FvH4_5g23720

Isoflavone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001108
Physical Location & Seq
Reverse (-)
15054976 .. 15055810
835 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g23720.t1

Sequence Viewer

Length: 567 bp
ATGGGACATGCACTTGAACCAGGAAGAGTGACATTTGATCAGAAAATGGCGGTGAGGAAGGCAATAGAAGACGCTAAAATACCCTTCACTTATGTGTGTGGTACTTGCTTCGCCGGTTATTTTGCTGGGAACCTCTCGCAGATGGGAACACTTGTTCCTCCAAAGGATGAAGTTCTTATTTATGGAGATGGAAACACTAAAGTATCTATAATGGATGAAGATGACGTCGCAAGCTATACAATCAAGACGATTGATGATCCGCGAACTTTGAACAAAACATTGTACCTTCTTCCCCCGGAAAACCTACTCACTCAAAGACAATTGGAGATTTGGGAAAATCTCATTGGAAAGAAACTAGAGAAAAATTCTCTTTCGAAAGAAGATCTCCTTGCCTCTATGACAGGTATGGAATATGCAGGCCAGGTCGGAGCGGGGCATTTCTGTCACATTTTCTATGAAGGCTCTTTGACAAACTTCGAAATAGGGGAGGAAGGAGAAGAAGCTTCAAAGCTTTATCCAAAAGTGAAATATACCCGCATCGATGAATACTTGAAGATTTATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

189

Amino Acids

21.09

Weight (kDa)

4.96

Isoelectric Point (pI)

