Rh7CG465400

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0001339
Physical Location & Seq
Reverse (-)
62194937 .. 62196301
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG465400.1

Sequence Viewer

Length: 1365 bp
ATGCATGACCTCATTCATGATTTGGCGAGATTGGTTTCAGCCAACATTTCCTTTTCCATGGAGGATAATATCTCAACCATCCTGCCCATACCTAGACATGCTCGGTATTCTTCATTGCTTTGTCAAGACATTAAGCCATCTACATTGGAGGTGTTTCACAGGTATGAGAAGCTGCGAACCTTTATGCTGCTATCTGAACATGCATCCAATCTTTACCAGGTTCCATATGATTTCTTTATAAAGTTGGGACGCTTAAGAGTGTTGAATCTGAGTCATAGTGGCATCTCTGACTTGCCTGACTCTATTAAGAATTTGATACACCTTCGTTACCTAAATCTTAGTCATACCTCTATCAAAAGATTGCCGAAATCTATAGCAGATATATATGGGTTGGAAACACTTAAGCTCAAGAATTGTTGTGAGCTTCTTCAATTGCCTGACAACTTGAAGAACTTGATTAAGCTACGACATCTTGACTTTGATGGATATCGTTTAATCAGTTCCATGCCAATAGAAGTTGGGAAACTGACCAGTCTTGAAACTCTGCCTGCATTTATAGTGGGAAAGCACAAAGGATATCAAATTGGGGAGCTCAAGAACATGAGGTTCCTTCGTGGATTCATTTGCATCACAAATCTTGAGAATGTGGCAAGTTCTGTAGAGGGTGAGGCTGCTATGTTACACGAGAAGCAATACCTAGAGAAGTTGGAGTTAGAATGGAATGAAATGGGTGCTCAAACAGTTGAACAAGAAGTTCTTACGAGCCTTAAACCTCATGTTGGGTTGAGAGAGTTACGAGTAACAGGCTATTGTGGTTTAATATTTCCAAATTGGATTGCTGACTCATCATTTTGCAAGCTTGAGAGAATCGACATAGACAAGTGCCATTTTTGTACACTTCTTCCTTCTCTTGGTCAACTTCCGGCGCTAAAGTATCTTCTCATTCAAGATATGCGTGCTTTGGAAAATGTAGATCTTCTGTTGTGTGGGTCGGCAGGTAGTTTTCCGTCATTGGAGACGCTCACATTTCGAGACATGCCAAAGTTGAAGATTTGGTCAGGAATAAATGAAAATGGCATGCCTCACCTTAGAGTGCTAAGCATTGACTCTTGTCCAGAATTGGTCACACTACCTTCAACACATTACCTAAGATCACTTGAAATGTTAGAAATAAATCGTTGCCCTGAGCTTCAATCATTGCCAAGGGAAGGACTGCCACTCTCACTCACATGTCTGATAATTTCGGAAAGTGATATTTTAAAGGAGCGATGCGAGGTGCAAGGTGCAGATTGGGACAAGATCAAATCAGTCCCCAACATAATGATTGATGATGAAGTGATTCCCATGGAAGGACGTCAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

454

Amino Acids

51.73

Weight (kDa)

