RchiOBHm_Chr7g0179741

alpha-N-acetylglucosaminidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000933
Physical Location & Seq
Forward (+)
1930109 .. 1932691
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16024

Sequence Viewer

Length: 1098 bp
ATGGATGCAATAGCATCTGGACCAATTGATGCTTGGACAAGTGAAAACTCAACCATGGTTGGGGTTGGAATGTCAATGGAAGGTATAGAACAGAACCCTGTTGTTTATGATCTCATGTCTGAGATGGCTTTTCAACAGAACAAAGTTGATGTTAAGGACTGGATTAATCTGTATTCAACGAGACGTTATGGTCGTGCAGTTCCTTTAGTGCAACGTGCCTGGAGTATATTACATCATACCATCTATAATTGCACTGATGGTGCCTACGACAAAAATAGGGATGTGATTGTGTCATTTCCGGACGTTGATCCATCCTTTATAGCAAGACCGTCTCAAGGTTATTACCAAAATGATAAATCATTAGTATCAAGAAGATCAGTCCTGAAGGAAATAACTGATTCATTTGAGCGACCTCATTTATGGTACTCGACTTCTGAAGTAGTACATGCATTAGAACTTTTTATTGCAAGTGGAGATGAACTCTCAGAAAGCAACACTTACAGGTATGACCTTGTTGACCTGACCAGACAAGCTTTAGCTAAGTATGCAAACCAGCTGTTTCTGAAGGTCGTAGAAGCCTATCACTTAAATGATACCCTTGAAGTAGTTGGCCTCAGCCAGAAGTTTTTGGAACTGGTTGAAGATATGGACACACTTTTAGCATGCCATGATGGATTTCTTCTTGGACCATGGTTGGAAAGCGCAAAGAAACTCGCCCAAGACAAAGAGCAGCAAATACAGTTTGAATGGAATGCTAGAACTCAAATAACTATGTGGTTTGACAACACAGAAGAGGAAGCAAGTCTTCTTCGCGACTATGGAAACAAGTATTGGAGTGGGCTTCTGCGAGACTACTATGGTCCCCGAGCAGCCATATACTTCAAGTATCTGATAAAAAGCTTGGACGAGGGTTATGATTTTGACTTGAAGAACTGGAGGAGGGAGTGGATCAAGCTCACCAATGACTGGCAAAACAGCAGGAACACATTCCCGGTGAAAAGCACCGGAAATGCGGTGACTACTTCTCGGTTGCTTTTCGAAAAATACTTACGAAGCGCTGACATAGAATCACTGGATGAAGCTGCTATTATGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
NAGLU NAGLU_C NAGLU_N

Protein Analysis

365

Amino Acids

42.31

Weight (kDa)

