Rh7BG265900

Isoflavone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001108
Physical Location & Seq
Reverse (-)
24338554 .. 24342195
3642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG265900.1

Sequence Viewer

Length: 1056 bp
ATGGACATCGATTGCCAACTACACTGTCTCTCTCTATATATATATAGGTATCGTGTCTTTGTAATAACGGAAGACATAACCAGAGAGCTAGGCAAAGACATGGCAAATGCAAAGAGCAACCTTAAGGTTCTTGTGGTAGGTGGTACTGGATATGTTGGGAGAAGGATTGTTAGTGCAAGCCTAGCACAAGGCTACCCGACTTATGTCCTTCAAAGGCCGGCAACGGAGATTGGCCTCGATATTGAGAAACTACAAATGCTCTTGTCGTTTAAGAAGCAAGGGGCTCATCTCGTGGAGGGTTCGTTTTCTGATATCCAAAGCCTTGTCGATGCCGTGAAACAAGTTGACGTTGTCATTTGCACCATGTCGGGGGTGCATTTCCGGAGTCACAACCTTTTGTTGCAGCTCAAGCTTGTTGAAGCCATTAAACAAGCTGGAAATGTTAAGCGCTTCTTGCCATCAGAGTTTGGTTTGGACCCGGCGCGTATGGGACATGCACTTGAACCAGGAAGAGTAACATTTGATGAGAAAATGGTGGTGAGAAAGGCAATAGAAGACGCCAACATCCCCTTCACCTATGTGTGTGGTGCTGGCTTCGCCGGTTATTTTGCGGGCAATCTCTCTCAGATGGGAACACTTGTTCCTCCGAAGGACAAAGTCCTTATTTATGGAGATGGCAACACTAAAGTATCTTTCATGGACGAAGATGACATCGGAAGCTATACAATCAAGACAATAGATGATCCGCGAACTTGCAACAAAACATTGTATCTTCTTCCGCCCGAAAACCTACTCACTCAAAGACAATTGGTGGACATTTGGGAAAATCTTGTTGGAAAGAAACTAGAGAAAATTTCCCTTTCTAAAGAAGACCTCCTTGCCTCTATGAAAGGTATGGACTATGCAGGCCAGGTCGGGGTGGGGCATTTCTATCACATTTTCTATGAAGGCTCTTTGACAAACTTCGAAATAGAGGAGGAAGGAGAAGAAGCTTCAAAGCTTTATCCAGAAGTGAAATACACCCGCATGGATGAATACTTGAAAATTTATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

351

Amino Acids

39.34

Weight (kDa)

5.61

Isoelectric Point (pI)