28.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 184 1.9e-29 NmrA-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 228
AccBSI CCGCTC 1 cut(s) 431
AccII CGCG 1 cut(s) 262
AciI CCGC 4 cut(s) 50, 260, 431, 535
AclWI GGATC 1 cut(s) 251
AcsI RAATTY 1 cut(s) 364
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 2 cut(s) 103, 284
AgsI TTSAA 4 cut(s) 17, 271, 507, 553
AjnI CCWGG 2 cut(s) 19, 420
AjuI GAANNNNNNNTTGG 2 cut(s) 327, 359
AleI CACNNNNGTG 1 cut(s) 92
AloI GAACNNNNNNTCC 4 cut(s) 138, 139, 170, 171
AluBI AGCT 3 cut(s) 234, 503, 511
AluI AGCT 3 cut(s) 234, 503, 511
AlwI GGATC 1 cut(s) 251
AoxI GGCC 1 cut(s) 418
ApoI RAATTY 1 cut(s) 364
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 1 cut(s) 64
AsuII TTCGAA 2 cut(s) 374, 477
BaeI ACNNNNGTAYC 2 cut(s) 186, 219
BbsI GAAGAC 1 cut(s) 75
BccI CCATC 2 cut(s) 136, 182
BciT130I CCWGG 2 cut(s) 21, 422
BclI TGATCA 1 cut(s) 37
BcnI CCSGG 1 cut(s) 296
BfaI CTAG 1 cut(s) 356
BglII AGATCT 1 cut(s) 382
Bme1390I CCNGG 3 cut(s) 21, 296, 422
BmiI GGNNCC 1 cut(s) 131
BmrFI CCNGG 3 cut(s) 21, 296, 422
BmsI GCATC 1 cut(s) 546
BpiI GAAGAC 1 cut(s) 75
Bpu14I TTCGAA 2 cut(s) 374, 477
BpuMI CCSGG 1 cut(s) 296
Bsa29I ATCGAT 1 cut(s) 540
BsaHI GRCGYC 1 cut(s) 225
BsaJI CCNNGG 1 cut(s) 294
Bse118I RCCGGY 1 cut(s) 113
BseBI CCWGG 2 cut(s) 21, 422
BseCI ATCGAT 1 cut(s) 540
BseDI CCNNGG 1 cut(s) 294
BseGI GGATG 2 cut(s) 172, 220
BseYI CCCAGC 1 cut(s) 125
Bsh1236I CGCG 1 cut(s) 262
BshFI GGCC 1 cut(s) 420
BshVI ATCGAT 1 cut(s) 540
BsiSI CCGG 2 cut(s) 114, 296
BslFI GGGAC 1 cut(s) 18
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 1 cut(s) 420
Bsp119I TTCGAA 2 cut(s) 374, 477
Bsp143I GATC 3 cut(s) 37, 256, 382
BspACI CCGC 4 cut(s) 50, 260, 431, 535
BspANI GGCC 1 cut(s) 420
BspDI ATCGAT 1 cut(s) 540
BspFNI CGCG 1 cut(s) 262
BspLI GGNNCC 1 cut(s) 131
BspPI GGATC 1 cut(s) 251
BspT104I TTCGAA 2 cut(s) 374, 477
BsrBI CCGCTC 1 cut(s) 431
BsrFI RCCGGY 1 cut(s) 113
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 1 cut(s) 294
BssMI GATC 3 cut(s) 37, 256, 382
BssNI GRCGYC 1 cut(s) 225
Bst2UI CCWGG 2 cut(s) 21, 422
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 225
BstBI TTCGAA 2 cut(s) 374, 477
BstC8I GCNNGC 2 cut(s) 232, 418
BstF5I GGATG 2 cut(s) 172, 220
BstFNI CGCG 1 cut(s) 262
BstKTI GATC 3 cut(s) 40, 259, 385
BstMBI GATC 3 cut(s) 37, 256, 382
BstNI CCWGG 2 cut(s) 21, 422
BstNSI RCATGY 1 cut(s) 11
BstSCI CCNGG 3 cut(s) 19, 294, 420
BstUI CGCG 1 cut(s) 262
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 382
BstYI RGATCY 1 cut(s) 382
Bsu15I ATCGAT 1 cut(s) 540
BsuRI GGCC 1 cut(s) 420
BsuTUI ATCGAT 1 cut(s) 540
BtsCI GGATG 2 cut(s) 172, 220
Cac8I GCNNGC 2 cut(s) 232, 418
Cfr10I RCCGGY 1 cut(s) 113
ClaI ATCGAT 1 cut(s) 540
CseI GACGC 1 cut(s) 80
Csp6I GTAC 2 cut(s) 102, 283
CviAII CATG 2 cut(s) 8, 564
CviJI RGCY 5 cut(s) 234, 420, 462, 503, 511
CviKI_1 RGCY 5 cut(s) 234, 420, 462, 503, 511
CviQI GTAC 2 cut(s) 102, 283
DpnI GATC 3 cut(s) 39, 258, 384
DpnII GATC 3 cut(s) 37, 256, 382
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
EcoRII CCWGG 2 cut(s) 19, 420
EcoT22I ATGCAT 1 cut(s) 565
FaeI CATG 2 cut(s) 11, 567
FalI AAGNNNNNCTT 2 cut(s) 372, 404
FaqI GGGAC 1 cut(s) 18
FatI CATG 2 cut(s) 7, 563
FauI CCCGC 2 cut(s) 424, 542
FbaI TGATCA 1 cut(s) 37
FokI GGATG 2 cut(s) 179, 227
FspBI CTAG 1 cut(s) 356
GsaI CCCAGC 1 cut(s) 129
HaeIII GGCC 1 cut(s) 420
HapII CCGG 2 cut(s) 114, 296
HgaI GACGC 1 cut(s) 80
Hin1I GRCGYC 1 cut(s) 225
Hin1II CATG 2 cut(s) 11, 567
HindIII AAGCTT 2 cut(s) 501, 509
HpaII CCGG 2 cut(s) 114, 296
HphI GGTGA 1 cut(s) 64
Hpy188I TCNGA 2 cut(s) 42, 428
Hpy188III TCNNGA 1 cut(s) 244
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 5 cut(s) 52, 94, 296, 452, 485
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 3 cut(s) 11, 416, 563
HpySE526I ACGT 1 cut(s) 225
Hsp92I GRCGYC 1 cut(s) 225
Hsp92II CATG 2 cut(s) 11, 567
Ksp22I TGATCA 1 cut(s) 37
Kzo9I GATC 3 cut(s) 37, 256, 382
LmnI GCTCC 1 cut(s) 428
LpnPI CCDG 9 cut(s) 6, 33, 111, 127, 309, 387, 402, 407, 434
LweI GCATC 1 cut(s) 546
MaeI CTAG 1 cut(s) 356
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 2 cut(s) 28, 443
MalI GATC 3 cut(s) 39, 258, 384
MbiI CCGCTC 1 cut(s) 431
MboI GATC 3 cut(s) 37, 256, 382
MboII GAAGA 7 cut(s) 36, 80, 230, 281, 392, 509, 565
MfeI CAATTG 1 cut(s) 320
MflI RGATCY 1 cut(s) 382
MluCI AATT 2 cut(s) 320, 364
MmeI TCCRAC 1 cut(s) 406
MnlI CCTC 5 cut(s) 48, 143, 168, 403, 481
Mph1103I ATGCAT 1 cut(s) 565
MslI CAYNNNNRTG 1 cut(s) 92
MspI CCGG 2 cut(s) 114, 296
MspR9I CCNGG 3 cut(s) 21, 296, 422
MunI CAATTG 1 cut(s) 320
MvaI CCWGG 2 cut(s) 21, 422
MvnI CGCG 1 cut(s) 262
NciI CCSGG 1 cut(s) 296
NdeII GATC 3 cut(s) 37, 256, 382
NlaIII CATG 2 cut(s) 11, 567
NlaIV GGNNCC 1 cut(s) 131
NmuCI GTSAC 2 cut(s) 28, 443
NsiI ATGCAT 1 cut(s) 565
NspI RCATGY 1 cut(s) 11
NspV TTCGAA 2 cut(s) 374, 477
OliI CACNNNNGTG 1 cut(s) 92
Psp6I CCWGG 2 cut(s) 19, 420
PspFI CCCAGC 1 cut(s) 125
PspGI CCWGG 2 cut(s) 19, 420
PspN4I GGNNCC 1 cut(s) 131
PsuI RGATCY 1 cut(s) 382
RsaI GTAC 2 cut(s) 103, 284
RsaNI GTAC 2 cut(s) 102, 283
RseI CAYNNNNRTG 1 cut(s) 92
Sau3AI GATC 3 cut(s) 37, 256, 382
ScrFI CCNGG 3 cut(s) 21, 296, 422
SetI ASST 9 cut(s) 135, 228, 236, 288, 306, 406, 426, 505, 513
SfaNI GCATC 1 cut(s) 546
SfuI TTCGAA 2 cut(s) 374, 477
SmiMI CAYNNNNRTG 1 cut(s) 92
Sse9I AATT 2 cut(s) 320, 364
SsiI CCGC 4 cut(s) 50, 260, 431, 535
SspMI CTAG 1 cut(s) 356
StyD4I CCNGG 3 cut(s) 19, 294, 420
TaiI ACGT 1 cut(s) 228
TaqI TCGA 3 cut(s) 374, 477, 540
TasI AATT 2 cut(s) 320, 364
TseFI GTSAC 2 cut(s) 28, 443
Tsp45I GTSAC 2 cut(s) 28, 443
TspDTI ATGAA 4 cut(s) 183, 231, 471, 558
XapI RAATTY 1 cut(s) 364
XceI RCATGY 1 cut(s) 11
XspI CTAG 1 cut(s) 356
ZraI GACGTC 1 cut(s) 226
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.