4.89

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAGLU PF05089 2 - 45 5.8e-10 Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain
NAGLU_C PF12972 54 - 348 2.4e-87 Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 189
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 966
AccII CGCG 1 cut(s) 813
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 1 cut(s) 1013
AclWI GGATC 2 cut(s) 302, 956
AcuI CTGAAG 3 cut(s) 404, 456, 584
AfaI GTAC 2 cut(s) 425, 444
AfeI AGCGCT 1 cut(s) 1057
AfiI CCNNNNNNNGG 3 cut(s) 60, 335, 966
AgsI TTSAA 7 cut(s) 134, 177, 602, 641, 746, 883, 928
AjnI CCWGG 1 cut(s) 218
AjuI GAANNNNNNNTTGG 2 cut(s) 814, 846
AluBI AGCT 6 cut(s) 533, 539, 556, 900, 955, 1082
AluI AGCT 6 cut(s) 533, 539, 556, 900, 955, 1082
Alw26I GTCTC 3 cut(s) 175, 336, 843
AlwI GGATC 2 cut(s) 302, 956
Ama87I CYCGRG 1 cut(s) 864
Aor13HI TCCGGA 1 cut(s) 298
Aor51HI AGCGCT 1 cut(s) 1057
AoxI GGCC 1 cut(s) 610
ApeKI GCWGC 3 cut(s) 730, 869, 1082
AseI ATTAAT 1 cut(s) 165
Asp700I GAANNNNTTC 1 cut(s) 986
AspLEI GCGC 2 cut(s) 704, 1058
AspS9I GGNCC 3 cut(s) 20, 686, 860
AsuC2I CCSGG 1 cut(s) 992
AsuHPI GGTGA 3 cut(s) 949, 1006, 1027
AsuII TTCGAA 1 cut(s) 1038
AvaI CYCGRG 1 cut(s) 864
AvaII GGWCC 3 cut(s) 20, 686, 860
BanI GGYRCC 1 cut(s) 260
BbsI GAAGAC 1 cut(s) 797
BbvCI CCTCAGC 1 cut(s) 614
BbvI GCAGC 3 cut(s) 742, 881, 1069
BccI CCATC 5 cut(s) 118, 248, 251, 319, 665
BciT130I CCWGG 1 cut(s) 220
BcnI CCSGG 1 cut(s) 992
BcoDI GTCTC 3 cut(s) 175, 336, 843
BfaI CTAG 1 cut(s) 756
BfoI RGCGCY 1 cut(s) 1059
BisI GCNGC 3 cut(s) 731, 870, 1083
BlsI GCNGC 3 cut(s) 732, 871, 1084
Bme1390I CCNGG 2 cut(s) 220, 992
Bme18I GGWCC 3 cut(s) 20, 686, 860
BmeT110I CYCGRG 1 cut(s) 864
BmgT120I GGNCC 3 cut(s) 20, 686, 860
BmiI GGNNCC 2 cut(s) 262, 862
BmrFI CCNGG 2 cut(s) 220, 992
BmsI GCATC 2 cut(s) 19, 23
BpiI GAAGAC 1 cut(s) 797
BplI GAGNNNNNCTC 2 cut(s) 465, 497
BpmI CTGGAG 2 cut(s) 241, 955
Bpu10I CCTNAGC 1 cut(s) 614
Bpu14I TTCGAA 1 cut(s) 1038
BpuEI CTTGAG 1 cut(s) 318
BpuMI CCSGG 1 cut(s) 992
BsaJI CCNNGG 2 cut(s) 54, 689
BsaWI WCCGGW 2 cut(s) 298, 1004
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 335, 966
Bse1I ACTGG 5 cut(s) 164, 639, 938, 971, 1077
BseAI TCCGGA 1 cut(s) 298
BseBI CCWGG 1 cut(s) 220
BseDI CCNNGG 2 cut(s) 54, 689
BseGI GGATG 4 cut(s) 10, 286, 311, 1081
