RchiOBHm_Chr4g0433191

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001339
Physical Location & Seq
Reverse (-)
57354306 .. 57359222
4917 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40171

Sequence Viewer

Length: 3012 bp
ATGGCAGAGATAGCATCCCTGGTACTCTCGACCGGCATTACGTCGGCAATTCTTCAGGTTGTTGTTGACAGAGTCGCAAAATTTGTTGAACAAAAGAGAGACATGTTCACTGGGGTCGACGACAATCTCAGAAAGTTGAAGAGAACGCTGATGAAGATTCAAGCTCGTGTTGATGATATAGAAAAGAAACATTGCATCACGGAGGCAGCAGAAGATTTACTGGAAGATCTGCTGACTAGTTTTTTGGATGCTGAAGATTTGCTGGACAAGATCGGTTTGGGACTGGAAAGGATGACTTGTTTGGATGTGGAGGTCAACAGTTGGAGTCAGGTACATCACTTGATCCCCTTCTATTCTGGCATACCGTCTGAGATTAAAAAGATGCAAGTGAAATTGGATGAGTTAGTAACAGAACTAGAAAGCTACCCTACCACGGAAGTCATCCGGTTGAGAAAGCCTATAGAAAAGGCTATTGCTCCTTGTACTTCTTTGGTTGATGAATCTTGTGTATTTGGAAGGGAAACAGATAAGAACAAGATGAAAAAAATGGTCTTGTCTCTGCAAGTAGAGGGGGGAAATGTTTCTGTAATTTCAGTAGTAGGGATGGCTGGGATTGGGAAAACAACACTTGCCCAACTCGTCTACAATGATGCAGATGTGCATAAGAAATTTGATTTGAAAATGTGGGTGTCTGTTTCTTTAGAGTGTGGTCTGTACATGATTACAAAATCTATTCTTGAGTCTGCTACTAGAGGAAAGGGTTCTCAGTTGTCAAGTTTGGATAGTGTTCAGATTGAATTACAGAAGACATTGAAGGGGAAGAGGTTTTTACTTGTACTAGATGGCATTTGTAATGATAATCTTAGTGATTGGGATCAATTCAGGTTGTCTTTTGGGGCTGCAAAGAAGGGGAGCAAGATTATGATGACTACTCGAAGTGAAATTTCTGCCTCAGTTGTTGCCTCTACATTATATCGTTTGGATCCTCTGTGTGACAATGCTTGTTGGGAAATAATCAAACAAAGAGGGAATCTTAATGGGCAGACTAATTTGGAGGCGAATGGCCTAGCAATTTCGAAGAAGTGCAAAGGGTTTCCATGGGTAGCAAAAGTGATTGGAAGCTGTTTGAATTCGGGAGAGATGGAGTGGGATGCTCTGTTAAGGTGTGAGCTTTGGGAACTGCAGGAACATAAAACACAAATCTATCCAGTCCTCAAATTGTTGAGCTATGATAATATGCCTTCTTATTTGAAAAGATGTTTCAGTTATTGTTCTATCTTTCCTCGTGATCATGTAGTTGAGAAGGATGATTTGGTCCAACTATGGGCAGCAGAAGGCTTCATTCAGTCCCAAGGAACAAGAAAAATTGAAGATGTTGGAAGACAGTATTTTGAGGACTTGTGTTCGAGGGCTTTCTTTTCGCAAGTTCCAAATATTGGTGGTGATCTGCAAAGATATAAAATGCATGAGCTCATTCATGATTTGGCAAGATTTGTTTCAACCAACTTATGCTTTTGTATGGATGACAATGTGCCATGCTTGGTGCACATATCTCCTAATGCTCGGCATGCGTCTTTGCTCTATGAAAATACTCAACTATATACATTGGAGGCTTTCTACGAGTATAAGAAGTTGAGGACATTTACATTGCTTCCTAAGTCCCCATCTATTCCGAGGCCAGTCATGTTGCTTCCCAATTTTTCGTCCAATCTGGGCGAAGTCCCTCATGAATTTTTTGAAAGGTTGGGAAGCTTGAGGGTGTTGAATTTGAGTCATAGTGCCATCTCTAAATTGCCTGAATCTATTGGAAATTTGATACATCTTCGTTACCTCAATCTTAGTCATACCCGTATTGAACTATTGCCAAAGCCTATAACAACAATATATGGGTTAGAAACATTGAAGCTCAAGAGTTGTCCTAAGCTTCTTCATTTGCCTGAGAACTTAAAGGACTTGATTAAGCTAAGACATCTTGACTTTGACAGACACCGTCAACTAAGTTCCATGCCAAAAGATGTTGGGAAGTTAACTAGTCTCGAAACCCTTCATGCATTTAAAGTGGGAAAAGAGAAAGGATATCAGATTGAGGAGCTCAAGAACATGAGGTGTCTTCGTGGATCCATTTCTATTACGAATCTTGAGAATGTGGCAGATTCTATGCAGGCTGAGGCTGCTATGTTACACAACAAGCAATACTTAGACAGGTTGGAGCTGGAATGGAATGCAATCAGAGGTCAAATGCAACCAGTTCAACAAGAAGTTCTTACAGGGCTTAAACCACATGCTGGGTTGAAGGAGTTACGAGTAGCTGGTTACTGTGGTTCGGTATTTCCCGGATGGATAGCCAACTCAGCATTTAGCAGGCTTGAGAGTATTGAATTAGAAAATTGCGCATTTTGTGAACTCCTTCCTTCTCTTGGCCAACTTCCGGCTCTCAAGAAACTTCTTATTCAAAATATGGCTACTTTGAAAGGTGTGGATTATCTGTTTTGTGGTTCCTCAGCTGCTTTTCTATCCTTAGAGACGCTAACACTTCGTGACATGCCGGAGTTGAGTAACTGGTCAGGACTACGTGACAATGACATGCCTTTCCTCAGAGACCTCAGCATTGATTCTTGTCCAAAGTTGGCCACCCTACCTTCATTTCATTACCTAACTTCACTTCAATGTTTAGACATCAATCGTTGCCCTCAGCTTCAATCTTTGCCAGAGGGAGGACTGCCATCCTCACTCAAAACTTTGGTCATTATGGAGAGTGGCATCTTGAAGGATCGGTGCAAGGTGGGAGAAGGTGCAGATTGGAATAAGATCAGATGGATCCCTGCCATATTGATTGAAAATATGGTGATTCCAGCACAAAGACCGGATAACCAGATTGATGAGGTTCAGAGTGCTCCAGAAAACATGTTTTGCAGCCGAAACTCCTTGTTGACTTGTTCTGTTATTCTTTTTCCTCTGCTCTTCCTTTTTATGAGATCATTGTACCAGTACTTGTCCCAAGCCAGATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1003

Amino Acids

113.32

Weight (kDa)

6.74

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 18 - 98 7.9e-10 Rx N-terminal domain
NB-ARC PF00931 180 - 343 4.5e-30 NB-ARC domain
WHD_DRP PF23559 426 - 496 5.5e-25 Disease resistance protein Winged helix domain
LRR_8 PF13855 568 - 618 5.4e-09 Leucine rich repeat
LRR_14 PF23598 582 - 872 4.4e-30 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 695 - 820 9.3e-38 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2392
AccB7I CCANNNNNTGG 2 cut(s) 1436, 2285
AccI GTMKAC 2 cut(s) 117, 642
AclWI GGATC 9 cut(s) 337, 882, 977, 990, 2112, 2125, 2778, 2812, 2825
AcoI YGGCCR 2 cut(s) 2419, 2628
AcsI RAATTY 7 cut(s) 80, 668, 942, 1129, 1730, 1765, 1810
AcuI CTGAAG 2 cut(s) 38, 273
AdeI CACNNNGTG 1 cut(s) 2537
AfaI GTAC 7 cut(s) 24, 333, 484, 716, 837, 2984, 2990
AfiI CCNNNNNNNGG 7 cut(s) 433, 1324, 1436, 2285, 2417, 2428, 2714
AflIII ACRYGT 2 cut(s) 102, 2904
AhlI ACTAGT 2 cut(s) 236, 2030
AjnI CCWGG 1 cut(s) 18
AjuI GAANNNNNNNTTGG 2 cut(s) 1295, 1327
Alw21I GWGCWC 4 cut(s) 1473, 1548, 2094, 2896
Alw26I GTCTC 5 cut(s) 93, 561, 2039, 2516, 2592
Alw44I GTGCAC 1 cut(s) 1544
AlwI GGATC 9 cut(s) 337, 882, 977, 990, 2112, 2125, 2778, 2812, 2825
AoxI GGCC 4 cut(s) 1063, 1676, 2419, 2628