36.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 42 - 347 6.7e-65 NmrA-like family
Epimerase PF01370 43 - 152 2.4e-08 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 47 - 196 3.5e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 484, 748
AccIII TCCGGA 1 cut(s) 381
AciI CCGC 4 cut(s) 611, 746, 779, 1024
AclWI GGATC 1 cut(s) 737
AcsI RAATTY 2 cut(s) 852, 1044
AcyI GRCGYC 1 cut(s) 558
AfaI GTAC 1 cut(s) 145
AfeI AGCGCT 1 cut(s) 449
AfiI CCNNNNNNNGG 2 cut(s) 369, 916
AflII CTTAAG 1 cut(s) 122
AgsI TTSAA 5 cut(s) 212, 419, 503, 996, 1042
AjnI CCWGG 2 cut(s) 505, 909
AjuI GAANNNNNNNTTGG 4 cut(s) 554, 586, 816, 848
AleI CACNNNNGTG 1 cut(s) 578
AloI GAACNNNNNNTCC 4 cut(s) 624, 625, 656, 657
AluBI AGCT 7 cut(s) 88, 406, 412, 434, 720, 992, 1000
AluI AGCT 7 cut(s) 88, 406, 412, 434, 720, 992, 1000
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 1 cut(s) 737
Aor13HI TCCGGA 1 cut(s) 381
Aor51HI AGCGCT 1 cut(s) 449
AoxI GGCC 3 cut(s) 215, 232, 907
ApeKI GCWGC 1 cut(s) 403
ApoI RAATTY 2 cut(s) 852, 1044
AspLEI GCGC 2 cut(s) 450, 484
AspS9I GGNCC 1 cut(s) 475
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 2 cut(s) 550, 565
AsuII TTCGAA 1 cut(s) 966
AvaII GGWCC 1 cut(s) 475
BaeI ACNNNNGTAYC 2 cut(s) 672, 705
BanII GRGCYC 1 cut(s) 286
BauI CACGAG 1 cut(s) 290
BbsI GAAGAC 3 cut(s) 78, 561, 876
BbvI GCAGC 1 cut(s) 415
BccI CCATC 3 cut(s) 466, 622, 668
BceAI ACGGC 1 cut(s) 317
BciT130I CCWGG 2 cut(s) 507, 911
BcnI CCSGG 1 cut(s) 479
BcoDI GTCTC 1 cut(s) 32
BfaI CTAG 3 cut(s) 89, 182, 845
BfoI RGCGCY 1 cut(s) 451
BfrI CTTAAG 1 cut(s) 122
BisI GCNGC 1 cut(s) 404
BlsI GCNGC 1 cut(s) 405
Bme1390I CCNGG 3 cut(s) 479, 507, 911
Bme18I GGWCC 1 cut(s) 475
BmgT120I GGNCC 1 cut(s) 475
BmiI GGNNCC 1 cut(s) 477
BmrFI CCNGG 3 cut(s) 479, 507, 911
BmsI GCATC 1 cut(s) 319
BoxI GACNNNNGTC 1 cut(s) 203
BpiI GAAGAC 3 cut(s) 78, 561, 876
Bpu14I TTCGAA 1 cut(s) 966
BpuEI CTTGAG 1 cut(s) 392
BpuMI CCSGG 1 cut(s) 479
Bsa29I ATCGAT 1 cut(s) 9
BsaHI GRCGYC 1 cut(s) 558
BsaWI WCCGGW 1 cut(s) 381
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 2 cut(s) 369, 916
Bse118I RCCGGY 2 cut(s) 217, 599
Bse1I ACTGG 1 cut(s) 151
BseAI TCCGGA 1 cut(s) 381
BseBI CCWGG 2 cut(s) 507, 911
BseCI ATCGAT 1 cut(s) 9
BseGI GGATG 2 cut(s) 564, 1036
BseLI CCNNNNNNNGG 2 cut(s) 369, 916
BseMII CTCAG 1 cut(s) 638
BseNI ACTGG 1 cut(s) 151
BseRI GAGGAG 1 cut(s) 989
BseXI GCAGC 1 cut(s) 415
Bsh1236I CGCG 2 cut(s) 484, 748
BshFI GGCC 3 cut(s) 217, 234, 909
BshVI ATCGAT 1 cut(s) 9
BsiSI CCGG 4 cut(s) 218, 382, 479, 600
BslFI GGGAC 1 cut(s) 504
BslI CCNNNNNNNGG 2 cut(s) 369, 916
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 504
BsnI GGCC 3 cut(s) 217, 234, 909
Bsp119I TTCGAA 1 cut(s) 966
Bsp1286I GDGCHC 1 cut(s) 286
Bsp13I TCCGGA 1 cut(s) 381
Bsp143I GATC 1 cut(s) 742
BspACI CCGC 4 cut(s) 611, 746, 779, 1024
BspANI GGCC 3 cut(s) 217, 234, 909
BspCNI CTCAG 1 cut(s) 637
BspDI ATCGAT 1 cut(s) 9
BspEI TCCGGA 1 cut(s) 381
BspFNI CGCG 2 cut(s) 484, 748
BspLI GGNNCC 1 cut(s) 477
