RchiOBHm_Chr7g0213011

Isoflavone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001108
Physical Location & Seq
Reverse (-)
30188611 .. 30189301
691 bp
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UTR
Exon/CDS
Intron
PRQ19065

Sequence Viewer

Length: 402 bp
ATGTATGGAGATGGCAACCGCAAAGTGATACTTCTGGATGAGGATGACATTGCAACATACGCAATAAAAACGATATTTGATGATCCCCGAACATTAAACAAAACATTGCACCTTCGTCCGCCCGAGTGCATAGTCTCTCAAAGACAGTTGGTGGAGATCGATCTGGGAAGGCCTTATTGGAAAGAAACTAGAAAAGGTTACCCTTTCCAAAGAAGACTTCCTTGCCTCTATGAAAGATATGGACACTATGCAAATCAGGTTGGAGCAGGTCATTTCCATGACATGTACTATGAAGGTTGTTTGACAAACTCTGAAATAGGGGAAAATGCCGAAGAAGCTTCAAAGCTTTATCCCGAAGTGAAGTACACTCGCATGGATGAATTCTTGAAAATTTATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

133

Amino Acids

15.7

Weight (kDa)

5.81

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 129 7.9e-06 NmrA-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 257
AciI CCGC 2 cut(s) 19, 119
AclWI GGATC 1 cut(s) 77
AcsI RAATTY 2 cut(s) 380, 390
AfaI GTAC 2 cut(s) 287, 365
AflIII ACRYGT 1 cut(s) 282
AgsI TTSAA 2 cut(s) 342, 388
AjuI GAANNNNNNNTTGG 4 cut(s) 160, 192, 201, 233
AluBI AGCT 2 cut(s) 338, 346
AluI AGCT 2 cut(s) 338, 346
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 1 cut(s) 77
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 1 cut(s) 170
ApoI RAATTY 2 cut(s) 380, 390
AvaI CYCGRG 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 220
BccI CCATC 1 cut(s) 5
BcoDI GTCTC 1 cut(s) 139
BfaI CTAG 1 cut(s) 189
BfuAI ACCTGC 1 cut(s) 257
BmeT110I CYCGRG 1 cut(s) 122
BpiI GAAGAC 1 cut(s) 220
Bsa29I ATCGAT 1 cut(s) 159
Bse3DI GCAATG 2 cut(s) 48, 104
BseCI ATCGAT 1 cut(s) 159
BseGI GGATG 3 cut(s) 43, 49, 382
BseMI GCAATG 2 cut(s) 48, 104
BshFI GGCC 1 cut(s) 172
BshVI ATCGAT 1 cut(s) 159
BsiHKCI CYCGRG 1 cut(s) 122
BsmAI GTCTC 1 cut(s) 139
BsnI GGCC 1 cut(s) 172
BsoBI CYCGRG 1 cut(s) 122
Bsp143I GATC 3 cut(s) 82, 156, 160
BspACI CCGC 2 cut(s) 19, 119
BspANI GGCC 1 cut(s) 172
BspDI ATCGAT 1 cut(s) 159
BspMI ACCTGC 1 cut(s) 257
BspPI GGATC 1 cut(s) 77
BsrDI GCAATG 2 cut(s) 48, 104
BssMI GATC 3 cut(s) 82, 156, 160
Bst4CI ACNGT 1 cut(s) 147
BstEII GGTNACC 1 cut(s) 197
BstF5I GGATG 3 cut(s) 43, 49, 382
BstKTI GATC 3 cut(s) 85, 159, 163
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 3 cut(s) 82, 156, 160
BstMWI GCNNNNNNNGC 2 cut(s) 59, 335
BstNSI RCATGY 1 cut(s) 286
BstPI GGTNACC 1 cut(s) 197
BstV2I GAAGAC 1 cut(s) 220
Bsu15I ATCGAT 1 cut(s) 159
