FvH4_5g34680

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001339
Physical Location & Seq
Reverse (-)
25394368 .. 25400616
6249 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g34680.t2

Sequence Viewer

Length: 2871 bp
ATGGCGACTACTGTAGCGTCTCTAGTACTTTCGACCGGCATCACATCGGCGGTTCTTCAGGTTCTTGTCGACAGAATCGCCACCATCACTCAAACAAAGATAACCCAGTTTAAGCAGCTGCAAAGCCAGTTCTTGGAGTTAGGAGCAACAATGACCAAGGCAAAAGCTCTGGTTGACGATGTAGAAAACAAGAAATGTTTTCCGGAGGCGTGCCGTATTTTGCTGGAAGATATCAAAAGTGTTGTTTTAGATGCCGATGACTTGCTGGATGAGATTGACATGTTTCTCGAAAACAGAACCGATAAGAATCAGGTGAGTCAAATCACTCAAATGTTCCCATCATCTTTTAGATTCAATATTCCTTCTGAGCTGCGCAAGATCCATAAAGAATTGGGTGATTTAGTAGATAAACTGGAGGGCTACTGCATGATGAATTTGGGTCAGCCTCAAATGGCTGCTCCCACTCCCATTTTGCCAAGTACTTCGTTGGTAGTTGGGTCCTCTATAATTTCAAGAGATCCAGACAAGAAAAAGATCATTGAGATGGTCTTGTCTGACCAAGGAGAGGGTGGAGGAAATGTTTCTGTGATTCCCATAGTGGGAATGGGTGGAATTGGTAAAACTGCACTTGCTCAACTTGTTTACAATGATCCGGATGTGGTTAGAAATTTTGATTTTAAGATCTGGGTATCAGTTTCTTTCAGCTATGATGTTGTCATGATTACAAAATCCATTTTTAACTCTGTTGCTGAGCAAAGGTCTGGGTTAATGTCTGAATTTAGGGGAGTTTTTGAGTTGTCTGATATGTGGAAATATTTTGAGTTGAGTCATAATGAGATTCATATTCAGTTACAGAAGAAGTTGAGGGGGAAGAAGTTTTTGCTTGTACTTGACGATATGTGGAATGAAAATCCTAGAGATTGGGATCAATTGAAATTGCCTTTTCAAGTTGCGGCACAAGGAAGCAAGATTTTGATGACTACACGAAGCCAAATTGCTTCGTCTATTCTTTCCGGTGCTCCTGCTTATCTTTTGAATACTTTGTCTCATAAGGATTGCTGGACAATCATTAAACAAACAGCAGGACTAGGTGATGCACATGAAAATGAGTTGATCGGGTTGGAGATAGCAAAGAAGTGCAAAGGGCTTCCTTTGGCGGCAAAAGTGATTGGAAGTGTCTTGCGTTATAAATATGGAGAAAGGAAGTGGGGTGCTTTGTTAAAATGTGAGCTATGGGAATCTCCAGAACATGGAAACCATATATATCCAGCACTTAAGTTGAGCTATGATAATTTGTCTTCTTATTTGAGAAGATGTTTCACTTATTGTTCTATCTTCCCACGTGATCATGAATTTGAGAAAGATGATTTGGTTCAGTTATGGGCTGCAGAAGGCTTCATTCAAGCTCAAGGAAGACAAGAAATTGAAGATGTTGGAAGAGAGTACTTTGGTGGCTTGTGTTCCAGGTCCTTTTTCCAAGCTTCAGATGATGATCCACCAAAATATAAAATGCATGACCTCGTTCATGATTTGGCAAGATTGGTTTCAACCACTGTTTCCTTTTCCATGGAGGATAATGTGTCAAACATCTTACCGATACCTAGACATGCTCGGTATTCATCATTGCTTTGTCAAGACATTAAGCCATCTACATTGGAGGTGTTTCACAGGTATGAGAAGTTGCGAACCTTTTTGCTGCTTTCTGAACATGCATCCAATCTTAAACAAGTTCCATATGATTTCTTTATAAAGTTGGGACGCTTAAGAGTGTTGAATCTGAGCCATAGTGGCATCTCTGACTTGCCTGACTCTGTTAAGAATTTGATACACCTTCGTTACCTGAATCTTAGTCATTCCTCTATCAAAAGATTGCCAAAATCTTTAGCAAATATATATGGGATGGAGACACTTAAGCTCAAGAATTGTTGTGAGCTTCTTCAATTACCTGACAACTTGAAGAACTTGATTAAGTTGCGACATCTTGACTTTGATGGTCGTGTAATCAGTTCCATGCCAATGGATGTTGGGAAGCTGACCAGTCTTGAAACTCTGCCTGCATTTATAGTGGGAAAGCACATTGGATATCAGATTGGAGAGCTCAAGAACATGATGTTCCTTCGTGGATCCATTTGCATTACAAATCTTGAAAATGTGGCAAATTCTGTTGAGGGTGAGGCTGCTATGTTACATGAGAAGCAATACCTAAAGAAGTTGGAGTTGGAATGGAATGAAATGGGTTCTCAAACAGTTCATCAAGAAGTTCTTACGAGCCTTAAGCCTCATGTTAGGTTGACAGAGTTACGAGTAACAGGCTATTGTGGTTTAATATTTCCAAATTGGATTTCTGATTCATCATTTTGCAAGCTTGAGAGAATCCATTTGGACAAGTGCCATTTTTGCACACTGCTTCCTTCTCTTGGTCAACTTACAGCTCTCAAGTATCTTCTCATCCAAGATATGCATGATTTGGAAAATATTGATCATCTGTTATGTGGCTCAACAGATACTTTTCCTTCATTGGAGACGCTGACATTTCGGGACATGCCAAAGTTGAAGATATGGTCAGGAATAAATGAAAATGACATGCCTCACCTTATAGTGCTTAGCATTGATTCTTGCCCAGAACTGGTCACGCTACCTTCATTGTGTTACCTAAGATCACTTGAAATGTTAGAAATAAACCGTTGCTCGGAGCTTCAATCATTGCCAAGGGAAGGACTGCCGCTCTCACTCACATGTTTGATAATTTCGGAAAGTGACATTTTAAAGGAGCGATGCGAGGTGCAAGGTGCAGATTGGGACAAGATCAAATCGATTCCCAACATAATGATAGATGATGAAGTGATTCCCATGGAAGGACGTCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

957

Amino Acids

108.22

Weight (kDa)

5.95

Isoelectric Point (pI)

38.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 18 - 102 1.7e-08 Rx N-terminal domain
NB-ARC PF00931 191 - 361 2.5e-30 NB-ARC domain
WHD_DRP PF23559 445 - 512 1.5e-27 Disease resistance protein Winged helix domain
LRR_8 PF13855 572 - 620 7.2e-06 Leucine rich repeat
LRR_14 PF23598 586 - 872 4.2e-26 Leucine-rich repeat region
LRR_8 PF13855 587 - 642 3.3e-06 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 697 - 820 5.2e-35 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 782 - 896 3.1e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1188, 1748
AatII GACGTC 1 cut(s) 2863
Acc16I TGCGCA 1 cut(s) 374
AccB7I CCANNNNNTGG 2 cut(s) 133, 1250
AccBSI CCGCTC 1 cut(s) 2725
AccI GTMKAC 1 cut(s) 69
AccIII TCCGGA 2 cut(s) 202, 652
AciI CCGC 4 cut(s) 50, 953, 1157, 2723
AclWI GGATC 7 cut(s) 373, 512, 644, 933, 1487, 2118, 2131
AcsI RAATTY 6 cut(s) 433, 667, 776, 1352, 1819, 2158
AcuI CTGAAG 2 cut(s) 41, 1467
AcvI CACGTG 1 cut(s) 1343
AcyI GRCGYC 1 cut(s) 2860
AfaI GTAC 4 cut(s) 27, 481, 888, 1445
AfiI CCNNNNNNNGG 9 cut(s) 133, 565, 599, 1250, 2417, 2626, 2689, 2714, 2855
