FvH4_6g42281

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001936
Physical Location & Seq
Reverse (-)
33016640 .. 33019885
3246 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g42281.t1

Sequence Viewer

Length: 915 bp
ATGGAGAGAATGGGAACAAGAGAGAAGAAGAAGGTTGCTGAGTGGGACAGGCCGATCTACCTACACGTGAAGGCCGGAGCCAAGTCATGTTTATGGTTCATAATTGAGGACGGACGGCGGCCAAGGGAACAAGGATCTGAAGAGAATGACGACGATGATGATAAAGTAATCTTCCGTGGATTAGATCTTCCCCAGCTTGGTTACCTGCGCCTTCCTTACAGCCTTGTGGTGTTGAACTCCCGATTATACATGATTGGTGGACATAAATATACTGGTAAAAGTTCAATAGAATTTTCTATCGGGTATGATCACTTGGACTTATGGGAACAACCAAGCAAGCAACAATGGCGTTTCGAAAAAGATGAATGGACTTTATATTCGACCGGCTCGGTGGCCTGCCCCGGTGACGGTCTCGTCTATACCTTGGCACCTCGACGCTGCTACCGTTTCCGTTTCGATCCGCGCAGGGGCGGTGATGTCCGTTCAGAGTTCCCTTCTGAATTCCCAATCAAATTAGGAGTTGCACCACATCTTGTCAGTATTTCTAATAAGTATATTTACGCCTATAGCAAAGGCGCCGGATCTAATACCTTGGTTCGGTTTGATTCGGAGAAAAAGGAGTGGGAGTGCCTTTATAGGAATTTCTGGGGTCGCTTGGCCCCGGAGTGGTGCTCTACGTGGCTCGATCAACCTGTCAAGGGACTTGATGATGCTCTTCCGGTTATCCCAGAAGGGTACGAGGAGGACCCCGTTTACAACTACTTTTTACCGCTCTTGTTCCATATTGGAACCGAAAGGTTTGCTTTGGTTTGGGACAAGTATTTGCAAAATGTCGGTTGCGAGCTTCATTGCCACAAATTCATATTCGACTACACTCGCAGCAGCGGCAGCGATAGCACTACCGAAAAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

305

Amino Acids

35.45

Weight (kDa)

6.03

Isoelectric Point (pI)