BsuRI GGCC 1 cut(s) 172
BsuTUI ATCGAT 1 cut(s) 159
BtsCI GGATG 3 cut(s) 43, 49, 382
BveI ACCTGC 1 cut(s) 257
ClaI ATCGAT 1 cut(s) 159
Csp6I GTAC 2 cut(s) 286, 364
CviAII CATG 3 cut(s) 278, 283, 373
CviJI RGCY 3 cut(s) 172, 338, 346
CviKI_1 RGCY 3 cut(s) 172, 338, 346
CviQI GTAC 2 cut(s) 286, 364
DpnI GATC 3 cut(s) 84, 158, 162
DpnII GATC 3 cut(s) 82, 156, 160
EciI GGCGGA 1 cut(s) 108
Eco147I AGGCCT 1 cut(s) 172
Eco88I CYCGRG 1 cut(s) 122
Eco91I GGTNACC 1 cut(s) 197
EcoO65I GGTNACC 1 cut(s) 197
EcoRI GAATTC 1 cut(s) 380
FaeI CATG 3 cut(s) 281, 286, 376
FalI AAGNNNNNCTT 4 cut(s) 15, 47, 205, 237
FatI CATG 3 cut(s) 277, 282, 372
FokI GGATG 3 cut(s) 50, 56, 389
FspBI CTAG 1 cut(s) 189
HaeIII GGCC 1 cut(s) 172
Hin1II CATG 3 cut(s) 281, 286, 376
HindIII AAGCTT 2 cut(s) 336, 344
Hpy166II GTNNAC 1 cut(s) 366
Hpy188I TCNGA 1 cut(s) 313
Hpy188III TCNNGA 3 cut(s) 35, 353, 385
Hpy8I GTNNAC 1 cut(s) 366
HpyAV CCTTC 3 cut(s) 122, 162, 287
HpyCH4III ACNGT 1 cut(s) 147
HpyCH4V TGCA 4 cut(s) 53, 109, 129, 251
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 335
Hsp92II CATG 3 cut(s) 281, 286, 376
Kzo9I GATC 3 cut(s) 82, 156, 160
LmnI GCTCC 1 cut(s) 263
LpnPI CCDG 4 cut(s) 20, 149, 242, 252
MaeI CTAG 1 cut(s) 189
MaeIII GTNAC 1 cut(s) 197
MalI GATC 3 cut(s) 84, 158, 162
MboI GATC 3 cut(s) 82, 156, 160
MboII GAAGA 2 cut(s) 225, 344
MluCI AATT 2 cut(s) 380, 390
MmeI TCCRAC 1 cut(s) 241
MnlI CCTC 2 cut(s) 34, 236
MseI TTAA 2 cut(s) 95, 400
MslI CAYNNNNRTG 2 cut(s) 276, 371
MwoI GCNNNNNNNGC 2 cut(s) 59, 335
NdeII GATC 3 cut(s) 82, 156, 160
NlaIII CATG 3 cut(s) 281, 286, 376
NspI RCATGY 1 cut(s) 286
PceI AGGCCT 1 cut(s) 172
PciI ACATGT 1 cut(s) 282
PscI ACATGT 1 cut(s) 282
PspEI GGTNACC 1 cut(s) 197
RsaI GTAC 2 cut(s) 287, 365
RsaNI GTAC 2 cut(s) 286, 364
RseI CAYNNNNRTG 2 cut(s) 276, 371
SaqAI TTAA 2 cut(s) 95, 400
Sau3AI GATC 3 cut(s) 82, 156, 160
SetI ASST 7 cut(s) 114, 199, 261, 271, 298, 340, 348
SmiMI CAYNNNNRTG 2 cut(s) 276, 371
Sse9I AATT 2 cut(s) 380, 390
SseBI AGGCCT 1 cut(s) 172
SsiI CCGC 2 cut(s) 19, 119
SspMI CTAG 1 cut(s) 189
StuI AGGCCT 1 cut(s) 172
TaaI ACNGT 1 cut(s) 147
TaqI TCGA 1 cut(s) 159
TasI AATT 2 cut(s) 380, 390
TatI WGTACW 2 cut(s) 285, 363
Tru1I TTAA 2 cut(s) 95, 400
Tru9I TTAA 2 cut(s) 95, 400
TspDTI ATGAA 3 cut(s) 246, 306, 393
XapI RAATTY 2 cut(s) 380, 390
XceI RCATGY 1 cut(s) 286
XspI CTAG 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.