Rw7G022720

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001339
Physical Location & Seq
Reverse (-)
27027988 .. 27032422
4435 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G022720.1

Sequence Viewer

Length: 2271 bp
ATGCTGGTTCTCGAAATACTGTCTGTAGTATTGGGGACTGGCATCACATCGGCATTTCTGCCTGACGTTGCTAAGAAACTTGTTCAAGTCGGTCGAAAGTTGAGAGCACGAGATCCAGAGATCAATCAAAAGCTCCAAGAGTTGGAAAGAAGATTGTTGAAAATTCAATCCCATGTCGACGATGCCGAAAACAAGGCTTCTATCTCCAATGCCTACCAGATCATAATCGAAGATTTCAAGAATGTTCTTCTGGATGCAGATGATTTGCTGGACAAGACTCTTTTGGAGTATGAGAGGCTGTATTCTCAGAAAGTGAGGCCCGCCAAATTGGAAAAGGATTTGAAATCTTTTGAGGGAAAGCTCGGCAAAGAGATCGGTGAGATGGTGGCTAGGGTGAATGGTTTAGCAAGCGAAATGGAGGGTTACAGTATCTCAGAACTAAGCCAACCGAGGCCTGCTCGAACTTTTGCTCCTCCTCCTCAGATTATCAGAGGGTCGTTGGTTGATGAGCTCTCTGTAATTGGAAGGGAAAAGGATAAGCAGCAAATCCTGGAGTTGGTGTCTAAGCAGAGCAAGACAGCTGTTTCTGTGATTTCAATAGTGGGTATGGTTGGGATCGGGAAGACTACACTTGCCCAGCTAGTCTTCAATCATCAACATGGCAGTGACAAATTTAAGGCCGAAAGAAGGTGGTGGGTGAGTGTTTCTTTCGATGATGATGTGCAGAAGATCACCAAATCCATCCTTGAGTCCACTACGAAGACAGTTCCGGATTCCAGTATTGCGCAGTTGTCAAGTGTGGACTCTGTTCAGCTCAAATTAAAGGAGATTGTGGGGGACAAGGAGAGGTTTTTGCTCGTGCTAGATGGCATGTGTGATGAGAATCCTATCAATTGGGATGCCTTGATATTGCCATTTAGATCTGCAGCACCCGGGAGCATAATTGTGGTAACTACTCGAAGCCAAGTTGTTTCATCCATCGTTGCTGATCATGGCAGTACATATTGCTTGGGTGTTTTGTCAGATAAACATTGTTTGGAAATTATGAATAAAGGCTTGAGATCAAGGCTCAGTGAGAAGAGTGGCCTACAGATAGCAAAAAAGTGTAAAGGCCTTCCATTGGTGGCCAAATTGATTGGAAGTGTGATGCGTCTCAAATCAAAAGAGATGGAGTGGGATGAGATTCTAAAATGTGAGCTCTGGAACTTGGCAGAAGATAAGAATCTGATCTATCCAGTGCTTAAACTTGGGTATGACCATTTGCCAGCTTATTTGAAAAGATGTTTTGCTTATTCTTCTCTTCTCCCTCGTAATCTTCCCTTCCAAAGGGATGATTTGATCCAACTGTGGGCAGCTGAAGGCTTTATTGAAGCTCGAGGAACAAGAAGAATTGAAGATATTGGAAGAGAGTACTTTGATGACTTGTGCTCAAGGTCCTTTTTTCAAGTTCCATCAAAGAATCCTGGCGAAGGAAAGTATGAAATGCATGGCTTCATTCATGACTTGGCGAAAATAGTCTCAACCAACTTATGCTTTGCGTCTGAGGATACCAAGCCACACGTCTTGCCCATGTCTACAAATGTCCGGCATTCCTCAATTTGTGATGAAAAAGTTGAATCAGGTATGTTGGAAGAGTTTTACAAGTATAAGAAGTTAAGGACTTTTATGTTGCTCTCCAAGTACCCATACATGCTTCAAAGTGTTCCTGATGATTTCTTTCGGAAGTTTCCATGCTTAAGAGTGTTAAATTTGAGTGGTACTCGCATCTCTAAATTATCCGGAGATATTAAAATGTTGATACACCTTCGTCACCTCAATGTTAGCGGTACCTTGATCATAAAATTCCCACCGGGTATAGAGCAATTAAATTGGTTAGAAAGACTCAATGTCAAAAATTGTTCCAAACTTGAGGAGCTGCCTGAGAACTTAAACAAGTTGGTTAAGCTACGACATATTGACCTTGACAAACTCGGTAAAGTCACTTCCATGCCTGTTGATGTTGGGAAGTTAACTAGTCTTGAAACCCTGACTGTTTTTGTAGTGGGAAGTGAAGAAGGAAAAAAAATTGAGGAGCTCAAGGACATGAGGTGCCTCCGTGGATCCATTTGCATTCAAAAGCTTGAGAAAGTGAAAGATGACAGAGAAGCTAAGGCTGCTAAGTTACAAGAGAAGCAATACTTGGAGGAATTGGAGTTGCAATGGGAACCAAGGAGATCTGGAACAGTTGACCAAGCAGTTCTTGAGGGTCTTGAACCTCATCAGGCTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