53.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 413
Acc36I ACCTGC 1 cut(s) 213
AccB1I GGYRCC 2 cut(s) 427, 575
AccBSI CCGCTC 1 cut(s) 772
AccII CGCG 1 cut(s) 463
AciI CCGC 5 cut(s) 118, 461, 471, 770, 885
AclWI GGATC 3 cut(s) 142, 452, 589
AcoI YGGCCR 1 cut(s) 119
AcsI RAATTY 4 cut(s) 290, 500, 640, 857
AcuI CTGAAG 1 cut(s) 159
AcvI CACGTG 1 cut(s) 67
AcyI GRCGYC 1 cut(s) 576
AfaI GTAC 1 cut(s) 737
AfiI CCNNNNNNNGG 6 cut(s) 197, 407, 467, 597, 666, 698
AflIII ACRYGT 1 cut(s) 64
AgsI TTSAA 2 cut(s) 235, 285
AluBI AGCT 2 cut(s) 196, 844
AluI AGCT 2 cut(s) 196, 844
Alw21I GWGCWC 1 cut(s) 674
Alw26I GTCTC 1 cut(s) 416
AlwI GGATC 3 cut(s) 142, 452, 589
AoxI GGCC 5 cut(s) 50, 72, 119, 393, 657
ApeKI GCWGC 4 cut(s) 438, 879, 882, 888
ApoI RAATTY 4 cut(s) 290, 500, 640, 857
ArsI GACNNNNNNTTYG 2 cut(s) 361, 393
AspLEI GCGC 3 cut(s) 210, 465, 578
AspS9I GGNCC 2 cut(s) 658, 745
AsuC2I CCSGG 2 cut(s) 402, 662
AsuHPI GGTGA 2 cut(s) 416, 485
AsuII TTCGAA 1 cut(s) 354
AvaII GGWCC 1 cut(s) 745
BanI GGYRCC 2 cut(s) 427, 575
BbrPI CACGTG 1 cut(s) 67
Bbv12I GWGCWC 1 cut(s) 674
BbvI GCAGC 4 cut(s) 425, 891, 894, 900
BceAI ACGGC 1 cut(s) 131
BcgI CGANNNNNNTGC 2 cut(s) 782, 816
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 2 cut(s) 402, 662
BcoDI GTCTC 1 cut(s) 416
BfmI CTRYAG 1 cut(s) 565
BfoI RGCGCY 1 cut(s) 579
BfuAI ACCTGC 1 cut(s) 213
BglII AGATCT 1 cut(s) 184
BisI GCNGC 6 cut(s) 119, 439, 880, 883, 886, 889
BlsI GCNGC 6 cut(s) 120, 440, 881, 884, 887, 890
Bme1390I CCNGG 2 cut(s) 402, 662
Bme18I GGWCC 1 cut(s) 745
BmgT120I GGNCC 2 cut(s) 658, 745
BmiI GGNNCC 6 cut(s) 79, 429, 577, 660, 747, 790
BmrFI CCNGG 2 cut(s) 402, 662
BmsI GCATC 1 cut(s) 700
BplI GAGNNNNNCTC 2 cut(s) 656, 688
Bpu14I TTCGAA 1 cut(s) 354
BpuMI CCSGG 2 cut(s) 402, 662
BsaAI YACGTR 2 cut(s) 67, 678
BsaHI GRCGYC 1 cut(s) 576
BsaI GGTCTC 1 cut(s) 416
BsaJI CCNNGG 6 cut(s) 122, 175, 400, 423, 591, 660
BsaWI WCCGGW 1 cut(s) 718
BsaXI ACNNNNNCTCC 2 cut(s) 611, 641
Bsc4I CCNNNNNNNGG 6 cut(s) 197, 407, 467, 597, 666, 698
Bse118I RCCGGY 1 cut(s) 383
Bse1I ACTGG 1 cut(s) 277
Bse3DI GCAATG 1 cut(s) 847
BseDI CCNNGG 6 cut(s) 122, 175, 400, 423, 591, 660
BseLI CCNNNNNNNGG 6 cut(s) 197, 407, 467, 597, 666, 698
BseMI GCAATG 1 cut(s) 847
BseMII CTCAG 1 cut(s) 30
BseNI ACTGG 1 cut(s) 277
BseRI GAGGAG 1 cut(s) 755
BseXI GCAGC 4 cut(s) 425, 891, 894, 900
BseYI CCCAGC 1 cut(s) 192
