FvH4_7g32080

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003280
Physical Location & Seq
Forward (+)
23108486 .. 23109225
740 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g32080.t1

Sequence Viewer

Length: 456 bp
ATGGGTTCTCATCTCCAAAGCTTCTGCATGTCTACAATGATATTGCTCATTGTTTTCTCAGCAACCCTAGCAGTATGTCAGCCCCCTTCCGCTAGTTCCTTGGAACAAAACAACCCAGCTAATATCTCCCAGATTTTTGAAGATTGGATGGCAAAGCACAATCGTGTCTACTCCACCAGTGAAGAGAAAACAAGACGGTTTGAGATCTTCAAAGCCAAATATCTCTATGTGCAAAAGTTCAACAATGAAGGGGGACACACGTGGACTGCTGGGCTTAATGCCTTTTCAGACCAGACGACAGCGGAGTTCAGTTCTCGGTGCTGCGGCCGCCGCCCATCTCGCCTTAGGCGTAGGCGATATGATCTCAAGCCTACAGCCCCAATGTACCGGAGATACCGTTTCATGCCAAGGAGAGCCCTGGGATTTGAAGATCAGGTCTTGGAACCTGGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

152

Amino Acids

17.54

Weight (kDa)

9.78

Isoelectric Point (pI)

76.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 46 - 103 2.8e-17 Cathepsin propeptide inhibitor domain (I29)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 32, 168
AciI CCGC 5 cut(s) 90, 302, 324, 328, 331
AcoI YGGCCR 1 cut(s) 325
AcvI CACGTG 1 cut(s) 261
AfaI GTAC 1 cut(s) 386
AflIII ACRYGT 1 cut(s) 258
AgsI TTSAA 4 cut(s) 140, 211, 241, 428
AjnI CCWGG 2 cut(s) 417, 445
AleI CACNNNNGTG 1 cut(s) 162
AluBI AGCT 2 cut(s) 21, 119
AluI AGCT 2 cut(s) 21, 119
AoxI GGCC 1 cut(s) 325
ApeKI GCWGC 1 cut(s) 321
AxyI CCTNAGG 1 cut(s) 344
BanII GRGCYC 1 cut(s) 418
BbrPI CACGTG 1 cut(s) 261
BbvI GCAGC 1 cut(s) 308
BccI CCATC 2 cut(s) 142, 343
BciT130I CCWGG 2 cut(s) 419, 447
BfaI CTAG 2 cut(s) 68, 93
BfmI CTRYAG 1 cut(s) 372
BglII AGATCT 1 cut(s) 204
BisI GCNGC 4 cut(s) 322, 325, 328, 331
BlsI GCNGC 4 cut(s) 323, 326, 329, 332
Bme1390I CCNGG 2 cut(s) 419, 447
BmiI GGNNCC 1 cut(s) 444
BmrFI CCNGG 2 cut(s) 419, 447
BpuEI CTTGAG 1 cut(s) 350
BsaAI YACGTR 1 cut(s) 261
BsaJI CCNNGG 5 cut(s) 99, 407, 417, 418, 446
BsaWI WCCGGW 1 cut(s) 387
BsaXI ACNNNNNCTCC 2 cut(s) 382, 412
Bse1I ACTGG 1 cut(s) 177
Bse21I CCTNAGG 1 cut(s) 344
BseBI CCWGG 2 cut(s) 419, 447
BseDI CCNNGG 5 cut(s) 99, 407, 417, 418, 446
BseGI GGATG 1 cut(s) 153
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 1 cut(s) 177
BseX3I CGGCCG 1 cut(s) 325
BseXI GCAGC 1 cut(s) 308
BseYI CCCAGC 2 cut(s) 115, 269
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 1 cut(s) 327
BsiEI CGRYCG 1 cut(s) 328
BsiSI CCGG 1 cut(s) 388
