Rh1DG349400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001936
Physical Location & Seq
Reverse (-)
57429211 .. 57429798
588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG349400.1

Sequence Viewer

Length: 588 bp
ATGGGAAAGAAAAACAAAACGAGGGACAAGAAGAAGAAGGTTGCTTGGGATAGGCCCATCTACCTGTACCTACAAGTCAAGGAAGAGGGCAACTCGGTATCGTCTTTATGGTTCGTCATTGAAGATGGACGGCGGCCAAGGCAAATCAGTGGATCTGAAGAGATTGATGATGATGATGATAAGGTAATCTTCCGTCCCCTAGATCTTCCCGAATTTGATGATCATTACCTGCGCCTTCCTTACAGCGTTGTGGTCTTGGACTCCCGATTATTCATGATTAGTGGATATCAATGTAGAAAGCGAGATTCCTCTGGTGTGAAACCCTATATTGGGTATGATTACTTGGACCTGAAACCCTCCTCTGTAGGCCAGGAATGGCGTTCTGTACAAGGGGTTTCATTCCCTTCGCAAGAGTCATTGGCCTGCTGCAAAGACGGTTTCATCTATACCTTGGGACAGCAATGTTGTCACCGTCTTGATCCTCGCTCTGGTGTCTGTTCGGAGTTTCCCGCCCGCCACATGCGGGTTTCGAACACAGCTTGGTATATCGAACAAGTACATTTACACCTATTCCCTTGGTATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

195

Amino Acids

22.8

Weight (kDa)

7.6

Isoelectric Point (pI)