AflII CTTAAG 4 cut(s) 1274, 1762, 1910, 2273
AflIII ACRYGT 2 cut(s) 279, 2735
AjnI CCWGG 1 cut(s) 1463
AjuI GAANNNNNNNTTGG 6 cut(s) 1352, 1384, 2201, 2233, 2326, 2358
Alw21I GWGCWC 2 cut(s) 1021, 2100
Alw26I GTCTC 4 cut(s) 24, 1050, 1898, 2516
AlwI GGATC 7 cut(s) 373, 512, 644, 933, 1487, 2118, 2131
Aor13HI TCCGGA 2 cut(s) 202, 652
ApeKI GCWGC 7 cut(s) 115, 118, 370, 455, 1385, 1696, 2177
ApoI RAATTY 6 cut(s) 433, 667, 776, 1352, 1819, 2158
Asp700I GAANNNNTTC 2 cut(s) 580, 2844
AspLEI GCGC 1 cut(s) 375
AspS9I GGNCC 2 cut(s) 498, 1467
AsuHPI GGTGA 5 cut(s) 325, 407, 1103, 2183, 2582
AvaII GGWCC 2 cut(s) 498, 1467
BamHI GGATCC 1 cut(s) 2123
BanII GRGCYC 1 cut(s) 2100
BbrPI CACGTG 1 cut(s) 1343
BbsI GAAGAC 2 cut(s) 1290, 1420
Bbv12I GWGCWC 2 cut(s) 1021, 2100
BbvI GCAGC 7 cut(s) 105, 127, 357, 442, 1372, 1683, 2164
BccI CCATC 6 cut(s) 92, 346, 538, 1654, 1894, 1985
BceAI ACGGC 1 cut(s) 198
BciT130I CCWGG 1 cut(s) 1465
BclI TGATCA 2 cut(s) 1345, 2479
BcoDI GTCTC 4 cut(s) 24, 1050, 1898, 2516
BfaI CTAG 4 cut(s) 23, 915, 1088, 1602
BfmI CTRYAG 2 cut(s) 12, 1386
BfrI CTTAAG 4 cut(s) 1274, 1762, 1910, 2273
BglI GCCNNNNNGGC 1 cut(s) 1788
BglII AGATCT 1 cut(s) 681
BlpI GCTNAGC 2 cut(s) 750, 2603
BmcAI AGTACT 3 cut(s) 27, 481, 1445
Bme1390I CCNGG 1 cut(s) 1465
Bme18I GGWCC 2 cut(s) 498, 1467
BmgT120I GGNCC 2 cut(s) 498, 1467
BmiI GGNNCC 2 cut(s) 499, 2125
BmrFI CCNGG 1 cut(s) 1465
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 6 cut(s) 48, 241, 1084, 1721, 1800, 2765
BmuI ACTGGG 1 cut(s) 100
BpiI GAAGAC 2 cut(s) 1290, 1420
BpmI CTGGAG 2 cut(s) 434, 1227
Bpu1102I GCTNAGC 2 cut(s) 750, 2603
BpuEI CTTGAG 5 cut(s) 1392, 1901, 2084, 2387, 2420
Bsa29I ATCGAT 1 cut(s) 2813
BsaAI YACGTR 1 cut(s) 1343
BsaBI GATNNNNATC 3 cut(s) 306, 924, 1491
BsaHI GRCGYC 1 cut(s) 2860
BsaJI CCNNGG 5 cut(s) 156, 559, 1566, 2708, 2850
BsaWI WCCGGW 3 cut(s) 202, 652, 1013
BsaXI ACNNNNNCTCC 2 cut(s) 564, 594
Bsc4I CCNNNNNNNGG 9 cut(s) 133, 565, 599, 1250, 2417, 2626, 2689, 2714, 2855
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 5 cut(s) 106, 127, 417, 2037, 2631
Bse3DI GCAATG 2 cut(s) 1622, 2702
Bse8I GATNNNNATC 3 cut(s) 306, 924, 1491
BseAI TCCGGA 2 cut(s) 202, 652
