Rh4AG320400

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001339
Physical Location & Seq
Reverse (-)
63301128 .. 63306432
5305 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG320400.1

Sequence Viewer

Length: 2976 bp
ATGGCAGAGATAGCATCCCTGGTACTCTCGACCGGCATTACGTCGGCAATTCTTCAGGTTGTTGTTGACAGAGTCACAAAATTTGTTGAACAACAGATAGGCTTGTTCACTGGGGTCAACGGCAGCGTCAGAGAGTTGGAGAGAACGCTGATGGAGATTCAAGCTCGTGTTGATGTTATAGAAAAGAAATATTGCATTACGAAGGCAGCAGAAGTTTTGCTCGAAGATCTCCTGACTAGTTTTTTGGATGCTGAAGATTTGCTGGACAAGATCGGTTTGCGTGAGGAGGTCAACAGTGGGAGTCAGGTACGTCACTCGATGCCCTTCTATTCTGGCATACCGTCTGAGATTAAAAAGATGCAAGAGAAACTGGATGGGTTGATAAAAGACCTGGAAAGGTACTCTACCACAGAAATCATCCAGTTGAGAGAGCCTAAAGAGAAGGCTATTGCTCCTCCTACTTCTTTGGTTGATGAATCTTGTGTATTTGGAAGGGATACAGATAAGAACAAGATGAAAAAAATGGTCTTGTCTGTGCAACAAGAGGGGGGAAATGTTTCTGTAATTCCAATAGTAGGGATGGCTGGGATTGGGAAAACAACACTTGCCCAACTCGTCTACAATGATGCAGATGTGCATAAGAAATTTGATTTGAAAATGTGGGTCTCTGTTTCTTTAGACCATGGTCTGTGCATGATTACAGAATCTATTCTTGAGTCCGCTACTGGAGGAAAGGGTTCTCAGTTGTCAAGCTTGGATAGTGTTCAGGTTGAATTACACAAGACATTGAAGGGGAAGAGGTTTTTACTTGTACTAGATGGCATTTCTAATGATAGTCTTAGTGATTGGGATCAATTCAGGTTGTCTTTTGCGGCTGCAAAGAAGGGGAGCAAGATTGTGATGACTACTCGAAGTGAAATTTCTGCCTCAATTGTTGCCTCTACATCATATCGTTTGGATCCTCTGTGTGACAATGCTTGTTGGGAAATAATCAAACAAAGAGGGAATCTGAATGGGCAGACTAATTTGGAGGCGAATGGCCTAGCAATTTCGAAGAAGTGCAGAGGGTTTCCATGGGTAGCAAAAGTGATTGGAAGCTGTTTGAATTCGGGAGAGATGGAGTGGGATACTCTGTTAAAGTATGAGCTTTGGGAACTGCAGGAACATAAAACACGAATCTATCCAGTCCTCAAATTGTTGAGCTATGATAATATGCCTTCTTATTTGAAAAGATGTTTCAGTTATTGTTCTATCTTTCCTCGTGATCATGTAGTTGAGAAGGATGATTTGGTCCAACTATGGGCAGCAGAAGGCTTTATTCAGTCCCAAGGAACAGGAAAAATTGAAGATGTTGGAAGACAGTATTTTGAGGACTTGCATTCGAGGGCTTTCTTTTCACAAGTTCCAAATCAGCAAAGATATAAAATGCATGAGCTCATTCATGATTTGGCCAGATTTGTTTCAACCAACTTATGCTTTTGTATGGAGGACAATGTGCCATGCTTGGTGCAAGTATCTCCTAATGCTCGGCATGCGTCTTTGCTTTATGAAAACGCTCAACTATCTACATTGGAGGTGTTTTACAAGTATAAGAAGTTGAGGACATTTACATTGCTTCCTAAATTCCCATCTATTCCGAGGCCAGTCATGTTGCTTCCCAATTTTTCGTCCAATCTTGGCGAAGTCCCTCATGAATTTTTTGAAAGGTTGGGATGCTTGAGGGTGTTGAATTTGAGTCATAGTGCCATCTCTAAATTGCCTGAATCTATTGGAAATTTGATTCATCTTCGTTACCTCAATCTTAGTCATACCCGTATTGAACTATTGCCAAAGCCTATAACAACGATATATGGGTTAGAAACATTGAAGCTCAAGAGTTGTCCTAAGCTTCTTCATTTGCCTGAGAACTTAAAGGACTTGATTAAGCTAAGACATCTTGACTTTGACAGACACCGTCAACTAAGTTCCATGCCAATAGATGTTGGGAAGTTAACTAGTCTTGAAACCCTTCATGCATTTAAAGTGGGAAAAGAGAAAGGATATCAGATTGAGGAGCTTAAGAACATGAGGTGTCTTCGTGGATCCATTTTTATTACGAATCTTGAGAATGTGGCAGATTCTATGCAGGCTGAGGCTGCTATGTTACACAACAAGCAATACTTAGACAGGTTGGAGCTGGAATGGAATGGAATCAGAGGTCAAATGCAACCAGTTCAGCAAGAAGTTCTCACTGGGCTTGAACCTCATGCTGGGTTAAAGGAGTTACGAGTAGCAGGTTACTGTGGTTTGTTATTTCCCAGATGGATAGCCAACTCAGCATTTAGCAAGCTTGAGGGTATTGAATTAGAAAATTGCCCATTTTGTGAACTGCTTCCTTCTCTTGGCCAACTTCCGGCTCTCAAGAAACTTCTTATTCAAAATATGTCAACTTTGGAAGGTGTGGATCGTCTGTTTTGTGGTTCCTCAGCTGCTTTTCTATCCTTGGAGATGCTAACACTTCGTGACATGCCGGAGTTGAGTAACTGGTCAGGGCTACGTGACAATGACATGCCTTGCCTCAAAGACCTCAGCATTGATTCTTGTCCAAAGTTGGCCACCCTACCTTCATTTCATTACCTCACTTCACTTCAATGTTTAGACATCAATCGTTGCCCTCAGCTTGAATCTTTGCCAGAGGGAGGACTGCCACCCTCACTCAAAACTTTGATCATTTTGGAGAGTGACATCTTGAAGGATCGGTGCAAGGTGGGAGAAGGTGCAGATTGGAATACGATCAGATGGATCCCTACCATATTGATTGAAAATATGGTGATTCCAGCGCAAAGACCGGATAACCAGATTGATGAGGTTCAGAGCGGTCCAGAAAACATGTTTAGTAGCAGAAACTCCTTGTTGATTTGTTCTGTTATTCTTTTTCCTCTGCTCTTCCTTTTTATGAGATCATTGTACCAGTACTTGTCCCAAGCCAGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

991

Amino Acids

111.85

Weight (kDa)

6.22

Isoelectric Point (pI)

41.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 18 - 98 1e-08 Rx N-terminal domain
NB-ARC PF00931 172 - 335 3.8e-29 NB-ARC domain
WHD_DRP PF23559 418 - 484 5.2e-24 Disease resistance protein Winged helix domain
LRR_8 PF13855 556 - 606 6.1e-08 Leucine rich repeat
LRR_14 PF23598 569 - 860 2.3e-28 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 683 - 808 7.7e-37 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2264
AccBSI CCGCTC 1 cut(s) 2856
AccI GTMKAC 1 cut(s) 618
AciI CCGC 3 cut(s) 720, 872, 2856
AclWI GGATC 9 cut(s) 858, 953, 966, 2076, 2089, 2451, 2742, 2776, 2789
AcoI YGGCCR 3 cut(s) 1449, 2383, 2592
AcsI RAATTY 8 cut(s) 80, 644, 918, 1105, 1622, 1694, 1729, 1774
AcuI CTGAAG 2 cut(s) 38, 273
AdeI CACNNNGTG 1 cut(s) 2501
AfaI GTAC 6 cut(s) 24, 309, 401, 813, 2948, 2954
AfiI CCNNNNNNNGG 6 cut(s) 575, 1300, 2249, 2381, 2392, 2678
AflII CTTAAG 1 cut(s) 2057
AflIII ACRYGT 1 cut(s) 2868
AhlI ACTAGT 2 cut(s) 236, 1994
AjnI CCWGG 2 cut(s) 18, 390
AjuI GAANNNNNNNTTGG 2 cut(s) 1271, 1303
Alw21I GWGCWC 1 cut(s) 1437
Alw26I GTCTC 1 cut(s) 670
AlwI GGATC 9 cut(s) 858, 953, 966, 2076, 2089, 2451, 2742, 2776, 2789
AoxI GGCC 5 cut(s) 1039, 1449, 1640, 2383, 2592
