disease resistance
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 719 |
| Acc16I | TGCGCA | 1 cut(s) | 786 |
| Acc65I | GGTACC | 1 cut(s) | 1826 |
| AccB1I | GGYRCC | 2 cut(s) | 1826, 2088 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 142 |
| AccI | GTMKAC | 1 cut(s) | 1574 |
| AccIII | TCCGGA | 2 cut(s) | 769, 1778 |
| AciI | CCGC | 4 cut(s) | 321, 1824, 2295, 2367 |
| AclWI | GGATC | 5 cut(s) | 107, 1333, 2094, 2107, 2377 |
| AcoI | YGGCCR | 2 cut(s) | 678, 1125 |
| AcsI | RAATTY | 7 cut(s) | 162, 671, 1747, 1841, 2303, 2525, 2618 |
| AcuI | CTGAAG | 2 cut(s) | 746, 1377 |
| AdeI | CACNNNGTG | 1 cut(s) | 2396 |
| AfaI | GTAC | 8 cut(s) | 1000, 1412, 1682, 1759, 1828, 2377, 2642, 2771 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 142, 556, 1120, 1348, 1469, 2261, 2393, 2708 |
| AflII | CTTAAG | 2 cut(s) | 1735, 2657 |
| AflIII | ACRYGT | 1 cut(s) | 1558 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1886 |
| AhlI | ACTAGT | 1 cut(s) | 2012 |
| AjiI | CACGTC | 1 cut(s) | 1561 |
| AjnI | CCWGG | 3 cut(s) | 549, 1462, 2701 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2162, 2194 |
| Alw21I | GWGCWC | 4 cut(s) | 109, 513, 1430, 2076 |
| Alw26I | GTCTC | 4 cut(s) | 713, 1157, 1522, 2819 |
| AlwI | GGATC | 5 cut(s) | 107, 1333, 2094, 2107, 2377 |
| Ama87I | CYCGRG | 2 cut(s) | 932, 1374 |
| Aor13HI | TCCGGA | 2 cut(s) | 769, 1778 |
| AoxI | GGCC | 6 cut(s) | 317, 452, 678, 1084, 1111, 1125 |
| ApeKI | GCWGC | 7 cut(s) | 470, 541, 926, 1352, 1915, 2153, 2651 |
| ApoI | RAATTY | 7 cut(s) | 162, 671, 1747, 1841, 2303, 2525, 2618 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2406, 2438 |
| Asp718I | GGTACC | 1 cut(s) | 1826 |
| AspLEI | GCGC | 1 cut(s) | 787 |
| AspS9I | GGNCC | 2 cut(s) | 318, 1434 |
| AsuC2I | CCSGG | 3 cut(s) | 933, 934, 1851 |
| AsuHPI | GGTGA | 6 cut(s) | 389, 406, 709, 724, 1802, 2791 |
| AvaI | CYCGRG | 2 cut(s) | 932, 1374 |
| AvaII | GGWCC | 1 cut(s) | 1434 |
| BalI | TGGCCA | 2 cut(s) | 680, 1127 |
| BamHI | GGATCC | 1 cut(s) | 2099 |
| BanI | GGYRCC | 2 cut(s) | 1826, 2088 |
| BanII | GRGCYC | 2 cut(s) | 513, 2076 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1966, 1998 |
| BauI | CACGAG | 2 cut(s) | 108, 857 |
| BbsI | GAAGAC | 2 cut(s) | 629, 767 |
| Bbv12I | GWGCWC | 4 cut(s) | 109, 513, 1430, 2076 |
| BbvI | GCAGC | 7 cut(s) | 457, 553, 938, 1364, 1902, 2140, 2638 |
