Rroxscaffold_5G00346070

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001339
Physical Location & Seq
Forward (+)
16737645 .. 16743047
5403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00346070.1

Sequence Viewer

Length: 738 bp
ATGAAGAATGGTCAAGATGATACACATACCAATAACAAGACTCCAAAAAAGGTTTTTGAACTATCAAGGAAGGAGGCTAAAGCATCGCCATCGTGGGTGGCAGGTTTTGCATTGTTCTGTTATATGGCCCACATAGTGGGTTCGTCGTTGACGGGTGATGTCTGTCTCCCACCTCTGATAAATTTTGGTCTCAGTGGTATGGTGTGTCTAGAGGGACCTAGAAAGTGGTGGTCCGGGATCGACGATGATCTCGAGCGGTTGGACGAGTTTTGGCTGAAAATTCAGTCTCCGATCGACGACGTTGAAAACAAGCACTCTATGTCCAAAGCCTCGCAATCCGTTAATCGAAGATTGCAAGGCCAAGACCTGCTCGAAGTTGCCCCATCTCTTCCCGTCGCTCGTTCGCAATCGAATGAAGGAGGGAATAGATCAGATCCGGAAGTTGTTGGCCCCTCAAGCCGACGCGAAGCAGAGAATGTTCATGTCATCCCAATAGTGGGTATGGGTGGCATCGGAAAAACAACACTCGCTCAACATGTCTACAATGATTCGCAGGTGGTCCGCGAGTTTGACTTGAGAATATGGGTAAGCGTTTCTTCAGATTTTGATTTGGTTAAAATTACTAAATCAATCCCGGAGTCCTCCATAATCAATTCTACCGATGACAATGACTCGCCTCATGCCCAACATACACATCAAGATCGGGGAGAGGTCGTGAAACTCGGTGAAGGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

245

Amino Acids

26.96

Weight (kDa)

5.48

Isoelectric Point (pI)

