Rroxscaffold_2G00103050

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001339
Physical Location & Seq
Reverse (-)
25499516 .. 25504042
4527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00103050.1

Sequence Viewer

Length: 2868 bp
ATGGCGACGACTATAGCGTCTCTAGTACTTTCCACCGGCATCACTTCGGCAGTTCGTCAGGTTCTTCTCGACAGAATTGCCAATTTCACTCAAACAAAGATAACCCAGTTTGCCCAGCTGCACGTCGAGTTCTTGAAGTTGGGAGCAACAATGTCCAAGGCTAAAGCTCTTGTTGATGATGTAGAGAACAAGAAATGTTTTCCAGAGGCTTGCCGAATTTTGCTCGAAGATGTCAAAAGTGTTGTTTTAGATGCCGATGATTTGCTGGATGAGATTGACATGGACCTCGAAAATAGGAATTCTACCGACTTCAACAACAATCAGGTTAGTCAAATGTTCCTATCATCTCGTAGATTCAATATTCCTTCTGAGCTAAGCAAGATCCATAAAGAACTGGAGGGTTTAGTAGAGAAACTGGAGGGCTACTGTATGATAAATTCGGGTCAACCTCAAATGGCTGCTCCTGTTATTCCCAGTACTTCTTTAGTGGATGAGTCATCTATAATTGCAAGAGTTTCAGACAAGGAAAAGATCATTCAGATGGTCTTGTCTGACCAAGGAGAGGATGGAGGAAATGTTTCTGTGATTCCAATAGTGGGAATGGGCGGAATTGGGAAAACAGCACTAGCTCAACTTGTTTACAATGATCCAGTTGTGGCTAAAAATTTCGATTTTAAAATCTGGGTATCTGTTTCTTTGAGCTGTGATGTTGTCAAGATTACAAAATCCATTTTTCACTCTCTTACTGAGCAAGTGTCTGGGTTGTCTGAACTCAAGAGAGCTTTTGAGTTATCTGATCTAAGCAAATATTTTGAGTTGAGTCTTAATGAGATTCATATTCAGTTACAAAAGAAGTTGAGGGGGAAGAAGTTTTTGCTTGTACTTGACGATATGTGGAATGAGAATCCTAGAGATTGGGATGTATTGAATTTGCCTTTTCAAGTTGCAGCACAAGGAAGCAAGATTGTGATGACTACCCGAAGCAAAATTGCTTCGTCTATTCTTTCTGGTGCTTCTGCTTATCATTTGGATACTTTGTCTCATAATGATTGCTGGACAATCATTAAACAGACGGCAGGACTAAGTGATGCACATGAAAATGAATTGATTGGGTTAAAGATAGCTAAGAAGTGCAAAGGGCTTCCTTTGGCGGCAAAAGTGATTGGAAGTGTCTTACGTTATAAATCTGGAGACAGGAAGTGGGATGCTTTGTTAAAATGTGAGCTTTGGGAATCTCCAGAACATGGAAACCATATCTATCCAGCACTTAAGTTGAGCTATGATAATTTGTCTTCTTATTTGAGAAGATGTTTCACTTATTGTTCTATCTTCCCACGTGATCATGAATTTGAAAAAGATGATTTGGTTCAGTTATGGGCAGCAGAAGGCTTCATTCAAGCTCAAGGAAGAGAAGCAATTGAAGACGTTGGAAGAGAGTACTTTGTTGGGTTGTGTTCGAGGTCTTTTTTCCAAGTTTCAGATGATATTCAACCAAGATATAAAATGCATGACCTCATTCATGATTTGGCGAGATTGATTTCAGCCAACATTTCCTTTTCTATGGAGGATAATATCTCAACCTTCTTGCCCATACCTAGACATGCTCGCTATTCATCATTGCTTTGTCAAGACATTAAGCCATCTACATTGGAGGTGTTTCACAGGTATCAGAAGTTGCGAACCTTTATGCTGCTTTCTGAACATGCATCCAATCTTTACCAAGTTCCATATGATTTCTTTTTAAAGTTGGGACGCTTAAGAGTGTTGAATCTGAGTCATAGTGGCATCTCTGACTTGCCTGACTCTATTAGGAACTTGATACACCTCCGTTACCTAAATCTTAGTCATACCTCTATCAAAAGATTGCCAAAATCTATGGCAAATATTCATGGCTTGGAAACACTGAAGCTCAAGAATTGTTGTGAGCTTCTTCAATTGCCTGACAACTTGAAGAACTTGATTAAGCTACGACATCTTGACTTTGATGGATATCGTTTAATCAGTTCGATGCCATTAGAAGTTGGGAAACTGACCAGTCTTGAAACTCTGCCTGCATTTATAGTGGGAAAGCACAAAGGGAATCAAATTGGGGAGCTCAAGAACATGAGGTTCCTTCGTGGGTCCATTTGCATCACAAATCTTGAGAATGTGGCAAGTTCTATAGAGGGTGAGGCTGCTATGTTACACGAGAAGCAATACCTAGAGAAGTTGGAGTTAGAATGGAATGAAATGGGTGCTCAAACAGTTGAACAAGAAGTTCTTACGAGCCTTAAACCTCATGTTGGGTTGAGAGAGTTACGAGTAACAGGCTATTGTGGTTTAATATTTCCAAATTGGATTGCTGACTCATCATTTTGCAAGCTTGAGAGAATCGACATAGACAAGTGCCATTTTTGTACACTTCTTCCTTCTCTTGGTCAACTTCCGGCTCTCAAGTATCTCCTCATTCAAGATATGCGTGCTTTGGAAAATGTGGATCATCTGTTATGTGGATCGGCAGGTAGTTTTCCGTCATTGGAGACGCTCACATTTCGGGACATGCCAAGGTTGAAGATTTGGTCAGGAATAAATGAAAATGACATGCCTCACCTTAGAGTGCTAAGCATTGATTCTTGTCCAGAATTGGTCACACTACCTTCAACACATTCCCTAAGATCACTTGAAGTGTTAGAAATAAATCGTTGCCCTGAGCTTCAATCATTGCCAAGGGAAGGACTGCCATTCTCACTCACATGTCTGATAATTTCAGAAAGTGATATTTTAAAGGAGCGATGCGAGGTGCAAGGTGCAGATTGGGACAAGATCAAATCAGTCCCCAACATATTGATTGATGATGAAGTGATTCCCATGGAAGGACGTCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

955

Amino Acids

107.81

Weight (kDa)

5.98

Isoelectric Point (pI)

35.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 21 - 105 8.7e-09 Rx N-terminal domain
NB-ARC PF00931 176 - 360 3.4e-31 NB-ARC domain
WHD_DRP PF23559 443 - 510 1.1e-26 Disease resistance protein Winged helix domain
LRR_8 PF13855 570 - 619 1.2e-06 Leucine rich repeat
LRR_14 PF23598 582 - 869 3.2e-32 Leucine-rich repeat region
LRR_8 PF13855 585 - 640 1.8e-07 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 696 - 819 2.7e-35 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 781 - 895 4.6e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1182
AasI GACNNNNNNGTC 1 cut(s) 16
AatII GACGTC 1 cut(s) 2860
Acc36I ACCTGC 1 cut(s) 2489
AccB7I CCANNNNNTGG 1 cut(s) 1244
AciI CCGC 2 cut(s) 606, 1151
AclWI GGATC 4 cut(s) 376, 641, 2484, 2500
AcsI RAATTY 6 cut(s) 216, 298, 436, 664, 928, 1346
AcuI CTGAAG 1 cut(s) 1925
AcvI CACGTG 1 cut(s) 1337
AcyI GRCGYC 1 cut(s) 2857
AfaI GTAC 5 cut(s) 27, 478, 882, 1439, 2398
AfiI CCNNNNNNNGG 7 cut(s) 562, 596, 1244, 2282, 2414, 2711, 2852
