Rmu_ssc0000154.1_g000024

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000154.1
OG0003280
Physical Location & Seq
Reverse (-)
134230 .. 135979
1750 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000154.1_g000024.1.cds

Sequence Viewer

Length: 798 bp
atggcttatacaaaaagcctcgcgatcacctccacattattattcatcatgtggctggggattttgggaacatcagaagccacatcccgcagattcaaagagaatccaaattctactcttttcctagaatttgagcgatggatggcagccttcgacaaacattacccgaacgacgaagagaaggagaggcgtttcgcaatcttcaagaacaagttggagtgggtcaagaacttcaacaagcaagggaatcactcttttacggtaggaatcaatcaattttctgatatgacccctgatgaattccggctatgtgctactggccgccgccgcagccaccccagcttaagctctagtgtggtcgactcctccaggtggctgatctgggttctgcctttatttggcattatagccgtgttgatgtgtggtacggctatggagcagggttgcccaccaagcttgctgccggcaaagcagccgcaccacatccaaacctcgccgcactgccgctccgcaaagcagacccccctagaacccaacagacgtactagactgaagtcccagccgctggccagatgccgcaatgacgccatcacccgtcaccggcaagccccaccccagagctgctccactcctgccctcatccccgatcccagaagaatagatcgagatcgatcagtcctctccaagatcccgcaaccattctccaaccctggaccaaaccgttaccacaaaacaacgctgctagcccgaatcaccatcctctcccaggccagaacgaagcggcagcaacgcaggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04210 ko04612 ko04621 ko04924 map04140 map04142 map04210 map04612 map04621 map04924
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

265

Amino Acids

30.49

Weight (kDa)

10.66

Isoelectric Point (pI)