ApaLI GTGCAC 1 cut(s) 1544
ApeKI GCWGC 6 cut(s) 206, 899, 1328, 2171, 2504, 2913
ApoI RAATTY 7 cut(s) 80, 668, 942, 1129, 1730, 1765, 1810
ArsI GACNNNNNNTTYG 4 cut(s) 226, 258, 2727, 2759
Asp700I GAANNNNTTC 4 cut(s) 580, 760, 2043, 2406
AspLEI GCGC 1 cut(s) 2393
AspS9I GGNCC 1 cut(s) 1315
AsuC2I CCSGG 1 cut(s) 2334
AsuHPI GGTGA 2 cut(s) 1454, 2857
AsuII TTCGAA 1 cut(s) 1076
AvaII GGWCC 1 cut(s) 1315
BaeGI GKGCMC 1 cut(s) 1548
BalI TGGCCA 2 cut(s) 2421, 2630
BamHI GGATCC 3 cut(s) 982, 2117, 2817
BanII GRGCYC 2 cut(s) 1473, 2094
BauI CACGAG 2 cut(s) 165, 1284
BbsI GAAGAC 3 cut(s) 812, 1387, 2102
Bbv12I GWGCWC 4 cut(s) 1473, 1548, 2094, 2896
BbvCI CCTCAGC 4 cut(s) 2166, 2500, 2603, 2691
BbvI GCAGC 6 cut(s) 218, 886, 1340, 2158, 2491, 2925
BccI CCATC 8 cut(s) 598, 836, 1135, 1672, 1790, 2331, 2731, 2808
BciT130I CCWGG 1 cut(s) 20
BclI TGATCA 1 cut(s) 1288
BcnI CCSGG 1 cut(s) 2334
BcoDI GTCTC 5 cut(s) 93, 561, 2039, 2516, 2592
BcuI ACTAGT 2 cut(s) 236, 2030
BfaI CTAG 6 cut(s) 237, 416, 750, 839, 1067, 2031
BfmI CTRYAG 2 cut(s) 459, 1181
BglII AGATCT 1 cut(s) 226
BisI GCNGC 6 cut(s) 207, 900, 1329, 2172, 2505, 2914
BlsI GCNGC 6 cut(s) 208, 901, 1330, 2173, 2506, 2915
BmcAI AGTACT 1 cut(s) 2990
Bme1390I CCNGG 2 cut(s) 20, 2334
Bme18I GGWCC 1 cut(s) 1315
BmgT120I GGNCC 1 cut(s) 1315
BmiI GGNNCC 4 cut(s) 984, 2119, 2497, 2819
BmrFI CCNGG 2 cut(s) 20, 2334
BmrI ACTGGG 1 cut(s) 120
BmsI GCATC 7 cut(s) 23, 204, 238, 372, 640, 1141, 2769
BmuI ACTGGG 1 cut(s) 120
BpiI GAAGAC 3 cut(s) 812, 1387, 2102
BpmI CTGGAG 1 cut(s) 2880
Bpu10I CCTNAGC 5 cut(s) 1920, 2166, 2500, 2603, 2691
Bpu14I TTCGAA 1 cut(s) 1076
BpuEI CTTGAG 7 cut(s) 758, 1774, 1892, 2078, 2159, 2387, 2420
BpuMI CCSGG 1 cut(s) 2334
BsaAI YACGTR 1 cut(s) 2573
BsaBI GATNNNNATC 1 cut(s) 873
BsaI GGTCTC 1 cut(s) 2592
BsaJI CCNNGG 5 cut(s) 18, 432, 1097, 1351, 1673
BsaWI WCCGGW 2 cut(s) 444, 2863
Bsc4I CCNNNNNNNGG 7 cut(s) 433, 1324, 1436, 2285, 2417, 2428, 2714
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 8 cut(s) 115, 225, 288, 1208, 1679, 2246, 2564, 2986
Bse3DI GCAATG 2 cut(s) 190, 1646
Bse8I GATNNNNATC 1 cut(s) 873
BseBI CCWGG 1 cut(s) 20
BseDI CCNNGG 5 cut(s) 18, 432, 1097, 1351, 1673
BseJI GATNNNNATC 1 cut(s) 873
BseLI CCNNNNNNNGG 7 cut(s) 433, 1324, 1436, 2285, 2417, 2428, 2714
BseMI GCAATG 2 cut(s) 190, 1646