Bsh1236I CGCG 1 cut(s) 463
Bsh1285I CGRYCG 1 cut(s) 384
BshFI GGCC 5 cut(s) 52, 74, 121, 395, 659
BshNI GGYRCC 2 cut(s) 427, 575
BsiEI CGRYCG 1 cut(s) 384
BsiHKAI GWGCWC 1 cut(s) 674
BsiSI CCGG 6 cut(s) 75, 384, 402, 579, 662, 719
BslFI GGGAC 3 cut(s) 59, 714, 827
BslI CCNNNNNNNGG 6 cut(s) 197, 407, 467, 597, 666, 698
BsmAI GTCTC 1 cut(s) 416
BsmFI GGGAC 3 cut(s) 59, 714, 827
BsnI GGCC 5 cut(s) 52, 74, 121, 395, 659
Bso31I GGTCTC 1 cut(s) 416
Bsp119I TTCGAA 1 cut(s) 354
Bsp1286I GDGCHC 1 cut(s) 674
Bsp143I GATC 7 cut(s) 54, 134, 184, 307, 457, 581, 685
BspACI CCGC 5 cut(s) 118, 461, 471, 770, 885
BspANI GGCC 5 cut(s) 52, 74, 121, 395, 659
BspCNI CTCAG 1 cut(s) 31
BspFNI CGCG 1 cut(s) 463
BspLI GGNNCC 6 cut(s) 79, 429, 577, 660, 747, 790
BspMI ACCTGC 1 cut(s) 213
BspPI GGATC 3 cut(s) 142, 452, 589
BspQI GCTCTTC 1 cut(s) 720
BspT104I TTCGAA 1 cut(s) 354
BspT107I GGYRCC 2 cut(s) 427, 575
BspTNI GGTCTC 1 cut(s) 416
BsrBI CCGCTC 1 cut(s) 772
BsrDI GCAATG 1 cut(s) 847
BsrFI RCCGGY 1 cut(s) 383
BsrI ACTGG 1 cut(s) 277
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 6 cut(s) 122, 175, 400, 423, 591, 660
BssMI GATC 7 cut(s) 54, 134, 184, 307, 457, 581, 685
BssNI GRCGYC 1 cut(s) 576
BssT1I CCWWGG 3 cut(s) 122, 423, 591
Bst4CI ACNGT 2 cut(s) 410, 446
Bst6I CTCTTC 2 cut(s) 135, 720
BstACI GRCGYC 1 cut(s) 576
BstBAI YACGTR 2 cut(s) 67, 678
BstBI TTCGAA 1 cut(s) 354
BstC8I GCNNGC 3 cut(s) 338, 397, 842
BstDEI CTNAG 1 cut(s) 39
BstDSI CCRYGG 1 cut(s) 175
BstEII GGTNACC 1 cut(s) 200
BstFNI CGCG 1 cut(s) 463
BstH2I RGCGCY 1 cut(s) 579
BstHHI GCGC 3 cut(s) 210, 465, 578
BstKTI GATC 7 cut(s) 57, 137, 187, 310, 460, 584, 688
BstMAI GTCTC 1 cut(s) 416
BstMBI GATC 7 cut(s) 54, 134, 184, 307, 457, 581, 685
BstMCI CGRYCG 1 cut(s) 384
BstMWI GCNNNNNNNGC 4 cut(s) 346, 885, 888, 894
BstPI GGTNACC 1 cut(s) 200
BstSCI CCNGG 2 cut(s) 400, 660
BstSFI CTRYAG 1 cut(s) 565
BstUI CGCG 1 cut(s) 463
BstV1I GCAGC 4 cut(s) 425, 891, 894, 900
BstX2I RGATCY 3 cut(s) 134, 184, 581
BstYI RGATCY 3 cut(s) 134, 184, 581
BsuRI GGCC 5 cut(s) 52, 74, 121, 395, 659
BtgI CCRYGG 1 cut(s) 175
BveI ACCTGC 1 cut(s) 213
Cac8I GCNNGC 3 cut(s) 338, 397, 842
CfoI GCGC 3 cut(s) 210, 465, 578
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 2 cut(s) 658, 745
CseI GACGC 1 cut(s) 444
Csp6I GTAC 1 cut(s) 736
CviAII CATG 2 cut(s) 87, 250
CviQI GTAC 1 cut(s) 736
DdeI CTNAG 1 cut(s) 39
DinI GGCGCC 1 cut(s) 577