| BciT130I | CCWGG | 3 cut(s) | 551, 1464, 2703 |
| BciVI | GTATCC | 1 cut(s) | 1540 |
| BclI | TGATCA | 4 cut(s) | 652, 988, 1833, 2736 |
| BcnI | CCSGG | 3 cut(s) | 933, 934, 1851 |
| BcoDI | GTCTC | 4 cut(s) | 713, 1157, 1522, 2819 |
| BcuI | ACTAGT | 1 cut(s) | 2012 |
| BfaI | CTAG | 5 cut(s) | 390, 641, 863, 2013, 2648 |
| BfmI | CTRYAG | 4 cut(s) | 24, 924, 1088, 2773 |
| BfrI | CTTAAG | 2 cut(s) | 1735, 2657 |
| BfuI | GTATCC | 1 cut(s) | 1540 |
| BglII | AGATCT | 2 cut(s) | 920, 2213 |
| BisI | GCNGC | 7 cut(s) | 471, 542, 927, 1353, 1916, 2154, 2652 |
| BlsI | GCNGC | 7 cut(s) | 472, 543, 928, 1354, 1917, 2155, 2653 |
| BmcAI | AGTACT | 2 cut(s) | 1412, 2771 |
| Bme1390I | CCNGG | 6 cut(s) | 551, 933, 934, 1464, 1851, 2703 |
| Bme18I | GGWCC | 1 cut(s) | 1434 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1886 |
| BmeT110I | CYCGRG | 2 cut(s) | 932, 1374 |
| BmgBI | CACGTC | 1 cut(s) | 1561 |
| BmgT120I | GGNCC | 2 cut(s) | 318, 1434 |
| BmiI | GGNNCC | 5 cut(s) | 768, 1828, 2090, 2101, 2205 |
| BmrFI | CCNGG | 6 cut(s) | 551, 933, 934, 1464, 1851, 2703 |
| BmsI | GCATC | 5 cut(s) | 51, 172, 244, 889, 1773 |
| BpiI | GAAGAC | 2 cut(s) | 629, 767 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 442, 474, 1744, 1776 |
| BpmI | CTGGAG | 1 cut(s) | 572 |
| Bpu10I | CCTNAGC | 2 cut(s) | 2148, 2685 |
| BpuEI | CTTGAG | 7 cut(s) | 767, 1078, 1414, 1928, 2060, 2141, 2261 |
| BpuMI | CCSGG | 3 cut(s) | 933, 934, 1851 |
| BsaBI | GATNNNNATC | 4 cut(s) | 224, 657, 882, 1221 |
| BsaJI | CCNNGG | 6 cut(s) | 449, 932, 2095, 2207, 2451, 2702 |
| BsaWI | WCCGGW | 2 cut(s) | 769, 1778 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 545, 575 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 142, 556, 1120, 1348, 1469, 2261, 2393, 2708 |
| Bse1I | ACTGG | 3 cut(s) | 43, 777, 1235 |
| Bse3DI | GCAATG | 1 cut(s) | 2204 |
| Bse8I | GATNNNNATC | 4 cut(s) | 224, 657, 882, 1221 |
| BseAI | TCCGGA | 2 cut(s) | 769, 1778 |
| BseBI | CCWGG | 3 cut(s) | 551, 1464, 2703 |
| BseDI | CCNNGG | 6 cut(s) | 449, 932, 2095, 2207, 2451, 2702 |
| BseJI | GATNNNNATC | 4 cut(s) | 224, 657, 882, 1221 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 142, 556, 1120, 1348, 1469, 2261, 2393, 2708 |
| BseMI | GCAATG | 1 cut(s) | 2204 |
| BseMII | CTCAG | 8 cut(s) | 320, 447, 494, 1084, 1533, 1911, 2676, 2820 |
| BseNI | ACTGG | 3 cut(s) | 43, 777, 1235 |
| BseRI | GAGGAG | 4 cut(s) | 465, 468, 1925, 2084 |