6.04

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 69 - 118 1.1e-06 Leucine rich repeat
LRR_14 PF23598 82 - 367 3.5e-33 Leucine-rich repeat region
LRR_8 PF13855 84 - 139 1.2e-07 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 195 - 318 1.6e-35 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 280 - 394 3.6e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 239
AatII GACGTC 1 cut(s) 1357
Acc36I ACCTGC 1 cut(s) 986
AcsI RAATTY 1 cut(s) 310
AcyI GRCGYC 1 cut(s) 1354
AfaI GTAC 1 cut(s) 895
AfiI CCNNNNNNNGG 4 cut(s) 779, 911, 1208, 1349
AflII CTTAAG 2 cut(s) 253, 401
AflIII ACRYGT 1 cut(s) 1229
AjnI CCWGG 1 cut(s) 216
AluBI AGCT 7 cut(s) 172, 406, 424, 463, 592, 859, 1189
AluI AGCT 7 cut(s) 172, 406, 424, 463, 592, 859, 1189
Alw21I GWGCWC 2 cut(s) 594, 736
Alw26I GTCTC 2 cut(s) 1010, 1026
ApeKI GCWGC 3 cut(s) 172, 187, 671
ApoI RAATTY 1 cut(s) 310
Asp700I GAANNNNTTC 1 cut(s) 1338
AspLEI GCGC 1 cut(s) 928
AsuHPI GGTGA 2 cut(s) 677, 1076
BanII GRGCYC 1 cut(s) 594
BauI CACGAG 1 cut(s) 683
Bbv12I GWGCWC 2 cut(s) 594, 736
BbvI GCAGC 3 cut(s) 159, 174, 658
BccI CCATC 3 cut(s) 86, 145, 476
BciT130I CCWGG 1 cut(s) 218
BcoDI GTCTC 2 cut(s) 1010, 1026
BfaI CTAG 2 cut(s) 93, 698
BfmI CTRYAG 2 cut(s) 372, 657
BfoI RGCGCY 1 cut(s) 929
BfrI CTTAAG 2 cut(s) 253, 401
BfuAI ACCTGC 1 cut(s) 986
BglII AGATCT 1 cut(s) 973
BisI GCNGC 3 cut(s) 173, 188, 672
BlpI GCTNAGC 1 cut(s) 1097
BlsI GCNGC 3 cut(s) 174, 189, 673
Bme1390I CCNGG 1 cut(s) 218
BmiI GGNNCC 2 cut(s) 222, 608
BmrFI CCNGG 1 cut(s) 218
BmsI GCATC 4 cut(s) 212, 291, 636, 1259
BoxI GACNNNNGTC 1 cut(s) 1110
Bpu10I CCTNAGC 1 cut(s) 1185
Bpu1102I GCTNAGC 1 cut(s) 1097
BpuEI CTTGAG 4 cut(s) 392, 578, 659, 881
BsaBI GATNNNNATC 1 cut(s) 486
BsaHI GRCGYC 1 cut(s) 1354
BsaJI CCNNGG 3 cut(s) 57, 1202, 1344
Bsc4I CCNNNNNNNGG 4 cut(s) 779, 911, 1208, 1349
Bse1I ACTGG 1 cut(s) 531
Bse3DI GCAATG 2 cut(s) 113, 1196
Bse8I GATNNNNATC 1 cut(s) 486
BseBI CCWGG 1 cut(s) 218
BseDI CCNNGG 3 cut(s) 57, 1202, 1344
BseGI GGATG 2 cut(s) 78, 203
BseJI GATNNNNATC 1 cut(s) 486
BseLI CCNNNNNNNGG 4 cut(s) 779, 911, 1208, 1349
BseMI GCAATG 2 cut(s) 113, 1196
BseMII CTCAG 2 cut(s) 260, 1176
BseNI ACTGG 1 cut(s) 531
BseXI GCAGC 3 cut(s) 159, 174, 658
