Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003280

Physical Location & Seq

Genomic Coordinates Forward (+)

736867 .. 738468

Gene Structure 1602 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1006100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1017 bp

ATGAGTTTTAATAATTGGAGAGAAACCTTTTTTGCTACACTACTGTTTGTAGCAATACTGATGTCTGATTCAAGTGCTTTTCAAGCATTTGAAGAGTGGACTGCGGTGCACGGCCGCATCTACTCCAGCACTCAAGAACGAGAGCTGCGACTCACAATATTCAAAGCCAACTTTGAATTTGTGGAGAAATTTAACAAGAATGGGAAAGGTTTTACCGTCGGCCTCAATCATTTTGCCGACTTGACTACTGAAGAGATCATGAACGGGTACTGCTGTAGACTATTGCAGTCTGATTCGGCCACTAGCATGACAATCTCAGACAATGCTGTTGCTGTCGAAAAAATCGACAACAGTACATGCAGCAGCGTGGATTGGGTCGAGTACGGAGTAGTAACTCCAGTAAGGAATCAGAAAAAATGTGGAAGTTGTTGGGCGTTTGCCGCAGTAACCGGAATAGAAGGAATTTTCGGAATAAAAAGGGGGAAGGACAAGGTGGTGCCGCTGTCTCCTCAGCAGCTTGTGGACTGTGACGAGGTTAGTTATGGCTGCAAGGATGGCCTCGAGGTTCACGCTTATGACTATGTCTCCAAGAGAGGGATCGTAAGTGAGGCCAGTTATCCCTACAAGGCAAAGACCGGCAAATGCAACCCAGAGATATTTGAAAGTCTACCCATTGTCACCTGGATTAATCGCAGTCACAGTGTGTTGCCGAAGGACGAGCAGGCCCTGACAAGGGCTGTGTGTCAACAGCCAGTGACTGTCGGAATTGATGCCGGAAGCGCAGCTTTTGCATATTACAAAGGCGGGATTTTTAGAGATGAATGTGGTACTAATTTGACACACAGTGTCACCATAGTTGGTATCGGGAGGGACGAGTCTGGCACCGAATACTGGAAAATCAAGAATTCCTGGGGCAAGGAATGGGGAGAGGGAGGCTACATGAAACTTCTTAAAGATGGTAGCCCGAATGGACACTGTGGCATTACCATGGATGCCACCTATCCAGTGATTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418

Pfam Domains

Protein Families

Inhibitor_I29 Peptidase_C1

Protein Analysis

339

Amino Acids

37.45

Weight (kDa)

5.51

Isoelectric Point (pI)

