Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1B

Orthogroup ID

OG0000933

Physical Location & Seq

Genomic Coordinates Reverse (-)

3038844 .. 3048805

Gene Structure 9962 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1BG023100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1830 bp

ATGTCGAATTCCATAACCCAGTTGATGTTTCTGATTCTTGTCTTTGTACCAATAGCTGTATCATCAAAACCACAACAACCAGTTGAGGCTTTGCTGCGTCGTCTGGACTCCAAACGCTCCTCTGCTTCAGTTCAAGAAGCTGCAGCTAAAGCTGTTCTCTTTAGATTGCTTCCTACCCATGTCGACAGTTTCGACTTCAAGATTGTTGACAAGGATGTTTGTGGTGGACATAGTTGCTTTGTGATAAACAATCACAACCTGTCAAGTCGAAATGGCCCCGAGATACAGATTAAAGGTACCACAGCAGTTGAAATTGCTTCTGGCCTCCATTGGTATTTGAAGTATTGGTGTGGTGCTCATGTTTCTTGGGATAAGACTGGTGGCGTGCAATTAGCTTCCATCCCCAATCCAGGATCACTACCTCGTGTAAAAGACAAGGGGTTGAAAATTCAACGGCCGGTCCCATGGAACTATTACCAAAATGTCGTTACATCCAGTTATTCATTTGTTTGGTGGGATTGGGAAAGATGGCAGAAAGAAATAGACTGGATGGCTCTTCAGGGGATCAACCTGCCTTTAGCATTCACAGGGCAAGAATCAATTTGGCAGAAAGTTTTCATGGTGGGGTGGACCATTATCACAGAATTGGTTGGATCAACAATTGGTTTTGCAGAAACAGATATTATCCCGGATGTTAGAGCTAGGCATGACTCCAGGTGTCTCCTGCACAATTTTGGTGGGAACCTTGAAATGTATGGGATATTGGATGCAATTTCTTCAGGTCCAGTTGATGCCCGAACTAGTGAAAATTCTACTATGGTTGGGGTTGGCATGTGCATGGAAGGAATCGAGCACAATCCAGTTATCTATGAATTGACGTCTGAAATGGCATTTCGCAGTGAAAGGGTTCCAGTTCAGGATTGGCTGAGGACCTACTCCCGTAGACGTTATGGTAATGCAGTTCATCAAGTTGAGGAGGCTTGGGAAATTCTTCATCGCACAATTTATAACTGCACAGATGGAATTGCTGACCATAACACAGATTTCATAGTCAAATTACCTGACTGGGATCCATCACTAGAGAGTGTATCGAACACGTCAGAAACTAACAAGATGCAAATGTTTTTCACACTAGATATGAAGAGGAGATTTTTACTCCAAAAAACCAGCTCTCAATTTCCTAAGGCACATTTGTGGTATTCAACTCAGGATGTGATTAATGCTTTACGGCTATTCCTTGATGCTGGAAATGATCTTTCCAGAAGCCTTACGTACAGATATGATTTGGTTGATTTAACACGCCAAGTGCTGTCAAAGCTAGCAAACCAAGTATATGTGAATGCAGTGACTGCTTTTCAGAGGAAGGATGTAAAAGCTTTTGATCTCAATAGTAAAAAGTTTGTTCAACTCATAAGGGATATTGATGTACTTCTTGCTTCTGATGATAATTTTTTACTTGGAACTTGGCTTGAAAGTGCAAAAAAGCTGGCAACCAGTCCTACGGAAATGAGGCAGTATGAGTGGAATGCAAGAACACAGGTGACAATGTGGTATGATACCACAAAAACCAACCAGAGCCAGCTTCATGATTATGCAAATAAGTTCTGGAGTGGCCTATTGGAAAGTTACTATCTACCACGAGCTTCAACCTACTTTCATTATTTGTCAAAAAGCTTAAGAGAAAATAAAGACTTTGAAGTGGAAAAATGGAGAATAGAATGGATATCCTTTTCAAACAATTGGCAAGCAGGTACAGAGCTTTACCCAGTGAAGGCAAAAGGAGATGCACTTGCTATTTCTAAAGCATTGTATGAAAAATATTTCGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051302 GO:0051781 GO:0061458 GO:0065007

