Rorug07G0138500

Isoflavone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001108
Physical Location & Seq
Forward (+)
10929708 .. 10930247
540 bp
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UTR
Exon/CDS
Intron
Rorug07G0138500.1

Sequence Viewer

Length: 540 bp
ATGTGTGTGCCTAAGAGTGAAGGAGGCATGGGTTTTAGGAACTTGGTCTCTTTCAATCTGTCACTTCTTGCGAAACAAGGGTGGCGTTTACTAACCAAGCCGGATTCCATTGCAGCTAGACTATTTAAAGCAAAATATTTTCCAAACTCGGATTTTCTGCATGCTGAGGCTGGACAGGACATTTCATTTACGTGGCGCAGCATCCTGGCAGGTAGAGATGTGTTGGCTAGGGGGCTGAGAGTGCAGATTGGGGATGGTAGAGATGTGTCGGTGTGGGGGGATGCATGGCTACCGCTACCTTATAGTTTCAAGCCATTCTCATCACCTATGGAAGGTACAGAAGACTGGAGGGTTTGTGACTTGATAGATCAAGATCAGAAGGAGTGGATTCTGCCGGTGATTGAGGAACTGTTTACTGATGTTGAGGTGGCGATAATACAACGTATACCTCTTAGTATACGTCCTGCAGCGGACCGATTTGTTTGGCATTATGACAAGAAGGGTGTATTCAGTGTTAAGGCTAGGGTGGCTATGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

179

Amino Acids

20.38

Weight (kDa)