AflII CTTAAG 2 cut(s) 1268, 1756
AflIII ACRYGT 1 cut(s) 2732
AjiI CACGTC 1 cut(s) 124
Alw21I GWGCWC 2 cut(s) 2097, 2239
Alw26I GTCTC 4 cut(s) 24, 1044, 1185, 2513
AlwI GGATC 4 cut(s) 376, 641, 2484, 2500
ApeKI GCWGC 6 cut(s) 118, 458, 947, 1379, 1690, 2174
ApoI RAATTY 6 cut(s) 216, 298, 436, 664, 928, 1346
Asp700I GAANNNNTTC 2 cut(s) 577, 2841
AspS9I GGNCC 2 cut(s) 283, 2121
AsuHPI GGTGA 2 cut(s) 2180, 2579
AvaII GGWCC 2 cut(s) 283, 2121
BanII GRGCYC 1 cut(s) 2097
BauI CACGAG 1 cut(s) 2186
BbrPI CACGTG 1 cut(s) 1337
BbsI GAAGAC 2 cut(s) 1284, 1428
Bbv12I GWGCWC 2 cut(s) 2097, 2239
BbvI GCAGC 6 cut(s) 105, 445, 959, 1391, 1677, 2161
BccI CCATC 4 cut(s) 535, 560, 1648, 1979
BceAI ACGGC 1 cut(s) 1089
BcgI CGANNNNNNTGC 2 cut(s) 59, 93
BciVI GTATCC 1 cut(s) 1024
BclI TGATCA 1 cut(s) 1339
BcoDI GTCTC 4 cut(s) 24, 1044, 1185, 2513
BfaI CTAG 5 cut(s) 23, 626, 909, 1596, 2201
BfmI CTRYAG 2 cut(s) 12, 2160
BfrI CTTAAG 2 cut(s) 1268, 1756
BfuAI ACCTGC 1 cut(s) 2489
BfuI GTATCC 1 cut(s) 1024
BisI GCNGC 7 cut(s) 119, 459, 948, 1152, 1380, 1691, 2175
BlpI GCTNAGC 2 cut(s) 374, 2600
BlsI GCNGC 7 cut(s) 120, 460, 949, 1153, 1381, 1692, 2176
BmcAI AGTACT 3 cut(s) 27, 478, 1439
Bme18I GGWCC 2 cut(s) 283, 2121
BmgBI CACGTC 1 cut(s) 124
BmgT120I GGNCC 2 cut(s) 283, 2121
BmiI GGNNCC 2 cut(s) 2111, 2122
BmrI ACTGGG 2 cut(s) 100, 468
BmsI GCATC 9 cut(s) 48, 241, 1078, 1195, 1715, 1794, 1998, 2139, 2762
BmuI ACTGGG 2 cut(s) 100, 468
BpiI GAAGAC 2 cut(s) 1284, 1428
BpmI CTGGAG 4 cut(s) 416, 437, 1209, 1221
Bpu10I CCTNAGC 1 cut(s) 2688
Bpu1102I GCTNAGC 2 cut(s) 374, 2600
BpuEI CTTGAG 7 cut(s) 758, 1386, 1895, 2081, 2162, 2384, 2417
BsaAI YACGTR 1 cut(s) 1337
BsaBI GATNNNNATC 1 cut(s) 1989
BsaHI GRCGYC 1 cut(s) 2857
BsaJI CCNNGG 5 cut(s) 156, 556, 2543, 2705, 2847
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 7 cut(s) 562, 596, 1244, 2282, 2414, 2711, 2852
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 6 cut(s) 106, 399, 420, 474, 650, 2034
Bse3DI GCAATG 2 cut(s) 1616, 2699
Bse8I GATNNNNATC 1 cut(s) 1989
BseDI CCNNGG 5 cut(s) 156, 556, 2543, 2705, 2847