59.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 586
Acc36I ACCTGC 1 cut(s) 237
AciI CCGC 4 cut(s) 133, 510, 514, 523
AclWI GGATC 2 cut(s) 160, 473
AcoI YGGCCR 1 cut(s) 134
AcsI RAATTY 1 cut(s) 212
AcuI CTGAAG 1 cut(s) 177
AfaI GTAC 3 cut(s) 68, 387, 558
AfiI CCNNNNNNNGG 4 cut(s) 329, 330, 488, 523
AgsI TTSAA 1 cut(s) 122
AjnI CCWGG 1 cut(s) 369
AluBI AGCT 1 cut(s) 539
AluI AGCT 1 cut(s) 539
AlwI GGATC 2 cut(s) 160, 473
AoxI GGCC 4 cut(s) 53, 134, 367, 420
ApeKI GCWGC 1 cut(s) 426
ApoI RAATTY 1 cut(s) 212
AspLEI GCGC 1 cut(s) 234
AspS9I GGNCC 2 cut(s) 54, 346
AsuHPI GGTGA 1 cut(s) 461
AsuII TTCGAA 1 cut(s) 530
AvaII GGWCC 1 cut(s) 346
BbvI GCAGC 1 cut(s) 413
BccI CCATC 2 cut(s) 65, 119
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 371
BclI TGATCA 1 cut(s) 220
BfaI CTAG 1 cut(s) 200
BfmI CTRYAG 1 cut(s) 363
BfuAI ACCTGC 1 cut(s) 237
BglII AGATCT 1 cut(s) 202
BisI GCNGC 2 cut(s) 134, 427
BlsI GCNGC 2 cut(s) 135, 428
Bme1390I CCNGG 1 cut(s) 371
Bme18I GGWCC 1 cut(s) 346
BmgT120I GGNCC 2 cut(s) 54, 346
BmrFI CCNGG 1 cut(s) 371
BplI GAGNNNNNCTC 2 cut(s) 77, 109
Bpu14I TTCGAA 1 cut(s) 530
BsaJI CCNNGG 3 cut(s) 137, 450, 575
Bsc4I CCNNNNNNNGG 4 cut(s) 329, 330, 488, 523
Bse3DI GCAATG 1 cut(s) 467
BseBI CCWGG 1 cut(s) 371
BseDI CCNNGG 3 cut(s) 137, 450, 575
BseLI CCNNNNNNNGG 4 cut(s) 329, 330, 488, 523
BseMI GCAATG 1 cut(s) 467
BseRI GAGGAG 1 cut(s) 349
BseXI GCAGC 1 cut(s) 413
BshFI GGCC 4 cut(s) 55, 136, 369, 422
BslFI GGGAC 3 cut(s) 38, 180, 468
BslI CCNNNNNNNGG 4 cut(s) 329, 330, 488, 523
BsmFI GGGAC 3 cut(s) 38, 180, 468
BsnI GGCC 4 cut(s) 55, 136, 369, 422
Bsp119I TTCGAA 1 cut(s) 530
Bsp1407I TGTACA 1 cut(s) 385
Bsp143I GATC 4 cut(s) 152, 202, 220, 478
BspACI CCGC 4 cut(s) 133, 510, 514, 523
BspANI GGCC 4 cut(s) 55, 136, 369, 422
BspHI TCATGA 1 cut(s) 273
BspMI ACCTGC 1 cut(s) 237
BspPI GGATC 2 cut(s) 160, 473
BspT104I TTCGAA 1 cut(s) 530
BsrDI GCAATG 1 cut(s) 467
BsrGI TGTACA 1 cut(s) 385
BssECI CCNNGG 3 cut(s) 137, 450, 575
BssMI GATC 4 cut(s) 152, 202, 220, 478
BssT1I CCWWGG 3 cut(s) 137, 450, 575
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 2 cut(s) 437, 473
Bst6I CTCTTC 2 cut(s) 78, 153
BstAUI TGTACA 1 cut(s) 385
BstBI TTCGAA 1 cut(s) 530
BstC8I GCNNGC 2 cut(s) 424, 514
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 4 cut(s) 155, 205, 223, 481
BstMBI GATC 4 cut(s) 152, 202, 220, 478
BstMWI GCNNNNNNNGC 1 cut(s) 139
BstNI CCWGG 1 cut(s) 371
BstNSI RCATGY 1 cut(s) 523
BstSCI CCNGG 1 cut(s) 369
BstSFI CTRYAG 1 cut(s) 363
BstV1I GCAGC 1 cut(s) 413
BstX2I RGATCY 2 cut(s) 152, 202
BstYI RGATCY 2 cut(s) 152, 202
BsuRI GGCC 4 cut(s) 55, 136, 369, 422
BtsIMutI CAGTG 1 cut(s) 154
BveI ACCTGC 1 cut(s) 237
Cac8I GCNNGC 2 cut(s) 424, 514
CciI TCATGA 1 cut(s) 273
CfoI GCGC 1 cut(s) 234
Cfr13I GGNCC 2 cut(s) 54, 346
Csp6I GTAC 3 cut(s) 67, 386, 557
CviAII CATG 2 cut(s) 274, 520
CviJI RGCY 5 cut(s) 55, 136, 369, 422, 539
CviKI_1 RGCY 5 cut(s) 55, 136, 369, 422, 539
CviQI GTAC 3 cut(s) 67, 386, 557
DpnI GATC 4 cut(s) 154, 204, 222, 480
DpnII GATC 4 cut(s) 152, 202, 220, 478
EaeI YGGCCR 1 cut(s) 134
Eam1104I CTCTTC 2 cut(s) 78, 153
