Rw4G027820

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001339
Physical Location & Seq
Reverse (-)
54531060 .. 54535331
4272 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G027820.1

Sequence Viewer

Length: 2979 bp
ATGGCAGAGATAGCATCCCTGGTACTCTCGACCGGCATTACGTCGGCAATTCTTCAGGTTGTTGTTGACAGAGTCGCAAAATTTGTTGAACAAAAGAGAGACATGTTCACTGGGGTCGACGACAATCTCAGAAAGTTGAAGAGAACGCTGATGAAGATTCAAGCTCGTGTTGATGATATAGAAAAGAAACATTGCATCACGGAGGCAGCAGAAGATTTACTGGAAGATCTGCTGACTAGTTTTTTGGATGCTGAAGATTTGCTGGACAAGATCGGTTTGGGACTGGAAAGGATGACTTGTTTGGATGTGGAGGTCAACAGTTGGAGTCAGGTACATCACTTGATCCCCTTCTATTCTGGCATACCGTCTGAGATTAAAAAGATGCAAGTGAAATTGGATGAGTTAGTAACAGAACTAGAAAGCTACCCTACCACGGAAGTCATCCGGTTGAGAAAGCCTATAGAAAAGGCTATTGCTCCTTGTACTTCTTTGGTTGATGAATCTTGTGTATTTGGAAGGGAAACAGATAAGAACAAGATGAAAAAAATGGTCTTGTCTCTGCAAGAAGAGGGGGGAAATGTTTCTGTAATTTCAATAGTAGGGATGGCTGGGATTGGGAAAACAACACTTGCCCAACTCATCTACAATGATGCAGATGTGCATAAGAAATTTGATTTGAAAATGTGGGTGTCTGTTTCTTTAGAGTGTGGTCTGTACATGATTACAAAATCTATTCTTGAGTCTGCTACTAGAGGAAAGGGTTCTCAGTTGTCAAGTTTGGATAGTGTTCAGATTGAATTACAGAAGACATTGAAGGGGAAGAGGTTTTTACTTGTACTAGATGGCATTTGTAATGATAATCTTAGTGATTGGGATCAATTCAGGTTGTCTTTTGGGGCTGCAAAGAAGGGGAGCAAGATTATGATGACTACTCGAAGTGAAATTTCTGCCTCAGTTGTTGCCTCTACATTATATCGTTTGGATCCTCTGTGTGACAATGCTTGTTGGGAAATAATCAAACAAAGAGGGAATCTTAATGGGCAGACTAATTTGGAGGCGAATGGCCTAGCAATTTCGAAGAAGTGCAAAGGGTTTCCATGGGTAGCAAAAGTGATTGGAAGCTGTTTGAATTCGGGAGAGATGGAGTGGGATGCTCTGTTAAGGTGTGAGCTTTGGGAACTGCAGGAACATAAAACACAAATCTATCCGGTCCTCAAATTGTTGAGCTATGATAATATGCCTTCTTATTTGAAAAGATGTTTCAGTTATTGTTCTATCTTTCCTCGTGATCATGTAGTTGAGAAGGATGATTTGGTCCAACTATGGGCAGCAGAAGGCTTTATTCAGTCCCAAGGAACAAGAAAAATTGAAGATGTTGGAAGACAGTATTTTGAGGACTTGTGTTCGAGGGCTTTCTTTTCACAAGTTCCAAATATTGGTGGTGATCTGCAAAGATATAAAATGCATGAGCTCATTCATGATTTGGCAAGATTTGTTTCAACCAACTTATGCTTTTGTATGGATGACAATGTGCCATGCTTGGTGCACATATCTCCTAATGCTCGGCATGCGTCTTTGCTCTATGGAAATACTCAACTATCTACATTGGAGGCTTTCTACGAGTATAAGAAGTTGAGGACATTTACATTGCTTCCTAAGTCCCCATCTATTCCGAGGCCAGTCATGTTGCTTCCCAATTTTTCGTCCAATCTTGGCGAAGTCCCTCATGAATTTTTTGAAAGGTTGGGAAGCTTGAGGGTGTTGAATTTGAGTCATAGTGCCATCTCTAAATTGCCTGAATCTATTGGAAATTTGATACATCTTCGTTACCTCAATCTTAGTCATACCCGTATTGAACTATTGCCAAAGCCTATAACAACAATATATGGGTTAGAAACATTGAAGCTCAAGAGTTGTCCTAAGCTTCTTCATTTGCCTGAGAACTTAAAGGACTTGATTAAGCTAAGACATCTTGACTTTGACAGACACCCGTCAACTAATCTCGAAACCCTTCATGCATTTAAAGTGGGAAAAGAGAAAGGATATCAGATTGAGGAGCTCAAGAACATGAGGTGTCTTCGTGGATCCATTTCTATTACGAATCTTGAGAATGTGGCAGATTCTATGCAGGCTGAGGCTGCTATGTTACACAACAAGCAATACTTAGACAGGTTGGAGCTGGAATGGAATGCAATCAGAGGTCAAATGCAACCAGTTCAACAAGAAGTTCTTACAGGGCTTAAACCACATGCTGGGTTGAAGGAGTTACGAGTAGCTGGTTACTGTGGTTCGGTATTTCCCGGATGGATAGCCAACTCAGCATTTAGCAAGCTTGAGAGTATTGAATTAGAAAATTGCGCATTTTGTGAACTCCTTCCTTCTCTTGGCCAACTTCCGGCTCTCAAGAAACTTCTTATTCAAAATATGGCTACTTTGAAAGGTGTGGATTATCTGTTTTGTGGTTCCTCAGCTGCTTTTCTATCCTTAGAGACGCTAACACTTCGTGACATGCCGGAGTTGAGTAACTGGTCAGGACTACGTGACAATGACATGCCTTTCCTCAGAGACCTCAGCATTGATTCTTGTCCAAAGTTGGCCACCCTACCTTCATTTCATTACCTAACTTCACTTCAATGTTTAGACATCAATCGTTGCCCTCAGCTTCAATCTTTGCCAGAGGGAGGACTGCCATCCTCACTCAAAACTTTGGTCATTATGGAGAGTGGCATCTTGAAGGAACGGTGCAAGGTGGGAGAAGGTGCAGATTGGAATAAGATCAGATGGATCCCTGCCATATTGATTGAAAATATGGTGATTCCAGCACAAAGACCGGATAACCAGATTGATGAGGTTCAGAGTGCTCCAGAAAACATGTTTTGCAGCCGAAACTCCTTGTTGACTTGTTCTGTTATTCTTTTTCCTCTGCTCTTCCTTTTTATGAGATCATTGTACCAGTACTTGTCCCAAGCCAGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

992

Amino Acids

111.94

Weight (kDa)

6.44

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 18 - 98 7.8e-10 Rx N-terminal domain
NB-ARC PF00931 179 - 343 8.4e-31 NB-ARC domain
WHD_DRP PF23559 426 - 496 5.4e-25 Disease resistance protein Winged helix domain
LRR_8 PF13855 568 - 618 5.3e-09 Leucine rich repeat
LRR_14 PF23598 581 - 861 1.2e-23 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 684 - 809 6.6e-38 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 772 - 885 6.2e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2359
AccB7I CCANNNNNTGG 2 cut(s) 1436, 2252
AccI GTMKAC 1 cut(s) 117
AclWI GGATC 8 cut(s) 337, 882, 977, 990, 2079, 2092, 2779, 2792
AcoI YGGCCR 2 cut(s) 2386, 2595
AcsI RAATTY 7 cut(s) 80, 668, 942, 1129, 1730, 1765, 1810
AcuI CTGAAG 2 cut(s) 38, 273
AdeI CACNNNGTG 1 cut(s) 2504
AfaI GTAC 7 cut(s) 24, 333, 484, 716, 837, 2951, 2957
AfiI CCNNNNNNNGG 7 cut(s) 433, 1324, 1436, 2252, 2384, 2395, 2681
AflIII ACRYGT 2 cut(s) 102, 2871
AhlI ACTAGT 1 cut(s) 236
AjnI CCWGG 1 cut(s) 18
AjuI GAANNNNNNNTTGG 2 cut(s) 1295, 1327
Alw21I GWGCWC 4 cut(s) 1473, 1548, 2061, 2863
Alw26I GTCTC 4 cut(s) 93, 561, 2483, 2559
Alw44I GTGCAC 1 cut(s) 1544
AlwI GGATC 8 cut(s) 337, 882, 977, 990, 2079, 2092, 2779, 2792
AoxI GGCC 4 cut(s) 1063, 1676, 2386, 2595
ApaLI GTGCAC 1 cut(s) 1544
ApeKI GCWGC 6 cut(s) 206, 899, 1328, 2138, 2471, 2880