BseGI GGATG 6 cut(s) 274, 496, 571, 925, 1210, 1706
BseJI GATNNNNATC 1 cut(s) 1989
BseLI CCNNNNNNNGG 7 cut(s) 562, 596, 1244, 2282, 2414, 2711, 2852
BseMI GCAATG 2 cut(s) 1616, 2699
BseMII CTCAG 4 cut(s) 360, 738, 1763, 2679
BseNI ACTGG 6 cut(s) 106, 399, 420, 474, 650, 2034
BseRI GAGGAG 1 cut(s) 2432
BseXI GCAGC 6 cut(s) 105, 445, 959, 1391, 1677, 2161
BseYI CCCAGC 1 cut(s) 114
BsgI GTGCAG 2 cut(s) 104, 2808
BsiHKAI GWGCWC 2 cut(s) 2097, 2239
BsiSI CCGG 2 cut(s) 36, 2426
BslFI GGGAC 4 cut(s) 1764, 2549, 2798, 2810
BslI CCNNNNNNNGG 7 cut(s) 562, 596, 1244, 2282, 2414, 2711, 2852
BsmAI GTCTC 4 cut(s) 24, 1044, 1185, 2513
BsmBI CGTCTC 2 cut(s) 24, 2513
BsmFI GGGAC 4 cut(s) 1764, 2549, 2798, 2810
Bsp1286I GDGCHC 2 cut(s) 2097, 2239
Bsp1407I TGTACA 1 cut(s) 2396
Bsp143I GATC 9 cut(s) 381, 531, 646, 796, 1339, 2476, 2492, 2654, 2802
Bsp1720I GCTNAGC 2 cut(s) 374, 2600
Bsp19I CCATGG 1 cut(s) 2847
BspACI CCGC 2 cut(s) 606, 1151
BspCNI CTCAG 4 cut(s) 361, 739, 1764, 2680
BspHI TCATGA 2 cut(s) 1342, 1519
BspLI GGNNCC 2 cut(s) 2111, 2122
BspMI ACCTGC 1 cut(s) 2489
BspPI GGATC 4 cut(s) 376, 641, 2484, 2500
BspTI CTTAAG 2 cut(s) 1268, 1756
BsrDI GCAATG 2 cut(s) 1616, 2699
BsrFI RCCGGY 1 cut(s) 35
BsrGI TGTACA 1 cut(s) 2396
BsrI ACTGG 6 cut(s) 106, 399, 420, 474, 650, 2034
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 5 cut(s) 156, 556, 2543, 2705, 2847
BssMI GATC 9 cut(s) 381, 531, 646, 796, 1339, 2476, 2492, 2654, 2802
BssNI GRCGYC 1 cut(s) 2857
BssSI CACGAG 1 cut(s) 2186
BssT1I CCWWGG 5 cut(s) 156, 556, 2543, 2705, 2847
Bst2BI CACGAG 1 cut(s) 2186
Bst4CI ACNGT 2 cut(s) 428, 2245
Bst6I CTCTTC 2 cut(s) 1402, 1426
BstACI GRCGYC 1 cut(s) 2857
BstAFI CTTAAG 2 cut(s) 1268, 1756
BstAUI TGTACA 1 cut(s) 2396
BstBAI YACGTR 1 cut(s) 1337
BstC8I GCNNGC 5 cut(s) 211, 1606, 2052, 2360, 2460
BstDSI CCRYGG 1 cut(s) 2847
BstF5I GGATG 6 cut(s) 274, 496, 571, 925, 1210, 1706
BstKTI GATC 9 cut(s) 384, 534, 649, 799, 1342, 2479, 2495, 2657, 2805
BstMAI GTCTC 4 cut(s) 24, 1044, 1185, 2513
BstMBI GATC 9 cut(s) 381, 531, 646, 796, 1339, 2476, 2492, 2654, 2802
BstNSI RCATGY 5 cut(s) 1604, 1706, 2542, 2584, 2736
