Rh7DG028000

alpha-N-acetylglucosaminidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000933
Physical Location & Seq
Forward (+)
2102807 .. 2109975
7169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG028000.1

Sequence Viewer

Length: 1500 bp
ATGGCTCCCACCCCATTTCCCGCCATTTCTACCATTACCATCTTCACGCTCTTCTTCTTCTTCTTCTTCTTCACCTTGTGTGTCCTCTCCTCATCATCATCCACCATCGGAGTCGGCTACATATCGAGCGTTCTGGAACTTCAGGACCGCGAGAGGGCCTCTCCCTATGTCCAAGTCGCCGCCGCTCGCGGAGTACTCCACCGCCTTCTTCCTTCCCAATCCTCCAGCTTCGACTTCCAAATCGTCTCTAAGGAGCAATGTGGTGGTGCTTCCTGCTTTATGATTAAGAACCATCCTTCTTTTCGTAGACGAGGGGATCCACAGATTCTGATTTCCGGAGTCACTGGAGTGGAGATTTTGGCTGGTCTGCATTGGTATTTGAAGAATTGGTGTGGCTCACATATATCTTGGGATAAAACTGGTGGTGCGCAACTATTTTCAGTCCCAAAAGCAGGGTTTCTATCTCGTGTAGGAGATGGTGGCATTTTAGTCCAGAGACCTGTTCCTTGGAACTATTACCAGAATGCTGTCACATCTAGCTATTCTTTGGCTTGGTGGGACTGGAATAGATGGGAAAAGGAGATTGATATGATGGCCCTCCAGGGTATCAATTTGCCACTAGCATTTACAGGTCAAGAGGCTATATGGCAGAAAGTTTTCGAGAAATTTAATATGAGCAAATCAGATTTGGATGATTTCTTTGGAGGCCCTGCATTCCTTGCATGGTCTCGAATGGGAAATTTGCATGGATGGGGTGGACCTCTCCCACAAAGTTGGCTTGATCAACAGCTGATTCTGCAGAAAAAAATTCTCAACAGAATGTGTATGCTGCACAACTTTGCAGGAAACGTTGAGATGTATGGCGTTATGGATGCAATAGCATCTGGACCAATTGATGCTTGGACAAGTGAAAACTCAACCATGGTTGGGGTTGGAATGTCAATGGAAGGTATAGAACAGAACCCTGTTGTTTATGATCTCATGTCTGAGATGGCTTTTCAACAGAACAAAGTTGATGTTAAGGACTGGATTAATCTGTATTCAACGAGACGTTATGGTCGTGCAGTTCCTTTAGTGCAACGTGCCTGGAGTATATTACATCATACCATCTATAATTGCACTGATGGTGCCTACGACAAAAATAGGGATGTGATTGTGGCATTTCCGGACGTTGATCCATCCTTTATAGCAAGACCGTCTCAAGGTTATTACCAAAATGATAAATCATTAGTATCAAGAAGATCAGTCCTGAAGGAAATAACTGATTCATTTGAGCGACCTCATTTATGGTACTCGACTTCTGAAGTAGTACATGCATTAGAACTTTTTATTGCAAGTGGAGATGAACTCTCAGAAAGCAACACTTACAGGTACGTAATCAGCCCTTTCAACTTTGGAAACCTGACAACACTGCTCACGACATACACAACTCCCTACAGAACTTCTGTAACTAAATTTGTCACATGTAAAATGTACTCAGGTATGACCTTGTTGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
NAGLU NAGLU_C NAGLU_N

Protein Analysis

499

Amino Acids

56.38

Weight (kDa)

6.56

Isoelectric Point (pI)

