Rroxscaffold_2G00103020

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001339
Physical Location & Seq
Reverse (-)
25442126 .. 25445831
3706 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00103020.1

Sequence Viewer

Length: 1701 bp
ATGGCGACGACTATAGCGTCTCTAGTACTTTCCACCGGCATCACTTCGGCAGTTCTTCAGGTTCTTCTCGACAGAATTGCCAATTTCACTCAAACAAAGATAACCCAGTTTGCCCAGCTGCACGTCGAGTTCTTGAAGTTGGGAGCAACAATGTCCAAGGCTAAAGCTCTTGTTGATGATGTAGAGAACAAGAAATGTTTTCCAGAGGCTTGCCGAATTTTGCTCGAAGATGTCAAAAGTGTTGTTTTAGATGCCGATGATTTGCTGGATGAGATTGACATGGACCTCGAAAATAGGAATTCTACCGACTTCAACAACAATCAGGTTAGTCAAATGTTCCTATCATCTCGTAGATTCAATATTCCTTCTGAGCTAAGCAAGATCCATAAAGAACTGGAGGGTTTAGTAGAGAAACTGGAGGGCTACTGTATGATAAATTCGGGTCAACCTCAAATGGCTGCTCCTGTTATTCCCAGTACTTCTTTAGTGGATGAGTCATCTATAATTGCAAGAGTTTCAGACAAGGAAAAGATCATTCAGATGGTCTTGTCTGACCAAGGAGAGGATGGAGGAAATGTTTCTGTGATTCCAATAGTGGGAATGGGCGGAATTGGGAAAACAGCACTAGCTCAACTTGTTTACAATGATCCAGTTGTGGCTAAAAATTTCGATTTTAAAATCTGGGTATCTGTTTCTTTGAGCTGTGATGTTGTCAAGATTACAAAATCCATTTTTCACTCTCTTACTGAGCAAGTGTCTGGGTTGTCTGAACTCAAGAGAGCTTTTGAGTTATCTGATCTAAGCAAATATTTTGAGTTGAGTCTTAATGAGATTCATATTCAGTTACAAAAGAAGTTGAGGGGGAAGAAGTTTTTGCTTGTACTTGACGATATGTGGAATGAGAATCCTAGAGATTGGGATGTATTGAATTTGCCTTTTCAAGTTGCAGCACAAGGAAGCAAGATTGTGATGACTACCCGAAGCAAAATTGCTTCGTCTATTCTTTCTGGTGCTTCTGCTTATCATTTGGATACTTTGTCTCATAATGATTGCTGGACAATCATTAAACAGACGGCAGGACTAAGTGATGCACATGAAAATGAATTGATTGGGTTAAAGATAGCTAAGAAGTGCAAAGGGCTTCCTTTGGCGGCAAAAGTGATTGGAAGTGTCTTACGTTATAAATCTGGAGACAGGAAGTGGGATGCTTTGTTAAAATGTGAGCTTTGGGAATCTCCAGAACATGGAAACCATATCTATCCAGCACTTAAGTTGAGCTATGATAATTTGTCTTCTTATTTGAGAAGATGTTTCACTTATTGTTCTATCTTCCCACGTGATCATGAATTTGAAAAAGATGATTTGGTTCAGTTATGGGCAGCAGAAGGCTTCATTCAAGCTCAAGGAAGAGAAGCAATTGAAGACGTTGGAAGAGAGTACTTTGTTGGGTTGTGTTCGAGGTCTTTTTTCCAAGTTTCAGATGATATTCAACCAAGATATAAAATGCATGACCTCATTCATGATTTGGCGAGATTGATTTCAGCCAACATTTCCTTTTCTATGGAGGATAATATCTCAACCTTCTTGCCCATACCTAGACATGCTCGCTATTCATCATTGCTTTGTCAAGACATTAAGCCATCTACATTGGAGGTGTTTCACAGGTATCAGAAGTTGCGAACCTTTATGCTGCTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