ApoI RAATTY 7 cut(s) 80, 668, 942, 1129, 1730, 1765, 1810
ArsI GACNNNNNNTTYG 4 cut(s) 226, 258, 2694, 2726
Asp700I GAANNNNTTC 4 cut(s) 580, 760, 2010, 2373
AspLEI GCGC 1 cut(s) 2360
AspS9I GGNCC 2 cut(s) 1210, 1315
AsuC2I CCSGG 1 cut(s) 2301
AsuHPI GGTGA 2 cut(s) 1454, 2824
AsuII TTCGAA 1 cut(s) 1076
AvaII GGWCC 2 cut(s) 1210, 1315
BaeGI GKGCMC 1 cut(s) 1548
BalI TGGCCA 2 cut(s) 2388, 2597
BamHI GGATCC 3 cut(s) 982, 2084, 2784
BanII GRGCYC 2 cut(s) 1473, 2061
BauI CACGAG 2 cut(s) 165, 1284
BbsI GAAGAC 3 cut(s) 812, 1387, 2069
Bbv12I GWGCWC 4 cut(s) 1473, 1548, 2061, 2863
BbvCI CCTCAGC 4 cut(s) 2133, 2467, 2570, 2658
BbvI GCAGC 6 cut(s) 218, 886, 1340, 2125, 2458, 2892
BccI CCATC 8 cut(s) 598, 836, 1135, 1672, 1790, 2298, 2698, 2775
BciT130I CCWGG 1 cut(s) 20
BclI TGATCA 1 cut(s) 1288
BcnI CCSGG 1 cut(s) 2301
BcoDI GTCTC 4 cut(s) 93, 561, 2483, 2559
BcuI ACTAGT 1 cut(s) 236
BfaI CTAG 5 cut(s) 237, 416, 750, 839, 1067
BfmI CTRYAG 2 cut(s) 459, 1181
BglII AGATCT 1 cut(s) 226
BisI GCNGC 6 cut(s) 207, 900, 1329, 2139, 2472, 2881
BlsI GCNGC 6 cut(s) 208, 901, 1330, 2140, 2473, 2882
BmcAI AGTACT 1 cut(s) 2957
Bme1390I CCNGG 2 cut(s) 20, 2301
Bme18I GGWCC 2 cut(s) 1210, 1315
BmgT120I GGNCC 2 cut(s) 1210, 1315
BmiI GGNNCC 4 cut(s) 984, 2086, 2464, 2786
BmrFI CCNGG 2 cut(s) 20, 2301
BmrI ACTGGG 1 cut(s) 120
BmsI GCATC 7 cut(s) 23, 204, 238, 372, 640, 1141, 2736
BmuI ACTGGG 1 cut(s) 120
BoxI GACNNNNGTC 1 cut(s) 1990
BpiI GAAGAC 3 cut(s) 812, 1387, 2069
BpmI CTGGAG 1 cut(s) 2847
Bpu10I CCTNAGC 5 cut(s) 1920, 2133, 2467, 2570, 2658
Bpu14I TTCGAA 1 cut(s) 1076
BpuEI CTTGAG 7 cut(s) 758, 1774, 1892, 2045, 2126, 2354, 2387
BpuMI CCSGG 1 cut(s) 2301
BsaAI YACGTR 1 cut(s) 2540
BsaBI GATNNNNATC 1 cut(s) 873
BsaI GGTCTC 1 cut(s) 2559
BsaJI CCNNGG 5 cut(s) 18, 432, 1097, 1351, 1673
BsaWI WCCGGW 3 cut(s) 444, 1207, 2830
Bsc4I CCNNNNNNNGG 7 cut(s) 433, 1324, 1436, 2252, 2384, 2395, 2681
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 7 cut(s) 115, 225, 288, 1679, 2213, 2531, 2953
Bse3DI GCAATG 2 cut(s) 190, 1646
Bse8I GATNNNNATC 1 cut(s) 873
BseBI CCWGG 1 cut(s) 20
BseDI CCNNGG 5 cut(s) 18, 432, 1097, 1351, 1673
BseJI GATNNNNATC 1 cut(s) 873
BseLI CCNNNNNNNGG 7 cut(s) 433, 1324, 1436, 2252, 2384, 2395, 2681
BseMI GCAATG 2 cut(s) 190, 1646
BseNI ACTGG 7 cut(s) 115, 225, 288, 1679, 2213, 2531, 2953