46.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAGLU_N PF12971 60 - 145 3e-21 Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain
NAGLU PF05089 172 - 274 8.6e-49 Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
NAGLU PF05089 275 - 334 4e-18 Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
NAGLU_C PF12972 343 - 458 4.2e-20 Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1056
Acc16I TGCGCA 1 cut(s) 429
AccB1I GGYRCC 1 cut(s) 1127
AccBSI CCGCTC 1 cut(s) 185
AccI GTMKAC 1 cut(s) 307
AccII CGCG 2 cut(s) 150, 189
AccIII TCCGGA 2 cut(s) 335, 1165
AciI CCGC 6 cut(s) 21, 148, 180, 183, 189, 202
AclI AACGTT 1 cut(s) 849
AclWI GGATC 3 cut(s) 311, 324, 1169
AcsI RAATTY 4 cut(s) 665, 739, 807, 1454
AcuI CTGAAG 3 cut(s) 125, 1271, 1323
AdeI CACNNNGTG 1 cut(s) 78
AfaI GTAC 5 cut(s) 195, 1292, 1311, 1373, 1475
AfiI CCNNNNNNNGG 4 cut(s) 154, 452, 927, 1202
AflIII ACRYGT 1 cut(s) 1463
AgsI TTSAA 4 cut(s) 382, 1001, 1044, 1390
AjnI CCWGG 2 cut(s) 600, 1085
AleI CACNNNNGTG 1 cut(s) 347
AluBI AGCT 3 cut(s) 228, 540, 790
AluI AGCT 3 cut(s) 228, 540, 790
Alw26I GTCTC 5 cut(s) 250, 490, 732, 1042, 1203
AlwI GGATC 3 cut(s) 311, 324, 1169
AlwNI CAGNNNCTG 1 cut(s) 328
Aor13HI TCCGGA 2 cut(s) 335, 1165
AoxI GGCC 3 cut(s) 156, 594, 706
ApeKI GCWGC 1 cut(s) 829
ApoI RAATTY 4 cut(s) 665, 739, 807, 1454
AseI ATTAAT 1 cut(s) 1032
Asp700I GAANNNNTTC 1 cut(s) 656
AspLEI GCGC 1 cut(s) 430
AspS9I GGNCC 6 cut(s) 145, 156, 595, 707, 758, 887
AsuHPI GGTGA 1 cut(s) 64
AvaII GGWCC 3 cut(s) 145, 758, 887
BamHI GGATCC 1 cut(s) 316
BanI GGYRCC 1 cut(s) 1127
BauI CACGAG 1 cut(s) 465
BbvI GCAGC 1 cut(s) 816
BciT130I CCWGG 2 cut(s) 602, 1087
BclI TGATCA 1 cut(s) 781
BcoDI GTCTC 5 cut(s) 250, 490, 732, 1042, 1203
BfaI CTAG 2 cut(s) 537, 620
BfmI CTRYAG 2 cut(s) 797, 1435
BisI GCNGC 3 cut(s) 180, 183, 830
BlsI GCNGC 3 cut(s) 181, 184, 831
BmcAI AGTACT 1 cut(s) 195
Bme1390I CCNGG 2 cut(s) 602, 1087
Bme18I GGWCC 3 cut(s) 145, 758, 887
BmgT120I GGNCC 6 cut(s) 145, 156, 595, 707, 758, 887
BmiI GGNNCC 3 cut(s) 6, 318, 1129
BmrFI CCNGG 2 cut(s) 602, 1087
BmsI GCATC 3 cut(s) 862, 886, 890
BplI GAGNNNNNCTC 6 cut(s) 143, 175, 145, 177, 1332, 1364
BpmI CTGGAG 4 cut(s) 208, 366, 584, 1108