ApeKI GCWGC 6 cut(s) 123, 206, 875, 1304, 2135, 2468
ApoI RAATTY 8 cut(s) 80, 644, 918, 1105, 1622, 1694, 1729, 1774
ArsI GACNNNNNNTTYG 2 cut(s) 226, 258
Asp700I GAANNNNTTC 5 cut(s) 556, 708, 736, 2007, 2370
AspLEI GCGC 1 cut(s) 2821
AspS9I GGNCC 2 cut(s) 1291, 2858
AsuHPI GGTGA 1 cut(s) 2821
AsuII TTCGAA 1 cut(s) 1052
AvaII GGWCC 2 cut(s) 1291, 2858
BalI TGGCCA 3 cut(s) 1451, 2385, 2594
BamHI GGATCC 3 cut(s) 958, 2081, 2781
BanII GRGCYC 1 cut(s) 1437
BauI CACGAG 2 cut(s) 165, 1260
BbsI GAAGAC 2 cut(s) 1363, 2066
Bbv12I GWGCWC 1 cut(s) 1437
BbvCI CCTCAGC 4 cut(s) 2130, 2464, 2567, 2655
BbvI GCAGC 6 cut(s) 135, 218, 862, 1316, 2122, 2455
BccI CCATC 9 cut(s) 145, 368, 574, 812, 1111, 1636, 1754, 2295, 2772
BceAI ACGGC 1 cut(s) 136
BciT130I CCWGG 2 cut(s) 20, 392
BciVI GTATCC 2 cut(s) 490, 1120
BclI TGATCA 2 cut(s) 1264, 2706
BcoDI GTCTC 1 cut(s) 670
BcuI ACTAGT 2 cut(s) 236, 1994
BfaI CTAG 4 cut(s) 237, 815, 1043, 1995
BfmI CTRYAG 1 cut(s) 1157
BfrI CTTAAG 1 cut(s) 2057
BfuAI ACCTGC 1 cut(s) 2264
BfuI GTATCC 2 cut(s) 490, 1120
BglII AGATCT 1 cut(s) 226
BisI GCNGC 7 cut(s) 124, 207, 873, 876, 1305, 2136, 2469
BlsI GCNGC 7 cut(s) 125, 208, 874, 877, 1306, 2137, 2470
BmcAI AGTACT 1 cut(s) 2954
Bme1390I CCNGG 2 cut(s) 20, 392
Bme18I GGWCC 2 cut(s) 1291, 2858
BmgT120I GGNCC 2 cut(s) 1291, 2858
BmiI GGNNCC 4 cut(s) 960, 2083, 2461, 2783
BmrFI CCNGG 2 cut(s) 20, 392
BmrI ACTGGG 2 cut(s) 120, 2241
BmsI GCATC 7 cut(s) 23, 238, 309, 348, 616, 1703, 2478
BmuI ACTGGG 2 cut(s) 120, 2241
BoxI GACNNNNGTC 1 cut(s) 684
BpiI GAAGAC 2 cut(s) 1363, 2066
BpmI CTGGAG 1 cut(s) 747
Bpu10I CCTNAGC 5 cut(s) 1884, 2130, 2464, 2567, 2655
Bpu14I TTCGAA 1 cut(s) 1052
BpuEI CTTGAG 6 cut(s) 734, 1738, 1856, 2123, 2351, 2384
BsaAI YACGTR 1 cut(s) 2537
BsaBI GATNNNNATC 1 cut(s) 849
BsaI GGTCTC 1 cut(s) 670
BsaJI CCNNGG 6 cut(s) 18, 682, 1073, 1327, 1637, 2481
BsaWI WCCGGW 1 cut(s) 2827
Bsc4I CCNNNNNNNGG 6 cut(s) 575, 1300, 2249, 2381, 2392, 2678
Bse118I RCCGGY 1 cut(s) 32
Bse3DI GCAATG 1 cut(s) 1610
Bse8I GATNNNNATC 1 cut(s) 849
BseBI CCWGG 2 cut(s) 20, 392
BseDI CCNNGG 6 cut(s) 18, 682, 1073, 1327, 1637, 2481
BseGI GGATG 7 cut(s) 14, 253, 379, 417, 585, 1288, 1718
BseJI GATNNNNATC 1 cut(s) 849
BseLI CCNNNNNNNGG 6 cut(s) 575, 1300, 2249, 2381, 2392, 2678
BseMI GCAATG 1 cut(s) 1610
