FvH4_5g23700

Isoflavone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001108
Physical Location & Seq
Reverse (-)
15039638 .. 15043797
4160 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g23700.t1

Sequence Viewer

Length: 951 bp
ATGGCAACTACAAAGAGCAAGGTTCTTGTGGTTGGTGGAACTGGTTACATGGGGAGGAGGATTGTCAAGGCAAGCCTAGCAGCAGGCCACCCGACTTATGTTCTTCAAAGGCCGGCGCCGGAGATCGGCCTCGACATTGAGAAGCTACAAATGCTCTTCTCGTTTAAGAAGCAAGGCGCTCATCTCGTCGAGGGTTCGTTTTCAGATTTCCACAGCCTTGTTGATGCTGTCAAGCTAGTTGATGTTGTCATATGCACCATGTCGGGGACACATATCCGGAGTCACAACATTATGCTGCAGCTCAAACTTGTTGAAGCCATTAAAGAAGCTGGAAATGTGAAGCGTTTCTTGCCATCGGAGTTTGGTATGGACGTAGCACGTATGGGAAATGCTCTTGAACCGGGAAGAGTTCCCTTTGATGACAAAATGAAAGTGAGAAAAGCAATTGAAGATGCTAACATCCCCTTCACATATGTATGTGGTATTGGCTTCGCAGCTTACCATGCTGGAAACCTTTGCCAAATGGGAACCCTGGTTCCTCCAAGGGATAAGGTGCTTATGTATGGAGATGGCGACCGAAAAGTGATACTTATGGATGAGGATGACATTGCAACATACGCGATAAAAACAATTGATGATCCGCGAACATTTAACAAAACATTGCACCTTCGTCCTTTGGAGTGCATAGTCTCTCAAAGACAGTTGGTGGAGATCTGGGAAAGCCTTGTTGGGAAGAAGCTAGAAAAGGTTACCCTTTCCAAAGAAGACTTCCTTGTCTCTATGAAAGATATGGACTATGCAAATCAAGTAGGAGCAGGACATTTCCATGACATGTACTACGAAGGTTGTTTGACAAACTTTGAAATAGGGGAAAATGAAGAAGAAGCTTCAAAGCTTTATCCCGAAGTGAAGTACACTCGCATGCATGAATTTTTGAATATTTATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

317

Amino Acids

35.51

Weight (kDa)

