Rw7G023450

Isoflavone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001108
Physical Location & Seq
Reverse (-)
28105060 .. 28107319
2260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G023450.1

Sequence Viewer

Length: 951 bp
ATGGCAACTACAAAGAGCAAGGTTCTTGTTGTTGGTGGAACTGGTTACATGGGGAGAAGGATTGTCAAGGCAAGCCTAGCAGCAGGCCACCAGACTTATGTTCTGCAAAGGCCGGCGCCAGAGATCGGCCTCGACATTGAGAAGCTACAAATGCTCTTCTCGTTTAAGAAGCAAGGGGCTCACCTCGTCGAGGGTTCGTTTTCGGATTTCCAGAGCCTCGTCGACGCTGTCAAGCTAGTTGATGTTGTCATATGCACCATGTCGGGAACACATATCCGGAGTCACAACATTATGTTGCAGCTCAAGCTTGTTGAAGCCATTAAAGAAGCTGGAAATGTTAAGCGTTTCTTGCCATCAGAGTATGGCATGGACGTGGCACGTATGGGAAATGCTCTTGAACCAGGAAGAGTTCCGTTTGATGATAAAATGAAAATTAGAAAAGCAATAGAAGATGCTAACATCCCCTTCACATATGTATGTGGTATTGGCTTCGCGGCTTATCATGCCGGCAACCTCTGCCAAATGGGAACACTGGTTCCTCCAAGGGATAAGGTGCTTATGTATGGAGATGGCAACCGCAAAGTGATACTTCTGGATGAGGATGACATTGCAACATACGCAATAAAAACGATTGATGATCCCCGAACATTAAACAAAACATTGCACCTTCGTCCGCCCGAGTGCATAGTCTCTCAAAGACAGTTGGTGGAGATCTGGGAAGGCCTTATTGGAAAGAAACTAGAAAAGGTTACCCTTTCCAAAGATGACTTCCTTGCCTCTATGAAAGATATGGACTATGCAAATCAGGTTGGAGCAGGTCATTTCCATGACATGTACTATGAAGGCTGTTTGACAAACTTTGAAATAGGGGAAAATGCCGAAGAAGCTTCAAAGCTTTATCCCGAAGTGAAGTACACTCGCATGGATGAATTCTTGAAAATTTATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

316

Amino Acids

35.36

Weight (kDa)