BseMII CTCAG 8 cut(s) 336, 755, 1893, 2121, 2328, 2478, 2581, 2669
BseRI GAGGAG 3 cut(s) 299, 444, 2066
BseXI GCAGC 6 cut(s) 135, 218, 862, 1316, 2122, 2455
BseYI CCCAGC 2 cut(s) 584, 2249
BsgI GTGCAG 2 cut(s) 1081, 2778
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 5 cut(s) 1041, 1451, 1642, 2385, 2594
BsiEI CGRYCG 1 cut(s) 33
BsiHKAI GWGCWC 1 cut(s) 1437
BsiSI CCGG 4 cut(s) 33, 2393, 2510, 2828
BslFI GGGAC 3 cut(s) 1309, 1670, 2944
BslI CCNNNNNNNGG 6 cut(s) 575, 1300, 2249, 2381, 2392, 2678
BsmAI GTCTC 1 cut(s) 670
BsmFI GGGAC 3 cut(s) 1309, 1670, 2944
BsmI GAATGC 1 cut(s) 1378
BsnI GGCC 5 cut(s) 1041, 1451, 1642, 2385, 2594
Bso31I GGTCTC 1 cut(s) 670
Bsp119I TTCGAA 1 cut(s) 1052
Bsp1286I GDGCHC 1 cut(s) 1437
Bsp19I CCATGG 2 cut(s) 682, 1073
BspACI CCGC 3 cut(s) 720, 872, 2856
BspANI GGCC 5 cut(s) 1041, 1451, 1642, 2385, 2594
BspCNI CTCAG 8 cut(s) 337, 754, 1894, 2122, 2327, 2477, 2580, 2668
BspHI TCATGA 2 cut(s) 1441, 1690
BspLI GGNNCC 4 cut(s) 960, 2083, 2461, 2783
BspMAI CTGCAG 1 cut(s) 1161
BspMI ACCTGC 1 cut(s) 2264
BspPI GGATC 9 cut(s) 858, 953, 966, 2076, 2089, 2451, 2742, 2776, 2789
BspQI GCTCTTC 1 cut(s) 2930
BspT104I TTCGAA 1 cut(s) 1052
BspTI CTTAAG 1 cut(s) 2057
BspTNI GGTCTC 1 cut(s) 670
BsrBI CCGCTC 1 cut(s) 2856
BsrDI GCAATG 1 cut(s) 1610
BsrFI RCCGGY 1 cut(s) 32
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 6 cut(s) 18, 682, 1073, 1327, 1637, 2481
BssSI CACGAG 2 cut(s) 165, 1260
BssT1I CCWWGG 4 cut(s) 682, 1073, 1327, 2481
Bst2BI CACGAG 2 cut(s) 165, 1260
Bst2UI CCWGG 2 cut(s) 20, 392
Bst4CI ACNGT 5 cut(s) 296, 342, 1362, 1955, 2282
Bst6I CTCTTC 2 cut(s) 791, 2930
BstAFI CTTAAG 1 cut(s) 2057
BstBAI YACGTR 1 cut(s) 2537
BstBI TTCGAA 1 cut(s) 1052
BstC8I GCNNGC 3 cut(s) 1533, 2127, 2327
BstDSI CCRYGG 2 cut(s) 682, 1073
BstF5I GGATG 7 cut(s) 14, 253, 379, 417, 585, 1288, 1718
BstHHI GCGC 1 cut(s) 2821
BstMAI GTCTC 1 cut(s) 670
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 4 cut(s) 11, 1532, 2135, 2315
BstNI CCWGG 2 cut(s) 20, 392
BstNSI RCATGY 4 cut(s) 1535, 2509, 2551, 2872
BstPAI GACNNNNGTC 1 cut(s) 684
BstSCI CCNGG 2 cut(s) 18, 390
BstSFI CTRYAG 1 cut(s) 1157
BstV1I GCAGC 6 cut(s) 135, 218, 862, 1316, 2122, 2455
BstV2I GAAGAC 2 cut(s) 1363, 2066
BstX2I RGATCY 4 cut(s) 226, 958, 2081, 2781