7.76

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 200
AccI GTMKAC 2 cut(s) 445, 457
AciI CCGC 2 cut(s) 293, 470
AfaI GTAC 1 cut(s) 337
AfiI CCNNNNNNNGG 1 cut(s) 332
AgsI TTSAA 2 cut(s) 55, 310
AjnI CCWGG 1 cut(s) 204
AluBI AGCT 1 cut(s) 116
AluI AGCT 1 cut(s) 116
Alw26I GTCTC 1 cut(s) 52
ApeKI GCWGC 3 cut(s) 113, 198, 467
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 472
AsuHPI GGTGA 2 cut(s) 315, 409
AvaII GGWCC 1 cut(s) 472
BbsI GAAGAC 1 cut(s) 348
BbvCI CCTCAGC 1 cut(s) 165
BbvI GCAGC 3 cut(s) 125, 210, 479
BccI CCATC 1 cut(s) 248
BciT130I CCWGG 1 cut(s) 206
BcoDI GTCTC 1 cut(s) 52
BfaI CTAG 3 cut(s) 117, 228, 522
BfmI CTRYAG 1 cut(s) 465
BfuAI ACCTGC 1 cut(s) 200
BisI GCNGC 3 cut(s) 114, 199, 468
BlsI GCNGC 3 cut(s) 115, 200, 469
Bme1390I CCNGG 1 cut(s) 206
Bme18I GGWCC 1 cut(s) 472
BmgT120I GGNCC 1 cut(s) 472
BmrFI CCNGG 1 cut(s) 206
BmsI GCATC 2 cut(s) 210, 271
BpiI GAAGAC 1 cut(s) 348
BpmI CTGGAG 1 cut(s) 367
Bpu10I CCTNAGC 1 cut(s) 165
BsaAI YACGTR 1 cut(s) 192
BsaBI GATNNNNATC 1 cut(s) 372
BsaI GGTCTC 1 cut(s) 52
BsaXI ACNNNNNCTCC 2 cut(s) 340, 370
Bsc4I CCNNNNNNNGG 1 cut(s) 332
Bse118I RCCGGY 1 cut(s) 394
Bse1I ACTGG 1 cut(s) 350
Bse3DI GCAATG 1 cut(s) 108
Bse8I GATNNNNATC 1 cut(s) 372
BseBI CCWGG 1 cut(s) 206
BseGI GGATG 3 cut(s) 201, 259, 286
BseJI GATNNNNATC 1 cut(s) 372
BseLI CCNNNNNNNGG 1 cut(s) 332
BseMI GCAATG 1 cut(s) 108
BseMII CTCAG 2 cut(s) 156, 227
BseNI ACTGG 1 cut(s) 350
BseXI GCAGC 3 cut(s) 125, 210, 479
BsgI GTGCAG 1 cut(s) 263
BsiSI CCGG 2 cut(s) 101, 395
BslI CCNNNNNNNGG 1 cut(s) 332
BsmAI GTCTC 1 cut(s) 52
Bso31I GGTCTC 1 cut(s) 52
Bsp143I GATC 2 cut(s) 367, 373
BspACI CCGC 2 cut(s) 293, 470
BspCNI CTCAG 2 cut(s) 157, 228
BspMAI CTGCAG 1 cut(s) 469
BspMI ACCTGC 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 52
BsrDI GCAATG 1 cut(s) 108
BsrFI RCCGGY 1 cut(s) 394
BsrI ACTGG 1 cut(s) 350
BssAI RCCGGY 1 cut(s) 394
BssMI GATC 2 cut(s) 367, 373
BssNAI GTATAC 2 cut(s) 446, 458
Bst1107I GTATAC 2 cut(s) 446, 458
Bst2UI CCWGG 1 cut(s) 206
Bst4CI ACNGT 1 cut(s) 411
BstBAI YACGTR 1 cut(s) 192
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 4 cut(s) 12, 165, 236, 452
BstENI CCTNNNNNAGG 1 cut(s) 330
BstF5I GGATG 3 cut(s) 201, 259, 286
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 2 cut(s) 370, 376
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 2 cut(s) 367, 373
BstMWI GCNNNNNNNGC 2 cut(s) 241, 527
BstNI CCWGG 1 cut(s) 206
BstNSI RCATGY 1 cut(s) 164
BstSCI CCNGG 1 cut(s) 204
BstSFI CTRYAG 1 cut(s) 465
BstV1I GCAGC 3 cut(s) 125, 210, 479
BstV2I GAAGAC 1 cut(s) 348
BstZ17I GTATAC 2 cut(s) 446, 458
BtsCI GGATG 3 cut(s) 201, 259, 286
BtsIMutI CAGTG 1 cut(s) 517
BveI ACCTGC 1 cut(s) 200
Cac8I GCNNGC 1 cut(s) 162
CfoI GCGC 1 cut(s) 198
Cfr10I RCCGGY 1 cut(s) 394
Cfr13I GGNCC 1 cut(s) 472
CpoI CGGWCCG 1 cut(s) 472
Csp6I GTAC 1 cut(s) 336
CspI CGGWCCG 1 cut(s) 472
CviAII CATG 3 cut(s) 28, 161, 285
CviJI RGCY 9 cut(s) 100, 116, 170, 227, 235, 289, 313, 521, 530
CviKI_1 RGCY 9 cut(s) 100, 116, 170, 227, 235, 289, 313, 521, 530
CviQI GTAC 1 cut(s) 336
DdeI CTNAG 4 cut(s) 12, 165, 236, 452
DpnI GATC 2 cut(s) 369, 375
DpnII GATC 2 cut(s) 367, 373
DraI TTTAAA 1 cut(s) 127
Eco31I GGTCTC 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 472
