260, 1272, 1694
MslI CAYNNNNRTG 2 cut(s) 68, 1695
MspA1I CMGCKG 2 cut(s) 511, 1663
MspI CCGG 3 cut(s) 57, 1406, 2112
MspR9I CCNGG 2 cut(s) 323, 1327
MunI CAATTG 2 cut(s) 1116, 1131
Mva1269I GAATGC 3 cut(s) 250, 434, 1864
MvaI CCWGG 2 cut(s) 323, 1327
MvnI CGCG 1 cut(s) 1920
MwoI GCNNNNNNNGC 4 cut(s) 440, 496, 517, 1652
NcoI CCATGG 2 cut(s) 1161, 1796
NdeI CATATG 1 cut(s) 919
NlaIII CATG 9 cut(s) 447, 470, 1165, 1225, 1325, 1556, 1773, 1778, 1800
NlaIV GGNNCC 3 cut(s) 39, 1369, 1969
NmuCI GTSAC 5 cut(s) 61, 250, 662, 770, 2122
NruI TCGCGA 1 cut(s) 1920
NsbI TGCGCA 1 cut(s) 150
NsiI ATGCAT 1 cut(s) 1558
NspI RCATGY 3 cut(s) 1325, 1556, 1773
OliI CACNNNNGTG 1 cut(s) 68
PaeI GCATGC 1 cut(s) 1773
PcsI WCGNNNNNNNCGW 1 cut(s) 1297
PctI GAATGC 3 cut(s) 250, 434, 1864
PdmI GAANNNNTTC 2 cut(s) 377, 731
PfeI GAWTC 4 cut(s) 46, 514, 1505, 2174
PflFI GACNNNGTC 1 cut(s) 887
PkrI GCNGC 4 cut(s) 598, 1071, 1978, 2191
PleI GAGTC 2 cut(s) 68, 553
PpsI GAGTC 2 cut(s) 68, 553
PshAI GACNNNNGTC 1 cut(s) 563
PshBI ATTAAT 1 cut(s) 1272
Psp1406I AACGTT 1 cut(s) 1089
Psp6I CCWGG 2 cut(s) 321, 1325
PspGI CCWGG 2 cut(s) 321, 1325
PspN4I GGNNCC 3 cut(s) 39, 1369, 1969
PspPI GGNCC 8 cut(s) 316, 428, 479, 675, 765, 1127, 1793, 1967
PstI CTGCAG 3 cut(s) 522, 598, 976
PstNI CAGNNNCTG 1 cut(s) 49
PsuI RGATCY 1 cut(s) 37
PsyI GACNNNGTC 1 cut(s) 887
PvuII CAGCTG 2 cut(s) 511, 1663
RruI TCGCGA 1 cut(s) 1920
RsaI GTAC 5 cut(s) 830, 1100, 1532, 1551, 2153
RsaNI GTAC 5 cut(s) 829, 1099, 1531, 1550, 2152
RseI CAYNNNNRTG 2 cut(s) 68, 1695
SaqAI TTAA 6 cut(s) 6, 390, 858, 1260, 1272, 1694
SatI GCNGC 4 cut(s) 597, 1070, 1977, 2190
Sau96I GGNCC 8 cut(s) 316, 428, 479, 675, 765, 1127, 1793, 1967
SbfI CCTGCAGG 1 cut(s) 976
ScaI AGTACT 1 cut(s) 2153
SchI GAGTC 2 cut(s) 69, 553
ScrFI CCNGG 2 cut(s) 323, 1327
SdaI CCTGCAGG 1 cut(s) 976
SduI GDGCHC 1 cut(s) 2093
SfaNI GCATC 4 cut(s) 685, 1102, 1126, 1130
SfcI CTRYAG 3 cut(s) 518, 594, 972
SinI GGWCC 6 cut(s) 479, 675, 765, 1127, 1793, 1967
SmiI ATTTAAAT 1 cut(s) 1695
SmiMI CAYNNNNRTG 2 cut(s) 68, 1695
SmlI CTYRAG 1 cut(s) 1440
SmoI CTYRAG 1 cut(s) 1440
SphI GCATGC 1 cut(s) 1773
Sse8387I CCTGCAGG 1 cut(s) 976
Sse9I AATT 9 cut(s) 106, 331, 386, 460, 528, 720, 1116, 1131, 1354
SsiI CCGC 2 cut(s) 488, 2120
SspI AATATT 1 cut(s) 855
SspMI CTAG 5 cut(s) 258, 341, 669, 1632, 1863
StyD4I CCNGG 2 cut(s) 321, 1325
StyI CCWWGG 3 cut(s) 227, 1161, 1796
SwaI ATTTAAAT 1 cut(s) 1695
TaaI ACNGT 5 cut(s) 636, 785, 887, 1437, 1848
TaiI ACGT 3 cut(s) 1092, 1294, 1413
TaqI TCGA 3 cut(s) 451, 534, 1535
TasI AATT 9 cut(s) 106, 331, 386, 460, 528, 720, 1116, 1131, 1354
TatI WGTACW 3 cut(s) 828, 1549, 2151
TfiI GAWTC 4 cut(s) 46, 514, 1505, 2174
Tru1I TTAA 6 cut(s) 6, 390, 858, 1260, 1272, 1694
Tru9I TTAA 6 cut(s) 6, 390, 858, 1260, 1272, 1694
TscAI CASTG 4 cut(s) 70, 1366, 2106, 2184
TseFI GTSAC 5 cut(s) 61, 250, 662, 770, 2122
TseI GCWGC 4 cut(s) 596, 1069, 1976, 2189
Tsp45I GTSAC 5 cut(s) 61, 250, 662, 770, 2122
TspDTI ATGAA 7 cut(s) 561, 724, 906, 962, 1497, 1599, 2199
TspGWI ACGGA 1 cut(s) 915
TspRI CASTG 4 cut(s) 70, 1366, 2106, 2184
Tth111I GACNNNGTC 1 cut(s) 887
VpaK11BI GGWCC 6 cut(s) 479, 675, 765, 1127, 1793, 1967
VspI ATTAAT 1 cut(s) 1272
XapI RAATTY 3 cut(s) 386, 460, 528
XceI RCATGY 3 cut(s) 1325, 1556, 1773
XcmI CCANNNNNNNNNTGG 1 cut(s) 1137
XmiI GTMKAC 2 cut(s) 28, 88
XmnI GAANNNNTTC 2 cut(s) 377, 731
XspI CTAG 5 cut(s) 258, 341, 669, 1632, 1863
ZrmI AGTACT 1 cut(s) 2153
Zsp2I ATGCAT 1 cut(s) 1558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.