BpuEI CTTGAG 1 cut(s) 1185
BsaAI YACGTR 1 cut(s) 1375
BsaI GGTCTC 2 cut(s) 490, 732
BsaJI CCNNGG 3 cut(s) 506, 601, 921
BsaWI WCCGGW 2 cut(s) 335, 1165
Bsc4I CCNNNNNNNGG 4 cut(s) 154, 452, 927, 1202
Bse1I ACTGG 4 cut(s) 349, 424, 566, 1031
Bse3DI GCAATG 1 cut(s) 263
BseAI TCCGGA 2 cut(s) 335, 1165
BseBI CCWGG 2 cut(s) 602, 1087
BseDI CCNNGG 3 cut(s) 506, 601, 921
BseGI GGATG 7 cut(s) 98, 292, 697, 755, 877, 1153, 1178
BseLI CCNNNNNNNGG 4 cut(s) 154, 452, 927, 1202
BseMI GCAATG 1 cut(s) 263
BseMII CTCAG 3 cut(s) 978, 1365, 1491
BseNI ACTGG 4 cut(s) 349, 424, 566, 1031
BseRI GAGGAG 1 cut(s) 79
BseXI GCAGC 1 cut(s) 816
BsgI GTGCAG 2 cut(s) 815, 1083
Bsh1236I CGCG 2 cut(s) 150, 189
BshFI GGCC 3 cut(s) 158, 596, 708
BshNI GGYRCC 1 cut(s) 1127
BsiSI CCGG 2 cut(s) 336, 1166
BslFI GGGAC 2 cut(s) 428, 572
BslI CCNNNNNNNGG 4 cut(s) 154, 452, 927, 1202
BsmAI GTCTC 5 cut(s) 250, 490, 732, 1042, 1203
BsmBI CGTCTC 3 cut(s) 250, 1042, 1203
BsmFI GGGAC 2 cut(s) 428, 572
BsmI GAATGC 2 cut(s) 529, 713
BsnI GGCC 3 cut(s) 158, 596, 708
Bso31I GGTCTC 2 cut(s) 490, 732
Bsp13I TCCGGA 2 cut(s) 335, 1165
Bsp143I GATC 5 cut(s) 316, 781, 976, 1174, 1241
Bsp19I CCATGG 1 cut(s) 921
BspACI CCGC 6 cut(s) 21, 148, 180, 183, 189, 202
BspANI GGCC 3 cut(s) 158, 596, 708
BspCNI CTCAG 3 cut(s) 979, 1364, 1490
BspEI TCCGGA 2 cut(s) 335, 1165
BspFNI CGCG 2 cut(s) 150, 189
BspLI GGNNCC 3 cut(s) 6, 318, 1129
BspMAI CTGCAG 1 cut(s) 801
BspPI GGATC 3 cut(s) 311, 324, 1169
BspQI GCTCTTC 1 cut(s) 56
BspT107I GGYRCC 1 cut(s) 1127
BspTNI GGTCTC 2 cut(s) 490, 732
BsrBI CCGCTC 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 263
BsrI ACTGG 4 cut(s) 349, 424, 566, 1031
BssECI CCNNGG 3 cut(s) 506, 601, 921
BssMI GATC 5 cut(s) 316, 781, 976, 1174, 1241
BssSI CACGAG 1 cut(s) 465
BssT1I CCWWGG 2 cut(s) 506, 921
Bst2BI CACGAG 1 cut(s) 465
Bst2UI CCWGG 2 cut(s) 602, 1087
Bst4CI ACNGT 1 cut(s) 1197
Bst6I CTCTTC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 719
BstBAI YACGTR 1 cut(s) 1375
BstC8I GCNNGC 1 cut(s) 187
BstDEI CTNAG 4 cut(s) 249, 987, 1351, 1477
BstDSI CCRYGG 1 cut(s) 921
BstF5I GGATG 7 cut(s) 98, 292, 697, 755, 877, 1153, 1178
BstFNI CGCG 2 cut(s) 150, 189
BstHHI GCGC 1 cut(s) 430
BstKTI GATC 5 cut(s) 319, 784, 979, 1177, 1244