756

Amino Acids

85.54

Weight (kDa)

8.31

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 36 - 100 3.2e-07 Rx N-terminal domain
NB-ARC PF00931 177 - 351 2.4e-25 NB-ARC domain
WHD_DRP PF23559 436 - 503 4.4e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 573 - 734 3e-20 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 689 - 754 4.8e-16 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 786
Acc65I GGTACC 1 cut(s) 1826
AccB1I GGYRCC 2 cut(s) 1826, 2088
AccB7I CCANNNNNTGG 1 cut(s) 142
AccI GTMKAC 2 cut(s) 177, 1574
AccIII TCCGGA 2 cut(s) 769, 1778
AciI CCGC 2 cut(s) 321, 1824
AclWI GGATC 5 cut(s) 107, 623, 1333, 2094, 2107
AcoI YGGCCR 1 cut(s) 1125
AcsI RAATTY 4 cut(s) 162, 671, 1747, 1841
AcuI CTGAAG 1 cut(s) 1377
AfaI GTAC 5 cut(s) 1000, 1412, 1682, 1759, 1828
AfiI CCNNNNNNNGG 7 cut(s) 142, 556, 687, 1120, 1326, 1348, 1469
AflII CTTAAG 1 cut(s) 1735
AflIII ACRYGT 1 cut(s) 1558
AhdI GACNNNNNGTC 1 cut(s) 1886
AhlI ACTAGT 1 cut(s) 2012
AjiI CACGTC 1 cut(s) 1561
AjnI CCWGG 2 cut(s) 549, 1462
AjuI GAANNNNNNNTTGG 2 cut(s) 2162, 2194
AloI GAACNNNNNNTCC 2 cut(s) 454, 486
Alw21I GWGCWC 5 cut(s) 109, 513, 1200, 1430, 2076
Alw26I GTCTC 2 cut(s) 1157, 1522
AlwI GGATC 5 cut(s) 107, 623, 1333, 2094, 2107
Ama87I CYCGRG 2 cut(s) 932, 1374
Aor13HI TCCGGA 2 cut(s) 769, 1778
AoxI GGCC 6 cut(s) 317, 452, 678, 1084, 1111, 1125
ApeKI GCWGC 5 cut(s) 541, 926, 1352, 1915, 2153
ApoI RAATTY 4 cut(s) 162, 671, 1747, 1841
Asp718I GGTACC 1 cut(s) 1826
AspLEI GCGC 1 cut(s) 787
AspS9I GGNCC 2 cut(s) 318, 1434
AsuC2I CCSGG 3 cut(s) 933, 934, 1851
AsuHPI GGTGA 5 cut(s) 389, 406, 709, 724, 1802
AvaI CYCGRG 2 cut(s) 932, 1374
AvaII GGWCC 1 cut(s) 1434
BalI TGGCCA 1 cut(s) 1127
BamHI GGATCC 1 cut(s) 2099
BanI GGYRCC 2 cut(s) 1826, 2088
BanII GRGCYC 3 cut(s) 513, 1200, 2076
BarI GAAGNNNNNNTAC 2 cut(s) 1966, 1998
BauI CACGAG 2 cut(s) 108, 857
BbsI GAAGAC 3 cut(s) 629, 637, 767