BsgI GTGCAG 1 cut(s) 1305
BsiHKAI GWGCWC 2 cut(s) 594, 736
BsiSI CCGG 1 cut(s) 923
BslFI GGGAC 3 cut(s) 261, 1295, 1307
BslI CCNNNNNNNGG 4 cut(s) 779, 911, 1208, 1349
BsmAI GTCTC 2 cut(s) 1010, 1026
BsmBI CGTCTC 1 cut(s) 1010
BsmFI GGGAC 3 cut(s) 261, 1295, 1307
Bsp1286I GDGCHC 2 cut(s) 594, 736
Bsp1407I TGTACA 1 cut(s) 893
Bsp143I GATC 3 cut(s) 973, 1151, 1299
Bsp1720I GCTNAGC 1 cut(s) 1097
Bsp19I CCATGG 2 cut(s) 57, 1344
BspCNI CTCAG 2 cut(s) 261, 1177
BspHI TCATGA 1 cut(s) 16
BspLI GGNNCC 2 cut(s) 222, 608
BspMI ACCTGC 1 cut(s) 986
BspTI CTTAAG 2 cut(s) 253, 401
BsrDI GCAATG 2 cut(s) 113, 1196
BsrGI TGTACA 1 cut(s) 893
BsrI ACTGG 1 cut(s) 531
BssECI CCNNGG 3 cut(s) 57, 1202, 1344
BssMI GATC 3 cut(s) 973, 1151, 1299
BssNI GRCGYC 1 cut(s) 1354
BssSI CACGAG 1 cut(s) 683
BssT1I CCWWGG 3 cut(s) 57, 1202, 1344
Bst2BI CACGAG 1 cut(s) 683
Bst2UI CCWGG 1 cut(s) 218
Bst4CI ACNGT 1 cut(s) 742
BstACI GRCGYC 1 cut(s) 1354
BstAFI CTTAAG 2 cut(s) 253, 401
BstAUI TGTACA 1 cut(s) 893
BstC8I GCNNGC 4 cut(s) 549, 857, 957, 1079
BstDEI CTNAG 6 cut(s) 269, 338, 1088, 1097, 1148, 1185
BstDSI CCRYGG 2 cut(s) 57, 1344
BstF5I GGATG 2 cut(s) 78, 203
BstH2I RGCGCY 1 cut(s) 929
BstHHI GCGC 1 cut(s) 928
BstKTI GATC 3 cut(s) 976, 1154, 1302
BstMAI GTCTC 2 cut(s) 1010, 1026
BstMBI GATC 3 cut(s) 973, 1151, 1299
BstNI CCWGG 1 cut(s) 218
BstNSI RCATGY 5 cut(s) 101, 203, 1039, 1081, 1233
BstPAI GACNNNNGTC 1 cut(s) 1110
BstSCI CCNGG 1 cut(s) 216
BstSFI CTRYAG 2 cut(s) 372, 657
BstV1I GCAGC 3 cut(s) 159, 174, 658
BstX2I RGATCY 1 cut(s) 973
BstYI RGATCY 1 cut(s) 973
BtgI CCRYGG 2 cut(s) 57, 1344
BtgZI GCGATG 1 cut(s) 1282
BtsCI GGATG 2 cut(s) 78, 203
BveI ACCTGC 1 cut(s) 986
Cac8I GCNNGC 4 cut(s) 549, 857, 957, 1079
CciI TCATGA 1 cut(s) 16
CfoI GCGC 1 cut(s) 928
CseI GACGC 2 cut(s) 258, 1027
CsiI ACCWGGT 1 cut(s) 216
Csp6I GTAC 1 cut(s) 894
CviQI GTAC 1 cut(s) 894
DdeI CTNAG 6 cut(s) 269, 338, 1088, 1097, 1148, 1185
DpnI GATC 3 cut(s) 975, 1153, 1301
DpnII GATC 3 cut(s) 973, 1151, 1299
DraI TTTAAA 1 cut(s) 1260
Ecl136II GAGCTC 1 cut(s) 592
Eco130I CCWWGG 3 cut(s) 57, 1202, 1344
Eco24I GRGCYC 1 cut(s) 594
Eco32I GATATC 2 cut(s) 488, 578
Eco53kI GAGCTC 1 cut(s) 592
EcoICRI GAGCTC 1 cut(s) 592
EcoRII CCWGG 1 cut(s) 216