67.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 783
Acc36I ACCTGC 1 cut(s) 783
AccB7I CCANNNNNTGG 1 cut(s) 565
AccBSI CCGCTC 1 cut(s) 507
AccI GTMKAC 1 cut(s) 360
AccII CGCG 1 cut(s) 23
AclWI GGATC 2 cut(s) 641, 682
AcoI YGGCCR 2 cut(s) 319, 567
AcsI RAATTY 3 cut(s) 109, 128, 299
AcuI CTGAAG 1 cut(s) 572
AcyI GRCGYC 1 cut(s) 585
AfaI GTAC 2 cut(s) 427, 544
AfiI CCNNNNNNNGG 5 cut(s) 372, 398, 565, 600, 766
AflII CTTAAG 1 cut(s) 343
AgsI TTSAA 3 cut(s) 97, 205, 235
AjnI CCWGG 3 cut(s) 368, 709, 765
AluBI AGCT 4 cut(s) 342, 348, 456, 621
AluI AGCT 4 cut(s) 342, 348, 456, 621
AlwI GGATC 2 cut(s) 641, 682
AlwNI CAGNNNCTG 1 cut(s) 565
AoxI GGCC 3 cut(s) 319, 567, 768
ApeKI GCWGC 7 cut(s) 146, 330, 460, 472, 621, 739, 784
ApoI RAATTY 3 cut(s) 109, 128, 299
AspS9I GGNCC 1 cut(s) 713
AsuHPI GGTGA 4 cut(s) 19, 583, 590, 745
AsuNHI GCTAGC 1 cut(s) 742
AvaII GGWCC 1 cut(s) 713
BalI TGGCCA 1 cut(s) 569
BbvI GCAGC 6 cut(s) 158, 342, 447, 484, 608, 726
BccI CCATC 4 cut(s) 132, 136, 596, 764
BceAI ACGGC 2 cut(s) 395, 444
BciT130I CCWGG 3 cut(s) 370, 711, 767
BfaI CTAG 5 cut(s) 125, 351, 527, 546, 743
BfrI CTTAAG 1 cut(s) 343
BfuAI ACCTGC 1 cut(s) 783
Bme1390I CCNGG 3 cut(s) 370, 711, 767
Bme18I GGWCC 1 cut(s) 713
BmgT120I GGNCC 1 cut(s) 713
BmrFI CCNGG 3 cut(s) 370, 711, 767
BmsI GCATC 1 cut(s) 563
BmtI GCTAGC 1 cut(s) 746
BoxI GACNNNNGTC 1 cut(s) 553
BpmI CTGGAG 1 cut(s) 352
Bsa29I ATCGAT 1 cut(s) 670
BsaBI GATNNNNATC 1 cut(s) 666
BsaHI GRCGYC 1 cut(s) 585
BsaJI CCNNGG 2 cut(s) 709, 765
BsaXI ACNNNNNCTCC 2 cut(s) 491, 521
Bsc4I CCNNNNNNNGG 5 cut(s) 372, 398, 565, 600, 766
Bse118I RCCGGY 2 cut(s) 463, 600
Bse1I ACTGG 1 cut(s) 322
Bse3DI GCAATG 1 cut(s) 586
Bse8I GATNNNNATC 1 cut(s) 666
BseBI CCWGG 3 cut(s) 370, 711, 767
BseCI ATCGAT 1 cut(s) 670
BseDI CCNNGG 2 cut(s) 709, 765
BseGI GGATG 5 cut(s) 83, 147, 483, 639, 756
BseJI GATNNNNATC 1 cut(s) 666
BseLI CCNNNNNNNGG 5 cut(s) 372, 398, 565, 600, 766
BseMI GCAATG 1 cut(s) 586
BseNI ACTGG 1 cut(s) 322
BseRI GAGGAG 1 cut(s) 355
BseXI GCAGC 6 cut(s) 158, 342, 447, 484, 608, 726
BseYI CCCAGC 3 cut(s) 55, 338, 558
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 3 cut(s) 321, 569, 770
BshVI ATCGAT 1 cut(s) 670
BsiSI CCGG 3 cut(s) 304, 464, 601
BslFI GGGAC 1 cut(s) 541
BslI CCNNNNNNNGG 5 cut(s) 372, 398, 565, 600, 766
BsmFI GGGAC 1 cut(s) 541
BsnI GGCC 3 cut(s) 321, 569, 770
Bsp143I GATC 7 cut(s) 24, 378, 646, 661, 667, 671, 687
Bsp68I TCGCGA 1 cut(s) 23
BspANI GGCC 3 cut(s) 321, 569, 770
BspDI ATCGAT 1 cut(s) 670
BspFNI CGCG 1 cut(s) 23
BspMI ACCTGC 1 cut(s) 783
BspOI GCTAGC 1 cut(s) 746
BspPI GGATC 2 cut(s) 641, 682
BspTI CTTAAG 1 cut(s) 343
BsrBI CCGCTC 1 cut(s) 507
BsrDI GCAATG 1 cut(s) 586
BsrFI RCCGGY 2 cut(s) 463, 600
BsrI ACTGG 1 cut(s) 322
BssAI RCCGGY 2 cut(s) 463, 600
BssECI CCNNGG 2 cut(s) 709, 765