BseLI CCNNNNNNNGG 3 cut(s) 60, 335, 966
BseMII CTCAG 3 cut(s) 111, 498, 628
BseNI ACTGG 5 cut(s) 164, 639, 938, 971, 1077
BseRI GAGGAG 1 cut(s) 952
BseXI GCAGC 3 cut(s) 742, 881, 1069
BsgI GTGCAG 1 cut(s) 216
Bsh1236I CGCG 1 cut(s) 813
BshFI GGCC 1 cut(s) 612
BshNI GGYRCC 1 cut(s) 260
BsiHKCI CYCGRG 1 cut(s) 864
BsiSI CCGG 3 cut(s) 299, 992, 1005
BslFI GGGAC 1 cut(s) 846
BslI CCNNNNNNNGG 3 cut(s) 60, 335, 966
BsmAI GTCTC 3 cut(s) 175, 336, 843
BsmBI CGTCTC 2 cut(s) 175, 336
BsmFI GGGAC 1 cut(s) 846
BsmI GAATGC 1 cut(s) 757
BsnI GGCC 1 cut(s) 612
BsoBI CYCGRG 1 cut(s) 864
Bsp119I TTCGAA 1 cut(s) 1038
Bsp13I TCCGGA 1 cut(s) 298
Bsp143I GATC 4 cut(s) 109, 307, 374, 948
Bsp19I CCATGG 2 cut(s) 54, 689
Bsp68I TCGCGA 1 cut(s) 813
BspACI CCGC 1 cut(s) 1013
BspANI GGCC 1 cut(s) 612
BspCNI CTCAG 3 cut(s) 112, 497, 627
BspEI TCCGGA 1 cut(s) 298
BspFNI CGCG 1 cut(s) 813
BspLI GGNNCC 2 cut(s) 262, 862
BspPI GGATC 2 cut(s) 302, 956
BspT104I TTCGAA 1 cut(s) 1038
BspT107I GGYRCC 1 cut(s) 260
BsrI ACTGG 5 cut(s) 164, 639, 938, 971, 1077
BssECI CCNNGG 2 cut(s) 54, 689
BssMI GATC 4 cut(s) 109, 307, 374, 948
BssT1I CCWWGG 2 cut(s) 54, 689
Bst2UI CCWGG 1 cut(s) 220
Bst4CI ACNGT 2 cut(s) 330, 741
Bst6I CTCTTC 1 cut(s) 786
BstBI TTCGAA 1 cut(s) 1038
BstC8I GCNNGC 1 cut(s) 664
BstDEI CTNAG 4 cut(s) 120, 484, 540, 614
BstDSI CCRYGG 2 cut(s) 54, 689
BstF5I GGATG 4 cut(s) 10, 286, 311, 1081
BstFNI CGCG 1 cut(s) 813
BstH2I RGCGCY 1 cut(s) 1059
BstHHI GCGC 2 cut(s) 704, 1058
BstKTI GATC 4 cut(s) 112, 310, 377, 951
BstMAI GTCTC 3 cut(s) 175, 336, 843
BstMBI GATC 4 cut(s) 109, 307, 374, 948
BstMWI GCNNNNNNNGC 1 cut(s) 545
BstNI CCWGG 1 cut(s) 220
BstNSI RCATGY 2 cut(s) 449, 666
BstSCI CCNGG 2 cut(s) 218, 990
BstUI CGCG 1 cut(s) 813
BstV1I GCAGC 3 cut(s) 742, 881, 1069
BstV2I GAAGAC 1 cut(s) 797
BsuRI GGCC 1 cut(s) 612
BtgI CCRYGG 2 cut(s) 54, 689
BtsCI GGATG 4 cut(s) 10, 286, 311, 1081
BtsIMutI CAGTG 2 cut(s) 252, 1070
BtuMI TCGCGA 1 cut(s) 813
Cac8I GCNNGC 1 cut(s) 664
CfoI GCGC 2 cut(s) 704, 1058
Cfr13I GGNCC 3 cut(s) 20, 686, 860
Csp6I GTAC 2 cut(s) 424, 443
CviAII CATG 6 cut(s) 55, 115, 446, 663, 668, 690
CviQI GTAC 2 cut(s) 424, 443
DdeI CTNAG 4 cut(s) 120, 484, 540, 614
DpnI GATC 4 cut(s) 111, 309, 376, 950
DpnII GATC 4 cut(s) 109, 307, 374, 948
DrdI GACNNNNNNGTC 1 cut(s) 189