BseNI ACTGG 8 cut(s) 115, 225, 288, 1208, 1679, 2246, 2564, 2986
BseRI GAGGAG 1 cut(s) 2102
BseSI GKGCMC 1 cut(s) 1548
BseXI GCAGC 6 cut(s) 218, 886, 1340, 2158, 2491, 2925
BseYI CCCAGC 2 cut(s) 608, 2285
BsgI GTGCAG 1 cut(s) 2814
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 4 cut(s) 1065, 1678, 2421, 2630
BsiEI CGRYCG 1 cut(s) 33
BsiHKAI GWGCWC 4 cut(s) 1473, 1548, 2094, 2896
BsiSI CCGG 6 cut(s) 33, 445, 2334, 2429, 2546, 2864
BslFI GGGAC 5 cut(s) 294, 1333, 1645, 1706, 2980
BslI CCNNNNNNNGG 7 cut(s) 433, 1324, 1436, 2285, 2417, 2428, 2714
BsmAI GTCTC 5 cut(s) 93, 561, 2039, 2516, 2592
BsmBI CGTCTC 1 cut(s) 2516
BsmFI GGGAC 5 cut(s) 294, 1333, 1645, 1706, 2980
BsmI GAATGC 1 cut(s) 2227
BsnI GGCC 4 cut(s) 1065, 1678, 2421, 2630
Bso31I GGTCTC 1 cut(s) 2592
Bsp119I TTCGAA 1 cut(s) 1076
Bsp1286I GDGCHC 4 cut(s) 1473, 1548, 2094, 2896
Bsp1407I TGTACA 1 cut(s) 714
Bsp19I CCATGG 1 cut(s) 1097
BspANI GGCC 4 cut(s) 1065, 1678, 2421, 2630
BspHI TCATGA 2 cut(s) 1477, 1726
BspLI GGNNCC 4 cut(s) 984, 2119, 2497, 2819
BspMAI CTGCAG 1 cut(s) 1185
BspPI GGATC 9 cut(s) 337, 882, 977, 990, 2112, 2125, 2778, 2812, 2825
BspQI GCTCTTC 1 cut(s) 2966
BspT104I TTCGAA 1 cut(s) 1076
BspTNI GGTCTC 1 cut(s) 2592
BsrDI GCAATG 2 cut(s) 190, 1646
BsrFI RCCGGY 1 cut(s) 32
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 8 cut(s) 115, 225, 288, 1208, 1679, 2246, 2564, 2986
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 5 cut(s) 18, 432, 1097, 1351, 1673
BssSI CACGAG 2 cut(s) 165, 1284
BssT1I CCWWGG 2 cut(s) 1097, 1351
Bst2BI CACGAG 2 cut(s) 165, 1284
Bst2UI CCWGG 1 cut(s) 20
Bst4CI ACNGT 5 cut(s) 320, 366, 1386, 1991, 2318
Bst6I CTCTTC 3 cut(s) 134, 815, 2966
BstAUI TGTACA 1 cut(s) 714
BstBAI YACGTR 1 cut(s) 2573
BstBI TTCGAA 1 cut(s) 1076
BstC8I GCNNGC 3 cut(s) 1569, 2163, 2363
BstDSI CCRYGG 2 cut(s) 432, 1097
BstHHI GCGC 1 cut(s) 2393
BstMAI GTCTC 5 cut(s) 93, 561, 2039, 2516, 2592
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 4 cut(s) 11, 1568, 2171, 2351
BstNI CCWGG 1 cut(s) 20
BstNSI RCATGY 6 cut(s) 106, 1571, 2285, 2545, 2587, 2908
BstSCI CCNGG 2 cut(s) 18, 2332
BstSFI CTRYAG 2 cut(s) 459, 1181
BstSLI GKGCMC 1 cut(s) 1548
BstV1I GCAGC 6 cut(s) 218, 886, 1340, 2158, 2491, 2925
BstV2I GAAGAC 3 cut(s) 812, 1387, 2102