566

Amino Acids

63.73

Weight (kDa)

5.92

Isoelectric Point (pI)

34.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 18 - 104 1.2e-09 Rx N-terminal domain
NB-ARC PF00931 176 - 360 1.3e-31 NB-ARC domain
WHD_DRP PF23559 443 - 510 5.4e-27 Disease resistance protein Winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1182
AasI GACNNNNNNGTC 1 cut(s) 16
AccB7I CCANNNNNTGG 1 cut(s) 1244
AciI CCGC 2 cut(s) 606, 1151
AclWI GGATC 2 cut(s) 376, 641
AcsI RAATTY 6 cut(s) 216, 298, 436, 664, 928, 1346
AcuI CTGAAG 1 cut(s) 41
AcvI CACGTG 1 cut(s) 1337
AfaI GTAC 4 cut(s) 27, 478, 882, 1439
AfiI CCNNNNNNNGG 3 cut(s) 562, 596, 1244
AflII CTTAAG 1 cut(s) 1268
AgsI TTSAA 9 cut(s) 136, 313, 358, 928, 941, 1352, 1397, 1421, 1490
AjiI CACGTC 1 cut(s) 124
Alw26I GTCTC 3 cut(s) 24, 1044, 1185
AlwI GGATC 2 cut(s) 376, 641
ApeKI GCWGC 5 cut(s) 118, 458, 947, 1379, 1690
ApoI RAATTY 6 cut(s) 216, 298, 436, 664, 928, 1346
Asp700I GAANNNNTTC 1 cut(s) 577
AspS9I GGNCC 1 cut(s) 283
AvaII GGWCC 1 cut(s) 283
BbrPI CACGTG 1 cut(s) 1337
BbsI GAAGAC 2 cut(s) 1284, 1428
BbvI GCAGC 5 cut(s) 105, 445, 959, 1391, 1677
BccI CCATC 3 cut(s) 535, 560, 1648
BceAI ACGGC 1 cut(s) 1089
BcgI CGANNNNNNTGC 2 cut(s) 59, 93
BciVI GTATCC 1 cut(s) 1024
BclI TGATCA 1 cut(s) 1339
BcoDI GTCTC 3 cut(s) 24, 1044, 1185
BfaI CTAG 4 cut(s) 23, 626, 909, 1596
BfmI CTRYAG 1 cut(s) 12
BfrI CTTAAG 1 cut(s) 1268
BfuI GTATCC 1 cut(s) 1024
BisI GCNGC 6 cut(s) 119, 459, 948, 1152, 1380, 1691
BlpI GCTNAGC 1 cut(s) 374
BlsI GCNGC 6 cut(s) 120, 460, 949, 1153, 1381, 1692
BmcAI AGTACT 3 cut(s) 27, 478, 1439
Bme18I GGWCC 1 cut(s) 283
BmgBI CACGTC 1 cut(s) 124
BmgT120I GGNCC 1 cut(s) 283
BmrI ACTGGG 2 cut(s) 100, 468
BmsI GCATC 4 cut(s) 48, 241, 1078, 1195
BmuI ACTGGG 2 cut(s) 100, 468
BpiI GAAGAC 2 cut(s) 1284, 1428
BpmI CTGGAG 4 cut(s) 416, 437, 1209, 1221
Bpu1102I GCTNAGC 1 cut(s) 374
BpuEI CTTGAG 2 cut(s) 758, 1386
BsaAI YACGTR 1 cut(s) 1337
BsaJI CCNNGG 2 cut(s) 156, 556
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 3 cut(s) 562, 596, 1244
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 5 cut(s) 106, 399, 420, 474, 650
Bse3DI GCAATG 1 cut(s) 1616
BseDI CCNNGG 2 cut(s) 156, 556
BseGI GGATG 5 cut(s) 274, 496, 571, 925, 1210