| BseXI | GCAGC | 7 cut(s) | 457, 553, 938, 1364, 1902, 2140, 2638 |
| BseYI | CCCAGC | 1 cut(s) | 636 |
| Bsh1285I | CGRYCG | 1 cut(s) | 94 |
| BshFI | GGCC | 6 cut(s) | 319, 454, 680, 1086, 1113, 1127 |
| BshNI | GGYRCC | 2 cut(s) | 1826, 2088 |
| BsiEI | CGRYCG | 1 cut(s) | 94 |
| BsiHKAI | GWGCWC | 4 cut(s) | 109, 513, 1430, 2076 |
| BsiHKCI | CYCGRG | 2 cut(s) | 932, 1374 |
| BsiSI | CCGG | 5 cut(s) | 770, 933, 1585, 1779, 1850 |
| BslFI | GGGAC | 3 cut(s) | 49, 851, 2810 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 142, 556, 1120, 1348, 1469, 2261, 2393, 2708 |
| BsmAI | GTCTC | 4 cut(s) | 713, 1157, 1522, 2819 |
| BsmBI | CGTCTC | 1 cut(s) | 1157 |
| BsmFI | GGGAC | 3 cut(s) | 49, 851, 2810 |
| BsmI | GAATGC | 2 cut(s) | 1588, 2109 |
| BsnI | GGCC | 6 cut(s) | 319, 454, 680, 1086, 1113, 1127 |
| BsoBI | CYCGRG | 2 cut(s) | 932, 1374 |
| Bsp1286I | GDGCHC | 4 cut(s) | 109, 513, 1430, 2076 |
| Bsp13I | TCCGGA | 2 cut(s) | 769, 1778 |
| Bsp1407I | TGTACA | 2 cut(s) | 2375, 2640 |
| BspACI | CCGC | 4 cut(s) | 321, 1824, 2295, 2367 |
| BspANI | GGCC | 6 cut(s) | 319, 454, 680, 1086, 1113, 1127 |
| BspCNI | CTCAG | 8 cut(s) | 319, 446, 493, 1083, 1534, 1912, 2677, 2821 |
| BspEI | TCCGGA | 2 cut(s) | 769, 1778 |
| BspHI | TCATGA | 1 cut(s) | 1498 |
| BspLI | GGNNCC | 5 cut(s) | 768, 1828, 2090, 2101, 2205 |
| BspMAI | CTGCAG | 1 cut(s) | 928 |
| BspPI | GGATC | 5 cut(s) | 107, 1333, 2094, 2107, 2377 |
| BspT107I | GGYRCC | 2 cut(s) | 1826, 2088 |
| BspTI | CTTAAG | 2 cut(s) | 1735, 2657 |
| BsrDI | GCAATG | 1 cut(s) | 2204 |
| BsrGI | TGTACA | 2 cut(s) | 2375, 2640 |
| BsrI | ACTGG | 3 cut(s) | 43, 777, 1235 |
| BssECI | CCNNGG | 6 cut(s) | 449, 932, 2095, 2207, 2451, 2702 |
| BssSI | CACGAG | 2 cut(s) | 108, 857 |
| BssT1I | CCWWGG | 1 cut(s) | 2207 |
| Bst2BI | CACGAG | 2 cut(s) | 108, 857 |
| Bst2UI | CCWGG | 3 cut(s) | 551, 1464, 2703 |
| Bst4CI | ACNGT | 8 cut(s) | 21, 766, 1347, 2032, 2224, 2452, 2774, 2850 |
| Bst6I | CTCTTC | 5 cut(s) | 1073, 1305, 1399, 1626, 2412 |
| BstAFI | CTTAAG | 2 cut(s) | 1735, 2657 |
| BstAUI | TGTACA | 2 cut(s) | 2375, 2640 |
| BstC8I | GCNNGC | 6 cut(s) | 321, 409, 456, 1266, 2339, 2541 |
| BstDSI | CCRYGG | 2 cut(s) | 2095, 2451 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1467 |
| BstHHI | GCGC | 1 cut(s) | 787 |
| BstMAI | GTCTC | 4 cut(s) | 713, 1157, 1522, 2819 |
