Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001339

Physical Location & Seq

Genomic Coordinates Reverse (-)

4650109 .. 4657177

Gene Structure 7069 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1049100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1632 bp

ATGGTGCTCGCTGTCGCTATTGCCACTCTGGTAGTTTCAACCGGCATCACATCCTCACTTTTTACTGTTTTGTACAACAAACTTGCAGATCTCACTTTGGAGAAGATAGGCAAGAATGATGACGTCGCCGACAAGCTCCAAAAGCTCGCACGAACGCTGCTGAAAATCGAGTCTCTTGTCGACGACATGGAGAACAAGCAAACCAACTCCAACTTCTGCGAGATGCTCATTGAACATGTCAAGAGTGCCCTTTTCGACGCTGAAGATCTGCTGGATAAGATCGATTTCGGAAAGCTGGAATTCAAAACCGCCATATTGAGTGTGAAGTCGGAAGGGGAGGTTGCGGTCATTCGGATGCTGGAAAGAGTTGAAGATTTAGTGAAAGAGATGGAGGGTTACCTCTTGTCGGAAATTAACAAGAAGCGCAGTCCTCCTCAGAGTTTGGATGTTAATCCTCGCACTAGTTCGTTGGTTGACAAGCTGTCCGTGTTCGGCAGGGATAACGAGAAGGAGAAGATGGTTGAGATGCTGCTGGCGCAGCGGACGGAGAATGTTTCTGTGATTCCGATTTGGGGTATGGGCGGGACCGGAAAAACAACACTTGCTCAACTTGTGTACAACGATCAAGCTGTCGTTCGAAATTTTAAGTTGAGGATGTGGGTGTGTTTTTCTTCTCTAGACACTGACCTGGTTACCGTCACTAAATCAATTTATGAGTCTGCTACCAACGAGGTTTTGGAGTTGCCAAATTTGGATGCCGTTCAGGTCGCACTGCAGGAGAAAATGCGGAGGAAGAAGTTTTTGCTCGTACTCGACGGAATGTGGAATGAGAATCCGGCTTTCTGGGATGCGTTGAAATTGCCCTTCAGATTTGCGGCCCCAGGGAGCAACATTGTTGTAACTACTCGAAGCAAAATTGTTTCGTTGATTGTAAGCACTGGTCAGCGGTATGATTTGCACACTTTGTCCGGTGACTCGGGTGATGAAGCTTGTCGGGACATAATTAAACAGAGCTTCGGTGGGAATCTCGATGGACATGGAGATTTATGGAAGATAGCAAAAAAGTGTAAAGGCCTCCCGCTGGCAGCCAAAGTGATCGGACGTGTCTTGCGATTCAAGTTAGGAACGAAGGAGTGGGATCACATTCTAAATTATGAGCTTTGGGACTTTCCAGAAGATTACAAGAGCCAAATCTACCCTGTACTTAAAGTGAGCTATGATCACTTGCCGTCGAATTTGAAAAGATGTTTTGGTTATTGTTCGATTTTCCCTCACAAGTTTCAATTTGAAGAGGATGATTTAATCCAACTATGGGCAGCAGAAGGCTTCATCCAACCCCAAAGAACAAGAAGTGTCCAAGGCATTGGAAGAGAGTACATTGATTTCTTGCTCTCGAGGTCTCTTTTCCAAGTTTCACCAAACGATGCAGACCGCCAAACAGAGGCAGAGCCACATGGAGGACAGGTCCCTCCTGAGAGTGATTTATGGCCCCGCCACTGCCGCCGAAAATATGAAATCCATGGCTTCATTCATGATATGGCGAGACTGGTTTCGGCCAACTTATGCTTTCGTCTGGAGGATAGCATGTCAACCTTCTTGCCCTCTAAAAATGTGGAACACGAAGCCCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_8 NB-ARC

Protein Analysis

544

Amino Acids

61.62

Weight (kDa)

5.88

Isoelectric Point (pI)