KEGG Pathways

Metabolic & Signaling

ko00531 ko01100 ko04142 map00531 map01100 map04142

Pfam Domains

Protein Families

NAGLU NAGLU_C NAGLU_N

Protein Analysis

609

Amino Acids

70.04

Weight (kDa)

6.93

Isoelectric Point (pI)

40.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAGLU_N	PF12971	47 - 141	2.7e-23	Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain
NAGLU	PF05089	158 - 207	2.1e-22	Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
NAGLU	PF05089	240 - 299	2.2e-17	Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
NAGLU_C	PF12972	308 - 606	8.4e-91	Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000933)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13690 AT5G13690
fragaria_vesca	FvH4_1g18460 FvH4_1g19570 FvH4_1g19580 FvH4_5g16380 FvH4_5g16380 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470 FvH4_7g01470
malus_domestica	MD07G1018500.v1.1 MD07G1018700.v1.1 MD14G1187100.v1.1
prunus_persica	Prupe.2G020300_v2.0.a1 Prupe.2G020300_v2.0.a1 Prupe.2G020300_v2.0.a1 Prupe.2G020300_v2.0.a1 Prupe.5G179300_v2.0.a1 Prupe.5G179300_v2.0.a1
pyrus_communis	pycom07g01480 pycom14g15500
rosa_chinensis	RchiOBHm_Chr1g0317311 RchiOBHm_Chr7g0179731 RchiOBHm_Chr7g0179741
rosa_laevigata	RLG00000005326 RLG00000017711 RLG00000029569 RLG00000030607
rosa_multiflora	Rmu_sc0000186.1_g000002 Rmu_sc0004156.1_g000003 Rmu_sc0005092.1_g000001 Rmu_sc0013869.1_g000005 Rmu_sc0026282.1_g000001
rosa_roxburghii	Rroxscaffold_3G00273510 Rroxscaffold_4G00330500
rosa_rugosa	Rorug01G0015800 Rorug02G0157200 Rorug06G0427700
rosa_samantha	Rh1AG027200 Rh1AG093700 Rh1BG023100 Rh1CG025200 Rh1DG046000 Rh2AG208700 Rh4CG293700 Rh7AG027200 Rh7AG027300 Rh7BG026300 Rh7BG026400 Rh7CG028400 Rh7CG170000 Rh7DG028000
rosa_wichuraiana	Rw1G001880 Rw7G002190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1008
AatII	GACGTC	1 cut(s)	881
Acc36I	ACCTGC	2 cut(s)	579, 1739
Acc65I	GGTACC	1 cut(s)	296
AccB1I	GGYRCC	1 cut(s)	296
AccI	GTMKAC	2 cut(s)	183, 943
AclWI	GGATC	5 cut(s)	421, 572, 661, 1064, 1077
AcoI	YGGCCR	1 cut(s)	455
AcsI	RAATTY	4 cut(s)	7, 447, 808, 987
AcuI	CTGAAG	3 cut(s)	111, 542, 762
AcyI	GRCGYC	1 cut(s)	878
AfaI	GTAC	5 cut(s)	48, 298, 1274, 1428, 1753
