Rh7DG278500

Isoflavone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0001108
Physical Location & Seq
Reverse (-)
30050025 .. 30051650
1626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG278500.1

Sequence Viewer

Length: 825 bp
ATGGCAAATGCAAAGAGCAACCTTAAGGTTCTTGTGGTAGGTGGTACTGGATATATTGGGAGAAGGATTGTTAGTGCAAGCCTAGCACAAGGCTACCCAACTTATGTCCTTCAAAGGCCGGCAACGGAGATTGGCCTCGATATTGAGAAACTACAAATGCTCTTGTCGTTTAAGAAGCAAGGGGCTCATCTCGTGGAGGGTTCGTTTTCTGATATCCAAAGCCTTGTCGATGCCGTGAAACAAGTTGACGTTGTCATTTGCACCATGTCGGGGGTGCATTTCCGGAGTCACAACCTTTTGTTGCAGCTCAAGCTTGTTGAAGCCATTAAACAAGCTGGAAATGTTAAGCGCTTCTTGCCATCAGAGTTTGGTTTGGACCCGGCGCGTATGGGACATGCACTTGAACCAGGAAGAGTAACATTTGATGAGAAAATGGTGGTGAGAAAGGCAATAGAAGACGCCAACATCCCCTTCACTTATGTGTGTGGTGCTGGCTTCGCCGGTTATTTTGCGGGCAATCTCTCTCAGATGGGAACACTTGTTCCTCCGAAGGACAAAGTCCTTATTTATGGAGATGGCAACACTAAAGTATCTTTCATGGACGAAGATGACATCGGAAGCTATACAATCAAGACAATAGATGATCCGCGAACTTGCAACAAAACATTGTATCTTCTTCCGCCCGAAAACCTACTCACTCAAAGACAATTGGTGGACATTTGGGAAAATCTTGTTGGAAAGAAACTAGAGAAAATTTCCCTTTCTAAAGAAGACCTCCTTGCCTCTATGAAAAGTAAGAGTACTAAAGTGTTGCAAGGTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

274

Amino Acids

30.07

Weight (kDa)