28.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	30 - 86	1.5e-17	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	121 - 336	1.5e-64	Papain family cysteine protease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003280)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g32080
malus_domestica	MD12G1005400.v1.1 MD12G1005800.v1.1 MD12G1005900.v1.1 MD12G1006100.v1.1
rosa_chinensis	RchiOBHm_Chr1g0315921 RchiOBHm_Chr7g0214921
rosa_laevigata	RLG00000002708 RLG00000029343 RLG00000030723
rosa_multiflora	Rmu_co8234753.1_g000001 Rmu_sc0002308.1_g000003 Rmu_sc0002886.1_g000001 Rmu_sc0004614.1_g000001 Rmu_sc0006316.1_g000002 Rmu_sc0007921.1_g000002 Rmu_sc0012813.1_g000001 Rmu_ssc0000134.1_g000025 Rmu_ssc0000154.1_g000024
rosa_roxburghii	Rroxscaffold_3G00244880 Rroxscaffold_4G00315430 Rroxscaffold_4G00331310
rosa_rugosa	Rorug01G0007000 Rorug04G0097100 Rorug07G0148700
rosa_samantha	Rh1AG017800 Rh1AG148200 Rh1DG010800 Rh1DG154900 Rh7AG285500 Rh7AG294300 Rh7BG285900 Rh7CG314300
rosa_wichuraiana	Rw1G001080 Rw1G011970 Rw7G024360 Rw7G025400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	560
AccB1I	GGYRCC	2 cut(s)	496, 881
AccI	GTMKAC	2 cut(s)	277, 667
AciI	CCGC	5 cut(s)	104, 115, 441, 500, 804
AclWI	GGATC	1 cut(s)	605
AcoI	YGGCCR	2 cut(s)	112, 297
AcsI	RAATTY	4 cut(s)	176, 188, 462, 904
AcuI	CTGAAG	1 cut(s)	270
AdeI	CACNNNGTG	2 cut(s)	703, 845
AfaI	GTAC	4 cut(s)	269, 355, 383, 829
AfiI	CCNNNNNNNGG	4 cut(s)	594, 732, 733, 891
AgsI	TTSAA	6 cut(s)	72, 83, 92, 163, 176, 662
AjnI	CCWGG	2 cut(s)	680, 908
AluBI	AGCT	3 cut(s)	145, 517, 785
AluI	AGCT	3 cut(s)	145, 517, 785
Alw21I	GWGCWC	1 cut(s)	111
Alw26I	GTCTC	2 cut(s)	510, 589
Alw44I	GTGCAC	1 cut(s)	107
AlwI	GGATC	1 cut(s)	605
AlwNI	CAGNNNCTG	2 cut(s)	727, 758
Ama87I	CYCGRG	1 cut(s)	560
AoxI	GGCC	6 cut(s)	112, 220, 297, 556, 609, 723
ApaLI	GTGCAC	1 cut(s)	107
ApeKI	GCWGC	6 cut(s)	145, 360, 363, 514, 546, 782
ApoI	RAATTY	4 cut(s)	176, 188, 462, 904
AseI	ATTAAT	1 cut(s)	687
AspLEI	GCGC	1 cut(s)	782
AspS9I	GGNCC	1 cut(s)	724
AsuHPI	GGTGA	2 cut(s)	670, 841
AvaI	CYCGRG	1 cut(s)	560
BaeGI	GKGCMC	1 cut(s)	111
BaeI	ACNNNNGTAYC	2 cut(s)	259, 292
BanI	GGYRCC	2 cut(s)	496, 881
Bbv12I	GWGCWC	1 cut(s)	111
BbvCI	CCTCAGC	1 cut(s)	510
BbvI	GCAGC	6 cut(s)	132, 372, 375, 526, 533, 794
BccI	CCATC	2 cut(s)	548, 950
BceAI	ACGGC	1 cut(s)	127
BciT130I	CCWGG	2 cut(s)	682, 910
BcoDI	GTCTC	2 cut(s)	510, 589
BfaI	CTAG	1 cut(s)	303
BfmI	CTRYAG	1 cut(s)	274
BisI	GCNGC	9 cut(s)	115, 146, 361, 364, 441, 500, 515, 547, 783
BlsI	GCNGC	9 cut(s)	116, 147, 362, 365, 442, 501, 516, 548, 784
Bme1390I	CCNGG	2 cut(s)	682, 910