6.22

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 4 - 312 1.6e-58 NmrA-like family
Epimerase PF01370 8 - 117 2.5e-06 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 12 - 160 3.9e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 773
AccB1I GGYRCC 1 cut(s) 115
AccII CGCG 2 cut(s) 620, 643
AccIII TCCGGA 1 cut(s) 276
AciI CCGC 1 cut(s) 641
AclWI GGATC 1 cut(s) 632
AcsI RAATTY 1 cut(s) 929
AcyI GRCGYC 1 cut(s) 116
AfaI GTAC 2 cut(s) 836, 914
AfiI CCNNNNNNNGG 2 cut(s) 125, 264
AflIII ACRYGT 1 cut(s) 831
AgsI TTSAA 7 cut(s) 107, 314, 398, 449, 863, 891, 937
AjnI CCWGG 1 cut(s) 531
AjuI GAANNNNNNNTTGG 4 cut(s) 711, 743, 752, 784
AloI GAACNNNNNNTCC 4 cut(s) 519, 520, 551, 552
AluBI AGCT 8 cut(s) 145, 235, 301, 329, 497, 739, 887, 895
AluI AGCT 8 cut(s) 145, 235, 301, 329, 497, 739, 887, 895
Alw26I GTCTC 2 cut(s) 694, 781
AlwI GGATC 1 cut(s) 632
Aor13HI TCCGGA 1 cut(s) 276
AoxI GGCC 3 cut(s) 85, 110, 127
ApeKI GCWGC 4 cut(s) 80, 295, 298, 494
ApoI RAATTY 1 cut(s) 929
Asp700I GAANNNNTTC 1 cut(s) 344
AspLEI GCGC 2 cut(s) 118, 179
AsuC2I CCSGG 1 cut(s) 402
BanI GGYRCC 1 cut(s) 115
BbsI GAAGAC 1 cut(s) 771
BbvI GCAGC 4 cut(s) 92, 282, 310, 506
BccI CCATC 2 cut(s) 361, 563
BciT130I CCWGG 1 cut(s) 533
BcnI CCSGG 1 cut(s) 402
BcoDI GTCTC 2 cut(s) 694, 781
BfaI CTAG 3 cut(s) 77, 236, 740
BfmI CTRYAG 1 cut(s) 296
BfoI RGCGCY 2 cut(s) 119, 180
BglII AGATCT 1 cut(s) 711
BisI GCNGC 4 cut(s) 81, 296, 299, 495
BlsI GCNGC 4 cut(s) 82, 297, 300, 496
Bme1390I CCNGG 2 cut(s) 402, 533
BmiI GGNNCC 3 cut(s) 117, 529, 537
BmrFI CCNGG 2 cut(s) 402, 533
BmsI GCATC 2 cut(s) 214, 442
BpiI GAAGAC 1 cut(s) 771
BpuMI CCSGG 1 cut(s) 402
BsaAI YACGTR 1 cut(s) 380
BsaHI GRCGYC 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 531, 542
BsaWI WCCGGW 1 cut(s) 276
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 264
Bse118I RCCGGY 1 cut(s) 112
Bse1I ACTGG 1 cut(s) 46
Bse3DI GCAATG 2 cut(s) 606, 659
BseAI TCCGGA 1 cut(s) 276
BseBI CCWGG 1 cut(s) 533
BseDI CCNNGG 2 cut(s) 531, 542
BseGI GGATG 3 cut(s) 459, 601, 607
BseLI CCNNNNNNNGG 2 cut(s) 125, 264
BseMI GCAATG 2 cut(s) 606, 659
BseNI ACTGG 1 cut(s) 46
BseRI GAGGAG 1 cut(s) 70
BseXI GCAGC 4 cut(s) 92, 282, 310, 506
Bsh1236I CGCG 2 cut(s) 620, 643
Bsh1285I CGRYCG 1 cut(s) 577
BshFI GGCC 3 cut(s) 87, 112, 129
BshNI GGYRCC 1 cut(s) 115
BsiEI CGRYCG 1 cut(s) 577
BsiSI CCGG 4 cut(s) 113, 119, 277, 401
BslFI GGGAC 1 cut(s) 280
BslI CCNNNNNNNGG 2 cut(s) 125, 264
BsmAI GTCTC 2 cut(s) 694, 781
BsmFI GGGAC 1 cut(s) 280
BsnI GGCC 3 cut(s) 87, 112, 129
Bsp13I TCCGGA 1 cut(s) 276
Bsp143I GATC 3 cut(s) 123, 637, 711
BspACI CCGC 1 cut(s) 641
BspANI GGCC 3 cut(s) 87, 112, 129
BspEI TCCGGA 1 cut(s) 276
BspFNI CGCG 2 cut(s) 620, 643
BspLI GGNNCC 3 cut(s) 117, 529, 537
BspMAI CTGCAG 1 cut(s) 300
BspPI GGATC 1 cut(s) 632
BspQI GCTCTTC 1 cut(s) 161
BspT107I GGYRCC 1 cut(s) 115
BsrDI GCAATG 2 cut(s) 606, 659
BsrFI RCCGGY 1 cut(s) 112
BsrI ACTGG 1 cut(s) 46
BssAI RCCGGY 1 cut(s) 112
BssECI CCNNGG 2 cut(s) 531, 542
BssMI GATC 3 cut(s) 123, 637, 711