BstX2I RGATCY 4 cut(s) 226, 982, 2117, 2817
BstYI RGATCY 4 cut(s) 226, 982, 2117, 2817
BsuRI GGCC 4 cut(s) 1065, 1678, 2421, 2630
BtgI CCRYGG 2 cut(s) 432, 1097
BtsIMutI CAGTG 1 cut(s) 108
Cac8I GCNNGC 3 cut(s) 1569, 2163, 2363
CciI TCATGA 2 cut(s) 1477, 1726
CfoI GCGC 1 cut(s) 2393
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 1 cut(s) 1315
CseI GACGC 2 cut(s) 1560, 2533
Csp6I GTAC 7 cut(s) 23, 332, 483, 715, 836, 2983, 2989
CviQI GTAC 7 cut(s) 23, 332, 483, 715, 836, 2983, 2989
DraI TTTAAA 1 cut(s) 2056
DraIII CACNNNGTG 1 cut(s) 2537
EaeI YGGCCR 2 cut(s) 2419, 2628
Eam1104I CTCTTC 3 cut(s) 134, 815, 2966
EarI CTCTTC 3 cut(s) 134, 815, 2966
Ecl136II GAGCTC 2 cut(s) 1471, 2092
Eco130I CCWWGG 2 cut(s) 1097, 1351
Eco24I GRGCYC 2 cut(s) 1473, 2094
Eco31I GGTCTC 1 cut(s) 2592
Eco32I GATATC 1 cut(s) 2078
Eco47I GGWCC 1 cut(s) 1315
Eco53kI GAGCTC 2 cut(s) 1471, 2092
Eco57I CTGAAG 2 cut(s) 38, 273
EcoICRI GAGCTC 2 cut(s) 1471, 2092
EcoRI GAATTC 1 cut(s) 1129
EcoRII CCWGG 1 cut(s) 18
EcoRV GATATC 1 cut(s) 2078
EcoT14I CCWWGG 2 cut(s) 1097, 1351
EcoT22I ATGCAT 2 cut(s) 1467, 2053
EcoT38I GRGCYC 2 cut(s) 1473, 2094
ErhI CCWWGG 2 cut(s) 1097, 1351
Esp3I CGTCTC 1 cut(s) 2516
FalI AAGNNNNNCTT 6 cut(s) 280, 312, 2180, 2212, 2247, 2279
FaqI GGGAC 5 cut(s) 294, 1333, 1645, 1706, 2980
FbaI TGATCA 1 cut(s) 1288
FblI GTMKAC 2 cut(s) 117, 642
Fnu4HI GCNGC 6 cut(s) 207, 900, 1329, 2172, 2505, 2914
FriOI GRGCYC 2 cut(s) 1473, 2094
Fsp4HI GCNGC 6 cut(s) 207, 900, 1329, 2172, 2505, 2914
FspBI CTAG 6 cut(s) 237, 416, 750, 839, 1067, 2031
FspI TGCGCA 1 cut(s) 2392
GlaI GCGC 1 cut(s) 2392
GluI GCNGC 6 cut(s) 207, 900, 1329, 2172, 2505, 2914
GsaI CCCAGC 2 cut(s) 612, 2289
GsuI CTGGAG 1 cut(s) 2880
HaeIII GGCC 4 cut(s) 1065, 1678, 2421, 2630
HapII CCGG 6 cut(s) 33, 445, 2334, 2429, 2546, 2864
HgaI GACGC 2 cut(s) 1560, 2533
HhaI GCGC 1 cut(s) 2393
Hin6I GCGC 1 cut(s) 2391
HinP1I GCGC 1 cut(s) 2391
HincII GTYRAC 6 cut(s) 67, 118, 316, 1994, 2028, 2931
HindII GTYRAC 6 cut(s) 67, 118, 316, 1994, 2028, 2931
HindIII AAGCTT 2 cut(s) 1750, 1922
HpaI GTTAAC 1 cut(s) 2028
HpaII CCGG 6 cut(s) 33, 445, 2334, 2429, 2546, 2864
HphI GGTGA 2 cut(s) 1454, 2857
Hpy188I TCNGA 9 cut(s) 131, 370, 792, 1674, 2082, 2231, 2597, 2813, 2889
Hpy99I CGWCG 2 cut(s) 46, 122