EcoNI CCTNNNNNAGG 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 204
EcoT22I ATGCAT 1 cut(s) 286
FaeI CATG 3 cut(s) 31, 164, 288
FaiI YATR 9 cut(s) 29, 162, 286, 303, 329, 446, 458, 492, 533
FatI CATG 3 cut(s) 27, 160, 284
FblI GTMKAC 2 cut(s) 445, 457
Fnu4HI GCNGC 3 cut(s) 114, 199, 468
FokI GGATG 3 cut(s) 188, 266, 293
Fsp4HI GCNGC 3 cut(s) 114, 199, 468
FspBI CTAG 3 cut(s) 117, 228, 522
GlaI GCGC 1 cut(s) 197
GluI GCNGC 3 cut(s) 114, 199, 468
GsuI CTGGAG 1 cut(s) 367
HapII CCGG 2 cut(s) 101, 395
HhaI GCGC 1 cut(s) 198
Hin1II CATG 3 cut(s) 31, 164, 288
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HinfI GANTC 2 cut(s) 104, 388
HpaII CCGG 2 cut(s) 101, 395
HphI GGTGA 2 cut(s) 315, 409
Hpy166II GTNNAC 4 cut(s) 89, 414, 446, 458
Hpy188I TCNGA 2 cut(s) 151, 378
Hpy188III TCNNGA 1 cut(s) 371
Hpy8I GTNNAC 4 cut(s) 89, 414, 446, 458
HpyAV CCTTC 4 cut(s) 14, 326, 373, 493
HpyCH4III ACNGT 1 cut(s) 411
HpyCH4IV ACGT 3 cut(s) 191, 442, 460
HpyCH4V TGCA 5 cut(s) 113, 160, 244, 284, 467
HpyF10VI GCNNNNNNNGC 2 cut(s) 241, 527
HpyF3I CTNAG 4 cut(s) 12, 165, 236, 452
HpySE526I ACGT 3 cut(s) 191, 442, 460
Hsp92II CATG 3 cut(s) 31, 164, 288
HspAI GCGC 1 cut(s) 196
Kzo9I GATC 2 cut(s) 367, 373
LpnPI CCDG 9 cut(s) 114, 156, 161, 191, 195, 218, 331, 408, 477
Lsp1109I GCAGC 3 cut(s) 125, 210, 479
LweI GCATC 2 cut(s) 210, 271
MaeI CTAG 3 cut(s) 117, 228, 522
MaeII ACGT 3 cut(s) 191, 442, 460
MaeIII GTNAC 2 cut(s) 60, 356
MalI GATC 2 cut(s) 369, 375
MboI GATC 2 cut(s) 367, 373
MboII GAAGA 1 cut(s) 353
MnlI CCTC 6 cut(s) 17, 160, 342, 397, 418, 459
Mph1103I ATGCAT 1 cut(s) 286
MseI TTAA 2 cut(s) 126, 516
MslI CAYNNNNRTG 1 cut(s) 190
MspA1I CMGCKG 1 cut(s) 470
MspI CCGG 2 cut(s) 101, 395
MspR9I CCNGG 1 cut(s) 206
MvaI CCWGG 1 cut(s) 206
MwoI GCNNNNNNNGC 2 cut(s) 241, 527
NdeII GATC 2 cut(s) 367, 373
NlaIII CATG 3 cut(s) 31, 164, 288
NmuCI GTSAC 2 cut(s) 60, 356
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 1 cut(s) 164
PaeI GCATGC 1 cut(s) 164
PfeI GAWTC 2 cut(s) 104, 388
PkrI GCNGC 3 cut(s) 115, 200, 469
Ppu21I YACGTR 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 204
PspGI CCWGG 1 cut(s) 204
PspPI GGNCC 1 cut(s) 472
PstI CTGCAG 1 cut(s) 469
RsaI GTAC 1 cut(s) 337
RsaNI GTAC 1 cut(s) 336
RseI CAYNNNNRTG 1 cut(s) 190
Rsr2I CGGWCCG 1 cut(s) 472
RsrII CGGWCCG 1 cut(s) 472
SaqAI TTAA 2 cut(s) 126, 516
SatI GCNGC 3 cut(s) 114, 199, 468
Sau3AI GATC 2 cut(s) 367, 373
Sau96I GGNCC 1 cut(s) 472
ScrFI CCNGG 1 cut(s) 206
SfaNI GCATC 2 cut(s) 210, 271
SfcI CTRYAG 1 cut(s) 465
SinI GGWCC 1 cut(s) 472
SmiMI CAYNNNNRTG 1 cut(s) 190
SphI GCATGC 1 cut(s) 164
SsiI CCGC 2 cut(s) 293, 470
SspI AATATT 1 cut(s) 137
SspMI CTAG 3 cut(s) 117, 228, 522
StyD4I CCNGG 1 cut(s) 204
TaaI ACNGT 1 cut(s) 411
TaiI ACGT 3 cut(s) 194, 445, 463
TaqII GACCGA 1 cut(s) 489
TfiI GAWTC 2 cut(s) 104, 388
Tru1I TTAA 2 cut(s) 126, 516
Tru9I TTAA 2 cut(s) 126, 516
TscAI CASTG 1 cut(s) 517
TseFI GTSAC 2 cut(s) 60, 356
TseI GCWGC 3 cut(s) 113, 198, 467
Tsp45I GTSAC 2 cut(s) 60, 356
TspDTI ATGAA 1 cut(s) 174
TspRI CASTG 1 cut(s) 517
VpaK11BI GGWCC 1 cut(s) 472
XagI CCTNNNNNAGG 1 cut(s) 330
XceI RCATGY 1 cut(s) 164
XmiI GTMKAC 2 cut(s) 445, 457
XspI CTAG 3 cut(s) 117, 228, 522
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.