Bbv12I GWGCWC 5 cut(s) 109, 513, 1200, 1430, 2076
BbvI GCAGC 5 cut(s) 553, 938, 1364, 1902, 2140
BccI CCATC 6 cut(s) 376, 749, 860, 986, 1162, 1459
BcgI CGANNNNNNTGC 2 cut(s) 355, 389
BciT130I CCWGG 2 cut(s) 551, 1464
BciVI GTATCC 1 cut(s) 1540
BclI TGATCA 2 cut(s) 988, 1833
BcnI CCSGG 3 cut(s) 933, 934, 1851
BcoDI GTCTC 2 cut(s) 1157, 1522
BcuI ACTAGT 1 cut(s) 2012
BfaI CTAG 4 cut(s) 390, 641, 863, 2013
BfmI CTRYAG 3 cut(s) 24, 924, 1088
BfrI CTTAAG 1 cut(s) 1735
BfuI GTATCC 1 cut(s) 1540
BglII AGATCT 2 cut(s) 920, 2213
BisI GCNGC 5 cut(s) 542, 927, 1353, 1916, 2154
BlsI GCNGC 5 cut(s) 543, 928, 1354, 1917, 2155
BmcAI AGTACT 1 cut(s) 1412
Bme1390I CCNGG 5 cut(s) 551, 933, 934, 1464, 1851
Bme18I GGWCC 1 cut(s) 1434
BmeRI GACNNNNNGTC 1 cut(s) 1886
BmeT110I CYCGRG 2 cut(s) 932, 1374
BmgBI CACGTC 1 cut(s) 1561
BmgT120I GGNCC 2 cut(s) 318, 1434
BmiI GGNNCC 4 cut(s) 1828, 2090, 2101, 2205
BmrFI CCNGG 5 cut(s) 551, 933, 934, 1464, 1851
BmsI GCATC 6 cut(s) 51, 172, 244, 889, 1137, 1773
BpiI GAAGAC 3 cut(s) 629, 637, 767
BplI GAGNNNNNCTC 4 cut(s) 442, 474, 1744, 1776
BpmI CTGGAG 1 cut(s) 572
Bpu10I CCTNAGC 1 cut(s) 2148
BpuEI CTTGAG 7 cut(s) 767, 1078, 1414, 1928, 2060, 2141, 2261
BpuMI CCSGG 3 cut(s) 933, 934, 1851
BsaBI GATNNNNATC 3 cut(s) 224, 882, 1221
BsaJI CCNNGG 4 cut(s) 449, 932, 2095, 2207
BsaWI WCCGGW 2 cut(s) 769, 1778
BsaXI ACNNNNNCTCC 4 cut(s) 454, 484, 545, 575
Bsc4I CCNNNNNNNGG 7 cut(s) 142, 556, 687, 1120, 1326, 1348, 1469
Bse1I ACTGG 3 cut(s) 43, 777, 1235
Bse3DI GCAATG 1 cut(s) 2204
Bse8I GATNNNNATC 3 cut(s) 224, 882, 1221
BseAI TCCGGA 2 cut(s) 769, 1778
BseBI CCWGG 2 cut(s) 551, 1464
BseDI CCNNGG 4 cut(s) 449, 932, 2095, 2207
BseGI GGATG 6 cut(s) 259, 741, 904, 974, 1183, 1336
BseJI GATNNNNATC 3 cut(s) 224, 882, 1221
BseLI CCNNNNNNNGG 7 cut(s) 142, 556, 687, 1120, 1326, 1348, 1469
BseMI GCAATG 1 cut(s) 2204
BseMII CTCAG 6 cut(s) 320, 447, 494, 1084, 1533, 1911
BseNI ACTGG 3 cut(s) 43, 777, 1235
BseRI GAGGAG 5 cut(s) 462, 465, 468, 1925, 2084
BseXI GCAGC 5 cut(s) 553, 938, 1364, 1902, 2140
BseYI CCCAGC 1 cut(s) 636
BsgI GTGCAG 1 cut(s) 743
Bsh1285I CGRYCG 1 cut(s) 94
BshFI GGCC 6 cut(s) 319, 454, 680, 1086, 1113, 1127
BshNI GGYRCC 2 cut(s) 1826, 2088
BsiEI CGRYCG 1 cut(s) 94