| BstMCI | CGRYCG | 1 cut(s) | 94 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 316, 2153 |
| BstNI | CCWGG | 3 cut(s) | 551, 1464, 2703 |
| BstNSI | RCATGY | 2 cut(s) | 874, 1693 |
| BstSCI | CCNGG | 6 cut(s) | 549, 931, 932, 1462, 1849, 2701 |
| BstSFI | CTRYAG | 4 cut(s) | 24, 924, 1088, 2773 |
| BstV1I | GCAGC | 7 cut(s) | 457, 553, 938, 1364, 1902, 2140, 2638 |
| BstV2I | GAAGAC | 2 cut(s) | 629, 767 |
| BstX2I | RGATCY | 5 cut(s) | 112, 920, 2099, 2213, 2369 |
| BstYI | RGATCY | 5 cut(s) | 112, 920, 2099, 2213, 2369 |
| BsuI | GTATCC | 1 cut(s) | 1540 |
| BsuRI | GGCC | 6 cut(s) | 319, 454, 680, 1086, 1113, 1127 |
| BtgI | CCRYGG | 2 cut(s) | 2095, 2451 |
| BtrI | CACGTC | 1 cut(s) | 1561 |
| BtsIMutI | CAGTG | 2 cut(s) | 1078, 1242 |
| Cac8I | GCNNGC | 6 cut(s) | 321, 409, 456, 1266, 2339, 2541 |
| CciI | TCATGA | 1 cut(s) | 1498 |
| CfoI | GCGC | 1 cut(s) | 787 |
| Cfr13I | GGNCC | 2 cut(s) | 318, 1434 |
| Cfr9I | CCCGGG | 1 cut(s) | 932 |
| CseI | GACGC | 1 cut(s) | 2559 |
| Csp6I | GTAC | 8 cut(s) | 999, 1411, 1681, 1758, 1827, 2376, 2641, 2770 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1545, 1580 |
| CviQI | GTAC | 8 cut(s) | 999, 1411, 1681, 1758, 1827, 2376, 2641, 2770 |
| DraIII | CACNNNGTG | 1 cut(s) | 2396 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 719 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1886 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 719 |
| EaeI | YGGCCR | 2 cut(s) | 678, 1125 |
| Eam1104I | CTCTTC | 5 cut(s) | 1073, 1305, 1399, 1626, 2412 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1886 |
| EarI | CTCTTC | 5 cut(s) | 1073, 1305, 1399, 1626, 2412 |
| Ecl136II | GAGCTC | 2 cut(s) | 511, 2074 |
| Eco130I | CCWWGG | 1 cut(s) | 2207 |
| Eco147I | AGGCCT | 2 cut(s) | 454, 1113 |
| Eco24I | GRGCYC | 2 cut(s) | 513, 2076 |
| Eco47I | GGWCC | 1 cut(s) | 1434 |
| Eco53kI | GAGCTC | 2 cut(s) | 511, 2074 |
| Eco57I | CTGAAG | 2 cut(s) | 746, 1377 |
| Eco88I | CYCGRG | 2 cut(s) | 932, 1374 |
| EcoICRI | GAGCTC | 2 cut(s) | 511, 2074 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1467 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1434 |
| EcoRII | CCWGG | 3 cut(s) | 549, 1462, 2701 |
| EcoT14I | CCWWGG | 1 cut(s) | 2207 |
| EcoT22I | ATGCAT | 1 cut(s) | 1488 |
| EcoT38I | GRGCYC | 2 cut(s) | 513, 2076 |
| ErhI | CCWWGG | 1 cut(s) | 2207 |