6.46

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 4 - 312 6.1e-60 NmrA-like family
Epimerase PF01370 8 - 117 4.9e-07 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 12 - 160 2.2e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 806
AccB1I GGYRCC 1 cut(s) 115
AccI GTMKAC 1 cut(s) 222
AccII CGCG 1 cut(s) 494
AccIII TCCGGA 1 cut(s) 276
AciI CCGC 3 cut(s) 494, 577, 674
AclWI GGATC 1 cut(s) 632
AcsI RAATTY 2 cut(s) 929, 939
AcyI GRCGYC 1 cut(s) 116
AfaI GTAC 2 cut(s) 836, 914
AfiI CCNNNNNNNGG 2 cut(s) 125, 190
AflIII ACRYGT 1 cut(s) 831
AgsI TTSAA 5 cut(s) 314, 398, 863, 891, 937
AjiI CACGTC 1 cut(s) 373
AjnI CCWGG 1 cut(s) 400
AjuI GAANNNNNNNTTGG 4 cut(s) 711, 743, 752, 784
AloI GAACNNNNNNTCC 4 cut(s) 519, 520, 551, 552
AluBI AGCT 7 cut(s) 145, 235, 301, 307, 329, 887, 895
AluI AGCT 7 cut(s) 145, 235, 301, 307, 329, 887, 895
Alw26I GTCTC 1 cut(s) 694
AlwI GGATC 1 cut(s) 632
Ama87I CYCGRG 1 cut(s) 677
Aor13HI TCCGGA 1 cut(s) 276
AoxI GGCC 4 cut(s) 85, 110, 127, 721
ApeKI GCWGC 2 cut(s) 80, 298
ApoI RAATTY 2 cut(s) 929, 939
AspLEI GCGC 1 cut(s) 118
AsuHPI GGTGA 1 cut(s) 173
AvaI CYCGRG 1 cut(s) 677
BanI GGYRCC 1 cut(s) 115
BanII GRGCYC 1 cut(s) 181
BbvI GCAGC 2 cut(s) 92, 310
BccI CCATC 2 cut(s) 361, 563
BciT130I CCWGG 1 cut(s) 402
BcoDI GTCTC 1 cut(s) 694
BfaI CTAG 3 cut(s) 77, 236, 740
BfoI RGCGCY 1 cut(s) 119
BfuAI ACCTGC 1 cut(s) 806
BglII AGATCT 1 cut(s) 711
BisI GCNGC 3 cut(s) 81, 299, 495
BlsI GCNGC 3 cut(s) 82, 300, 496
Bme1390I CCNGG 1 cut(s) 402
BmeT110I CYCGRG 1 cut(s) 677
BmgBI CACGTC 1 cut(s) 373
BmiI GGNNCC 2 cut(s) 117, 537
BmrFI CCNGG 1 cut(s) 402
BmsI GCATC 1 cut(s) 442
BpuEI CTTGAG 1 cut(s) 287
BsaAI YACGTR 1 cut(s) 380
BsaHI GRCGYC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 542
BsaWI WCCGGW 1 cut(s) 276
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 190
Bse118I RCCGGY 2 cut(s) 112, 506
Bse1I ACTGG 2 cut(s) 46, 537
Bse3DI GCAATG 2 cut(s) 606, 659
BseAI TCCGGA 1 cut(s) 276
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 1 cut(s) 542
BseGI GGATG 4 cut(s) 459, 601, 607, 931
BseLI CCNNNNNNNGG 2 cut(s) 125, 190
BseMI GCAATG 2 cut(s) 606, 659
BseNI ACTGG 2 cut(s) 46, 537
BseXI GCAGC 2 cut(s) 92, 310
Bsh1236I CGCG 1 cut(s) 494
BshFI GGCC 4 cut(s) 87, 112, 129, 723
BshNI GGYRCC 1 cut(s) 115
BsiHKCI CYCGRG 1 cut(s) 677
BsiSI CCGG 3 cut(s) 113, 277, 507
BslI CCNNNNNNNGG 2 cut(s) 125, 190
BsmAI GTCTC 1 cut(s) 694
BsnI GGCC 4 cut(s) 87, 112, 129, 723
BsoBI CYCGRG 1 cut(s) 677
Bsp1286I GDGCHC 1 cut(s) 181
Bsp13I TCCGGA 1 cut(s) 276
Bsp143I GATC 3 cut(s) 123, 637, 711
BspACI CCGC 3 cut(s) 494, 577, 674
BspANI GGCC 4 cut(s) 87, 112, 129, 723
BspEI TCCGGA 1 cut(s) 276
BspFNI CGCG 1 cut(s) 494
BspLI GGNNCC 2 cut(s) 117, 537
BspMI ACCTGC 1 cut(s) 806
BspPI GGATC 1 cut(s) 632
BspQI GCTCTTC 1 cut(s) 161
BspT107I GGYRCC 1 cut(s) 115
BsrDI GCAATG 2 cut(s) 606, 659
BsrFI RCCGGY 2 cut(s) 112, 506
BsrI ACTGG 2 cut(s) 46, 537
BssAI RCCGGY 2 cut(s) 112, 506
BssECI CCNNGG 1 cut(s) 542
BssMI GATC 3 cut(s) 123, 637, 711
BssNI GRCGYC 1 cut(s) 116
BssT1I CCWWGG 1 cut(s) 542
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 1 cut(s) 702
Bst6I CTCTTC 2 cut(s) 161, 400