BseBI CCWGG 1 cut(s) 1465
BseCI ATCGAT 1 cut(s) 2813
BseDI CCNNGG 5 cut(s) 156, 559, 1566, 2708, 2850
BseGI GGATG 6 cut(s) 274, 661, 1712, 1905, 2026, 2448
BseJI GATNNNNATC 3 cut(s) 306, 924, 1491
BseLI CCNNNNNNNGG 9 cut(s) 133, 565, 599, 1250, 2417, 2626, 2689, 2714, 2855
BseMI GCAATG 2 cut(s) 1622, 2702
BseMII CTCAG 3 cut(s) 357, 741, 1769
BseNI ACTGG 5 cut(s) 106, 127, 417, 2037, 2631
BseXI GCAGC 7 cut(s) 105, 127, 357, 442, 1372, 1683, 2164
BsgI GTGCAG 2 cut(s) 609, 2811
Bsh1285I CGRYCG 1 cut(s) 36
BshVI ATCGAT 1 cut(s) 2813
BsiEI CGRYCG 1 cut(s) 36
BsiHKAI GWGCWC 2 cut(s) 1021, 2100
BsiSI CCGG 4 cut(s) 36, 203, 653, 1014
BslFI GGGAC 3 cut(s) 1770, 2552, 2813
BslI CCNNNNNNNGG 9 cut(s) 133, 565, 599, 1250, 2417, 2626, 2689, 2714, 2855
BsmAI GTCTC 4 cut(s) 24, 1050, 1898, 2516
BsmBI CGTCTC 2 cut(s) 24, 2516
BsmFI GGGAC 3 cut(s) 1770, 2552, 2813
Bsp1286I GDGCHC 2 cut(s) 1021, 2100
Bsp13I TCCGGA 2 cut(s) 202, 652
Bsp1720I GCTNAGC 2 cut(s) 750, 2603
Bsp19I CCATGG 2 cut(s) 1566, 2850
BspACI CCGC 4 cut(s) 50, 953, 1157, 2723
BspCNI CTCAG 3 cut(s) 358, 742, 1770
BspDI ATCGAT 1 cut(s) 2813
BspEI TCCGGA 2 cut(s) 202, 652
BspHI TCATGA 3 cut(s) 717, 1348, 1525
BspLI GGNNCC 2 cut(s) 499, 2125
BspMAI CTGCAG 1 cut(s) 1390
BspPI GGATC 7 cut(s) 373, 512, 644, 933, 1487, 2118, 2131
BspTI CTTAAG 4 cut(s) 1274, 1762, 1910, 2273
BsrBI CCGCTC 1 cut(s) 2725
BsrDI GCAATG 2 cut(s) 1622, 2702
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 5 cut(s) 106, 127, 417, 2037, 2631
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 5 cut(s) 156, 559, 1566, 2708, 2850
BssNI GRCGYC 1 cut(s) 2860
BssT1I CCWWGG 5 cut(s) 156, 559, 1566, 2708, 2850
Bst2UI CCWGG 1 cut(s) 1465
Bst4CI ACNGT 4 cut(s) 13, 1555, 2248, 2684
Bst6I CTCTTC 1 cut(s) 1432
BstACI GRCGYC 1 cut(s) 2860
BstAFI CTTAAG 4 cut(s) 1274, 1762, 1910, 2273
BstBAI YACGTR 1 cut(s) 1343
BstC8I GCNNGC 3 cut(s) 211, 2055, 2363
BstDEI CTNAG 6 cut(s) 366, 750, 1778, 1847, 2603, 2654
BstDSI CCRYGG 2 cut(s) 1566, 2850
BstF5I GGATG 6 cut(s) 274, 661, 1712, 1905, 2026, 2448
BstHHI GCGC 1 cut(s) 375
BstMAI GTCTC 4 cut(s) 24, 1050, 1898, 2516
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 2 cut(s) 1788, 2397