BseRI GAGGAG 1 cut(s) 2069
BseSI GKGCMC 1 cut(s) 1548
BseXI GCAGC 6 cut(s) 218, 886, 1340, 2125, 2458, 2892
BseYI CCCAGC 2 cut(s) 608, 2252
BsgI GTGCAG 1 cut(s) 2781
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 4 cut(s) 1065, 1678, 2388, 2597
BsiEI CGRYCG 1 cut(s) 33
BsiHKAI GWGCWC 4 cut(s) 1473, 1548, 2061, 2863
BsiSI CCGG 7 cut(s) 33, 445, 1208, 2301, 2396, 2513, 2831
BslFI GGGAC 5 cut(s) 294, 1333, 1645, 1706, 2947
BslI CCNNNNNNNGG 7 cut(s) 433, 1324, 1436, 2252, 2384, 2395, 2681
BsmAI GTCTC 4 cut(s) 93, 561, 2483, 2559
BsmBI CGTCTC 1 cut(s) 2483
BsmFI GGGAC 5 cut(s) 294, 1333, 1645, 1706, 2947
BsmI GAATGC 1 cut(s) 2194
BsnI GGCC 4 cut(s) 1065, 1678, 2388, 2597
Bso31I GGTCTC 1 cut(s) 2559
Bsp119I TTCGAA 1 cut(s) 1076
Bsp1286I GDGCHC 4 cut(s) 1473, 1548, 2061, 2863
Bsp1407I TGTACA 1 cut(s) 714
Bsp19I CCATGG 1 cut(s) 1097
BspANI GGCC 4 cut(s) 1065, 1678, 2388, 2597
BspHI TCATGA 2 cut(s) 1477, 1726
BspLI GGNNCC 4 cut(s) 984, 2086, 2464, 2786
BspMAI CTGCAG 1 cut(s) 1185
BspPI GGATC 8 cut(s) 337, 882, 977, 990, 2079, 2092, 2779, 2792
BspQI GCTCTTC 1 cut(s) 2933
BspT104I TTCGAA 1 cut(s) 1076
BspTNI GGTCTC 1 cut(s) 2559
BsrDI GCAATG 2 cut(s) 190, 1646
BsrFI RCCGGY 1 cut(s) 32
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 7 cut(s) 115, 225, 288, 1679, 2213, 2531, 2953
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 5 cut(s) 18, 432, 1097, 1351, 1673
BssSI CACGAG 2 cut(s) 165, 1284
BssT1I CCWWGG 2 cut(s) 1097, 1351
Bst2BI CACGAG 2 cut(s) 165, 1284
Bst2UI CCWGG 1 cut(s) 20
Bst4CI ACNGT 5 cut(s) 320, 366, 1386, 2285, 2742
Bst6I CTCTTC 4 cut(s) 134, 561, 815, 2933
BstAUI TGTACA 1 cut(s) 714
BstBAI YACGTR 1 cut(s) 2540
BstBI TTCGAA 1 cut(s) 1076
BstC8I GCNNGC 3 cut(s) 1569, 2130, 2330
BstDSI CCRYGG 2 cut(s) 432, 1097
BstHHI GCGC 1 cut(s) 2360
BstMAI GTCTC 4 cut(s) 93, 561, 2483, 2559
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 4 cut(s) 11, 1568, 2138, 2318
BstNI CCWGG 1 cut(s) 20
BstNSI RCATGY 6 cut(s) 106, 1571, 2252, 2512, 2554, 2875
BstPAI GACNNNNGTC 1 cut(s) 1990
BstSCI CCNGG 2 cut(s) 18, 2299
BstSFI CTRYAG 2 cut(s) 459, 1181
BstSLI GKGCMC 1 cut(s) 1548
BstV1I GCAGC 6 cut(s) 218, 886, 1340, 2125, 2458, 2892
BstV2I GAAGAC 3 cut(s) 812, 1387, 2069
BstX2I RGATCY 4 cut(s) 226, 982, 2084, 2784
BstYI RGATCY 4 cut(s) 226, 982, 2084, 2784