BstYI RGATCY 4 cut(s) 226, 958, 2081, 2781
BsuI GTATCC 2 cut(s) 490, 1120
BsuRI GGCC 5 cut(s) 1041, 1451, 1642, 2385, 2594
BtgI CCRYGG 2 cut(s) 682, 1073
BtsCI GGATG 7 cut(s) 14, 253, 379, 417, 585, 1288, 1718
BtsIMutI CAGTG 3 cut(s) 108, 301, 2229
BveI ACCTGC 1 cut(s) 2264
Cac8I GCNNGC 3 cut(s) 1533, 2127, 2327
CciI TCATGA 2 cut(s) 1441, 1690
CfoI GCGC 1 cut(s) 2821
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 1291, 2858
CseI GACGC 2 cut(s) 115, 1524
Csp6I GTAC 6 cut(s) 23, 308, 400, 812, 2947, 2953
CviQI GTAC 6 cut(s) 23, 308, 400, 812, 2947, 2953
DraI TTTAAA 1 cut(s) 2020
DraIII CACNNNGTG 1 cut(s) 2501
EaeI YGGCCR 3 cut(s) 1449, 2383, 2592
Eam1104I CTCTTC 2 cut(s) 791, 2930
EarI CTCTTC 2 cut(s) 791, 2930
Ecl136II GAGCTC 1 cut(s) 1435
Eco130I CCWWGG 4 cut(s) 682, 1073, 1327, 2481
Eco24I GRGCYC 1 cut(s) 1437
Eco31I GGTCTC 1 cut(s) 670
Eco32I GATATC 1 cut(s) 2042
Eco47I GGWCC 2 cut(s) 1291, 2858
Eco53kI GAGCTC 1 cut(s) 1435
Eco57I CTGAAG 2 cut(s) 38, 273
EcoICRI GAGCTC 1 cut(s) 1435
EcoRI GAATTC 1 cut(s) 1105
EcoRII CCWGG 2 cut(s) 18, 390
EcoRV GATATC 1 cut(s) 2042
EcoT14I CCWWGG 4 cut(s) 682, 1073, 1327, 2481
EcoT22I ATGCAT 2 cut(s) 1431, 2017
EcoT38I GRGCYC 1 cut(s) 1437
ErhI CCWWGG 4 cut(s) 682, 1073, 1327, 2481
FalI AAGNNNNNCTT 2 cut(s) 2144, 2176
FaqI GGGAC 3 cut(s) 1309, 1670, 2944
FbaI TGATCA 2 cut(s) 1264, 2706
FblI GTMKAC 1 cut(s) 618
Fnu4HI GCNGC 7 cut(s) 124, 207, 873, 876, 1305, 2136, 2469
FokI GGATG 6 cut(s) 260, 386, 404, 592, 1295, 1725
FriOI GRGCYC 1 cut(s) 1437
Fsp4HI GCNGC 7 cut(s) 124, 207, 873, 876, 1305, 2136, 2469
FspBI CTAG 4 cut(s) 237, 815, 1043, 1995
GlaI GCGC 1 cut(s) 2820
GluI GCNGC 7 cut(s) 124, 207, 873, 876, 1305, 2136, 2469
GsaI CCCAGC 2 cut(s) 588, 2253
GsuI CTGGAG 1 cut(s) 747
HaeIII GGCC 5 cut(s) 1041, 1451, 1642, 2385, 2594
HapII CCGG 4 cut(s) 33, 2393, 2510, 2828
HgaI GACGC 2 cut(s) 115, 1524
HhaI GCGC 1 cut(s) 2821
Hin6I GCGC 1 cut(s) 2819
HinP1I GCGC 1 cut(s) 2819
HincII GTYRAC 6 cut(s) 67, 118, 292, 1958, 1992, 2427
HindII GTYRAC 6 cut(s) 67, 118, 292, 1958, 1992, 2427
HindIII AAGCTT 3 cut(s) 751, 1886, 2327
HpaI GTTAAC 1 cut(s) 1992
HpaII CCGG 4 cut(s) 33, 2393, 2510, 2828
HphI GGTGA 1 cut(s) 2821
Hpy166II GTNNAC 9 cut(s) 67, 108, 118, 292, 619, 1958, 1992, 2366, 2427