BsiHKAI GWGCWC 5 cut(s) 109, 513, 1200, 1430, 2076
BsiHKCI CYCGRG 2 cut(s) 932, 1374
BsiSI CCGG 5 cut(s) 770, 933, 1585, 1779, 1850
BslFI GGGAC 2 cut(s) 49, 851
BslI CCNNNNNNNGG 7 cut(s) 142, 556, 687, 1120, 1326, 1348, 1469
BsmAI GTCTC 2 cut(s) 1157, 1522
BsmBI CGTCTC 1 cut(s) 1157
BsmFI GGGAC 2 cut(s) 49, 851
BsmI GAATGC 2 cut(s) 1588, 2109
BsnI GGCC 6 cut(s) 319, 454, 680, 1086, 1113, 1127
BsoBI CYCGRG 2 cut(s) 932, 1374
Bsp1286I GDGCHC 5 cut(s) 109, 513, 1200, 1430, 2076
Bsp13I TCCGGA 2 cut(s) 769, 1778
BspACI CCGC 2 cut(s) 321, 1824
BspANI GGCC 6 cut(s) 319, 454, 680, 1086, 1113, 1127
BspCNI CTCAG 6 cut(s) 319, 446, 493, 1083, 1534, 1912
BspEI TCCGGA 2 cut(s) 769, 1778
BspHI TCATGA 1 cut(s) 1498
BspLI GGNNCC 4 cut(s) 1828, 2090, 2101, 2205
BspMAI CTGCAG 1 cut(s) 928
BspPI GGATC 5 cut(s) 107, 623, 1333, 2094, 2107
BspT107I GGYRCC 2 cut(s) 1826, 2088
BspTI CTTAAG 1 cut(s) 1735
BsrDI GCAATG 1 cut(s) 2204
BsrI ACTGG 3 cut(s) 43, 777, 1235
BssECI CCNNGG 4 cut(s) 449, 932, 2095, 2207
BssSI CACGAG 2 cut(s) 108, 857
BssT1I CCWWGG 1 cut(s) 2207
Bst2BI CACGAG 2 cut(s) 108, 857
Bst2UI CCWGG 2 cut(s) 551, 1464
Bst4CI ACNGT 6 cut(s) 21, 428, 766, 1347, 2032, 2224
Bst6I CTCTTC 4 cut(s) 1073, 1305, 1399, 1626
BstAFI CTTAAG 1 cut(s) 1735
BstC8I GCNNGC 4 cut(s) 321, 409, 456, 1266
BstDSI CCRYGG 1 cut(s) 2095
BstENI CCTNNNNNAGG 2 cut(s) 1324, 1467
BstF5I GGATG 6 cut(s) 259, 741, 904, 974, 1183, 1336
BstHHI GCGC 1 cut(s) 787
BstMAI GTCTC 2 cut(s) 1157, 1522
BstMCI CGRYCG 1 cut(s) 94
BstMWI GCNNNNNNNGC 1 cut(s) 2153
BstNI CCWGG 2 cut(s) 551, 1464
BstNSI RCATGY 2 cut(s) 874, 1693
BstSCI CCNGG 5 cut(s) 549, 931, 932, 1462, 1849
BstSFI CTRYAG 3 cut(s) 24, 924, 1088
BstV1I GCAGC 5 cut(s) 553, 938, 1364, 1902, 2140
BstV2I GAAGAC 3 cut(s) 629, 637, 767
BstX2I RGATCY 4 cut(s) 112, 920, 2099, 2213
BstYI RGATCY 4 cut(s) 112, 920, 2099, 2213
BsuI GTATCC 1 cut(s) 1540
BsuRI GGCC 6 cut(s) 319, 454, 680, 1086, 1113, 1127
BtgI CCRYGG 1 cut(s) 2095
BtrI CACGTC 1 cut(s) 1561
BtsCI GGATG 6 cut(s) 259, 741, 904, 974, 1183, 1336
BtsI GCAGTG 1 cut(s) 670
BtsIMutI CAGTG 3 cut(s) 670, 1078, 1242
Cac8I GCNNGC 4 cut(s) 321, 409, 456, 1266
CciI TCATGA 1 cut(s) 1498
CfoI GCGC 1 cut(s) 787
Cfr13I GGNCC 2 cut(s) 318, 1434