BsuRI GGCC 4 cut(s) 1065, 1678, 2388, 2597
BtgI CCRYGG 2 cut(s) 432, 1097
BtsIMutI CAGTG 1 cut(s) 108
Cac8I GCNNGC 3 cut(s) 1569, 2130, 2330
CciI TCATGA 2 cut(s) 1477, 1726
CfoI GCGC 1 cut(s) 2360
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 1210, 1315
CseI GACGC 2 cut(s) 1560, 2500
Csp6I GTAC 7 cut(s) 23, 332, 483, 715, 836, 2950, 2956
CviQI GTAC 7 cut(s) 23, 332, 483, 715, 836, 2950, 2956
DraI TTTAAA 1 cut(s) 2023
DraIII CACNNNGTG 1 cut(s) 2504
EaeI YGGCCR 2 cut(s) 2386, 2595
Eam1104I CTCTTC 4 cut(s) 134, 561, 815, 2933
EarI CTCTTC 4 cut(s) 134, 561, 815, 2933
Ecl136II GAGCTC 2 cut(s) 1471, 2059
Eco130I CCWWGG 2 cut(s) 1097, 1351
Eco24I GRGCYC 2 cut(s) 1473, 2061
Eco31I GGTCTC 1 cut(s) 2559
Eco32I GATATC 1 cut(s) 2045
Eco47I GGWCC 2 cut(s) 1210, 1315
Eco53kI GAGCTC 2 cut(s) 1471, 2059
Eco57I CTGAAG 2 cut(s) 38, 273
EcoICRI GAGCTC 2 cut(s) 1471, 2059
EcoRI GAATTC 1 cut(s) 1129
EcoRII CCWGG 1 cut(s) 18
EcoRV GATATC 1 cut(s) 2045
EcoT14I CCWWGG 2 cut(s) 1097, 1351
EcoT22I ATGCAT 2 cut(s) 1467, 2020
EcoT38I GRGCYC 2 cut(s) 1473, 2061
ErhI CCWWGG 2 cut(s) 1097, 1351
Esp3I CGTCTC 1 cut(s) 2483
FalI AAGNNNNNCTT 6 cut(s) 280, 312, 2147, 2179, 2214, 2246
FaqI GGGAC 5 cut(s) 294, 1333, 1645, 1706, 2947
FbaI TGATCA 1 cut(s) 1288
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 6 cut(s) 207, 900, 1329, 2139, 2472, 2881
FriOI GRGCYC 2 cut(s) 1473, 2061
Fsp4HI GCNGC 6 cut(s) 207, 900, 1329, 2139, 2472, 2881
FspBI CTAG 5 cut(s) 237, 416, 750, 839, 1067
FspI TGCGCA 1 cut(s) 2359
GlaI GCGC 1 cut(s) 2359
GluI GCNGC 6 cut(s) 207, 900, 1329, 2139, 2472, 2881
GsaI CCCAGC 2 cut(s) 612, 2256
GsuI CTGGAG 1 cut(s) 2847
HaeIII GGCC 4 cut(s) 1065, 1678, 2388, 2597
HapII CCGG 7 cut(s) 33, 445, 1208, 2301, 2396, 2513, 2831
HgaI GACGC 2 cut(s) 1560, 2500
HhaI GCGC 1 cut(s) 2360
Hin6I GCGC 1 cut(s) 2358
HinP1I GCGC 1 cut(s) 2358
HincII GTYRAC 5 cut(s) 67, 118, 316, 1995, 2898
HindII GTYRAC 5 cut(s) 67, 118, 316, 1995, 2898
HindIII AAGCTT 3 cut(s) 1750, 1922, 2330
HpaII CCGG 7 cut(s) 33, 445, 1208, 2301, 2396, 2513, 2831
HphI GGTGA 2 cut(s) 1454, 2824
Hpy166II GTNNAC 8 cut(s) 67, 108, 118, 316, 1546, 1995, 2369, 2898
Hpy188I TCNGA 9 cut(s) 131, 370, 792, 1674, 2049, 2198, 2564, 2780, 2856
Hpy8I GTNNAC 8 cut(s) 67, 108, 118, 316, 1546, 1995, 2369, 2898
Hpy99I CGWCG 2 cut(s) 46, 122