38.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rx_N	PF18052	23 - 96	1.1e-10	Rx N-terminal domain
NB-ARC	PF00931	168 - 319	1.4e-24	NB-ARC domain
WHD_DRP	PF23559	422 - 475	2.6e-16	Disease resistance protein Winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001339)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g25750 FvH4_4g25750 FvH4_4g25750 FvH4_4g25750 FvH4_5g16110 FvH4_5g16110 FvH4_5g16110 FvH4_5g16110 FvH4_5g16110 FvH4_5g34680 FvH4_5g34680 FvH4_5g34680
malus_domestica	MD14G1049100.v1.1
pyrus_communis	pycom14g04040
rosa_chinensis	RchiOBHm_Chr4g0433191 RchiOBHm_Chr7g0235331
rosa_laevigata	RLG00000006775
rosa_multiflora	Rmu_co8059592.1_g000001 Rmu_sc0000957.1_g000011 Rmu_sc0000962.1_g000009 Rmu_sc0002882.1_g000047 Rmu_sc0004637.1_g000016 Rmu_sc0004657.1_g000071 Rmu_sc0031691.1_g000001
rosa_roxburghii	Rroxscaffold_1G00028630 Rroxscaffold_2G00103020 Rroxscaffold_2G00103050 Rroxscaffold_3G00226110 Rroxscaffold_5G00346070 Rroxscaffold_5G00346080
rosa_rugosa	Rorug05G0474500 Rorug05G0474600 Rorug05G0474600 Rorug05G0474600 Rorug07G0132300 Rorug07G0289900
rosa_samantha	Rh3DG202500 Rh4AG320400 Rh4AG320600 Rh4AG320800 Rh4DG324000 Rh4DG324400 Rh4DG324500 Rh4DG324800 Rh7AG265300 Rh7AG444900 Rh7CG282100 Rh7CG465400 Rh7CG465500 Rh7DG433700
rosa_wichuraiana	Rw0G008720 Rw4G027820 Rw7G022720 Rw7G036950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	126
AccI	GTMKAC	1 cut(s)	180
AclWI	GGATC	1 cut(s)	1146
AcoI	YGGCCR	1 cut(s)	1554
AcsI	RAATTY	4 cut(s)	299, 640, 748, 1234
AcuI	CTGAAG	2 cut(s)	282, 850
AcyI	GRCGYC	1 cut(s)	123
AfaI	GTAC	5 cut(s)	74, 617, 810, 1203, 1376
AfiI	CCNNNNNNNGG	6 cut(s)	406, 572, 1081, 1312, 1441, 1457
AflIII	ACRYGT	2 cut(s)	235, 1102
AgsI	TTSAA	9 cut(s)	39, 233, 304, 371, 856, 1117, 1240, 1283, 1289
AhdI	GACNNNNNGTC	1 cut(s)	481
AhlI	ACTAGT	1 cut(s)	461
AjiI	CACGTC	1 cut(s)	1103
AjnI	CCWGG	2 cut(s)	687, 880
AjuI	GAANNNNNNNTTGG	2 cut(s)	197, 229
AluBI	AGCT	9 cut(s)	136, 145, 295, 481, 629, 989, 1014, 1159, 1215
AluI	AGCT	9 cut(s)	136, 145, 295, 481, 629, 989, 1014, 1159, 1215
Alw21I	GWGCWC	1 cut(s)	9
Alw26I	GTCTC	3 cut(s)	177, 1404, 1537
AlwI	GGATC	1 cut(s)	1146
Ama87I	CYCGRG	2 cut(s)	976, 1393