Hpy188I TCNGA 8 cut(s) 131, 346, 1011, 1638, 2046, 2195, 2777, 2853
Hpy8I GTNNAC 9 cut(s) 67, 108, 118, 292, 619, 1958, 1992, 2366, 2427
Hpy99I CGWCG 1 cut(s) 46
HpyCH4III ACNGT 5 cut(s) 296, 342, 1362, 1955, 2282
HpyCH4IV ACGT 3 cut(s) 41, 310, 2536
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 1532, 2135, 2315
HpySE526I ACGT 3 cut(s) 41, 310, 2536
HspAI GCGC 1 cut(s) 2819
Ksp22I TGATCA 2 cut(s) 1264, 2706
KspAI GTTAAC 1 cut(s) 1992
LguI GCTCTTC 1 cut(s) 2930
LmnI GCTCC 4 cut(s) 457, 888, 2053, 2173
Lsp1109I GCAGC 6 cut(s) 135, 218, 862, 1316, 2122, 2455
LweI GCATC 7 cut(s) 23, 238, 309, 348, 616, 1703, 2478
MaeI CTAG 4 cut(s) 237, 815, 1043, 1995
MaeII ACGT 3 cut(s) 41, 310, 2536
MbiI CCGCTC 1 cut(s) 2856
MfeI CAATTG 1 cut(s) 930
MflI RGATCY 4 cut(s) 226, 958, 2081, 2781
MhlI GDGCHC 1 cut(s) 1437
MlsI TGGCCA 3 cut(s) 1451, 2385, 2594
MluNI TGGCCA 3 cut(s) 1451, 2385, 2594
MlyI GAGTC 4 cut(s) 81, 310, 725, 1744
MmeI TCCRAC 4 cut(s) 117, 1318, 1333, 2151
Mox20I TGGCCA 3 cut(s) 1451, 2385, 2594
Mph1103I ATGCAT 2 cut(s) 1431, 2017
MroXI GAANNNNTTC 5 cut(s) 556, 708, 736, 2007, 2370
MscI TGGCCA 3 cut(s) 1451, 2385, 2594
MseI TTAA 8 cut(s) 351, 1136, 1910, 1923, 1991, 2019, 2058, 2255
MslI CAYNNNNRTG 1 cut(s) 2629
Msp20I TGGCCA 3 cut(s) 1451, 2385, 2594
MspA1I CMGCKG 1 cut(s) 2468
MspCI CTTAAG 1 cut(s) 2057
MspI CCGG 4 cut(s) 33, 2393, 2510, 2828
MspR9I CCNGG 2 cut(s) 20, 392
MunI CAATTG 1 cut(s) 930
Mva1269I GAATGC 1 cut(s) 1378
MvaI CCWGG 2 cut(s) 20, 392
MwoI GCNNNNNNNGC 4 cut(s) 11, 1532, 2135, 2315
NcoI CCATGG 2 cut(s) 682, 1073
NlaIV GGNNCC 4 cut(s) 960, 2083, 2461, 2783
NmeAIII GCCGAG 1 cut(s) 1507
NmuCI GTSAC 6 cut(s) 73, 311, 968, 2501, 2537, 2720
NsiI ATGCAT 2 cut(s) 1431, 2017
NspI RCATGY 4 cut(s) 1535, 2509, 2551, 2872
NspV TTCGAA 1 cut(s) 1052
PaeI GCATGC 1 cut(s) 1535
PagI TCATGA 2 cut(s) 1441, 1690
PciI ACATGT 1 cut(s) 2868
PciSI GCTCTTC 1 cut(s) 2930
PctI GAATGC 1 cut(s) 1378
PdmI GAANNNNTTC 5 cut(s) 556, 708, 736, 2007, 2370
PflFI GACNNNGTC 2 cut(s) 71, 1953
PkrI GCNGC 7 cut(s) 125, 208, 874, 877, 1306, 2137, 2470
PleI GAGTC 4 cut(s) 80, 309, 724, 1743
PpsI GAGTC 4 cut(s) 80, 309, 724, 1743
Ppu21I YACGTR 1 cut(s) 2537
PscI ACATGT 1 cut(s) 2868
PshAI GACNNNNGTC 1 cut(s) 684
Psp124BI GAGCTC 1 cut(s) 1437
Psp6I CCWGG 2 cut(s) 18, 390