BmeT110I	CYCGRG	1 cut(s)	560
BmgT120I	GGNCC	1 cut(s)	724
BmiI	GGNNCC	2 cut(s)	498, 883
BmrFI	CCNGG	2 cut(s)	682, 910
BmsI	GCATC	3 cut(s)	126, 760, 982
BpmI	CTGGAG	2 cut(s)	109, 381
Bpu10I	CCTNAGC	1 cut(s)	510
BpuEI	CTTGAG	1 cut(s)	117
BsaJI	CCNNGG	2 cut(s)	909, 987
BsaWI	WCCGGW	1 cut(s)	449
BsaXI	ACNNNNNCTCC	2 cut(s)	569, 599
Bsc4I	CCNNNNNNNGG	4 cut(s)	594, 732, 733, 891
Bse118I	RCCGGY	1 cut(s)	635
Bse1I	ACTGG	5 cut(s)	398, 612, 752, 896, 1004
BseBI	CCWGG	2 cut(s)	682, 910
BseDI	CCNNGG	2 cut(s)	909, 987
BseGI	GGATG	2 cut(s)	559, 997
BseLI	CCNNNNNNNGG	4 cut(s)	594, 732, 733, 891
BseMII	CTCAG	2 cut(s)	330, 524
BseNI	ACTGG	5 cut(s)	398, 612, 752, 896, 1004
BseRI	GAGGAG	1 cut(s)	498
BseSI	GKGCMC	1 cut(s)	111
BseX3I	CGGCCG	1 cut(s)	112
BseXI	GCAGC	6 cut(s)	132, 372, 375, 526, 533, 794
Bsh1285I	CGRYCG	1 cut(s)	115
BshFI	GGCC	6 cut(s)	114, 222, 299, 558, 611, 725
BshNI	GGYRCC	2 cut(s)	496, 881
BsiEI	CGRYCG	1 cut(s)	115
BsiHKAI	GWGCWC	1 cut(s)	111
BsiHKCI	CYCGRG	1 cut(s)	560
BsiSI	CCGG	3 cut(s)	450, 636, 774
BslFI	GGGAC	1 cut(s)	884
BslI	CCNNNNNNNGG	4 cut(s)	594, 732, 733, 891
BsmAI	GTCTC	2 cut(s)	510, 589
BsmFI	GGGAC	1 cut(s)	884
BsnI	GGCC	6 cut(s)	114, 222, 299, 558, 611, 725
BsoBI	CYCGRG	1 cut(s)	560
Bsp1286I	GDGCHC	1 cut(s)	111
Bsp143I	GATC	2 cut(s)	255, 597
Bsp19I	CCATGG	1 cut(s)	987
BspACI	CCGC	5 cut(s)	104, 115, 441, 500, 804
BspANI	GGCC	6 cut(s)	114, 222, 299, 558, 611, 725
BspCNI	CTCAG	2 cut(s)	329, 523
BspHI	TCATGA	1 cut(s)	258
BspLI	GGNNCC	2 cut(s)	498, 883
BspPI	GGATC	1 cut(s)	605
BspT107I	GGYRCC	2 cut(s)	496, 881
BsrFI	RCCGGY	1 cut(s)	635
BsrI	ACTGG	5 cut(s)	398, 612, 752, 896, 1004
BssAI	RCCGGY	1 cut(s)	635
BssECI	CCNNGG	2 cut(s)	909, 987
BssMI	GATC	2 cut(s)	255, 597
BssT1I	CCWWGG	1 cut(s)	987
Bst2UI	CCWGG	2 cut(s)	682, 910
Bst4CI	ACNGT	8 cut(s)	45, 217, 353, 527, 701, 760, 845, 977
Bst6I	CTCTTC	2 cut(s)	87, 246
BstAPI	GCANNNNNTGC	1 cut(s)	788
BstC8I	GCNNGC	1 cut(s)	723
BstDEI	CTNAG	2 cut(s)	316, 510
BstDSI	CCRYGG	1 cut(s)	987
BstF5I	GGATG	2 cut(s)	559, 997
BstHHI	GCGC	1 cut(s)	782
BstKTI	GATC	2 cut(s)	258, 600
BstMAI	GTCTC	2 cut(s)	510, 589
BstMBI	GATC	2 cut(s)	255, 597
BstMCI	CGRYCG	1 cut(s)	115
BstMWI	GCNNNNNNNGC	5 cut(s)	83, 440, 555, 779, 788
BstNI	CCWGG	2 cut(s)	682, 910
BstNSI	RCATGY	1 cut(s)	360
BstSCI	CCNGG	2 cut(s)	680, 908
BstSFI	CTRYAG	1 cut(s)	274
BstSLI	GKGCMC	1 cut(s)	111
BstV1I	GCAGC	6 cut(s)	132, 372, 375, 526, 533, 794
BstZI	CGGCCG	1 cut(s)	112
BsuRI	GGCC	6 cut(s)	114, 222, 299, 558, 611, 725
BtgI	CCRYGG	1 cut(s)	987
BtsCI	GGATG	2 cut(s)	559, 997
BtsIMutI	CAGTG	5 cut(s)	706, 759, 850, 973, 1011
Cac8I	GCNNGC	1 cut(s)	723