BstSFI CTRYAG 2 cut(s) 12, 2160
BstV1I GCAGC 6 cut(s) 105, 445, 959, 1391, 1677, 2161
BstV2I GAAGAC 2 cut(s) 1284, 1428
BstX2I RGATCY 1 cut(s) 381
BstYI RGATCY 1 cut(s) 381
BsuI GTATCC 1 cut(s) 1024
BtgI CCRYGG 1 cut(s) 2847
BtgZI GCGATG 1 cut(s) 2785
BtrI CACGTC 1 cut(s) 124
BtsCI GGATG 6 cut(s) 274, 496, 571, 925, 1210, 1706
BtsIMutI CAGTG 1 cut(s) 1901
BveI ACCTGC 1 cut(s) 2489
Cac8I GCNNGC 5 cut(s) 211, 1606, 2052, 2360, 2460
CciI TCATGA 2 cut(s) 1342, 1519
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 2 cut(s) 283, 2121
CseI GACGC 3 cut(s) 6, 1761, 2530
Csp6I GTAC 5 cut(s) 26, 477, 881, 1438, 2397
CviQI GTAC 5 cut(s) 26, 477, 881, 1438, 2397
DpnI GATC 9 cut(s) 383, 533, 648, 798, 1341, 2478, 2494, 2656, 2804
DpnII GATC 9 cut(s) 381, 531, 646, 796, 1339, 2476, 2492, 2654, 2802
DraI TTTAAA 3 cut(s) 676, 1743, 2763
DrdI GACNNNNNNGTC 1 cut(s) 16
DseDI GACNNNNNNGTC 1 cut(s) 16
Eam1104I CTCTTC 2 cut(s) 1402, 1426
EarI CTCTTC 2 cut(s) 1402, 1426
EciI GGCGGA 1 cut(s) 621
Ecl136II GAGCTC 1 cut(s) 2095
Eco130I CCWWGG 5 cut(s) 156, 556, 2543, 2705, 2847
Eco24I GRGCYC 1 cut(s) 2097
Eco32I GATATC 1 cut(s) 1991
Eco47I GGWCC 2 cut(s) 283, 2121
Eco53kI GAGCTC 1 cut(s) 2095
Eco57I CTGAAG 1 cut(s) 1925
Eco72I CACGTG 1 cut(s) 1337
EcoICRI GAGCTC 1 cut(s) 2095
EcoRI GAATTC 1 cut(s) 298
EcoRV GATATC 1 cut(s) 1991
EcoT14I CCWWGG 5 cut(s) 156, 556, 2543, 2705, 2847
EcoT22I ATGCAT 2 cut(s) 1509, 1708
EcoT38I GRGCYC 1 cut(s) 2097
ErhI CCWWGG 5 cut(s) 156, 556, 2543, 2705, 2847
Esp3I CGTCTC 2 cut(s) 24, 2513
FalI AAGNNNNNCTT 2 cut(s) 2244, 2276
FaqI GGGAC 4 cut(s) 1764, 2549, 2798, 2810
FauNDI CATATG 1 cut(s) 1729
FbaI TGATCA 1 cut(s) 1339
Fnu4HI GCNGC 7 cut(s) 119, 459, 948, 1152, 1380, 1691, 2175
FokI GGATG 6 cut(s) 281, 503, 578, 932, 1217, 1693
FriOI GRGCYC 1 cut(s) 2097
Fsp4HI GCNGC 7 cut(s) 119, 459, 948, 1152, 1380, 1691, 2175
FspBI CTAG 5 cut(s) 23, 626, 909, 1596, 2201
GluI GCNGC 7 cut(s) 119, 459, 948, 1152, 1380, 1691, 2175
GsaI CCCAGC 1 cut(s) 118
GsuI CTGGAG 4 cut(s) 416, 437, 1209, 1221
HapII CCGG 2 cut(s) 36, 2426
HgaI GACGC 3 cut(s) 6, 1761, 2530