BslFI GGGAC 1 cut(s) 267
BsmFI GGGAC 1 cut(s) 267
BsnI GGCC 1 cut(s) 327
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 3 cut(s) 204, 361, 430
BspACI CCGC 5 cut(s) 90, 302, 324, 328, 331
BspANI GGCC 1 cut(s) 327
BspCNI CTCAG 1 cut(s) 71
BspLI GGNNCC 1 cut(s) 444
BsrI ACTGG 1 cut(s) 177
BssECI CCNNGG 5 cut(s) 99, 407, 417, 418, 446
BssMI GATC 3 cut(s) 204, 361, 430
BssT1I CCWWGG 2 cut(s) 99, 407
Bst2UI CCWGG 2 cut(s) 419, 447
Bst4CI ACNGT 2 cut(s) 198, 398
Bst6I CTCTTC 1 cut(s) 177
BstBAI YACGTR 1 cut(s) 261
BstDEI CTNAG 2 cut(s) 58, 344
BstF5I GGATG 1 cut(s) 153
BstKTI GATC 3 cut(s) 207, 364, 433
BstMBI GATC 3 cut(s) 204, 361, 430
BstMCI CGRYCG 1 cut(s) 328
BstMWI GCNNNNNNNGC 4 cut(s) 68, 327, 330, 339
BstNI CCWGG 2 cut(s) 419, 447
BstNSI RCATGY 1 cut(s) 31
BstSCI CCNGG 2 cut(s) 417, 445
BstSFI CTRYAG 1 cut(s) 372
BstV1I GCAGC 1 cut(s) 308
BstX2I RGATCY 1 cut(s) 204
BstYI RGATCY 1 cut(s) 204
BstZI CGGCCG 1 cut(s) 325
Bsu36I CCTNAGG 1 cut(s) 344
BsuRI GGCC 1 cut(s) 327
BtsCI GGATG 1 cut(s) 153
BtsIMutI CAGTG 1 cut(s) 184
CciNI GCGGCCGC 1 cut(s) 325
Csp6I GTAC 1 cut(s) 385
CviAII CATG 2 cut(s) 28, 403
CviJI RGCY 9 cut(s) 21, 82, 119, 215, 274, 327, 370, 377, 416
CviKI_1 RGCY 9 cut(s) 21, 82, 119, 215, 274, 327, 370, 377, 416
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 2 cut(s) 58, 344
DpnI GATC 3 cut(s) 206, 363, 432
DpnII GATC 3 cut(s) 204, 361, 430
EaeI YGGCCR 1 cut(s) 325
EagI CGGCCG 1 cut(s) 325
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
EclXI CGGCCG 1 cut(s) 325
Eco130I CCWWGG 2 cut(s) 99, 407
Eco24I GRGCYC 1 cut(s) 418
Eco52I CGGCCG 1 cut(s) 325
Eco72I CACGTG 1 cut(s) 261
Eco81I CCTNAGG 1 cut(s) 344
EcoRII CCWGG 2 cut(s) 417, 445
EcoT14I CCWWGG 2 cut(s) 99, 407
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 2 cut(s) 99, 407
FaeI CATG 2 cut(s) 31, 406
FaiI YATR 5 cut(s) 29, 76, 228, 360, 404
FaqI GGGAC 1 cut(s) 267
FatI CATG 2 cut(s) 27, 402
FblI GTMKAC 2 cut(s) 32, 168
Fnu4HI GCNGC 4 cut(s) 322, 325, 328, 331
FokI GGATG 1 cut(s) 160
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 4 cut(s) 322, 325, 328, 331
FspBI CTAG 2 cut(s) 68, 93
GluI GCNGC 4 cut(s) 322, 325, 328, 331
GsaI CCCAGC 2 cut(s) 119, 273
HaeIII GGCC 1 cut(s) 327
HapII CCGG 1 cut(s) 388
Hin1II CATG 2 cut(s) 31, 406
HindIII AAGCTT 1 cut(s) 19