49.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 157 - 226 1.3e-15 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 544
Acc36I ACCTGC 3 cut(s) 92, 375, 544
AccB7I CCANNNNNTGG 1 cut(s) 136
AccBSI CCGCTC 1 cut(s) 256
AccI GTMKAC 1 cut(s) 540
AccII CGCG 2 cut(s) 465, 564
AccIII TCCGGA 1 cut(s) 436
AciI CCGC 2 cut(s) 256, 562
AclWI GGATC 2 cut(s) 245, 428
AcsI RAATTY 2 cut(s) 181, 279
AcuI CTGAAG 1 cut(s) 582
AdeI CACNNNGTG 1 cut(s) 136
AfiI CCNNNNNNNGG 3 cut(s) 136, 496, 497
AflIII ACRYGT 1 cut(s) 535
AgsI TTSAA 2 cut(s) 59, 305
AluBI AGCT 1 cut(s) 734
AluI AGCT 1 cut(s) 734
Alw26I GTCTC 3 cut(s) 170, 194, 291
AlwI GGATC 2 cut(s) 245, 428
Ama87I CYCGRG 1 cut(s) 251
Aor13HI TCCGGA 1 cut(s) 436
AoxI GGCC 3 cut(s) 126, 358, 448
ApoI RAATTY 2 cut(s) 181, 279
AspS9I GGNCC 5 cut(s) 127, 215, 231, 449, 559
AsuC2I CCSGG 2 cut(s) 235, 635
AsuHPI GGTGA 2 cut(s) 167, 737
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 3 cut(s) 215, 231, 559
BccI CCATC 2 cut(s) 97, 391
BcgI CGANNNNNNTGC 2 cut(s) 72, 106
BcnI CCSGG 2 cut(s) 235, 635
BcoDI GTCTC 3 cut(s) 170, 194, 291
BfaI CTAG 2 cut(s) 209, 219
BfuAI ACCTGC 3 cut(s) 92, 375, 544
Bme1390I CCNGG 2 cut(s) 235, 635
Bme18I GGWCC 3 cut(s) 215, 231, 559
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 5 cut(s) 127, 215, 231, 449, 559
BmiI GGNNCC 2 cut(s) 216, 451
BmrFI CCNGG 2 cut(s) 235, 635
BmsI GCATC 2 cut(s) 92, 519
BpuEI CTTGAG 2 cut(s) 439, 595
BpuMI CCSGG 2 cut(s) 235, 635
BsaI GGTCTC 1 cut(s) 194
BsaWI WCCGGW 1 cut(s) 436
BsaXI ACNNNNNCTCC 2 cut(s) 699, 729
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 496, 497
BseAI TCCGGA 1 cut(s) 436
BseGI GGATG 1 cut(s) 486
BseLI CCNNNNNNNGG 3 cut(s) 136, 496, 497
BseMII CTCAG 1 cut(s) 205
Bsh1236I CGCG 2 cut(s) 465, 564
Bsh1285I CGRYCG 1 cut(s) 294
BshFI GGCC 3 cut(s) 128, 360, 450
BsiEI CGRYCG 1 cut(s) 294
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 3 cut(s) 234, 437, 635
BslFI GGGAC 1 cut(s) 228
BslI CCNNNNNNNGG 3 cut(s) 136, 496, 497
BsmAI GTCTC 3 cut(s) 170, 194, 291
BsmFI GGGAC 1 cut(s) 228
BsnI GGCC 3 cut(s) 128, 360, 450
Bso31I GGTCTC 1 cut(s) 194
BsoBI CYCGRG 1 cut(s) 251
Bsp13I TCCGGA 1 cut(s) 436
Bsp143I GATC 6 cut(s) 237, 247, 291, 428, 433, 700
BspACI CCGC 2 cut(s) 256, 562
BspANI GGCC 3 cut(s) 128, 360, 450
BspCNI CTCAG 1 cut(s) 204
BspEI TCCGGA 1 cut(s) 436
BspFNI CGCG 2 cut(s) 465, 564
BspLI GGNNCC 2 cut(s) 216, 451
BspMI ACCTGC 3 cut(s) 92, 375, 544
BspPI GGATC 2 cut(s) 245, 428
BspTNI GGTCTC 1 cut(s) 194
BsrBI CCGCTC 1 cut(s) 256
BssMI GATC 6 cut(s) 237, 247, 291, 428, 433, 700
Bst6I CTCTTC 1 cut(s) 393
BstAPI GCANNNNNTGC 1 cut(s) 107
BstDEI CTNAG 1 cut(s) 191
BstF5I GGATG 1 cut(s) 486
BstFNI CGCG 2 cut(s) 465, 564
BstKTI GATC 6 cut(s) 240, 250, 294, 431, 436, 703
BstMAI GTCTC 3 cut(s) 170, 194, 291
BstMBI GATC 6 cut(s) 237, 247, 291, 428, 433, 700
BstMCI CGRYCG 1 cut(s) 294
BstMWI GCNNNNNNNGC 2 cut(s) 107, 456
BstNSI RCATGY 1 cut(s) 539
BstSCI CCNGG 2 cut(s) 233, 633
BstUI CGCG 2 cut(s) 465, 564
BstX2I RGATCY 1 cut(s) 433
BstYI RGATCY 1 cut(s) 433
BsuRI GGCC 3 cut(s) 128, 360, 450
BtgZI GCGATG 1 cut(s) 69
BtsCI GGATG 1 cut(s) 486
BtsIMutI CAGTG 1 cut(s) 199
BveI ACCTGC 3 cut(s) 92, 375, 544
Cfr13I GGNCC 5 cut(s) 127, 215, 231, 449, 559
CseI GACGC 1 cut(s) 471
CviAII CATG 3 cut(s) 482, 536, 680
CviJI RGCY 8 cut(s) 77, 128, 274, 329, 360, 450, 459, 734
CviKI_1 RGCY 8 cut(s) 77, 128, 274, 329, 360, 450, 459, 734
DdeI CTNAG 1 cut(s) 191
DpnI GATC 6 cut(s) 239, 249, 293, 430, 435, 702
DpnII GATC 6 cut(s) 237, 247, 291, 428, 433, 700
DraIII CACNNNGTG 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
Eco31I GGTCTC 1 cut(s) 194
Eco47I GGWCC 3 cut(s) 215, 231, 559
Eco57I CTGAAG 1 cut(s) 582