AfiI	CCNNNNNNNGG	2 cut(s)	410, 1771
AflII	CTTAAG	1 cut(s)	1675
AflIII	ACRYGT	1 cut(s)	1095
AhlI	ACTAGT	1 cut(s)	800
AjiI	CACGTC	1 cut(s)	1098
AjnI	CCWGG	2 cut(s)	409, 713
AleI	CACNNNNGTG	1 cut(s)	1192
Alw21I	GWGCWC	2 cut(s)	358, 855
Alw26I	GTCTC	1 cut(s)	725
AlwI	GGATC	5 cut(s)	421, 572, 661, 1064, 1077
AlwNI	CAGNNNCTG	1 cut(s)	1349
Ama87I	CYCGRG	1 cut(s)	278
AoxI	GGCC	4 cut(s)	274, 322, 455, 1612
ApeKI	GCWGC	3 cut(s)	94, 140, 143
ApoI	RAATTY	4 cut(s)	7, 447, 808, 987
AseI	ATTAAT	1 cut(s)	1218
Asp700I	GAANNNNTTC	3 cut(s)	614, 906, 990
Asp718I	GGTACC	1 cut(s)	296
AspS9I	GGNCC	5 cut(s)	275, 460, 630, 782, 930
AsuC2I	CCSGG	1 cut(s)	689
AsuHPI	GGTGA	1 cut(s)	1552
AsuNHI	GCTAGC	1 cut(s)	1318
AvaI	CYCGRG	1 cut(s)	278
AvaII	GGWCC	4 cut(s)	460, 630, 782, 930
AxyI	CCTNAGG	1 cut(s)	1182
BamHI	GGATCC	1 cut(s)	1069
BanI	GGYRCC	1 cut(s)	296
BauI	CACGAG	2 cut(s)	423, 1638
Bbv12I	GWGCWC	2 cut(s)	358, 855
BbvCI	CCTCAGC	1 cut(s)	926
BbvI	GCAGC	3 cut(s)	81, 127, 155
BccI	CCATC	5 cut(s)	407, 522, 544, 1013, 1081
BceAI	ACGGC	2 cut(s)	470, 1244
BciT130I	CCWGG	2 cut(s)	411, 715
BcnI	CCSGG	1 cut(s)	689
BcoDI	GTCTC	1 cut(s)	725
BcuI	ACTAGT	1 cut(s)	800
BfaI	CTAG	5 cut(s)	702, 801, 1079, 1133, 1319
BfmI	CTRYAG	1 cut(s)	141
BfrI	CTTAAG	1 cut(s)	1675
BfuAI	ACCTGC	2 cut(s)	579, 1739
BisI	GCNGC	3 cut(s)	95, 141, 144
BlsI	GCNGC	3 cut(s)	96, 142, 145
Bme1390I	CCNGG	3 cut(s)	411, 689, 715
Bme18I	GGWCC	4 cut(s)	460, 630, 782, 930
BmeT110I	CYCGRG	1 cut(s)	278
BmgBI	CACGTC	1 cut(s)	1098
BmgT120I	GGNCC	5 cut(s)	275, 460, 630, 782, 930
BmiI	GGNNCC	6 cut(s)	277, 298, 462, 743, 909, 1071
BmrFI	CCNGG	3 cut(s)	411, 689, 715
BmrI	ACTGGG	3 cut(s)	13, 1075, 1760
BmsI	GCATC	5 cut(s)	757, 781, 1104, 1231, 1774
BmtI	GCTAGC	1 cut(s)	1322
BmuI	ACTGGG	3 cut(s)	13, 1075, 1760
BpmI	CTGGAG	2 cut(s)	697, 1627
Bpu10I	CCTNAGC	1 cut(s)	926
BpuMI	CCSGG	1 cut(s)	689
BsaAI	YACGTR	1 cut(s)	1272
BsaHI	GRCGYC	1 cut(s)	878
BsaJI	CCNNGG	1 cut(s)	464
Bsc4I	CCNNNNNNNGG	2 cut(s)	410, 1771
Bse118I	RCCGGY	1 cut(s)	457
Bse21I	CCTNAGG	1 cut(s)	1182
BseBI	CCWGG	2 cut(s)	411, 715
BseDI	CCNNGG	1 cut(s)	464
BseGI	GGATG	8 cut(s)	220, 399, 491, 555, 697, 772, 1216, 1372
BseLI	CCNNNNNNNGG	2 cut(s)	410, 1771