HpyCH4III ACNGT 5 cut(s) 320, 366, 1386, 2285, 2742
HpyCH4IV ACGT 2 cut(s) 41, 2539
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 1568, 2138, 2318
HpySE526I ACGT 2 cut(s) 41, 2539
HspAI GCGC 1 cut(s) 2358
Ksp22I TGATCA 1 cut(s) 1288
LguI GCTCTTC 1 cut(s) 2933
LmnI GCTCC 5 cut(s) 481, 912, 2056, 2176, 2866
Lsp1109I GCAGC 6 cut(s) 218, 886, 1340, 2125, 2458, 2892
LweI GCATC 7 cut(s) 23, 204, 238, 372, 640, 1141, 2736
MaeI CTAG 5 cut(s) 237, 416, 750, 839, 1067
MaeII ACGT 2 cut(s) 41, 2539
MaeIII GTNAC 9 cut(s) 406, 992, 1826, 2145, 2265, 2279, 2504, 2522, 2540
MflI RGATCY 4 cut(s) 226, 982, 2084, 2784
MhlI GDGCHC 4 cut(s) 1473, 1548, 2061, 2863
MlsI TGGCCA 2 cut(s) 2388, 2597
MluNI TGGCCA 2 cut(s) 2388, 2597
MlyI GAGTC 4 cut(s) 81, 334, 749, 1780
MmeI TCCRAC 4 cut(s) 302, 1342, 1357, 2154
Mox20I TGGCCA 2 cut(s) 2388, 2597
Mph1103I ATGCAT 2 cut(s) 1467, 2020
MroXI GAANNNNTTC 4 cut(s) 580, 760, 2010, 2373
MscI TGGCCA 2 cut(s) 2388, 2597
MseI TTAA 7 cut(s) 375, 1035, 1160, 1946, 1959, 2022, 2241
MslI CAYNNNNRTG 1 cut(s) 2632
Msp20I TGGCCA 2 cut(s) 2388, 2597
MspA1I CMGCKG 1 cut(s) 2471
MspI CCGG 7 cut(s) 33, 445, 1208, 2301, 2396, 2513, 2831
MspR9I CCNGG 2 cut(s) 20, 2301
Mva1269I GAATGC 1 cut(s) 2194
MvaI CCWGG 1 cut(s) 20
MwoI GCNNNNNNNGC 4 cut(s) 11, 1568, 2138, 2318
NciI CCSGG 1 cut(s) 2301
NcoI CCATGG 1 cut(s) 1097
NlaIV GGNNCC 4 cut(s) 984, 2086, 2464, 2786
NmeAIII GCCGAG 1 cut(s) 1543
NmuCI GTSAC 3 cut(s) 992, 2504, 2540
NsbI TGCGCA 1 cut(s) 2359
NsiI ATGCAT 2 cut(s) 1467, 2020
NspI RCATGY 6 cut(s) 106, 1571, 2252, 2512, 2554, 2875
NspV TTCGAA 1 cut(s) 1076
PaeI GCATGC 1 cut(s) 1571
PagI TCATGA 2 cut(s) 1477, 1726
PciI ACATGT 2 cut(s) 102, 2871
PciSI GCTCTTC 1 cut(s) 2933
PctI GAATGC 1 cut(s) 2194
PdmI GAANNNNTTC 4 cut(s) 580, 760, 2010, 2373
PfeI GAWTC 8 cut(s) 157, 500, 1030, 1799, 2101, 2120, 2579, 2815
PflFI GACNNNGTC 1 cut(s) 71
PflMI CCANNNNNTGG 2 cut(s) 1436, 2252
PfoI TCCNGGA 1 cut(s) 2299
PkrI GCNGC 6 cut(s) 208, 901, 1330, 2140, 2473, 2882
PleI GAGTC 4 cut(s) 80, 333, 748, 1779
PpsI GAGTC 4 cut(s) 80, 333, 748, 1779
Ppu21I YACGTR 1 cut(s) 2540
PscI ACATGT 2 cut(s) 102, 2871
PshAI GACNNNNGTC 1 cut(s) 1990
Psp124BI GAGCTC 2 cut(s) 1473, 2061
Psp6I CCWGG 1 cut(s) 18
PspFI CCCAGC 2 cut(s) 608, 2252
PspGI CCWGG 1 cut(s) 18
PspN4I GGNNCC 4 cut(s) 984, 2086, 2464, 2786