BstACI GRCGYC 1 cut(s) 116
BstAPI GCANNNNNTGC 1 cut(s) 516
BstBAI YACGTR 1 cut(s) 380
BstC8I GCNNGC 4 cut(s) 73, 85, 114, 508
BstEII GGTNACC 1 cut(s) 748
BstENI CCTNNNNNAGG 1 cut(s) 188
BstF5I GGATG 4 cut(s) 459, 601, 607, 931
BstFNI CGCG 1 cut(s) 494
BstH2I RGCGCY 1 cut(s) 119
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 3 cut(s) 126, 640, 714
BstMAI GTCTC 1 cut(s) 694
BstMBI GATC 3 cut(s) 123, 637, 711
BstMWI GCNNNNNNNGC 8 cut(s) 77, 151, 304, 349, 503, 516, 617, 884
BstNI CCWGG 1 cut(s) 402
BstNSI RCATGY 1 cut(s) 835
BstPI GGTNACC 1 cut(s) 748
BstSCI CCNGG 1 cut(s) 400
BstUI CGCG 1 cut(s) 494
BstV1I GCAGC 2 cut(s) 92, 310
BstX2I RGATCY 1 cut(s) 711
BstYI RGATCY 1 cut(s) 711
BsuRI GGCC 4 cut(s) 87, 112, 129, 723
BtrI CACGTC 1 cut(s) 373
BtsCI GGATG 4 cut(s) 459, 601, 607, 931
BtsIMutI CAGTG 1 cut(s) 530
BveI ACCTGC 1 cut(s) 806
Cac8I GCNNGC 4 cut(s) 73, 85, 114, 508
CfoI GCGC 1 cut(s) 118
Cfr10I RCCGGY 2 cut(s) 112, 506
CseI GACGC 1 cut(s) 233
Csp6I GTAC 2 cut(s) 835, 913
CviAII CATG 7 cut(s) 49, 259, 367, 503, 827, 832, 922
CviQI GTAC 2 cut(s) 835, 913
DinI GGCGCC 1 cut(s) 117
DpnI GATC 3 cut(s) 125, 639, 713
DpnII GATC 3 cut(s) 123, 637, 711
Eam1104I CTCTTC 2 cut(s) 161, 400
EarI CTCTTC 2 cut(s) 161, 400
EciI GGCGGA 1 cut(s) 663
Eco130I CCWWGG 1 cut(s) 542
Eco147I AGGCCT 1 cut(s) 723
Eco24I GRGCYC 1 cut(s) 181
Eco88I CYCGRG 1 cut(s) 677
Eco91I GGTNACC 1 cut(s) 748
EcoNI CCTNNNNNAGG 1 cut(s) 188
EcoO65I GGTNACC 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 929
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 542
EcoT38I GRGCYC 1 cut(s) 181
EgeI GGCGCC 1 cut(s) 117
EheI GGCGCC 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 542
FaeI CATG 7 cut(s) 52, 262, 370, 506, 830, 835, 925
FalI AAGNNNNNCTT 4 cut(s) 332, 364, 573, 605
FatI CATG 7 cut(s) 48, 258, 366, 502, 826, 831, 921
FauNDI CATATG 2 cut(s) 251, 472
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 3 cut(s) 81, 299, 495
FokI GGATG 4 cut(s) 446, 608, 614, 938
FriOI GRGCYC 1 cut(s) 181
Fsp4HI GCNGC 3 cut(s) 81, 299, 495
FspBI CTAG 3 cut(s) 77, 236, 740
GlaI GCGC 1 cut(s) 117
GluI GCNGC 3 cut(s) 81, 299, 495
HaeII RGCGCY 1 cut(s) 119
HaeIII GGCC 4 cut(s) 87, 112, 129, 723
HapII CCGG 3 cut(s) 113, 277, 507
HgaI GACGC 1 cut(s) 233
HhaI GCGC 1 cut(s) 118
Hin1I GRCGYC 1 cut(s) 116
Hin1II CATG 7 cut(s) 52, 262, 370, 506, 830, 835, 925
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HindIII AAGCTT 3 cut(s) 305, 885, 893
HinfI GANTC 1 cut(s) 280
HpaII CCGG 3 cut(s) 113, 277, 507
HphI GGTGA 1 cut(s) 173
Hpy166II GTNNAC 2 cut(s) 223, 915
Hpy188I TCNGA 2 cut(s) 205, 358
Hpy188III TCNNGA 7 cut(s) 211, 264, 277, 395, 593, 902, 934
Hpy8I GTNNAC 2 cut(s) 223, 915
Hpy99I CGWCG 3 cut(s) 191, 224, 227
HpyAV CCTTC 5 cut(s) 51, 475, 677, 713, 836
HpyCH4III ACNGT 1 cut(s) 702
HpyCH4IV ACGT 2 cut(s) 372, 379
HpyCH4V TGCA 7 cut(s) 106, 255, 298, 611, 664, 684, 800
HpyF10VI GCNNNNNNNGC 8 cut(s) 77, 151, 304, 349, 503, 516, 617, 884
HpySE526I ACGT 2 cut(s) 372, 379
Hsp92I GRCGYC 1 cut(s) 116
Hsp92II CATG 7 cut(s) 52, 262, 370, 506, 830, 835, 925
HspAI GCGC 1 cut(s) 116
KasI GGCGCC 1 cut(s) 115