BseMII	CTCAG	2 cut(s)	917, 1220
BseRI	GAGGAG	3 cut(s)	109, 989, 1159
BseX3I	CGGCCG	1 cut(s)	455
BseXI	GCAGC	3 cut(s)	81, 127, 155
BsgI	GTGCAG	2 cut(s)	710, 997
Bsh1285I	CGRYCG	1 cut(s)	458
BshFI	GGCC	4 cut(s)	276, 324, 457, 1614
BshNI	GGYRCC	1 cut(s)	296
BsiEI	CGRYCG	1 cut(s)	458
BsiHKAI	GWGCWC	2 cut(s)	358, 855
BsiHKCI	CYCGRG	1 cut(s)	278
BsiSI	CCGG	2 cut(s)	458, 689
BslFI	GGGAC	1 cut(s)	446
BslI	CCNNNNNNNGG	2 cut(s)	410, 1771
BsmAI	GTCTC	1 cut(s)	725
BsmFI	GGGAC	1 cut(s)	446
BsmI	GAATGC	3 cut(s)	581, 1345, 1531
BsnI	GGCC	4 cut(s)	276, 324, 457, 1614
BsoBI	CYCGRG	1 cut(s)	278
Bsp1286I	GDGCHC	2 cut(s)	358, 855
Bsp143I	GATC	6 cut(s)	413, 564, 653, 1069, 1252, 1381
Bsp19I	CCATGG	1 cut(s)	464
BspANI	GGCC	4 cut(s)	276, 324, 457, 1614
BspCNI	CTCAG	2 cut(s)	918, 1219
BspHI	TCATGA	1 cut(s)	1585
BspLI	GGNNCC	6 cut(s)	277, 298, 462, 743, 909, 1071
BspMAI	CTGCAG	1 cut(s)	145
BspMI	ACCTGC	2 cut(s)	579, 1739
BspOI	GCTAGC	1 cut(s)	1322
BspPI	GGATC	5 cut(s)	421, 572, 661, 1064, 1077
BspQI	GCTCTTC	1 cut(s)	561
BspT107I	GGYRCC	1 cut(s)	296
BspTI	CTTAAG	1 cut(s)	1675
BsrFI	RCCGGY	1 cut(s)	457
BssAI	RCCGGY	1 cut(s)	457
BssECI	CCNNGG	1 cut(s)	464
BssMI	GATC	6 cut(s)	413, 564, 653, 1069, 1252, 1381
BssNI	GRCGYC	1 cut(s)	878
BssSI	CACGAG	2 cut(s)	423, 1638
BssT1I	CCWWGG	1 cut(s)	464
Bst2BI	CACGAG	2 cut(s)	423, 1638
Bst2UI	CCWGG	2 cut(s)	411, 715
Bst4CI	ACNGT	1 cut(s)	188
Bst6I	CTCTTC	2 cut(s)	561, 1136
BstACI	GRCGYC	1 cut(s)	878
BstAFI	CTTAAG	1 cut(s)	1675
BstAPI	GCANNNNNTGC	1 cut(s)	1349
BstBAI	YACGTR	1 cut(s)	1272
BstC8I	GCNNGC	5 cut(s)	386, 1320, 1488, 1580, 1746
BstDEI	CTNAG	3 cut(s)	926, 1182, 1206
BstDSI	CCRYGG	1 cut(s)	464
BstF5I	GGATG	8 cut(s)	220, 399, 491, 555, 697, 772, 1216, 1372
BstKTI	GATC	6 cut(s)	416, 567, 656, 1072, 1255, 1384
BstMAI	GTCTC	1 cut(s)	725
BstMBI	GATC	6 cut(s)	413, 564, 653, 1069, 1252, 1381
BstMCI	CGRYCG	1 cut(s)	458
BstMWI	GCNNNNNNNGC	3 cut(s)	149, 1315, 1349
BstNI	CCWGG	2 cut(s)	411, 715
BstNSI	RCATGY	1 cut(s)	835
BstSCI	CCNGG	3 cut(s)	409, 687, 713
BstSFI	CTRYAG	1 cut(s)	141
BstSNI	TACGTA	1 cut(s)	1272
BstV1I	GCAGC	3 cut(s)	81, 127, 155
BstX2I	RGATCY	1 cut(s)	1069
BstYI	RGATCY	1 cut(s)	1069