HpyCH4III ACNGT 5 cut(s) 320, 366, 1386, 1991, 2318
HpyCH4IV ACGT 2 cut(s) 41, 2572
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 1568, 2171, 2351
HpySE526I ACGT 2 cut(s) 41, 2572
HspAI GCGC 1 cut(s) 2391
Ksp22I TGATCA 1 cut(s) 1288
KspAI GTTAAC 1 cut(s) 2028
LguI GCTCTTC 1 cut(s) 2966
LmnI GCTCC 5 cut(s) 481, 912, 2089, 2209, 2899
Lsp1109I GCAGC 6 cut(s) 218, 886, 1340, 2158, 2491, 2925
LweI GCATC 7 cut(s) 23, 204, 238, 372, 640, 1141, 2769
MaeI CTAG 6 cut(s) 237, 416, 750, 839, 1067, 2031
MaeII ACGT 2 cut(s) 41, 2572
MaeIII GTNAC 9 cut(s) 406, 992, 1826, 2178, 2298, 2312, 2537, 2555, 2573
MflI RGATCY 4 cut(s) 226, 982, 2117, 2817
MhlI GDGCHC 4 cut(s) 1473, 1548, 2094, 2896
MlsI TGGCCA 2 cut(s) 2421, 2630
MluNI TGGCCA 2 cut(s) 2421, 2630
MlyI GAGTC 4 cut(s) 81, 334, 749, 1780
MmeI TCCRAC 4 cut(s) 302, 1342, 1357, 2187
Mox20I TGGCCA 2 cut(s) 2421, 2630
Mph1103I ATGCAT 2 cut(s) 1467, 2053
MroXI GAANNNNTTC 4 cut(s) 580, 760, 2043, 2406
MscI TGGCCA 2 cut(s) 2421, 2630
MseI TTAA 8 cut(s) 375, 1035, 1160, 1946, 1959, 2027, 2055, 2274
MslI CAYNNNNRTG 1 cut(s) 2665
Msp20I TGGCCA 2 cut(s) 2421, 2630
MspA1I CMGCKG 1 cut(s) 2504
MspI CCGG 6 cut(s) 33, 445, 2334, 2429, 2546, 2864
MspR9I CCNGG 2 cut(s) 20, 2334
Mva1269I GAATGC 1 cut(s) 2227
MvaI CCWGG 1 cut(s) 20
MwoI GCNNNNNNNGC 4 cut(s) 11, 1568, 2171, 2351
NciI CCSGG 1 cut(s) 2334
NcoI CCATGG 1 cut(s) 1097
NlaIV GGNNCC 4 cut(s) 984, 2119, 2497, 2819
NmeAIII GCCGAG 1 cut(s) 1543
NmuCI GTSAC 3 cut(s) 992, 2537, 2573
NsbI TGCGCA 1 cut(s) 2392
NsiI ATGCAT 2 cut(s) 1467, 2053
NspI RCATGY 6 cut(s) 106, 1571, 2285, 2545, 2587, 2908
NspV TTCGAA 1 cut(s) 1076
PaeI GCATGC 1 cut(s) 1571
PagI TCATGA 2 cut(s) 1477, 1726
PciI ACATGT 2 cut(s) 102, 2904
PciSI GCTCTTC 1 cut(s) 2966
PctI GAATGC 1 cut(s) 2227
PdmI GAANNNNTTC 4 cut(s) 580, 760, 2043, 2406
PfeI GAWTC 8 cut(s) 157, 500, 1030, 1799, 2134, 2153, 2612, 2848
PflFI GACNNNGTC 2 cut(s) 71, 1989
PflMI CCANNNNNTGG 2 cut(s) 1436, 2285
PfoI TCCNGGA 1 cut(s) 2332
PkrI GCNGC 6 cut(s) 208, 901, 1330, 2173, 2506, 2915
PleI GAGTC 4 cut(s) 80, 333, 748, 1779
PpsI GAGTC 4 cut(s) 80, 333, 748, 1779
Ppu21I YACGTR 1 cut(s) 2573
PscI ACATGT 2 cut(s) 102, 2904
Psp124BI GAGCTC 2 cut(s) 1473, 2094
Psp6I CCWGG 1 cut(s) 18
PspFI CCCAGC 2 cut(s) 608, 2285
PspGI CCWGG 1 cut(s) 18