EcoRV GATATC 2 cut(s) 488, 578
EcoT14I CCWWGG 3 cut(s) 57, 1202, 1344
EcoT22I ATGCAT 2 cut(s) 6, 205
EcoT38I GRGCYC 1 cut(s) 594
ErhI CCWWGG 3 cut(s) 57, 1202, 1344
Esp3I CGTCTC 1 cut(s) 1010
FalI AAGNNNNNCTT 2 cut(s) 741, 773
FaqI GGGAC 3 cut(s) 261, 1295, 1307
FauNDI CATATG 1 cut(s) 226
Fnu4HI GCNGC 3 cut(s) 173, 188, 672
FokI GGATG 2 cut(s) 65, 190
FriOI GRGCYC 1 cut(s) 594
Fsp4HI GCNGC 3 cut(s) 173, 188, 672
FspBI CTAG 2 cut(s) 93, 698
GlaI GCGC 1 cut(s) 927
GluI GCNGC 3 cut(s) 173, 188, 672
HaeII RGCGCY 1 cut(s) 929
HapII CCGG 1 cut(s) 923
HgaI GACGC 2 cut(s) 258, 1027
HhaI GCGC 1 cut(s) 928
Hin1I GRCGYC 1 cut(s) 1354
Hin6I GCGC 1 cut(s) 926
HinP1I GCGC 1 cut(s) 926
HincII GTYRAC 1 cut(s) 917
HindII GTYRAC 1 cut(s) 917
HindIII AAGCTT 1 cut(s) 857
HinfI GANTC 8 cut(s) 265, 271, 299, 618, 842, 867, 1106, 1339
HpaII CCGG 1 cut(s) 923
HphI GGTGA 2 cut(s) 677, 1076
Hpy166II GTNNAC 2 cut(s) 896, 917
Hpy188I TCNGA 6 cut(s) 196, 270, 289, 1236, 1246, 1359
Hpy8I GTNNAC 2 cut(s) 896, 917
HpyAV CCTTC 6 cut(s) 332, 620, 915, 1143, 1202, 1343
HpyCH4III ACNGT 1 cut(s) 742
HpyCH4IV ACGT 1 cut(s) 1354
HpyCH4V TGCA 7 cut(s) 4, 203, 551, 627, 855, 1279, 1286
HpyF3I CTNAG 6 cut(s) 269, 338, 1088, 1097, 1148, 1185
HpySE526I ACGT 1 cut(s) 1354
Hsp92I GRCGYC 1 cut(s) 1354
HspAI GCGC 1 cut(s) 926
Kzo9I GATC 3 cut(s) 973, 1151, 1299
LmnI GCTCC 2 cut(s) 589, 1264
Lsp1109I GCAGC 3 cut(s) 159, 174, 658
LweI GCATC 4 cut(s) 212, 291, 636, 1259
MabI ACCWGGT 1 cut(s) 216
MaeI CTAG 2 cut(s) 93, 698
MaeII ACGT 1 cut(s) 1354
MaeIII GTNAC 5 cut(s) 326, 678, 792, 799, 1123
MalI GATC 3 cut(s) 975, 1153, 1301
MboI GATC 3 cut(s) 973, 1151, 1299
MboII GAAGA 7 cut(s) 102, 419, 460, 893, 929, 968, 1060
MfeI CAATTG 1 cut(s) 431
MflI RGATCY 1 cut(s) 973
MhlI GDGCHC 2 cut(s) 594, 736
MluCI AATT 8 cut(s) 310, 412, 431, 582, 829, 1118, 1239, 1360
MlyI GAGTC 4 cut(s) 280, 293, 836, 1100
MmeI TCCRAC 2 cut(s) 372, 687
Mph1103I ATGCAT 2 cut(s) 6, 205
MroXI GAANNNNTTC 1 cut(s) 1338
MseI TTAA 9 cut(s) 132, 254, 306, 402, 459, 494, 768, 818, 1259
MslI CAYNNNNRTG 2 cut(s) 162, 1228
MspCI CTTAAG 2 cut(s) 253, 401
MspI CCGG 1 cut(s) 923
MspR9I CCNGG 1 cut(s) 218
MunI CAATTG 1 cut(s) 431
MvaI CCWGG 1 cut(s) 218
NcoI CCATGG 2 cut(s) 57, 1344