AoxI	GGCC	4 cut(s)	876, 1072, 1487, 1554
ApeKI	GCWGC	5 cut(s)	157, 529, 538, 1085, 1316
ApoI	RAATTY	4 cut(s)	299, 640, 748, 1234
AspLEI	GCGC	2 cut(s)	426, 538
AspS9I	GGNCC	4 cut(s)	585, 877, 1465, 1488
AsuHPI	GGTGA	3 cut(s)	983, 992, 1407
AsuII	TTCGAA	1 cut(s)	637
AvaI	CYCGRG	2 cut(s)	976, 1393
AvaII	GGWCC	2 cut(s)	585, 1465
BaeGI	GKGCMC	1 cut(s)	250
Bbv12I	GWGCWC	1 cut(s)	9
BbvI	GCAGC	5 cut(s)	144, 516, 550, 1097, 1328
BccI	CCATC	3 cut(s)	382, 511, 1025
BceAI	ACGGC	2 cut(s)	743, 1213
BciT130I	CCWGG	2 cut(s)	689, 882
BclI	TGATCA	1 cut(s)	1219
BcoDI	GTCTC	3 cut(s)	177, 1404, 1537
BcuI	ACTAGT	1 cut(s)	461
BfaI	CTAG	3 cut(s)	462, 677, 1630
BfmI	CTRYAG	1 cut(s)	773
BglII	AGATCT	2 cut(s)	88, 265
BisI	GCNGC	7 cut(s)	158, 530, 539, 876, 1086, 1317, 1501
BlsI	GCNGC	7 cut(s)	159, 531, 540, 877, 1087, 1318, 1502
Bme1390I	CCNGG	2 cut(s)	689, 882
Bme18I	GGWCC	2 cut(s)	585, 1465
BmeRI	GACNNNNNGTC	1 cut(s)	481
BmeT110I	CYCGRG	2 cut(s)	976, 1393
BmgBI	CACGTC	1 cut(s)	1103
BmgT120I	GGNCC	4 cut(s)	585, 877, 1465, 1488
BmiI	GGNNCC	4 cut(s)	586, 879, 1467, 1490
BmrFI	CCNGG	2 cut(s)	689, 882
BmsI	GCATC	7 cut(s)	54, 213, 345, 516, 745, 838, 1414
BpmI	CTGGAG	1 cut(s)	1595
Bpu14I	TTCGAA	1 cut(s)	637
Bsa29I	ATCGAT	1 cut(s)	282
BsaBI	GATNNNNATC	1 cut(s)	450
BsaHI	GRCGYC	1 cut(s)	123
BsaI	GGTCTC	1 cut(s)	1404
BsaJI	CCNNGG	4 cut(s)	880, 881, 1357, 1519
BsaWI	WCCGGW	2 cut(s)	587, 968
BsaXI	ACNNNNNCTCC	2 cut(s)	1449, 1479
Bsc4I	CCNNNNNNNGG	6 cut(s)	406, 572, 1081, 1312, 1441, 1457
Bse118I	RCCGGY	1 cut(s)	41
Bse1I	ACTGG	2 cut(s)	943, 1551
Bse8I	GATNNNNATC	1 cut(s)	450
BseBI	CCWGG	2 cut(s)	689, 882
BseCI	ATCGAT	1 cut(s)	282
BseDI	CCNNGG	4 cut(s)	880, 881, 1357, 1519
BseGI	GGATG	8 cut(s)	50, 360, 451, 660, 760, 853, 1300, 1329
BseJI	GATNNNNATC	1 cut(s)	450
BseLI	CCNNNNNNNGG	6 cut(s)	406, 572, 1081, 1312, 1441, 1457
BseMII	CTCAG	2 cut(s)	449, 1464
BseNI	ACTGG	2 cut(s)	943, 1551
BseRI	GAGGAG	1 cut(s)	423
BseSI	GKGCMC	1 cut(s)	250
BseXI	GCAGC	5 cut(s)	144, 516, 550, 1097, 1328
BshFI	GGCC	4 cut(s)	878, 1074, 1489, 1556
BshVI	ATCGAT	1 cut(s)	282
BsiHKAI	GWGCWC	1 cut(s)	9
BsiHKCI	CYCGRG	2 cut(s)	976, 1393
BsiSI	CCGG	4 cut(s)	42, 588, 836, 969