BstMAI GTCTC 5 cut(s) 250, 490, 732, 1042, 1203
BstMBI GATC 5 cut(s) 316, 781, 976, 1174, 1241
BstMWI GCNNNNNNNGC 2 cut(s) 719, 796
BstNI CCWGG 2 cut(s) 602, 1087
BstNSI RCATGY 2 cut(s) 1316, 1467
BstSCI CCNGG 2 cut(s) 600, 1085
BstSFI CTRYAG 2 cut(s) 797, 1435
BstSNI TACGTA 1 cut(s) 1375
BstUI CGCG 2 cut(s) 150, 189
BstV1I GCAGC 1 cut(s) 816
BstX2I RGATCY 1 cut(s) 316
BstXI CCANNNNNNTGG 1 cut(s) 774
BstYI RGATCY 1 cut(s) 316
BsuRI GGCC 3 cut(s) 158, 596, 708
BtgI CCRYGG 1 cut(s) 921
BtsCI GGATG 7 cut(s) 98, 292, 697, 755, 877, 1153, 1178
BtsI GCAGTG 1 cut(s) 1409
BtsIMutI CAGTG 3 cut(s) 342, 1119, 1409
Cac8I GCNNGC 1 cut(s) 187
CaiI CAGNNNCTG 1 cut(s) 328
CfoI GCGC 1 cut(s) 430
Cfr13I GGNCC 6 cut(s) 145, 156, 595, 707, 758, 887
Csp6I GTAC 5 cut(s) 194, 1291, 1310, 1372, 1474
CviAII CATG 6 cut(s) 723, 746, 922, 982, 1313, 1464
CviQI GTAC 5 cut(s) 194, 1291, 1310, 1372, 1474
DdeI CTNAG 4 cut(s) 249, 987, 1351, 1477
DpnI GATC 5 cut(s) 318, 783, 978, 1176, 1243
DpnII GATC 5 cut(s) 316, 781, 976, 1174, 1241
DraIII CACNNNGTG 1 cut(s) 78
DrdI GACNNNNNNGTC 1 cut(s) 1056
DseDI GACNNNNNNGTC 1 cut(s) 1056
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco105I TACGTA 1 cut(s) 1375
Eco130I CCWWGG 2 cut(s) 506, 921
Eco31I GGTCTC 2 cut(s) 490, 732
Eco47I GGWCC 3 cut(s) 145, 758, 887
Eco57I CTGAAG 3 cut(s) 125, 1271, 1323
EcoO109I RGGNCCY 2 cut(s) 156, 707
EcoRII CCWGG 2 cut(s) 600, 1085
EcoT14I CCWWGG 2 cut(s) 506, 921
EcoT22I ATGCAT 1 cut(s) 1318
ErhI CCWWGG 2 cut(s) 506, 921
Esp3I CGTCTC 3 cut(s) 250, 1042, 1203
FaeI CATG 6 cut(s) 726, 749, 925, 985, 1316, 1467
FalI AAGNNNNNCTT 2 cut(s) 1348, 1380
FaqI GGGAC 2 cut(s) 428, 572
FatI CATG 6 cut(s) 722, 745, 921, 981, 1312, 1463
FauI CCCGC 1 cut(s) 28
FbaI TGATCA 1 cut(s) 781
FblI GTMKAC 1 cut(s) 307
Fnu4HI GCNGC 3 cut(s) 180, 183, 830
FokI GGATG 7 cut(s) 85, 279, 704, 762, 884, 1160, 1165
Fsp4HI GCNGC 3 cut(s) 180, 183, 830
FspBI CTAG 2 cut(s) 537, 620
FspI TGCGCA 1 cut(s) 429
GlaI GCGC 1 cut(s) 429
GluI GCNGC 3 cut(s) 180, 183, 830
GsuI CTGGAG 4 cut(s) 208, 366, 584, 1108
HaeIII GGCC 3 cut(s) 158, 596, 708
HapII CCGG 2 cut(s) 336, 1166
HhaI GCGC 1 cut(s) 430
Hin1II CATG 6 cut(s) 726, 749, 925, 985, 1316, 1467