BstZI	CGGCCG	1 cut(s)	455
Bsu36I	CCTNAGG	1 cut(s)	1182
BsuRI	GGCC	4 cut(s)	276, 324, 457, 1614
BtgI	CCRYGG	1 cut(s)	464
BtgZI	GCGATG	1 cut(s)	980
BtrI	CACGTC	1 cut(s)	1098
BtsCI	GGATG	8 cut(s)	220, 399, 491, 555, 697, 772, 1216, 1372
BtsI	GCAGTG	2 cut(s)	904, 1350
BtsIMutI	CAGTG	3 cut(s)	904, 1350, 1773
BveI	ACCTGC	2 cut(s)	579, 1739
Cac8I	GCNNGC	5 cut(s)	386, 1320, 1488, 1580, 1746
CaiI	CAGNNNCTG	1 cut(s)	1349
CciI	TCATGA	1 cut(s)	1585
Cfr10I	RCCGGY	1 cut(s)	457
Cfr13I	GGNCC	5 cut(s)	275, 460, 630, 782, 930
CseI	GACGC	1 cut(s)	86
Csp6I	GTAC	5 cut(s)	47, 297, 1273, 1427, 1752
CspCI	CAANNNNNGTGG	4 cut(s)	289, 324, 718, 753
CviAII	CATG	8 cut(s)	179, 359, 465, 619, 707, 832, 838, 1586
CviQI	GTAC	5 cut(s)	47, 297, 1273, 1427, 1752
DdeI	CTNAG	3 cut(s)	926, 1182, 1206
DpnI	GATC	6 cut(s)	415, 566, 655, 1071, 1254, 1383
DpnII	GATC	6 cut(s)	413, 564, 653, 1069, 1252, 1381
EaeI	YGGCCR	1 cut(s)	455
EagI	CGGCCG	1 cut(s)	455
Eam1104I	CTCTTC	2 cut(s)	561, 1136
EarI	CTCTTC	2 cut(s)	561, 1136
EclXI	CGGCCG	1 cut(s)	455
Eco105I	TACGTA	1 cut(s)	1272
Eco130I	CCWWGG	1 cut(s)	464
Eco32I	GATATC	1 cut(s)	1725
Eco47I	GGWCC	4 cut(s)	460, 630, 782, 930
Eco52I	CGGCCG	1 cut(s)	455
Eco57I	CTGAAG	3 cut(s)	111, 542, 762
Eco81I	CCTNAGG	1 cut(s)	1182
Eco88I	CYCGRG	1 cut(s)	278
EcoO109I	RGGNCCY	1 cut(s)	930
EcoRI	GAATTC	1 cut(s)	7
EcoRII	CCWGG	2 cut(s)	409, 713
EcoRV	GATATC	1 cut(s)	1725
EcoT14I	CCWWGG	1 cut(s)	464
ErhI	CCWWGG	1 cut(s)	464
FaeI	CATG	8 cut(s)	182, 362, 468, 622, 710, 835, 841, 1589
FaqI	GGGAC	1 cut(s)	446
FatI	CATG	8 cut(s)	178, 358, 464, 618, 706, 831, 837, 1585
FblI	GTMKAC	2 cut(s)	183, 943
Fnu4HI	GCNGC	3 cut(s)	95, 141, 144
FokI	GGATG	8 cut(s)	227, 386, 478, 562, 704, 779, 1223, 1379
Fsp4HI	GCNGC	3 cut(s)	95, 141, 144
FspBI	CTAG	5 cut(s)	702, 801, 1079, 1133, 1319
GluI	GCNGC	3 cut(s)	95, 141, 144
GsuI	CTGGAG	2 cut(s)	697, 1627
HaeIII	GGCC	4 cut(s)	276, 324, 457, 1614
HapII	CCGG	2 cut(s)	458, 689
HgaI	GACGC	1 cut(s)	86
Hin1I	GRCGYC	1 cut(s)	878
Hin1II	CATG	8 cut(s)	182, 362, 468, 622, 710, 835, 841, 1589
HincII	GTYRAC	2 cut(s)	184, 208
HindII	GTYRAC	2 cut(s)	184, 208
HindIII	AAGCTT	2 cut(s)	1374, 1672
HinfI	GANTC	5 cut(s)	34, 107, 596, 710, 846