8.8

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 9 - 262 2.4e-59 NmrA-like family
Epimerase PF01370 10 - 119 8.7e-09 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 14 - 164 1.1e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 385, 649
AccIII TCCGGA 1 cut(s) 282
AciI CCGC 3 cut(s) 512, 647, 680
AclWI GGATC 1 cut(s) 638
AcsI RAATTY 1 cut(s) 753
AcyI GRCGYC 1 cut(s) 459
AfaI GTAC 2 cut(s) 46, 802
AfeI AGCGCT 1 cut(s) 350
AfiI CCNNNNNNNGG 1 cut(s) 270
AflII CTTAAG 1 cut(s) 23
AgsI TTSAA 3 cut(s) 113, 320, 404
AjnI CCWGG 1 cut(s) 406
AjuI GAANNNNNNNTTGG 4 cut(s) 455, 487, 717, 749
AleI CACNNNNGTG 1 cut(s) 479
AloI GAACNNNNNNTCC 4 cut(s) 525, 526, 557, 558
AluBI AGCT 5 cut(s) 307, 313, 335, 621, 822
AluI AGCT 5 cut(s) 307, 313, 335, 621, 822
AlwI GGATC 1 cut(s) 638
Aor13HI TCCGGA 1 cut(s) 282
Aor51HI AGCGCT 1 cut(s) 350
AoxI GGCC 2 cut(s) 116, 133
ApeKI GCWGC 1 cut(s) 304
ApoI RAATTY 1 cut(s) 753
AspLEI GCGC 2 cut(s) 351, 385
AspS9I GGNCC 1 cut(s) 376
AsuC2I CCSGG 1 cut(s) 380
AsuHPI GGTGA 1 cut(s) 451
AvaII GGWCC 1 cut(s) 376
BaeI ACNNNNGTAYC 2 cut(s) 573, 606
BanII GRGCYC 1 cut(s) 187
BauI CACGAG 1 cut(s) 191
BbsI GAAGAC 2 cut(s) 462, 777
BbvI GCAGC 1 cut(s) 316
BccI CCATC 3 cut(s) 367, 523, 569
BceAI ACGGC 1 cut(s) 218
BciT130I CCWGG 1 cut(s) 408
BcnI CCSGG 1 cut(s) 380
BfaI CTAG 3 cut(s) 83, 746, 823
BfoI RGCGCY 1 cut(s) 352
BfrI CTTAAG 1 cut(s) 23
BisI GCNGC 1 cut(s) 305
BlsI GCNGC 1 cut(s) 306
BmcAI AGTACT 1 cut(s) 802
Bme1390I CCNGG 2 cut(s) 380, 408
Bme18I GGWCC 1 cut(s) 376
BmgT120I GGNCC 1 cut(s) 376
BmiI GGNNCC 1 cut(s) 378
BmrFI CCNGG 2 cut(s) 380, 408
BmsI GCATC 1 cut(s) 220
BpiI GAAGAC 2 cut(s) 462, 777
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 380
BsaHI GRCGYC 1 cut(s) 459
BsaWI WCCGGW 1 cut(s) 282
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse118I RCCGGY 2 cut(s) 118, 500
Bse1I ACTGG 1 cut(s) 52
BseAI TCCGGA 1 cut(s) 282
BseBI CCWGG 1 cut(s) 408
BseGI GGATG 1 cut(s) 465
BseLI CCNNNNNNNGG 1 cut(s) 270
BseMII CTCAG 1 cut(s) 539
BseNI ACTGG 1 cut(s) 52
BseXI GCAGC 1 cut(s) 316
Bsh1236I CGCG 2 cut(s) 385, 649
BshFI GGCC 2 cut(s) 118, 135
BsiSI CCGG 4 cut(s) 119, 283, 380, 501
BslFI GGGAC 1 cut(s) 405
BslI CCNNNNNNNGG 1 cut(s) 270
BsmFI GGGAC 1 cut(s) 405
BsnI GGCC 2 cut(s) 118, 135
Bsp1286I GDGCHC 1 cut(s) 187
Bsp13I TCCGGA 1 cut(s) 282
Bsp143I GATC 1 cut(s) 643
BspACI CCGC 3 cut(s) 512, 647, 680
BspANI GGCC 2 cut(s) 118, 135
BspCNI CTCAG 1 cut(s) 538
BspEI TCCGGA 1 cut(s) 282
BspFNI CGCG 2 cut(s) 385, 649
BspLI GGNNCC 1 cut(s) 378
BspPI GGATC 1 cut(s) 638
BspTI CTTAAG 1 cut(s) 23
BsrFI RCCGGY 2 cut(s) 118, 500