Hin6I GCGC 1 cut(s) 428
HinP1I GCGC 1 cut(s) 428
HincII GTYRAC 1 cut(s) 1494
HindII GTYRAC 1 cut(s) 1494
HinfI GANTC 5 cut(s) 111, 325, 339, 793, 1265
HpaII CCGG 2 cut(s) 336, 1166
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 3 cut(s) 308, 758, 1494
Hpy188I TCNGA 6 cut(s) 110, 330, 685, 988, 1303, 1354
Hpy8I GTNNAC 3 cut(s) 308, 758, 1494
HpyAV CCTTC 5 cut(s) 215, 222, 306, 941, 1246
HpyCH4III ACNGT 1 cut(s) 1197
HpyCH4IV ACGT 5 cut(s) 849, 1051, 1081, 1170, 1374
HpyF10VI GCNNNNNNNGC 2 cut(s) 719, 796
HpyF3I CTNAG 4 cut(s) 249, 987, 1351, 1477
HpySE526I ACGT 5 cut(s) 849, 1051, 1081, 1170, 1374
Hsp92II CATG 6 cut(s) 726, 749, 925, 985, 1316, 1467
HspAI GCGC 1 cut(s) 428
Kpn2I TCCGGA 2 cut(s) 335, 1165
Ksp22I TGATCA 1 cut(s) 781
Kzo9I GATC 5 cut(s) 316, 781, 976, 1174, 1241
LguI GCTCTTC 1 cut(s) 56
LmnI GCTCC 2 cut(s) 10, 253
Lsp1109I GCAGC 1 cut(s) 816
LweI GCATC 3 cut(s) 862, 886, 890
MaeI CTAG 2 cut(s) 537, 620
MaeII ACGT 5 cut(s) 849, 1051, 1081, 1170, 1374
MaeIII GTNAC 4 cut(s) 340, 529, 1447, 1459
MalI GATC 5 cut(s) 318, 783, 978, 1176, 1243
MbiI CCGCTC 1 cut(s) 185
MboI GATC 5 cut(s) 316, 781, 976, 1174, 1241
MfeI CAATTG 1 cut(s) 891
MflI RGATCY 1 cut(s) 316
MluCI AATT 8 cut(s) 385, 610, 665, 739, 807, 891, 1114, 1454
MlyI GAGTC 2 cut(s) 120, 348
MmeI TCCRAC 1 cut(s) 913
Mph1103I ATGCAT 1 cut(s) 1318
MroI TCCGGA 2 cut(s) 335, 1165
MroXI GAANNNNTTC 1 cut(s) 656
MseI TTAA 4 cut(s) 285, 669, 1020, 1032
MslI CAYNNNNRTG 1 cut(s) 347
MspA1I CMGCKG 1 cut(s) 790
MspI CCGG 2 cut(s) 336, 1166
MspR9I CCNGG 2 cut(s) 602, 1087
MunI CAATTG 1 cut(s) 891
Mva1269I GAATGC 2 cut(s) 529, 713
MvaI CCWGG 2 cut(s) 602, 1087
MvnI CGCG 2 cut(s) 150, 189
MwoI GCNNNNNNNGC 2 cut(s) 719, 796
NcoI CCATGG 1 cut(s) 921
NdeII GATC 5 cut(s) 316, 781, 976, 1174, 1241
NlaIII CATG 6 cut(s) 726, 749, 925, 985, 1316, 1467
NlaIV GGNNCC 3 cut(s) 6, 318, 1129
NmuCI GTSAC 3 cut(s) 340, 529, 1459
NsbI TGCGCA 1 cut(s) 429
NsiI ATGCAT 1 cut(s) 1318
NspI RCATGY 2 cut(s) 1316, 1467
OliI CACNNNNGTG 1 cut(s) 347
PciI ACATGT 1 cut(s) 1463
PciSI GCTCTTC 1 cut(s) 56
PcsI WCGNNNNNNNCGW 1 cut(s) 1057
PctI GAATGC 2 cut(s) 529, 713
PdmI GAANNNNTTC 1 cut(s) 656
PfeI GAWTC 3 cut(s) 325, 793, 1265