PspFI CCCAGC 2 cut(s) 584, 2249
PspGI CCWGG 2 cut(s) 18, 390
PspN4I GGNNCC 4 cut(s) 960, 2083, 2461, 2783
PspPI GGNCC 2 cut(s) 1291, 2858
PstI CTGCAG 1 cut(s) 1161
PsuI RGATCY 4 cut(s) 226, 958, 2081, 2781
PsyI GACNNNGTC 2 cut(s) 71, 1953
PvuII CAGCTG 1 cut(s) 2468
RsaI GTAC 6 cut(s) 24, 309, 401, 813, 2948, 2954
RsaNI GTAC 6 cut(s) 23, 308, 400, 812, 2947, 2953
RseI CAYNNNNRTG 1 cut(s) 2629
SacI GAGCTC 1 cut(s) 1437
SapI GCTCTTC 1 cut(s) 2930
SaqAI TTAA 8 cut(s) 351, 1136, 1910, 1923, 1991, 2019, 2058, 2255
SatI GCNGC 7 cut(s) 124, 207, 873, 876, 1305, 2136, 2469
Sau96I GGNCC 2 cut(s) 1291, 2858
ScaI AGTACT 1 cut(s) 2954
SchI GAGTC 4 cut(s) 81, 310, 725, 1744
ScrFI CCNGG 2 cut(s) 20, 392
SduI GDGCHC 1 cut(s) 1437
SfaNI GCATC 7 cut(s) 23, 238, 309, 348, 616, 1703, 2478
SfcI CTRYAG 1 cut(s) 1157
SfuI TTCGAA 1 cut(s) 1052
SinI GGWCC 2 cut(s) 1291, 2858
SmiMI CAYNNNNRTG 1 cut(s) 2629
SmlI CTYRAG 7 cut(s) 713, 1717, 1871, 2057, 2102, 2330, 2399
SmoI CTYRAG 7 cut(s) 713, 1717, 1871, 2057, 2102, 2330, 2399
SpeI ACTAGT 2 cut(s) 236, 1994
SphI GCATGC 1 cut(s) 1535
SsiI CCGC 3 cut(s) 720, 872, 2856
SspI AATATT 1 cut(s) 191
SspMI CTAG 4 cut(s) 237, 815, 1043, 1995
SstI GAGCTC 1 cut(s) 1437
StyD4I CCNGG 2 cut(s) 18, 390
StyI CCWWGG 4 cut(s) 682, 1073, 1327, 2481
TaaI ACNGT 5 cut(s) 296, 342, 1362, 1955, 2282
TaiI ACGT 3 cut(s) 44, 313, 2539
TaqI TCGA 6 cut(s) 29, 222, 317, 910, 1052, 1382
TatI WGTACW 2 cut(s) 811, 2952
TauI GCSGC 1 cut(s) 875
Tru1I TTAA 8 cut(s) 351, 1136, 1910, 1923, 1991, 2019, 2058, 2255
Tru9I TTAA 8 cut(s) 351, 1136, 1910, 1923, 1991, 2019, 2058, 2255
TscAI CASTG 3 cut(s) 115, 301, 2236
TseFI GTSAC 6 cut(s) 73, 311, 968, 2501, 2537, 2720
TseI GCWGC 6 cut(s) 123, 206, 875, 1304, 2135, 2468
Tsp45I GTSAC 6 cut(s) 73, 311, 968, 2501, 2537, 2720
TspRI CASTG 3 cut(s) 115, 301, 2236
Tth111I GACNNNGTC 2 cut(s) 71, 1953
Vha464I CTTAAG 1 cut(s) 2057
VpaK11BI GGWCC 2 cut(s) 1291, 2858
XapI RAATTY 8 cut(s) 80, 644, 918, 1105, 1622, 1694, 1729, 1774
XceI RCATGY 4 cut(s) 1535, 2509, 2551, 2872
XmiI GTMKAC 1 cut(s) 618
XmnI GAANNNNTTC 5 cut(s) 556, 708, 736, 2007, 2370
XspI CTAG 4 cut(s) 237, 815, 1043, 1995
ZrmI AGTACT 1 cut(s) 2954
Zsp2I ATGCAT 2 cut(s) 1431, 2017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.