HpaII	CCGG	2 cut(s)	458, 689
HphI	GGTGA	1 cut(s)	1552
Hpy166II	GTNNAC	5 cut(s)	184, 208, 227, 630, 944
Hpy188I	TCNGA	5 cut(s)	33, 883, 1102, 1359, 1441
Hpy188III	TCNNGA	8 cut(s)	104, 134, 199, 917, 1208, 1260, 1586, 1606
Hpy8I	GTNNAC	5 cut(s)	184, 208, 227, 630, 944
Hpy99I	CGWCG	1 cut(s)	102
HpyAV	CCTTC	3 cut(s)	836, 1357, 1765
HpyCH4III	ACNGT	1 cut(s)	188
HpyCH4IV	ACGT	4 cut(s)	878, 946, 1097, 1271
HpyF10VI	GCNNNNNNNGC	3 cut(s)	149, 1315, 1349
HpyF3I	CTNAG	3 cut(s)	926, 1182, 1206
HpySE526I	ACGT	4 cut(s)	878, 946, 1097, 1271
Hsp92I	GRCGYC	1 cut(s)	878
Hsp92II	CATG	8 cut(s)	182, 362, 468, 622, 710, 835, 841, 1589
KpnI	GGTACC	1 cut(s)	300
Kzo9I	GATC	6 cut(s)	413, 564, 653, 1069, 1252, 1381
LguI	GCTCTTC	1 cut(s)	561
LmnI	GCTCC	1 cut(s)	122
Lsp1109I	GCAGC	3 cut(s)	81, 127, 155
LweI	GCATC	5 cut(s)	757, 781, 1104, 1231, 1774
MaeI	CTAG	5 cut(s)	702, 801, 1079, 1133, 1319
MaeII	ACGT	4 cut(s)	878, 946, 1097, 1271
MaeIII	GTNAC	4 cut(s)	487, 1345, 1540, 1625
MalI	GATC	6 cut(s)	415, 566, 655, 1071, 1254, 1383
MboI	GATC	6 cut(s)	413, 564, 653, 1069, 1252, 1381
MboII	GAAGA	4 cut(s)	548, 768, 983, 1153
MfeI	CAATTG	2 cut(s)	660, 1738
MflI	RGATCY	1 cut(s)	1069
MhlI	GDGCHC	2 cut(s)	358, 855
MlyI	GAGTC	2 cut(s)	101, 704
MmeI	TCCRAC	1 cut(s)	631
MroXI	GAANNNNTTC	3 cut(s)	614, 906, 990
MseI	TTAA	4 cut(s)	291, 1218, 1295, 1676
MslI	CAYNNNNRTG	3 cut(s)	836, 1192, 1590
MspCI	CTTAAG	1 cut(s)	1675
MspI	CCGG	2 cut(s)	458, 689
MspR9I	CCNGG	3 cut(s)	411, 689, 715
MunI	CAATTG	2 cut(s)	660, 1738
Mva1269I	GAATGC	3 cut(s)	581, 1345, 1531
MvaI	CCWGG	2 cut(s)	411, 715
MwoI	GCNNNNNNNGC	3 cut(s)	149, 1315, 1349
NciI	CCSGG	1 cut(s)	689
NcoI	CCATGG	1 cut(s)	464
NdeII	GATC	6 cut(s)	413, 564, 653, 1069, 1252, 1381
NheI	GCTAGC	1 cut(s)	1318
NlaIII	CATG	8 cut(s)	182, 362, 468, 622, 710, 835, 841, 1589
NlaIV	GGNNCC	6 cut(s)	277, 298, 462, 743, 909, 1071
NmuCI	GTSAC	2 cut(s)	1345, 1540
NspI	RCATGY	1 cut(s)	835
OliI	CACNNNNGTG	1 cut(s)	1192
PagI	TCATGA	1 cut(s)	1585
PciSI	GCTCTTC	1 cut(s)	561
PcsI	WCGNNNNNNNCGW	1 cut(s)	189
PctI	GAATGC	3 cut(s)	581, 1345, 1531
PdmI	GAANNNNTTC	3 cut(s)	614, 906, 990
PfeI	GAWTC	3 cut(s)	34, 596, 846
PfoI	TCCNGGA	2 cut(s)	409, 687