Cfr9I CCCGGG 1 cut(s) 932
CseI GACGC 2 cut(s) 1139, 1527
Csp6I GTAC 5 cut(s) 999, 1411, 1681, 1758, 1827
CspCI CAANNNNNGTGG 2 cut(s) 1545, 1580
CviQI GTAC 5 cut(s) 999, 1411, 1681, 1758, 1827
DriI GACNNNNNGTC 1 cut(s) 1886
EaeI YGGCCR 1 cut(s) 1125
Eam1104I CTCTTC 4 cut(s) 1073, 1305, 1399, 1626
Eam1105I GACNNNNNGTC 1 cut(s) 1886
EarI CTCTTC 4 cut(s) 1073, 1305, 1399, 1626
Ecl136II GAGCTC 3 cut(s) 511, 1198, 2074
Eco130I CCWWGG 1 cut(s) 2207
Eco147I AGGCCT 2 cut(s) 454, 1113
Eco24I GRGCYC 3 cut(s) 513, 1200, 2076
Eco47I GGWCC 1 cut(s) 1434
Eco53kI GAGCTC 3 cut(s) 511, 1198, 2074
Eco57I CTGAAG 1 cut(s) 1377
Eco88I CYCGRG 2 cut(s) 932, 1374
EcoICRI GAGCTC 3 cut(s) 511, 1198, 2074
EcoNI CCTNNNNNAGG 2 cut(s) 1324, 1467
EcoO109I RGGNCCY 1 cut(s) 1434
EcoRII CCWGG 2 cut(s) 549, 1462
EcoT14I CCWWGG 1 cut(s) 2207
EcoT22I ATGCAT 1 cut(s) 1488
EcoT38I GRGCYC 3 cut(s) 513, 1200, 2076
ErhI CCWWGG 1 cut(s) 2207
Esp3I CGTCTC 1 cut(s) 1157
FalI AAGNNNNNCTT 4 cut(s) 2162, 2194, 2223, 2255
FaqI GGGAC 2 cut(s) 49, 851
FauI CCCGC 1 cut(s) 328
FbaI TGATCA 2 cut(s) 988, 1833
FblI GTMKAC 2 cut(s) 177, 1574
Fnu4HI GCNGC 5 cut(s) 542, 927, 1353, 1916, 2154
FokI GGATG 6 cut(s) 266, 728, 911, 961, 1190, 1343
FriOI GRGCYC 3 cut(s) 513, 1200, 2076
Fsp4HI GCNGC 5 cut(s) 542, 927, 1353, 1916, 2154
FspBI CTAG 4 cut(s) 390, 641, 863, 2013
FspI TGCGCA 1 cut(s) 786
GlaI GCGC 1 cut(s) 786
GluI GCNGC 5 cut(s) 542, 927, 1353, 1916, 2154
GsaI CCCAGC 1 cut(s) 640
GsuI CTGGAG 1 cut(s) 572
HaeIII GGCC 6 cut(s) 319, 454, 680, 1086, 1113, 1127
HapII CCGG 5 cut(s) 770, 933, 1585, 1779, 1850
HgaI GACGC 2 cut(s) 1139, 1527
HhaI GCGC 1 cut(s) 787
Hin6I GCGC 1 cut(s) 785
HinP1I GCGC 1 cut(s) 785
HincII GTYRAC 3 cut(s) 178, 2010, 2227
HindII GTYRAC 3 cut(s) 178, 2010, 2227
HindIII AAGCTT 1 cut(s) 2117
HpaI GTTAAC 1 cut(s) 2010
HpaII CCGG 5 cut(s) 770, 933, 1585, 1779, 1850
HphI GGTGA 5 cut(s) 389, 406, 709, 724, 1802
Hpy166II GTNNAC 6 cut(s) 178, 753, 802, 1575, 2010, 2227
Hpy188I TCNGA 8 cut(s) 309, 436, 483, 491, 1024, 1227, 1543, 1722
Hpy8I GTNNAC 6 cut(s) 178, 753, 802, 1575, 2010, 2227
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 8 cut(s) 519, 681, 1124, 1330, 1352, 1463, 1814, 2048