HpaII CCGG 1 cut(s) 388
Hpy166II GTNNAC 3 cut(s) 33, 169, 264
Hpy188I TCNGA 1 cut(s) 289
Hpy8I GTNNAC 3 cut(s) 33, 169, 264
HpyAV CCTTC 2 cut(s) 96, 242
HpyCH4III ACNGT 2 cut(s) 198, 398
HpyCH4IV ACGT 1 cut(s) 260
HpyCH4V TGCA 2 cut(s) 27, 232
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 327, 330, 339
HpyF3I CTNAG 2 cut(s) 58, 344
HpySE526I ACGT 1 cut(s) 260
Hsp92II CATG 2 cut(s) 31, 406
Kzo9I GATC 3 cut(s) 204, 361, 430
Lsp1109I GCAGC 1 cut(s) 308
MaeI CTAG 2 cut(s) 68, 93
MaeII ACGT 1 cut(s) 260
MalI GATC 3 cut(s) 206, 363, 432
MboI GATC 3 cut(s) 204, 361, 430
MboII GAAGA 4 cut(s) 152, 194, 199, 440
MflI RGATCY 1 cut(s) 204
MhlI GDGCHC 1 cut(s) 418
MseI TTAA 1 cut(s) 276
MslI CAYNNNNRTG 1 cut(s) 162
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 1 cut(s) 388
MspR9I CCNGG 2 cut(s) 419, 447
MvaI CCWGG 2 cut(s) 419, 447
MwoI GCNNNNNNNGC 4 cut(s) 68, 327, 330, 339
NdeII GATC 3 cut(s) 204, 361, 430
NlaIII CATG 2 cut(s) 31, 406
NlaIV GGNNCC 1 cut(s) 444
NotI GCGGCCGC 1 cut(s) 325
NspI RCATGY 1 cut(s) 31
OliI CACNNNNGTG 1 cut(s) 162
PasI CCCWGGG 1 cut(s) 418
PcsI WCGNNNNNNNCGW 1 cut(s) 346
PkrI GCNGC 4 cut(s) 323, 326, 329, 332
PmaCI CACGTG 1 cut(s) 261
PmlI CACGTG 1 cut(s) 261
Ppu21I YACGTR 1 cut(s) 261
Psp6I CCWGG 2 cut(s) 417, 445
PspCI CACGTG 1 cut(s) 261
PspFI CCCAGC 2 cut(s) 115, 269
PspGI CCWGG 2 cut(s) 417, 445
PspN4I GGNNCC 1 cut(s) 444
PsuI RGATCY 1 cut(s) 204
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
RseI CAYNNNNRTG 1 cut(s) 162
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 4 cut(s) 322, 325, 328, 331
Sau3AI GATC 3 cut(s) 204, 361, 430
ScrFI CCNGG 2 cut(s) 419, 447
SduI GDGCHC 1 cut(s) 418
SetI ASST 5 cut(s) 23, 121, 263, 438, 448
SfcI CTRYAG 1 cut(s) 372
SmiMI CAYNNNNRTG 1 cut(s) 162
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
SsiI CCGC 5 cut(s) 90, 302, 324, 328, 331
SspMI CTAG 2 cut(s) 68, 93
StyD4I CCNGG 2 cut(s) 417, 445
StyI CCWWGG 2 cut(s) 99, 407
TaaI ACNGT 2 cut(s) 198, 398
TaiI ACGT 1 cut(s) 263
TauI GCSGC 3 cut(s) 327, 330, 333
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 1 cut(s) 184
TseI GCWGC 1 cut(s) 321
TspDTI ATGAA 2 cut(s) 261, 391
TspRI CASTG 1 cut(s) 184
XceI RCATGY 1 cut(s) 31
XmiI GTMKAC 2 cut(s) 32, 168
XspI CTAG 2 cut(s) 68, 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.