BslFI	GGGAC	4 cut(s)	598, 1010, 1178, 1451
BslI	CCNNNNNNNGG	6 cut(s)	406, 572, 1081, 1312, 1441, 1457
BsmAI	GTCTC	3 cut(s)	177, 1404, 1537
BsmFI	GGGAC	4 cut(s)	598, 1010, 1178, 1451
BsnI	GGCC	4 cut(s)	878, 1074, 1489, 1556
Bso31I	GGTCTC	1 cut(s)	1404
BsoBI	CYCGRG	2 cut(s)	976, 1393
Bsp119I	TTCGAA	1 cut(s)	637
Bsp1286I	GDGCHC	2 cut(s)	9, 250
Bsp1407I	TGTACA	2 cut(s)	72, 615
Bsp143I	GATC	7 cut(s)	88, 265, 279, 622, 1095, 1138, 1219
Bsp19I	CCATGG	1 cut(s)	1519
BspANI	GGCC	4 cut(s)	878, 1074, 1489, 1556
BspCNI	CTCAG	2 cut(s)	448, 1465
BspDI	ATCGAT	1 cut(s)	282
BspHI	TCATGA	1 cut(s)	1531
BspLI	GGNNCC	4 cut(s)	586, 879, 1467, 1490
BspMAI	CTGCAG	1 cut(s)	777
BspPI	GGATC	1 cut(s)	1146
BspT104I	TTCGAA	1 cut(s)	637
BspTNI	GGTCTC	1 cut(s)	1404
BsrFI	RCCGGY	1 cut(s)	41
BsrGI	TGTACA	2 cut(s)	72, 615
BsrI	ACTGG	2 cut(s)	943, 1551
BssAI	RCCGGY	1 cut(s)	41
BssECI	CCNNGG	4 cut(s)	880, 881, 1357, 1519
BssMI	GATC	7 cut(s)	88, 265, 279, 622, 1095, 1138, 1219
BssNI	GRCGYC	1 cut(s)	123
BssT1I	CCWWGG	2 cut(s)	1357, 1519
Bst2UI	CCWGG	2 cut(s)	689, 882
Bst4CI	ACNGT	2 cut(s)	67, 697
Bst6I	CTCTTC	2 cut(s)	1284, 1363
BstACI	GRCGYC	1 cut(s)	123
BstAUI	TGTACA	2 cut(s)	72, 615
BstBI	TTCGAA	1 cut(s)	637
BstC8I	GCNNGC	4 cut(s)	9, 147, 534, 1083
BstDEI	CTNAG	2 cut(s)	435, 1473
BstDSI	CCRYGG	1 cut(s)	1519
BstEII	GGTNACC	2 cut(s)	395, 691
BstF5I	GGATG	8 cut(s)	50, 360, 451, 660, 760, 853, 1300, 1329
BstHHI	GCGC	2 cut(s)	426, 538
BstKTI	GATC	7 cut(s)	91, 268, 282, 625, 1098, 1141, 1222
BstMAI	GTCTC	3 cut(s)	177, 1404, 1537
BstMBI	GATC	7 cut(s)	88, 265, 279, 622, 1095, 1138, 1219
BstMWI	GCNNNNNNNGC	4 cut(s)	142, 535, 538, 1500
BstNI	CCWGG	2 cut(s)	689, 882
BstNSI	RCATGY	2 cut(s)	239, 1588
BstPI	GGTNACC	2 cut(s)	395, 691
BstSCI	CCNGG	2 cut(s)	687, 880
BstSFI	CTRYAG	1 cut(s)	773
BstSLI	GKGCMC	1 cut(s)	250
BstV1I	GCAGC	5 cut(s)	144, 516, 550, 1097, 1328
BstX2I	RGATCY	2 cut(s)	88, 265
BstXI	CCANNNNNNTGG	1 cut(s)	1364
BstYI	RGATCY	2 cut(s)	88, 265
Bsu15I	ATCGAT	1 cut(s)	282
BsuRI	GGCC	4 cut(s)	878, 1074, 1489, 1556
BsuTUI	ATCGAT	1 cut(s)	282
BtgI	CCRYGG	1 cut(s)	1519
BtrI	CACGTC	1 cut(s)	1103
BtsCI	GGATG	8 cut(s)	50, 360, 451, 660, 760, 853, 1300, 1329
BtsI	GCAGTG	2 cut(s)	770, 1495