PspN4I GGNNCC 4 cut(s) 984, 2119, 2497, 2819
PspPI GGNCC 1 cut(s) 1315
PstI CTGCAG 1 cut(s) 1185
PsuI RGATCY 4 cut(s) 226, 982, 2117, 2817
PsyI GACNNNGTC 2 cut(s) 71, 1989
PvuII CAGCTG 1 cut(s) 2504
RsaI GTAC 7 cut(s) 24, 333, 484, 716, 837, 2984, 2990
RsaNI GTAC 7 cut(s) 23, 332, 483, 715, 836, 2983, 2989
RseI CAYNNNNRTG 1 cut(s) 2665
SacI GAGCTC 2 cut(s) 1473, 2094
SalI GTCGAC 1 cut(s) 116
SapI GCTCTTC 1 cut(s) 2966
SaqAI TTAA 8 cut(s) 375, 1035, 1160, 1946, 1959, 2027, 2055, 2274
SatI GCNGC 6 cut(s) 207, 900, 1329, 2172, 2505, 2914
Sau96I GGNCC 1 cut(s) 1315
ScaI AGTACT 1 cut(s) 2990
SchI GAGTC 4 cut(s) 81, 334, 749, 1780
ScrFI CCNGG 2 cut(s) 20, 2334
SduI GDGCHC 4 cut(s) 1473, 1548, 2094, 2896
SfaNI GCATC 7 cut(s) 23, 204, 238, 372, 640, 1141, 2769
SfcI CTRYAG 2 cut(s) 459, 1181
SfuI TTCGAA 1 cut(s) 1076
SinI GGWCC 1 cut(s) 1315
SmiMI CAYNNNNRTG 1 cut(s) 2665
SmlI CTYRAG 7 cut(s) 737, 1753, 1907, 2093, 2138, 2366, 2435
SmoI CTYRAG 7 cut(s) 737, 1753, 1907, 2093, 2138, 2366, 2435
SpeI ACTAGT 2 cut(s) 236, 2030
SphI GCATGC 1 cut(s) 1571
SspI AATATT 1 cut(s) 1435
SspMI CTAG 6 cut(s) 237, 416, 750, 839, 1067, 2031
SstI GAGCTC 2 cut(s) 1473, 2094
StyD4I CCNGG 2 cut(s) 18, 2332
StyI CCWWGG 2 cut(s) 1097, 1351
TaaI ACNGT 5 cut(s) 320, 366, 1386, 1991, 2318
TaiI ACGT 2 cut(s) 44, 2575
TaqI TCGA 6 cut(s) 29, 117, 934, 1076, 1406, 2037
TatI WGTACW 4 cut(s) 482, 714, 835, 2988
TfiI GAWTC 8 cut(s) 157, 500, 1030, 1799, 2134, 2153, 2612, 2848
Tru1I TTAA 8 cut(s) 375, 1035, 1160, 1946, 1959, 2027, 2055, 2274
Tru9I TTAA 8 cut(s) 375, 1035, 1160, 1946, 1959, 2027, 2055, 2274
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 3 cut(s) 992, 2537, 2573
TseI GCWGC 6 cut(s) 206, 899, 1328, 2171, 2504, 2913
Tsp45I GTSAC 3 cut(s) 992, 2537, 2573
TspGWI ACGGA 2 cut(s) 215, 449
TspRI CASTG 1 cut(s) 115
Tth111I GACNNNGTC 2 cut(s) 71, 1989
Van91I CCANNNNNTGG 2 cut(s) 1436, 2285
VneI GTGCAC 1 cut(s) 1544
VpaK11BI GGWCC 1 cut(s) 1315
XapI RAATTY 7 cut(s) 80, 668, 942, 1129, 1730, 1765, 1810
XceI RCATGY 6 cut(s) 106, 1571, 2285, 2545, 2587, 2908
XmiI GTMKAC 2 cut(s) 117, 642
XmnI GAANNNNTTC 4 cut(s) 580, 760, 2043, 2406
XspI CTAG 6 cut(s) 237, 416, 750, 839, 1067, 2031
ZrmI AGTACT 1 cut(s) 2990
Zsp2I ATGCAT 2 cut(s) 1467, 2053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.