DseDI GACNNNNNNGTC 1 cut(s) 189
Eam1104I CTCTTC 1 cut(s) 786
EarI CTCTTC 1 cut(s) 786
Eco130I CCWWGG 2 cut(s) 54, 689
Eco47I GGWCC 3 cut(s) 20, 686, 860
Eco47III AGCGCT 1 cut(s) 1057
Eco57I CTGAAG 3 cut(s) 404, 456, 584
Eco88I CYCGRG 1 cut(s) 864
EcoRII CCWGG 1 cut(s) 218
EcoT14I CCWWGG 2 cut(s) 54, 689
EcoT22I ATGCAT 1 cut(s) 451
ErhI CCWWGG 2 cut(s) 54, 689
Esp3I CGTCTC 2 cut(s) 175, 336
FaeI CATG 6 cut(s) 58, 118, 449, 666, 671, 693
FalI AAGNNNNNCTT 4 cut(s) 481, 513, 789, 821
FaqI GGGAC 1 cut(s) 846
FatI CATG 6 cut(s) 54, 114, 445, 662, 667, 689
Fnu4HI GCNGC 3 cut(s) 731, 870, 1083
FokI GGATG 4 cut(s) 17, 293, 298, 1088
Fsp4HI GCNGC 3 cut(s) 731, 870, 1083
FspBI CTAG 1 cut(s) 756
GlaI GCGC 2 cut(s) 703, 1057
GluI GCNGC 3 cut(s) 731, 870, 1083
GsuI CTGGAG 2 cut(s) 241, 955
HaeII RGCGCY 1 cut(s) 1059
HaeIII GGCC 1 cut(s) 612
HapII CCGG 3 cut(s) 299, 992, 1005
HhaI GCGC 2 cut(s) 704, 1058
Hin1II CATG 6 cut(s) 58, 118, 449, 666, 671, 693
Hin6I GCGC 2 cut(s) 702, 1056
HinP1I GCGC 2 cut(s) 702, 1056
HincII GTYRAC 1 cut(s) 517
HindII GTYRAC 1 cut(s) 517
HindIII AAGCTT 2 cut(s) 531, 898
HinfI GANTC 2 cut(s) 398, 1067
HpaII CCGG 3 cut(s) 299, 992, 1005
HphI GGTGA 3 cut(s) 949, 1006, 1027
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 5 cut(s) 121, 436, 487, 564, 891
Hpy188III TCNNGA 5 cut(s) 18, 299, 369, 382, 812
Hpy8I GTNNAC 1 cut(s) 517
HpyAV CCTTC 3 cut(s) 74, 379, 559
HpyCH4III ACNGT 2 cut(s) 330, 741
HpyCH4IV ACGT 3 cut(s) 184, 214, 303
HpyCH4V TGCA 8 cut(s) 8, 197, 211, 252, 449, 467, 548, 1093
HpyF10VI GCNNNNNNNGC 1 cut(s) 545
HpyF3I CTNAG 4 cut(s) 120, 484, 540, 614
HpySE526I ACGT 3 cut(s) 184, 214, 303
Hsp92II CATG 6 cut(s) 58, 118, 449, 666, 671, 693
HspAI GCGC 2 cut(s) 702, 1056
Kpn2I TCCGGA 1 cut(s) 298
Kzo9I GATC 4 cut(s) 109, 307, 374, 948
Lsp1109I GCAGC 3 cut(s) 742, 881, 1069
LweI GCATC 2 cut(s) 19, 23
MaeI CTAG 1 cut(s) 756
MaeII ACGT 3 cut(s) 184, 214, 303
MaeIII GTNAC 1 cut(s) 1015
MalI GATC 4 cut(s) 111, 309, 376, 950
MboI GATC 4 cut(s) 109, 307, 374, 948
MboII GAAGA 7 cut(s) 384, 653, 671, 797, 800, 803, 940
MfeI CAATTG 1 cut(s) 24
MluCI AATT 2 cut(s) 24, 247
MmeI TCCRAC 2 cut(s) 46, 675
MnlI CCTC 6 cut(s) 423, 623, 787, 901, 930, 933
Mph1103I ATGCAT 1 cut(s) 451
MroI TCCGGA 1 cut(s) 298
MroXI GAANNNNTTC 1 cut(s) 986
MseI TTAA 3 cut(s) 153, 165, 587