BssNI GRCGYC 1 cut(s) 116
BssT1I CCWWGG 1 cut(s) 542
Bst2UI CCWGG 1 cut(s) 533
Bst4CI ACNGT 1 cut(s) 702
Bst6I CTCTTC 2 cut(s) 161, 400
BstACI GRCGYC 1 cut(s) 116
BstBAI YACGTR 1 cut(s) 380
BstC8I GCNNGC 4 cut(s) 73, 85, 114, 923
BstEII GGTNACC 1 cut(s) 748
BstF5I GGATG 3 cut(s) 459, 601, 607
BstFNI CGCG 2 cut(s) 620, 643
BstH2I RGCGCY 2 cut(s) 119, 180
BstHHI GCGC 2 cut(s) 118, 179
BstKTI GATC 3 cut(s) 126, 640, 714
BstMAI GTCTC 2 cut(s) 694, 781
BstMBI GATC 3 cut(s) 123, 637, 711
BstMCI CGRYCG 1 cut(s) 577
BstMWI GCNNNNNNNGC 5 cut(s) 77, 151, 349, 503, 617
BstNI CCWGG 1 cut(s) 533
BstNSI RCATGY 2 cut(s) 835, 925
BstPI GGTNACC 1 cut(s) 748
BstSCI CCNGG 2 cut(s) 400, 531
BstSFI CTRYAG 1 cut(s) 296
BstUI CGCG 2 cut(s) 620, 643
BstV1I GCAGC 4 cut(s) 92, 282, 310, 506
BstV2I GAAGAC 1 cut(s) 771
BstX2I RGATCY 1 cut(s) 711
BstYI RGATCY 1 cut(s) 711
BsuRI GGCC 3 cut(s) 87, 112, 129
BtsCI GGATG 3 cut(s) 459, 601, 607
Cac8I GCNNGC 4 cut(s) 73, 85, 114, 923
CfoI GCGC 2 cut(s) 118, 179
Cfr10I RCCGGY 1 cut(s) 112
Csp6I GTAC 2 cut(s) 835, 913
CviAII CATG 7 cut(s) 49, 259, 503, 827, 832, 922, 926
CviQI GTAC 2 cut(s) 835, 913
DinI GGCGCC 1 cut(s) 117
DpnI GATC 3 cut(s) 125, 639, 713
DpnII GATC 3 cut(s) 123, 637, 711
DrdI GACNNNNNNGTC 1 cut(s) 773
DseDI GACNNNNNNGTC 1 cut(s) 773
Eam1104I CTCTTC 2 cut(s) 161, 400
EarI CTCTTC 2 cut(s) 161, 400
Eco130I CCWWGG 1 cut(s) 542
Eco91I GGTNACC 1 cut(s) 748
EcoO65I GGTNACC 1 cut(s) 748
EcoRII CCWGG 1 cut(s) 531
EcoT14I CCWWGG 1 cut(s) 542
EcoT22I ATGCAT 1 cut(s) 927
EgeI GGCGCC 1 cut(s) 117
EheI GGCGCC 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 542
FaeI CATG 7 cut(s) 52, 262, 506, 830, 835, 925, 929
FalI AAGNNNNNCTT 6 cut(s) 332, 364, 573, 605, 756, 788
FaqI GGGAC 1 cut(s) 280
FatI CATG 7 cut(s) 48, 258, 502, 826, 831, 921, 925
FauNDI CATATG 2 cut(s) 251, 472
Fnu4HI GCNGC 4 cut(s) 81, 296, 299, 495
FokI GGATG 3 cut(s) 446, 608, 614
Fsp4HI GCNGC 4 cut(s) 81, 296, 299, 495
FspBI CTAG 3 cut(s) 77, 236, 740
GlaI GCGC 2 cut(s) 117, 178
GluI GCNGC 4 cut(s) 81, 296, 299, 495
HaeII RGCGCY 2 cut(s) 119, 180
HaeIII GGCC 3 cut(s) 87, 112, 129
HapII CCGG 4 cut(s) 113, 119, 277, 401
HhaI GCGC 2 cut(s) 118, 179
Hin1I GRCGYC 1 cut(s) 116
Hin1II CATG 7 cut(s) 52, 262, 506, 830, 835, 925, 929
Hin6I GCGC 2 cut(s) 116, 177
HinP1I GCGC 2 cut(s) 116, 177
HindIII AAGCTT 2 cut(s) 885, 893
HinfI GANTC 1 cut(s) 280
HpaII CCGG 4 cut(s) 113, 119, 277, 401
Hpy166II GTNNAC 1 cut(s) 915
Hpy188I TCNGA 2 cut(s) 205, 358
Hpy188III TCNNGA 3 cut(s) 277, 395, 902
Hpy8I GTNNAC 1 cut(s) 915
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 3 cut(s) 475, 677, 836
HpyCH4III ACNGT 1 cut(s) 702
HpyCH4IV ACGT 2 cut(s) 372, 379
HpyCH4V TGCA 7 cut(s) 255, 298, 611, 664, 684, 800, 925
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 151, 349, 503, 617
HpySE526I ACGT 2 cut(s) 372, 379
Hsp92I GRCGYC 1 cut(s) 116
Hsp92II CATG 7 cut(s) 52, 262, 506, 830, 835, 925, 929
HspAI GCGC 2 cut(s) 116, 177
KasI GGCGCC 1 cut(s) 115
Kpn2I TCCGGA 1 cut(s) 276