BstNI CCWGG 1 cut(s) 1465
BstNSI RCATGY 6 cut(s) 283, 1610, 1712, 2545, 2587, 2739
BstSCI CCNGG 1 cut(s) 1463
BstSFI CTRYAG 2 cut(s) 12, 1386
BstV1I GCAGC 7 cut(s) 105, 127, 357, 442, 1372, 1683, 2164
BstV2I GAAGAC 2 cut(s) 1290, 1420
BstX2I RGATCY 4 cut(s) 378, 517, 681, 2123
BstXI CCANNNNNNTGG 1 cut(s) 2017
BstYI RGATCY 4 cut(s) 378, 517, 681, 2123
Bsu15I ATCGAT 1 cut(s) 2813
BsuTUI ATCGAT 1 cut(s) 2813
BtgI CCRYGG 2 cut(s) 1566, 2850
BtgZI GCGATG 1 cut(s) 2788
BtsCI GGATG 6 cut(s) 274, 661, 1712, 1905, 2026, 2448
BtsI GCAGTG 1 cut(s) 2402
BtsIMutI CAGTG 2 cut(s) 1551, 2402
Cac8I GCNNGC 3 cut(s) 211, 2055, 2363
CciI TCATGA 3 cut(s) 717, 1348, 1525
CfoI GCGC 1 cut(s) 375
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 2 cut(s) 498, 1467
ClaI ATCGAT 1 cut(s) 2813
CseI GACGC 3 cut(s) 6, 1767, 2533
Csp6I GTAC 4 cut(s) 26, 480, 887, 1444
CviQI GTAC 4 cut(s) 26, 480, 887, 1444
DdeI CTNAG 6 cut(s) 366, 750, 1778, 1847, 2603, 2654
DraI TTTAAA 1 cut(s) 2766
Eam1104I CTCTTC 1 cut(s) 1432
EarI CTCTTC 1 cut(s) 1432
Ecl136II GAGCTC 1 cut(s) 2098
Eco130I CCWWGG 5 cut(s) 156, 559, 1566, 2708, 2850
Eco24I GRGCYC 1 cut(s) 2100
Eco32I GATATC 2 cut(s) 232, 2084
Eco47I GGWCC 2 cut(s) 498, 1467
Eco53kI GAGCTC 1 cut(s) 2098
Eco57I CTGAAG 2 cut(s) 41, 1467
Eco72I CACGTG 1 cut(s) 1343
EcoICRI GAGCTC 1 cut(s) 2098
EcoO109I RGGNCCY 2 cut(s) 498, 1467
EcoRII CCWGG 1 cut(s) 1463
EcoRV GATATC 2 cut(s) 232, 2084
EcoT14I CCWWGG 5 cut(s) 156, 559, 1566, 2708, 2850
EcoT22I ATGCAT 3 cut(s) 1515, 1714, 2463
EcoT38I GRGCYC 1 cut(s) 2100
ErhI CCWWGG 5 cut(s) 156, 559, 1566, 2708, 2850
Esp3I CGTCTC 2 cut(s) 24, 2516
FalI AAGNNNNNCTT 2 cut(s) 2247, 2279
FaqI GGGAC 3 cut(s) 1770, 2552, 2813
FauNDI CATATG 1 cut(s) 1735
FbaI TGATCA 2 cut(s) 1345, 2479
FblI GTMKAC 1 cut(s) 69
FokI GGATG 6 cut(s) 281, 668, 1699, 1912, 2033, 2435
FriOI GRGCYC 1 cut(s) 2100
FspBI CTAG 4 cut(s) 23, 915, 1088, 1602
FspI TGCGCA 1 cut(s) 374
GlaI GCGC 1 cut(s) 374
GsuI CTGGAG 2 cut(s) 434, 1227
HapII CCGG 4 cut(s) 36, 203, 653, 1014
HgaI GACGC 3 cut(s) 6, 1767, 2533
HhaI GCGC 1 cut(s) 375
Hin1I GRCGYC 1 cut(s) 2860
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HincII GTYRAC 4 cut(s) 70, 175, 2292, 2423