BtsIMutI	CAGTG	4 cut(s)	681, 770, 936, 1495
Cac8I	GCNNGC	4 cut(s)	9, 147, 534, 1083
CciI	TCATGA	1 cut(s)	1531
CfoI	GCGC	2 cut(s)	426, 538
Cfr10I	RCCGGY	1 cut(s)	41
Cfr13I	GGNCC	4 cut(s)	585, 877, 1465, 1488
ClaI	ATCGAT	1 cut(s)	282
CseI	GACGC	1 cut(s)	266
CsiI	ACCWGGT	1 cut(s)	687
Csp6I	GTAC	5 cut(s)	73, 616, 809, 1202, 1375
CviAII	CATG	7 cut(s)	187, 236, 1037, 1454, 1520, 1532, 1585
CviQI	GTAC	5 cut(s)	73, 616, 809, 1202, 1375
DdeI	CTNAG	2 cut(s)	435, 1473
DpnI	GATC	7 cut(s)	90, 267, 281, 624, 1097, 1140, 1221
DpnII	GATC	7 cut(s)	88, 265, 279, 622, 1095, 1138, 1219
DriI	GACNNNNNGTC	1 cut(s)	481
EaeI	YGGCCR	1 cut(s)	1554
Eam1104I	CTCTTC	2 cut(s)	1284, 1363
Eam1105I	GACNNNNNGTC	1 cut(s)	481
EarI	CTCTTC	2 cut(s)	1284, 1363
Eco130I	CCWWGG	2 cut(s)	1357, 1519
Eco147I	AGGCCT	1 cut(s)	1074
Eco31I	GGTCTC	1 cut(s)	1404
Eco47I	GGWCC	2 cut(s)	585, 1465
Eco57I	CTGAAG	2 cut(s)	282, 850
Eco88I	CYCGRG	2 cut(s)	976, 1393
Eco91I	GGTNACC	2 cut(s)	395, 691
EcoO109I	RGGNCCY	1 cut(s)	1465
EcoO65I	GGTNACC	2 cut(s)	395, 691
EcoRI	GAATTC	1 cut(s)	299
EcoRII	CCWGG	2 cut(s)	687, 880
EcoT14I	CCWWGG	2 cut(s)	1357, 1519
ErhI	CCWWGG	2 cut(s)	1357, 1519
FaeI	CATG	7 cut(s)	190, 239, 1040, 1457, 1523, 1535, 1588
FaqI	GGGAC	4 cut(s)	598, 1010, 1178, 1451
FatI	CATG	7 cut(s)	186, 235, 1036, 1453, 1519, 1531, 1584
FauI	CCCGC	3 cut(s)	575, 1086, 1499
FbaI	TGATCA	1 cut(s)	1219
FblI	GTMKAC	1 cut(s)	180
Fnu4HI	GCNGC	7 cut(s)	158, 530, 539, 876, 1086, 1317, 1501
FokI	GGATG	8 cut(s)	37, 367, 458, 667, 767, 860, 1307, 1316
Fsp4HI	GCNGC	7 cut(s)	158, 530, 539, 876, 1086, 1317, 1501
FspBI	CTAG	3 cut(s)	462, 677, 1630
GlaI	GCGC	2 cut(s)	425, 537
GluI	GCNGC	7 cut(s)	158, 530, 539, 876, 1086, 1317, 1501
GsuI	CTGGAG	1 cut(s)	1595
HaeIII	GGCC	4 cut(s)	878, 1074, 1489, 1556
HapII	CCGG	4 cut(s)	42, 588, 836, 969
HgaI	GACGC	1 cut(s)	266
HhaI	GCGC	2 cut(s)	426, 538
Hin1I	GRCGYC	1 cut(s)	123
Hin1II	CATG	7 cut(s)	190, 239, 1040, 1457, 1523, 1535, 1588
Hin6I	GCGC	2 cut(s)	424, 536
HinP1I	GCGC	2 cut(s)	424, 536
HincII	GTYRAC	3 cut(s)	181, 475, 1590
HindII	GTYRAC	3 cut(s)	181, 475, 1590
HindIII	AAGCTT	1 cut(s)	987
HinfI	GANTC	7 cut(s)	170, 562, 716, 832, 974, 1024, 1113
