Rw7G023480

Isoflavone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001108
Physical Location & Seq
Reverse (-)
28191052 .. 28207457
16406 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G023480.1

Sequence Viewer

Length: 837 bp
ATGGGGAGAAGGATTGTCAAGGCAAGACTAGCAGCAGACCACCAGACTTATGTTCTGCAAAGGCCGGCGCCAGAGATCGGCCTCGACATTGAGAAGCTACAAATGCTCTTCTCGTTTACGAAGCAAGGGGCTCACCTCGTCGAGGGTTCGTTTTCGGATTTCCAGAGCCTCGTCGACGCTGTCAAGCTAGTTGATGTTGTCATATGCACCATGTCGGGGACACATATCCGGAGTCACAACATTATGTTGCAGCTCAAGCTCGTTGAAGCCATTAAAGAAGCTGGAAATAGTTTGTCATGGACGCGCGTGGCACGTATGGGAAATGCTCTTGAACCAGGAAGAGTTCCGTTTGATGACAAAATGAAAGTGAGAAAAGCAATAGAAGATGCTAACATCCCCTTCACATATGTATGTGGTATTGGCATCGCGGCTTATCATGCCGGCAACCTCTGCCAAATGGGAACACGTACTGTGATACTTCTGGATGAGGATGACATTGCAACATACGCAATAAAAACGATTGATGATCCCCGAACATTAAACAAAACATTGCACCTTCGTCCGCCCGAGTGCATAGTCTCTCAAAGACAGTTGAAACTAGAAAAGGTTACCCTTTCCAAAGAAGACTTCCTTGCCTCTATGAAAGTACGTAATACATTTATATATTTCAGATATGGACACTATGTAAATCAGGTTGGAGCAGGTCATTTCCATGACATGTACTATGAAGGTTGTTTGACAAACTCTGAAATAGGGGAAAATGCCGAAGAAGCTTCAAAGCTTTATCCCGAAGTGAAGTACACTCGCATGGATGAATTCTTGAAAATTTATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

278

Amino Acids

31.5

Weight (kDa)