Kpn2I TCCGGA 1 cut(s) 276
KroI GCCGGC 2 cut(s) 112, 506
KroNI GCCGGC 2 cut(s) 114, 508
Kzo9I GATC 3 cut(s) 123, 637, 711
LguI GCTCTTC 1 cut(s) 161
LmnI GCTCC 1 cut(s) 812
Lsp1109I GCAGC 2 cut(s) 92, 310
LweI GCATC 1 cut(s) 442
MaeI CTAG 3 cut(s) 77, 236, 740
MaeII ACGT 2 cut(s) 372, 379
MaeIII GTNAC 3 cut(s) 44, 281, 748
MalI GATC 3 cut(s) 125, 639, 713
MboI GATC 3 cut(s) 123, 637, 711
MboII GAAGA 4 cut(s) 148, 417, 461, 893
MflI RGATCY 1 cut(s) 711
MhlI GDGCHC 1 cut(s) 181
MluCI AATT 3 cut(s) 432, 929, 939
Mly113I GGCGCC 1 cut(s) 116
MlyI GAGTC 1 cut(s) 289
MmeI TCCRAC 1 cut(s) 790
MnlI CCTC 8 cut(s) 140, 184, 194, 227, 524, 549, 592, 787
MroI TCCGGA 1 cut(s) 276
MroNI GCCGGC 2 cut(s) 112, 506
MseI TTAA 5 cut(s) 165, 321, 339, 650, 949
MslI CAYNNNNRTG 4 cut(s) 371, 475, 825, 920
MspI CCGG 3 cut(s) 113, 277, 507
MspR9I CCNGG 1 cut(s) 402
MvaI CCWGG 1 cut(s) 402
MvnI CGCG 1 cut(s) 494
MwoI GCNNNNNNNGC 8 cut(s) 77, 151, 304, 349, 503, 516, 617, 884
NaeI GCCGGC 2 cut(s) 114, 508
NarI GGCGCC 1 cut(s) 116
NdeI CATATG 2 cut(s) 251, 472
NdeII GATC 3 cut(s) 123, 637, 711
NgoMIV GCCGGC 2 cut(s) 112, 506
NlaIII CATG 7 cut(s) 52, 262, 370, 506, 830, 835, 925
NlaIV GGNNCC 2 cut(s) 117, 537
NmuCI GTSAC 1 cut(s) 281
NspI RCATGY 1 cut(s) 835
PceI AGGCCT 1 cut(s) 723
PciI ACATGT 1 cut(s) 831
PciSI GCTCTTC 1 cut(s) 161
PdiI GCCGGC 2 cut(s) 114, 508
PflFI GACNNNGTC 1 cut(s) 227
PkrI GCNGC 3 cut(s) 82, 300, 496
PleI GAGTC 1 cut(s) 288
PluTI GGCGCC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 288
Ppu21I YACGTR 1 cut(s) 380
PscI ACATGT 1 cut(s) 831
Psp6I CCWGG 1 cut(s) 400
PspEI GGTNACC 1 cut(s) 748
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 2 cut(s) 117, 537
PsuI RGATCY 1 cut(s) 711
PsyI GACNNNGTC 1 cut(s) 227
RsaI GTAC 2 cut(s) 836, 914
RsaNI GTAC 2 cut(s) 835, 913
RseI CAYNNNNRTG 4 cut(s) 371, 475, 825, 920
SalI GTCGAC 1 cut(s) 221
SapI GCTCTTC 1 cut(s) 161
SaqAI TTAA 5 cut(s) 165, 321, 339, 650, 949
SatI GCNGC 3 cut(s) 81, 299, 495
Sau3AI GATC 3 cut(s) 123, 637, 711
SchI GAGTC 1 cut(s) 289
ScrFI CCNGG 1 cut(s) 402
SduI GDGCHC 1 cut(s) 181
SfaNI GCATC 1 cut(s) 442
SfoI GGCGCC 1 cut(s) 117
SgrDI CGTCGACG 1 cut(s) 221
SmiMI CAYNNNNRTG 4 cut(s) 371, 475, 825, 920
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 3 cut(s) 432, 929, 939
SseBI AGGCCT 1 cut(s) 723
SsiI CCGC 3 cut(s) 494, 577, 674
SspDI GGCGCC 1 cut(s) 115
SspMI CTAG 3 cut(s) 77, 236, 740
StuI AGGCCT 1 cut(s) 723
StyD4I CCNGG 1 cut(s) 400
StyI CCWWGG 1 cut(s) 542
TaaI ACNGT 1 cut(s) 702
TaiI ACGT 2 cut(s) 375, 382
TaqI TCGA 3 cut(s) 132, 189, 222
TasI AATT 3 cut(s) 432, 929, 939
TatI WGTACW 2 cut(s) 834, 912
TauI GCSGC 1 cut(s) 497
Tru1I TTAA 5 cut(s) 165, 321, 339, 650, 949
Tru9I TTAA 5 cut(s) 165, 321, 339, 650, 949
TscAI CASTG 1 cut(s) 537
TseFI GTSAC 1 cut(s) 281
TseI GCWGC 2 cut(s) 80, 298
Tsp45I GTSAC 1 cut(s) 281
TspDTI ATGAA 4 cut(s) 443, 797, 855, 942
TspGWI ACGGA 1 cut(s) 402
TspRI CASTG 1 cut(s) 537
Tth111I GACNNNGTC 1 cut(s) 227
XagI CCTNNNNNAGG 1 cut(s) 188
XapI RAATTY 2 cut(s) 929, 939
XceI RCATGY 1 cut(s) 835
XmiI GTMKAC 1 cut(s) 222
XspI CTAG 3 cut(s) 77, 236, 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.