Hin1I GRCGYC 1 cut(s) 2857
HincII GTYRAC 2 cut(s) 446, 2420
HindII GTYRAC 2 cut(s) 446, 2420
HindIII AAGCTT 1 cut(s) 2360
HpaII CCGG 2 cut(s) 36, 2426
HphI GGTGA 2 cut(s) 2180, 2579
Hpy166II GTNNAC 4 cut(s) 446, 640, 2399, 2420
Hpy8I GTNNAC 4 cut(s) 446, 640, 2399, 2420
Hpy99I CGWCG 2 cut(s) 10, 128
HpyAV CCTTC 8 cut(s) 375, 1379, 1591, 2123, 2418, 2646, 2705, 2846
HpyCH4III ACNGT 2 cut(s) 428, 2245
HpyCH4IV ACGT 5 cut(s) 123, 1177, 1336, 1425, 2857
HpySE526I ACGT 5 cut(s) 123, 1177, 1336, 1425, 2857
Hsp92I GRCGYC 1 cut(s) 2857
Ksp22I TGATCA 1 cut(s) 1339
Kzo9I GATC 9 cut(s) 381, 531, 646, 796, 1339, 2476, 2492, 2654, 2802
LmnI GCTCC 4 cut(s) 143, 466, 2092, 2767
Lsp1109I GCAGC 6 cut(s) 105, 445, 959, 1391, 1677, 2161
LweI GCATC 9 cut(s) 48, 241, 1078, 1195, 1715, 1794, 1998, 2139, 2762
MaeI CTAG 5 cut(s) 23, 626, 909, 1596, 2201
MaeII ACGT 5 cut(s) 123, 1177, 1336, 1425, 2857
MaeIII GTNAC 6 cut(s) 843, 1829, 2181, 2295, 2302, 2626
MalI GATC 9 cut(s) 383, 533, 648, 798, 1341, 2478, 2494, 2656, 2804
MboI GATC 9 cut(s) 381, 531, 646, 796, 1339, 2476, 2492, 2654, 2802
MfeI CAATTG 2 cut(s) 1416, 1934
MflI RGATCY 1 cut(s) 381
MhlI GDGCHC 2 cut(s) 2097, 2239
MlyI GAGTC 5 cut(s) 503, 829, 1783, 1796, 2339
MmeI TCCRAC 2 cut(s) 1408, 2190
Mph1103I ATGCAT 2 cut(s) 1509, 1708
MroXI GAANNNNTTC 2 cut(s) 577, 2841
MslI CAYNNNNRTG 3 cut(s) 539, 1098, 2731
MspA1I CMGCKG 1 cut(s) 118
MspCI CTTAAG 2 cut(s) 1268, 1756
MspI CCGG 2 cut(s) 36, 2426
MunI CAATTG 2 cut(s) 1416, 1934
NcoI CCATGG 1 cut(s) 2847
NdeI CATATG 1 cut(s) 1729
NdeII GATC 9 cut(s) 381, 531, 646, 796, 1339, 2476, 2492, 2654, 2802
NlaIV GGNNCC 2 cut(s) 2111, 2122
NmuCI GTSAC 1 cut(s) 2626
NsiI ATGCAT 2 cut(s) 1509, 1708
NspI RCATGY 5 cut(s) 1604, 1706, 2542, 2584, 2736
PagI TCATGA 2 cut(s) 1342, 1519
PciI ACATGT 1 cut(s) 2732
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PdmI GAANNNNTTC 2 cut(s) 577, 2841
PflMI CCANNNNNTGG 1 cut(s) 1244
PkrI GCNGC 7 cut(s) 120, 460, 949, 1153, 1381, 1692, 2176
PleI GAGTC 5 cut(s) 502, 828, 1782, 1796, 2339
PmaCI CACGTG 1 cut(s) 1337
PmlI CACGTG 1 cut(s) 1337
PpsI GAGTC 5 cut(s) 502, 828, 1782, 1796, 2339