CaiI	CAGNNNCTG	2 cut(s)	727, 758
CciI	TCATGA	1 cut(s)	258
CfoI	GCGC	1 cut(s)	782
Cfr10I	RCCGGY	1 cut(s)	635
Cfr13I	GGNCC	1 cut(s)	724
Csp6I	GTAC	4 cut(s)	268, 354, 382, 828
CviAII	CATG	5 cut(s)	259, 307, 357, 940, 988
CviQI	GTAC	4 cut(s)	268, 354, 382, 828
DdeI	CTNAG	2 cut(s)	316, 510
DpnI	GATC	2 cut(s)	257, 599
DpnII	GATC	2 cut(s)	255, 597
DraIII	CACNNNGTG	2 cut(s)	703, 845
EaeI	YGGCCR	2 cut(s)	112, 297
EagI	CGGCCG	1 cut(s)	112
Eam1104I	CTCTTC	2 cut(s)	87, 246
EarI	CTCTTC	2 cut(s)	87, 246
EclXI	CGGCCG	1 cut(s)	112
Eco130I	CCWWGG	1 cut(s)	987
Eco52I	CGGCCG	1 cut(s)	112
Eco57I	CTGAAG	1 cut(s)	270
Eco88I	CYCGRG	1 cut(s)	560
EcoO109I	RGGNCCY	1 cut(s)	724
EcoRI	GAATTC	1 cut(s)	904
EcoRII	CCWGG	2 cut(s)	680, 908
EcoT14I	CCWWGG	1 cut(s)	987
ErhI	CCWWGG	1 cut(s)	987
FaeI	CATG	5 cut(s)	262, 310, 360, 943, 991
FalI	AAGNNNNNCTT	2 cut(s)	769, 801
FaqI	GGGAC	1 cut(s)	884
FatI	CATG	5 cut(s)	258, 306, 356, 939, 987
FauI	CCCGC	1 cut(s)	797
FblI	GTMKAC	2 cut(s)	277, 667
Fnu4HI	GCNGC	9 cut(s)	115, 146, 361, 364, 441, 500, 515, 547, 783
FokI	GGATG	2 cut(s)	566, 1004
Fsp4HI	GCNGC	9 cut(s)	115, 146, 361, 364, 441, 500, 515, 547, 783
FspBI	CTAG	1 cut(s)	303
GlaI	GCGC	1 cut(s)	781
GluI	GCNGC	9 cut(s)	115, 146, 361, 364, 441, 500, 515, 547, 783
GsuI	CTGGAG	2 cut(s)	109, 381
HaeIII	GGCC	6 cut(s)	114, 222, 299, 558, 611, 725
HapII	CCGG	3 cut(s)	450, 636, 774
HhaI	GCGC	1 cut(s)	782
Hin1II	CATG	5 cut(s)	262, 310, 360, 943, 991
Hin6I	GCGC	1 cut(s)	780
HinP1I	GCGC	1 cut(s)	780
HincII	GTYRAC	1 cut(s)	746
HindII	GTYRAC	1 cut(s)	746
HinfI	GANTC	5 cut(s)	68, 150, 293, 406, 875
HpaII	CCGG	3 cut(s)	450, 636, 774
HphI	GGTGA	2 cut(s)	670, 841
Hpy166II	GTNNAC	7 cut(s)	99, 109, 278, 523, 568, 668, 746
Hpy188I	TCNGA	6 cut(s)	67, 292, 319, 411, 470, 764
Hpy188III	TCNNGA	4 cut(s)	134, 259, 865, 901
Hpy8I	GTNNAC	7 cut(s)	99, 109, 278, 523, 568, 668, 746
Hpy99I	CGWCG	1 cut(s)	221
HpyAV	CCTTC	3 cut(s)	452, 478, 706
HpyCH4III	ACNGT	8 cut(s)	45, 217, 353, 527, 701, 760, 845, 977
HpyCH4V	TGCA	6 cut(s)	109, 286, 360, 549, 645, 791
HpyF10VI	GCNNNNNNNGC	5 cut(s)	83, 440, 555, 779, 788
HpyF3I	CTNAG	2 cut(s)	316, 510
Hsp92II	CATG	5 cut(s)	262, 310, 360, 943, 991
HspAI	GCGC	1 cut(s)	780
Kzo9I	GATC	2 cut(s)	255, 597
Lsp1109I	GCAGC	6 cut(s)	132, 372, 375, 526, 533, 794
LweI	GCATC	3 cut(s)	126, 760, 982
MaeI	CTAG	1 cut(s)	303
MaeIII	GTNAC	7 cut(s)	391, 445, 527, 676, 695, 754, 847
MalI	GATC	2 cut(s)	257, 599
MboI	GATC	2 cut(s)	255, 597
MboII	GAAGA	2 cut(s)	104, 263
MhlI	GDGCHC	1 cut(s)	111
MluCI	AATT	7 cut(s)	13, 176, 188, 462, 765, 832, 904
MlyI	GAGTC	2 cut(s)	144, 884
MmeI	TCCRAC	1 cut(s)	742
MseI	TTAA	4 cut(s)	9, 192, 687, 951