EarI CTCTTC 2 cut(s) 78, 153
Eco130I CCWWGG 3 cut(s) 137, 450, 575
Eco32I GATATC 1 cut(s) 287
Eco47I GGWCC 1 cut(s) 346
Eco57I CTGAAG 1 cut(s) 177
EcoRII CCWGG 1 cut(s) 369
EcoRV GATATC 1 cut(s) 287
EcoT14I CCWWGG 3 cut(s) 137, 450, 575
ErhI CCWWGG 3 cut(s) 137, 450, 575
FaeI CATG 2 cut(s) 277, 523
FaiI YATR 8 cut(s) 109, 275, 327, 336, 447, 521, 546, 586
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FaqI GGGAC 3 cut(s) 38, 180, 468
FatI CATG 2 cut(s) 273, 519
FauI CCCGC 3 cut(s) 516, 517, 521
FbaI TGATCA 1 cut(s) 220
Fnu4HI GCNGC 2 cut(s) 134, 427
Fsp4HI GCNGC 2 cut(s) 134, 427
FspBI CTAG 1 cut(s) 200
GlaI GCGC 1 cut(s) 233
GluI GCNGC 2 cut(s) 134, 427
HaeIII GGCC 4 cut(s) 55, 136, 369, 422
HhaI GCGC 1 cut(s) 234
Hin1II CATG 2 cut(s) 277, 523
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HinfI GANTC 3 cut(s) 260, 305, 413
HphI GGTGA 1 cut(s) 461
Hpy188I TCNGA 2 cut(s) 157, 502
Hpy188III TCNNGA 4 cut(s) 209, 264, 274, 476
HpyAV CCTTC 3 cut(s) 31, 245, 414
HpyCH4III ACNGT 2 cut(s) 437, 473
HpyCH4V TGCA 1 cut(s) 429
HpyF10VI GCNNNNNNNGC 1 cut(s) 139
Hsp92II CATG 2 cut(s) 277, 523
HspAI GCGC 1 cut(s) 232
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 4 cut(s) 152, 202, 220, 478
LpnPI CCDG 8 cut(s) 77, 242, 297, 356, 362, 383, 436, 474
Lsp1109I GCAGC 1 cut(s) 413
MaeI CTAG 1 cut(s) 200
MaeIII GTNAC 1 cut(s) 467
MalI GATC 4 cut(s) 154, 204, 222, 480
MboI GATC 4 cut(s) 152, 202, 220, 478
MboII GAAGA 7 cut(s) 43, 46, 95, 134, 170, 181, 197
MflI RGATCY 2 cut(s) 152, 202
MluCI AATT 1 cut(s) 212
MlyI GAGTC 2 cut(s) 254, 422
MnlI CCTC 6 cut(s) 15, 79, 319, 367, 370, 492
MspR9I CCNGG 1 cut(s) 371
MvaI CCWGG 1 cut(s) 371
MwoI GCNNNNNNNGC 1 cut(s) 139
NdeII GATC 4 cut(s) 152, 202, 220, 478
NlaIII CATG 2 cut(s) 277, 523
NmuCI GTSAC 1 cut(s) 467
NspI RCATGY 1 cut(s) 523
NspV TTCGAA 1 cut(s) 530
PagI TCATGA 1 cut(s) 273
PfeI GAWTC 1 cut(s) 305
PkrI GCNGC 2 cut(s) 135, 428
PleI GAGTC 2 cut(s) 254, 421
PpsI GAGTC 2 cut(s) 254, 421
PsiI TTATAA 1 cut(s) 586
Psp6I CCWGG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 369
PspPI GGNCC 2 cut(s) 54, 346
PsuI RGATCY 2 cut(s) 152, 202
RsaI GTAC 3 cut(s) 68, 387, 558
RsaNI GTAC 3 cut(s) 67, 386, 557
SatI GCNGC 2 cut(s) 134, 427
Sau3AI GATC 4 cut(s) 152, 202, 220, 478
Sau96I GGNCC 2 cut(s) 54, 346
SchI GAGTC 2 cut(s) 254, 422
ScrFI CCNGG 1 cut(s) 371
SetI ASST 9 cut(s) 42, 66, 72, 186, 231, 351, 452, 541, 570
SfcI CTRYAG 1 cut(s) 363
SfuI TTCGAA 1 cut(s) 530
SinI GGWCC 1 cut(s) 346
Sse9I AATT 1 cut(s) 212
SsiI CCGC 4 cut(s) 133, 510, 514, 523
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 369
StyI CCWWGG 3 cut(s) 137, 450, 575
TaaI ACNGT 2 cut(s) 437, 473
TaqI TCGA 2 cut(s) 530, 549
TasI AATT 1 cut(s) 212
TatI WGTACW 2 cut(s) 385, 556
TauI GCSGC 1 cut(s) 136
TfiI GAWTC 1 cut(s) 305
TscAI CASTG 1 cut(s) 154
TseFI GTSAC 1 cut(s) 467
TseI GCWGC 1 cut(s) 426
Tsp45I GTSAC 1 cut(s) 467
TspDTI ATGAA 3 cut(s) 262, 387, 430
TspGWI ACGGA 1 cut(s) 182
TspRI CASTG 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 346
XapI RAATTY 1 cut(s) 212
XceI RCATGY 1 cut(s) 523
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.