BseLI CCNNNNNNNGG 3 cut(s) 562, 596, 1244
BseMI GCAATG 1 cut(s) 1616
BseMII CTCAG 2 cut(s) 360, 738
BseNI ACTGG 5 cut(s) 106, 399, 420, 474, 650
BseXI GCAGC 5 cut(s) 105, 445, 959, 1391, 1677
BseYI CCCAGC 1 cut(s) 114
BsgI GTGCAG 1 cut(s) 104
BsiSI CCGG 1 cut(s) 36
BslI CCNNNNNNNGG 3 cut(s) 562, 596, 1244
BsmAI GTCTC 3 cut(s) 24, 1044, 1185
BsmBI CGTCTC 1 cut(s) 24
Bsp143I GATC 5 cut(s) 381, 531, 646, 796, 1339
Bsp1720I GCTNAGC 1 cut(s) 374
BspACI CCGC 2 cut(s) 606, 1151
BspCNI CTCAG 2 cut(s) 361, 739
BspHI TCATGA 2 cut(s) 1342, 1519
BspPI GGATC 2 cut(s) 376, 641
BspTI CTTAAG 1 cut(s) 1268
BsrDI GCAATG 1 cut(s) 1616
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 5 cut(s) 106, 399, 420, 474, 650
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 2 cut(s) 156, 556
BssMI GATC 5 cut(s) 381, 531, 646, 796, 1339
BssT1I CCWWGG 2 cut(s) 156, 556
Bst4CI ACNGT 1 cut(s) 428
Bst6I CTCTTC 2 cut(s) 1402, 1426
BstAFI CTTAAG 1 cut(s) 1268
BstBAI YACGTR 1 cut(s) 1337
BstC8I GCNNGC 2 cut(s) 211, 1606
BstDEI CTNAG 6 cut(s) 369, 374, 747, 800, 1082, 1125
BstF5I GGATG 5 cut(s) 274, 496, 571, 925, 1210
BstKTI GATC 5 cut(s) 384, 534, 649, 799, 1342
BstMAI GTCTC 3 cut(s) 24, 1044, 1185
BstMBI GATC 5 cut(s) 381, 531, 646, 796, 1339
BstNSI RCATGY 1 cut(s) 1604
BstSFI CTRYAG 1 cut(s) 12
BstV1I GCAGC 5 cut(s) 105, 445, 959, 1391, 1677
BstV2I GAAGAC 2 cut(s) 1284, 1428
BstX2I RGATCY 1 cut(s) 381
BstYI RGATCY 1 cut(s) 381
BsuI GTATCC 1 cut(s) 1024
BtrI CACGTC 1 cut(s) 124
BtsCI GGATG 5 cut(s) 274, 496, 571, 925, 1210
Cac8I GCNNGC 2 cut(s) 211, 1606
CciI TCATGA 2 cut(s) 1342, 1519
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 283
CseI GACGC 1 cut(s) 6
Csp6I GTAC 4 cut(s) 26, 477, 881, 1438
CviAII CATG 7 cut(s) 280, 1094, 1244, 1343, 1508, 1520, 1601
CviQI GTAC 4 cut(s) 26, 477, 881, 1438
DdeI CTNAG 6 cut(s) 369, 374, 747, 800, 1082, 1125
DpnI GATC 5 cut(s) 383, 533, 648, 798, 1341
DpnII GATC 5 cut(s) 381, 531, 646, 796, 1339
DraI TTTAAA 1 cut(s) 676
DrdI GACNNNNNNGTC 1 cut(s) 16
DseDI GACNNNNNNGTC 1 cut(s) 16
Eam1104I CTCTTC 2 cut(s) 1402, 1426
EarI CTCTTC 2 cut(s) 1402, 1426
EciI GGCGGA 1 cut(s) 621
Eco130I CCWWGG 2 cut(s) 156, 556