BsrI ACTGG 1 cut(s) 52
BssAI RCCGGY 2 cut(s) 118, 500
BssMI GATC 1 cut(s) 643
BssNI GRCGYC 1 cut(s) 459
BssSI CACGAG 1 cut(s) 191
Bst2BI CACGAG 1 cut(s) 191
Bst2UI CCWGG 1 cut(s) 408
Bst6I CTCTTC 1 cut(s) 406
BstACI GRCGYC 1 cut(s) 459
BstAFI CTTAAG 1 cut(s) 23
BstC8I GCNNGC 4 cut(s) 79, 120, 493, 514
BstDEI CTNAG 1 cut(s) 525
BstF5I GGATG 1 cut(s) 465
BstFNI CGCG 2 cut(s) 385, 649
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 2 cut(s) 351, 385
BstKTI GATC 1 cut(s) 646
BstMBI GATC 1 cut(s) 643
BstMWI GCNNNNNNNGC 4 cut(s) 83, 310, 355, 497
BstNI CCWGG 1 cut(s) 408
BstNSI RCATGY 1 cut(s) 398
BstSCI CCNGG 2 cut(s) 378, 406
BstUI CGCG 2 cut(s) 385, 649
BstV1I GCAGC 1 cut(s) 316
BstV2I GAAGAC 2 cut(s) 462, 777
BsuRI GGCC 2 cut(s) 118, 135
BtsCI GGATG 1 cut(s) 465
Cac8I GCNNGC 4 cut(s) 79, 120, 493, 514
CfoI GCGC 2 cut(s) 351, 385
Cfr10I RCCGGY 2 cut(s) 118, 500
Cfr13I GGNCC 1 cut(s) 376
CseI GACGC 1 cut(s) 467
Csp6I GTAC 2 cut(s) 45, 801
CviAII CATG 3 cut(s) 265, 395, 598
CviQI GTAC 2 cut(s) 45, 801
DdeI CTNAG 1 cut(s) 525
DpnI GATC 1 cut(s) 645
DpnII GATC 1 cut(s) 643
Eam1104I CTCTTC 1 cut(s) 406
EarI CTCTTC 1 cut(s) 406
EciI GGCGGA 1 cut(s) 669
Eco24I GRGCYC 1 cut(s) 187
Eco32I GATATC 1 cut(s) 214
Eco47I GGWCC 1 cut(s) 376
Eco47III AGCGCT 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 406
EcoRV GATATC 1 cut(s) 214
EcoT38I GRGCYC 1 cut(s) 187
FaeI CATG 3 cut(s) 268, 398, 601
FalI AAGNNNNNCTT 4 cut(s) 338, 370, 762, 794
FaqI GGGAC 1 cut(s) 405
FatI CATG 3 cut(s) 264, 394, 597
FauI CCCGC 1 cut(s) 505
Fnu4HI GCNGC 1 cut(s) 305
FokI GGATG 1 cut(s) 452
FriOI GRGCYC 1 cut(s) 187
Fsp4HI GCNGC 1 cut(s) 305
FspBI CTAG 3 cut(s) 83, 746, 823
GlaI GCGC 2 cut(s) 350, 384
GluI GCNGC 1 cut(s) 305
HaeII RGCGCY 1 cut(s) 352
HaeIII GGCC 2 cut(s) 118, 135
HapII CCGG 4 cut(s) 119, 283, 380, 501
HgaI GACGC 1 cut(s) 467
HhaI GCGC 2 cut(s) 351, 385
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 3 cut(s) 268, 398, 601
Hin6I GCGC 2 cut(s) 349, 383
HinP1I GCGC 2 cut(s) 349, 383
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 1 cut(s) 286
HpaII CCGG 4 cut(s) 119, 283, 380, 501
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 2 cut(s) 247, 715
Hpy188I TCNGA 5 cut(s) 211, 364, 528, 549, 617
Hpy188III TCNNGA 2 cut(s) 283, 631
Hpy8I GTNNAC 2 cut(s) 247, 715
HpyAV CCTTC 4 cut(s) 57, 119, 481, 544
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 8 cut(s) 11, 77, 261, 277, 304, 398, 657, 814
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 310, 355, 497
HpyF3I CTNAG 1 cut(s) 525
HpySE526I ACGT 1 cut(s) 249
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 3 cut(s) 268, 398, 601