Eco88I CYCGRG 1 cut(s) 251
EcoO109I RGGNCCY 1 cut(s) 215
FaeI CATG 3 cut(s) 485, 539, 683
FalI AAGNNNNNCTT 2 cut(s) 580, 612
FaqI GGGAC 1 cut(s) 228
FatI CATG 3 cut(s) 481, 535, 679
FblI GTMKAC 1 cut(s) 540
FokI GGATG 1 cut(s) 473
FspBI CTAG 2 cut(s) 209, 219
HaeIII GGCC 3 cut(s) 128, 360, 450
HapII CCGG 3 cut(s) 234, 437, 635
HgaI GACGC 1 cut(s) 471
Hin1II CATG 3 cut(s) 485, 539, 683
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HinfI GANTC 4 cut(s) 40, 548, 638, 671
HpaII CCGG 3 cut(s) 234, 437, 635
HphI GGTGA 2 cut(s) 167, 737
Hpy166II GTNNAC 2 cut(s) 150, 541
Hpy188I TCNGA 5 cut(s) 177, 291, 433, 515, 601
Hpy188III TCNNGA 6 cut(s) 14, 209, 251, 437, 698, 715
Hpy8I GTNNAC 2 cut(s) 150, 541
Hpy99I CGWCG 6 cut(s) 148, 245, 299, 302, 398, 465
HpyAV CCTTC 3 cut(s) 64, 410, 722
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 2 cut(s) 110, 355
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 456
HpyF3I CTNAG 1 cut(s) 191
HpySE526I ACGT 1 cut(s) 300
Hsp92II CATG 3 cut(s) 485, 539, 683
Kpn2I TCCGGA 1 cut(s) 436
Kzo9I GATC 6 cut(s) 237, 247, 291, 428, 433, 700
LmnI GCTCC 1 cut(s) 731
LpnPI CCDG 6 cut(s) 87, 247, 380, 450, 539, 648
LweI GCATC 2 cut(s) 92, 519
MaeI CTAG 2 cut(s) 209, 219
MaeII ACGT 1 cut(s) 300
MalI GATC 6 cut(s) 239, 249, 293, 430, 435, 702
MbiI CCGCTC 1 cut(s) 256
MboI GATC 6 cut(s) 237, 247, 291, 428, 433, 700
MboII GAAGA 4 cut(s) 16, 360, 380, 588
MflI RGATCY 1 cut(s) 433
MluCI AATT 4 cut(s) 181, 279, 618, 652
MlyI GAGTC 3 cut(s) 34, 647, 665
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 9 cut(s) 67, 183, 205, 340, 413, 463, 652, 687, 703
MroI TCCGGA 1 cut(s) 436
MseI TTAA 2 cut(s) 342, 615
MspI CCGG 3 cut(s) 234, 437, 635
MspR9I CCNGG 2 cut(s) 235, 635
MvnI CGCG 2 cut(s) 465, 564
MwoI GCNNNNNNNGC 2 cut(s) 107, 456
NciI CCSGG 2 cut(s) 235, 635
NdeII GATC 6 cut(s) 237, 247, 291, 428, 433, 700
NlaIII CATG 3 cut(s) 485, 539, 683
NlaIV GGNNCC 2 cut(s) 216, 451
NspI RCATGY 1 cut(s) 539
PaeR7I CTCGAG 1 cut(s) 251
PaqCI CACCTGC 1 cut(s) 544
PciI ACATGT 1 cut(s) 535
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PfeI GAWTC 1 cut(s) 548
PflMI CCANNNNNTGG 1 cut(s) 136
PfoI TCCNGGA 2 cut(s) 233, 633
Ple19I CGATCG 1 cut(s) 294
PleI GAGTC 3 cut(s) 34, 646, 665
PpsI GAGTC 3 cut(s) 34, 646, 665
PpuMI RGGWCCY 1 cut(s) 215
PscI ACATGT 1 cut(s) 535
Psp5II RGGWCCY 1 cut(s) 215
PspN4I GGNNCC 2 cut(s) 216, 451
PspPI GGNCC 5 cut(s) 127, 215, 231, 449, 559
PspPPI RGGWCCY 1 cut(s) 215
PsuI RGATCY 1 cut(s) 433
PvuI CGATCG 1 cut(s) 294
SaqAI TTAA 2 cut(s) 342, 615
Sau3AI GATC 6 cut(s) 237, 247, 291, 428, 433, 700
Sau96I GGNCC 5 cut(s) 127, 215, 231, 449, 559
SchI GAGTC 3 cut(s) 34, 647, 665
ScrFI CCNGG 2 cut(s) 235, 635
SetI ASST 9 cut(s) 54, 106, 175, 220, 303, 369, 558, 714, 736
SfaNI GCATC 2 cut(s) 92, 519
Sfr274I CTCGAG 1 cut(s) 251
SinI GGWCC 3 cut(s) 215, 231, 559
SlaI CTCGAG 1 cut(s) 251
SmlI CTYRAG 3 cut(s) 251, 454, 574
SmoI CTYRAG 3 cut(s) 251, 454, 574
Sse9I AATT 4 cut(s) 181, 279, 618, 652
SsiI CCGC 2 cut(s) 256, 562
SspMI CTAG 2 cut(s) 209, 219
StyD4I CCNGG 2 cut(s) 233, 633
TaiI ACGT 1 cut(s) 303
TaqI TCGA 6 cut(s) 240, 252, 294, 346, 372, 410
TasI AATT 4 cut(s) 181, 279, 618, 652
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 2 cut(s) 342, 615
Tru9I TTAA 2 cut(s) 342, 615
TscAI CASTG 1 cut(s) 199
TspDTI ATGAA 3 cut(s) 17, 429, 470
TspGWI ACGGA 1 cut(s) 328
TspRI CASTG 1 cut(s) 199
Van91I CCANNNNNTGG 1 cut(s) 136
VpaK11BI GGWCC 3 cut(s) 215, 231, 559
XapI RAATTY 2 cut(s) 181, 279
XbaI TCTAGA 1 cut(s) 208
XceI RCATGY 1 cut(s) 539
XhoI CTCGAG 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 540
XspI CTAG 2 cut(s) 209, 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.