BssMI GATC 7 cut(s) 24, 378, 646, 661, 667, 671, 687
BssNI GRCGYC 1 cut(s) 585
Bst2UI CCWGG 3 cut(s) 370, 711, 767
Bst4CI ACNGT 2 cut(s) 262, 722
Bst6I CTCTTC 1 cut(s) 171
BstACI GRCGYC 1 cut(s) 585
BstAFI CTTAAG 1 cut(s) 343
BstC8I GCNNGC 5 cut(s) 458, 465, 567, 606, 744
BstENI CCTNNNNNAGG 1 cut(s) 764
BstF5I GGATG 5 cut(s) 83, 147, 483, 639, 756
BstFNI CGCG 1 cut(s) 23
BstKTI GATC 7 cut(s) 27, 381, 649, 664, 670, 674, 690
BstMBI GATC 7 cut(s) 24, 378, 646, 661, 667, 671, 687
BstMWI GCNNNNNNNGC 5 cut(s) 327, 330, 339, 453, 469
BstNI CCWGG 3 cut(s) 370, 711, 767
BstPAI GACNNNNGTC 1 cut(s) 553
BstSCI CCNGG 3 cut(s) 368, 709, 765
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 6 cut(s) 158, 342, 447, 484, 608, 726
BstX2I RGATCY 1 cut(s) 687
BstYI RGATCY 1 cut(s) 687
Bsu15I ATCGAT 1 cut(s) 670
BsuRI GGCC 3 cut(s) 321, 569, 770
BsuTUI ATCGAT 1 cut(s) 670
BtgZI GCGATG 1 cut(s) 151
BtsCI GGATG 5 cut(s) 83, 147, 483, 639, 756
BtsI GCAGTG 1 cut(s) 499
BtsIMutI CAGTG 1 cut(s) 499
BtuMI TCGCGA 1 cut(s) 23
BveI ACCTGC 1 cut(s) 783
Cac8I GCNNGC 5 cut(s) 458, 465, 567, 606, 744
CaiI CAGNNNCTG 1 cut(s) 565
Cfr10I RCCGGY 2 cut(s) 463, 600
Cfr13I GGNCC 1 cut(s) 713
ClaI ATCGAT 1 cut(s) 670
CseI GACGC 1 cut(s) 593
Csp6I GTAC 2 cut(s) 426, 543
CspCI CAANNNNNGTGG 2 cut(s) 438, 473
CviAII CATG 1 cut(s) 49
CviQI GTAC 2 cut(s) 426, 543
DpnI GATC 7 cut(s) 26, 380, 648, 663, 669, 673, 689
DpnII GATC 7 cut(s) 24, 378, 646, 661, 667, 671, 687
EaeI YGGCCR 2 cut(s) 319, 567
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 713
Eco57I CTGAAG 1 cut(s) 572
EcoNI CCTNNNNNAGG 1 cut(s) 764
EcoRI GAATTC 1 cut(s) 299
EcoRII CCWGG 3 cut(s) 368, 709, 765
FaeI CATG 1 cut(s) 52
FaiI YATR 6 cut(s) 9, 50, 287, 310, 407, 434
FaqI GGGAC 1 cut(s) 541
FatI CATG 1 cut(s) 48
FauI CCCGC 2 cut(s) 95, 699
FblI GTMKAC 1 cut(s) 360
FokI GGATG 5 cut(s) 70, 154, 470, 626, 743
FspBI CTAG 5 cut(s) 125, 351, 527, 546, 743
GsaI CCCAGC 3 cut(s) 59, 342, 562
GsuI CTGGAG 1 cut(s) 352
HaeIII GGCC 3 cut(s) 321, 569, 770
HapII CCGG 3 cut(s) 304, 464, 601
HgaI GACGC 1 cut(s) 593
Hin1I GRCGYC 1 cut(s) 585
Hin1II CATG 1 cut(s) 52
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 454
HinfI GANTC 6 cut(s) 93, 103, 247, 267, 362, 750
HpaII CCGG 3 cut(s) 304, 464, 601
HphI GGTGA 4 cut(s) 19, 583, 590, 745
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 2 cut(s) 76, 283
Hpy188III TCNNGA 4 cut(s) 22, 205, 226, 665
Hpy8I GTNNAC 1 cut(s) 361
Hpy99I CGWCG 1 cut(s) 176
HpyAV CCTTC 2 cut(s) 160, 175
HpyCH4III ACNGT 2 cut(s) 262, 722
HpyCH4IV ACGT 1 cut(s) 541
HpyF10VI GCNNNNNNNGC 5 cut(s) 327, 330, 339, 453, 469
HpySE526I ACGT 1 cut(s) 541
Hsp92I GRCGYC 1 cut(s) 585
Hsp92II CATG 1 cut(s) 52
KroI GCCGGC 1 cut(s) 463
KroNI GCCGGC 1 cut(s) 465
Kzo9I GATC 7 cut(s) 24, 378, 646, 661, 667, 671, 687
LmnI GCTCC 3 cut(s) 436, 512, 629