7.11

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 2 - 274 3.3e-26 NmrA-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 692
AccB1I GGYRCC 1 cut(s) 67
AccI GTMKAC 1 cut(s) 174
AccII CGCG 3 cut(s) 304, 306, 428
AccIII TCCGGA 1 cut(s) 228
AciI CCGC 2 cut(s) 428, 563
AclWI GGATC 1 cut(s) 521
AcsI RAATTY 2 cut(s) 815, 825
AcyI GRCGYC 1 cut(s) 68
AfaI GTAC 4 cut(s) 469, 648, 722, 800
AfiI CCNNNNNNNGG 3 cut(s) 77, 142, 216
AflIII ACRYGT 2 cut(s) 464, 717
AgsI TTSAA 5 cut(s) 266, 332, 595, 777, 823
AjnI CCWGG 1 cut(s) 334
AjuI GAANNNNNNNTTGG 2 cut(s) 611, 643
AluBI AGCT 7 cut(s) 97, 187, 253, 259, 281, 773, 781
AluI AGCT 7 cut(s) 97, 187, 253, 259, 281, 773, 781
Alw26I GTCTC 1 cut(s) 583
AlwI GGATC 1 cut(s) 521
Ama87I CYCGRG 1 cut(s) 566
Aor13HI TCCGGA 1 cut(s) 228
AoxI GGCC 2 cut(s) 62, 79
ApeKI GCWGC 2 cut(s) 32, 250
ApoI RAATTY 2 cut(s) 815, 825
AspLEI GCGC 2 cut(s) 70, 306
AsuHPI GGTGA 1 cut(s) 125
AvaI CYCGRG 1 cut(s) 566
BanI GGYRCC 1 cut(s) 67
BanII GRGCYC 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 630
BbvI GCAGC 2 cut(s) 44, 262
BciT130I CCWGG 1 cut(s) 336
BcoDI GTCTC 1 cut(s) 583
BfaI CTAG 3 cut(s) 29, 188, 599
BfoI RGCGCY 1 cut(s) 71
BfuAI ACCTGC 1 cut(s) 692
BisI GCNGC 3 cut(s) 33, 251, 429
BlsI GCNGC 3 cut(s) 34, 252, 430
Bme1390I CCNGG 1 cut(s) 336
BmeT110I CYCGRG 1 cut(s) 566
BmiI GGNNCC 1 cut(s) 69
BmrFI CCNGG 1 cut(s) 336
BmsI GCATC 2 cut(s) 376, 432
BpiI GAAGAC 1 cut(s) 630
BpuEI CTTGAG 1 cut(s) 239
BsaAI YACGTR 3 cut(s) 314, 467, 650
BsaHI GRCGYC 1 cut(s) 68
BsaWI WCCGGW 1 cut(s) 228
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 142, 216
Bse118I RCCGGY 2 cut(s) 64, 440
Bse3DI GCAATG 2 cut(s) 495, 548
BseAI TCCGGA 1 cut(s) 228
BseBI CCWGG 1 cut(s) 336
BseGI GGATG 4 cut(s) 393, 490, 496, 817
BseLI CCNNNNNNNGG 3 cut(s) 77, 142, 216
BseMI GCAATG 2 cut(s) 495, 548
BseXI GCAGC 2 cut(s) 44, 262
Bsh1236I CGCG 3 cut(s) 304, 306, 428
BshFI GGCC 2 cut(s) 64, 81
BshNI GGYRCC 1 cut(s) 67
BsiHKCI CYCGRG 1 cut(s) 566
BsiSI CCGG 3 cut(s) 65, 229, 441
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 3 cut(s) 77, 142, 216
BsmAI GTCTC 1 cut(s) 583
BsmFI GGGAC 1 cut(s) 232
BsnI GGCC 2 cut(s) 64, 81
BsoBI CYCGRG 1 cut(s) 566
Bsp1286I GDGCHC 1 cut(s) 133
Bsp13I TCCGGA 1 cut(s) 228
Bsp143I GATC 2 cut(s) 75, 526
BspACI CCGC 2 cut(s) 428, 563
BspANI GGCC 2 cut(s) 64, 81
BspEI TCCGGA 1 cut(s) 228
BspFNI CGCG 3 cut(s) 304, 306, 428
BspLI GGNNCC 1 cut(s) 69
BspMI ACCTGC 1 cut(s) 692
BspPI GGATC 1 cut(s) 521
BspQI GCTCTTC 1 cut(s) 113
BspT107I GGYRCC 1 cut(s) 67
BsrDI GCAATG 2 cut(s) 495, 548
BsrFI RCCGGY 2 cut(s) 64, 440
BssAI RCCGGY 2 cut(s) 64, 440
BssMI GATC 2 cut(s) 75, 526
BssNI GRCGYC 1 cut(s) 68
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 2 cut(s) 472, 591
Bst6I CTCTTC 2 cut(s) 113, 334
BstACI GRCGYC 1 cut(s) 68
BstAPI GCANNNNNTGC 1 cut(s) 450
BstBAI YACGTR 3 cut(s) 314, 467, 650
BstC8I GCNNGC 2 cut(s) 66, 442
BstEII GGTNACC 1 cut(s) 607