HindII GTYRAC 4 cut(s) 70, 175, 2292, 2423
HindIII AAGCTT 2 cut(s) 1479, 2363
HpaII CCGG 4 cut(s) 36, 203, 653, 1014
HphI GGTGA 5 cut(s) 325, 407, 1103, 2183, 2582
Hpy166II GTNNAC 5 cut(s) 70, 175, 643, 2292, 2423
Hpy8I GTNNAC 5 cut(s) 70, 175, 643, 2292, 2423
HpyAV CCTTC 9 cut(s) 372, 1385, 1841, 2126, 2421, 2523, 2649, 2708, 2849
HpyCH4III ACNGT 4 cut(s) 13, 1555, 2248, 2684
HpyCH4IV ACGT 2 cut(s) 1342, 2860
HpyF10VI GCNNNNNNNGC 2 cut(s) 1788, 2397
HpyF3I CTNAG 6 cut(s) 366, 750, 1778, 1847, 2603, 2654
HpySE526I ACGT 2 cut(s) 1342, 2860
Hsp92I GRCGYC 1 cut(s) 2860
HspAI GCGC 1 cut(s) 373
Kpn2I TCCGGA 2 cut(s) 202, 652
Ksp22I TGATCA 2 cut(s) 1345, 2479
LmnI GCTCC 5 cut(s) 143, 463, 1024, 2692, 2770
Lsp1109I GCAGC 7 cut(s) 105, 127, 357, 442, 1372, 1683, 2164
LweI GCATC 6 cut(s) 48, 241, 1084, 1721, 1800, 2765
MaeI CTAG 4 cut(s) 23, 915, 1088, 1602
MaeII ACGT 2 cut(s) 1342, 2860
MaeIII GTNAC 8 cut(s) 849, 1835, 2184, 2298, 2305, 2629, 2648, 2756
MbiI CCGCTC 1 cut(s) 2725
MfeI CAATTG 1 cut(s) 929
MflI RGATCY 4 cut(s) 378, 517, 681, 2123
MhlI GDGCHC 2 cut(s) 1021, 2100
MlyI GAGTC 3 cut(s) 325, 835, 1802
MmeI TCCRAC 4 cut(s) 1101, 1414, 2193, 2199
Mph1103I ATGCAT 3 cut(s) 1515, 1714, 2463
MroI TCCGGA 2 cut(s) 202, 652
MroXI GAANNNNTTC 2 cut(s) 580, 2844
MslI CAYNNNNRTG 6 cut(s) 329, 542, 1104, 1671, 2015, 2734
MspA1I CMGCKG 1 cut(s) 118
MspCI CTTAAG 4 cut(s) 1274, 1762, 1910, 2273
MspI CCGG 4 cut(s) 36, 203, 653, 1014
MspR9I CCNGG 1 cut(s) 1465
MunI CAATTG 1 cut(s) 929
MvaI CCWGG 1 cut(s) 1465
MwoI GCNNNNNNNGC 2 cut(s) 1788, 2397
NcoI CCATGG 2 cut(s) 1566, 2850
NdeI CATATG 1 cut(s) 1735
NlaIV GGNNCC 2 cut(s) 499, 2125
NmuCI GTSAC 2 cut(s) 2629, 2756
NsbI TGCGCA 1 cut(s) 374
NsiI ATGCAT 3 cut(s) 1515, 1714, 2463
NspI RCATGY 6 cut(s) 283, 1610, 1712, 2545, 2587, 2739
PagI TCATGA 3 cut(s) 717, 1348, 1525
PciI ACATGT 2 cut(s) 279, 2735
PdmI GAANNNNTTC 2 cut(s) 580, 2844
PflMI CCANNNNNTGG 2 cut(s) 133, 1250
PleI GAGTC 3 cut(s) 324, 834, 1802
PmaCI CACGTG 1 cut(s) 1343
PmlI CACGTG 1 cut(s) 1343
PpsI GAGTC 3 cut(s) 324, 834, 1802
Ppu21I YACGTR 1 cut(s) 1343
PpuMI RGGWCCY 2 cut(s) 498, 1467