DpnI GATC 7 cut(s) 56, 136, 186, 309, 459, 583, 687
DpnII GATC 7 cut(s) 54, 134, 184, 307, 457, 581, 685
DrdI GACNNNNNNGTC 1 cut(s) 413
DseDI GACNNNNNNGTC 1 cut(s) 413
EaeI YGGCCR 1 cut(s) 119
Eam1104I CTCTTC 2 cut(s) 135, 720
EarI CTCTTC 2 cut(s) 135, 720
Eco130I CCWWGG 3 cut(s) 122, 423, 591
Eco31I GGTCTC 1 cut(s) 416
Eco47I GGWCC 1 cut(s) 745
Eco57I CTGAAG 1 cut(s) 159
Eco72I CACGTG 1 cut(s) 67
Eco91I GGTNACC 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 745
EcoO65I GGTNACC 1 cut(s) 200
EcoRI GAATTC 1 cut(s) 500
EcoT14I CCWWGG 3 cut(s) 122, 423, 591
EgeI GGCGCC 1 cut(s) 577
EheI GGCGCC 1 cut(s) 577
ErhI CCWWGG 3 cut(s) 122, 423, 591
FaeI CATG 2 cut(s) 90, 253
FalI AAGNNNNNCTT 2 cut(s) 787, 819
FaqI GGGAC 3 cut(s) 59, 714, 827
FatI CATG 2 cut(s) 86, 249
FbaI TGATCA 1 cut(s) 307
Fnu4HI GCNGC 6 cut(s) 119, 439, 880, 883, 886, 889
Fsp4HI GCNGC 6 cut(s) 119, 439, 880, 883, 886, 889
GlaI GCGC 3 cut(s) 209, 464, 577
GluI GCNGC 6 cut(s) 119, 439, 880, 883, 886, 889
GsaI CCCAGC 1 cut(s) 196
HaeII RGCGCY 1 cut(s) 579
HaeIII GGCC 5 cut(s) 52, 74, 121, 395, 659
HapII CCGG 6 cut(s) 75, 384, 402, 579, 662, 719
HgaI GACGC 1 cut(s) 444
HhaI GCGC 3 cut(s) 210, 465, 578
Hin1I GRCGYC 1 cut(s) 576
Hin1II CATG 2 cut(s) 90, 253
Hin6I GCGC 3 cut(s) 208, 463, 576
HinP1I GCGC 3 cut(s) 208, 463, 576
HinfI GANTC 1 cut(s) 605
HpaII CCGG 6 cut(s) 75, 384, 402, 579, 662, 719
HphI GGTGA 2 cut(s) 416, 485
Hpy166II GTNNAC 2 cut(s) 260, 754
Hpy188I TCNGA 4 cut(s) 139, 487, 499, 610
Hpy188III TCNNGA 1 cut(s) 240
Hpy8I GTNNAC 2 cut(s) 260, 754
Hpy99I CGWCG 2 cut(s) 155, 438
HpyAV CCTTC 5 cut(s) 25, 64, 221, 504, 725
HpyCH4III ACNGT 2 cut(s) 410, 446
HpyCH4IV ACGT 2 cut(s) 66, 677
HpyCH4V TGCA 2 cut(s) 524, 826
HpyF10VI GCNNNNNNNGC 4 cut(s) 346, 885, 888, 894
HpyF3I CTNAG 1 cut(s) 39
HpySE526I ACGT 2 cut(s) 66, 677
Hsp92I GRCGYC 1 cut(s) 576
Hsp92II CATG 2 cut(s) 90, 253
HspAI GCGC 3 cut(s) 208, 463, 576
KasI GGCGCC 1 cut(s) 575
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 7 cut(s) 54, 134, 184, 307, 457, 581, 685
LguI GCTCTTC 1 cut(s) 720
LmnI GCTCC 1 cut(s) 77
Lsp1109I GCAGC 4 cut(s) 425, 891, 894, 900
LweI GCATC 1 cut(s) 700
MaeII ACGT 2 cut(s) 66, 677
MaeIII GTNAC 2 cut(s) 200, 404
MalI GATC 7 cut(s) 56, 136, 186, 309, 459, 583, 687
MbiI CCGCTC 1 cut(s) 772
MboI GATC 7 cut(s) 54, 134, 184, 307, 457, 581, 685
MboII GAAGA 6 cut(s) 37, 40, 152, 163, 179, 707