BstENI CCTNNNNNAGG 1 cut(s) 140
BstF5I GGATG 4 cut(s) 393, 490, 496, 817
BstFNI CGCG 3 cut(s) 304, 306, 428
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 2 cut(s) 70, 306
BstKTI GATC 2 cut(s) 78, 529
BstMAI GTCTC 1 cut(s) 583
BstMBI GATC 2 cut(s) 75, 526
BstMWI GCNNNNNNNGC 7 cut(s) 29, 103, 256, 437, 450, 506, 770
BstNI CCWGG 1 cut(s) 336
BstNSI RCATGY 1 cut(s) 721
BstPI GGTNACC 1 cut(s) 607
BstSCI CCNGG 1 cut(s) 334
BstSNI TACGTA 1 cut(s) 650
BstUI CGCG 3 cut(s) 304, 306, 428
BstV1I GCAGC 2 cut(s) 44, 262
BstV2I GAAGAC 1 cut(s) 630
BsuRI GGCC 2 cut(s) 64, 81
BtgZI GCGATG 1 cut(s) 409
BtsCI GGATG 4 cut(s) 393, 490, 496, 817
BveI ACCTGC 1 cut(s) 692
Cac8I GCNNGC 2 cut(s) 66, 442
CfoI GCGC 2 cut(s) 70, 306
Cfr10I RCCGGY 2 cut(s) 64, 440
CseI GACGC 2 cut(s) 185, 310
Csp6I GTAC 4 cut(s) 468, 647, 721, 799
CviAII CATG 6 cut(s) 211, 297, 437, 713, 718, 808
CviQI GTAC 4 cut(s) 468, 647, 721, 799
DinI GGCGCC 1 cut(s) 69
DpnI GATC 2 cut(s) 77, 528
DpnII GATC 2 cut(s) 75, 526
Eam1104I CTCTTC 2 cut(s) 113, 334
EarI CTCTTC 2 cut(s) 113, 334
EciI GGCGGA 1 cut(s) 552
Eco105I TACGTA 1 cut(s) 650
Eco24I GRGCYC 1 cut(s) 133
Eco88I CYCGRG 1 cut(s) 566
Eco91I GGTNACC 1 cut(s) 607
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoO65I GGTNACC 1 cut(s) 607
EcoRI GAATTC 1 cut(s) 815
EcoRII CCWGG 1 cut(s) 334
EcoT38I GRGCYC 1 cut(s) 133
EgeI GGCGCC 1 cut(s) 69
EheI GGCGCC 1 cut(s) 69
FaeI CATG 6 cut(s) 214, 300, 440, 716, 721, 811
FalI AAGNNNNNCTT 2 cut(s) 615, 647
FaqI GGGAC 1 cut(s) 232
FatI CATG 6 cut(s) 210, 296, 436, 712, 717, 807
FauNDI CATATG 2 cut(s) 203, 406
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 3 cut(s) 33, 251, 429
FokI GGATG 4 cut(s) 380, 497, 503, 824
FriOI GRGCYC 1 cut(s) 133
Fsp4HI GCNGC 3 cut(s) 33, 251, 429
FspBI CTAG 3 cut(s) 29, 188, 599
GlaI GCGC 2 cut(s) 69, 305
GluI GCNGC 3 cut(s) 33, 251, 429
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 2 cut(s) 64, 81
HapII CCGG 3 cut(s) 65, 229, 441
HgaI GACGC 2 cut(s) 185, 310
HhaI GCGC 2 cut(s) 70, 306
Hin1I GRCGYC 1 cut(s) 68
Hin1II CATG 6 cut(s) 214, 300, 440, 716, 721, 811
Hin6I GCGC 2 cut(s) 68, 304
HinP1I GCGC 2 cut(s) 68, 304
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HindIII AAGCTT 2 cut(s) 771, 779
HinfI GANTC 1 cut(s) 232
HpaII CCGG 3 cut(s) 65, 229, 441
HphI GGTGA 1 cut(s) 125
Hpy166II GTNNAC 3 cut(s) 117, 175, 801
Hpy188I TCNGA 3 cut(s) 157, 671, 748
Hpy188III TCNNGA 6 cut(s) 163, 229, 329, 482, 788, 820
Hpy8I GTNNAC 3 cut(s) 117, 175, 801
Hpy99I CGWCG 3 cut(s) 143, 176, 179
HpyAV CCTTC 4 cut(s) 3, 409, 566, 722
HpyCH4III ACNGT 2 cut(s) 472, 591
HpyCH4IV ACGT 3 cut(s) 313, 466, 649
HpyCH4V TGCA 6 cut(s) 58, 207, 250, 500, 553, 573
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 103, 256, 437, 450, 506, 770
HpySE526I ACGT 3 cut(s) 313, 466, 649
Hsp92I GRCGYC 1 cut(s) 68
Hsp92II CATG 6 cut(s) 214, 300, 440, 716, 721, 811
HspAI GCGC 2 cut(s) 68, 304
KasI GGCGCC 1 cut(s) 67
Kpn2I TCCGGA 1 cut(s) 228