MslI	CAYNNNNRTG	3 cut(s)	305, 573, 986
MspA1I	CMGCKG	1 cut(s)	502
MspI	CCGG	3 cut(s)	450, 636, 774
MspR9I	CCNGG	2 cut(s)	682, 910
MvaI	CCWGG	2 cut(s)	682, 910
MwoI	GCNNNNNNNGC	5 cut(s)	83, 440, 555, 779, 788
NcoI	CCATGG	1 cut(s)	987
NdeII	GATC	2 cut(s)	255, 597
NlaIII	CATG	5 cut(s)	262, 310, 360, 943, 991
NlaIV	GGNNCC	2 cut(s)	498, 883
NmuCI	GTSAC	5 cut(s)	527, 676, 695, 754, 847
NspI	RCATGY	1 cut(s)	360
PaeR7I	CTCGAG	1 cut(s)	560
PagI	TCATGA	1 cut(s)	258
PcsI	WCGNNNNNNNCGW	3 cut(s)	145, 342, 870
PfeI	GAWTC	3 cut(s)	68, 293, 406
PflFI	GACNNNGTC	1 cut(s)	581
PkrI	GCNGC	9 cut(s)	116, 147, 362, 365, 442, 501, 516, 548, 784
PleI	GAGTC	2 cut(s)	144, 883
PpsI	GAGTC	2 cut(s)	144, 883
PshBI	ATTAAT	1 cut(s)	687
Psp6I	CCWGG	2 cut(s)	680, 908
PspGI	CCWGG	2 cut(s)	680, 908
PspN4I	GGNNCC	2 cut(s)	498, 883
PspPI	GGNCC	1 cut(s)	724
PspXI	VCTCGAGB	1 cut(s)	560
PstNI	CAGNNNCTG	2 cut(s)	727, 758
PsyI	GACNNNGTC	1 cut(s)	581
RsaI	GTAC	4 cut(s)	269, 355, 383, 829
RsaNI	GTAC	4 cut(s)	268, 354, 382, 828
RseI	CAYNNNNRTG	3 cut(s)	305, 573, 986
SaqAI	TTAA	4 cut(s)	9, 192, 687, 951
SatI	GCNGC	9 cut(s)	115, 146, 361, 364, 441, 500, 515, 547, 783
Sau3AI	GATC	2 cut(s)	255, 597
Sau96I	GGNCC	1 cut(s)	724
SchI	GAGTC	2 cut(s)	144, 884
ScrFI	CCNGG	2 cut(s)	682, 910
SduI	GDGCHC	1 cut(s)	111
SfaNI	GCATC	3 cut(s)	126, 760, 982
SfcI	CTRYAG	1 cut(s)	274
Sfr274I	CTCGAG	1 cut(s)	560
SlaI	CTCGAG	1 cut(s)	560
SmiMI	CAYNNNNRTG	3 cut(s)	305, 573, 986
SmlI	CTYRAG	2 cut(s)	132, 560
SmoI	CTYRAG	2 cut(s)	132, 560
Sse9I	AATT	7 cut(s)	13, 176, 188, 462, 765, 832, 904
SsiI	CCGC	5 cut(s)	104, 115, 441, 500, 804
SspI	AATATT	1 cut(s)	159
SspMI	CTAG	1 cut(s)	303
StyD4I	CCNGG	2 cut(s)	680, 908
StyI	CCWWGG	1 cut(s)	987
TaaI	ACNGT	8 cut(s)	45, 217, 353, 527, 701, 760, 845, 977
TaqI	TCGA	4 cut(s)	336, 345, 378, 561
TasI	AATT	7 cut(s)	13, 176, 188, 462, 765, 832, 904
TatI	WGTACW	1 cut(s)	353
TauI	GCSGC	3 cut(s)	117, 443, 502
TfiI	GAWTC	3 cut(s)	68, 293, 406
Tru1I	TTAA	4 cut(s)	9, 192, 687, 951
Tru9I	TTAA	4 cut(s)	9, 192, 687, 951
TscAI	CASTG	5 cut(s)	706, 759, 850, 980, 1011
TseFI	GTSAC	5 cut(s)	527, 676, 695, 754, 847
TseI	GCWGC	6 cut(s)	145, 360, 363, 514, 546, 782
Tsp45I	GTSAC	5 cut(s)	527, 676, 695, 754, 847
TspDTI	ATGAA	3 cut(s)	275, 834, 956
TspGWI	ACGGA	1 cut(s)	399
TspRI	CASTG	5 cut(s)	706, 759, 850, 980, 1011
Tth111I	GACNNNGTC	1 cut(s)	581
VneI	GTGCAC	1 cut(s)	107
VspI	ATTAAT	1 cut(s)	687
XapI	RAATTY	4 cut(s)	176, 188, 462, 904
XceI	RCATGY	1 cut(s)	360
XhoI	CTCGAG	1 cut(s)	560
XmiI	GTMKAC	2 cut(s)	277, 667
XspI	CTAG	1 cut(s)	303

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.