NdeI CATATG 1 cut(s) 226
NdeII GATC 3 cut(s) 973, 1151, 1299
NlaIV GGNNCC 2 cut(s) 222, 608
NmuCI GTSAC 1 cut(s) 1123
NsiI ATGCAT 2 cut(s) 6, 205
NspI RCATGY 5 cut(s) 101, 203, 1039, 1081, 1233
PaeI GCATGC 1 cut(s) 1081
PagI TCATGA 1 cut(s) 16
PciI ACATGT 1 cut(s) 1229
PdmI GAANNNNTTC 1 cut(s) 1338
PfeI GAWTC 4 cut(s) 265, 618, 867, 1339
PkrI GCNGC 3 cut(s) 174, 189, 673
PleI GAGTC 4 cut(s) 279, 293, 836, 1100
PpsI GAGTC 4 cut(s) 279, 293, 836, 1100
PscI ACATGT 1 cut(s) 1229
PshAI GACNNNNGTC 1 cut(s) 1110
PsiI TTATAA 1 cut(s) 239
Psp124BI GAGCTC 1 cut(s) 594
Psp6I CCWGG 1 cut(s) 216
PspGI CCWGG 1 cut(s) 216
PspN4I GGNNCC 2 cut(s) 222, 608
PsuI RGATCY 1 cut(s) 973
RsaI GTAC 1 cut(s) 895
RsaNI GTAC 1 cut(s) 894
RseI CAYNNNNRTG 2 cut(s) 162, 1228
SacI GAGCTC 1 cut(s) 594
SaqAI TTAA 9 cut(s) 132, 254, 306, 402, 459, 494, 768, 818, 1259
SatI GCNGC 3 cut(s) 173, 188, 672
Sau3AI GATC 3 cut(s) 973, 1151, 1299
SchI GAGTC 4 cut(s) 280, 293, 836, 1100
ScrFI CCNGG 1 cut(s) 218
SduI GDGCHC 2 cut(s) 594, 736
SexAI ACCWGGT 1 cut(s) 216
SfaNI GCATC 4 cut(s) 212, 291, 636, 1259
SfcI CTRYAG 2 cut(s) 372, 657
SmiMI CAYNNNNRTG 2 cut(s) 162, 1228
SmlI CTYRAG 6 cut(s) 253, 401, 407, 593, 638, 860
SmoI CTYRAG 6 cut(s) 253, 401, 407, 593, 638, 860
SphI GCATGC 1 cut(s) 1081
Sse9I AATT 8 cut(s) 310, 412, 431, 582, 829, 1118, 1239, 1360
SspI AATATT 1 cut(s) 822
SspMI CTAG 2 cut(s) 93, 698
SstI GAGCTC 1 cut(s) 594
StyD4I CCNGG 1 cut(s) 216
StyI CCWWGG 3 cut(s) 57, 1202, 1344
TaaI ACNGT 1 cut(s) 742
TaiI ACGT 1 cut(s) 1357
TaqI TCGA 2 cut(s) 870, 1030
TasI AATT 8 cut(s) 310, 412, 431, 582, 829, 1118, 1239, 1360
TatI WGTACW 1 cut(s) 893
TfiI GAWTC 4 cut(s) 265, 618, 867, 1339
Tru1I TTAA 9 cut(s) 132, 254, 306, 402, 459, 494, 768, 818, 1259
Tru9I TTAA 9 cut(s) 132, 254, 306, 402, 459, 494, 768, 818, 1259
TseFI GTSAC 1 cut(s) 1123
TseI GCWGC 3 cut(s) 172, 187, 671
Tsp45I GTSAC 1 cut(s) 1123
TspDTI ATGAA 6 cut(s) 5, 102, 610, 738, 1083, 1347
TspGWI ACGGA 1 cut(s) 996
Vha464I CTTAAG 2 cut(s) 253, 401
XapI RAATTY 1 cut(s) 310
XceI RCATGY 5 cut(s) 101, 203, 1039, 1081, 1233
XmnI GAANNNNTTC 1 cut(s) 1338
XspI CTAG 2 cut(s) 93, 698
ZraI GACGTC 1 cut(s) 1355
Zsp2I ATGCAT 2 cut(s) 6, 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.