MflI RGATCY 3 cut(s) 134, 184, 581
MhlI GDGCHC 1 cut(s) 674
MluCI AATT 7 cut(s) 102, 290, 500, 512, 640, 857, 910
Mly113I GGCGCC 1 cut(s) 576
MnlI CCTC 4 cut(s) 100, 441, 733, 736
MseI TTAA 1 cut(s) 913
MslI CAYNNNNRTG 1 cut(s) 91
MspA1I CMGCKG 1 cut(s) 885
MspI CCGG 6 cut(s) 75, 384, 402, 579, 662, 719
MspR9I CCNGG 2 cut(s) 402, 662
MvnI CGCG 1 cut(s) 463
MwoI GCNNNNNNNGC 4 cut(s) 346, 885, 888, 894
NarI GGCGCC 1 cut(s) 576
NciI CCSGG 2 cut(s) 402, 662
NdeII GATC 7 cut(s) 54, 134, 184, 307, 457, 581, 685
NlaIII CATG 2 cut(s) 90, 253
NlaIV GGNNCC 6 cut(s) 79, 429, 577, 660, 747, 790
NmuCI GTSAC 1 cut(s) 404
NspV TTCGAA 1 cut(s) 354
PciSI GCTCTTC 1 cut(s) 720
PcsI WCGNNNNNNNCGW 1 cut(s) 442
PfeI GAWTC 1 cut(s) 605
PkrI GCNGC 6 cut(s) 120, 440, 881, 884, 887, 890
PluTI GGCGCC 1 cut(s) 579
PmaCI CACGTG 1 cut(s) 67
PmlI CACGTG 1 cut(s) 67
Ppu21I YACGTR 2 cut(s) 67, 678
PpuMI RGGWCCY 1 cut(s) 745
Psp5II RGGWCCY 1 cut(s) 745
PspCI CACGTG 1 cut(s) 67
PspEI GGTNACC 1 cut(s) 200
PspFI CCCAGC 1 cut(s) 192
PspN4I GGNNCC 6 cut(s) 79, 429, 577, 660, 747, 790
PspPI GGNCC 2 cut(s) 658, 745
PspPPI RGGWCCY 1 cut(s) 745
PsuI RGATCY 3 cut(s) 134, 184, 581
RsaI GTAC 1 cut(s) 737
RsaNI GTAC 1 cut(s) 736
RseI CAYNNNNRTG 1 cut(s) 91
SapI GCTCTTC 1 cut(s) 720
SaqAI TTAA 1 cut(s) 913
SatI GCNGC 6 cut(s) 119, 439, 880, 883, 886, 889
Sau3AI GATC 7 cut(s) 54, 134, 184, 307, 457, 581, 685
Sau96I GGNCC 2 cut(s) 658, 745
ScrFI CCNGG 2 cut(s) 402, 662
SduI GDGCHC 1 cut(s) 674
SfaNI GCATC 1 cut(s) 700
SfcI CTRYAG 1 cut(s) 565
SfoI GGCGCC 1 cut(s) 577
SfuI TTCGAA 1 cut(s) 354
SinI GGWCC 1 cut(s) 745
SmiMI CAYNNNNRTG 1 cut(s) 91
Sse9I AATT 7 cut(s) 102, 290, 500, 512, 640, 857, 910
SsiI CCGC 5 cut(s) 118, 461, 471, 770, 885
SspDI GGCGCC 1 cut(s) 575
StyD4I CCNGG 2 cut(s) 400, 660
StyI CCWWGG 3 cut(s) 122, 423, 591
TaaI ACNGT 2 cut(s) 410, 446
TaiI ACGT 2 cut(s) 69, 680
TaqI TCGA 6 cut(s) 354, 380, 433, 456, 684, 867
TasI AATT 7 cut(s) 102, 290, 500, 512, 640, 857, 910
TauI GCSGC 2 cut(s) 121, 888
TfiI GAWTC 1 cut(s) 605
Tru1I TTAA 1 cut(s) 913
Tru9I TTAA 1 cut(s) 913
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 4 cut(s) 438, 879, 882, 888
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 4 cut(s) 88, 378, 836, 850
TspGWI ACGGA 4 cut(s) 126, 164, 440, 470
VpaK11BI GGWCC 1 cut(s) 745
XapI RAATTY 4 cut(s) 290, 500, 640, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.