BspPI GGATC 1 cut(s) 737
BspT104I TTCGAA 1 cut(s) 966
BspTI CTTAAG 1 cut(s) 122
BsrFI RCCGGY 2 cut(s) 217, 599
BsrI ACTGG 1 cut(s) 151
BssAI RCCGGY 2 cut(s) 217, 599
BssMI GATC 1 cut(s) 742
BssNI GRCGYC 1 cut(s) 558
BssSI CACGAG 1 cut(s) 290
Bst2BI CACGAG 1 cut(s) 290
Bst2UI CCWGG 2 cut(s) 507, 911
Bst4CI ACNGT 1 cut(s) 26
Bst6I CTCTTC 1 cut(s) 505
BstACI GRCGYC 1 cut(s) 558
BstAFI CTTAAG 1 cut(s) 122
BstBI TTCGAA 1 cut(s) 966
BstC8I GCNNGC 5 cut(s) 178, 219, 592, 613, 907
BstDEI CTNAG 1 cut(s) 624
BstF5I GGATG 2 cut(s) 564, 1036
BstFNI CGCG 2 cut(s) 484, 748
BstH2I RGCGCY 1 cut(s) 451
BstHHI GCGC 2 cut(s) 450, 484
BstKTI GATC 1 cut(s) 745
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 1 cut(s) 742
BstMWI GCNNNNNNNGC 4 cut(s) 182, 409, 454, 596
BstNI CCWGG 2 cut(s) 507, 911
BstNSI RCATGY 1 cut(s) 497
BstPAI GACNNNNGTC 1 cut(s) 203
BstSCI CCNGG 3 cut(s) 477, 505, 909
BstUI CGCG 2 cut(s) 484, 748
BstV1I GCAGC 1 cut(s) 415
BstV2I GAAGAC 3 cut(s) 78, 561, 876
Bsu15I ATCGAT 1 cut(s) 9
BsuRI GGCC 3 cut(s) 217, 234, 909
BsuTUI ATCGAT 1 cut(s) 9
BtsCI GGATG 2 cut(s) 564, 1036
BtsIMutI CAGTG 1 cut(s) 22
Cac8I GCNNGC 5 cut(s) 178, 219, 592, 613, 907
CfoI GCGC 2 cut(s) 450, 484
Cfr10I RCCGGY 2 cut(s) 217, 599
Cfr13I GGNCC 1 cut(s) 475
ClaI ATCGAT 1 cut(s) 9
CseI GACGC 1 cut(s) 566
Csp6I GTAC 1 cut(s) 144
CviAII CATG 5 cut(s) 100, 364, 494, 697, 1027
CviQI GTAC 1 cut(s) 144
DdeI CTNAG 1 cut(s) 624
DpnI GATC 1 cut(s) 744
DpnII GATC 1 cut(s) 742
Eam1104I CTCTTC 1 cut(s) 505
EarI CTCTTC 1 cut(s) 505
EciI GGCGGA 1 cut(s) 768
Eco24I GRGCYC 1 cut(s) 286
Eco32I GATATC 1 cut(s) 313
Eco47I GGWCC 1 cut(s) 475
Eco47III AGCGCT 1 cut(s) 449
EcoRII CCWGG 2 cut(s) 505, 909
EcoRV GATATC 1 cut(s) 313
EcoT22I ATGCAT 1 cut(s) 1054
EcoT38I GRGCYC 1 cut(s) 286
FaeI CATG 5 cut(s) 103, 367, 497, 700, 1030
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 861, 893
FaqI GGGAC 1 cut(s) 504
FatI CATG 5 cut(s) 99, 363, 493, 696, 1026
FauI CCCGC 2 cut(s) 604, 1031
Fnu4HI GCNGC 1 cut(s) 404
FokI GGATG 2 cut(s) 551, 1043
FriOI GRGCYC 1 cut(s) 286
Fsp4HI GCNGC 1 cut(s) 404
FspBI CTAG 3 cut(s) 89, 182, 845
GlaI GCGC 2 cut(s) 449, 483
GluI GCNGC 1 cut(s) 404
HaeII RGCGCY 1 cut(s) 451
HaeIII GGCC 3 cut(s) 217, 234, 909
HapII CCGG 4 cut(s) 218, 382, 479, 600
HgaI GACGC 1 cut(s) 566
HhaI GCGC 2 cut(s) 450, 484
Hin1I GRCGYC 1 cut(s) 558
Hin1II CATG 5 cut(s) 103, 367, 497, 700, 1030
Hin6I GCGC 2 cut(s) 448, 482
HinP1I GCGC 2 cut(s) 448, 482
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HindIII AAGCTT 3 cut(s) 410, 990, 998
HinfI GANTC 1 cut(s) 385
HpaII CCGG 4 cut(s) 218, 382, 479, 600
HphI GGTGA 2 cut(s) 550, 565
Hpy166II GTNNAC 2 cut(s) 346, 814
Hpy188I TCNGA 5 cut(s) 310, 463, 627, 648, 716
Hpy188III TCNNGA 3 cut(s) 382, 730, 1007
Hpy8I GTNNAC 2 cut(s) 346, 814
HpyAV CCTTC 6 cut(s) 156, 218, 580, 643, 941, 974
HpyCH4III ACNGT 1 cut(s) 26
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 9 cut(s) 110, 176, 360, 376, 403, 497, 756, 905, 1052