PkrI GCNGC 3 cut(s) 181, 184, 831
PleI GAGTC 2 cut(s) 119, 347
PpsI GAGTC 2 cut(s) 119, 347
Ppu21I YACGTR 1 cut(s) 1375
PscI ACATGT 1 cut(s) 1463
PshBI ATTAAT 1 cut(s) 1032
Psp1406I AACGTT 1 cut(s) 849
Psp6I CCWGG 2 cut(s) 600, 1085
PspGI CCWGG 2 cut(s) 600, 1085
PspN4I GGNNCC 3 cut(s) 6, 318, 1129
PspPI GGNCC 6 cut(s) 145, 156, 595, 707, 758, 887
PstI CTGCAG 1 cut(s) 801
PstNI CAGNNNCTG 1 cut(s) 328
PsuI RGATCY 1 cut(s) 316
PvuII CAGCTG 1 cut(s) 790
RsaI GTAC 5 cut(s) 195, 1292, 1311, 1373, 1475
RsaNI GTAC 5 cut(s) 194, 1291, 1310, 1372, 1474
RseI CAYNNNNRTG 1 cut(s) 347
SapI GCTCTTC 1 cut(s) 56
SaqAI TTAA 4 cut(s) 285, 669, 1020, 1032
SatI GCNGC 3 cut(s) 180, 183, 830
Sau3AI GATC 5 cut(s) 316, 781, 976, 1174, 1241
Sau96I GGNCC 6 cut(s) 145, 156, 595, 707, 758, 887
ScaI AGTACT 1 cut(s) 195
SchI GAGTC 2 cut(s) 120, 348
ScrFI CCNGG 2 cut(s) 602, 1087
SfaNI GCATC 3 cut(s) 862, 886, 890
SfcI CTRYAG 2 cut(s) 797, 1435
SinI GGWCC 3 cut(s) 145, 758, 887
SmiMI CAYNNNNRTG 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 1200
SmoI CTYRAG 1 cut(s) 1200
SnaBI TACGTA 1 cut(s) 1375
Sse9I AATT 8 cut(s) 385, 610, 665, 739, 807, 891, 1114, 1454
SsiI CCGC 6 cut(s) 21, 148, 180, 183, 189, 202
SspMI CTAG 2 cut(s) 537, 620
StyD4I CCNGG 2 cut(s) 600, 1085
StyI CCWWGG 2 cut(s) 506, 921
TaaI ACNGT 1 cut(s) 1197
TaiI ACGT 5 cut(s) 852, 1054, 1084, 1173, 1377
TaqI TCGA 5 cut(s) 125, 231, 660, 730, 1295
TasI AATT 8 cut(s) 385, 610, 665, 739, 807, 891, 1114, 1454
TatI WGTACW 3 cut(s) 193, 1309, 1473
TauI GCSGC 2 cut(s) 182, 185
TfiI GAWTC 3 cut(s) 325, 793, 1265
Tru1I TTAA 4 cut(s) 285, 669, 1020, 1032
Tru9I TTAA 4 cut(s) 285, 669, 1020, 1032
TscAI CASTG 3 cut(s) 349, 1126, 1416
TseFI GTSAC 3 cut(s) 340, 529, 1459
TseI GCWGC 1 cut(s) 829
Tsp45I GTSAC 3 cut(s) 340, 529, 1459
TspDTI ATGAA 2 cut(s) 1257, 1359
TspRI CASTG 3 cut(s) 349, 1126, 1416
VpaK11BI GGWCC 3 cut(s) 145, 758, 887
VspI ATTAAT 1 cut(s) 1032
XapI RAATTY 4 cut(s) 665, 739, 807, 1454
XceI RCATGY 2 cut(s) 1316, 1467
XcmI CCANNNNNNNNNTGG 1 cut(s) 897
XmiI GTMKAC 1 cut(s) 307
XmnI GAANNNNTTC 1 cut(s) 656
XspI CTAG 2 cut(s) 537, 620
ZrmI AGTACT 1 cut(s) 195
Zsp2I ATGCAT 1 cut(s) 1318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.