MslI CAYNNNNRTG 1 cut(s) 588
MspA1I CMGCKG 1 cut(s) 556
MspI CCGG 3 cut(s) 299, 992, 1005
MspR9I CCNGG 2 cut(s) 220, 992
MunI CAATTG 1 cut(s) 24
Mva1269I GAATGC 1 cut(s) 757
MvaI CCWGG 1 cut(s) 220
MvnI CGCG 1 cut(s) 813
MwoI GCNNNNNNNGC 1 cut(s) 545
NciI CCSGG 1 cut(s) 992
NcoI CCATGG 2 cut(s) 54, 689
NdeII GATC 4 cut(s) 109, 307, 374, 948
NlaIII CATG 6 cut(s) 58, 118, 449, 666, 671, 693
NlaIV GGNNCC 2 cut(s) 262, 862
NmuCI GTSAC 1 cut(s) 1015
NruI TCGCGA 1 cut(s) 813
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 2 cut(s) 449, 666
NspV TTCGAA 1 cut(s) 1038
PaeI GCATGC 1 cut(s) 666
PcsI WCGNNNNNNNCGW 1 cut(s) 190
PctI GAATGC 1 cut(s) 757
PdmI GAANNNNTTC 1 cut(s) 986
PfeI GAWTC 2 cut(s) 398, 1067
PflMI CCANNNNNTGG 1 cut(s) 966
PkrI GCNGC 3 cut(s) 732, 871, 1084
PshBI ATTAAT 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 218
PspGI CCWGG 1 cut(s) 218
PspN4I GGNNCC 2 cut(s) 262, 862
PspPI GGNCC 3 cut(s) 20, 686, 860
PvuII CAGCTG 1 cut(s) 556
RruI TCGCGA 1 cut(s) 813
RsaI GTAC 2 cut(s) 425, 444
RsaNI GTAC 2 cut(s) 424, 443
RseI CAYNNNNRTG 1 cut(s) 588
SaqAI TTAA 3 cut(s) 153, 165, 587
SatI GCNGC 3 cut(s) 731, 870, 1083
Sau3AI GATC 4 cut(s) 109, 307, 374, 948
Sau96I GGNCC 3 cut(s) 20, 686, 860
ScrFI CCNGG 2 cut(s) 220, 992
SfaNI GCATC 2 cut(s) 19, 23
SfuI TTCGAA 1 cut(s) 1038
SinI GGWCC 3 cut(s) 20, 686, 860
SmiMI CAYNNNNRTG 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 333
SmoI CTYRAG 1 cut(s) 333
SphI GCATGC 1 cut(s) 666
Sse9I AATT 2 cut(s) 24, 247
SsiI CCGC 1 cut(s) 1013
SspMI CTAG 1 cut(s) 756
StyD4I CCNGG 2 cut(s) 218, 990
StyI CCWWGG 2 cut(s) 54, 689
TaaI ACNGT 2 cut(s) 330, 741
TaiI ACGT 3 cut(s) 187, 217, 306
TaqI TCGA 2 cut(s) 428, 1038
TasI AATT 2 cut(s) 24, 247
TatI WGTACW 1 cut(s) 442
TfiI GAWTC 2 cut(s) 398, 1067
Tru1I TTAA 3 cut(s) 153, 165, 587
Tru9I TTAA 3 cut(s) 153, 165, 587
TscAI CASTG 2 cut(s) 259, 1077
TseFI GTSAC 1 cut(s) 1015
TseI GCWGC 3 cut(s) 730, 869, 1082
Tsp45I GTSAC 1 cut(s) 1015
TspDTI ATGAA 3 cut(s) 390, 492, 1092
TspRI CASTG 2 cut(s) 259, 1077
Van91I CCANNNNNTGG 1 cut(s) 966
VpaK11BI GGWCC 3 cut(s) 20, 686, 860
VspI ATTAAT 1 cut(s) 165
XceI RCATGY 2 cut(s) 449, 666
XcmI CCANNNNNNNNNTGG 1 cut(s) 30
XmnI GAANNNNTTC 1 cut(s) 986
XspI CTAG 1 cut(s) 756
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.