Eco47I GGWCC 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 41
Eco72I CACGTG 1 cut(s) 1337
EcoRI GAATTC 1 cut(s) 298
EcoT14I CCWWGG 2 cut(s) 156, 556
EcoT22I ATGCAT 1 cut(s) 1509
ErhI CCWWGG 2 cut(s) 156, 556
Esp3I CGTCTC 1 cut(s) 24
FaeI CATG 7 cut(s) 283, 1097, 1247, 1346, 1511, 1523, 1604
FatI CATG 7 cut(s) 279, 1093, 1243, 1342, 1507, 1519, 1600
FbaI TGATCA 1 cut(s) 1339
Fnu4HI GCNGC 6 cut(s) 119, 459, 948, 1152, 1380, 1691
FokI GGATG 5 cut(s) 281, 503, 578, 932, 1217
Fsp4HI GCNGC 6 cut(s) 119, 459, 948, 1152, 1380, 1691
FspBI CTAG 4 cut(s) 23, 626, 909, 1596
GluI GCNGC 6 cut(s) 119, 459, 948, 1152, 1380, 1691
GsaI CCCAGC 1 cut(s) 118
GsuI CTGGAG 4 cut(s) 416, 437, 1209, 1221
HapII CCGG 1 cut(s) 36
HgaI GACGC 1 cut(s) 6
Hin1II CATG 7 cut(s) 283, 1097, 1247, 1346, 1511, 1523, 1604
HincII GTYRAC 1 cut(s) 446
HindII GTYRAC 1 cut(s) 446
HinfI GANTC 7 cut(s) 354, 494, 586, 820, 832, 904, 1232
HpaII CCGG 1 cut(s) 36
Hpy166II GTNNAC 2 cut(s) 446, 640
Hpy188I TCNGA 9 cut(s) 370, 520, 540, 553, 769, 796, 1480, 1671, 1700
Hpy8I GTNNAC 2 cut(s) 446, 640
Hpy99I CGWCG 2 cut(s) 10, 128
HpyAV CCTTC 3 cut(s) 375, 1379, 1591
HpyCH4III ACNGT 1 cut(s) 428
HpyCH4IV ACGT 4 cut(s) 123, 1177, 1336, 1425
HpyCH4V TGCA 6 cut(s) 121, 509, 947, 1091, 1134, 1507
HpyF3I CTNAG 6 cut(s) 369, 374, 747, 800, 1082, 1125
HpySE526I ACGT 4 cut(s) 123, 1177, 1336, 1425
Hsp92II CATG 7 cut(s) 283, 1097, 1247, 1346, 1511, 1523, 1604
Ksp22I TGATCA 1 cut(s) 1339
Kzo9I GATC 5 cut(s) 381, 531, 646, 796, 1339
LmnI GCTCC 2 cut(s) 143, 466
Lsp1109I GCAGC 5 cut(s) 105, 445, 959, 1391, 1677
LweI GCATC 4 cut(s) 48, 241, 1078, 1195
MaeI CTAG 4 cut(s) 23, 626, 909, 1596
MaeII ACGT 4 cut(s) 123, 1177, 1336, 1425
MaeIII GTNAC 1 cut(s) 843
MalI GATC 5 cut(s) 383, 533, 648, 798, 1341
MboI GATC 5 cut(s) 381, 531, 646, 796, 1339
MfeI CAATTG 1 cut(s) 1416
MflI RGATCY 1 cut(s) 381
MlyI GAGTC 2 cut(s) 503, 829
MmeI TCCRAC 1 cut(s) 1408
Mph1103I ATGCAT 1 cut(s) 1509
MroXI GAANNNNTTC 1 cut(s) 577
MseI TTAA 7 cut(s) 675, 825, 1065, 1115, 1214, 1269, 1635
MslI CAYNNNNRTG 2 cut(s) 539, 1098
MspA1I CMGCKG 1 cut(s) 118
MspCI CTTAAG 1 cut(s) 1268
MspI CCGG 1 cut(s) 36
MunI CAATTG 1 cut(s) 1416
NdeII GATC 5 cut(s) 381, 531, 646, 796, 1339