| Esp3I | CGTCTC | 1 cut(s) | 1157 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 2162, 2194, 2223, 2255 |
| FaqI | GGGAC | 3 cut(s) | 49, 851, 2810 |
| FauI | CCCGC | 1 cut(s) | 328 |
| FbaI | TGATCA | 4 cut(s) | 652, 988, 1833, 2736 |
| FblI | GTMKAC | 1 cut(s) | 1574 |
| Fnu4HI | GCNGC | 7 cut(s) | 471, 542, 927, 1353, 1916, 2154, 2652 |
| FriOI | GRGCYC | 2 cut(s) | 513, 2076 |
| Fsp4HI | GCNGC | 7 cut(s) | 471, 542, 927, 1353, 1916, 2154, 2652 |
| FspBI | CTAG | 5 cut(s) | 390, 641, 863, 2013, 2648 |
| FspI | TGCGCA | 1 cut(s) | 786 |
| GlaI | GCGC | 1 cut(s) | 786 |
| GluI | GCNGC | 7 cut(s) | 471, 542, 927, 1353, 1916, 2154, 2652 |
| GsaI | CCCAGC | 1 cut(s) | 640 |
| GsuI | CTGGAG | 1 cut(s) | 572 |
| HaeIII | GGCC | 6 cut(s) | 319, 454, 680, 1086, 1113, 1127 |
| HapII | CCGG | 5 cut(s) | 770, 933, 1585, 1779, 1850 |
| HgaI | GACGC | 1 cut(s) | 2559 |
| HhaI | GCGC | 1 cut(s) | 787 |
| Hin6I | GCGC | 1 cut(s) | 785 |
| HinP1I | GCGC | 1 cut(s) | 785 |
| HincII | GTYRAC | 2 cut(s) | 2010, 2227 |
| HindII | GTYRAC | 2 cut(s) | 2010, 2227 |
| HindIII | AAGCTT | 3 cut(s) | 2117, 2339, 2594 |
| HpaI | GTTAAC | 1 cut(s) | 2010 |
| HpaII | CCGG | 5 cut(s) | 770, 933, 1585, 1779, 1850 |
| HphI | GGTGA | 6 cut(s) | 389, 406, 709, 724, 1802, 2791 |
| Hpy166II | GTNNAC | 7 cut(s) | 753, 802, 1575, 2010, 2227, 2378, 2399 |
| Hpy8I | GTNNAC | 7 cut(s) | 753, 802, 1575, 2010, 2227, 2378, 2399 |
| Hpy99I | CGWCG | 1 cut(s) | 716 |
| HpyCH4III | ACNGT | 8 cut(s) | 21, 766, 1347, 2032, 2224, 2452, 2774, 2850 |
| HpyCH4IV | ACGT | 2 cut(s) | 66, 1560 |
| HpyCH4V | TGCA | 8 cut(s) | 257, 926, 1486, 2109, 2197, 2337, 2491, 2543 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 316, 2153 |
| HpySE526I | ACGT | 2 cut(s) | 66, 1560 |
| HspAI | GCGC | 1 cut(s) | 785 |
| Kpn2I | TCCGGA | 2 cut(s) | 769, 1778 |
| KpnI | GGTACC | 1 cut(s) | 1830 |
| Ksp22I | TGATCA | 4 cut(s) | 652, 988, 1833, 2736 |
| KspAI | GTTAAC | 1 cut(s) | 2010 |
| LmnI | GCTCC | 5 cut(s) | 138, 478, 936, 1912, 2071 |
| Lsp1109I | GCAGC | 7 cut(s) | 457, 553, 938, 1364, 1902, 2140, 2638 |
| LweI | GCATC | 5 cut(s) | 51, 172, 244, 889, 1773 |
| MaeI | CTAG | 5 cut(s) | 390, 641, 863, 2013, 2648 |
| MaeII | ACGT | 2 cut(s) | 66, 1560 |
| MfeI | CAATTG | 1 cut(s) | 892 |
| MflI | RGATCY | 5 cut(s) | 112, 920, 2099, 2213, 2369 |
| MhlI | GDGCHC | 4 cut(s) | 109, 513, 1430, 2076 |
| MlsI | TGGCCA | 2 cut(s) | 680, 1127 |
| MluNI | TGGCCA | 2 cut(s) | 680, 1127 |