PkrI	GCNGC	3 cut(s)	96, 142, 145
PleI	GAGTC	2 cut(s)	101, 704
PpsI	GAGTC	2 cut(s)	101, 704
Ppu21I	YACGTR	1 cut(s)	1272
PpuMI	RGGWCCY	1 cut(s)	930
PshBI	ATTAAT	1 cut(s)	1218
PsiI	TTATAA	1 cut(s)	1008
Psp5II	RGGWCCY	1 cut(s)	930
Psp6I	CCWGG	2 cut(s)	409, 713
PspGI	CCWGG	2 cut(s)	409, 713
PspN4I	GGNNCC	6 cut(s)	277, 298, 462, 743, 909, 1071
PspPI	GGNCC	5 cut(s)	275, 460, 630, 782, 930
PspPPI	RGGWCCY	1 cut(s)	930
PstI	CTGCAG	1 cut(s)	145
PstNI	CAGNNNCTG	1 cut(s)	1349
PsuI	RGATCY	1 cut(s)	1069
RsaI	GTAC	5 cut(s)	48, 298, 1274, 1428, 1753
RsaNI	GTAC	5 cut(s)	47, 297, 1273, 1427, 1752
RseI	CAYNNNNRTG	3 cut(s)	836, 1192, 1590
SalI	GTCGAC	1 cut(s)	182
SapI	GCTCTTC	1 cut(s)	561
SaqAI	TTAA	4 cut(s)	291, 1218, 1295, 1676
SatI	GCNGC	3 cut(s)	95, 141, 144
Sau3AI	GATC	6 cut(s)	413, 564, 653, 1069, 1252, 1381
Sau96I	GGNCC	5 cut(s)	275, 460, 630, 782, 930
SchI	GAGTC	2 cut(s)	101, 704
ScrFI	CCNGG	3 cut(s)	411, 689, 715
SduI	GDGCHC	2 cut(s)	358, 855
SfaNI	GCATC	5 cut(s)	757, 781, 1104, 1231, 1774
SfcI	CTRYAG	1 cut(s)	141
SinI	GGWCC	4 cut(s)	460, 630, 782, 930
SmiMI	CAYNNNNRTG	3 cut(s)	836, 1192, 1590
SmlI	CTYRAG	1 cut(s)	1675
SmoI	CTYRAG	1 cut(s)	1675
SnaBI	TACGTA	1 cut(s)	1272
SpeI	ACTAGT	1 cut(s)	800
SspI	AATATT	1 cut(s)	1820
SspMI	CTAG	5 cut(s)	702, 801, 1079, 1133, 1319
StyD4I	CCNGG	3 cut(s)	409, 687, 713
StyI	CCWWGG	1 cut(s)	464
TaaI	ACNGT	1 cut(s)	188
TaiI	ACGT	4 cut(s)	881, 949, 1100, 1274
TaqI	TCGA	6 cut(s)	5, 183, 192, 268, 849, 1091
TatI	WGTACW	1 cut(s)	1426
TfiI	GAWTC	3 cut(s)	34, 596, 846
Tru1I	TTAA	4 cut(s)	291, 1218, 1295, 1676
Tru9I	TTAA	4 cut(s)	291, 1218, 1295, 1676
TscAI	CASTG	3 cut(s)	904, 1350, 1773
TseFI	GTSAC	2 cut(s)	1345, 1540
TseI	GCWGC	3 cut(s)	94, 140, 143
Tsp45I	GTSAC	2 cut(s)	1345, 1540
TspGWI	ACGGA	1 cut(s)	1517
TspRI	CASTG	3 cut(s)	904, 1350, 1773
Vha464I	CTTAAG	1 cut(s)	1675
VpaK11BI	GGWCC	4 cut(s)	460, 630, 782, 930
VspI	ATTAAT	1 cut(s)	1218
XapI	RAATTY	4 cut(s)	7, 447, 808, 987
XceI	RCATGY	1 cut(s)	835
XcmI	CCANNNNNNNNNTGG	1 cut(s)	918
XmiI	GTMKAC	2 cut(s)	183, 943
XmnI	GAANNNNTTC	3 cut(s)	614, 906, 990
XspI	CTAG	5 cut(s)	702, 801, 1079, 1133, 1319
ZraI	GACGTC	1 cut(s)	879

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.