HpyF10VI GCNNNNNNNGC 4 cut(s) 182, 409, 454, 596
HpyF3I CTNAG 1 cut(s) 624
HpySE526I ACGT 1 cut(s) 348
Hsp92I GRCGYC 1 cut(s) 558
Hsp92II CATG 5 cut(s) 103, 367, 497, 700, 1030
HspAI GCGC 2 cut(s) 448, 482
Kpn2I TCCGGA 1 cut(s) 381
KroI GCCGGC 1 cut(s) 217
KroNI GCCGGC 1 cut(s) 219
Kzo9I GATC 1 cut(s) 742
Lsp1109I GCAGC 1 cut(s) 415
LweI GCATC 1 cut(s) 319
MaeI CTAG 3 cut(s) 89, 182, 845
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 2 cut(s) 386, 514
MalI GATC 1 cut(s) 744
MboI GATC 1 cut(s) 742
MboII GAAGA 8 cut(s) 83, 522, 566, 716, 764, 767, 881, 998
MfeI CAATTG 1 cut(s) 806
MhlI GDGCHC 1 cut(s) 286
MluCI AATT 3 cut(s) 806, 852, 1044
MlyI GAGTC 1 cut(s) 394
MmeI TCCRAC 1 cut(s) 814
MnlI CCTC 7 cut(s) 245, 289, 654, 884, 892, 967, 970
Mph1103I ATGCAT 1 cut(s) 1054
MroI TCCGGA 1 cut(s) 381
MroNI GCCGGC 1 cut(s) 217
MseI TTAA 4 cut(s) 123, 270, 426, 444
MslI CAYNNNNRTG 2 cut(s) 578, 1025
MspCI CTTAAG 1 cut(s) 122
MspI CCGG 4 cut(s) 218, 382, 479, 600
MspR9I CCNGG 3 cut(s) 479, 507, 911
MunI CAATTG 1 cut(s) 806
MvaI CCWGG 2 cut(s) 507, 911
MvnI CGCG 2 cut(s) 484, 748
MwoI GCNNNNNNNGC 4 cut(s) 182, 409, 454, 596
NaeI GCCGGC 1 cut(s) 219
NciI CCSGG 1 cut(s) 479
NdeII GATC 1 cut(s) 742
NgoMIV GCCGGC 1 cut(s) 217
NlaIII CATG 5 cut(s) 103, 367, 497, 700, 1030
NlaIV GGNNCC 1 cut(s) 477
NmuCI GTSAC 1 cut(s) 386
NsiI ATGCAT 1 cut(s) 1054
NspI RCATGY 1 cut(s) 497
NspV TTCGAA 1 cut(s) 966
OliI CACNNNNGTG 1 cut(s) 578
PdiI GCCGGC 1 cut(s) 219
PflFI GACNNNGTC 2 cut(s) 350, 656
PkrI GCNGC 1 cut(s) 405
PleI GAGTC 1 cut(s) 393
PpsI GAGTC 1 cut(s) 393
PshAI GACNNNNGTC 1 cut(s) 203
Psp6I CCWGG 2 cut(s) 505, 909
PspGI CCWGG 2 cut(s) 505, 909
PspN4I GGNNCC 1 cut(s) 477
PspPI GGNCC 1 cut(s) 475
PsyI GACNNNGTC 2 cut(s) 350, 656
RsaI GTAC 1 cut(s) 145
RsaNI GTAC 1 cut(s) 144
RseI CAYNNNNRTG 2 cut(s) 578, 1025
SaqAI TTAA 4 cut(s) 123, 270, 426, 444
SatI GCNGC 1 cut(s) 404
Sau3AI GATC 1 cut(s) 742
Sau96I GGNCC 1 cut(s) 475
SchI GAGTC 1 cut(s) 394
ScrFI CCNGG 3 cut(s) 479, 507, 911
SduI GDGCHC 1 cut(s) 286
SfaNI GCATC 1 cut(s) 319
SfuI TTCGAA 1 cut(s) 966
SinI GGWCC 1 cut(s) 475
SmiMI CAYNNNNRTG 2 cut(s) 578, 1025
SmlI CTYRAG 2 cut(s) 122, 407
SmoI CTYRAG 2 cut(s) 122, 407
Sse9I AATT 3 cut(s) 806, 852, 1044
SsiI CCGC 4 cut(s) 611, 746, 779, 1024
SspMI CTAG 3 cut(s) 89, 182, 845
StyD4I CCNGG 3 cut(s) 477, 505, 909
TaaI ACNGT 1 cut(s) 26
TaiI ACGT 1 cut(s) 351
TaqI TCGA 4 cut(s) 9, 237, 327, 966
TasI AATT 3 cut(s) 806, 852, 1044
Tru1I TTAA 4 cut(s) 123, 270, 426, 444
Tru9I TTAA 4 cut(s) 123, 270, 426, 444
TscAI CASTG 1 cut(s) 29
TseFI GTSAC 1 cut(s) 386
TseI GCWGC 1 cut(s) 403
Tsp45I GTSAC 1 cut(s) 386
TspDTI ATGAA 4 cut(s) 685, 902, 960, 1047
TspGWI ACGGA 2 cut(s) 83, 239
TspRI CASTG 1 cut(s) 29
Tth111I GACNNNGTC 2 cut(s) 350, 656
Vha464I CTTAAG 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 475
XapI RAATTY 2 cut(s) 852, 1044
XceI RCATGY 1 cut(s) 497
XspI CTAG 3 cut(s) 89, 182, 845
Zsp2I ATGCAT 1 cut(s) 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.