| MlyI | GAGTC | 5 cut(s) | 271, 758, 797, 949, 1875 |
| MmeI | TCCRAC | 4 cut(s) | 123, 592, 1366, 1608 |
| Mox20I | TGGCCA | 2 cut(s) | 680, 1127 |
| Mph1103I | ATGCAT | 1 cut(s) | 1488 |
| MroI | TCCGGA | 2 cut(s) | 769, 1778 |
| MscI | TGGCCA | 2 cut(s) | 680, 1127 |
| MslI | CAYNNNNRTG | 4 cut(s) | 177, 944, 1815, 1985 |
| Msp20I | TGGCCA | 2 cut(s) | 680, 1127 |
| MspA1I | CMGCKG | 2 cut(s) | 581, 1355 |
| MspCI | CTTAAG | 2 cut(s) | 1735, 2657 |
| MspI | CCGG | 5 cut(s) | 770, 933, 1585, 1779, 1850 |
| MspR9I | CCNGG | 6 cut(s) | 551, 933, 934, 1464, 1851, 2703 |
| MunI | CAATTG | 1 cut(s) | 892 |
| Mva1269I | GAATGC | 2 cut(s) | 1588, 2109 |
| MvaI | CCWGG | 3 cut(s) | 551, 1464, 2703 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 316, 2153 |
| NciI | CCSGG | 3 cut(s) | 933, 934, 1851 |
| NlaIV | GGNNCC | 5 cut(s) | 768, 1828, 2090, 2101, 2205 |
| NmeAIII | GCCGAG | 1 cut(s) | 342 |
| NmuCI | GTSAC | 4 cut(s) | 1808, 1978, 2344, 2750 |
| NsbI | TGCGCA | 1 cut(s) | 786 |
| NsiI | ATGCAT | 1 cut(s) | 1488 |
| NspI | RCATGY | 2 cut(s) | 874, 1693 |
| PaeR7I | CTCGAG | 1 cut(s) | 1374 |
| PagI | TCATGA | 1 cut(s) | 1498 |
| PceI | AGGCCT | 2 cut(s) | 454, 1113 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 183 |
| PctI | GAATGC | 2 cut(s) | 1588, 2109 |
| PfeI | GAWTC | 8 cut(s) | 773, 883, 1147, 1183, 1459, 1616, 2353, 2842 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 142 |
| PfoI | TCCNGGA | 1 cut(s) | 549 |
| PkrI | GCNGC | 7 cut(s) | 472, 543, 928, 1354, 1917, 2155, 2653 |
| PleI | GAGTC | 5 cut(s) | 271, 757, 797, 949, 1875 |
| PpsI | GAGTC | 5 cut(s) | 271, 757, 797, 949, 1875 |
| PpuMI | RGGWCCY | 1 cut(s) | 1434 |
| Psp124BI | GAGCTC | 2 cut(s) | 513, 2076 |
| Psp5II | RGGWCCY | 1 cut(s) | 1434 |
| Psp6I | CCWGG | 3 cut(s) | 549, 1462, 2701 |
| PspFI | CCCAGC | 1 cut(s) | 636 |
| PspGI | CCWGG | 3 cut(s) | 549, 1462, 2701 |
| PspN4I | GGNNCC | 5 cut(s) | 768, 1828, 2090, 2101, 2205 |
| PspPI | GGNCC | 2 cut(s) | 318, 1434 |
| PspPPI | RGGWCCY | 1 cut(s) | 1434 |
| PspXI | VCTCGAGB | 1 cut(s) | 1374 |
| PstI | CTGCAG | 1 cut(s) | 928 |
| PsuI | RGATCY | 5 cut(s) | 112, 920, 2099, 2213, 2369 |
| PvuII | CAGCTG | 2 cut(s) | 581, 1355 |
| RsaI | GTAC | 8 cut(s) | 1000, 1412, 1682, 1759, 1828, 2377, 2642, 2771 |
| RsaNI | GTAC | 8 cut(s) | 999, 1411, 1681, 1758, 1827, 2376, 2641, 2770 |
| RseI | CAYNNNNRTG | 4 cut(s) | 177, 944, 1815, 1985 |