KroI GCCGGC 2 cut(s) 64, 440
KroNI GCCGGC 2 cut(s) 66, 442
Kzo9I GATC 2 cut(s) 75, 526
LguI GCTCTTC 1 cut(s) 113
LmnI GCTCC 1 cut(s) 698
Lsp1109I GCAGC 2 cut(s) 44, 262
LweI GCATC 2 cut(s) 376, 432
MaeI CTAG 3 cut(s) 29, 188, 599
MaeII ACGT 3 cut(s) 313, 466, 649
MaeIII GTNAC 2 cut(s) 233, 607
MalI GATC 2 cut(s) 77, 528
MboI GATC 2 cut(s) 75, 526
MboII GAAGA 5 cut(s) 100, 351, 395, 635, 779
MhlI GDGCHC 1 cut(s) 133
MluCI AATT 2 cut(s) 815, 825
Mly113I GGCGCC 1 cut(s) 68
MlyI GAGTC 1 cut(s) 241
MmeI TCCRAC 1 cut(s) 676
MnlI CCTC 7 cut(s) 92, 136, 146, 179, 458, 481, 646
MroI TCCGGA 1 cut(s) 228
MroNI GCCGGC 2 cut(s) 64, 440
MseI TTAA 3 cut(s) 273, 539, 835
MslI CAYNNNNRTG 3 cut(s) 409, 711, 806
MspI CCGG 3 cut(s) 65, 229, 441
MspR9I CCNGG 1 cut(s) 336
MvaI CCWGG 1 cut(s) 336
MvnI CGCG 3 cut(s) 304, 306, 428
MwoI GCNNNNNNNGC 7 cut(s) 29, 103, 256, 437, 450, 506, 770
NaeI GCCGGC 2 cut(s) 66, 442
NarI GGCGCC 1 cut(s) 68
NdeI CATATG 2 cut(s) 203, 406
NdeII GATC 2 cut(s) 75, 526
NgoMIV GCCGGC 2 cut(s) 64, 440
NlaIII CATG 6 cut(s) 214, 300, 440, 716, 721, 811
NlaIV GGNNCC 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 233
NspI RCATGY 1 cut(s) 721
PciI ACATGT 1 cut(s) 717
PciSI GCTCTTC 1 cut(s) 113
PdiI GCCGGC 2 cut(s) 66, 442
PflFI GACNNNGTC 1 cut(s) 179
PkrI GCNGC 3 cut(s) 34, 252, 430
PleI GAGTC 1 cut(s) 240
PluTI GGCGCC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 240
Ppu21I YACGTR 3 cut(s) 314, 467, 650
PscI ACATGT 1 cut(s) 717
Psp6I CCWGG 1 cut(s) 334
PspEI GGTNACC 1 cut(s) 607
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 1 cut(s) 69
PsyI GACNNNGTC 1 cut(s) 179
RsaI GTAC 4 cut(s) 469, 648, 722, 800
RsaNI GTAC 4 cut(s) 468, 647, 721, 799
RseI CAYNNNNRTG 3 cut(s) 409, 711, 806
SalI GTCGAC 1 cut(s) 173
SapI GCTCTTC 1 cut(s) 113
SaqAI TTAA 3 cut(s) 273, 539, 835
SatI GCNGC 3 cut(s) 33, 251, 429
Sau3AI GATC 2 cut(s) 75, 526
SchI GAGTC 1 cut(s) 241
ScrFI CCNGG 1 cut(s) 336
SduI GDGCHC 1 cut(s) 133
SfaNI GCATC 2 cut(s) 376, 432
SfoI GGCGCC 1 cut(s) 69
SgrDI CGTCGACG 1 cut(s) 173
SmiMI CAYNNNNRTG 3 cut(s) 409, 711, 806
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
SnaBI TACGTA 1 cut(s) 650
Sse9I AATT 2 cut(s) 815, 825
SsiI CCGC 2 cut(s) 428, 563
SspDI GGCGCC 1 cut(s) 67
SspMI CTAG 3 cut(s) 29, 188, 599
StyD4I CCNGG 1 cut(s) 334
TaaI ACNGT 2 cut(s) 472, 591
TaiI ACGT 3 cut(s) 316, 469, 652
TaqI TCGA 3 cut(s) 84, 141, 174
TasI AATT 2 cut(s) 815, 825
TatI WGTACW 2 cut(s) 720, 798
TauI GCSGC 1 cut(s) 431
Tru1I TTAA 3 cut(s) 273, 539, 835
Tru9I TTAA 3 cut(s) 273, 539, 835
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 2 cut(s) 32, 250
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 4 cut(s) 377, 656, 741, 828
TspGWI ACGGA 1 cut(s) 336
Tth111I GACNNNGTC 1 cut(s) 179
XagI CCTNNNNNAGG 1 cut(s) 140
XapI RAATTY 2 cut(s) 815, 825
XceI RCATGY 1 cut(s) 721
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 3 cut(s) 29, 188, 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.