Ppu21I YACGTR 1 cut(s) 1337
PscI ACATGT 1 cut(s) 2732
PsiI TTATAA 1 cut(s) 1182
Psp124BI GAGCTC 1 cut(s) 2097
PspCI CACGTG 1 cut(s) 1337
PspFI CCCAGC 1 cut(s) 114
PspN4I GGNNCC 2 cut(s) 2111, 2122
PspPI GGNCC 2 cut(s) 283, 2121
PsuI RGATCY 1 cut(s) 381
PvuII CAGCTG 1 cut(s) 118
RsaI GTAC 5 cut(s) 27, 478, 882, 1439, 2398
RsaNI GTAC 5 cut(s) 26, 477, 881, 1438, 2397
RseI CAYNNNNRTG 3 cut(s) 539, 1098, 2731
SacI GAGCTC 1 cut(s) 2097
SatI GCNGC 7 cut(s) 119, 459, 948, 1152, 1380, 1691, 2175
Sau3AI GATC 9 cut(s) 381, 531, 646, 796, 1339, 2476, 2492, 2654, 2802
Sau96I GGNCC 2 cut(s) 283, 2121
ScaI AGTACT 3 cut(s) 27, 478, 1439
SchI GAGTC 5 cut(s) 503, 829, 1783, 1796, 2339
SduI GDGCHC 2 cut(s) 2097, 2239
SfaNI GCATC 9 cut(s) 48, 241, 1078, 1195, 1715, 1794, 1998, 2139, 2762
SfcI CTRYAG 2 cut(s) 12, 2160
SinI GGWCC 2 cut(s) 283, 2121
SmiMI CAYNNNNRTG 3 cut(s) 539, 1098, 2731
SmlI CTYRAG 9 cut(s) 773, 1268, 1401, 1756, 1910, 2096, 2141, 2363, 2432
SmoI CTYRAG 9 cut(s) 773, 1268, 1401, 1756, 1910, 2096, 2141, 2363, 2432
SsiI CCGC 2 cut(s) 606, 1151
SspI AATATT 4 cut(s) 361, 809, 1885, 2325
SspMI CTAG 5 cut(s) 23, 626, 909, 1596, 2201
SstI GAGCTC 1 cut(s) 2097
StyI CCWWGG 5 cut(s) 156, 556, 2543, 2705, 2847
TaaI ACNGT 2 cut(s) 428, 2245
TaiI ACGT 5 cut(s) 126, 1180, 1339, 1428, 2860
TaqI TCGA 8 cut(s) 69, 126, 225, 288, 669, 1457, 2006, 2373
TatI WGTACW 5 cut(s) 25, 476, 880, 1437, 2396
TauI GCSGC 1 cut(s) 1154
TscAI CASTG 1 cut(s) 1908
TseFI GTSAC 1 cut(s) 2626
TseI GCWGC 6 cut(s) 118, 458, 947, 1379, 1690, 2174
Tsp45I GTSAC 1 cut(s) 2626
TspGWI ACGGA 2 cut(s) 1817, 2499
TspRI CASTG 1 cut(s) 1908
Van91I CCANNNNNTGG 1 cut(s) 1244
Vha464I CTTAAG 2 cut(s) 1268, 1756
VpaK11BI GGWCC 2 cut(s) 283, 2121
XapI RAATTY 6 cut(s) 216, 298, 436, 664, 928, 1346
XceI RCATGY 5 cut(s) 1604, 1706, 2542, 2584, 2736
XcmI CCANNNNNNNNNTGG 1 cut(s) 563
XmnI GAANNNNTTC 2 cut(s) 577, 2841
XspI CTAG 5 cut(s) 23, 626, 909, 1596, 2201
ZraI GACGTC 1 cut(s) 2858
ZrmI AGTACT 3 cut(s) 27, 478, 1439
Zsp2I ATGCAT 2 cut(s) 1509, 1708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.