HspAI GCGC 2 cut(s) 349, 383
Kpn2I TCCGGA 1 cut(s) 282
KroI GCCGGC 1 cut(s) 118
KroNI GCCGGC 1 cut(s) 120
Kzo9I GATC 1 cut(s) 643
LpnPI CCDG 9 cut(s) 33, 132, 296, 321, 393, 393, 420, 477, 514
Lsp1109I GCAGC 1 cut(s) 316
LweI GCATC 1 cut(s) 220
MaeI CTAG 3 cut(s) 83, 746, 823
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 2 cut(s) 287, 415
MalI GATC 1 cut(s) 645
MboI GATC 1 cut(s) 643
MboII GAAGA 6 cut(s) 423, 467, 617, 665, 668, 782
MfeI CAATTG 1 cut(s) 707
MhlI GDGCHC 1 cut(s) 187
MluCI AATT 2 cut(s) 707, 753
MlyI GAGTC 1 cut(s) 295
MmeI TCCRAC 1 cut(s) 715
MnlI CCTC 5 cut(s) 146, 190, 555, 785, 793
MroI TCCGGA 1 cut(s) 282
MroNI GCCGGC 1 cut(s) 118
MseI TTAA 4 cut(s) 24, 171, 327, 345
MslI CAYNNNNRTG 1 cut(s) 479
MspCI CTTAAG 1 cut(s) 23
MspI CCGG 4 cut(s) 119, 283, 380, 501
MspR9I CCNGG 2 cut(s) 380, 408
MunI CAATTG 1 cut(s) 707
MvaI CCWGG 1 cut(s) 408
MvnI CGCG 2 cut(s) 385, 649
MwoI GCNNNNNNNGC 4 cut(s) 83, 310, 355, 497
NaeI GCCGGC 1 cut(s) 120
NciI CCSGG 1 cut(s) 380
NdeII GATC 1 cut(s) 643
NgoMIV GCCGGC 1 cut(s) 118
NlaIII CATG 3 cut(s) 268, 398, 601
NlaIV GGNNCC 1 cut(s) 378
NmuCI GTSAC 1 cut(s) 287
NspI RCATGY 1 cut(s) 398
OliI CACNNNNGTG 1 cut(s) 479
PdiI GCCGGC 1 cut(s) 120
PflFI GACNNNGTC 2 cut(s) 251, 557
PkrI GCNGC 1 cut(s) 306
PleI GAGTC 1 cut(s) 294
PpsI GAGTC 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 406
PspN4I GGNNCC 1 cut(s) 378
PspPI GGNCC 1 cut(s) 376
PsyI GACNNNGTC 2 cut(s) 251, 557
RsaI GTAC 2 cut(s) 46, 802
RsaNI GTAC 2 cut(s) 45, 801
RseI CAYNNNNRTG 1 cut(s) 479
SaqAI TTAA 4 cut(s) 24, 171, 327, 345
SatI GCNGC 1 cut(s) 305
Sau3AI GATC 1 cut(s) 643
Sau96I GGNCC 1 cut(s) 376
ScaI AGTACT 1 cut(s) 802
SchI GAGTC 1 cut(s) 295
ScrFI CCNGG 2 cut(s) 380, 408
SduI GDGCHC 1 cut(s) 187
SfaNI GCATC 1 cut(s) 220
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 1 cut(s) 479
SmlI CTYRAG 2 cut(s) 23, 308
SmoI CTYRAG 2 cut(s) 23, 308
Sse9I AATT 2 cut(s) 707, 753
SsiI CCGC 3 cut(s) 512, 647, 680
SspMI CTAG 3 cut(s) 83, 746, 823
StyD4I CCNGG 2 cut(s) 378, 406
TaiI ACGT 1 cut(s) 252
TaqI TCGA 2 cut(s) 138, 228
TasI AATT 2 cut(s) 707, 753
TatI WGTACW 1 cut(s) 800
Tru1I TTAA 4 cut(s) 24, 171, 327, 345
Tru9I TTAA 4 cut(s) 24, 171, 327, 345
TseFI GTSAC 1 cut(s) 287
TseI GCWGC 1 cut(s) 304
Tsp45I GTSAC 1 cut(s) 287
TspDTI ATGAA 2 cut(s) 586, 803
TspGWI ACGGA 1 cut(s) 140
Tth111I GACNNNGTC 2 cut(s) 251, 557
Vha464I CTTAAG 1 cut(s) 23
VpaK11BI GGWCC 1 cut(s) 376
XapI RAATTY 1 cut(s) 753
XceI RCATGY 1 cut(s) 398
XspI CTAG 3 cut(s) 83, 746, 823
ZrmI AGTACT 1 cut(s) 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.