NlaIII CATG 7 cut(s) 283, 1097, 1247, 1346, 1511, 1523, 1604
NsiI ATGCAT 1 cut(s) 1509
NspI RCATGY 1 cut(s) 1604
PagI TCATGA 2 cut(s) 1342, 1519
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PdmI GAANNNNTTC 1 cut(s) 577
PfeI GAWTC 5 cut(s) 354, 586, 832, 904, 1232
PflMI CCANNNNNTGG 1 cut(s) 1244
PkrI GCNGC 6 cut(s) 120, 460, 949, 1153, 1381, 1692
PleI GAGTC 2 cut(s) 502, 828
PmaCI CACGTG 1 cut(s) 1337
PmlI CACGTG 1 cut(s) 1337
PpsI GAGTC 2 cut(s) 502, 828
Ppu21I YACGTR 1 cut(s) 1337
PsiI TTATAA 1 cut(s) 1182
PspCI CACGTG 1 cut(s) 1337
PspFI CCCAGC 1 cut(s) 114
PspPI GGNCC 1 cut(s) 283
PsuI RGATCY 1 cut(s) 381
PvuII CAGCTG 1 cut(s) 118
RsaI GTAC 4 cut(s) 27, 478, 882, 1439
RsaNI GTAC 4 cut(s) 26, 477, 881, 1438
RseI CAYNNNNRTG 2 cut(s) 539, 1098
SaqAI TTAA 7 cut(s) 675, 825, 1065, 1115, 1214, 1269, 1635
SatI GCNGC 6 cut(s) 119, 459, 948, 1152, 1380, 1691
Sau3AI GATC 5 cut(s) 381, 531, 646, 796, 1339
Sau96I GGNCC 1 cut(s) 283
ScaI AGTACT 3 cut(s) 27, 478, 1439
SchI GAGTC 2 cut(s) 503, 829
SfaNI GCATC 4 cut(s) 48, 241, 1078, 1195
SfcI CTRYAG 1 cut(s) 12
SinI GGWCC 1 cut(s) 283
SmiMI CAYNNNNRTG 2 cut(s) 539, 1098
SmlI CTYRAG 3 cut(s) 773, 1268, 1401
SmoI CTYRAG 3 cut(s) 773, 1268, 1401
SsiI CCGC 2 cut(s) 606, 1151
SspI AATATT 2 cut(s) 361, 809
SspMI CTAG 4 cut(s) 23, 626, 909, 1596
StyI CCWWGG 2 cut(s) 156, 556
TaaI ACNGT 1 cut(s) 428
TaiI ACGT 4 cut(s) 126, 1180, 1339, 1428
TaqI TCGA 6 cut(s) 69, 126, 225, 288, 669, 1457
TatI WGTACW 4 cut(s) 25, 476, 880, 1437
TauI GCSGC 1 cut(s) 1154
TfiI GAWTC 5 cut(s) 354, 586, 832, 904, 1232
Tru1I TTAA 7 cut(s) 675, 825, 1065, 1115, 1214, 1269, 1635
Tru9I TTAA 7 cut(s) 675, 825, 1065, 1115, 1214, 1269, 1635
TseI GCWGC 5 cut(s) 118, 458, 947, 1379, 1690
TspDTI ATGAA 7 cut(s) 824, 1110, 1116, 1359, 1381, 1508, 1602
Van91I CCANNNNNTGG 1 cut(s) 1244
Vha464I CTTAAG 1 cut(s) 1268
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 6 cut(s) 216, 298, 436, 664, 928, 1346
XceI RCATGY 1 cut(s) 1604
XcmI CCANNNNNNNNNTGG 1 cut(s) 563
XmnI GAANNNNTTC 1 cut(s) 577
XspI CTAG 4 cut(s) 23, 626, 909, 1596
ZrmI AGTACT 3 cut(s) 27, 478, 1439
Zsp2I ATGCAT 1 cut(s) 1509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.