KroI GCCGGC 1 cut(s) 112
KroNI GCCGGC 1 cut(s) 114
Kzo9I GATC 3 cut(s) 123, 637, 711
LguI GCTCTTC 1 cut(s) 161
LmnI GCTCC 1 cut(s) 812
Lsp1109I GCAGC 4 cut(s) 92, 282, 310, 506
LweI GCATC 2 cut(s) 214, 442
MaeI CTAG 3 cut(s) 77, 236, 740
MaeII ACGT 2 cut(s) 372, 379
MaeIII GTNAC 3 cut(s) 44, 281, 748
MalI GATC 3 cut(s) 125, 639, 713
MboI GATC 3 cut(s) 123, 637, 711
MboII GAAGA 8 cut(s) 95, 148, 417, 461, 745, 776, 890, 893
MfeI CAATTG 2 cut(s) 444, 630
MflI RGATCY 1 cut(s) 711
MluCI AATT 3 cut(s) 444, 630, 929
Mly113I GGCGCC 1 cut(s) 116
MlyI GAGTC 1 cut(s) 289
MnlI CCTC 6 cut(s) 48, 51, 140, 184, 549, 592
Mph1103I ATGCAT 1 cut(s) 927
MroI TCCGGA 1 cut(s) 276
MroNI GCCGGC 1 cut(s) 112
MroXI GAANNNNTTC 1 cut(s) 344
MseI TTAA 3 cut(s) 165, 321, 651
MslI CAYNNNNRTG 3 cut(s) 475, 825, 920
MspI CCGG 4 cut(s) 113, 119, 277, 401
MspR9I CCNGG 2 cut(s) 402, 533
MunI CAATTG 2 cut(s) 444, 630
MvaI CCWGG 1 cut(s) 533
MvnI CGCG 2 cut(s) 620, 643
MwoI GCNNNNNNNGC 5 cut(s) 77, 151, 349, 503, 617
NaeI GCCGGC 1 cut(s) 114
NarI GGCGCC 1 cut(s) 116
NciI CCSGG 1 cut(s) 402
NdeI CATATG 2 cut(s) 251, 472
NdeII GATC 3 cut(s) 123, 637, 711
NgoMIV GCCGGC 1 cut(s) 112
NlaIII CATG 7 cut(s) 52, 262, 506, 830, 835, 925, 929
NlaIV GGNNCC 3 cut(s) 117, 529, 537
NmuCI GTSAC 1 cut(s) 281
NsiI ATGCAT 1 cut(s) 927
NspI RCATGY 2 cut(s) 835, 925
PaeI GCATGC 1 cut(s) 925
PciI ACATGT 1 cut(s) 831
PciSI GCTCTTC 1 cut(s) 161
PdiI GCCGGC 1 cut(s) 114
PdmI GAANNNNTTC 1 cut(s) 344
PkrI GCNGC 4 cut(s) 82, 297, 300, 496
PleI GAGTC 1 cut(s) 288
PluTI GGCGCC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 288
Ppu21I YACGTR 1 cut(s) 380
PscI ACATGT 1 cut(s) 831
Psp6I CCWGG 1 cut(s) 531
PspEI GGTNACC 1 cut(s) 748
PspGI CCWGG 1 cut(s) 531
PspN4I GGNNCC 3 cut(s) 117, 529, 537
PstI CTGCAG 1 cut(s) 300
PsuI RGATCY 1 cut(s) 711
RsaI GTAC 2 cut(s) 836, 914
RsaNI GTAC 2 cut(s) 835, 913
RseI CAYNNNNRTG 3 cut(s) 475, 825, 920
SapI GCTCTTC 1 cut(s) 161
SaqAI TTAA 3 cut(s) 165, 321, 651
SatI GCNGC 4 cut(s) 81, 296, 299, 495
Sau3AI GATC 3 cut(s) 123, 637, 711
SchI GAGTC 1 cut(s) 289
ScrFI CCNGG 2 cut(s) 402, 533
SfaNI GCATC 2 cut(s) 214, 442
SfcI CTRYAG 1 cut(s) 296
SfoI GGCGCC 1 cut(s) 117
SmiMI CAYNNNNRTG 3 cut(s) 475, 825, 920
SphI GCATGC 1 cut(s) 925
Sse9I AATT 3 cut(s) 444, 630, 929
SsiI CCGC 1 cut(s) 641
SspDI GGCGCC 1 cut(s) 115
SspI AATATT 1 cut(s) 940
SspMI CTAG 3 cut(s) 77, 236, 740
StyD4I CCNGG 2 cut(s) 400, 531
StyI CCWWGG 1 cut(s) 542
TaaI ACNGT 1 cut(s) 702
TaiI ACGT 2 cut(s) 375, 382
TaqI TCGA 2 cut(s) 132, 189
TaqII GACCGA 1 cut(s) 591
TasI AATT 3 cut(s) 444, 630, 929
TatI WGTACW 2 cut(s) 834, 912
Tru1I TTAA 3 cut(s) 165, 321, 651
Tru9I TTAA 3 cut(s) 165, 321, 651
TseFI GTSAC 1 cut(s) 281
TseI GCWGC 4 cut(s) 80, 295, 298, 494
Tsp45I GTSAC 1 cut(s) 281
TspDTI ATGAA 4 cut(s) 443, 797, 891, 942
XapI RAATTY 1 cut(s) 929
XceI RCATGY 2 cut(s) 835, 925
XmnI GAANNNNTTC 1 cut(s) 344
XspI CTAG 3 cut(s) 77, 236, 740
Zsp2I ATGCAT 1 cut(s) 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.