HpyCH4III ACNGT 6 cut(s) 21, 428, 766, 1347, 2032, 2224
HpyCH4IV ACGT 2 cut(s) 66, 1560
HpyCH4V TGCA 6 cut(s) 257, 724, 926, 1486, 2109, 2197
HpyF10VI GCNNNNNNNGC 1 cut(s) 2153
HpySE526I ACGT 2 cut(s) 66, 1560
HspAI GCGC 1 cut(s) 785
Kpn2I TCCGGA 2 cut(s) 769, 1778
KpnI GGTACC 1 cut(s) 1830
Ksp22I TGATCA 2 cut(s) 988, 1833
KspAI GTTAAC 1 cut(s) 2010
LmnI GCTCC 5 cut(s) 138, 475, 936, 1912, 2071
Lsp1109I GCAGC 5 cut(s) 553, 938, 1364, 1902, 2140
LweI GCATC 6 cut(s) 51, 172, 244, 889, 1137, 1773
MaeI CTAG 4 cut(s) 390, 641, 863, 2013
MaeII ACGT 2 cut(s) 66, 1560
MaeIII GTNAC 6 cut(s) 422, 665, 949, 1808, 1978, 2160
MfeI CAATTG 1 cut(s) 892
MflI RGATCY 4 cut(s) 112, 920, 2099, 2213
MhlI GDGCHC 5 cut(s) 109, 513, 1200, 1430, 2076
MlsI TGGCCA 1 cut(s) 1127
MluNI TGGCCA 1 cut(s) 1127
MlyI GAGTC 4 cut(s) 271, 758, 797, 1875
MmeI TCCRAC 3 cut(s) 123, 1366, 1608
Mox20I TGGCCA 1 cut(s) 1127
Mph1103I ATGCAT 1 cut(s) 1488
MroI TCCGGA 2 cut(s) 769, 1778
MscI TGGCCA 1 cut(s) 1127
MslI CAYNNNNRTG 5 cut(s) 657, 663, 944, 1815, 1985
Msp20I TGGCCA 1 cut(s) 1127
MspA1I CMGCKG 2 cut(s) 581, 1355
MspCI CTTAAG 1 cut(s) 1735
MspI CCGG 5 cut(s) 770, 933, 1585, 1779, 1850
MspR9I CCNGG 5 cut(s) 551, 933, 934, 1464, 1851
MunI CAATTG 1 cut(s) 892
Mva1269I GAATGC 2 cut(s) 1588, 2109
MvaI CCWGG 2 cut(s) 551, 1464
MwoI GCNNNNNNNGC 1 cut(s) 2153
NciI CCSGG 3 cut(s) 933, 934, 1851
NlaIV GGNNCC 4 cut(s) 1828, 2090, 2101, 2205
NmeAIII GCCGAG 1 cut(s) 342
NmuCI GTSAC 3 cut(s) 665, 1808, 1978
NsbI TGCGCA 1 cut(s) 786
NsiI ATGCAT 1 cut(s) 1488
NspI RCATGY 2 cut(s) 874, 1693
PaeR7I CTCGAG 1 cut(s) 1374
PagI TCATGA 1 cut(s) 1498
PceI AGGCCT 2 cut(s) 454, 1113
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 2 cut(s) 1588, 2109
PfeI GAWTC 6 cut(s) 773, 883, 1183, 1222, 1459, 1616
PflMI CCANNNNNTGG 1 cut(s) 142
PfoI TCCNGGA 1 cut(s) 549
PkrI GCNGC 5 cut(s) 543, 928, 1354, 1917, 2155
PleI GAGTC 4 cut(s) 271, 757, 797, 1875
PpsI GAGTC 4 cut(s) 271, 757, 797, 1875
PpuMI RGGWCCY 1 cut(s) 1434
Psp124BI GAGCTC 3 cut(s) 513, 1200, 2076
Psp5II RGGWCCY 1 cut(s) 1434
Psp6I CCWGG 2 cut(s) 549, 1462
PspFI CCCAGC 1 cut(s) 636
PspGI CCWGG 2 cut(s) 549, 1462
PspN4I GGNNCC 4 cut(s) 1828, 2090, 2101, 2205
PspPI GGNCC 2 cut(s) 318, 1434
PspPPI RGGWCCY 1 cut(s) 1434