PscI ACATGT 2 cut(s) 279, 2735
PsiI TTATAA 2 cut(s) 1188, 1748
Psp124BI GAGCTC 1 cut(s) 2100
Psp5II RGGWCCY 2 cut(s) 498, 1467
Psp6I CCWGG 1 cut(s) 1463
PspCI CACGTG 1 cut(s) 1343
PspGI CCWGG 1 cut(s) 1463
PspN4I GGNNCC 2 cut(s) 499, 2125
PspPI GGNCC 2 cut(s) 498, 1467
PspPPI RGGWCCY 2 cut(s) 498, 1467
PstI CTGCAG 1 cut(s) 1390
PsuI RGATCY 4 cut(s) 378, 517, 681, 2123
PvuII CAGCTG 1 cut(s) 118
RsaI GTAC 4 cut(s) 27, 481, 888, 1445
RsaNI GTAC 4 cut(s) 26, 480, 887, 1444
RseI CAYNNNNRTG 6 cut(s) 329, 542, 1104, 1671, 2015, 2734
SacI GAGCTC 1 cut(s) 2100
SalI GTCGAC 1 cut(s) 68
Sau96I GGNCC 2 cut(s) 498, 1467
ScaI AGTACT 3 cut(s) 27, 481, 1445
SchI GAGTC 3 cut(s) 325, 835, 1802
ScrFI CCNGG 1 cut(s) 1465
SduI GDGCHC 2 cut(s) 1021, 2100
SfaNI GCATC 6 cut(s) 48, 241, 1084, 1721, 1800, 2765
SfcI CTRYAG 2 cut(s) 12, 1386
SinI GGWCC 2 cut(s) 498, 1467
SmiMI CAYNNNNRTG 6 cut(s) 329, 542, 1104, 1671, 2015, 2734
SmlI CTYRAG 9 cut(s) 1274, 1407, 1762, 1910, 1916, 2099, 2273, 2366, 2435
SmoI CTYRAG 9 cut(s) 1274, 1407, 1762, 1910, 1916, 2099, 2273, 2366, 2435
SsiI CCGC 4 cut(s) 50, 953, 1157, 2723
SspI AATATT 4 cut(s) 358, 815, 2328, 2476
SspMI CTAG 4 cut(s) 23, 915, 1088, 1602
SstI GAGCTC 1 cut(s) 2100
StyD4I CCNGG 1 cut(s) 1463
StyI CCWWGG 5 cut(s) 156, 559, 1566, 2708, 2850
TaaI ACNGT 4 cut(s) 13, 1555, 2248, 2684
TaiI ACGT 2 cut(s) 1345, 2863
TaqI TCGA 4 cut(s) 32, 69, 288, 2813
TatI WGTACW 4 cut(s) 25, 479, 886, 1443
TauI GCSGC 3 cut(s) 956, 1160, 2725
TscAI CASTG 2 cut(s) 1558, 2409
TseFI GTSAC 2 cut(s) 2629, 2756
TseI GCWGC 7 cut(s) 115, 118, 370, 455, 1385, 1696, 2177
Tsp45I GTSAC 2 cut(s) 2629, 2756
TspRI CASTG 2 cut(s) 1558, 2409
Van91I CCANNNNNTGG 2 cut(s) 133, 1250
Vha464I CTTAAG 4 cut(s) 1274, 1762, 1910, 2273
VpaK11BI GGWCC 2 cut(s) 498, 1467
XapI RAATTY 6 cut(s) 433, 667, 776, 1352, 1819, 2158
XceI RCATGY 6 cut(s) 283, 1610, 1712, 2545, 2587, 2739
XcmI CCANNNNNNNNNTGG 2 cut(s) 566, 601
XmiI GTMKAC 1 cut(s) 69
XmnI GAANNNNTTC 2 cut(s) 580, 2844
XspI CTAG 4 cut(s) 23, 915, 1088, 1602
ZraI GACGTC 1 cut(s) 2861
ZrmI AGTACT 3 cut(s) 27, 481, 1445
Zsp2I ATGCAT 3 cut(s) 1515, 1714, 2463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.