HpaII	CCGG	4 cut(s)	42, 588, 836, 969
HphI	GGTGA	3 cut(s)	983, 992, 1407
Hpy166II	GTNNAC	4 cut(s)	181, 475, 616, 1590
Hpy188I	TCNGA	8 cut(s)	290, 331, 354, 409, 438, 567, 869, 1100
Hpy188III	TCNNGA	9 cut(s)	241, 677, 995, 1028, 1172, 1393, 1472, 1532, 1574
Hpy8I	GTNNAC	4 cut(s)	181, 475, 616, 1590
Hpy99I	CGWCG	5 cut(s)	128, 185, 260, 818, 1234
HpyAV	CCTTC	6 cut(s)	326, 502, 874, 1123, 1316, 1603
HpyCH4III	ACNGT	2 cut(s)	67, 697
HpyCH4IV	ACGT	2 cut(s)	123, 1102
HpyCH4V	TGCA	4 cut(s)	86, 775, 958, 1427
HpyF10VI	GCNNNNNNNGC	4 cut(s)	142, 535, 538, 1500
HpyF3I	CTNAG	2 cut(s)	435, 1473
HpySE526I	ACGT	2 cut(s)	123, 1102
Hsp92I	GRCGYC	1 cut(s)	123
Hsp92II	CATG	7 cut(s)	190, 239, 1040, 1457, 1523, 1535, 1588
HspAI	GCGC	2 cut(s)	424, 536
Ksp22I	TGATCA	1 cut(s)	1219
Kzo9I	GATC	7 cut(s)	88, 265, 279, 622, 1095, 1138, 1219
LmnI	GCTCC	2 cut(s)	141, 885
Lsp1109I	GCAGC	5 cut(s)	144, 516, 550, 1097, 1328
LweI	GCATC	7 cut(s)	54, 213, 345, 516, 745, 838, 1414
MabI	ACCWGGT	1 cut(s)	687
MaeI	CTAG	3 cut(s)	462, 677, 1630
MaeII	ACGT	2 cut(s)	123, 1102
MaeIII	GTNAC	5 cut(s)	395, 691, 697, 898, 971
MalI	GATC	7 cut(s)	90, 267, 281, 624, 1097, 1140, 1221
MboI	GATC	7 cut(s)	88, 265, 279, 622, 1095, 1138, 1219
MflI	RGATCY	2 cut(s)	88, 265
MhlI	GDGCHC	2 cut(s)	9, 250
MlyI	GAGTC	3 cut(s)	179, 725, 968
MmeI	TCCRAC	5 cut(s)	234, 309, 387, 1330, 1357
MseI	TTAA	6 cut(s)	414, 450, 645, 1005, 1206, 1301
MslI	CAYNNNNRTG	1 cut(s)	353
MspA1I	CMGCKG	3 cut(s)	541, 946, 1081
MspI	CCGG	4 cut(s)	42, 588, 836, 969
MspR9I	CCNGG	2 cut(s)	689, 882
MvaI	CCWGG	2 cut(s)	689, 882
MwoI	GCNNNNNNNGC	4 cut(s)	142, 535, 538, 1500
NcoI	CCATGG	1 cut(s)	1519
NdeII	GATC	7 cut(s)	88, 265, 279, 622, 1095, 1138, 1219
NlaIII	CATG	7 cut(s)	190, 239, 1040, 1457, 1523, 1535, 1588
NlaIV	GGNNCC	4 cut(s)	586, 879, 1467, 1490
NmuCI	GTSAC	2 cut(s)	697, 971
NspI	RCATGY	2 cut(s)	239, 1588
NspV	TTCGAA	1 cut(s)	637
PaeR7I	CTCGAG	1 cut(s)	1393
PagI	TCATGA	1 cut(s)	1531
PasI	CCCWGGG	1 cut(s)	881
PceI	AGGCCT	1 cut(s)	1074
PciI	ACATGT	1 cut(s)	235
PcsI	WCGNNNNNNNCGW	1 cut(s)	1108
PfeI	GAWTC	4 cut(s)	562, 832, 1024, 1113
PkrI	GCNGC	7 cut(s)	159, 531, 540, 877, 1087, 1318, 1502
PleI	GAGTC	3 cut(s)	178, 724, 968