Lsp1109I GCAGC 6 cut(s) 158, 342, 447, 484, 608, 726
LweI GCATC 1 cut(s) 563
MaeI CTAG 5 cut(s) 125, 351, 527, 546, 743
MaeII ACGT 1 cut(s) 541
MaeIII GTNAC 2 cut(s) 596, 722
MalI GATC 7 cut(s) 26, 380, 648, 663, 669, 673, 689
MbiI CCGCTC 1 cut(s) 507
MboI GATC 7 cut(s) 24, 378, 646, 661, 667, 671, 687
MboII GAAGA 3 cut(s) 188, 193, 666
MflI RGATCY 1 cut(s) 687
MlsI TGGCCA 1 cut(s) 569
MluCI AATT 4 cut(s) 109, 128, 275, 299
MluNI TGGCCA 1 cut(s) 569
MlyI GAGTC 1 cut(s) 356
MmeI TCCRAC 2 cut(s) 195, 729
MnlI CCTC 8 cut(s) 29, 40, 180, 376, 502, 647, 689, 770
Mox20I TGGCCA 1 cut(s) 569
MroNI GCCGGC 1 cut(s) 463
MscI TGGCCA 1 cut(s) 569
MseI TTAA 1 cut(s) 344
Msp20I TGGCCA 1 cut(s) 569
MspA1I CMGCKG 1 cut(s) 565
MspCI CTTAAG 1 cut(s) 343
MspI CCGG 3 cut(s) 304, 464, 601
MspR9I CCNGG 3 cut(s) 370, 711, 767
MvaI CCWGG 3 cut(s) 370, 711, 767
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 5 cut(s) 327, 330, 339, 453, 469
NaeI GCCGGC 1 cut(s) 465
NdeII GATC 7 cut(s) 24, 378, 646, 661, 667, 671, 687
NgoMIV GCCGGC 1 cut(s) 463
NheI GCTAGC 1 cut(s) 742
NlaIII CATG 1 cut(s) 52
NmuCI GTSAC 1 cut(s) 596
NruI TCGCGA 1 cut(s) 23
PaqCI CACCTGC 1 cut(s) 783
PdiI GCCGGC 1 cut(s) 465
PfeI GAWTC 5 cut(s) 93, 103, 247, 267, 750
PflMI CCANNNNNTGG 1 cut(s) 565
PleI GAGTC 1 cut(s) 356
PpsI GAGTC 1 cut(s) 356
PshAI GACNNNNGTC 1 cut(s) 553
Psp6I CCWGG 3 cut(s) 368, 709, 765
PspFI CCCAGC 3 cut(s) 55, 338, 558
PspGI CCWGG 3 cut(s) 368, 709, 765
PspPI GGNCC 1 cut(s) 713
PstNI CAGNNNCTG 1 cut(s) 565
PsuI RGATCY 1 cut(s) 687
RruI TCGCGA 1 cut(s) 23
RsaI GTAC 2 cut(s) 427, 544
RsaNI GTAC 2 cut(s) 426, 543
SalI GTCGAC 1 cut(s) 359
SaqAI TTAA 1 cut(s) 344
Sau3AI GATC 7 cut(s) 24, 378, 646, 661, 667, 671, 687
Sau96I GGNCC 1 cut(s) 713
SchI GAGTC 1 cut(s) 356
ScrFI CCNGG 3 cut(s) 370, 711, 767
SetI ASST 9 cut(s) 32, 344, 350, 374, 458, 494, 544, 623, 797
SfaNI GCATC 1 cut(s) 563
SinI GGWCC 1 cut(s) 713
SmlI CTYRAG 1 cut(s) 343
SmoI CTYRAG 1 cut(s) 343
Sse9I AATT 4 cut(s) 109, 128, 275, 299
SspMI CTAG 5 cut(s) 125, 351, 527, 546, 743
StyD4I CCNGG 3 cut(s) 368, 709, 765
TaaI ACNGT 2 cut(s) 262, 722
TaiI ACGT 1 cut(s) 544
TaqI TCGA 4 cut(s) 153, 360, 664, 670
TasI AATT 4 cut(s) 109, 128, 275, 299
TauI GCSGC 9 cut(s) 324, 327, 330, 478, 499, 507, 565, 579, 784
TfiI GAWTC 5 cut(s) 93, 103, 247, 267, 750
Tru1I TTAA 1 cut(s) 344
Tru9I TTAA 1 cut(s) 344
TscAI CASTG 1 cut(s) 506
TseFI GTSAC 1 cut(s) 596
TseI GCWGC 7 cut(s) 146, 330, 460, 472, 621, 739, 784
Tsp45I GTSAC 1 cut(s) 596
TspDTI ATGAA 2 cut(s) 34, 312
TspRI CASTG 1 cut(s) 506
Van91I CCANNNNNTGG 1 cut(s) 565
Vha464I CTTAAG 1 cut(s) 343
VpaK11BI GGWCC 1 cut(s) 713
XagI CCTNNNNNAGG 1 cut(s) 764
XapI RAATTY 3 cut(s) 109, 128, 299
XmiI GTMKAC 1 cut(s) 360
XspI CTAG 5 cut(s) 125, 351, 527, 546, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.