PspXI VCTCGAGB 1 cut(s) 1374
PstI CTGCAG 1 cut(s) 928
PsuI RGATCY 4 cut(s) 112, 920, 2099, 2213
PvuII CAGCTG 2 cut(s) 581, 1355
RsaI GTAC 5 cut(s) 1000, 1412, 1682, 1759, 1828
RsaNI GTAC 5 cut(s) 999, 1411, 1681, 1758, 1827
RseI CAYNNNNRTG 5 cut(s) 657, 663, 944, 1815, 1985
SacI GAGCTC 3 cut(s) 513, 1200, 2076
SalI GTCGAC 1 cut(s) 176
SatI GCNGC 5 cut(s) 542, 927, 1353, 1916, 2154
Sau96I GGNCC 2 cut(s) 318, 1434
ScaI AGTACT 1 cut(s) 1412
SchI GAGTC 4 cut(s) 271, 758, 797, 1875
ScrFI CCNGG 5 cut(s) 551, 933, 934, 1464, 1851
SduI GDGCHC 5 cut(s) 109, 513, 1200, 1430, 2076
SfaNI GCATC 6 cut(s) 51, 172, 244, 889, 1137, 1773
SfcI CTRYAG 3 cut(s) 24, 924, 1088
Sfr274I CTCGAG 1 cut(s) 1374
SinI GGWCC 1 cut(s) 1434
SlaI CTCGAG 1 cut(s) 1374
SmaI CCCGGG 1 cut(s) 934
SmiMI CAYNNNNRTG 5 cut(s) 657, 663, 944, 1815, 1985
SmlI CTYRAG 9 cut(s) 746, 1057, 1374, 1429, 1735, 1907, 2075, 2120, 2240
SmoI CTYRAG 9 cut(s) 746, 1057, 1374, 1429, 1735, 1907, 2075, 2120, 2240
SpeI ACTAGT 1 cut(s) 2012
SseBI AGGCCT 2 cut(s) 454, 1113
SsiI CCGC 2 cut(s) 321, 1824
SspMI CTAG 4 cut(s) 390, 641, 863, 2013
SstI GAGCTC 3 cut(s) 513, 1200, 2076
StuI AGGCCT 2 cut(s) 454, 1113
StyD4I CCNGG 5 cut(s) 549, 931, 932, 1462, 1849
StyI CCWWGG 1 cut(s) 2207
TaaI ACNGT 6 cut(s) 21, 428, 766, 1347, 2032, 2224
TaiI ACGT 2 cut(s) 69, 1563
TaqI TCGA 8 cut(s) 12, 94, 177, 228, 460, 711, 958, 1375
TaqII GACCGA 1 cut(s) 80
TatI WGTACW 2 cut(s) 998, 1410
TfiI GAWTC 6 cut(s) 773, 883, 1183, 1222, 1459, 1616
TscAI CASTG 3 cut(s) 670, 1078, 1242
TseFI GTSAC 3 cut(s) 665, 1808, 1978
TseI GCWGC 5 cut(s) 541, 926, 1352, 1915, 2153
Tsp45I GTSAC 3 cut(s) 665, 1808, 1978
TspDTI ATGAA 6 cut(s) 963, 1061, 1483, 1487, 1494, 1620
TspGWI ACGGA 1 cut(s) 2084
TspMI CCCGGG 1 cut(s) 932
TspRI CASTG 3 cut(s) 670, 1078, 1242
Van91I CCANNNNNTGG 1 cut(s) 142
Vha464I CTTAAG 1 cut(s) 1735
VpaK11BI GGWCC 1 cut(s) 1434
XagI CCTNNNNNAGG 2 cut(s) 1324, 1467
XapI RAATTY 4 cut(s) 162, 671, 1747, 1841
XceI RCATGY 2 cut(s) 874, 1693
XhoI CTCGAG 1 cut(s) 1374
XmaI CCCGGG 1 cut(s) 932
XmiI GTMKAC 2 cut(s) 177, 1574
XspI CTAG 4 cut(s) 390, 641, 863, 2013
ZrmI AGTACT 1 cut(s) 1412
Zsp2I ATGCAT 1 cut(s) 1488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.