PpsI	GAGTC	3 cut(s)	178, 724, 968
PpuMI	RGGWCCY	1 cut(s)	1465
PscI	ACATGT	1 cut(s)	235
Psp5II	RGGWCCY	1 cut(s)	1465
Psp6I	CCWGG	2 cut(s)	687, 880
PspEI	GGTNACC	2 cut(s)	395, 691
PspGI	CCWGG	2 cut(s)	687, 880
PspN4I	GGNNCC	4 cut(s)	586, 879, 1467, 1490
PspPI	GGNCC	4 cut(s)	585, 877, 1465, 1488
PspPPI	RGGWCCY	1 cut(s)	1465
PstI	CTGCAG	1 cut(s)	777
PsuI	RGATCY	2 cut(s)	88, 265
RsaI	GTAC	5 cut(s)	74, 617, 810, 1203, 1376
RsaNI	GTAC	5 cut(s)	73, 616, 809, 1202, 1375
RseI	CAYNNNNRTG	1 cut(s)	353
SalI	GTCGAC	1 cut(s)	179
SaqAI	TTAA	6 cut(s)	414, 450, 645, 1005, 1206, 1301
SatI	GCNGC	7 cut(s)	158, 530, 539, 876, 1086, 1317, 1501
Sau3AI	GATC	7 cut(s)	88, 265, 279, 622, 1095, 1138, 1219
Sau96I	GGNCC	4 cut(s)	585, 877, 1465, 1488
SchI	GAGTC	3 cut(s)	179, 725, 968
ScrFI	CCNGG	2 cut(s)	689, 882
SduI	GDGCHC	2 cut(s)	9, 250
SexAI	ACCWGGT	1 cut(s)	687
SfaNI	GCATC	7 cut(s)	54, 213, 345, 516, 745, 838, 1414
SfcI	CTRYAG	1 cut(s)	773
Sfr274I	CTCGAG	1 cut(s)	1393
SfuI	TTCGAA	1 cut(s)	637
SinI	GGWCC	2 cut(s)	585, 1465
SlaI	CTCGAG	1 cut(s)	1393
SmiMI	CAYNNNNRTG	1 cut(s)	353
SmlI	CTYRAG	1 cut(s)	1393
SmoI	CTYRAG	1 cut(s)	1393
SpeI	ACTAGT	1 cut(s)	461
SseBI	AGGCCT	1 cut(s)	1074
SspMI	CTAG	3 cut(s)	462, 677, 1630
StuI	AGGCCT	1 cut(s)	1074
StyD4I	CCNGG	2 cut(s)	687, 880
StyI	CCWWGG	2 cut(s)	1357, 1519
TaaI	ACNGT	2 cut(s)	67, 697
TaiI	ACGT	2 cut(s)	126, 1105
TatI	WGTACW	4 cut(s)	72, 615, 1201, 1374
TauI	GCSGC	2 cut(s)	878, 1503
TfiI	GAWTC	4 cut(s)	562, 832, 1024, 1113
Tru1I	TTAA	6 cut(s)	414, 450, 645, 1005, 1206, 1301
Tru9I	TTAA	6 cut(s)	414, 450, 645, 1005, 1206, 1301
TscAI	CASTG	4 cut(s)	688, 777, 943, 1502
TseFI	GTSAC	2 cut(s)	697, 971
TseI	GCWGC	5 cut(s)	157, 529, 538, 1085, 1316
Tsp45I	GTSAC	2 cut(s)	697, 971
TspDTI	ATGAA	5 cut(s)	999, 1318, 1516, 1520, 1527
TspGWI	ACGGA	3 cut(s)	475, 560, 831
TspRI	CASTG	4 cut(s)	688, 777, 943, 1502
VpaK11BI	GGWCC	2 cut(s)	585, 1465
XapI	RAATTY	4 cut(s)	299, 640, 748, 1234
XbaI	TCTAGA	1 cut(s)	676
XceI	RCATGY	2 cut(s)	239, 1588
XcmI	CCANNNNNNNNNTGG	1 cut(s)	733
XhoI	CTCGAG	1 cut(s)	1393
XmiI	GTMKAC	1 cut(s)	180
XspI	CTAG	3 cut(s)	462, 677, 1630
ZraI	GACGTC	1 cut(s)	124

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.