| SacI | GAGCTC | 2 cut(s) | 513, 2076 |
| SatI | GCNGC | 7 cut(s) | 471, 542, 927, 1353, 1916, 2154, 2652 |
| Sau96I | GGNCC | 2 cut(s) | 318, 1434 |
| ScaI | AGTACT | 2 cut(s) | 1412, 2771 |
| SchI | GAGTC | 5 cut(s) | 271, 758, 797, 949, 1875 |
| ScrFI | CCNGG | 6 cut(s) | 551, 933, 934, 1464, 1851, 2703 |
| SduI | GDGCHC | 4 cut(s) | 109, 513, 1430, 2076 |
| SfaNI | GCATC | 5 cut(s) | 51, 172, 244, 889, 1773 |
| SfcI | CTRYAG | 4 cut(s) | 24, 924, 1088, 2773 |
| Sfr274I | CTCGAG | 1 cut(s) | 1374 |
| SinI | GGWCC | 1 cut(s) | 1434 |
| SlaI | CTCGAG | 1 cut(s) | 1374 |
| SmaI | CCCGGG | 1 cut(s) | 934 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 177, 944, 1815, 1985 |
| SpeI | ACTAGT | 1 cut(s) | 2012 |
| SseBI | AGGCCT | 2 cut(s) | 454, 1113 |
| SsiI | CCGC | 4 cut(s) | 321, 1824, 2295, 2367 |
| SspMI | CTAG | 5 cut(s) | 390, 641, 863, 2013, 2648 |
| SstI | GAGCTC | 2 cut(s) | 513, 2076 |
| StuI | AGGCCT | 2 cut(s) | 454, 1113 |
| StyD4I | CCNGG | 6 cut(s) | 549, 931, 932, 1462, 1849, 2701 |
| StyI | CCWWGG | 1 cut(s) | 2207 |
| TaaI | ACNGT | 8 cut(s) | 21, 766, 1347, 2032, 2224, 2452, 2774, 2850 |
| TaiI | ACGT | 2 cut(s) | 69, 1563 |
| TaqI | TCGA | 9 cut(s) | 12, 94, 177, 228, 460, 711, 958, 1375, 2692 |
| TaqII | GACCGA | 1 cut(s) | 80 |
| TatI | WGTACW | 5 cut(s) | 998, 1410, 2375, 2640, 2769 |
| TfiI | GAWTC | 8 cut(s) | 773, 883, 1147, 1183, 1459, 1616, 2353, 2842 |
| TscAI | CASTG | 2 cut(s) | 1078, 1242 |
| TseFI | GTSAC | 4 cut(s) | 1808, 1978, 2344, 2750 |
| TseI | GCWGC | 7 cut(s) | 470, 541, 926, 1352, 1915, 2153, 2651 |
| Tsp45I | GTSAC | 4 cut(s) | 1808, 1978, 2344, 2750 |
| TspDTI | ATGAA | 7 cut(s) | 963, 1483, 1487, 1494, 1620, 2366, 2450 |
| TspGWI | ACGGA | 2 cut(s) | 2084, 2850 |
| TspMI | CCCGGG | 1 cut(s) | 932 |
| TspRI | CASTG | 2 cut(s) | 1078, 1242 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 142 |
| Vha464I | CTTAAG | 2 cut(s) | 1735, 2657 |
| VpaK11BI | GGWCC | 1 cut(s) | 1434 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1467 |
| XapI | RAATTY | 7 cut(s) | 162, 671, 1747, 1841, 2303, 2525, 2618 |
| XceI | RCATGY | 2 cut(s) | 874, 1693 |
| XhoI | CTCGAG | 1 cut(s) | 1374 |
| XmaI | CCCGGG | 1 cut(s) | 932 |
| XmiI | GTMKAC | 1 cut(s) | 1574 |
| XspI | CTAG | 5 cut(s) | 390, 641, 863, 2013, 2648 |
| ZrmI | AGTACT | 2 cut(s) | 1412, 2771 |
| Zsp2I | ATGCAT | 1 cut(s) | 1488 |