PspPI GGNCC 2 cut(s) 1210, 1315
PstI CTGCAG 1 cut(s) 1185
PsuI RGATCY 4 cut(s) 226, 982, 2084, 2784
PsyI GACNNNGTC 1 cut(s) 71
PvuII CAGCTG 1 cut(s) 2471
RsaI GTAC 7 cut(s) 24, 333, 484, 716, 837, 2951, 2957
RsaNI GTAC 7 cut(s) 23, 332, 483, 715, 836, 2950, 2956
RseI CAYNNNNRTG 1 cut(s) 2632
SacI GAGCTC 2 cut(s) 1473, 2061
SalI GTCGAC 1 cut(s) 116
SapI GCTCTTC 1 cut(s) 2933
SaqAI TTAA 7 cut(s) 375, 1035, 1160, 1946, 1959, 2022, 2241
SatI GCNGC 6 cut(s) 207, 900, 1329, 2139, 2472, 2881
Sau96I GGNCC 2 cut(s) 1210, 1315
ScaI AGTACT 1 cut(s) 2957
SchI GAGTC 4 cut(s) 81, 334, 749, 1780
ScrFI CCNGG 2 cut(s) 20, 2301
SduI GDGCHC 4 cut(s) 1473, 1548, 2061, 2863
SfaNI GCATC 7 cut(s) 23, 204, 238, 372, 640, 1141, 2736
SfcI CTRYAG 2 cut(s) 459, 1181
SfuI TTCGAA 1 cut(s) 1076
SinI GGWCC 2 cut(s) 1210, 1315
SmiMI CAYNNNNRTG 1 cut(s) 2632
SmlI CTYRAG 7 cut(s) 737, 1753, 1907, 2060, 2105, 2333, 2402
SmoI CTYRAG 7 cut(s) 737, 1753, 1907, 2060, 2105, 2333, 2402
SpeI ACTAGT 1 cut(s) 236
SphI GCATGC 1 cut(s) 1571
SspI AATATT 1 cut(s) 1435
SspMI CTAG 5 cut(s) 237, 416, 750, 839, 1067
SstI GAGCTC 2 cut(s) 1473, 2061
StyD4I CCNGG 2 cut(s) 18, 2299
StyI CCWWGG 2 cut(s) 1097, 1351
TaaI ACNGT 5 cut(s) 320, 366, 1386, 2285, 2742
TaiI ACGT 2 cut(s) 44, 2542
TaqI TCGA 6 cut(s) 29, 117, 934, 1076, 1406, 2004
TatI WGTACW 4 cut(s) 482, 714, 835, 2955
TfiI GAWTC 8 cut(s) 157, 500, 1030, 1799, 2101, 2120, 2579, 2815
Tru1I TTAA 7 cut(s) 375, 1035, 1160, 1946, 1959, 2022, 2241
Tru9I TTAA 7 cut(s) 375, 1035, 1160, 1946, 1959, 2022, 2241
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 3 cut(s) 992, 2504, 2540
TseI GCWGC 6 cut(s) 206, 899, 1328, 2138, 2471, 2880
Tsp45I GTSAC 3 cut(s) 992, 2504, 2540
TspDTI ATGAA 9 cut(s) 167, 513, 554, 1466, 1743, 1919, 2003, 2598, 2603
TspGWI ACGGA 2 cut(s) 215, 449
TspRI CASTG 1 cut(s) 115
Tth111I GACNNNGTC 1 cut(s) 71
Van91I CCANNNNNTGG 2 cut(s) 1436, 2252
VneI GTGCAC 1 cut(s) 1544
VpaK11BI GGWCC 2 cut(s) 1210, 1315
XapI RAATTY 7 cut(s) 80, 668, 942, 1129, 1730, 1765, 1810
XceI RCATGY 6 cut(s) 106, 1571, 2252, 2512, 2554, 2875
XmiI GTMKAC 1 cut(s) 117
XmnI GAANNNNTTC 4 cut(s) 580, 760, 2010, 2373
XspI CTAG 5 cut(s) 237, 416, 750, 839, 1067
ZrmI AGTACT 1 cut(s) 2957
Zsp2I ATGCAT 2 cut(s) 1467, 2020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.